| the role of mite allergen in chronic urticaria. | fifty-two patients with chronic urticaria were tested intradermally with mite allergen (dermatophagoides farinae). none had any past history of any other atopic diseases. thirty of the 52 patients gave positive skin tests to mite allergen. a good correlation between skin sensitivity to mite allergen and in vitro mite antigen-induced histamine release from leucocytes was observed. these facts suggest that a mite may be an important role in chronic urticaria. | 1979 | 92903 |
| clinical correlations between long-term (ige) and short-term (igg s-ts) anaphylactic antibodies in atopic and 'non-atopic' subjects with respiratory allergic disease. | the sera of atopic and non-atopic persons with allergic pulmonary disorders were examined for long-term sensitizing, ige and short-term sensitizing heat-stable (s-ts) antibodies which were present separately or together in the sera of some patients sensitive to antigens such as budgerigar serum proteins and aspergillus funigatus. in fourteen atopic patients with extrinsic asthma, six had both types of antibody to common allergens, and of nine non-atopic patients with crytogenic (intrinsic) asthm ... | 1979 | 117951 |
| characterization of the allergenic components of the house dust mite, dermatophagoides farinae. | characterization and purification of the allergenic components of the house dust mite, dermatophagoides farinae, were investigated. it was found that the mite antigen was heat-stable, pronase-sensitive, and periodate-resistant, which suggested that the antigenic determinants of the mite antigen resided mainly in the protein component. purification of the mite antigen was tried using sephadex g-150, deae cellulose, and isoelectrofocusing columns. the most potent allergenic fraction was around 20, ... | 1977 | 201571 |
| specific ige estimations by rast in japanese asthmatics compared with skin, passive transfer and bronchial provocation tests. | 198 asthmatics and twenty healthy persons were studied by rast and in vivo tests with four common inhalant allergens. (a) higher rast classes were elicited with mite (dermatophagoides farinae) extract and lower classes with other allergens. the agreement between positive rast and skin or p-k tests was highest with the mite extract. rast sensitivity was dependent on the kinds of allergens and was most sensitive to mite extract. positive rast was most closely related to the prick test reactions. ( ... | 1978 | 348345 |
| mites in house dust in the stockholm area. | today there is no doubt that mites (and especially species within the pyroglyphidae family: dermatophagoides pteronyssinus and dermatophagoides farinae) are an important agent in dust allergies. the author reports on a study of the occurrence of mites in house dust in the environment of allergic persons in the stockholm area. 201 private homes, 13 farmers' houses and two hospitals have been studied. in 12% of the homes several different types of mite were found in dust in relatively small number ... | 1979 | 378014 |
| house dust, mite (d. farinae) and cockroach allergy in a midwestern population. | by using three different skin testing techniques and rast, this study explores the relationship among house dust, mite (dermatophagoides farinae) and cockroach allergy in atopic patients in the kansas city area. results suggest that the cockroach antigen may be as important as the mite in the etiology of house dust allergy in this population. | 1979 | 426325 |
| [treatment with extract of dermatophagoides farinae in asthmatics: a four-year observation (author's transl)]. | | 1979 | 551901 |
| selective isolation of allergens. i. reaction of house dust mite extracts with tridacnin and concanavalin a and examination of the allergenicity of the isolated components. | tridacnin, a lectin from the clam tridacna maxima was found to precipitate with crude extracts from the house dust mites dermatophagoides farinae and dermatophagoides pteronyssinus. using tridacnin in direct precipitation and affinity chromatography studies, a carbohydrate-rich preparation of high molecular weight was isolated from d. farinae extracts. the isolated preparation gave one precipitin line when used with tridacnin in gel diffusion and immunoelectrophoresis experiments but two bands w ... | 1977 | 589778 |
| [dermatophagoides pteronyssinus and dermatophagoides farinae from house dust in columbia]. | | 1977 | 605992 |
| humidity as a factor regulating feeding and water balance of the house dust mites dermatophagoides farinae and d. pteronyssinus (acari: pyroglyphidae). | | 1977 | 609080 |
| dermatophagoides farinae antigen skin test sensitivity in wisconsin. | fifty consecutive patients with allergic asthma and/or rhinitis in madison. wisconsin, were tested intradermally with a house dust extract (1:100) and with a dermatophagoides farinae antigen (1:2000). the close relationship between the two skin test results (mite and house dust) suggests that both contain the same cross-reacting antigens and that mites make a major contribution to the antigens in house dust in wisconsin. | 1978 | 717849 |
| [ecological study of pyroglyphides mites in house dust in the grenoble area. a qualitative approach and incidence of various parameters: seasons, altitude, temperature and relative dampness in their multiplication]. | this study concerns the analysis of 70 samples of dust mites taken periodically from 13 sample areas or mattresses at different altitudes. it shows the presence of pyroglyphid mites in all the mattresses studied. four species were found: dermatophagoides pteronyssinus, dermatophagoides farinae, euroglyphus maynei and dermatophagoides evansi. from a quantitative point of view, the statistical analysis of the results obtained allows the following conclusions to be drawn: -- all the pyroglyphids (a ... | 1978 | 754622 |
| [hypersensitivity to dermatophagoides farinae compared with an extract of housedust by the prick-test technic in asthmatic and non-asthmatic children in santiago and osorno]. | | 1978 | 757009 |
| mortality of house dust mites, dermatophagoides farinae and d. pteronyssinus, exposed to dehydrating conditions or selected pesticides. | | 1976 | 796455 |
| antigenic and allergenic constituents of dermatophagoides pteronyssinus extract. | the antigenic analysis of dermatophagoides pteronyssinus extract was performed by using antiserum obtained in rabbits by immunization with mite extract in freund's adjuvant. immunodiffusion and immunoelectrophoretic analysis revealed the presence of many constituents, most of which were common to human dander, house dust and dermatophagoides farinae extracts. by fractionation of the mite extract on sephadex g-75 and deae-sephadex it was shown that the highest allergenic activity was related to a ... | 1975 | 807491 |
| precipitin to mite detected in asthmatic patients. | mite antigen from dermatophagoides farinae was labelled with 125i, and used for detection of precipitin to mite antigen in sera from asthmatic patients allergic to mite and house dust. by double gel diffusion using this labelled antigen, it was found that 16 out of 37 sera formed precipitin bands which were detected by radioautography. only one serum formed a precipitin band when non-labelled mite antigen was used in double gel diffusion. the main immunogenic component in mite extract migrated ... | 1975 | 812327 |
| skin tests in nigerian asthmatics from the equatorial forest zone in benin, nigeria. | forty-two patients with bronchial asthma from the benin area and 32 control subjects were subjected to skin prick tests for groups of allergens. there was a significant excess of reactions of 5 mm or over to dermatophagoides farinae amongst the asthmatics. this was the most commonly detected allergen. there was an overall excess in asthmatics of positive reactions of over 5 mm in the other groups although there was no significant excess in any single group. there was no significant difference in ... | 1977 | 871031 |
| a case of postimaginal molt in the american house dust mite dermatophagoides farinae hughes 1961 (acari: pyroglyphidae). | | 1976 | 952188 |
| permeability to water in different adaptive phases of the same instar in the american house-dust mite. | | 1976 | 952189 |
| tyrophagus putrescentiae (acarina: tyroglyphidae) in cultures of dermatophagoides farinae (acarina: pyroglyphidae) controlled by low humidity. | | 1976 | 978699 |
| effects on the jh-analogues altosid and altozar on the north american house-dust mite, dermatophagoides farinae hughes (acarina, pyroglyphidae). | | 1976 | 1020636 |
| bronchial asthma in the nigerian savanna region. a clinical and laboratory study of 106 patients with a review of the literature on asthma in the tropics. | one hundred and six asthma patients were studied in zaria in the nigerian savanna region. this group resembled hospital attenders in general in containing a disproportionately large number of immigrants from southern nigeria and students undergoing higher education. childhood asthma was rare. asthma started after the age of 19 years in 69 per cent of patients. twenty-seven per cent gave a history of rhinitis but none had had eczema. twenty-two per cent gave a family history of asthma. cutaneous ... | 1975 | 1101285 |
| an analysis of skin prick test reactions in 656 asthmatic patients. | of 656 asthmatic patients referred specifically for allergy assessments, 544 (84 percent) gave positive immediate skin prick tests to at least one of 22 common allergens used routinely. comparison of these skin test positive patients with the 102 (16 percent) who were skin test negative showed a number of significant differences. the majority of the skin test positive patients (52 percent) were less than 10 years old at the time of onset of the asthma, whereas, of the skin test negative patients ... | 1975 | 1168378 |
| [recent studies on nasal allergy (vii). the changes of the serum ige level and specific ige antibodies in patients with nasal allergy by hyposensitization therapy using mite (dermatophagoides farinae) antigen (author's transl)]. | | 1975 | 1172529 |
| enzymatic hydrolysis of allergens extracted from the house dust mite, dermatophagoides farinae. | the crude extract of the house dust mite, dermatophagoides farinae, was subjected to enzymatic hydrolysis by the use of trypsin and pronase. the hydrolysate was fractionated by gel-filtration and ion-exchange chromatographies. allergenic activities of the fractions were measured by prausnitz-küstner type skin test on guinea pig skin with homocytotropic antibodies. major allergenic component passed through anion and cation dowex resins but its yield was not large. on gel-filtration, the mite dige ... | 1975 | 1185896 |
| population structure and dynamics of the house dust mite dermatophagoides farinae (acarina: pyroglyphidae) in czechoslovakia. | the relationship between the room microclimate and population dynamics of the house dust mite dermatophagoides farinae hughes, 1961 was studied. a distinct increase of the mite population density in mattresses was observed only in the period, when the monthly means of relative air humidity in rooms were higher than 47-50%, denominated by the author as critical air humidity. this is a considerably lower value than that relevant to dermatophagoides pteronyssinus (trouessart, 1897) from the paper b ... | 1975 | 1193484 |
| further studies in allergenic identity between house dust and the house dust mite, dermatophagoides farinae hughes, 1961. | the sera from 99 japanese asthmatic subjects were studied for the allergenic similarity between house dust and the house-dust mite, dermatophagoides farinae hughes, 1961. a close correlation (correlation coefficient 0.92, p less than 0.01) was obtained between house dust and the mite with the radioallergosorbent test (rast). high correlation was not noted between house dust and other allergens such as smoky brown cockroach, periplaneta fuliginosa, aspergillus, book lice, liposcelis bostricophilu ... | 1975 | 1200424 |
| [hyposensitization with extracts of house dust and mites adsorbed on aluminium-hydroxyde (allpyral) in children with bronchial asthma (author's transl)]. | since the year 1971 the authors have treated 39 asthmatic patients with allpyral house dust vaccins containing dermatophagoides farinae extract. 16 of these children previously had similar treatment, but without mite extract, and this was changed because of the poor results. patients allergic only to house dust and mites were selected according to the skin tests. skin reaction to mite extract did not change even in symptomfree children in contrast to the decrease or disappearance of skin sensiti ... | 1976 | 1264078 |
| inhibition of mite protease (df-protease) with protease inhibitors. | a protease from house dust mite(dermatophagoides farinae) having high specificity towards a substrate of blood coagulation factor xiia catalyzes the activation of kallikrein-kinin system in plasma (takahashi et al., 1990). to prevent the formation of kinin by the mite-protease, inhibition of the protease with its inhibitors was tested in vitro and in vivo. its kinetic studies revealed that ki values are 3.9 x 10(-10) m for aprotinin, 3.0 x 10(-9) m for soybean trypsin inhibitor (kunitz) and 2.5 ... | 1992 | 1282814 |
| dust from carpeted and smooth floors. v. cat (fel d i) and mite (der p i and der f i) allergen levels in school dust. demonstration of the basophil histamine release induced by dust from classrooms. | sixty dust samples from schools in norway were analysed for major allergens from cat and mite after sampling with the regularly used vacuum cleaners for 5 days and with a new model vacuum cleaner for 10 days, respectively. the major feline allergen felis domesticus allergen i (fel d i) was detected in all the classrooms, with ranges from 12 to 16,840 ng/m2 floor area. the mean fel d i concentration was about 11 times higher per unit area carpeted floors as compared with smooth floors after the 1 ... | 1992 | 1283110 |
| [elevated production of gm-csf by peripheral blood mononuclear cells stimulated with mite antigens in children with bronchial asthma]. | granulocyte-macrophage colony-stimulating factor (gm-csf) is a cytokine which can stimulate the proliferation and functions of eosinophils and neutrophils. we have studied whether peripheral blood mononuclear cells (pbmc), obtained from children with stable asthma, can produce gm-csf through stimulation with crude dermatophagoides farinae (df) antigen in vitro. the patients had strongly positive rast results for house dust mite df. levels of gm-csf in pbmc culture supernatant were assayed by an ... | 1992 | 1290408 |
| [clinical significance of igg4 antibody determination in children against egg white, milk, soybean and dermatophagoides farinae]. | the measurement of ige and igg4 antibodies against egg white, milk, soybean and dermatophagoides farinae was performed by fast (fluorescence allergosorbent test) using 21 serum samples obtained from non-allergic children and 160 serum samples from atopic children with bronchial asthma and/or atopic dermatitis. their antibody levels were evaluated for any association with disease severity and for clinical significance in establishing diagnosis. it was found that children with bronchial asthma sho ... | 1992 | 1290413 |
| allergic skin reactivity in 232 patients over 50 years. | we examined two hundred and thirty-two out-patients, ranging in age from 50 to 75 yrs. all patients were submitted to skin-tests: prick and, if it was necessary, intradermal reactions and/or rast. allergens employed were gramineae, parietaria, olea, dermatophagoides farinae, dermatophagoides pteronyssinus, house dust, cat and dog skin scales. one hundred and twenty-eight subjects had positive responses to one or more aeroallergens. asthma was the most frequent condition both in skin-positives an ... | 1992 | 1292328 |
| plasma kallikrein amidolytic activity in bronchial asthma. | we have investigated plasma kallikrein amidolytic activity in the following groups of patients: 1. normal control group of blood donors. 2. extrinsic pollen-activated bronchial asthma patients, during periods of symptomatology and at a later time after the spring. 3. subjects with atopic bronchial asthma in acute phase when admitted to our hospital's emergency room and later when clinically recovered. 4. subjects with extrinsic bronchial asthma, sensitive to dermatophagoides pteronyssinus and de ... | 1992 | 1292330 |
| [the effect of a mite allergen on na/h metabolic activity in peritoneal mast cells]. | mite allergen interacting with mast cells treated with sera from bronchial patient sensitized to home dust dermatophagoides farinae causes changes in intracellular ph. regulation of phi peritoneal mast cells is participated by na/h metabolism probably activated by protein kinase c. | 1992 | 1292700 |
| [allergen-stimulated release of prostaglandin e, f2 alpha by alveolar macrophages from patients with allergic asthma]. | bronchoalveolar lavage (bal) was performed in healthy subjects (n = 12) and patients with bronchial asthma (n = 11). between the two groups there was no significant difference in the total number of cells and the percentage of alveolar macrophages (am), lymphocytes (l), neutrophils (n) and eosinophils (e) in bal fluids. when am were cultured in vitro and stimulated with dermatophagoides farinae (dpf) antigen, the amount of pge released by am was significantly increased in the asthmatics. when th ... | 1992 | 1306779 |
| [false positive reaction in measurement of allergen-specific ige--comparison of 3m ige fast-plus test using polystyrene well as adsorbent with phadezym rast]. | irrelevant ige binding to cellulose discs is known to give false positive results in phadezym rast (pharmacia) for the estimation of allergen-specific ige in serum. we investigated fast-plus test (3m diagnostic systems), an enzyme-linked sandwich type fluoro-allergo-sorbent test in which a particular allergen was coated to polystyrene well. phadezym rast and cap rast (pharmacia) using cellulose-derivative discs as adsorbent were used as reference methods. patients' sera which gave negative blank ... | 1992 | 1307629 |
| cloning and expression of cdna coding for the major house dust mite allergen der f ii in escherichia coli. | a cdna library corresponding to mite protein was screened using anti-der f ii, a major allergen from the house dust mite dermatophagoides farinae, antibody. three possible clones were obtained that contained cdna fragments coding for der f ii, and the nucleotide sequences of the fragments were determined. there were minor differences observed affecting the deduced amino acid sequence among the three cdna fragments. the amino acid sequence of the purified native der f ii protein could be analyzed ... | 1991 | 1368682 |
| identification of allergenic epitopes on der f i, a major allergen of dermatophagoides farinae, using monoclonal antibodies. | the antigenic and allergenic structure of der f i, a major allergen of the house dust mite dermatophagoides farinae (df) was investigated by means of a panel of 11 selected monoclonal antibodies (mab) obtained from balb/c mice immunized with purified der f i. the species specificity of these mab, tested with der f i and der p i--the homologous allergen from dermatophagoides pteronyssinus--was generally restricted to der f i since 10 out of 11 mab reacted only with this allergen. epitope specific ... | 1992 | 1371821 |
| monitoring of mite dermatophagoides farinae allergen-specific igg and igg subclass distribution in patients on immunotherapy. | house dust mite d. farinae and der f ii-specific igg and igg subclass responses were evaluated in 32 adults with perennial rhinitis undergoing immunotherapy for 1 year by means of igg-rast and elisa. the elisa method, which is based on subclass-specific monoclonal antibodies, could detect 0.5-1.5 ng/ml of specific antibodies. d. farinae and der f ii-specific igg4 antibodies increased continuously as immunotherapy proceeded, while total igg and igg1 antibodies reached a plateau value 6 months aft ... | 1992 | 1378700 |
| [inhibitory activity of mite igg4, antibody on antigen-induced histamine release from human peripheral blood leukocytes]. | several reports have yielded conflicting results on the role of igg4 antibody on the surfaces of target cells in immediate type allergy. this study was performed to elucidate whether igg4 antibody inhibits ige-mediated histamine release from target cells after antigenic stimulation, and whether it has reaginic activity. serum was obtained from patients with nasal allergy receiving specific immunotherapy for housedust and mites. ige and igg4 were enriched affinity chromatographically using monocl ... | 1992 | 1380985 |
| the allergen specific b-cell response during immunotherapy. | the paper examines the allergen specific b-cell response in peripheral blood from patients undergoing immunotherapy with house dust mite extract. the 12 patients were part of a double blind placebo controlled study, and they were treated with either dermatophagoides pteronyssinus (n = 4), dermatophagoides farinae extract (n = 3) (alutard sq, alk, denmark) or placebo (n = 5). blood was taken every fortnight on day seven after hyposensitization and tested for igm, igg, iga and ige antibody secreti ... | 1992 | 1393764 |
| [sensitization to house dust mites: importance and possibilities of allergen elimination]. | house-dust mite allergy (dermatophagoides pteronissinus and dermatophagoides farinae) is an important causative factor for allergic asthma or rhinitis in children. there is a clear relationship between the degree of allergen exposure and the subsequent development of asthma or the risk of sensitisation. it is therefore useful to know how intensive a patient is exposed to house-dust allergen. this can be managed either by counting all mites in the dust, by measuring of the content of der p i and ... | 1992 | 1434335 |
| is a trypsin-like protease of mites a der f iii allergen? | a trypsin-like protease was purified from mite (dermatophagoides farinae) fecal extract. in sds-page, the mite trypsin-like protease showed a single band at 34 kd. the purified trypsin-like protease possessed potent allergenic activity. both the twenty n-terminal amino acid sequence and the amino acid composition of the purified protease were very similar to those of der f iii. these data strongly suggest that the trypsin-like protease in the mite is a der f iii allergen. | 1992 | 1444822 |
| [evaluation of oriton ige, a new kit for measurement of allergen specific ige antibodies]. | to determine whether oriton ige kit, a new kit for the measurement of allergen-specific ige antibodies, is useful in screening allergen-specific antibody, we measured the titers of ige antibodies against 11 different allergens (house dust 2, dermatophagoides farinae, japanese cedar, ragweed pollen, egg white, milk, cat epithelium, dog epithelium, candida, alternaria and aspergillus) with the oriton ige kit, and the results were compared to those of intradermal tests and rast in 103 allergic pati ... | 1992 | 1444828 |
| [evaluation of the sensitized condition of patients with allergic diseases in okinawa using the mast allergy system]. | we determined, using the mast system, specific ige antibodies to allergens in the circulating blood of 127 patients with bronchial asthma, allergic rhinitis and atopic dermatitis in okinawa. the positive rates to inhalant allergens in all patients examined by the mast system were as follows: dermatophagoides farinae 65%, house dust 58%, cat epithelium 17% and japanese cedar 9%. in addition, the positive rates to food allergens found in all patients were as follows: wheat 16%, shrimp 14%, egg whi ... | 1992 | 1444829 |
| lymphocyte transformation test for house dust mite in atopic dermatitis: relationship between mite antigens for type i and type iv allergy. | using a crude extract obtained from dermatophagoides farinae, and its four fractions (i, ii, iii and iv) partially purified by high-speed gel filtration chromatography, scratch tests and lymphocyte transformation tests (ltts) were performed on 37 patients with atopic dermatitis. crude mite antigen provoked positive scratch test reactions in 25 (68%), and positive ltt reactions in 19 (51%) of the 37 patients examined. mite antigen fractions i, ii, iii and iv induced a positive ltt reaction in 19, ... | 1992 | 1476036 |
| [involvement of allergic mechanism in minimal change nephrotic syndrome (mcns)]. | in this study we evaluated the involvement of allergic mechanisms in patients with adult onset mcns (17 cases) by measuring serum ige levels and rast scores to house dust 1 (h.d.1), house dust 2 (h.d.2), dermatophagoides farinae (d.f.), dermatophagoides ptenonyssinus (d.p.). out of the 17 cases, three cases showed high levels of ige (more than 1000 iu/ml) and the mean level of serum ige was 877 iu/ml before treatment. all cases were treated with steroid and/or cyclophosphamide. after the treatme ... | 1992 | 1482298 |
| prevalence of dust-mite allergens in homes and workplaces of the upper connecticut river valley of new england. | this study sought to determine the relative abundance and seasonal variation of dermatophagoides farinae and d pteronyssinus allergens in homes and workplaces of the upper connecticut river valley of new england. dust samples were obtained from 15 homes and 23 carpeted workplaces in public buildings. half the samples were collected in june 1990 and half in september 1990. d pteronyssinus and d farinae content of the samples were determined by enzyme-linked immunosorbent assay (elisa). this obser ... | 1992 | 1483576 |
| water balance and humidity requirements of house dust mites. | the house dust mites, dermatophagoides farinae, d. pteronyssinus and euroglyphus maynei, are prevalent in homes in humid geographical areas throughout the world. these mites thrive in humid environments in human dwellings where there is no liquid water to drink. however, their bodies contain 70-75% water by weight, which must be maintained in order to reproduce. their primary source of water is water vapor which is actively extracted from unsaturated air. at relative humidities above 65-70%, ade ... | 1992 | 1493744 |
| ultrastructure of the reproductive system of the house dust mites dermatophagoides farinae and d. pteronyssinus (acari, pyroglyphidae) with remarks on spermatogenesis and oogenesis. | several parts of the reproductive system of both sexes of dermatophagoides farinae and d. pteronyssinus are investigated and compared by light-, scanning electron-, and transmission electron microscopy. new techniques have been employed for scanning of the internal structures of these mites. the male reproductive system consists of unpaired testis, paired vasa deferentia, an accessory gland, ejaculatory duct, and copulatory organ. the female reproductive system consists of bursa copulatrix, duct ... | 1992 | 1493749 |
| [the antigenic and allergenic properties of an extract from the house dust mite dermatophagoides farinae]. | to evaluate the antigenic composition and allergenic activity of d. farinae extracts, the methods of rocket and cross immunoelectrophoresis, gel chromatography and the inhibition of the radioallergosorbent test have been used. the presence of 9-20 antigenic components has been established. the fractions of the extract with a molecular weight of 10-200 kd possessed the highest capacity of inhibiting the binding of specific ige antibodies from the pool of sera from patients sensitized to d. farina ... | 1992 | 1496879 |
| [bronchial asthma in children with sensitization to mites]. | overall 180 children with bronchial asthma induced by sensitization to dermatophagoides pteronyssinus and dermatophagoides farinae were examined clinically and immunologically. the patients' age ranged from 1 to 14 years. a relationship was established between bronchial asthma exacerbation and the stay at home. in that disease pattern, asphyxia attacks were noted to occur more frequently in the evening and night hours. emphasis is laid on the leading part played by the ige-mediated mechanism in ... | 1992 | 1513646 |
| prevalence of dust mites in the homes of people with asthma living in eight different geographic areas of the united states. | the density and species prevalence of dust mites were determined at various times over a 5-year-period in 252 homes of dust mite sensitive people with asthma who lived in eight geographic areas of the united states (cincinnati, ohio; new orleans, la.; memphis, tenn.; galveston, texas; greenville, n.c.; delray beach, fla.; san diego and los angeles, calif.). the most common dust mites found in the homes were dermatophagoides farinae (df), d. pteronyssinus (dp), euroglyphus maynei (em), and blomia ... | 1992 | 1527314 |
| early childhood risk factors for sensitization at school age. | early childhood risk factors for current sensitization were investigated by use of cross-sectional data of a longitudinal study in southwest germany. information was gathered by questionnaires from 1812 families of whom 1470 children 6 to 8 years old were tested by means of a skin prick test (spt) with seven aeroallergens. groups with sensitization (n = 201; positive spt to grass pollens 6.6%, dermatophagoides pteronyssinus 6.5%, dermatophagoides farinae 4.4%, cat dander 4.6%, any of the tested ... | 1992 | 1527318 |
| cd4+ cd45ra+ t cells modulate allergen-induced interleukin 2 responsiveness in human lymphocytes. | peripheral blood lymphocytes from nonallergic individuals acquired responsiveness to interleukin 2 (il2) after stimulation with ovalbumin (ova) or dermatophagoides farinae (df) antigens when they were pretreated with the cd45ra antibody, which has been shown to define the suppressor inducer subset of cd4+ cells and also to block its suppressor activity. the effect provided by the cd45ra antibody was lost if the lymphocytes had initially been activated with the ova of df antigens. the magnitude o ... | 1992 | 1531787 |
| [studies on igg subclass antibodies in adult asthma. 2. changes in serum antigen specific igg subclass antibodies for aging and intractability in asthmatics]. | to clarify the factors which induce intractable asthma, the level of serum igg subclass antibodies to mite (dermatophagoides farinae) and candida antigens (candida albicans) for aging and severity was investigated in 230 bronchial asthmatics (male: 117, female: 113) aged 6-81 years old (mean age = 40). total ige level and ige antibodies to mite and candida antigens were measured by radioimmunosorbent test (rist) and radioallergosorbent test (rast), respectively. the serum level of igg and igg1 a ... | 1992 | 1554328 |
| trimodality of serum dermatophagoides farinae-specific ige rast levels in atopic dermatitis patients. | serum dermatophagoides farinae-specific ige rast (df ige) levels were assayed in 122 atopic dermatitis patients at the first examination. from the statistical study, we found trimodality of the individual variability of serum df ige levels. we hypothesize that serum df ige levels may be controlled by a pair of allelic genes. | 1992 | 1582702 |
| [eosinophil colony stimulating factor production by lymphocytes from patients with bronchial asthma]. | to investigate the eosinophilia in patients with bronchial asthma (ba), we examined the release of eosinophil colony stimulating factor (eo-csf) from blood mononuclear cells (mnc) and lymphocytes from ba. we also investigated the effect of specific antigens on the release of eo-csf to determine its relation to other known eo-csfs. 1) phytohaemagglutinin (pha) and interleukin (il)-2 at optimal concentrations stimulated mononuclear cells from ba, and induced eo-csf release. in contrast, mnc from n ... | 1992 | 1625398 |
| the house dust mite dermatophagoides pteronyssinus is the most important allergen on the island of mauritius. | to determine the relative importance of mites as a cause of allergic sensitivity and asthma on the western indian ocean island of mauritius, we measured specific ige antibodies to common inhalant allergens in sera from mauritians claiming to have allergic symptoms and we examined house dust samples for evidence of mites and their allergens. seventy-two of the 110 sera tested (65%) contained detectable ige antibody to at least one mite, mould or pollen allergenic extract. by far the most prevalen ... | 1992 | 1628251 |
| reduction of environmental mites improved atopic dermatitis patients with positive mite-patch tests. | during a series of studies on the involvement of house dust mite antigens in 183 cases of atopic dermatitis, we observed an improvement in two patients following the removal of mites from their environment by means of a thorough housecleaning and replacement of the mattress. both patients manifested the typical clinical skin lesions of atopic dermatitis and had similar laboratory findings. although the serum ige concentrations and specific ige to dermatophagoides pteronyssinus and dermatophagoid ... | 1992 | 1640023 |
| skin cross-reactivity between dermatophagoides pteronyssinus and storage mites in atopic children. | in our environment, some children with allergic respiratory pathology do not have as favorable an evolution as expected after three years of immunotherapy with d. pteronyssinus extracts. we believe this is due to the existence of other mites whose antigenic determinants are not present in d. pteronyssinus. the purpose of the present study was to evaluate skin cross-reactivity between dermatophagoides and storage mites in children with bronchial asthma. fifty patients were chosen, with a mean age ... | 1991 | 1669575 |
| ovalbumin-specific induction of il2 responsiveness in lymphocytes from patients with hen egg allergy and its regulation by the culture supernatant of normal lymphocytes. | interleukin 2 (il2) responsiveness of ovalbumin (ova)-stimulated lymphocytes from patients with hen-egg allergy was studied. the number of viable cells of 5-day cultured lymphocytes stimulated with ova was increased by an additional three days incubation with recombinant il2. this phenomenon was not observed when the lymphocytes of patients allergic to ova but not to dermatophagoides farinae extract antigen (df) were stimulated with df. normal lymphocytes stimulated with ova expressed tac antige ... | 1990 | 1688691 |
| basophil histamine release and airway response to mite allergen in atopic dermatitis. | twelve patients with atopic dermatitis (ad) were subjected to in vitro histamine release from peripheral blood leukocytes (basophils) and in vivo bronchial inhalation challenge using house dust mite (dermatophagoides farinae) allergen. not only seven patients with asthmatic history but also five patients without asthma responded to both the in vitro and the in vivo challenges. a significant correlation was observed between hr30 (a mite concentration producing a 30% release of total cellular hist ... | 1990 | 1699458 |
| [histamine release test of patients allergic to the house dust mite, dermatophagoides farinae--analysis according to various clinical backgrounds]. | since the histamine release test is more complicated than some of the other tests, there are smaller number of clinical studies employing this method. in this study, we selected 25 males and 20 females, allergic to the house dust mite, to undergo the histamine release test challenged by 7 serial concentrations of the mite allergen diluted from 10 times to 10 million times the original allergen at a concentration of 0.01%. histamine release was expressed as a percentage of the total histamine con ... | 1991 | 1711309 |
| the distribution of cat and dust mite allergens on wall surfaces. | this study was undertaken to determine the distribution of cat and dust mite allergens on wall surfaces and to assess the value of wall-wipe samples as a measure of allergen exposure. paired samples were collected from 31 homes, 20 homes with cats and 11 homes without, by vacuuming 1 m2 of carpet and by wiping 1 square foot of an adjacent wall. felis domesticus allergen i (fel d i) was detected in 30 of 31 settled dust samples (range, not detectable to 832,000 mu/gm; median, 10,250 mu/gm) and in ... | 1992 | 1730832 |
| sensitivity to common allergens: relation to respiratory symptoms and bronchial hyper-responsiveness in children from three different climatic areas of australia. | in order to examine further the relation between atopy, as defined by skin-prick tests, and respiratory illness, we studied three populations of schoolchildren aged 8-11 years and living in different climatic areas of new south wales, australia. skin-prick tests were performed using 13 commercial allergen extracts. respiratory and allergic symptoms were assessed using a self-administered questionnaire to parents and bronchial hyper-responsiveness (bhr) was measured by histamine inhalation test. ... | 1991 | 1742649 |
| sensitization to blomia tropicalis and lepidoglyphus destructor in dermatophagoides spp-allergic individuals. | serum was collected from 77 atopic subjects from cartagena, colombia, who had a positive skin test (greater than or equal to 3 mm) to either dermatophagoides farinae (df) (73) or d. pteronyssinus (dp) (70). twenty-nine healthy volunteers with negative allergy histories and negative skin tests were used as control subjects. rast and rast-inhibition assays were used to study the prevalence of specific ige to blomia tropicalis (bt), lepidoglyphus destructor (ld), and df. rast results were considere ... | 1991 | 1744365 |
| [effect of intensive vacuum cleaning in reducing house dust mite antigen in bed rooms of asthmatic children]. | house dust mite (dermatophagoides farinae) antigen was measured by enzyme-linked immunosorbent assay in house dust samples obtained from the bed rooms of asthmatic children and normal controls. in 37 rooms where regular vacuum cleaning was performed, the mean of d.f. antigen was 4.42 +/- 7.55 micrograms/m2. in the 24 bed rooms of asthmatic children, mean levels of d.f. antigen decreased significantly (p less than 0.05) after intensive cleaning (systematic cleaning) was continued for 4 weeks (0.7 ... | 1991 | 1747518 |
| [analysis of clinical factors and ige-rast of dermatophagoides farinae and rice in atopic dermatitis by multiple factor analysis of quantification theory type ii]. | twelve clinical factors and ige-rast of dermatophagoides farinae (df) and rice were analysed in three hundred patients with atopic dermatitis by multiple factor analysis of quantification theory type ii. atopic dermatitis was simply defined here, as a patient who had typical eczematous eruption on the flexural portions of the body, such as the cubital fossa and/or the tibial fossa and/or the neck. the results were as follows: 1) five clinical items such as onset age, age, history of asthma, sex, ... | 1991 | 1793369 |
| isolation of cysteine protease in the crude mite extract, dermatophagoides farinae. | in order to study the relationship between cysteine protease and der f i, which is one of the major allergens in the mite, dermatophagoides farinae, isolation of cysteine protease was attempted using various column chromatographies. both the potent cysteine protease activity and the allergenic activity were detected in the same fractions by anion exchange chromatography on a deae-sephacel, gel chromatographies and chelating sepharose 6b chromatography. in the double immunodiffusion test, the fin ... | 1991 | 1804791 |
| studies of dialysates from dermatophagoides farinae: partial purification and haptenic properties of dialysates from dermatophagoides farinae. | dialysates from dermatophagoides farinae were partially purified. fractionation on hplc and anion exchange chromatography revealed that the dialysates consisted of 5 major fractions of glycoprotein whose apparent molecular sizes were 5.1, 4.1, 3.2, and less than 1.35 kd on hplc. the apparent molecular size of two fractions was 5.3 and 2.9 kd on sds-page. they were basic glycoproteins which had a pi ranging from 7.46 to 8.71 on pag-ief. these fractions were allergenic in the rast and elisa inhibi ... | 1991 | 1809689 |
| ciliary activity in the tubotympanum during type i allergic reaction. | the immune system is active in the tubotympanum and some middle ear diseases might be partially or exclusively immunologically mediated. however, the effect of an allergic reaction on the tubotympanic cilia remains to be elucidated. the response of normal tubotympanic cilia from the guinea pig was investigated in an in vitro experimental system to evaluate ciliary responsiveness without the influence of secretions. mucosal samples were obtained from three different tubotympanic locations (eustac ... | 1991 | 1842864 |
| rast in diagnosis and therapy of allergic rhinitis. | skin tests, rast, nasal provocation tests, and other nasal allergy examinations were performed on 463 patients exhibiting symptoms of allergic rhinitis who were examined at the department of otolaryngology of osaka city university medical school, and comparisons were made between the rast scores and the results of the other examinations. a study was also made of the change in grade of the allergic rhinitis and the change of the rast score resulting from one year of immunotherapy. the rates of ag ... | 1991 | 1842868 |
| identification of major allergens from the house dust mites, dermatophagoides farinae and dermatophagoides pteronyssinus, by electroblotting. | the allergens were separated from the extracts of house dust mites by sds-polyacrylamide gel electrophoresis (sds-page) and identified by autoradiography. over 30 protein bands of the whole body extract of dermatophagoides farinae were apparent on 10-20% gradient sds-page, and 13 bands with mw between 93kd and 12kd bound with specific ige antibodies in patients' sera sensitive to house dust mites. the major allergenic component of the whole body extract of d. farinae was the protein of mw 14-15k ... | 1991 | 1877252 |
| diagnosis of nasal allergy to the house dust mite. | twenty-five patients with perennial rhinitis and a positive skin prick test (spt) for dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) were submitted to nasal provocation and the radioimmunosorbent test (rast) for specific ige-antibodies. we found a significant correlation in the reaction to both allergen extracts for all parameters examined. in addition, there was a significant correlation among the spt, the rast and the nasal provocation for dp and between the spt and the ... | 1991 | 1891679 |
| prevalence of specific ige to the storage mite, aleuroglyphus ovatus. | aleuroglyphus ovatus (ao) is a storage mite that has a worldwide distribution and has been found in stored bran, wheat, chicken meal, and dried fish products. ao was isolated from a chicken meal sample, cultured on whole wheat flour at 75% relative humidity, and 1 gm of pure ao bodies were extracted, 1:20 wt/vol, in 0.2 mol/l of ammonium bicarbonate. der p i, major allergen of dermatophagoides, pteronyssinus (dp), or der f i major allergen to dermatophagoides farinae (df), was not detected in ex ... | 1991 | 1918725 |
| synthesis of biologically active recombinant der f ii. | a cdna library corresponding to mite protein was screened employing anti-der f ii antibody. two possible clones containing plasmids pfl1 and pfl11 were obtained. the two plasmids had insertions of about 500 basepairs. the dna sequences of the two insertions were determined, from which the amino acid sequences were deduced. the amino acid sequence of the purified native der f ii protein could be determined to 45 residues from the n terminus. as a result of comparison, we concluded that the cdnas ... | 1991 | 1937898 |
| preparation of specific monoclonal antibodies against der f ii one of the major allergens of dermatophagoides farinae. | five monoclonal antibodies (mabs) against der f ii, one of the major allergens of dermatophagoides farinae, were obtained from mice immunized with the allergen. western blotting analysis revealed that only one mab, named 15e11, was specific for der f ii, whereas the other mabs crossreacted with der p ii, one of the major allergens of dermatophagoides pteronyssinus. these results indicate the possibility that the der f ii-specific mab, 15e11, is a useful reagent for specific determination of der ... | 1991 | 1958138 |
| house dust mites in the west indies. | the purpose of this study was to determine the role of the house dust mite. dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df), in allergic diseases on the island of st. lucia. dust samples were taken from living quarters of patients and hospital volunteers. the level of df antigen in these living quarters was measured by rast inhibition. all samples showed df antigen within a large range of values. prick tests were done to house dust mite and molds on 91 patients with suspect ... | 1991 | 2014932 |
| sequence analysis of cdna coding for a major house dust mite allergen, der f i. | a cdna clone coding for der f i, a major allergen from the house dust mite dermatophagoides farinae has been isolated and sequenced. it codes for a putative 18-residue signal peptide, an 80-residue proenzyme region, and a 223-residue mature protein with a derived molecular weight of 25,191. the deduced amino-acid sequence shows significant homology to other cysteine proteases in the proregion as well as in the mature protein. sequence alignment of the mature der f i protein with the homologous a ... | 1991 | 2021874 |
| cdna encoding the major mite allergen der f ii. | cdna encoding the major house dust mite allergen der f ii from dermatophagoides farinae was amplified using the polymerase chain reaction and cloned into e. coli. it encoded a 129-residue protein with a calculated molecular weight of 14,021 d and had the expected high homology (88%) with der p ii including the absence of n-glycosylation sites and conserved cysteine residues. these results are consistent with the high degree of antibody crossreactivity and may help identify the differences in t-c ... | 1991 | 2021876 |
| dermatophagoides farinae allergen levels from two different sources within the same home: evaluation of two different collection techniques. | the allergens produced by the dust mites of the dermatophagoides genus are now recognized as one of the major allergens in house dust. quantification of those allergens helps to evaluate the effectiveness of avoidance measures and specific treatments aimed at the reduction of the mites and their allergens. the present study was undertaken to compare the level of d. farinae allergen collected by a standard vacuum cleaner with a disposable bag and the level of allergen collected by a hand-held vac ... | 1991 | 2035907 |
| triggering of the vascular permeability reaction by activation of the hageman factor-prekallikrein system by house dust mite proteinase. | a 30-kilodalton (kda) proteinase from the house dust mite dermatophagoides farinae (df-proteinase) was recently purified (takahashi et al. (1990) int. arch. allergy appl. immunol. 91, 80-85). in this paper we detailed the biological activities of the df-proteinase. the activation of the kinin cascade by df-proteinase was examined in vitro by using purified guinea pig hageman factor (hf), prekallikrein (pk) and high-molecular-weight kininogen (hmwk) and the effect of this proteinase on endogenous ... | 1991 | 2043681 |
| house-dust-mite allergens: a review. | the house-dust mites, dermatophagoides farinae, d. pheronyssinus and euroglyphus maynei are cosmopolitan inhabitants of the homes of humans worldwide. these mites are the sources of multiple potent allergens that trigger allergic reactions in house-dust-mite-sensitive individuals. many laboratories using widely varied mite materials and allergic sera, and biochemical and immunological assays, have isolated and characterized, to varying degrees, some of the allergens produced by these mites. the ... | 1991 | 2044430 |
| [eosinophil chemotactic activity in the supernatant of mononuclear cell culture stimulated with specific antigen]. | peripheral blood mononuclear cells (pbmc) separated from patients with asthma who were sensitive to dermatophagoides farinae (df) were cultured in alpha-medium for 5 days at 37 degrees c in 5% co2, in the presence or absence of 10 ng/ml of df antigen. eosinophils were purified from the peripheral blood of patients with eosinophilia who were not sensitive to df. eosinophil chemotactic activity (eca) was tested using a modified boyden chamber method. eca in the supernatant of pbmc stimulated with ... | 1991 | 2069510 |
| [suppressive effect of saibokuto on dermatophagoides farinae (df) antigen-induced il2 responsiveness of lymphocytes from asthmatic children]. | the effect of saibokuto on induction of antigen specific il2 responsiveness was studied. saibokuto suppressed the induction of il2 responsiveness induced by dermatophagoides farinae (df) antigen in peripheral blood mononuclear cells from patients with bronchial asthma. saibokuto-pretreated adherent cells failed to present df antigen to non-adherent responding t cells for induction of their il2 responsiveness. the same dose of saibokuto had no impact on non-adherent cells. saibokuto also suppress ... | 1990 | 2073141 |
| [comparison of the cutaneous response to dermatophagoides farinae from 2 separate laboratories]. | we compared in 50 asthmatic children, the cutaneous response by skin prick test to dermatophagoides farinae with antigens from two different laboratories (freeman and hollister). in order to evaluate the importance of the selection, conservation and standardization of the allergic extracts. 27/50 children had a similar response, in those cases (6) where freeman was doubtful and hollister positive the allergic etiology was confirmed by rast and challenge tests. in 5 cases, freeman was negative an ... | 1990 | 2089605 |
| [anti-cd45r antibody-treated normal lymphocytes respond to interleukin 2 (il2) after stimulation by allergens]. | interleukin 2 (il2) responsiveness was specifically induced by dermatophagoides farinae (df) antigen in df-sensitized lymphocytes from asthmatic children, but not in normal lymphocytes. df-induced il2 responsiveness was also observed in normal lymphocytes pretreated (day 0) with anti-cd45r antibody, which recognize suppressor inducer subset among cd4+ t cells. however anti-cd45r antibody was no longer effective when the lymphocytes were cultured for more than one day with the antigen, suggesting ... | 1990 | 2140934 |
| cloning and sequencing of cdnas corresponding to mite major allergen der f ii. | a cdna library corresponding to mite protein was screened employing anti-der f ii antibody. two possible clones were obtained, which contained plasmids, pfl1 and pfl11, respectively. both plasmids had insertions of about 500 base pairs. the dna sequences of the two insertions were determined, from which the amino acid sequences were deduced. the amino acid sequence of the purified native der f ii protein could be determined to 45 residues from the n-terminus. as a result of comparison, we conclu ... | 1990 | 2222199 |
| effects of five insect growth regulators on laboratory populations of the north american house-dust mite, dermatophagoides farinae. | the potential of insect growth regulators (methoprene, hydroprene, fenoxycarb, diflubenzuron and triflumuron) to control populations of the north american house-dust mite dermatophagoides farinae (hughes) was assessed in laboratory bioassays. methoprene was most effective at suppressing population growth, especially at concentrations of 1.0% (10,000 ppm) and 5.0% (5000 ppm) active ingredient. hydroprene, structurally related to methoprene, also suppressed house-dust mite populations but not as c ... | 1990 | 2226070 |
| the control of house dust mite allergens in rugs. | used rugs were vacuum cleaned, "wet cleaned," "shampooed," or heated in an autoclave. the effects on the live-mite population, on the allergen (dermatophagoides pteronyssinus, der p i and der p ii; and dermatophagoides farinae, der f ii) content, and on the suitability of the rugs as a habitat for house dust mites was studied. autoclaving was most effective in all three aspects. the cleaning methods were quite inefficient for killing the mites, but a significant reduction of allergens and deteri ... | 1990 | 2229844 |
| antigenic analysis of group i house dust mite allergens using random fragments of der p i expressed by recombinant dna libraries. | antigenic regions of a major house dust mite allergen, der p i, were identified by a recombinant dna strategy employing the technique of random fragmentation. fragments of cdna coding for der p i were produced by sonication and used to construct lambda gt 11 expression libraries. analyses of recombinant fragments reactive with a rabbit anti-der p i antiserum showed that the b cell determinants expressed in escherichia coli were limited, with the majority (86%) of antigenic clones isolated mappin ... | 1990 | 2246073 |
| relationship between asthma and seasonal allergic rhinitis in schoolchildren. | the relationship between sensitization to common allergens and the development of asthma and seasonal allergic rhinitis (sar) was investigated in a total of 419 schoolchildren in a farming community where allergens of both dermatophagoides farinae (df) and orchard grass (og) pollen predominate. twelve subjects (2.9%) were found to have asthma and 35 subjects (8.4%) sar. there was no significant association between occurrences of asthma and sar. the incidence of asthma correlated with df-specific ... | 1990 | 2256580 |
| [the standard level of ige rist and eosinophil counts in 0-year old allergic children according to multiple factor analysis type ii]. | we specified three standard levels of ige rist (10, 20, 60 iu/ml) for the ninety-four (94) allergic clients of 0-year infants, who classified with three groups. each group was furthermore divided in two groups such as a group under the standard level and a group exceeding the level respectively. next, using the multiple factor analysis type ii, we examined quantitative analysis that each of three classified groups was influenced on 12 allergic factors of sex, existence and inexistence of wheezin ... | 1990 | 2260928 |
| [results of patch test using mite components in atopic dermatitis (ad) patients. 1st report. "as is patch test" using crushed mites]. | as is patch test using crushed live mites and crushed dried dead mites of dermatophagoides pteronyssinus (d.p.), dermatophagoides farinae (d.f.), & tryophagus ptrescentiae (t.p.) was performed on 49 ad patients and 18 control patients suffering from dermatitis other than ad. results: 1) with crushed dried dead mites all patients showed negative reactions but with live mites 11 among 49 ad patients were positive (22.4%); 2) female mites were more positive than males at the rate 8:1; 3) with cases ... | 1990 | 2273575 |
| enzyme-linked immunosorbent assay for quantifying antigens of dermatophagoides farinae and d. pteronyssinus (acari: pyroglyphidae) in house dust samples. | double-antibody sandwich enzyme-linked immunosorbent assay (elisa) using a monoclonal antibody as capture and detector antibodies was developed for quantifying antigens of dermatophagoides farinae hughes and d. pteronyssinus (trouessart) mites contained in house dust samples. this monoclonal antibody was directed against mite antigens that were also reactive to immunoglobulin e antibody in all of 10 serum samples obtained from patients allergic to mites. histological study using fluorescent anti ... | 1990 | 2280398 |
| [suppressive effect of azelastine on the induction of df antigen specific il-2 responsiveness in lymphocytes from patients with bronchial asthma]. | effect of azelastine on the induction of dermatophagoides farinae (df)-antigen specific il-2 responsiveness of the lymphocytes was examined. azelastine suppressed the induction of il-2 responsiveness induced by df antigen in peripheral blood mononuclear cells from patients with bronchial asthma in a dose dependent manner. azelastine suppressed antigen presenting adherent cells but not non-adherent responder cells (t cell rich fraction). the antigen specific il-2 responsiveness induced by purifie ... | 1990 | 2288496 |
| house dust mite allergy in florida. mite survey in households of mite-sensitive individuals in tampa, florida. | this study evaluated the prevalence of positive house dust mite skin tests in a population of atopic individuals and identified the mite species present in mattress and house dust samples in homes of the tampa bay area. four hundred consecutive individuals were evaluated for respiratory complaints and skin tested with standardized extracts of dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df). two hundred forty individuals (60%) had a positive skin test to the mite extracts. d ... | 1990 | 2292372 |
| immunoblot analysis of dermatophagoides farinae antigen. | we isolated the allergenic components of dermatophagoides farinae (d. farinae) body extracts and determined the frequencies and specificities of ige, igg, and igg4 antibodies binding to components of d. farinae extracts by use of immunoblot technique. a total of 29 ige-binding components of d. farinae extracts were identified and distributed over the molecular weight range of 13 to 140 kilodaltons (kd). each component had 3% to 73% frequencies of recognition by individual patient serum, and the ... | 1990 | 2301783 |