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oxygen consumption of the blue crab, callinectes sapidus rathbun, from proecdysis to postecdysis. 19763345
identification of species in cooked crabmeat by thin layer isoelectric focusing.thin layer polyacrylamide gel isoelectric focusing (tlief) is described for characterizing the species-specific, heat-denatured proteins of 8 species of crab: red (geryon quinquedens), rock (cancer irroratus), jonah (cancer borealis), blue (callinectes sapidus), king (paralithodes camtschatica), snow (chionoectes spp.), european edible (cancer pagurus), and dungeness (cancer magister). protein pattern differences are shown not only among species, but also between 2 modes of heat processing of th ...197939059
response of isolated axonal preparations to hyperosmoticity: evidence for a cellular volume regulation.when submitted to a hyperosmotic stress, isolated axons of callinectes sapidus shrink. volume regulation is observed if the experiment is carried out by using serum from a crab adapted to sea water. 3' : 5'-amp and dibutyryl amp are also effective. a compound isolated from the hemolymph and having a molecular weight close to 10 000 mimics the effects of the serum and induces a partial regulation of the axonal volume.197665947
the function of hemocyanin in respiration of the blue crab callinectes sapidus.blood po2 in the blue crab callinectes sapidus, a very active species of tropical origin, is lower at 22 degrees c than that of larger crabs in colder waters. these low oxygen levels permit its hemocyanin to be highly oxygenated at the gill, and to deliver almost half of its oxygen to the tissues in resting animals. sustained muscular activity results in conspicuous decreases in blood po2, ph and hemocyanin oxygenation. although the venous reserve is fully utilized, hemocyanin oxygenation at the ...1975240904
the influence of environmental salinity on hemocyanin function in the blue crab, callinectes sapidus.the effects of inorganic ions and of the hydrogen ion on oxygen-binding properties of most respiratory pigments are opposite. the addition of salt to the medium increases oxygen affinities, and the addition of h+ decreases oxygen affinities of crustacean hemocyanins. these oxygenation properties, as observed in vitro, suggest that the oxygen-transport system must adapt to ionic changes in the blood. in fact, decreases in the salt concentration of the blood of estaurine blue crabs are accompanied ...1975240905
evaluation of millipore coli-count samplers for monitoring fecal coliforms in the blue crab, callinectes sapidus.analyses of crab meat homogenates with coli-count samplers (millipore corp.) resulted in fecal coliform counts comparable to counts obtained from standard most-probable-number testing procedures.1979391154
the influence of salinity on toxicity of cadmium and chromium to the blue crab, callinectes sapidus. 1979444712
chlorine, temperature, and exposure duration effects of power plant effluents on juvenile blue crabs callinectes sapidus and grass shrimp palaemonetes pugio. 1979490683
effects of mercuric chloride on the resting membrane potentials of blue crab (callinectes sapidus) muscle fibers.the effects of concentrations of mercuric chloride ranging from 3.68 to 368 microm (1--100 ppm) on the resting membrane potentials of muscle fibers in the extensor muscle of the carpopodite in walking legs of adults blue crabs were investigated in vitro. glass micropipettes filled with saturated solutions of potassium chloride and conventional electronic equipment were used. resting membrane potentials in uncontaminated artificial sea water averaged -78 mv +/- 4 mv (s.e.m.). mercuric chloride ad ...1979473223
bacterial flora of the hemolymph of the blue crab, callinectes sapidus: most probable numbers.the hemolymph of 290 freshly collected blue crabs from chincoteague bay, va., was sampled over a 15-month period from august 1968 through november 1969 and most probable numbers of bacteria were determined by tube dilution. the hemolymph of 18% of all crabs sampled was found to be sterile, with 16% sterility in summer and 23% in winter samples. despite individual variations, male crabs as a group had a higher bacterial hemolymph burden than females, and among both sexes summer counts were higher ...19751090257
bacterial flora of the hemolymph of the blue crab, callinectes sapidus: numerical taxonomy.bacteria isolated from the hemolymph of normal blue crabs were found to be predominantly vibrio sp., pseudomonas sp., acinetobacter sp., bacillus sp., flavobacterium sp., and coliforms. vibrio parahaemolyticus, a cosmopolitan facultative pathogen widely implicated in outbreaks of gastroenteritis related to the consumption of improperly processed seafood, was present in crabs collected between the months of may to november and was identified in up to 21% of the hemolymphs sampled.19751090258
changes in ecdysteroids during embryogenesis of the blue crab, callinectes sapidus rathbun. 1979437355
ionic and osmotic regulation and metabolic response to salinity of juvenile callinectes sapidus rathbun. 1975241556
free amino acid changes in normal and eyestalkless megalopa larvae of the blue crab, callinectes sapidus, during the course of the molt cycle. 1975236891
volume regulation and alanine transport. response of isolated axons of callinectes sapidus rathbun to hypo-osmotic conditions. 1975236878
temperature effects on axonal conduction in callinectes sapidus (rathbun) and carcinus maenas (l.). 1975236872
shell disease and metal content of blue crabs, callinectes sapidus, from the albemarle-pamlico estuarine system, north carolina.concentrations of 13 elements were determined for three tissues (gill, hepatopancreas, muscle) in diseased crabs from a contaminated estuary (pamlico river, nc), and in non-diseased crabs from both the contaminated estuary and a relatively uncontaminated area (albemarle sound, nc) during the fall 1989 and summer 1990. the diseased crabs had lesions which completely penetrated their dorsal integument, while the non-diseased crabs lacked lesions. sediments within the contaminated area showed enric ...19921456781
variations in serum constituents of the blue crab, callinectes sapidus: copper and zinc. 1975234043
recognition of viruses and xenogeneic proteins by the blue crab, callinectes sapidus. i. clearance and organ concentration. 1977205450
cytological observations on the blood and hemopoietic tissue in the crab, callinectes sapidus. i. the fine structure of hemocytes from intermolt animals.a fine structural analysis of hemocytes recovered from the pericardial sinus of the blue crab (callinectes sapidus) demonstrates that hyaline cells, intermediate cells, and granulocytes are present in the hemolymph. a detailed consideration of their shared and differing cytological features suggests that the various blood cells represent different stages of cytogenesis along a common or single path of cellular differentiation. the potential functional complexity of the hemocytes is attested to b ...1978204416
calcitonin-like immunoreactivity in the blue crab: tissue distribution, variations in the molt cycle, and partial characterization.calcitonin-like immunoreactivity has been found in blood and tissues of the marine blue crab, callinectes sapidus rathbun. the activity in blood rises significantly during the immediate premolt (d4) stage, but tissue immunoreactivity does not vary significantly with molt stage. the immunoreactivity to antisalmon calcitonin serum is due to a 27.2 kda protein that shows strong similarities in amino acid composition to a similar protein in a lobster and to human calcitonin precursor. the protein is ...19921640198
temperature and blood acid-base status in the blue crab, callinectes sapidus.the blood acid-base status of blue crabs. callinectes sapidus, was assessed at temperatures ranging from 10 to 30 degrees c. blood ph varied inversely with temperature, with a slope (dph/tt) of - 0.012. the paco rose only slightly between 10 and 27 degrees c, from 2.09 torr to 2.91 torr, with the result that the total dissolved co2 actually fell slightly due to the decrease in solubility. in vitro the blood behaved like a conventional rosenthal system, with a rise in pco2 at constant ct which wa ...197833428
characterization of glutathione s-transferase activity in tissues of the blue crab, callinectes sapidus. 197830574
the effects of pentachlorophenol (pcp) and 2,4-dinitrophenol (dnp) on the oxygen consumption of tissues from the blue crab, callinectes sapidus, under different osmotic conditions. 197828895
the subunit characterization of callinectes sapidus hemocyanin.hemocyanin from the blue crab, callinectes sapidus, sediments at 25.7 s and has a native molecular weight of 940 000 +/- 20 000. under solution conditions of increased ph (approximately 10) or ionic strength, the native molecule dissociates to a 17 s species. reversal of this dissociation was unsuccessful. at ph 10 and with the removal of mg2+, the 17 s species reversibly dissociates to form a subunit species which sediments at 6 s. a comparison of the circular dichroic spectra of the 25.7 s and ...197714702
respiration rates and atp concentrations in the excised gills of the blue crab as a function of salinity. 19751194
atomic structure of intracellular amorphous calcium phosphate deposits.the radial distribution function calculated from x-ray diffraction of mineralized cytoplasmic structures isolated from the hepatopancreas of the blue crab (callinectes sapidus) is very similar to that previously found for synthetic amorphous calcium phosphate. both types of mineral apparently have only short-range atomic order, represented as a neutral ion cluster of about 10 a in longest dimension, whose probable composition is expressed by the formula ca9(po4)6. the minor differences observed ...19751056015
comparative studies of intra- and extramitochondrial calcium phosphates from the hepatopancreas of the blue crab (callinectes sapidus).subcellular fractions of cytoplasmic mineral granules and mineral-loaded mitochondria were isolated from whole homogenates of hepatopancreas of the blue crab (callinectes sapidus). chemical, physical, and morphologic studies were carried out on both subcellular fractions, with and without prior removal of organic components by hydrazine extraction. in contrast to cytoplasmic granules, whole mitochondria contained appreciable amounts of mineral ions not associated with a solid mineral phase. quan ...1976990969
cheliped laterality in callinectes sapidus (crustacea: portunidae). 1976953070
comparative properties of channel catfish (ictalurus punctatus) and blue crab (callinectes sapidus) acetylcholinesterases.1. acetylcholinesterases (aches) from channel catfish and blue crabs were examined for substrate preference, kms, effects of inhibitors, and ph and osmotic activity profiles. 2. similarities were noted for substrate preference along with ph and osmotic optima. 3. crab ache had a lower km (9 x 10(-5) vs 2 x 10(-4) m) and was more sensitive in terms of ki50s than fish ache to eserine (2.6 x 10(-7) vs 3 x 10(-7) m), malathion (4.5 x 10(-5) vs 1.6 x 10(-4) m) and parathion (6.9 x 10(-5) vs 7 x 10(-4 ...19882905945
fate of labeled n-alkanes in the blue crab and stripped mullet.results of these studies demonstrate that the blue crab was readily able to take up and discharge labeled n-alkanes, but it was not able to metabolize them. in contrast, it appears that n-alkanes taken up via the digestive tract in mullet are readily metabolized, probably via microorganisms in the gut. more limited metabolism was observed in mullet when n-alkanes were taken up via the gills.1977861400
excitatory synapses of blue crab gastric mill muscles.physiological and ultrastructural studies were made of neuromuscular synapses in stomach muscles, especially two gastric mill muscles of the blue crab innervated by neurons of the stomatogastric ganglion. these muscles depolarized and contracted with application of glutamate, but not acetylcholine, whereas the dorsal dilator muscles of the pyloric region depolarized and contracted in acetylcholine, but not in glutamate. large excitatory postsynaptic potentials (epsp's) of 5-20 mv were recorded i ...1977837405
quaternary ammonium sulfanilamide: a membrane-impermeant carbonic anhydrase inhibitor.a novel carbonic anhydrase (ca) inhibitor, quaternary ammonium sulfanilamide (qas), was tested for potency as a ca inhibitor and for its ability to be excluded from permeating biological membranes. inhibitor titration plots of qas vs. pure bovine ca ii and ca from the gills of the blue crab, callinectes sapidus, yielded ki values of approximately 15 microm; thus qas is a relatively weak but effective ca inhibitor. permeability of the qas was directly tested by two independent methods. the inhibi ...19873107405
membrane-associated carbonic anhydrase in gills of the blue crab, callinectes sapidus.the presence of the enzyme carbonic anhydrase (ca) on the basal membrane of the branchial endothelial cells in the blue crab and its physiological significance were studied in vivo using a membrane-impermeant ca inhibitor, quaternary ammonium sulfanilamide (qas). injection of qas into the hemolymph of callinectes sapidus resulted in the rapid development of a respiratory acidosis; pco2 rose almost 2 torr, ph was lowered by approximately 0.25 units, and total co2 rose by 2 mm. these results suppo ...19873107406
occurrence of trematodes in nerves of the blue crab, callinectes sapidus. 19883373008
a minchinia-like haplosporidan parasitizing blue crabs, callinectes sapidus. 1976822104
a simple method for the computation of first neighbour frequencies of dnas from cd spectra.a procedure for the computation of the first neighbour frequencies of dna's is presented. this procedure is based on the first neighbour approximation of gray and tinoco. we show that the knowledge of all the ten elementary cd signals attached to the ten double stranded first neighbour configurations is not necessary. one can obtain the ten frequencies of an unknown dna with the use of eight elementary cd signals corresponding to eight linearly independent polymer sequences. these signals can be ...1978673843
an evaluation of acute bromine chloride toxicity to grass shrimp (palaemonetes spp.) and juvenile blue crabs (callinectes sapidus). 1978638269
nonhomogeneous excitatory synapses of a crab stomach muscle.neuromuscular synapses of pyloric muscle p1 in the blue crab callinectes sapidus were examined using electrophysiological and electron microscopic methods. the muscle is innervated by a single excitatory axon of the stomatogastric ganglion. excitatory postsynaptic potentials show striking facilitation at very low frequencies of stimulation, indicating very slow decay of the facilitation process after a single nerve impulse. quantal content of transmitter release at a low frequency of stimulation ...1978632814
total mercury and its distribution in blue crab and deep water pink shrimp from alexandria coast.total mercury content and its distribution in muscles and viscera of male and female blue crab (callinectes sapidus rothbum) and deep water pink shrimp (parapenacus longirostris) collected from the 3 main fishing grounds near the alexandria coast in the mediterranean sea was estimated. the results indicate that the mercury content in the muscles of both species differ according to fishing areas, size, sex, and species. more than 75% of total mercury were accumulated in the viscera of both specie ...19873696196
ponasterone a: a new ecdysteroid from the embryos and serum of brachyuran crustaceans.the apolar ecdysteroid present in the developing embryo of the blue crab, callinectes sapidus rathbun, is tentatively identified as ponasterone a (2 beta, 14 alpha, 20,22-pentahydroxy-5, beta-cholest-7-en-6-one) on the basis of chromatographic, immunological, and mass spectral evidence. the apolar ecdysteroid present in the serum of land crabs, gecarcinus lateralis, in the late premolt stages of the intermolt cycle is also tentatively identified as ponasterone a on the basis of chromatographic a ...1979538780
a disease of the blue crab, callinectes sapidus, of possible viral etiology. 19751151102
transverse sarcomere splitting. a possible means of longitudinal growth in crab muscles.transversely split sarcomeres are seen in mouthpart muscles of the blue crab in the electron microscope. sarcomeres split only at the h zone. two new sarcomeres are formed by a z disk which appears in the h zone of the splitting sarcomere. splitting may involve breaking of the thick filaments in the h zone, elongation of these filaments, and formation of both new actin filaments and z-disk materials, sarcomere splitting would allow longitudinal growth of muscle cells without lengthening of sarco ...1979457766
tonic immobility in the blue crab (callinectes sapidus, rathbun) its relation to threat of predation.the tonic immobility. response in the blue crab (callinectes sapidus, rathbun) was investigated in a series of seven experiments. although reported to be a powerful variable in other species, preinduction electric shock produced inconsistent increases in the duration of tonic immobility with the blue crab; manipulations that were more directly relevant to the fear of predation had considerably greater effects than shock. physical damage to the chelipeds, mirror image stimulation, and immobilizat ...19751171124
source of vibrio spp. found in the hemolymph of the blue crab, callinectes sapidus. 19854031533
seasonal effects of leached mirex on selected estuarine animals.four 28-day seasonal experiments were conducted using selected estuarine animals in outdoor tanks that received continuous flow of mirex-laden water. mirex (dodecachlorooctahydro-1,3,4-metheno-2h-cyclobuta [cd] pentalene) leached from fire ant bait (0.3% mirex) by fresh water and then mixed with salt water was toxic to blue crabs (callinectes sapidus), pink shrimp (penaeus duorarum), and grass shrimp (palaemonetes pugio) but not to sheepshead minnows (cyprinodon variegatus), at concentrations le ...19751190846
gas-bubble disease in the blue crab, callinectes sapidus. 19761254984
blue crab mortality: interaction of temperature and ddt residues.serial observations of ddt-contaminated and uncontaminated salt marshes in the norther gulf of mexico were made in november and december, 1973. blue crab (callinectes sapidus) mortalities observed in the ddt-contaminated marsh during this period were correlated with reduced daily temperature minima. gas chromatographic analysis of hepatopancreas and swimmeret muscle tissue of dead and dying crabs revealed total ddt residue concentrations as high as 39.0 ppm and 1.43 ppm, respectively. it is sugg ...19761267483
the structure of the stomatogastric neuromuscular system in callinectes sapidus, homarus americanus and panulirus argus (decapoda crustacea). 19744152406
novel protein inhibits in vitro precipitation of calcium carbonate.organic molecules both coexist and interact with inorganic crystal lattices in biomineralizing tissues. mineral precipitation and crystal morphology are tightly regulated by the actions of these molecules. polyacrylamide gel electrophoresis studies on water soluble extracts from the cuticle of callinectes sapidus (atlantic blue crab) reveal the presence, in unmineralized nascent premolt cuticle, of proteins which are absent in the mineralized postmolt cuticle. in the present studies, homogenates ...19921497356
metal-specific induction of metallothionein isoforms in the blue crab callinectes sapidus in response to single- and mixed-metal exposure.metallothioneins (mts) play an important role in the metabolism of copper and zinc during the molt cycle of the blue crab. in this study we examined the hypothesis that mt expression in crabs is metal specific. anion-exchange chromatography showed one major zncumt (znmt-i) in control crabs, two mt isoforms in cadmium-treated crabs (cdmt-i, cdmt-ii), and three forms (cumt-i, cumt-ii, cumt-iii) in copper-treated animals. amino acid analysis of the carboxymethylated apo-mts, purified by reversed-ph ...19921567201
variations in serum constituents of the blue crab, callinectes sapidus: major cations. 19744154178
principal component analysis, trace elements, and blue crab shell disease. 19921590588
purification and properties of glycogen phosphorylase a from the muscle of the blue crab, callinectes danae. 19744213332
in vivo magnetic resonance imaging of the blue crab, callinectes sapidus: effect of cadmium accumulation in tissues on proton relaxation properties.nuclear magnetic resonance imaging (mri) has been used to visualize the internal anatomy of a living blue crab. the resolution obtained in these studies was sufficient to distinguish individual organs by the differences in their proton densities and proton relaxation properties. t1 (spin-lattice relaxation time)-weighted imaging revealed the lipid-rich nature of the hepatopancreas and gonadal tissue. to evaluate the effect of metal-induced stress on the different organs, crabs were exposed to el ...19921645119
the identification and structure-activity relations of a cardioactive fmrfamide-related peptide from the blue crab callinectes sapidus.the pericardial organs and thoracic ganglia of the blue crab callinectes sapidus were resected and extracted. the extracts were fractionated by hplc and the fractions analyzed by a radioimmunoassay (ria) to fmrfamide. multiple peaks of immunoreactivity were present and one of these, upon fast atom bombardment mass spectrometry (fab-ms) and microsequencing, yielded the sequence gynrsflrfamide. the amount of this peptide in each crab is between 7 and 13 pmol. several incomplete sequences were also ...19911815216
fishing a superfund site: dissonance and risk perception of environmental hazards by fishermen in puerto rico.risk perception studies show that individuals tend to underestimate significant risks, overestimate negligible ones, and distrust authorities. they also rely on a variety of strategies or heuristics to reach decisions regarding their risk-taking behavior. we report on a survey of fishermen and crabbers engaged in recreational and substance fishing in a puerto rican estuary (near humacao), which has been declared a "superfund site" because of suspected contamination by mercury, and at ecologicall ...19911876726
purification and characterization of an iron-binding protein from the blue crab (callinectes sapidus).the presence of an iron-binding protein in the hemolymph of the blue crab (callinectes sapidus) was detected by gel filtration of 59fe-labeled hemolymph. the iron-binding protein was purified to homogeneity by ion exchange chromatography. 2. this protein has a mol. wt of 155,000 and consists of a single polypeptide chain with an isoelectric point of 5.0. 3. analysis of the iron-loaded protein indicates that it has a high affinity for iron and the capacity to bind approximately 10 atoms iron/mole ...19902085965
allostery in callinectes sapidus hemocyanin: cooperative oxygen binding and interactions with l-lactate, calcium, and protons.allosteric interactions of callinectes sapidus hemocyanin have been investigated by generation of precision oxygen-binding curves using a modified imai apparatus and their subsequent analysis using numerical methods. the monod-wyman-changeux (mwc) model, the mwc model extended to include hybrid r3t3 states, and the phenomenological adair equation were fit to the experimental data. the hybrid mwc model provided a quantitatively better fit to the data than did the mwc model. the fits of the hybrid ...19882837279
co binding by hemocyanins of limulus polyphemus, busycon carica, and callinectes sapidus. 19744429663
accumulation of mirex-14c in the adult blue crab (callinectes sapidus). 19744433859
the effects of temperature, salinity and a simulated tidal cycle on the toxicity of fenitrothion to callinectes sapidus.the 96 hr lc50 for callinectes sapidus exposed to fenitrothion at 22 degrees c and a salinity of 34 ppt (parts per thousand) was estimated to be 8.6 micrograms/l with a 95% confidence interval of 7.4-9.9 micrograms/l. acute toxicity was shown to increase with increasing temperature as well as increasing salinity. exposure of callinectes to a simulated tidal cycle of 17 ppt salinity change at 6 hr intervals increased the acute toxicity of fenitrothion to callinectes. the autotomization response i ...19852858335
survival studies with spores of clostridium botulinum type e in pasteurized meat of the blue crab callinectes sapidus.clostridium botulinum type e studies reported in this paper include the incidence of the organism in selected chesapeake bay areas, growth and toxin production in crabmeat homogenates, and the effect of pasteurization upon varying levels of spores in crabmeat. type e spores were detected in 21 of 24 bottom mud samples taken at locations from which blue crabs were being harvested. sterilized crabmeat homogenates inoculated with as little as five spores per 10 g became toxic after 8 days at 50 f, ...19744596746
the effect of dibutyryl camp on sodium uptake by isolated perfused gills of callinectes sapidus.hemolymph, collected from 25 and 100% seawater acclimated callinectes sapidus and perfused through the isolated gill, causes increases in na+ uptake in the gill. similarly, dibutyryl camp causes increases in na+ uptake when perfused through the gill. since pericardial organ (po) extracts dopamine and octopamine are known to increase camp levels in the perfused gill, it is suggested that these substances from the po may be the hemolymph factors which increase na+ uptake via the mediation of camp ...19873028905
evoked activity in the nervous system of callinectes sapidus following phasic excitation of the statocysts. 19724638898
ammonium ion substitutes for k+ in atp-dependent na+ transport by basolateral membrane vesicles.ion-transporting cells from posterior gills of blue crabs (callinectes sapidus) acclimated to low salinity were used as starting material for the preparation of microsomal membrane vesicles by density gradient centrifugation. the na+-k+-adenosinetriphosphatase (atpase)-enriched basolateral vesicles were loaded with kcl- or nh+4-containing medium by dilution and centrifugation, and initial rates of 22na+ uptake into the vesicles were measured by a rapid filtration procedure. varying the extravesi ...19873030142
detection of protein variation in human serum by externally released precipitins from the blue crab, callinectes sapidus.1. a number of invertebrate species releases precipitins into the environment. this process is apparently part of an externally directed immunologic system. 2. a saline solution of precipitins released from the blue crab, callinectes sapidus, provided a reagent that produced two different precipitation reactions with proteins from the sera of human individuals. 3. one of the reaction variants was also detected with mammalian antiserum, the other was not. the former variant was associated with a ...19873427930
correlation between osmoregulation and cell volume regulation.the euryhaline crab, callinectes sapidus, behaves both as an osmoregulator when equilibrated in salines in the range of 800 mosm and below and an osmoconformer when equilibrated in salines above 800 mosm. there exists a close correlation between osmoregulation seen in the whole animal in vivo and cell volume regulation studied in vitro. hyperregulation of the hemolymph osmotic pressure and cell volume regulation both occurred in salines at approximately 800 mosm and below. during long-term equil ...19873565606
chitinoclastic bacteria associated with shell disease in penaeus shrimp and the blue crab (callinectes sapidus). 19734701183
the purification and characterization of glutathione s-transferase from the hepatopancreas of the blue crab, callinectes sapidus.high glutathione s-transferase activity was found in the cytosol of f-cells from the hepatopancreas of the blue crab (callinectes sapidus). purification of glutathione s-transferase from hepatopancreas extracts by sephadex g-200, deae-sephacel, and chromatofocusing resulted in the isolation of two isozymes with isoelectric points of 5.9 and 5.7, as determined by analytical isoelectric focusing. using 1-chloro-2,4-dinitrobenzene as the substrate the specific activities of the two purified isozyme ...19873592675
biochemical and ultrastructural aspects of ca2+ transport by mitochondria of the hepatopancreas of the blue crab callinectes sapidus.mitochondria isolated from the hepatopancreas of the blue crab callinectes sapidus show up to 12-fold stimulation of respiration on addition of ca(2+), which is accompanied by ca(2+) accumulation (ca(2+):site = 1.9) and h(+) ejection (h(+):ca(2+) = 0.85). sr(2+) and mn(2+) are also accumulated; mg(2+) is not. a strongly hypertonic medium (383 mosm), mg(2+), and phosphate are required for maximal ca(2+) uptake. ca(2+) uptake takes precedence over oxidative phosphorylation of adp for respiratory e ...19744827906
nutritional availability to rats of selenium in four seafoods: crab (callinectes sapidus), oyster (crassostrea virginica), shrimp (penaeus duorarum) and baltic herring (clupea harengus).1. the present study was conducted to determine the biological availability to rats of the selenium in four high-se seafoods: crab (callinectes sapidus), oyster (crassostrea virginica), shrimp (penaeus duorarum) and baltic herring (clupea harengus). 2. weanling male rats were fed on a se-deficient torula yeast diet for 4 weeks followed by either continued depletion or repletion for 4 weeks with 0.05, 0.1 or 0.2 microgram se as selenite/g, or 0.1 or 0.2 microgram se as freeze-dried cooked test fo ...19863676154
the salt absorbing cells in the gills of the blue crab (callinectes sapidus rathbun) with notes on modified mitochondria. 19684884645
isolation of vibrio parahaemolyticus from the processed meat of chesapeake bay blue crabs.a method for the recovery of vibrio parahaemolyticus from seafoods is described. by this procedure, a total of 56 biochemically positive cultures of v. parahaemolyticus were recovered from market samples of chesapeake bay processed blue crab (cooked, picked, packed, and refrigerated meat). all of the isolates were tested serologically, and 22 strains were serotyped according to the schema of sakazaki as follows: k3, k5, k28, k31, k36, k37, k39, k43, and k44. these results indicate the broad dist ...19704921056
responses to reversed nh3 and nh4+ gradients in a teleost (ictalurus punctatus), an elasmobranch (raja erinacea), and a crustacean (callinectes sapidus):evidence for nh4+/h+ exchange in the teleost and the elasmobranch.ammonia excretion rates of channel catfish, ictalurus punctatus, little skate (raja erinacea), and blue crab (callinectes sapidus) were measured in experimental regimes which permitted simultaneous assessment of the partial pressure gradients for nonionized nh3 and the chemical concentration gradients of nh4+. under conditions of low external ammonia, the average ammonia excretion was +295 microm kg-1 h-1 for catfish, +149 microm kg-1 h-1 for blue crabs, and +59 microm kg-1 h1 for skates with pa ...19863746231
the uptake and in vivo metabolism of the organophosphate insecticide fenitrothion by the blue crab, callinectes sapidus.callinectes were exposed to [14c]fenitrothion at a level of 5.2 micrograms/liter in either 22 degrees c, 34 ppt; 22 degrees c, 17 ppt; or 17 degrees c, 34 ppt seawater. uptake from the water, as measured by a decrease in fenitrothion, and the distribution of radioactivity throughout the callinectes were determined. the nature of radiolabeled metabolites in the water and hepatopancreas was also determined. fenitrothion was absorbed more rapidly from the water at the higher salinity and temperatur ...19863764904
colonization of the gut of the blue crab (callinectes sapidus) by vibrio cholerae.attachment of vibrio cholerae to the mucosal surface of the intestine is considered to be an important virulence characteristic. vibrio cholerae, an autochthonous member of brackish water and estuarine bacterial communities, also attaches to crustacea, a significant factor in multiplication and survival of v. cholerae in nature. the ability of v. cholerae to attach to the gut wall of the blue crab (callinectes sapidus) was examined, and attachment was observed only in the hindgut and not the mid ...19863767362
ultrastructural study of microsporidian development. i. nosema sp. sprague, 1965 in callinectes sapidus rathbun. 19704986525
heterogeneous humoral and hemocyte-associated lectins with n-acylaminosugar specificities from the blue crab, callinectes sapidus rathbun.callinectes sapidus serum and hemocyte microsomal fraction agglutinated a panel of untreated and enzyme treated vertebrate erythrocytes and cultured lymphoid cell lines. crossed absorption experiments suggested the presence of multiple specific lectins in the serum. the microsomal fraction showed a 35-fold increase in specific activity when compared to the hemocyte lysate suggesting that hemocyte lectins are membrane-associated. agglutination by serum and hemocyte lectins was inhibited by low co ...19863769455
incidence of bacteremia in stressed and unstressed populations of the blue crab, callinectes sapidus.the incidence of bacteremia in the blue crab, callinectes sapidus, is reported to be in excess of 80%. because these results have been controversial, a field study was initiated to determine the effect of commercial capture and handling stresses on the incidence and levels of infection in blue crabs. the majority (75%) of "unstressed" crabs which were captured individually and bled immediately upon removal from the water were bacteremic, with a geometric mean level of infection of 14 cfu/ml of h ...19854051486
biological relationship of callinectes sapidus (rathbun) with carcinonemerteans, bryozoan, and barnacles. 19705412351
electrical properties of axons of callinectes sapidus. 19705459481
bicarbonate-chloride exchange in gill plasma membranes of blue crab.the uptake of chloride was studied in gill plasma membrane vesicles from low-salinity-adapted blue crabs (callinectes sapidus). cl- uptake was not na+ dependent. however, when a hco-3 gradient (in greater than out) was imposed across the membrane, a transient overshoot of about 2.5-fold was produced. approximately 90% of the cl- uptake reflected entry into the osmotically active intravesicular space. cl- itself, nitrate, hydroxyl, and sulfite could substitute for hco-3. the hco-3/cl- exchange pr ...19854061678
potentiometric evidence for the active transport of sodium and chloride across excised gills of callinectes sapidus. 19714150220
conversion of progesterone to 11-deoxycorticosterone by the androgenic gland of the blue crab (callinectes sapidus rathbun). 19695765442
the effect of external salinity upon lipid synthesis in the blue crab callinectes sapidus rathbun and in the spider crab libinia emarginata leech. 19744153714
vibrio parahaemolyticus from the blue crab callinectes sapidus in chesapeake bay.strains of vibrio parahaemolyticus, the etiologic agent of "shirasu" food poisoning in japan, were isolated from moribund blue crabs callinectes sapidus and identified by biochemical and serological techniques.19695770620
the effect of salinity and temperature on the ion levels in the hemolymph of the blue crab, callinectes sapidus, rathbun. 19744153717
the effect of salinity and sex on the respiration rates of excised gills of the blue crab, callinectes sapidus. 19744156238
respiration and phosphorylation by mitochondria from the hepatopancreas of the blue crab (callinectes sapidus). 19734266066
molt cycle regulation of nucleotide pyrophosphatase in the hepatopancreas of the blue crab, callinectes sapidus rathbun. 19694304992
the glucuronic acid pathway in the blue crab, callinectes sapidus rathbun: the enzymes of the udpglucose to glucuronic acid portion of the pathway. 19676035559
effect of single and continuous exposures of gamma radiation on the survival and growth of the blue crab, callinectes sapidus. 19676066585
isosmotic intracellular regulation and redox changes in the respiratory chain components of callinectes sapidus isolated muscle fibers. 19724348290
blood glucose changes in the blue crab callinectes sapidus rathbun on transfer from sea water to air.intertidal decapods regulate their blood glucose with a significant but transitory (60 min) increase upon exposure to atmospheric air, and this has been considered to be an adaptative response related to the almost complete lack of the pasteur effect in facultative anaerobes. in these animals we would not observe an increase in substrate availability to cope with the small amount of energy furnished by anaerobic pathways but rather a general metabolic depression. however, this hypothesis has nev ...19902094547
concurrent protein synthesis is required for in vivo chitin synthesis in postmolt blue crabs.chitin synthesis in crustaceans involves the deposition of a protein-polysaccharide complex at the apical surface of epithelial cells which secrete the cuticle or exoskeleton. the present study involves an examination of in vivo incorporation of radiolabeled amino acids and amino sugars into the cuticle of postmolt blue crabs, callinectes sapidus. rates of incorporation of both 3h leucine and 3h threonine were linear with respect to time of incubation. incorporation of 3h threonine into the endo ...19902250160
bacteriological survey of the blue crab industry.during sanitation inspections of 46 crabmeat processing plants on the atlantic and gulf coasts, 487 samples of whole crabs immediately after cooking, cooked crabs after cooling, backed or washed (or both) crab bodies and whole crab claws, as well as 1,506 retail units of finished product were collected and analyzed bacteriologically. the 1,506 retail units (1-lb [373.24-g] cans) included 518 cans of regular (special) meat, 487 cans of claw meat, and 501 cans of lump meat. statistical analyses sh ...19724568256
incidence of clostridium botulinum in crabmeat from the blue crab.the incidence of clostridium botulinum in fresh crabmeat of blue crab was six out of 986 samples; in pasteurized crabmeat one sample out of 1,000 contained the organism.19744607823
concentration of rotavirus and enteroviruses from blue crabs (callinectes sapidus).a simple method for concentration and detection of rotavirus and enteroviruses in the blue crab is described. virus was separated from tissue homogenates at ph 9.5, concentrated by adsorption to protein precipitates at ph 3.5, and recovered by elution of precipitates at ph 9.2. test samples of 12 to 15 ml were produced from an initial 100 g of crab tissues. cat-floc precipitation was used to remove sample toxicity for cell cultures. recovery effectiveness averaged 52% with poliovirus 1, echoviru ...19836318667
enzymatic mechanisms during temperature acclimation of the blue crab, callinectes sapidus. i. oxygen consumption and activity of glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase. 19724644212
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