| an agglutinin with unique specificity for n-glycolyl sialic acid residues of thyroglobulin in the hemolymph of a marine crab scylla serrata (forskal). | a novel agglutinin with specificity for sialic acid sequence of sugars in thyroglobulin is identified in the hemolymph of scylla serrata. the physico-chemical characteristics of its binding affinity, such as ph and temperature optima, and cationic requirements are defined. n-glycolyl neuraminic acid (neugc) (at 0.6 mm), in contrast to n-acetyl neuraminic acid (neuac) (at greater than 5.0 mm), is the potent inhibitor of hemagglutination. bovine and porcine thyroglobulins containing neugc, inhibit ... | 1992 | 1601116 |
| nmr analysis of the structure and metal sequestering properties of metallothioneins. | multinuclear 1 and 2 dimensional magnetic resonance methods have been used to investigate the structures and metal binding properties of metallothioneins (mts) isolated from several different sources. 113cd nmr studies have unambiguously shown that the 7 g-atoms of cd2+ bound per mole of the mammalian mt are located in two separate metal clusters, one containing 4 metal ions and the other, 3 metal ions. in the invertebrate (scylla serrata) mt, similar studies have revealed that the 6 g-atoms of ... | 1987 | 2959502 |
| nmr studies of crab and plaice metallothioneins. | metallothioneins isolated from the hepatopancreas of the edible crab (cancer paqurus) and the plaice (pleuronectes platessa) after cadmium injection are predominantly cadmium proteins containing only small amounts of zinc and traces of copper. the removal of metal ions from the two metallothioneins by edta was studied using proton nmr spectroscopy. the rates of removal of cadmium and zinc were monitored directly from the intensity of the resonances due to the cadmium and zinc-edta complexes. nea ... | 1986 | 3086076 |
| biological evaluation of crab meat (scylla-serrata) in the rat. | | 1967 | 6022760 |
| crustacean defense strategies ii. recognition, clearance, accumulation and externalization of soluble foreign proteins by the mud-crab scylla serrata. | the defense reactions of scylla serrata have been investigated by challenging crabs with two metalloproteins, horse radish peroxidase (hrp:m.w. 40,000) and hemoglobin (hb:64,000). the defense response analyzed include 1) recognition of foreign proteins; 2) clearance from the haemocoel; 3) accumulation in tissues and 4) externalization. protein clearance was similar to dye clearance in several respects: 1) clearance rate increased with higher a) molecular weight and concentration and b) at night ... | 1984 | 6500133 |
| a spectroscopic study of rat liver and scylla serrata crab metallothioneins. | the absorption, circular dichroism (cd) and magnetic circular dichroism (mcd) spectra of native rat liver and crab (scylla serrata) cd,zn-metallothionein have been measured and the data are compared. the mcd data indicate that there are close similarities in the geometries of the cadmium-binding sites in both of these proteins; however, the cd spectra are quite different for the rat liver and crab proteins. the cd spectrum for the crab metallothionein is unlike any previously reported for a cadm ... | 1984 | 6691985 |
| structure of an invertebrate metallothionein from scylla serrata. | | 1982 | 7061432 |
| structure elucidation of the metal-binding sites in metallothionein by 113cd nmr. | 113cd nmr has been used to determine the structures of the multiple metal-binding sites in the two major isoproteins of metallothionein from mammalian livers (rabbits, calf, and human) and from scylla serrata hepatopancreas. the native protein isolated from the livers of rabbits that had been subjected to repeated injections of 113cdcl2 contains an appreciable amount of zn2+ in addition to 113cd2+, ranging from 2 to 3 g-atoms of a total metal content of 7 g-atoms/mol of protein. the native zn2+ ... | 1982 | 7140998 |
| a sensitive and specific dna probe for the oyster pathogen haplosporidium nelsoni. | haplosporidium nelsoni is a pathogen of the eastern oyster, crassostrea virginica, along the middle atlantic coast of the u.s. genomic dna was extracted from h. nelsoni plasmodia and small subunit (ssu) rdna was amplified by pcr, cloned and sequenced. the sequence of h. nelsoni ssu rdna was aligned with that of another haplosporidian, minchinia teredinis, and with ssu rdna data of c. virginica and various protists in genbank. a 21-base oligonucleotide unique to h. nelsoni, designated msx1347, wa ... | 1995 | 7620459 |
| small subunit ribosomal rna gene sequence of minchinia teredinis (haplosporidia: haplosporidiidae) and a specific dna probe and pcr primers for its detection. | minchinia teredinis is a pathogen of wood-boring molluscs (shipworms), teredo spp., along the middle atlantic coast of the u.s. genomic dna was extracted from m. teredinis spores and small subunit (ssu) rdna was amplified by pcr, cloned, and sequenced. the sequence of m. teredinis ssu rdna was aligned with that of haplosporidium nelsoni and various protists in genbank. a 22-base oligonucleotide probe unique to m. teredinis, designated min702, was commercially synthesized and tested for sensitivi ... | 1995 | 7745284 |
| purification and characterization of a cadmium-induced metallothionein from the shore crab carcinus maenas (l.). | two metallothionein variants were purified from the midgut gland of crabs (carcinus maenas) exposed to a high cadmium concentration (2 p.p.m.). one of the variants was purified from crabs exposed to a low cadmium concentration (0.5 p.p.m.). the purification method involved acetone precipitation, gel filtration and reversed-phase h.p.l.c. the complete amino acid sequences of both variants have been elucidated by m.s. and automated sequence analysis on s-methylated proteins or fragments produced b ... | 1994 | 8110201 |
| purification and characterization of n-glycolyneuraminic-acid-specific lectin from scylla serrata. | a sialic-acid-binding lectin with specificity for n-glycolylneuraminic acid (neugc) was purified from the hemolymph of the marine crab scylla serrata by affinity chromatography using thyroglobulin-coupled agarose. the binding specificity of scylla lectin distinguishes it from other known sialic-acid-specific lectins found in limulus polyphemus and limax flavus, which show a broader range of specificity for sialic acids. the molecular mass of the purified lectin is about 55 kda. under reducing co ... | 1993 | 8354275 |
| effects of experience on crab foraging in a mobile and a sedentary species | the effects of experience on prey and prey-patch choice were compared between two species of marine predatory crabs. the blue crab, callinectes sapidus rathbun, is highly mobile and forages in a variety of estuarine and lagoonal habitats. the atlantic mud crab, panopeus herbstii h. milne-edwards, is smaller and less mobile and is found mostly in oyster reefs and on shelly bottoms. in the laboratory, crabs were offered a choice between two prey types (juvenile hard clams, mercenaria mercenaria li ... | 1997 | 9236012 |
| inhibition of bacterial respiration by a low-molecular weight lectin, scyllin, from scylla serrata crab hemolymph. | interaction of plant and/or invertebrate lectins with mammalian cells and different microorganisms is well known. in the present study, we have demonstrated that scyllin, a low molecular weight (mw 4000) lectin from the edible crab scylla serrata hemolymph, purified by galnac-sepharon affinity column followed by mono-q ion exchanger in fplc exhibits antimicrobial activity against bacillus cereus and escherichia coli by inhibiting endogenous respiration as well as exogenous glucose oxidation. in ... | 1997 | 9343934 |
| influence of naphthalene on protein, carbohydrate, and phosphatases system during the vitellogenesis in marine edible crab, scylla serrata. | | 1998 | 9484552 |
| experimental transmission of white spot syndrome virus (wssv) from crabs to shrimp penaeus monodon. | white spot syndrome virus (wssv) of the black tiger prawn penaeus monodon is a recently discovered baculo-like virus disease which is currently the cause of very serious and widespread losses in the shrimp industry in thailand and elsewhere in asia. three suspected crab carriers of this virus commonly found in shrimp-rearing areas were investigated. these were sesarma sp., scylla serrata and uca pugilator. all these crabs could be infected with wssv by injection and they sustained heavy viral in ... | 1998 | 9789973 |
| isolation and characterization of lectin from thai marine crab (scylla serrata) with binding specificity to sialoglycoconjugates and its application. | a lectin from thai marine carb (scylla serrata) hemolymph has been isolated and purified by affinity column chromatography and preparative electrophoresis. the amino acid composition and 10 amino-terminal residues have been deduced, and its reactivities have been studied using a biotin labeling technique. a method for the determination of sialoglycoconjugates in human serum is described using this lectin. the principle is based on the reaction between the sialoglycoconjugates and biotinylated le ... | 1998 | 9892718 |
| natural and experimental infection of white spot syndrome virus (wssv) in benthic larvae of mud crab scylla serrata. | white spot syndrome virus (wssv), the causative agent of white spot syndrome in shrimp, has a wide host range which extends to crabs, copepods and other arthropods. in this study, benthic larvae of the mud crab scylla serrata were captured from taiwan's coastal waters and screened for the presence of wssv by polymerase chain reaction (pcr) and in situ hybridization. wssv was detected in around 60% of the larvae, and this prevalence rate remained fairly constant when the captured larvae were subs ... | 2000 | 10782350 |
| purification of vitellin from grass shrimp palaemonetes pugio, generation of monoclonal antibodies, and validation for the detection of lipovitellin in crustacea. | much effort has been put into developing vitellogenin antibodies against a wide variety of aquatic vertebrate species to study potential estrogen or anti-estrogen endocrine disrupters. little work has been done on endocrine disruption in aquatic invertebrates. although some antibodies have been produced against blue crab and penaeid shrimp lipovitellin, they have only poor cross-reactivity with the important estuarine grass shrimp, palaemonetes pugio. vitellin was purified from eggs, monoclonal ... | 2000 | 11083030 |
| epizootiology of the parasitic dinoflagellate hematodinium sp. in the american blue crab callinectes sapidus. | hematodinium sp. is a parasitic dinoflagellate that infects and kills blue crabs callinectes sapidus. periodic outbreaks of dinoflagellate infections with subsequent high host mortalities prompted a study of the epizootiology and distribution of the crab pathogen. hemolymph samples from over 13000 crabs were assessed for infections over 8 yr. moderate to high prevalences were found at several locations along the atlantic and gulf coasts of the united states. in the coastal bays of maryland and v ... | 2000 | 11145454 |
| a new shell disease in the mud crab scylla serrata from port curtis, queensland (australia). | in 1994 a previously unreported rust spot shell disease was seen in mud crabs scylla serrata - forskal from port curtis, central queensland, australia. of 673 crabs surveyed, 21.7% had shell lesions. of these, 82.9% had rust spot lesions on the carapace. the majority of rust spot-affected crabs (78.8%) were female. rust spot lesions were predominantly non-perforated (89.4%) and 54.8% were bilaterally symmetrical. there was also a gender difference in the areas of the carapace most commonly affec ... | 2000 | 11206739 |
| effects of periodic hypoxia on mortality, feeding and predation in an estuarine epifaunal community. | the york river estuary, a tributary of the chesapeake bay, usa, experiences periodic low oxygen stress (hypoxia), yet epifaunal species form dense communities there. we studied hypoxia tolerance of common epifaunal species in the york river by exposing sessile and mobile epifauna to high and low oxygen concentrations in laboratory aquaria. mortality in hypoxia varied among species, ranging from 0% to 100%, with trends of decreased tolerance by mobile species relative to sessile species. while mo ... | 2001 | 11278013 |
| risk factors associated with white spot syndrome virus infection in a vietnamese rice-shrimp farming system. | white spot disease (wsd) is a pandemic disease caused by a virus commonly known as white spot syndrome virus (wssv). several risk factors for wsd outbreaks have been suggested. however, there have been very few studies to identify risk factors for wsd outbreaks in culture systems. this paper presents and discusses the risk factors for wssv infection identified during a longitudinal observational study conducted in a vietnamese rice-shrimp farming system. a total of 158 variables were measured co ... | 2001 | 11797910 |
| receptor mediated yolk protein uptake in the crab scylla serrata: crustacean vitellogenin receptor recognizes related mammalian serum lipoproteins. | the receptor-mediated uptake of major yolk protein precursor, vitellogenin (vg) is crucial for oocyte growth in egg laying animals. in the present study plasma membrane receptor for vg was isolated from the oocyte of the red mud crab, scylla serrata. vitellogenin receptor (vgr) protein was visualized by ligand blotting using labeled crab vg ((125)i-vg) as well as labeled low density lipoprotein ((125)i -ldl) and very low density lipoprotein ((125)i-vldl) isolated from rat. the endocytosis of vg ... | 2002 | 11891926 |
| profilicollis novaezelandensis n. sp. (polymorphidae) and two other acanthocephalan parasites from shore birds (haematopodidae and scolopacidae) in new zealand, with records of two species in intertidal crabs (decapoda: grapsidae and ocypodidae). | profilicollis novaezelandensis n. sp. (acanthocephala: polymorphidae) is described from the south island pied oystercatcher haematopus ostralegus finschi martens (haematopodidae) and the intertidal crab hemigrapsus crenulatus (milne edwards) (brachyura: grapsidae) from the south island of new zealand. the new species can be distinguished from all the other species of the genus by a combination of the following characters: long neck (13% of total body length for adults) and a subspherical probosc ... | 2002 | 12023562 |
| instability of crab vitellogenin and its immunological relatedness with mammalian atherogenic lipoproteins. | vitellogenesis is the process of accumulation of vitellogenin (vg) in rapidly growing oocytes of oviparous animals and its' subsequent transformation into lipovitellin (lv). lipovitellin, which forms the major yolk protein, serves as a principal nutrient reserve for the developing embryo. in the present study, vg and lv were purified from the hemolymph and ovary, respectively of the crab scylla serrata by gel filtration followed by preparative gel electrophoresis. it was observed that purified v ... | 2003 | 12548665 |
| evidence of paralytic shellfish poisoning toxin in milkfish in south taiwan. | natural phytoplankton blooms of the dinoflagellate alexandrium minutum, milkfish (chanos chanos) exposed to natural blooms, sediment and mangrove crab (scylla serrata) were analysed for paralytic shellfish poisoning toxins by high-performance liquid chromatography. the toxin profiles of milkfish and mangrove crab were similar to that of a. minutum collected from blooming fishponds. in a laboratory a. minutum-blooming environment, the stomach and intestine of milkfish accumulated paralytic shellf ... | 2003 | 12881129 |
| larval spirurida (nematoda) from the crab macrophthalmus hirtipes in new zealand. | previously undescribed third-stage larvae of two species of spirurida were found in the haemocoel of the stalk-eyed mud crab macrophthalmus hirtipes (heller) (ocypodidae) in new zealand. examinations by light and scanning electron microscopy showed that the larger larvae (about 7 mm long) belonged to a species of ascarophis van beneden, 1871 (cystidicolidae), the genus including parasites of fishes, whereas the smaller larvae (about 4-5 mm long) belonged to the acuariidae, a family with species ... | 2003 | 14560971 |
| pcr-based detection of white spot syndrome virus in cultured and captured crustaceans in india. | the occurrence and distribution of white spot syndrome virus (wssv) among cultured and captured penaeid shrimps and crustaceans in the east coast of india was determined from november 1999 to april 2002 using pcr as a diagnostic tool. | 2003 | 14633096 |
| experimental infection of twenty species of indian marine crabs with white spot syndrome virus (wssv). | twenty species of indian marine crabs were experimentally infected with white spot syndrome virus (wssv), via the oral route and intramuscular injection, to determine their viral susceptibility. we determined that 16 species (calappa philargius, charybdis annulata, c. lucifera, doclea hybrida, grapsus albolineatus, halimede ochtodes, liagore rubronaculata, lithodes maja, matuta miersi, paradorippe granulata, parthenope prensor, philyra syndactyla, podophthalmus vigil, portunus sanquinolentus, sc ... | 2003 | 14735935 |
| the peroxinectin of white shrimp litopenaeus vannamei is synthesised in the semi-granular and granular cells, and its transcription is up-regulated with vibrio alginolyticus infection. | peroxinectin mrna expression in the different types of haemocytes of white shrimp litopenaeus vannamei was studied by in situ hybridisation using digoxigenin-utp-labelled riboprobes and reverse transcription-polymerase chain reaction (rt-pcr). granular cells (gc) and a mixture of semi-granular cells (sgc) and hyaline cells (hc) were separated by 70% percoll gradient centrifugation. peroxinectin was synthesised in both gc and the mixture of sgc-hc. an in situ hybridisation assay indicated that pe ... | 2005 | 15683919 |
| cloning and characterisation of a serine proteinase from the haemocytes of mud crab scylla serrata. | a serine proteinase (sp) cdna was cloned from the haemocytes of mud crab scylla serrata using oligonucleotide primers and rt-pcr. both 3'- and 5'-regions were isolated by rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the cdna clone has an open reading frame of 1,131 bp encoding a protein of 376 amino acids. the calculated molecular mass of the sp mature protein is 39.54 kda with an estimated pi of 5.37. the c-terminal half of s. serrata sp is co ... | 2006 | 16326112 |
| cloning and characterisation of a prophenoloxidase from the haemocytes of mud crab scylla serrata. | a prophenoloxidase (propo) cdna was cloned from the haemocytes of mud crab scylla serrata using oligonucleotide primers and rt-pcr. both 3'- and 5'-regions were isolated by rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the cdna clone has a full length of 2663bp, with an open reading frame of 2019bp, a 124-bp 5'-untranslated region, and a 520-bp 3'-untranslated region containing a poly a signal. it encodes a protein of 673 amino acids with a pred ... | 2007 | 16806468 |
| a new parasite that infects eggs of the mud crab, scylla serrata, in australia. | the mud crab, scylla serrata, is currently being evaluated for its feasibility for mass aquaculture in australia. as part of the evaluation process, pathogens that can affect this species need to be identified. this research note describes a possible new parasite that infects the eggs of s. serrata. the parasite was discovered in two separate cases (three months apart) in a broodstock research program and appears to cause 100% egg mortality. the parasite consists of a cluster of cells with rhizo ... | 2006 | 16884735 |
| molecular cloning and characterisation of a proteinase inhibitor, alpha 2-macroglobulin (alpha2-m) from the haemocytes of tiger shrimp penaeus monodon. | an alpha 2-macroglobulin (alpha2-m) gene was cloned from the haemocytes of tiger shrimp penaeus monodon by rt-pcr, cloning and sequencing of overlapping pcr and rapid amplification of cdna ends (race) method. analysis of the nucleotide sequence revealed that the alpha2-m cdna consists of 4876 bp with an open reading frame (orf) of 4494 bp, a 52 bp 5'-untranslated region, and a 327 bp 3'-untranslated region containing a poly a signal. the open reading frame encodes a protein of 1498 amino acids w ... | 2007 | 16982096 |
| isolation and expression analysis of multiple isoforms of putative farnesoic acid o-methyltransferase in several crustacean species. | farnesoic acid o-methyltransferase (famet) is the enzyme responsible for the conversion of farnesoic acid (fa) to methyl farnesoate (mf) in the final step of mf synthesis. multiple isoforms of putative famet were isolated from six crustacean species belonging to the families portunidae, penaeidae, scyllaridae and parastacidae. the portunid crabs portunus pelagicus and scylla serrata code for three forms: short, intermediate and long. two isoforms (short and long) were isolated from the penaeid p ... | 2007 | 16999957 |
| a male-specific expression gene, encodes a novel anionic antimicrobial peptide, scygonadin, in scylla serrata. | scygonadin is a novel antimicrobial peptide, which was originally isolated from the seminal plasma of the mud crab, scylla serrata. based on the partial 20-residue nh(2)-terminal sequence of the peptide, h-gly-gln-ala-leu-asn-lys-leu-met-pro-lys-ile-val-ser-ala-ile-ile-tyr-met-val-gly-oh, scygonadin was cloned from the gonads of s. serrata using a degenerated reverse transcriptase (rt)-pcr and rapid amplification of cdna ends (race). the full-length cdna sequence contains an open reading frame o ... | 2007 | 17092560 |
| occurrence and density of vibrio parahaemolyticus in live edible crustaceans from markets in china. | vibrio parahaemolyticus is a marine bacterium causing foodborne disease. occurrence of the bacterium was investigated in six species of edible crustaceans available from markets in mainland china. the bacterium was detected in 22 of 45 whole-body, shell, and feces samples, including mitten crabs, which are supposed to be produced in freshwater ponds. the mean densities ranged from 2.8 log cfu/g in mitten crabs (eriocheir sinensis) to 5.1 cfu/g in giant tiger prawns (penaeus monodon). in hemolymp ... | 2006 | 17133820 |
| a reovirus disease in cultured mud crab, scylla serrata, in southern china. | a reovirus, designated mud crab reovirus (mcrv), associated with large economic losses was recently isolated from marine cultured mud crab, scylla serrata, in southern china. the complete viral particle is 70 nm in diameter, icosahedral and non-enveloped. the virus infects connective tissue cells of the hepatopancreas, gills and intestine in mud crab and develops in the cytoplasm. hundred per cent mortality was observed in mud crab experimentally infected by intramuscular injection, bath inocula ... | 2007 | 17352788 |
| antilipopolysaccharide factor (alf) of mud crab scylla paramamosain: molecular cloning, genomic organization and the antimicrobial activity of its synthetic lps binding domain. | antilipopolysaccharide factors (alfs) are small basic proteins that can bind and neutralize lipopolysaccharide (lps) and have broad spectrum antimicrobial activities. in this study, we describe the isolation of the full-length cdna encoding for alf peptide (alfsp) of mud crab, scylla paramamosain by sequencing a hemocyte cdna library and using the rapid amplification cdna end (race) method. a full-length alfsp cdna of 614 bp contains an open reading frame (orf) of 372 bp, encoding 123 amino acid ... | 2007 | 17368541 |
| sustainability and local people's participation in coastal aquaculture: regional differences and historical experiences in sri lanka and the philippines. | this article discusses environmental sustainability in aquaculture and its contribution to poverty alleviation, based on field studies in sri lanka and the philippines. the aquaculture practices studied are the monoculture of the black tiger prawn (penneaus monodon) and milkfish (chanos chanos) and the polyculture of the two species together with the mud crab (scylla serrata). factors affecting economic viability, social equity and environmental impacts in aquaculture are discussed and used to i ... | 2007 | 17929084 |
| identification of the extracellular copper-zinc superoxide dismutase (eccuznsod) gene of the mud crab scylla serrata and its expression following beta-glucan and peptidoglycan injections. | an extracellular copper-zinc superoxide dismutase (eccuznsod, or ec-sod) gene was cloned from haemocytes of mud crab scylla serrata. the eccuznsod cdna consists of 939 bp with an open reading frame (orf) of 585 bp that encodes 195 amino acids (aa) with 31 residues of a signal peptide sequence. the predicted molecular mass of the mature protein (164aa) is 17.1 kda with an estimated pi of 6.89. amino acids responsible for binding cu (his84, 86, 101, and 163) and zn (his101, 109, and 121, and asp 1 ... | 2008 | 17949815 |
| simulated predator extinctions: predator identity affects survival and recruitment of oysters. | the rate of species loss is increasing at a global scale, and human-induced extinctions are biased toward predator species. we examined the effects of predator extinctions on a foundation species, the eastern oyster (crassostrea virginica). we performed a factorial experiment manipulating the presence and abundance of three of the most common predatory crabs, the blue crab (callinectes sapidus), stone crab (menippe mercenaria), and mud crab (panopeus herbstii) in estuaries in the eastern united ... | 2008 | 18409432 |
| development of an rt-pcr detection method for mud crab reovirus. | mud crab reovirus (mcrv) causes high mortality in the cultivated mud crab. to control better an outbreak of this virus, a rapid, specific and sensitive detection method based on rt-pcr was developed. the mcrv detection method was designed based on the one-step and two-step rt-pcr which resulted in the amplification of predicted products of 433 and 304 bp. the method is specific as no cross-reaction was observed between grass carp hemorrhage virus (gchv), white spot syndrome virus (wssv), tiger f ... | 2008 | 18572256 |
| experimental infections reveal that common thai crustaceans are potential carriers for spread of exotic taura syndrome virus. | taura syndrome virus (tsv) was first reported as a serious cause of shrimp mortality limited to reared penaeus (litopenaeus) vannamei in the americas, where it spread principally through regional and international transfer of live post larvae (pl) and broodstock. subsequently, through importation of infected broodstock, tsv outbreaks spread to asia, first to taiwan and china and then to thailand, indonesia and korea. since its introduction to thailand, outbreaks have occasionally been reported f ... | 2008 | 18589994 |
| elemental composition in mud crab scylla serrata from mahanadi estuary, india: in situ irradiation analysis by external pixe. | during the present study concentration of nine elements (k, ca, mn, fe, cu, zn, se, br and pb) in different tissues of mud crab scylla serrata from mahanadi estuary, india were determined by the external pixe set up at institute of physics, bhubaneswar, india. the study demonstrates the effectiveness of the technique in analyzing both soft and hard tissue samples from marine organisms and opens the door for non-destructive, multi-elemental analysis of tissue samples with a very little sample pre ... | 2009 | 18996431 |
| development of primary cell culture from scylla serrata : primary cell cultures from scylla serrata. | this paper reports for the first time, the primary cell culture of hepatopancreas from edible crab scylla serrata using crab saline, l-15 (leibovitz), 1 x l-15 + crab saline, 2 x l-15 + crab saline, 3 x l-15 and citrate buffer without any serum. we could isolate and maintain e (embryonalzellen), f (fibrenzellen), b (blasenzellen), r (restzellen) and g (granular cells). upon seeding the hepatopancreatic e, f, b, and r cells showed different survival pattern over time than granular cells. a modifi ... | 2008 | 19002854 |
| habitat complexity influences cascading effects of multiple predators. | although multiple predator effects and trophic cascades have both been demonstrated in a wide variety of ecosystems, ecologists have yet to incorporate these studies into an experimental framework that also manipulates a common and likely important factor, spatial heterogeneity. we manipulated habitat complexity, the presence of two top predators (toadfish and blue crabs), and one intermediate predator (mud crabs) to determine whether habitat complexity influences the strength of multiple predat ... | 2008 | 19137947 |
| identification and cloning of the alpha2-macroglobulin of giant freshwater prawn macrobrachium rosenbergii and its expression in relation with the molt stage and bacteria injection. | the full-length complementary (c)dna of the alpha2-macroglobulin (alpha2m) gene was cloned from haemocytes of the giant freshwater prawn macrobrachium rosenbergii by reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) methods. the 4875-bp cdna contains an open reading frame (orf) of 4419 bp, a 95-bp 5'-untranslated region (utr), and a 361-bp 3' utr containing the poly a tail. the orf encodes a protein of 1472 amino acids (aa) with a 23-residue sign ... | 2009 | 19340942 |
| identification, cloning, characterization and recombinant expression of an anti-lipopolysaccharide factor from the hemocytes of indian mud crab, scylla serrata. | anti-lipopolysaccharide factors (alf) are a group of small basic proteins which are released into the hemolymph as a result of rapid degranulation of hemocytes in response to bacterial lipopolysaccharide (lps). in the present study, using a combined approach of degenerate and race pcr, the gene coding for scylla serrata anti-lipopolysaccharide factor (ssalf) was cloned and characterized. the full-length ssalf cdna sequence consists of 607 bp and encodes a polypeptide of 97 amino acids, constitut ... | 2009 | 19490944 |
| trace element-based food value evaluation in soft and hard shelled mud crabs. | concentrations of 10 elements were studied from the pre-moult hard shelled and newly moulted (soft shelled) crabs (scylla serrata) by the (energy dispersive x-ray fluorescence) edxrf technique. the study evaluated the composition of all 10 elements from the body tissues and the exuvium of soft shelled and the carapace of pre-moulted hard-shelled crabs in order to evaluate the food value from nutritional and safety point of view for human consumption. the essential elements like k, ca, mn, cu, et ... | 2009 | 19660512 |
| molecular cloning of mud crab scylla serrata peroxinectin and its expression following vibrio alginolyticus and peptidoglycan injections. | | 2010 | 19751834 |
| detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification. | red-spotted grouper nervous necrosis virus (rgnnv) causes high mortality in marine fish larvae cultured in china. to control better an outbreak of this virus, a rapid, specific and sensitive detection method based on loop-mediated isothermal amplification (lamp) was developed. a set of four primers, two outer and two inner, was designed from rgnnv genome rna. the lamp reaction mix was optimized. the method was specific as no cross-reaction was observed between white spot syndrome virus, koi herp ... | 2010 | 19755132 |
| soluble expression and purification of a crab antimicrobial peptide scygonadin in different expression plasmids and analysis of its antimicrobial activity. | scygonadin is an anionic antimicrobial peptide recently identified from the seminal plasma of scylla serrata. to gain more detailed information on its antimicrobial activity, scygonadin mature peptide was expressed in escherichia coli in order to obtain a large quantity of biologically active product. an approximately 43 kda fusion protein cks-scygonadin was obtained in a highly stable and soluble form. the soluble component of the fusion cks-scygonadin was purified by immobilized metal affinity ... | 2010 | 19766724 |
| investigation of the mud crab (scylla serrata) as a potential bio-monitoring species for tropical coastal marine environments of australia. | mud crabs, scylla serrata, were sampled from four estuaries (the normanby, herbert, burdekin and fitzroy rivers) along the coast of northern queensland, australia, representing a pollution gradient from low to high contamination based upon previous chemical monitoring. four biomarkers; glutathione-s-transferase (gst) activity, cholinesterase (che) inhibition and the urinary metabolite concentrations of naphthalene (nph) and benzo-a-pyrene (bap) were evaluated in s. serrata hepatopancreas, haemol ... | 2010 | 19819475 |
| hepatopancreas cell cultures from mud crab, scylla paramamosain. | hepatopancreas is an important digestive and endocrine organ in crustacean. however, there are few reports on cell cultures from crabs. here, the cell cultures of hepatopancreas from scylla paramamosain was studied in vitro. both the primary cell culture and subculture were grown in leibovitz' l-15 medium, m199 medium, or a specially designed medium for s. paramamosain (msp). the results showed that hepatopancreas cells in vitro grew in compact clusters in 2-3 d. four types of cells could be ide ... | 2010 | 19915927 |
| molecular cloning and expression of a 2-cys peroxiredoxin gene in the crustacean eurypanopeus depressus induced by acute hypo-osmotic stress. | peroxiredoxins (prxs) are a family of ubiquitous proteins that help minimize the harmful effects of oxidative stress by catalyzing the reduction of hydrogen peroxide (h2o2) and organic hydroperoxides to less harmful forms. a full-length cdna corresponding to a 2-cys prx gene was isolated from the flatback mud crab eurypanopeus depressus and designated as edprx-1 (genbank accession no. eu684547). edprx-1 has a major open-reading frame of 594 bp and is capable of encoding a polypeptide of 198 amin ... | 2010 | 19961951 |
| structural and functional comparisons and production of recombinant crustacean hyperglycemic hormone (chh) and chh-like peptides from the mud crab scylla olivacea. | sco-chh and sco-chh-l (chh-like peptide), two structural variants of the crustacean hyperglycemic hormone family identified in the mud crab (scylla olivacea), are presumably alternatively spliced gene products. in this study, sco-chh and sco-chh-l were isolated from the tissues using high performance liquid chromatography. identity of the native peptides was confirmed using mass spectrometric (ms) analyses of purified materials and of trypsin-digested peptide fragments. additionally, characteriz ... | 2010 | 20171218 |
| molecular cloning and characterization of alpha 2-macroglobulin (alpha2-m) from the haemocytes of chinese mitten crab eriocheir sinensis. | alpha 2-macroglobulin (alpha2-m) is a large protein in blood and is able to inactivate a variety of proteinases in invertebrates and vertebrates. the alpha2-m gene was obtained from the chinese mitten crab, eriocheir sinensis, by the reverse transcript-polymerase chain reaction (rt-pcr) and the rapid amplification of cdna ends (race). the alpha2-m cdna of e. sinensis contained 4851 bp with an open reading frame of 4371 bp, a 70 bp 5'-untranslated region, a 410 bp 3'-untranslated region, and thre ... | 2010 | 20394825 |
| molecular detection of hematodinium sp. in northeast pacific chionoecetes spp. and evidence of two species in the northern hemisphere. | hematodinium is a genus of parasitic dinoflagellates that infects crustaceans worldwide including tanner crabs chionoecetes bairdi and snow crabs c. opilio in the northeast pacific ocean. the present study describes the optimization of a pcr-based assay for the detection and monitoring of hematodinium sp. in snow and tanner crabs. two fragments, 1682 and 187 bp, were amplified from the 18s ribosomal dna region of the parasite. the assay performed well in 6 additional decapod species (1 lobster a ... | 2010 | 20402233 |
| bacteria associated with wild mud crab (scylla serrata) from setiu wetland, malaysia with emphasis on antibiotic resistances. | a study was carried out to investigate the presence of bacteria flora in wild mud crab (scylla serrata) from setiu wetland as well as their antibiotic resistances. a total of 91 bacterial isolates consisting of 12 bacterial species were successfully isolated from mud crab. oxolinic acid was found to be effective against all the bacterial isolates whilst the highest percentage of antibiotic resistance was shown by lincomycin (94.5%) followed by ampicillin (90.1%), amoxicillin (86.8%) and oleandom ... | 2010 | 20506717 |
| concentrations of some heavy metals in water, suspended solids, and biota species from maluan bay, china and their environmental significance. | concentrations of heavy metals (cu, zn, cd, and pb) in surface water (including total recoverable, dissolved, suspended solids) and in aufwuchs encrusted on moerella iridescens benson from seven selected sites and two reference sites in maluan bay were investigated in order to understand current metal contamination due to industrialization and urbanization in xiamen, china. the muscle tissues of the study species (penceus penicillatus, scylla serrata forskal, harengula zunasi bleeker, tillapia n ... | 2011 | 20508984 |
| primary culture and characteristic morphologies of neurons from the cerebral ganglion of the mud crab, scylla paramamosain. | crustacean neurons, obtained from the cerebral ganglion of the mud crab scylla paramamosain, were successfully cultured in vitro. they maintained typical morphological characteristics and showed better outgrowth in modified medium 199 (m199) medium than that in liebowitz's l-15 medium. fetal bovine serum (fbs), muscle extracts, and hemolymph of the mud crab s. paramamosain were added as supplements. only 20% fbs could promote neuron outgrowth, while muscle extracts and hemolymph of s. paramamosa ... | 2010 | 20585894 |
| molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab portunus trituberculatus. | an extracellular cuznsod cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the eccuznsod full-length cdna consisted of 965 bp with an open reading frame of 579 bp. it encoded a polypeptide of 192 amino acids which had a predicted molecular weight of 20.0 kda and with an estimated pi of 6.23. the deduced amin ... | 2011 | 20848212 |
| molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (alf) from the mud crab, scylla paramamosain. | antimicrobial peptides (amps) serve a major role in host defense systems against microbial invasion. in this study, a novel isoform (alfsp2) of antilipopolysaccharide factors (alfs) was cloned from the mud crab, scylla paramamosain. the open reading frame of the alfsp2 cdna is 348 bp and encodes for a predicted 115 amino acid residues (12.92 kda), and a mature protein of 94 amino acids and a molecular mass of 10.79 kda. the amino acid sequence of alfsp2 has an overall similarity of 74%, 66% and ... | 2010 | 20883796 |
| monoclonal antibodies produced against vp3 of a novel mud crab dicistrovirus. | mud crab dicistrovirus (mcdv) is a recently identified single-stranded positive rna virus, which causes serious economic loss. the three structural proteins of mcdv were separated by sds-page. in this study, a recombinant mcdv-vp3 was successfully expressed by an escherichia coli expression system. after immunization and cell fusion, three mouse hybridomas (1g6h9, 1e7b8, 4b6e10) producing mabs against mcdv-vp3 were established. the mabs obtained were fully characterized using indirect elisa and ... | 2010 | 21050046 |
| identification and agglutination properties of hemocyanin from the mud crab (scylla serrata). | infectious diseases have significantly delayed the growth of crab aquaculture. identification of the immune molecules and characterization of the defense mechanisms will be pivotal to the reduction of these diseases. hemocyanin is an important non-specific immune protein present in the hemolymph of both mollusks and arthropods. however, little is known about the hemocyanin from the mud crab scylla serrata. in this study, we identified the s. serrata hemocyanin using affinity proteomics and inves ... | 2010 | 21078399 |
| mud crab susceptibility to disease from white spot syndrome virus is species-dependent. | abstract: | 2010 | 21092125 |
| nucleotide sequences of four rna segments of a reovirus isolated from the mud crab scylla serrata provide evidence that this virus belongs to a new genus in the family reoviridae. | this is the first sequence-based characterization of mud crab (scylla serrata) reovirus (ssrv), which causes severe disease of cultured mud crabs in southern china. we sequenced and analyzed genome segments s1, s2, s3, and s7, which were 4,327, 2,721, 2,715, and 1,517 nucleotides long, respectively. conserved motifs were found at the 5' (auaaau) and 3' (aacgau) ends of each segment. rna segments s1, s2, s3, and s7 each contained a single open reading frame (orf) that encoded predicted proteins o ... | 2010 | 21153426 |
| antibacterial activity of a synthetic peptide that mimics the lps binding domain of indian mud crab, scylla serrata anti-lipopolysaccharide factor (ssalf) also involved in the modulation of vaginal immune functions through nf-kb signaling. | recently the cdna coding for anti-lipopolysaccharide factor (alf) has been identified from the indian mud crab, scylla serrata and has been named s. serrata anti-lipopolysaccharide factor (ssalf). ssalf protein sequence demonstrated the presence of two highly conserved cystine residues between which the putative lipopolysaccharide (lps) binding domain is known to be located. in this study, we have designed and synthesized a 24 amino acid linear (lssalf24) and a cyclic (cssalf24) peptides based o ... | 2010 | 21195157 |
| two novel antimicrobial peptides, arasin-likesp and grpsp, from the mud crab scylla paramamosain, exhibit the activity against some crustacean pathogenic bacteria. | antimicrobial peptides (amps) are some of the important host molecules required to resist pathogen infection. two novel amps (arasin-likesp and grpsp) were identified from the hemocytes of the mud crab, scylla paramamosain, by analysis of a hemocyte expressed sequence tag library. the deduced open reading frames of the arasin-likesp and grpsp cdnas are 198 and 168 bp, and encode for predicted peptides of 65 and 55 amino acid residues, respectively. the calculated molecular mass of the mature pep ... | 2011 | 21220028 |
| studies on pathogenicity and prevalence of white spot syndrome virus in mud crab, scylla serrata (forskal), in zhejiang province, china. | mud crab, scylla serrata (forskal), is the most commercially important marine crab species in china. in recent years, serious diseases have occurred in major mud crab culture regions in se china. pcr detection of white spot syndrome virus (wssv) in diseased mud crabs collected from zhejiang province during 2006-2008 showed a prevalence of 34.82%. to study the pathogenicity of wssv to mud crab, healthy mud crabs were injected intramuscularly with serial 10-fold dilutions of a wssv inoculum. the c ... | 2011 | 21241320 |
| purification, cloning, expression and immunological analysis of scylla serrata arginine kinase, the crab allergen. | although crustaceans have been reported to be one of the most common causes of ige-mediated allergic reactions, there are no reports about the characterization and identification of arginine kinase (ak) from the mud crab (scylla serrata) as allergen. in the present study, the purification, molecular cloning, expression and immunological analyses of the ige allergen ak from the mud crab were investigated. | 2011 | 21432856 |
| involvement of peroxinectin in the defence of red swamp crayfish procambarus clarkii against pathogenic aeromonas hydrophila. | cell adhesion factors are important immune components for invertebrate to immobilize, phagocytose or encapsulate invasive microorganisms and foreign particles. in this study, a new cell adhesion factor, peroxinectin (refered as pcpxin) was isolated from hemocytes of red swamp crayfish (procambarus clarkii). the full-length cdna of pcpxin was 3014 bp encoding a protein of 819 amino acid residues with a predicted molecular weight of 89.0 kda and a calculational isoelectric point of 6.93. the putat ... | 2011 | 21539922 |
| in silico prediction and characterization of 3d structure and binding properties of catalase from the commercially important crab, scylla serrata. | the enzyme catalase breaks down h(2)o(2), a potentially harmful oxidant, to h(2)o and o(2). besides oxidase activity, the enzyme also exhibits peroxidase activity. therefore, it plays an important role in maintaining health and regulating pathophysiology of the organisms. however, 3d structure of this important enzyme in invertebrates particularly in crabs is not yet available. therefore, an attempt has been made to predict the structure of the crab catalase and to envisage its catalytic interac ... | 2011 | 21541840 |
| trace elements in edible shellfish species from the lower gangetic delta. | the accumulation of trace elements in edible shellfish species may lead to serious health problems through the food chain. in this study we measured the concentrations of zn, cu, pb and cd in the shellfish species like penaeus monodon, penaeus indicus, scylla serrata, saccostrea cucullata and crassostrea madrasensis using a icp mass spectrometer. samples were collected from the lower stretch of the river ganga which receives wastes of complex character from industries and various anthropogenic a ... | 2011 | 21570722 |
| isolation and identification of acholeplasma sp. from the mud crab, scylla serrata. | for the first time, a mollicute-like organism (mlo) was cultured from moribund mud crabs (scylla serrata) during an outbreak of clearwater disease in zhejiang province, china. the mlo displayed a fried-egg colony morphology in culture, did not possess a cell wall, and was not retained by 0.45?µm and 0.2?µm filters. it was able to ferment glucose, sucrose, lactose, and maltose, but it did not utilize arginine and urea. the mlo grew in the absence of bovine serum and was not susceptible to digiton ... | 2011 | 21808652 |
| induction of mud crab (scylla paramamosain) tropomyosin and arginine kinase specific hypersensitivity in balb/c mice. | background: shellfish hypersensitivity is among the most common food allergies. the allergens tropomyosin (tm) and arginine kinase (ak) from mud crab (scylla paramamosain) were purified to homogeneity. balb/c female mice were sensitized with tm and ak by intragastric administration. mice treated with normal saline served as the negative control (nc) group. results: compared with nc group, mice that were treated with tm and ak developed reduced activity; meanwhile, their scratching behavior and s ... | 2011 | 21780134 |
| Molecular cloning and expression profiles of nitric oxide synthase (NOS) in mud crab Scylla paramamosain. | The importance of the nitric oxide synthase (NOS) gene family is demonstrated by many studies in recent years. However, it keeps unknown of nitric oxide (NO) system and NOS gene family in mud crab Scylla paramamosain, an important cultured commercial crustacean in China and Pacific area. In this report, the cDNA of NOS contained full-length ORF was cloned from mud crab, S.paramamosain. It was of 4424 bp, including a 5'-terminal untranslated region (UTR) of 239 bp, a 3'-terminal UTR of 540 bp, wh ... | 2011 | 22200639 |
| Morphological and Molecular Characterization of Hematodinium perezi (Dinophyceae: Syndiniales), a Dinoflagellate Parasite of the Harbour Crab, Liocarcinus depurator. | Hematodinium perezi Chatton and Poisson (Dinophyceae: Syndiniales) is reported from one of its type hosts, Liocarcinus depurator, from Rye Bay in the English Channel, a site in a similar geographical location to that of the type description. The histology and ultrastructure of vegetative trophont stages, and rDNA sequences of the parasite infecting this host are reported for the first time. Ultrastructurally, H. perezi was confirmed by the presence of condensed chromatin profiles, trichocysts, a ... | 2011 | 22092696 |
| cloning and characterisation of the sptoll gene from green mud crab, scylla paramamosain. | toll/toll-like receptors (tlrs), one of the most important pattern recognition receptors (prrs), play a crucial role in innate immune responses in both invertebrates and vertebrates. in this study, we cloned and characterised a toll gene from scylla paramamosain (sptoll). bioinformatic analysis predicted that sptoll contained one open reading frame of 3018bp and encoded a single-pass transmembrane domain protein of 1005 amino acids. further, sptoll could be clustered into one branch along with o ... | 2011 | 21945833 |
| permanent genetic resources added to molecular ecology resources database 1 june 2011-31 july 2011. | this article documents the addition of 112 microsatellite marker loci and 24 pairs of single nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: agelaius phoeniceus, austrolittorina cincta, circus cyaneus, circus macrourus, circus pygargus, cryptocoryne × purpurea ridl. nothovar. purpurea, mya arenaria, patagioenas squamosa, prochilodus mariae, scylla serrata and scytalopus speluncae. these loci were cross-t ... | 2011 | 21951598 |
| histopathological characterization and in situ detection of callinectes sapidus reovirus. | a reovirus (tentatively designated as callinectes sapidus reovirus, csrv) was found in the blue crabs c. sapidus collected in chesapeake bay in 2005. histological examination of hepatopancreas and gill from infected crabs revealed eosinophilic to basophilic, cytoplasmic, inclusions in hemocytes and in cells of connective tissue. a cdna library was constructed from total rna extracted from hemolymph of infected crabs. one clone (designated as csrv-28) with a 532-bp insert was 75% identical in nuc ... | 2011 | 21925184 |
| sequence analysis of 12 genome segments of mud crab reovirus (mcrv). | mud crab reovirus (mcrv) is the causative agent of a serious disease with high mortality in cultured mud crab (scylla serrata). this study sequenced and analyzed 12 genome segments of mcrv. the 12 genome segments had a total length of 24.464kb, showing a total g+c content of 41.29% and predicted 15 orfs. sequence analysis showed that the majority of mcrv genes shared low homology with the counterpart genes of other reoviruses, e.g., the amino acid identity of rna-dependent rna polymerase (rdrp) ... | 2012 | 22088215 |
| natural host-range and experimental transmission of laem-singh virus (lsnv). | slow growth caused by viral diseases has become a major constraint in shrimp aquaculture. laem-singh virus (lsnv), a positive-sense single-stranded rna (ssrna) virus, has been identified in penaeus monodon showing slow growth syndrome. to examine the host-range and transmission modes of the virus, 6 species of penaeid shrimp of varying life stages, sourced from the wild and from farms, as well as juvenile mud crabs scylla serrata, were screened using rt-nested pcr. lsnv was detected in p. monodo ... | 2011 | 21991662 |
| rapid and sensitive detection of mud crab scylla serrata reovirus by a reverse transcription loop-mediated isothermal amplification assay. | scylla serrata reovirus (ssrv) is one of the most prevalent viral pathogens of the mud crab (s. serrata). this pathogen is widespread in east china and causes severe economic losses to the nation's mud crab industry. early detection of this pathogen is necessary for disease control and reduction of economic loss. in the present study, a reverse transcriptase loop-mediated isothermal amplification (rt-lamp) assay for the rapid and sensitive detection of ssrv was developed and evaluated. the lamp ... | 2011 | 21945219 |
| identification and characterization of a serine protease inhibitor (ptserpin) in the swimming crab portunus trituberculatus. | serine protease inhibitors (serpins) play a key role in diverse immune biological processes. a serine protease inhibitor (serpin), namely ptserpin, was identified from the haemocyte cdna library of swimming crab portunus trituberculatus. the full-length ptserpin cdna was 1593 bp, including an open reading frame (orf) of 1227 bp encoding a polypeptide of 408 amino acids with estimated molecular mass of 45.048 kda and theoretical isoelectric point of 7.23. predicted tertiary structure of ptserpin ... | 2012 | 22245590 |
| a novel myeloid differentiation factor 88 homolog, spmyd88, exhibiting sptoll-binding activity in the mud crab scylla paramamosain. | myeloid differentiation factor 88 (myd88) is an essential regulator in the toll or toll-like receptor (tlr) signaling pathway. in the current study, we characterized a novel crustacean myd88 homolog, spmyd88, and analyzed its binding activity with sptoll. the full-length cdna sequence of spmyd88 is 2933 bp, with a 1419 bp open reading frame encoding a 472-amino acid protein. no signal peptide was predicted. a death domain (residues 19-103), a toll/interleukin-1 receptor (tir) domain (residues 15 ... | 2012 | 23280154 |
| feasibility study on the culture of mud crab scylla serrata in the mid coast region of bangladesh. | a study was conducted at hatiya and nijhum island, noakhali in the mid coastal region of bangladesh to identify feasibility of mud crab culture for a study period of six months from january 2010 to june 2010. data were collected through questionnaire interviews, focus group discussions and cross check interviews. average yearly collection of mud crabs in this area was about 890.36 tons where lower grade crabs 45.16% and these can be brought under culture practice. it has observed that saline wat ... | 2012 | 23755411 |
| biology and conservation of the genus scylla in india subcontinent. | mud crabs usually inhabit brackish water bodies of south-east asia including india. the biological significance of the species is its survibility in wide range of hydro-biological fluctuating environments including salinity. the life cycle of the species includes two important larval stages at which they are vulnerable to various predators. pathophysiological status of the species is a challenge for its aquaculture. inspite of a huge economical and high food values, informations on biochemical, ... | 2012 | 23734453 |
| copper toxicity in the crab, scylla serrata, copper levels in tissues and regulation after exposure to a copper-rich medium. | | 2013 | 3689997 |
| production of methyl farnesoate by the mandibular organs of the mud crab, scylla serrata: validation of a radiochemical assay. | parameters for the validation of a radiochemical assay to monitor the biosynthesis and release of methyl farnesoate (mf) by the mandibular organs (mo) of the mud crab, scylla serrata, have been defined. on the basis of hplc analysis, mf appeared to be the exclusive radiolabeled product of release, using [3h-methyl]methionine as precursor. hplc of isooctane extracts of medium in which mo had been maintained similarly revealed that mf was the exclusive 3h-product and indicates that the isooctane p ... | 2010 | 2925075 |
| binding of oxygen and carbon monoxide to arthropod hemocyanin: an allosteric analysis. | the binding of oxygen and carbon monoxide to hemocyanin from the mangrove crab scylla serrata and the lobster homarus americanus has been studied by thin-layer optical absorption and front face fluorescence techniques. three types of experiments were performed on subunit and oligomeric preparations of each hemocyanin: oxygen binding, carbon monoxide binding, and oxygen-carbon monoxide competition studies. the results obtained from the subunit preparations of dissociated oligomers from both hemoc ... | 2014 | 3994961 |
| the new antimicrobial peptide sphyastatin from the mud crab scylla paramamosain with multiple antimicrobial mechanisms and high effect on bacterial infection. | sphyastatin was first identified as a new cationic antimicrobial peptide in hemocytes of the mud crab scylla paramamosain. based on the amino acid sequences deduced, it was predicted that this peptide was composed of two different functional domains, a proline-rich domain (prd) and a cysteine-rich domain (crd). the recombinant product of sphyastatin displayed potent antimicrobial activities against the human pathogen staphylococcus aureus and the aquatic animal pathogens aeromonas hydrophila and ... | 2016 | 27493644 |
| identification of a serine proteinase homolog (sp-sph) involved in immune defense in the mud crab scylla paramamosain. | clip domain serine proteinase homologs are involved in many biological processes including immune response. to identify the immune function of a serine proteinase homolog (sp-sph), originally isolated from hemocytes of the mud crab, scylla paramamosain, the sp-sph was expressed recombinantly and purified for further studies. it was found that the sp-sph protein could bind to a number of bacteria (including aeromonas hydrophila, escherichia coli, staphylococcus aureus, vibrio fluvialis, vibrio ha ... | 2013 | 23724001 |
| identification of genes associated with reproduction in the mud crab (scylla olivacea) and their differential expression following serotonin stimulation. | the central nervous system (cns) is often intimately involved in reproduction control and is therefore a target organ for transcriptomic investigations to identify reproduction-associated genes. in this study, 454 transcriptome sequencing was performed on pooled brain and ventral nerve cord of the female mud crab (scylla olivacea) following serotonin injection (5 µg/g bw). a total of 197,468 sequence reads was obtained with an average length of 828 bp. approximately 38.7% of 2,183 isotigs matche ... | 2014 | 25542017 |
| neuropeptides in the cerebral ganglia of the mud crab, scylla paramamosain: transcriptomic analysis and expression profiles during vitellogenesis. | neuropeptides play a critical role in regulating animal reproduction. in vertebrates, gnrh, gnih and kisspeptin are the key neuropeptide hormones of the reproductive axis, however, the reproductive axis for invertebrates is vague. knowledge on ovarian development of the mud crab, scylla paramamosain, is critical for aquaculture and resources management of the commercially important species. this study employed illumina sequencing, reverse transcription-polymerase chain reaction and quantitative ... | 2015 | 26592767 |
| functional assessment of residues in the amino- and carboxyl-termini of crustacean hyperglycemic hormone (chh) in the mud crab scylla olivacea using point-mutated peptides. | to assess functional importance of the residues in the amino- and carboxyl-termini of crustacean hyperglycemic hormone in the mud crab scylla olivacea (sco-chh), both wild-type and point-mutated chh peptides were produced with an amidated c-terminal end. spectral analyses of circular dichroism, chromatographic retention time, and mass spectrometric analysis of the recombinant peptides indicate that they were close in conformation to native chh and were produced with the intended substitutions. t ... | 2015 | 26261986 |
| the retinoid x receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway. | in arthropods, retinoid x receptor (rxr) is a highly conserved nuclear hormone receptor. by forming a heterodimeric complex with the ecdysone receptor (ecr), rxr is known to be vital importance for various physiological processes. however, in comparison to ecr, the rxr signaling pathway and its roles in crustacean reproduction are poorly understood. in the present study, the rxr mrna was detected in the ovarian follicular cells of mud crab scylla paramamosain (sprxr) and during ovarian maturatio ... | 2016 | 27009370 |
| identification, characterization, and functional analysis of tube and pelle homologs in the mud crab scylla paramamosain. | tube and pelle are essential components in drosophila toll signaling pathway. in this study, we characterized a pair of crustacean homologs of tube and pelle in scylla paramamosain, namely, sptube and sppelle, and analyzed their immune functions. the full-length cdna of sptube had 2052 bp with a 1578 bp open reading frame (orf) encoding a protein with 525 aa. a death domain (dd) and a kinase domain were predicted in the deduced protein. the full-length cdna of sppelle had 3825 bp with a 3420 bp ... | 2013 | 24116143 |