identification of species in cooked crabmeat by thin layer isoelectric focusing.thin layer polyacrylamide gel isoelectric focusing (tlief) is described for characterizing the species-specific, heat-denatured proteins of 8 species of crab: red (geryon quinquedens), rock (cancer irroratus), jonah (cancer borealis), blue (callinectes sapidus), king (paralithodes camtschatica), snow (chionoectes spp.), european edible (cancer pagurus), and dungeness (cancer magister). protein pattern differences are shown not only among species, but also between 2 modes of heat processing of th ...197939059
mass spectrometric identification of pegfysqryamide: a crustacean peptide hormone possessing a vertebrate neuropeptide y (npy)-like invertebrates, peptides possessing the carboxy (c)-terminal motif -rxrfamide have been proposed as the homologs of vertebrate neuropeptide y (npy). using matrix assisted laser desorption/ionization mass spectrometry, in combination with sustained off-resonance irradiation collision-induced dissociation and chemical and enzymatic reactions, we have identified the peptide pegfysqryamide from the neuroendocrine pericardial organ (po) of the crab pugettia producta. this peptide is likely the same ...200717420018
identification, physiological actions, and distribution of tpsgflgmramide: a novel tachykinin-related peptide from the midgut and stomatogastric nervous system of cancer most invertebrates, multiple species-specific isoforms of tachykinin-related peptide (trp) are common. in contrast, only a single conserved trp isoform, apsgflgmramide, has been documented in decapod crustaceans, leading to the hypothesis that it is the sole trp present in this arthropod order. previous studies of crustacean trps have focused on neuronal tissue, but the recent demonstration of trps in midgut epithelial cells in cancer species led us to question whether other trps are present ...200717437551
serotonergic/cholinergic muscle receptor cells in the crab stomatogastric nervous system. i. identification and characterization of the gastropyloric receptor cells.1. serotonin (5-hydroxytryptamine) immunohistochemistry was used to locate and anatomically describe a set of four muscle receptor cells in the stomatogastric nervous system of the crabs cancer borealis and cancer irroratus. we found that these sensory cells, which we named gastropyloric receptor (gpr) cells, are the sole source of serotonergic inputs to the stomatogastric ganglion (stg) in these species. thus any endogenous serotonergic modulation of the central pattern generators (cpgs) in the ...20092769347
[metabolism of the fe, cu, zn, mg, mn and co in the ovum of cancer irroratus (decapod crustacean)].the concentrations of fe, cu, zn, mn and co in the eggs of cancer irroratus show variations in relation with time running out after laying, that is to say with embryogenesis. the increase of the rates of fe, mn and mg is independant of the variations of the water content. inversely, the decrease of cu and zn concentrations is in close relation with the increase of water content during the same period. the increase of fe, mn and mg concentrations seems to be essentially under the dependance of ph ...2002134767
members of the crustacean hyperglycemic hormone (chh) peptide family are differentially distributed both between and within the neuroendocrine organs of cancer crabs: implications for differential release and pleiotropic function.the crustacean hyperglycemic hormone (chh) family of peptides includes chh, moult-inhibiting hormone (mih) and mandibular organ-inhibiting hormone (moih). in the crab cancer pagurus, isoforms of these peptides, as well as chh precursor-related peptide (cprp), have been identified in the x-organ-sinus gland (xo-sg) system. using peptides isolated from the c. pagurus sg, antibodies to each family member and cprp were generated. these sera were then used to map the distributions and co-localization ...200616888072
pah transport in rock crab (cancer irroratus) urinary bladder.crab urinary bladder appears to possess several morphological and functional similarities to vertebrate renal proximal tubule. sections of intermolt rock crab bladder accumulated pah by a process that was concentrative (60 min tissue-to-medium ratio (t/m) for 10 microm pah averaged 24), na dependent, powered by glycolytic metabolism, and inhibitable by other organic anions. initial section uptakes exhibited saturation kinetics and a double-reciprocal plot of uptake vs. concentration yielded a si ...19807377371
neuroendocrine regulation of carbonic anhydrase expression in the gills of the euryhaline green crab, carcinus maenas.the relationship between branchial carbonic anhydrase (ca) activity, ca gene expression and salinity, and potential mechanisms of regulation, was investigated in the euryhaline green crab, carcinus maenas, acclimated to 33 ppt and transferred to 10 ppt, and the stenohaline rock crab, cancer irroratus, acclimated to 32 ppt and transferred to 18 ppt. ca activity in green crabs acclimated to high and low salinity was a function of ca mrna expression, with low salinity exposure resulting in an incre ...200616788899
biogeographic variation in behavioral and morphological responses to predation risk.the expression of prey antipredator defenses is often related to ambient consumer pressure, and prey express greater defenses under intense consumer pressure. predation is generally greater at lower latitudes, and antipredator defenses often display a biogeographic pattern. predation pressure may also vary significantly between habitats within latitudes, making biogeographic patterns difficult to distinguish. furthermore, invasive predators may also influence the expression of prey defenses in e ...201323001623
physiological responses to digestion in low salinity in the crabs carcinus maenas and cancer irroratus.osmoregulation and digestion are energetically demanding, and crabs that move into low salinity environments to feed must be able to balance the demands of both processes. achieving this balance may pose greater challenges for weak than for efficient osmoregulators. this study examined the rate of oxygen consumption (mo2) of carcinus maenas (efficient osmoregulator) and cancer irroratus (weak osmoregulator) as a function of feeding and hyposaline stress. the mo2 increased 2-fold in both species ...201626459987
effect of meal size and body size on specific dynamic action and gastric processing in decapod crustaceans.meal size and animal size are important factors affecting the characteristics of the specific dynamic action (sda) response across a variety of taxa. the effects of these two variables on the sda of decapod crustaceans are based on just a couple of articles, and are not wholly consistent with the responses reported for other aquatic ectotherms. therefore, the effects of meal size and animal size on the characteristics of sda response were investigated in a variety of decapod crustaceans from dif ...201323916818
attraction of predatorily naive postlarval lobsters to extracts of metabolites of common prey:mytilus edulis, mya arenaria, cancer irroratus, andasterias vulgaris.postlarval lobsters (4th-7th stage) exclusively fed frozen brine shrimp (artemia saline) were assayed for food-search response to extracts and metabolites from four common prey: soft clams (mya arenaria), blue mussels (mytilus edulis), rock crabs (cancer irroratus), and sea stars (asterias vulgaris). concentrations of soluble primary amines, protein, and ammonia in prey tissues and metabolites were determined. no significant responses were observed for any prey metabolites diluted to 1 and 10%, ...198724302143
subtidal food thieves: interactions of four invertebrate kleptoparasites with the sea star leptasterias polaris.we used time-lapse underwater video systems in the subtidal zone of the mingan islands, eastern canada, to examine behavioural interactions of four kleptoparasites with the sea star leptasterias polaris when it was feeding on infaunal (buried) clams. departures of l. polaris from its prey coincided with interactions with kleptoparasites in 10 out of 10 filmed feeding bouts on the large clam spisula polynyma, compared with only four out of 10 of filmed bouts on the smaller clam mya truncata. the ...200011032656
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