Publications

TitleAbstractYear
Filter
PMID
Filter
identification of species in cooked crabmeat by thin layer isoelectric focusing.thin layer polyacrylamide gel isoelectric focusing (tlief) is described for characterizing the species-specific, heat-denatured proteins of 8 species of crab: red (geryon quinquedens), rock (cancer irroratus), jonah (cancer borealis), blue (callinectes sapidus), king (paralithodes camtschatica), snow (chionoectes spp.), european edible (cancer pagurus), and dungeness (cancer magister). protein pattern differences are shown not only among species, but also between 2 modes of heat processing of th ...197939059
the specificity of a neutral deoxyribonuclease from cancer pagurus.the recently isolated neutral deoxyribonuclease from crab (cancer pagurus) testes has been characterized in its mode of action and its specificity. the enzyme is a typical endonuclease, forming 5'-phosphate oligonucleotides of large average size; after extensive digestion of calf thymus dna over 75% of the fragments have a size larger than pentanucleotides and mononucleotides are absent. as far as specificity is concerned, thymidine is very abundant in the 5'-penultimate position (approximately ...19751236141
spectroscopic and chemical approaches to the study of metal-thiolate clusters in metallothionein (mt).structural properties of the metal-thiolate clusters in mammalian and vertebrate mts were studied by employing various spectroscopic techniques. the stepwise probing of the metal-binding sites of the four-metal cluster domain (alpha-fragment) of rabbit mt-2 by co(ii) ions monitoring the v2[4a2----4t1(p)] ligand-field transition at 1260 nm and the epr intensity of the high spin co(ii) complexes revealed spectral changes suggestive of a cluster structure with one metal-binding site of different sy ...19872822462
nmr studies of crab and plaice metallothioneins.metallothioneins isolated from the hepatopancreas of the edible crab (cancer paqurus) and the plaice (pleuronectes platessa) after cadmium injection are predominantly cadmium proteins containing only small amounts of zinc and traces of copper. the removal of metal ions from the two metallothioneins by edta was studied using proton nmr spectroscopy. the rates of removal of cadmium and zinc were monitored directly from the intensity of the resonances due to the cadmium and zinc-edta complexes. nea ...19863086076
occurrence of cadmium in crabs (cancer pagurus) and the isolation and properties of cadmium metallothionein.the occurrence of high cadmium levels in the digestive gland (hepatopancreas) of the crab cancer pagurus is reviewed and its possible significance to human health is discussed. previous attempts to isolate this cadmium in its native form are briefly reviewed and the present method used in this laboratory to isolate crab cadmium metallothionein is described. quantitative amino acid analysis of the purified protein suggests that it has a total of 55 residues, including 19 cys, and a molecular weig ...19863709430
studies on phosphagen synthesis by mitochondrial preparations.mitochondrial preparations from muscles of a crab (cancer pagurus), two fish (trachurus trachurus and scyliorhinus canicula) and a bird (columba livia) are able to synthesise, through atp, the phosphagen related to that species. this indicates the presence of a bound phosphagen kinase. addition of creatine kinase and creatine to crab mitochondria results in the synthesis of phosphocreatine. similarly, the addition of arginine kinase and arginine to mitochondrial preparations from the fish and bi ...19854006444
isolation and characterization of a neutral deoxyribonuclease from the testes of the crab cancer pagurus. 19744366943
proceedings: presence of a natural heteroagglutinin in the serum of the crab cancer pagurus. 19744461483
uptake of bacteriophage and their subsequent survival in edible west coast crabs after processing.edible west coast crabs (cancer magister and cancer antennarius), when in seawater contaminated with coliphage t4, were found to accumulate high titers of this virus. to study the extent of the hazard presented by crabs contaminated with virus, samples of edible crabs were contaminated with coliphage t4 and then processed by boiling. results indicated that virus in crabs withstood this method of processing. the survival rate varied from 2.5 to 20%, depending upon the processing time. heat penetr ...19725064986
bacteria associated with crabs from cold waters with emphasis on the occurrence of potential human pathogens.a diverse array of bacterial species, including several potential human pathogens, was isolated from edible crabs collected in cold waters. crabs collected near kodiak island, alaska, contained higher levels of bacteria than crabs collected away from regions of human habitation. the bacteria associated with the crabs collected near kodiak included yersinia enterocolitica, klebsiella pneumoniae, and coagulase-negative staphylococcus species; the pathogenicity of these isolates was demonstrated in ...19846742824
pah transport in rock crab urinary bladder. ii. luminal and serosal steps.to characterize the organic anion transport properties of the luminal (1) and serosal (s) membranes of crab urinary bladder, initial (10 min) fluxes (j) and tissue accumulations (ac) of 10 microm pah were measured in the absence and presence of 1 mm bcg (nontransported, high-affinity competitor). control bladders exhibited net reabsorptive transport with a mean jl leads to s/js leads to 1 of about 7; concentrative transport of p-aminohippuric acid (pah) occurred only across the luminal cell (c) ...19827058926
pah transport in rock crab (cancer irroratus) urinary bladder.crab urinary bladder appears to possess several morphological and functional similarities to vertebrate renal proximal tubule. sections of intermolt rock crab bladder accumulated pah by a process that was concentrative (60 min tissue-to-medium ratio (t/m) for 10 microm pah averaged 24), na dependent, powered by glycolytic metabolism, and inhibitable by other organic anions. initial section uptakes exhibited saturation kinetics and a double-reciprocal plot of uptake vs. concentration yielded a si ...19807377371
bioavailability of cadmium from crab hepatopancreas and mushroom in relation to inorganic cadmium: a 9-week feeding study in mice.the bioavailability of cd from boiled crab hepatopancreas and dried mushroom was studied in relation to that of inorganic cd (cdcl2). female balb/c mice were fed with diets containing 0.4 ppm cd from either boiled crab (cancer pagurus) hepatopancreas or dried mushroom (agaricus augustus), or as inorganic cd (cdcl2). a control group received low cd (< 0.007 ppm) feed, and did not accumulate detectable levels of cd during 9 wk of exposure. using cd accumulation in the liver and kidney as a measure ...19957672739
actin in the acrosome of the spermatozoa of the crab, cancer pagurus l. (decapoda, crustacea).the cytoskeletal protein actin was identified in the mature spermatozoon of the european edible crab, cancer pagurus linnaeus, by indirect immunofluorescence with monoclonal and polyclonal anti-actin antibodies, fluorescent phalloidin, and dnaase i. the actin was localized in two distinct concentric rings within the acrosome vesicle of the spermatozoon and appeared to correlate with the internal zonation of the vesicle. modifications of the fluorescent pattern for actin were observed in sperm ce ...19948080647
inhibition of bacterial respiration by a low-molecular weight lectin, scyllin, from scylla serrata crab hemolymph.interaction of plant and/or invertebrate lectins with mammalian cells and different microorganisms is well known. in the present study, we have demonstrated that scyllin, a low molecular weight (mw 4000) lectin from the edible crab scylla serrata hemolymph, purified by galnac-sepharon affinity column followed by mono-q ion exchanger in fplc exhibits antimicrobial activity against bacillus cereus and escherichia coli by inhibiting endogenous respiration as well as exogenous glucose oxidation. in ...19979343934
influence of naphthalene on protein, carbohydrate, and phosphatases system during the vitellogenesis in marine edible crab, scylla serrata. 19989484552
digital electromagnetic telemetry system for studying behaviour of decapod crustaceans.1 year and the receiving system batteries are replaced by divers at intervals of up to 4 weeks. in field tests, crab (cancer pagurus l.) and lobster (homarus gammarus (l.)) activity was monitored at an artificial reef for 14 months. examples of the type of information acquired are presented to illustrate the capabilities of the system and potential applications are discussed. limitations of digital electromagnetic telemetry stem mainly from the short range of detection, the need for cables on th ...200010742505
deoxyribonucleic acid of cancer pagurus. ii. template activity for a dna-dependent dna polymerase of eukaryotic cells.the template activity of cancer pagurus dna and its two components (poly d(a-t) and main component) in response to a dna polymerase purified from regenerating rat liver has been studied and compared to the results previously obtained with synthetic templates. in the double-stranded native state, whole crab dna and the main component were poor templates. their replication was increased by thermal denaturation and inhibited by actinomycin. like the synthetic copolymer poly[d(a-t).d(t-a)], native c ...197410793685
characterization and sequence elucidation of a novel peptide with molt-inhibiting activity from the south african spiny lobster, jasus lalandii.we have isolated a peptide from extracts of sinus glands from a south african spiny lobster species, jasus lalandii, by high-performance liquid chromatography (hplc) and identified it as a putative molt-inhibiting hormone (mih) by (i) an in vitro assay with j. lalandii y-organs to measure the inhibition of ecdysteroid synthesis and (ii) an immunoassay using antiserum raised against mih of the edible crab. the mih of j. lalandii has 74 amino acid residues, a molecular mass of 9006 da, a free n-te ...200011072117
variability in the edible fraction content of 60co, 99tc, 110mag, 137cs and 241am between individual crabs and lobsters from sellafield (north eastern irish sea).we investigated the variability of anthropogenic radionuclide content of the edible fractions of individual edible crabs (cancer pagurus l.) and european lobsters (homarus gammarus l.) caught commercially in the sellafield offshore area. sixteen female and 18 male crabs and 20 female and 17 male lobsters were selected from commercial catches made between 25 may and 5 june 1997. each gender group was selected to be within the known weight range for commercially caught crustacea from the area. fou ...200111381940
a histological study of shell disease syndrome in the edible crab cancer pagurus.shell disease syndrome is characterised by the external manifestation of black spot lesions in the exoskeletons of crustaceans. in the present study, gills, hepatopancreas and hearts from healthy (<0.05% black spot coverage) and diseased (5 to 15% coverage) edible crabs, cancer pagurus, were examined histologically to determine whether this disease can cause internal damage to such crabs. there was clear evidence of cuticular damage in the gills of diseased crabs leading to the formation of haem ...200111804420
shell disease syndrome in the edible crab, cancer pagurus--isolation, characterization and pathogenicity of chitinolytic bacteria.chitinolytic bacteria are believed to be the primary aetiological agents of shell disease syndrome in marine crustaceans. the disease principally results from the breakdown of their chitinous exoskeletons by the shell disease pathogens, but pathogenicity may also manifest internally should a breach of the carapace occur. the current study looks at the pathogenicity of a number of bacteria (predominantly from the genus vibrio) isolated from the edible crab, cancer pagurus. all chitinase-producing ...200211882709
dynamics of shell disease in the edible crab cancer pagurus: a comparative study between two sites on the gower peninsula, south wales, uk.shell disease syndrome is a degradative condition of the crustacean exoskeleton which results in the formation of black-spot lesions. field surveys on the edible crab cancer pagurus (l.) population at 2 sites in the gower peninsula of south wales, uk, namely langland bay and rhossili causeway, revealed similarities in the prevalence of shell disease but differences in the severity. male crabs from langland bay displayed significantly greater severities of the disease on the dorsal carapace and v ...200212542092
co-infection by a yeast-like organism in hematodinium-infected european edible crabs cancer pagurus and velvet swimming crabs necora puber from the english channel.during the winter months, edible crabs cancer pagurus and velvet swimming crabs necora puber from the english channel can harbour infections by a hematodinium sp. dinoflagellate. this parasite is responsible for a highly pathological condition known as 'pink crab disease' (pcd) in the edible crab. in the current study, a high proportion (between 25 and 100%) of hematodinium-infected edible and velvet swimming crabs captured from 2 sites in the english channel also harboured a systemic infection ...200312803383
immunocytochemical and molecular data guide peptide identification by mass spectrometry: orcokinin and orcomyotropin-related peptides in the stomatogastric nervous system of several crustacean species.in order to identify new orcokinin and orcomyotropin-related peptides in crustaceans, molecular and immunocytochemical data were combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). in the crayfish procambarus clarkii, four orcokinins and an orcomyotropin-related peptide are present on the precursor. because these peptides are highly conserved, we assumed that other species have an identical number of peptides. to identify the peptides, immun ...200314528921
cloning, characterization, and developmental expression of a putative farnesoic acid o-methyl transferase in the female edible crab cancer pagurus.farnesoic acid methyl transferase (famtase) catalyzes methylation of farnesoic acid to yield the crustacean juvenoid, methyl farnesoate (mf). a full-length cdna encoding a 275 amino acid putative famtase has been isolated from the mandibular organ of the female edible crab (cancer pagurus) by reverse transcriptase-polymerase chain reaction in conjunction with cdna library screening. a high degree of sequence identity was found between this and other putative crustacean famtases. conceptual trans ...200314672985
effect of extracellular products of pseudoalteromonas atlantica on the edible crab cancer pagurus.previous studies have shown that injection of extracellular products (ecp) of pseudoalteromononas atlantica isolated from shell disease-infected edible crabs (cancer pagurus) into healthy crabs causes rapid death. in this study we examined the nature of the active lethal factor(s) in ecp. injection of ecp into crabs caused a rapid decline in the total number of circulating hemocytes (blood cells), and the crabs died within 60 to 90 min. the individuals that died showed eyestalk retraction, limb ...200414766548
the influence of natural surface microtopographies on fouling.multiple antifouling strategies of marine organisms may consist of combinations of physical, chemical and mechanical mechanisms. in this study, the role of surface microtopography (< 500 microns) of different marine organisms, such as cancer pagurus, mytilus edulis, ophiura texturata and the eggcase of scyliorhinus canicula, has been investigated as a possible component of their defence systems. high resolution resin replicates of these natural surface structures were exposed to natural fouling ...200415079892
dietary preference and digestive enzyme activities as indicators of trophic resource utilization by six species of crab.the digestive physiology and stomach contents of six crab species from a variety of habitats were investigated to provide an indication of their digestive capability and dietary preferences. stomach contents varied between species, but the key enzymes present were generally consistent with the types of dietary material being ingested. nectocarcinus integrifons (red rock crab) consumed large quantities of seagrass and had high cellulase activity (0.02+/-0.004 units mg-1) to digest the constituent ...200515713811
four differentially expressed cdnas in callinectes sapidus containing the rebers-riddiford consensus sequence.decapod crustaceans such as callinectes sapidus, the blue crab, provide unique opportunities to study proteins involved in biomineralization. subsequent to each molt, the previously deposited soft cuticle is calcified while the postecdysial layers are simultaneously deposited and mineralized. though the majority of the exoskeleton hardens, morphologically similar cuticle at the joints, called arthrodial membrane, remains flexible. it seems reasonable that hypodermal cells producing these cuticle ...200515939643
diarrhetic shellfish poisoning by okadaic acid esters from brown crabs (cancer pagurus) in norway.in 2002 several hundred people were taken ill after eating self-harvested brown crabs (cancer pagurus) in the southern part of norway. the symptoms were similar to diarrhetic shellfish poisoning (dsp) although with a somewhat delayed onset. this happened at the same time as an unusual early bloom of dinophysis acuta had lead to high amounts of dsp toxins in blue mussels (mytilus edulis) in the same area. the proposed cause of the intoxication was that crabs had accumulated toxins by eating blue ...200516153454
unchanged prevalence of shell disease in the edible crab cancer pagurus four years after decommissioning of a sewage outfall at langland bay, uk.shell disease is a bacteria-associated degradative disease of the crustacean exoskeleton that leads to formation of extensive lesions in the cuticle with ultimate involvement of haemocoelic septicaemia. field surveys of edible crabs cancer pagurus from langland bay, gower, uk, from february 1997 to march 1998 had revealed unusually high prevalence (ca. 55%) of this disease amongst the population, and it was suggested that one of the predisposing factors might have been the presence of raw sewage ...200516465837
aspartic proteinases in the digestive tract of marine decapod crustaceans.decapod crustaceans synthesize highly active proteolytic enzymes in the midgut gland and release at least a part of them into the stomach where they facilitate the first step in peptide hydrolysis. the most common proteinases in the gastric fluid characterized so far are serine proteinases, that is, trypsin and chymotrypsin. these enzymes show highest activities at neutral or slightly alkaline conditions. the presence of acid proteinases, as they prevail in vertebrates, has been discussed contra ...200616788916
members of the crustacean hyperglycemic hormone (chh) peptide family are differentially distributed both between and within the neuroendocrine organs of cancer crabs: implications for differential release and pleiotropic function.the crustacean hyperglycemic hormone (chh) family of peptides includes chh, moult-inhibiting hormone (mih) and mandibular organ-inhibiting hormone (moih). in the crab cancer pagurus, isoforms of these peptides, as well as chh precursor-related peptide (cprp), have been identified in the x-organ-sinus gland (xo-sg) system. using peptides isolated from the c. pagurus sg, antibodies to each family member and cprp were generated. these sera were then used to map the distributions and co-localization ...200616888072
organochlorinated contaminants in decapod crustaceans from the coasts of brittany and normandy (france).the contamination and distribution of organochlorinated compounds were considered in three crustacean species (edible crab, cancer pagurus; spider crab, maja brachydactyla; velvet swimming crab, necora puber) from five sites along the coasts of brittany and normandy (western and north-western france). pcbs (16 single congeners), pp'-dde and hcb were measured in hepatopancreas, gonads and muscle: in all, 175 samples were analysed. the spider crab was the only species found in the five sampling si ...200717223177
molecular characterization and cell-specific expression of an ion transport peptide in the tobacco hornworm, manduca sexta.the crustacean hyperglycemic hormone (chh) peptides regulate diverse physiological processes from reproduction to metabolism and molting in arthropods. in insects, the ion transport peptides (itp), also members of the chh family, have only been implicated in ion transport. in this study, we sequenced a nucleotide fragment spanning the conserved a1/a2 region of the putative chh/itp gene. this fragment was amplified from larval cdna of the tobacco hornworm, manduca sexta and showed a high degree o ...200717450383
enterospora canceri n. gen., n. sp., intranuclear within the hepatopancreatocytes of the european edible crab cancer pagurus.only 1 genus (nucleospora) within 1 family (enterocytozoonidae) of the microsporidia contains species that are parasitic within the nuclei of their host cells; to date, all described intranuclear nucleospora spp. parasitise fish. this study describes the first intranuclear microsporidian parasite of an invertebrate, the european edible crab cancer pagurus l. (decapoda: cancridae). infected crabs displayed no obvious external signs, and maximum apparent prevalence of infection within a monthly sa ...200717523544
conservation in the first internal transcribed spacer region (its1) in hematodinium species infecting crustacean hosts found in the uk and newfoundland.parasitic dinoflagellates in the genus hematodinium infect a number of decapod crustaceans in waters off the uk, including the norway lobster nephrops norvegicus and the edible crab cancer pagurus. this study investigated sequence variability in the first internal transcribed spacer (its1) region of the ribosomal rna complex of hematodinium spp. infecting n. norvegicus, c. pagurus, and pagurus bernhardus from 4 locations in the uk and from the hematodinium sp. infecting chionoecetes opilio from ...200717629120
structural confirmation and occurrence of azaspiracids in scandinavian brown crabs (cancer pagurus).in 2005 and 2006, azaspiracids were for the first time detected in brown crabs (cancer pagurus) from the west coast of sweden and the north and north-west coast of norway. azaspiracids are marine toxins that have been detected in blue mussels in europe in recent years. on some occasions, they have been responsible for human intoxications with symptoms similar to those occurring by consumption of shellfish contaminated with okadaic acid group toxins. while the latter toxin group has been reported ...200817936866
molecular cloning of four cdnas encoding prepro-crustacean hyperglycemic hormone (chh) from the eyestalk of the red rock crab cancer productus: identification of two genetically encoded chh isoforms and two putative post-translationally derived chh variants.recently, we demonstrated that the four known sinus gland (sg) isoforms of cancer productus crustacean hyperglycemic hormone precursor-related peptide (capr-cprp i-iv) are differentially distributed in conserved patterns among individual crabs. this finding strongly supported the presence of multiple prepro-crustacean hyperglycemic hormone (chh) transcripts in each crab, as well as the translation and processing of the encoded prepro-hormones. whether these transcripts contained common or distin ...200817961562
the human alimentary tract transfer and body retention of environmental polonium-210.this paper presents the results of a 4 year study to investigate the human alimentary tract transfer factor (f(a) value) and body retention of 210 po in shellfish. in the first 3 years, mussels (mytilus edulis l.), cockles (cerastoderma edule l.) and brown meat from crab (cancer pagurus l.) were successively studied. in each year five volunteers (from a pool of seven) ate a suitable portion of the shellfish and provided 24 h samples of excreta usually for 3 days before and for at least 7 days du ...200718268372
short- and long-term dietary effects on disease and mortality in american lobster homarus americanus.the american lobster homarus americanus fishery is heavily dependent on the use of fish as bait to entice lobsters into traps. there is concern that this food supplementation is nutritionally insufficient for lobsters, but previous experiments reported conflicting results. we conducted a long-term feeding experiment in which 1 yr old american lobsters were fed one of 7 diets for a period of 352 d, a time that allowed the lobsters to molt thrice. the diets consisted of fresh frozen herring, a 'wi ...200818380224
cancer pagurus bacilliform virus (cpbv) infecting juvenile european edible crabs c. pagurus from uk waters.this study provides the first description of an intranuclear bacilliform virus infecting the hepatopancreatic epithelial cells of juvenile european edible crab cancer pagurus from the english channel, uk. this is the first field report of a virus infecting a member of the cancer genus and follows a report of a different virus infection detected in c. pagurus held under experimental conditions. we have named the virus cancer pagurus bacilliform virus (cpbv). the morphology and size of the nucleoc ...200818500031
experimental evidence for latent developmental plasticity: intertidal whelks respond to a native but not an introduced predator.animals with highly inducible traits may show no inducible response when exposed to a related but wholly novel cue. this appears to be true for the intertidal whelk nucella lamellosa faced with a voracious introduced predator. in the laboratory, we exposed whelks to effluent from two species of predatory crab, the native red rock crab cancer productus and the invasive european green crab carcinus maenas. nucella and cancer have a long shared history in the northeast pacific, whereas potential in ...200818522925
a c-type lectin like-domain (ctld)-containing protein (ptlp) from the swimming crab portunus trituberculatus.c-type lectins play important roles in the non-self innate immune system of invertebrates. in this study, we isolated the full-length cdna of the c-type lectin like-domain (ctld)-containing protein, designated ptlp, from the hepatopancreas of the swimming crab portunus trituberculatus, one of the most common edible crabs of east asia. the ptlp cdna consists of 923 bp and encodes a polypeptide of 164 amino acids containing a well-conserved c-type lectin like-domain (ctld). the deduced amino acid ...200818603446
skin test for paragonimiasis among schoolchildren and villagers in namback district, luangprabang province, lao pdr.as a part of a broader effort to determine the status of paragonimus species infection in lao pdr, an epidemiological survey was conducted on villagers and schoolchildren in namback district between 2003 and 2005. among 308 villagers and 633 primary and secondary schoolchildren, 156 villagers and 92 children evidenced a positive reaction on a paragonimus skin test. only 4 schoolchildren out of 128 skin test-positive cases had paragonimus sp. eggs in their sputum, all of which was collected on 1 ...200818830059
experiments on the carbon dioxide transport by the blood of the edible snail (helix pomotial) the common crab (cancer pagurus l.) and the common lobster (homarus vulgaris m. e.). 194818874549
development of primary cell culture from scylla serrata : primary cell cultures from scylla serrata.this paper reports for the first time, the primary cell culture of hepatopancreas from edible crab scylla serrata using crab saline, l-15 (leibovitz), 1 x l-15 + crab saline, 2 x l-15 + crab saline, 3 x l-15 and citrate buffer without any serum. we could isolate and maintain e (embryonalzellen), f (fibrenzellen), b (blasenzellen), r (restzellen) and g (granular cells). upon seeding the hepatopancreatic e, f, b, and r cells showed different survival pattern over time than granular cells. a modifi ...200819002854
accumulation of elements (s, as, br, sr, cd, hg, pb) in two populations of cancer pagurus: ecological implications to human consumption.the brown crab cancer pagurus is highly appreciated in southern european countries and edible tissues are consumed separately or as mixtures. this species is mostly harvested along the scottish coast and english channel and has different market prices depending on the catching area and sex. the aim of this study was to quantify and characterize the contents of s, as, br, sr, cd, hg and pb in muscle, hepatopancreas, gonads and gills of female and male crabs from both catching areas. additionally, ...200919010376
influence of season and sex on the contents of minerals and trace elements in brown crab (cancer pagurus, linnaeus, 1758).cancer pagurus is much appreciated in southern europe, where the muscle, hepatopancreas, and gonads are consumed regularly with peaks in summer and december. the elemental contents of c. pagurus edible tissues were analyzed in this study during the four seasons. results indicate that the content varied with tissue, season, and sex. the hepatopancreas had more s, cl, ca, br, sr, fe, cu, cd, and pb, the gonads had a higher concentration of na, and the muscle was richer in zn. autumn and winter cor ...200919368352
metals in marine environment (mollusc patella sp., fish labrus bergylta, crustacean cancer pagurus, beach sand) in a nuclear area, the north cotentin (france).the results of a 1-year long survey of trace metals concentrations (al, cd, cr, co, cu, fe, mn, hg, ni, pb, zn) measured in beach sand, limpets and, occasionally, in fish and shellfish from the north cotentin area (france), where nuclear industries are implanted, are presented. the objective of these study was to provide useful data for the validation of models predicting the impact of these industries on the marine environment. even if differences were noted between sites for various metals, th ...201019452254
phylogenetic reconstruction using secondary structures and sequence motifs of its2 rdna of paragonimus westermani (kerbert, 1878) braun, 1899 (digenea: paragonimidae) and related species.most phylogenetic studies using current methods have focused on primary dna sequence information. however, rna secondary structures are particularly useful in systematics because they include characteristics that give "morphological" information, not found in the primary sequence. in several mountainous regions of northeastern india, foci of paragonimus (lung fluke) infection reportedly involve species that are known to prevail in neighbouring countries. the present study was undertaken to demon ...200919958489
juvenile colour polymorphism in the red rock crab, cancer productus: patterns, causes, and possible adaptive significance.juveniles of the common red rock crab of the northeastern pacific, cancer productus, display a stunning diversity of colours and patterns, while adults all have the same drab colouration. although this is widely known, key questions remain: (1) does the frequency of different juvenile colours or patterns vary among collection sites or seasonally? (2) does juvenile colour polymorphism reflect genetic heterogeneity or phenotypic plasticity in response to variable environmental conditions? (3) do j ...201020435453
bioaccessibility of hg, cd and as in cooked black scabbard fish and edible crab.regular consumption of seafood has been widely recommended by authorities. yet, some species accumulate high levels of contaminants like hg, cd and as. in addition, the risks associated to the consumption of such seafood may increase if consumers use cooking practices that enhance the concentration of contaminants and their bioaccessibility. in this study, the bioaccessibility of hg, cd and as was assessed with in vitro human digestion of raw and cooked black scabbard fish (hg; steamed, fried an ...201121856365
deoxyribonucleic acid of cancer pagurus. iv. elution behaviour on hydroxyapatite chromatographic column.fractionation of native dna on hydroxyapatite columns depends, when flat and continuous gradients are used, on the base composition, gc-rich fractions being eluted in the first fractions. crab satellite dna behaves abnormally : the first eluted fractions are enriched in poly d(a-t).d(a-t) instead of gc as usual. it amy be suggested that these differences in the behaviour could be attributed to the fact that the secondary structure of crab dna satellite is different from the secondary structure o ...20021241285
the pharmacological properties of some crustacean neuronal acetylcholine, gamma-aminobutyric acid, and l-glutamate responses.1. a study was performed of the l-glutamate, gamma-aminobutyric acid (gaba), and acetylcholine (ach) responses of cells in the stomatogastric ganglion of the crab, cancer pagurus. 2. ionophoretic or pressure application of l-glutamate revealed three classes of responses: a k+-dependent inhibition which reversed at 15-20 mv more negative than the resting potential; a cl- dependent inhibitory response which was at equilibrium at the resting potential; and a depolarizing response. 3. ionophoretic o ...1988211227
isolation & identification of bacteria for the treatment of brown crab (cancer pagurus) waste to produce chitinous material.to isolate bacteria from soil for microbial pretreatment of brown crab (cancer pagurus) shell waste and the production of chitin.201525644656
application of ultrasound in combination with heat and pressure for the inactivation of spore forming bacteria isolated from edible crab (cancer pagurus).this research was performed to characterize the resistance of three different bacterial spore species isolated from pasteurized edible crab (cancer pagurus) meat to heat treatments and to assess the potential of manosonication (ms) and manothermosonication (mts) as an alternative for their inactivation. the spore-forming bacteria used in this study were bacillus mycoides, bacillus weihenstephanensis and psychrobacillus psychrodurans. the thermal resistance of these three species was determined a ...201626874861
advances in our understanding of the global diversity and distribution of hematodinium spp. - significant pathogens of commercially exploited crustaceans.hematodinium species are parasitic dinoflagellates known to infect a growing number of marine crustacean genera from around the world, many of which support important commercial fisheries. affected hosts undergo dramatic pathological alterations to their organs, tissues and hemolymph. there are no known control measures for this disease. economically important wild fished hosts known to be susceptible to hematodinium spp. include tanner crabs chionoecetes bairdi and snow crabs chionoecetes opili ...201222433998
regional variation in fish predation intensity: a historical perspective in the gulf of maine.regional variation in the intensity of fish predation on tethered brittle stars and crabs was measured at 30-33 m depths in the rocky subtidal zone at seven sites representing coastal and offshore regions of the gulf of maine, usa. analysis of covariance comparing the slopes of brittle star survivorship curves followed by multiple comparisons tests revealed five groupings of sites, with significantly greater predation rates in the two offshore than in the three coastal groups. brittle stars teth ...199228313516
a novel approach to the measurement of surfactant parameters in arthropod digestive juices.in arthropods, the determination of two important parameters of digestive juices, i.e. the total surfactant concentration and the critical micelle concentration (cmc), is challenging due to small sample volumes and low surfactant concentrations. in this work, we report a successful implementation of potentiometric titrations using the surfactant ion-selective electrode (sise) and the pyrene fluorescence method (pfm) for the determination of the total surfactant concentration and cmc in the diges ...201626969560
attraction of predatorily naive postlarval lobsters to extracts of metabolites of common prey:mytilus edulis, mya arenaria, cancer irroratus, andasterias vulgaris.postlarval lobsters (4th-7th stage) exclusively fed frozen brine shrimp (artemia saline) were assayed for food-search response to extracts and metabolites from four common prey: soft clams (mya arenaria), blue mussels (mytilus edulis), rock crabs (cancer irroratus), and sea stars (asterias vulgaris). concentrations of soluble primary amines, protein, and ammonia in prey tissues and metabolites were determined. no significant responses were observed for any prey metabolites diluted to 1 and 10%, ...198724302143
susceptibility to infection and pathogenicity of white spot disease (wsd) in non-model crustacean host taxa from temperate regions.despite almost two decades since its discovery, white spot disease (wsd) caused by white spot syndrome virus (wssv) is still considered the most significant known pathogen impacting the sustainability and growth of the global penaeid shrimp farming industry. although most commonly associated with penaeid shrimp farmed in tropical regions, the virus is also able to infect, cause disease and kill a wide range of other decapod crustacean hosts from temperate regions, including lobsters, crabs, cray ...201222484233
comparative analysis of the proximate and elemental composition of the blue crab callinectes sapidus, the warty crab eriphia verrucosa, and the edible crab cancer pagurus.the proximate composition and element contents of claw muscle tissue of atlantic blue crabs (callinectes sapidus) were compared with the native warty crab (eriphia verrucosa) and the commercially edible crab (cancer pagurus). the scope of the analysis was to profile the chemical characteristics and nutritive value of the three crab species. elemental fingerprints showed significant inter-specific differences, whereas non-significant variations in the moisture and ash contents were observed. in t ...201627441254
(1)h nmr metabolomic profiling of the blue crab (callinectes sapidus) from the adriatic sea (se italy): a comparison with warty crab (eriphia verrucosa), and edible crab (cancer pagurus).the metabolomic profile of blue crab (callinectes sapidus) captured in the acquatina lagoon (se italy) was compared to an autochthonous (eriphia verrucosa) and to a commercial crab species (cancer pagurus). both lipid and aqueous extracts of raw claw muscle were analyzed by (1)h nmr spectroscopy and mva (multivariate data analysis). aqueous extracts were characterized by a higher inter-specific discriminating power compared to lipid fractions. specifically, higher levels of glutamate, alanine an ...201626593533
adaptive considerations of temperature dependence of neuromuscular function in two species of summer- and winter-caught crab (carcinus maenas and cancer pagurus).the aim of this study was to determine seasonal differences in the temperature dependence of neuromuscular parameters of the dactylopodite walking leg closer muscle in two species of freshly caught summer and winter decapod crabs. the relatively stenothermal cancer pagurus (cp) and eurythermal carcinus maenas (cm) muscle resting potential (rp) hyperpolarised significantly with increasing experimental temperature. the muscle rp in cm was seasonally dependent at acute temperatures above 20 °c wher ...201525994492
biogeographic variation in behavioral and morphological responses to predation risk.the expression of prey antipredator defenses is often related to ambient consumer pressure, and prey express greater defenses under intense consumer pressure. predation is generally greater at lower latitudes, and antipredator defenses often display a biogeographic pattern. predation pressure may also vary significantly between habitats within latitudes, making biogeographic patterns difficult to distinguish. furthermore, invasive predators may also influence the expression of prey defenses in e ...201323001623
hematodinium sp. and its bacteria-like endosymbiont in european brown shrimp (crangon crangon).parasitic dinoflagellates of the genus hematodinium are significant pathogens affecting the global decapod crustacean fishery. despite this, considerable knowledge gaps exist regarding the life history of the pathogen in vivo, and the role of free living life stages in transmission to naïve hosts.201222958655
physiological responses of three crustacean species to infection by the dinoflagellate-like protist hematodinium (alveolata: syndinea).this is the first study comparing physiological responses of three decapod species to infection by parasites of the genus hematodinium, which belongs to the dinoflagellate-like syndinea. responses varied profoundly between the crabs carcinus maenas and cancer pagurus (brachyura), but also differed to those of hermit crabs, pagurus bernhardus (anomura). osmoregulatory capacity was reduced significantly in hematodinium-infected c. maenas, haemolymph ph increased in parasitised c. pagurus and p. be ...201020600085
effect of temperature on the spoilage rate of whole, unprocessed crabs: carcinus maenas, necora puber and cancer pagurus.shelf life of whole, initially live, crabs depended primarily on the storage conditions and the time at which death occurred. large differences in the time that individual crab species survived particular storage conditions resulted in wide variations in shelf-life. bacterial spoilage of carcinus maenas, necora puber and cancer pagurus, measured using aerobic plate counts, indicated that on ice at 4 degrees c whole unprocessed crabs had a shelf life approximately 9-11 days, at 4 degrees c approx ...200717189768
neuroendocrine regulation of carbonic anhydrase expression in the gills of the euryhaline green crab, carcinus maenas.the relationship between branchial carbonic anhydrase (ca) activity, ca gene expression and salinity, and potential mechanisms of regulation, was investigated in the euryhaline green crab, carcinus maenas, acclimated to 33 ppt and transferred to 10 ppt, and the stenohaline rock crab, cancer irroratus, acclimated to 32 ppt and transferred to 18 ppt. ca activity in green crabs acclimated to high and low salinity was a function of ca mrna expression, with low salinity exposure resulting in an incre ...200616788899
characterization of cdna encoding molt-inhibiting hormone of the crab, cancer pagurus; expression of mih in non-x-organ tissues.synthesis of ecdysteroids (molting hormones) by crustacean y-organs is regulated by a neuropeptide, molt-inhibiting hormone (mih), produced in eyestalk neural ganglia. we report here the molecular cloning of a cdna encoding mih of the edible crab, cancer pagurus. full-length mih cdna was obtained by using reverse transcription-polymerase chain reaction (rt-pcr) with degenerate oligonucleotides based upon the amino acid sequence of mih, in conjunction with 5'- and 3'-race. full-length clones of m ...200111707332
heterothermal acclimation: an experimental paradigm for studying the control of thermal acclimation in crabs.a method for the study of the control of the attainment of thermal acclimation has been applied to the crabs, cancer pagurus and carcinus maenas. crabs were heterothermally acclimated by using an anterior-posterior partition between two compartments, one at 8 degrees c and the other at 22 degrees c. one compartment held a three-quarter section of the crab including the central nervous system (cns), eye stalks, and ipsilateral legs; the other held a quarter section including the contralateral leg ...199910339617
a comparative immunocytochemical study of the hyperglycaemic, moult-inhibiting and vitellogenesis-inhibiting neurohormone family in three species of decapod crustacea.eyestalks of the palinuran species jasus lalandii and panulirus homarus, and the brachyuran species carcinus maenas, were examined with antisera raised against purified crustacean hyperglycaemic hormone (chh) of the astacidean species homarus americanus and procambarus bouvieri, as well as the brachyuran species cancer pagurus. other antisera used in this investigation were raised against purified moult-inhibiting hormone (mih) of c. pagurus and vitellogenesis-inhibiting hormone (vih) of h. amer ...19999931363
"exolysosomes," enzyme-containing vesicles in the ecdysial space of molting crabs.free vesicle-like bodies (vlbs) present in the ecdysial space of cuticle regions undergoing degradation during preecdysis of the atlantic shore crab carcinus maenas have been interpreted either as infectious organisms or as secretion structures associated with degradation of the old cuticle. ultrastructural, cytochemical, and immunocytological investigations were performed to test these hypotheses and to see whether vlbs are peculiar to this crab species. similar vlbs were systematically found i ...19979245765
does the n-terminal pyroglutamate residue have any physiological significance for crab hyperglycemic neuropeptides?a characteristic feature of all crustacean hyperglycemic hormones (chh) is that they are always present in the sinus gland as multiple forms or isoforms. the amino acid sequence of the minor form of chh from the green shore crab, carcinus maenas, was determined by automated microsequencing and ms, and was almost identical to that of the major form, except that the n-terminal residue was glutamine rather than pyroglutamate. limited analysis (electrospray ms and amino acid composition) of the two ...19968841399
putative neurohemal release zones in the stomatogastric nervous system of decapod crustaceans.the stomatogastric nervous system (stns) of decapod crustaceans has long been used to study the modulation of small neural circuits. profiles in the sheath of the nerves and ganglia of the stns, which contain only dense-core vesicles, have been described in electron microscopical studies (friend [1976] cell tissue res. 175:369-380; kilman and marder [1997] soc neurosci abstr. 23:477; skiebe and ganeshina [2000] j comp neurol 420:373-397). these profiles resemble those found in neurohemal organs ...200212378588
synaptic neuropil in nerves of the crustacean stomatogastric nervous system: an immunocytochemical and electron microscopical study.patches of peptide-immunoreactive varicosities have been found in nerves of the stomatogastric nervous system (stns) of decapod crustaceans. in the present study, these patches were examined in detail in the stomatogastric nerve (stn) and in the superior oesophageal nerve (son) of the crayfish cherax destructor by using whole-mount immunocytochemical techniques combined with confocal microscopy and, in addition, electron microscopy. double-labeling experiments with antibodies generated against t ...200010754509
allatostatin-like immunoreactivity in the stomatogastric nervous system and the pericardial organs of the crab cancer pagurus, the lobster homarus americanus, and the crayfish cherax destructor and procambarus clarkii.the distribution of allatostatin (ast)-like immunoreactivity was studied in the stomatogastric nervous system (stns) and the neurosecretory pericardial organs (po) of four decapod crustacean species by using wholemount immunocytochemical techniques and confocal microscopy. ast-like immunoreactivity was found within the stns of all four species; its distribution in each was unique. in all four species, ast-like immunoreactivity was present in the paired commissural ganglia (cog), in the esophagea ...199910075445
molecular cloning of manganese superoxide dismutase gene in the cladoceran daphnia magna: effects of microcystin, nitrite, and cadmium on gene expression profiles.superoxide dismutases (sods) are metalloenzymes that represent one important line of defense against oxidative stress produced by reactive oxygen species in aerobic organisms. generally, waterborne pollutants caused by irregular anthropogenic activities often result in oxidative damage in aquatic organisms. the aim of this study was to molecularly characterize the manganese superoxide dismutase gene (dm-mnsod) in the waterflea, daphnia magna, and evaluate the mrna expression patterns quantified ...201424463312
single and multi-gene phylogeny of hepatospora (microsporidia) - a generalist pathogen of farmed and wild crustacean hosts.almost half of all known microsporidian taxa infect aquatic animals. of these, many cause disease in arthropods. hepatospora, a recently erected genus, infects epithelial cells of the hepatopancreas of wild and farmed decapod crustaceans. we isolated hepatospora spp. from three different crustacean hosts, inhabiting different habitats and niches; marine edible crab (cancer pagurus), estuarine and freshwater chinese mitten crab (eriocheir sinensis) and the marine mussel symbiont pea crab (pinnoth ...201627001103
effects of osmotic shock on the ultrastructure of cell nuclei in euryhaline and stenohaline crustaceans.changes in cell ultrastructure in relation to volume regulation following application of osmotic challenges have been but poorly documented up to now. in a previous study, we demonstrated that large and immediate changes in the organization of nuclear chromatin occur in mammalian cells under such conditions. similar chromatin modifications are shown in the present study in the cells of the stenohaline crab maia verrucosa. they are not seen in the cells of the euryhaline species tested, hyperosmo ...199118621193
determination of mercury in seafood by flow injection-cold vapor atomic absorption spectrometry after microwave digestion: nmkl interlaboratory study. nordic committee on food analysis.ten laboratories participated in an interlaboratory method-performance (collaborative) study of a method for the determination of mercury in foods of marine origin by flow injection-cold vapor atomic absorption spectrometry after wet digestion using a microwave oven technique. the study was preceded by a training round of samples of known identity. the method was tested on a total of 7 seafood products: blue mussel (mytilus edulis), cod muscle (gadus morhua), crab (cancer pagurus), scampi (nephr ...201312083254
disease profiles of juvenile edible crabs (cancer pagurus l.) differ at two geographically-close intertidal sites.the prevalence of disease in edible crabs (cancer pagurus) was assessed at two sites in south west wales; one estuarine (pembroke ferry) and another facing open water (freshwater east). diseases included pink crab disease caused by hematodinium sp., an infection of the antennal gland caused by paramikrocytos canceri and an idiopathic inflammatory condition of the connective tissue surrounding the anterior ganglionic masses. this latter condition was only found in crabs from pembroke ferry. there ...201525892036
are european lobsters (homarus gammarus) susceptible to infection by a temperate hematodinium sp.?hematodinium spp. infect over 40 species of crustaceans worldwide, but have not been reported to infect the european lobster, homarus gammarus. in this study, hematodinium parasites (a mixture of uni- and multinucleate trophont-like stages) were taken from donor crabs (cancer pagurus) and injected into juvenile h. gammarus. juvenile c. pagurus were also injected with the same inoculum. haemolymph was taken at regular intervals and examined for the presence of hematodinium using light microscopy ...201525721169
parasitization of juvenile edible crabs (cancer pagurus) by the dinoflagellate, hematodinium sp.: pathobiology, seasonality and its potential effects on commercial fisheries.this study reports on the prevalence and severity of infections caused by the parasitic dinoflagellate, hematodinium in juvenile edible crabs (cancer pagurus) found in 2 intertidal survey sites (mumbles head and oxwich bay) in the bristol channel, uk. crabs were assessed for the presence and severity of hematodinium infections by the histological examination of infected tissues. such infections were found to exhibit a seasonal trend in the 2 study areas with high numbers of animals (ca. 30%) inf ...201525118672
persistent organic pollutants in marine biota of são pedro and são paulo archipelago, brazil.remote islands, such as the são pedro and são paulo archipelago (spspa), brazil, are pristine areas. however, these locations are not exempt from the arrival of anthropogenic agents, such as persistent organic pollutants (pops). the present study aimed to determine the occurrence and distribution of pops in the marine biota of the spspa. sample extractions were performed using a microwave-assisted method. the predominant compounds were pcbs and ddts, which respectively had mean wet weight concen ...201323830520
paramarteilia canceri sp. n. (cercozoa) in the european edible crab (cancer pagurus) with a proposal for the revision of the order paramyxida chatton, 1911.a new species, paramarteilia canceri sp. n., is described using light and electron microscopy from the edible crab cancer pagurus l. captured from the english channel. no external symptoms were noted, although infected animals were typically lethargic and unresponsive to external stimuli. organs of infected animals were shrunken and collapsed compared with apparently healthy individuals. although the infection was systemic, marked host responses were only noted in the hepatopancreas where the pa ...200919606783
hematodinium sp. in irish cancer pagurus fisheries: infection intensity as a potential fisheries management tool.infection of cancer pagurus by a parasitic dinoflagellate of the genus hematodinium is described for the first time in ireland. an industry-based monitoring programme was established to determine seasonality of infection intensity and prevalence in the country's 3 largest brown crab fisheries in the southwest, north and southeast. the parasite was present in all areas for the majority of sampling periods, with highest prevalences recorded in pre-recruit animals of both sexes. microscopic examina ...200919301637
surface fine topography and pcr-based determination of metacercaria of paragonimus sp. from edible crabs in arunachal pradesh, northeast india.in several mountainous regions of northeastern india, foci of paragonimus infection reportedly involving species that are known to prevail in china have been identified. the present study was undertaken to demonstrate the surface fine topography and sequence analysis of the ribosomal deoxyribonucleic acid (rdna; second internal transcribed spacer, its2) of the metacercarial stages of the lung fluke collected from a mountain stream of the area (miao, changlang district in arunachal pradesh). the ...200717786478
enterospora sp., an intranuclear microsporidian infection of hermit crab eupagurus bernhardus.recent work at our laboratory has led to the discovery of a new genus of microsporidian parasite residing in the family enterocytozoonidae. the type species of this new genus, enterospora canceri, is an intranuclear parasite infecting the hepatopancreatocytes of the decapod crustacean cancer pagurus. here we provide the second description of a parasite within the genus enterospora, this time infecting the hermit crab eupagurus bernhardus from u.k. waters. the pathological manifestation and ultra ...200717523545
nectonema zealandica n. sp. (nematomorpha: nectonematoidea) parasitising the purple rock crab hemigrapsus edwardsi (brachyura: decapoda) in new zealand, with notes on the prevalence of infection and host defence reactions.a new species of marine hairworm, nectonema zealandica (nematomorpha: nectonematoidea), is described from the purple rock crab hemigrapsus edwardsi hilgendorf from the south island, new zealand. this is the first record of nectonema in the south pacific ocean and the southernmost locality for the genus. the description is based on juveniles and pre-adults taken from crabs. the new species is characterised by its stomal structure, presence of four cephalic papillae, mesenchyme arranged in eight l ...200111586084
experimental analysis of diet specialization in the snail kite: the role of behavioral conservatism.we examined factors maintaining extreme diet specialization in the snail kite (rostrhamus sociabilis), a medium-sized hawk which feed almost exclusively on pomacea snails, by determining why during some months kites eat crabs (dilocarcinus dentatus) in the ilanos of venezuela. we offered snails and crabs of different sizes to wild free-flying birds to develop estimates for a prey choice model. handling times of pomacea doliodes snails averaged 90±39 s and were positively correlated with snail si ...199428307027
characterization of three caspases and their pathogen-induced expression pattern in portunus trituberculatus.caspases are a family of proteases involved in many important biological processes including apoptosis and inflammation. in this study, we analyzed the expression patterns and effects on immune response in various tissues of the edible crab portunus trituberculatus. ptcas 2, ptcas 3 and ptcas 4 share overall sequence identities of 55.88%-74.86%, 8.47%-46.54% and 20.11%-50.87%, respectively, with their other crustacean species. ptcas 2, ptcas 3 and ptcas 4 have the same caspase domain and catalyt ...201728478258
lung function and prevalence of respiratory symptoms in norwegian crab processing workers.seafood processing workers have an increased risk of developing occupational asthma. this has not been studied among norwegian crab processing workers, nor has the respiratory health of exposed workers been compared to a control group.201728425826
Displaying items 1 - 95 of 95