| the first internal transcribed spacer (its-1) of ribosomal dna as a molecular marker for phylogenetic and population analyses in crustacea. | the objective of the present study is to explore the feasibility of using the first internal transcribed spacer (its-1) of ribosomal dna as a molecular marker for studying the interspecific and intraspecific genetic variations among crustaceans. we designed primers that could amplify its-1 from a majority of taxonomic groups of crustaceans. the gene was found to exhibit a high degree of length polymorphism among different groups, ranging from 182 bp in the barnacle balanus amphitrite to approxim ... | 2001 | 14961351 |
| partial amino acid sequence of a hemocyanin subunit from palinurus vulgaris. | 1. hemocyanin from the spiny lobster palinurus vulgaris was separated into two fractions, which were designated as subunits a and b. 2. 55% of the amino acid sequence of subunit b has been determined. a comparison with panulirus interruptus hemocyanins shows 78% sequence identity with subunit a and 56% with subunit c. it has carbohydrate attached to domain one. two half-cystines have been substituted, indicating that it probably possesses only one disulfide bridge. heterogeneity has been observe ... | 2001 | 2620502 |
| [the effects of flavoxate hydrochloride on the gastrointestinal motility (author's transl)]. | the experiments were performed to study the effects of flavoxate hydrochloride on the gastrointestinal motility and its underlying mechanism. gastrointestinal motility was inhibited at first and then accelerated markedly after the intravenous injection of flavoxate hydrochloride (10 mg/kg) in the experiments of anesthetized dogs. for analyzing the underlying mechanism of this response the following experiments were executed. 1) the pendular movements of isolated ileum of rabbits were accelerated ... | 1975 | 1172143 |
| crustin, a wap domain containing antimicrobial peptide from freshwater prawn macrobrachium rosenbergii: immune characterization. | crustin (mrcrs) was sequenced from a freshwater prawn macrobrachium rosenbergii. the mrcrs protein contains a signal peptide region at n-terminus between 1 and 22 and a long whey acidic protein domain (wap domain) at c-terminus between 57 and 110 along with a wap-type 'four-disulfide core' motif. phylogenetic results show that mrcrs is clustered together with other crustacean crustin groups. mrcrs showed high sequence similarity (77%) with crustin from pacific white shrimp litopenaeus vannamei a ... | 2013 | 23069787 |
| characterization of two isoforms of japanese spiny lobster panulirus japonicus defensin cdna. | antimicrobial peptides (amps) are components of the innate immune responses that form the first line of host defense against pathogens. in this study, cdnas of two new isoforms of defensin (designated pjd1 and pjd2) from a japanese spiny lobster panulirus japonicus haemocytes cdna library were cloned and sequenced. pjd1 and pjd2 consist of 656 and 673 nucleotides encoding putative proteins of 66 and 64 amino acids, respectively. the isoforms share 74.2% amino acid identity. in a phylogenetic ana ... | 2009 | 19073210 |
| investigation on natural diets of larval marine animals using peptide nucleic acid-directed polymerase chain reaction clamping. | the stomach contents of the larvae of marine animals are usually very small in quantity and amorphous, especially in invertebrates, making morphological methods of identification very difficult. nucleotide sequence analysis using polymerase chain reaction (pcr) is a likely approach, but the large quantity of larval (host) dna present may mask subtle signals from the prey genome. we have adopted peptide nucleic acid (pna)-directed pcr clamping to selectively inhibit amplification of host dna for ... | 2011 | 20535520 |