| predation of biomphalaria and non-target molluscs by the crayfish procambarus clarkii: implications for the biological control of schistosomiasis. | the north american crayfish procambarus clarkii was examined under laboratory conditions for its ability to prey on biomphalaria pfeifferi and b. glabrata, molluscan intermediate hosts of human schistosomiasis, and other, non-target gastropod species. both male and female adult crayfish significantly reduced survival of neonate snails, even though alternative animal and plant foods were both available. in subsequent experiments, no differences in snail consumption were detected, for either adult ... | 1992 | 1304709 |
| actions of three structurally distinct sea anemone toxins on crustacean and insect sodium channels. | the membrane actions of three recently isolated polypeptide neurotoxins from the sea anemones stichodactyla helianthus (toxin shi), condylactis gigantea (toxin cgii) and calliactis parasitica (toxin cpi) were investigated on action potentials and voltage-clamp membrane currents of the giant axon of the crayfish procambarus clarkii. the first two toxins were also tested on the cockroach (periplaneta americana) giant axon. all three toxins were particularly lethal to crustaceans, moderately toxic ... | 1992 | 1336629 |
| control of schistosome-transmitting snails in kenya by the north american crayfish procambarus clarkii. | snail-transmitted trematode parasites such as schistosomes and liver flukes assume considerable medical and veterinary significance in tropical africa. we have observed a strong negative association between the presence of medically important pulmonate snails and the crayfish procambarus clarkii in freshwater habitats in kenya. this crayfish, introduced into kenya around 1970, readily consumes these snails in the laboratory. field enclosure experiments indicate that crayfish exert a significant ... | 1991 | 1928568 |
| mapping of serotonin-like immunoreactivity in the ventral nerve cord of crayfish. | whole-mount immunohistochemical technique using antibody to serotonin (5-ht) had been used to map presumptive serotoninergic structures in the ventral abdominal and thoracic nerve cord of crayfish procambarus clarkii. 5-ht immunoreactivity was detected in more than 30 cell bodies, numerous fibers and peripheral nerve endings of root plexus and neuropilar regions. immunoreactive fibers are arranged in 3 pairs of rostrocaudal bundles. the median (mfb) and the lateral fiber bundles run longitudinal ... | 1990 | 2207659 |
| characterization of the microsomal mixed-function oxygenase system of the hepatopancreas and green gland of the red swamp crayfish, procambarus clarkii. | 1. the microsomal mixed-function oxygenase (mfo) system from the hepatopancreas and green gland of the red swamp crayfish, procambarus clarkii has been characterized with respect to the constitution of electron transport proteins and the ability to catalyze the metabolism of xenobiotics. 2. cytochrome p-450 content of hepatopancreas microsomes was approximately 10-fold higher than that of green gland and comparable to that of rat liver. nadph-cytochrome c reductase activity in hepatopancreas mic ... | 1989 | 2494018 |
| the arthropod gap junction and pseudo-gap junction: isolation and preliminary biochemical analysis. | the hepatopancreas of the crayfish, procambarus clarkii, contains an unusual abundance of gap junctions, suggesting that this tissue might provide an ideal source from which to isolate the arthropod-type of gap junction. a membrane fraction obtained by subcellular fractionation of this organ contained smooth septate junctions, zonulae adhaerentes, gap junctions and pentalaminar membrane structures (pseudo-gap junctions) as determined by electron microscopy. a further enrichment of plasma membran ... | 1988 | 2830976 |
| dose-mortality responses of crawfish and mosquitoes to selected pesticides. | a study was conducted to determine the toxicities (lc50s) of several pesticides on the commercially important red swamp crawfish, procambarus clarkii, and 3 mosquito species common in louisiana ricelands--anopheles quadrimaculatus, culex salinarius and psorophora columbiae. pesticides tested in laboratory bioassays included bacillus sphaericus, b. thuringiensis var. israelensis, bendiocarb, glyphosate, isostearyl alcohol, malathion, propoxur, resmethrin synergized with piperonyl butoxide (pbo) a ... | 1987 | 2904959 |
| phylogenetic study of the oxytocin-like immunoreactive system in invertebrates. | 1. a phylogenetic study of oxytocin (oxt)-like immunoreactive cells was performed by the pap method in the central nervous system of invertebrates. 2. the immunoreactivity was detected in the nerve cells of hydra magnipapillata of the coelenterata; neanthes japonica and pheretima communissima of the annelida; oncidium verrucosum, limax marginatus and meretrix lamarckii of the mollusca; and baratha brassica of the arthropoda. 3. no immunoreactive cells were found in bipalium sp. of the platyhelmi ... | 1988 | 2907439 |
| phylogenetic study of the arginine-vasotocin/arginine-vasopressin-like immunoreactive system in invertebrates. | 1. a phylogenetic study of arg-vasotocin (avt)/arg-vasopressin (avp)-like immunoreactive cells was performed by the pap method in the central nervous system of invertebrates. 2. the immunoreactivity was detected in the nerve cells of hydra magnipapillata of the coelenterata; neanthes japonica and pheretima communissima of the annelida; pomacea canaliculata, aplysia kurodai, oncidium verrucosum, bradybaena similaris, achatina fulica, limax marginatus and meretrix lamarckii of the mollusca; gnorim ... | 1988 | 2907440 |
| lead and cadmium concentrations in red crayfish (procambarus clarkii, g.) in the guadalquivir river marshes (spain). | | 1988 | 3355236 |
| potassium homeostasis in the nervous system of cephalopods and crustacea. | 1. previous work has shown that nerve activity is associated with a significant release of potassium in the vicinity of the axonal membrane. several mechanisms are normally present which reduce k+ accumulation in the extra-axonal space. 2. in intact connectives of the crayfish, procambarus clarkii, repetitive stimulation of the giant axons was associated with an apparent hyperpolarization measured by an interstitial microelectrode, which most probably corresponds to depolarization of the inner f ... | 1987 | 3503934 |
| oxygen uptake by excised gills of procambarus clarkii (girard) from albufera lake of valencia, spain, under heavy metal treatments. | | 1986 | 3719130 |
| toxicity of some ricefield pesticides to the crayfish p. clarkii, under laboratory and field conditions in lake albufera (spain). | static toxicities in mature crayfish (procambarus clarkii) were determined, for eight insecticides, two herbicides and one fungicide which were frequently used in rice cultivation at lake albufera in valencia (spain). three concentrations of each product were used: a) the concentration that was recommended by the dealer (in mg/l of active ingredient): carbofuran 0.40, carbaryl 0.80, malathion 0.80, trichlorfon 0.40, endosulfan 0.56, lindane 0.56, fenitrothion 0.40, cyfloxylate 0.02, molinate-thi ... | 1986 | 3819333 |
| single and multiple visual systems in arthropods. | extraction of two visual pigments from crayfish eyes prompted an electrophysiological examination of the role of visual pigments in the compound eyes of six arthropods. the intact animals were used; in crayfishes isolated eyestalks also. thresholds were measured in terms of the absolute or relative numbers of photons per flash at various wavelengths needed to evoke a constant amplitude of electroretinogram, usually 50 microv. two species of crayfish, as well as the green crab, possess blue- and ... | 1968 | 5641632 |
| sequence of the cdna encoding an actin homolog in the crayfish procambarus clarkii. | a cdna library was constructed by using mrnas purified from crayfish (procambarus clarkii) muscle. using a homology search of the nucleotide (nt) sequences, a clone of the library was found to encode a protein homologous to actin (act). the insert fragment of this cdna clone was 1072 nt in length. the amino acid sequence deduced from the nt sequence showed significant similarity to act of various organisms as follows: 88.1% to drosophila melanogaster, 88.2% to silk worm, 87.3% to brine shrimp, 8 ... | 1993 | 8224920 |
| light-induced binding of proteins to rhabdomeric membranes in the retina of crayfish (procambarus clarkii). | light-induced protein interaction as part of the process of visual transduction in arthropods with rhabdomeric photoreceptors was investigated biochemically by using crayfish retina. two kinds of retinal buffer soluble proteins (one of 40 kda and the other of 46 kda) were found to bind to the irradiated rhabdomeric membranes both in vitro and in vivo. the proteins bound to the membranes in the presence of metarhodopsin. an antibody against mouse arrestin (s-antigen) cross-reacted with the 40 kda ... | 1993 | 8249320 |
| trace elements in crayfish: regional differences and changes induced by cooking. | the concentrations of pb, cd, zn, cu, ni, cr, mn, co, as, and se were determined in the abdominal muscle and hepatopancreas from raw and cooked crayfish of astacus astacus (l.) and pacifastacus leniusculus (dana), both of swedish origin. after cooking, the concentrations of cd, ni, and co in the hepatopancreas showed a decrease and the concentrations of pb, cu, and mn in the abdominal muscle showed an increase. metal and metalloid concentrations were also determined in the abdominal muscle and h ... | 1994 | 8311505 |
| carbofuran poisoning in herons: diagnosis using cholinesterase reactivation techniques. | exposure to the carbamate insecticide carbofuran was detected using brain cholinesterase (che) reactivation techniques in heron carcasses collected from a potential pesticide exposure incident. great egrets (nycticorax nycticorax), great blue herons (ardea herodias), and black-crowned night herons (casmerodius albus) were exposed to carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate) either by dermal exposure while wading or through ingestion of contaminated food items. carcasse ... | 1995 | 8583636 |
| amino acid sequence of a peptide with molt-inhibiting activity from the kuruma prawn penaeus japonicus. | six major peptides (pej-sgp-i-vi) that belong to the chh family have been isolated from the sinus gland extracts of the kuruma prawn penaeus japonicus. by in vitro assay using the y-organ of the crayfish procambarus clarkii, pej-sgp-iv was found to be active in inhibiting ecdysteroid synthesis. we determined the complete amino acid sequence. pej-sgp-iv consists of 77 amino acid residues, with both free aimno- and carboxyl-termini. the sequence of pej-sgp-iv shows considerable similarity to that ... | 1996 | 8801521 |
| slow inhibition of na+ current in crayfish axons by 2-(1non-8enyl)-5-(1non-8enyl)pyrrolidine (pyr9), a synthetic derivative of an ant venom alkaloid | 2,5-dialkylpyrrolidines present in the venom of ants from the genus monomorium are natural insecticides causing a flaccid paralysis. the mechanism of action of 2-(1non-8enyl)-5-(1non-8enyl)pyrrolidine (pyr9), a synthetic derivative of 2,5-dialkylpyrrolidines, has been investigated in vitro on preparations of the ventral nerve cord of the crayfish procambarus clarkii. our results clearly indicate that pyr9 blocks spike conduction without affecting the resting potential. voltage-clamp experiments ... | 1997 | 9320008 |
| benzo[a]pyrene metabolism by the hepatopancreas and green gland of the red swamp crayfish, procambarus clarkii, in vitro. | the aim of this study was to determine the potential of activating the pro-carcinogen benzo[a]pyrene (b[a]p), elucidating b[a]p metabolite profiles, and to determine pyridine nucleotide-independent peroxygenase activity of procambarus clarkii hepatopancreas and green gland microsomal cytochromes p450 in vitro. we compare these data to metabolite profiles generated with the rat (rattus norvegicus) system. the major nad(p)h-dependent metabolite formed by both hepatopancreas and green gland microso ... | 1997 | 9467887 |
| microinjection of mrna encoding rat synapsin ia alters synaptic physiology in identified motoneurons of the crayfish, procambarus clarkii. | studies of identified neurons have made important contributions to our understanding of cellular neurophysiology. we have developed a technique for modifying gene expression in identified motoneurons of the crayfish procambarus clarkii in the isolated nervous system as well as in the intact animal through the injection of exogenously synthesized rnas. mrna suitable for injection was transcribed in vitro from cdna templates cloned into a plasmid, psem. initially, mrnas encoding green fluorescent ... | 1998 | 9805269 |
| safety and quality of fresh water crayfish procambarus clarkii in the river nile. | fresh water crayfish procambarus clarkii which recently appeared in river nile and its resources all over egypt was microbiologically and chemically evaluated in order to determine its safety and quality as human food. the average aerobic plate and enterobacteriaceae counts/g were 1.6 x 10(8) and 3.5 x 10(5), and the coliforms and e. coli were isolated from 100% and 40% of the examined samples respectively. however, salmonellae could not be isolated from any of the examined samples. 20% of the s ... | 1999 | 10331212 |
| predation of aquatic stages of anopheles gambiae by the louisiana red swamp crawfish (procambarus clarkii). | laboratory experiments lasting 1-3 days were conducted in 10-liter glass aquaria to evaluate the ability of the louisiana red swamp crawfish (procambarus clarkii [decapoda: cambaridae]) to consume the aquatic forms of mosquitoes. with anopheles gambiae as the target species, significantly fewer mosquito larvae or pupae survived in the presence of either juvenile or adult crawfish, relative to conditions without crawfish, regardless of whether crawfish had alternative food or not. when alternativ ... | 1999 | 10342272 |
| impact of the crayfish procambarus clarkii on schistosoma haematobium transmission in kenya. | the louisiana red swamp crayfish, procambarus clarkii, which was introduced into east africa in the 1950s or 1960s, has since widely dispersed. previous work by our group has shown that p. clarkii can reduce populations of the molluscan intermediate hosts of human schistosomes through predatory and competitive interactions. here, we investigate whether crayfish can reduce populations of bulinus africanus and consequently, schistosoma haematobium prevalence in school children. children from 6 pri ... | 1999 | 10586907 |
| food aversion learning induced by lithium chloride in the crayfish procambarus clarkii. | the ability of the crayfish procambarus clarkii to develop a food aversion learning was examined. for this purpose, in crayfishes fed with trout, a novel food (chicken) was associated in a single trial with a well-known toxic substance, lithium chloride (licl), and food intake was recorded for 10 days afterward using a two-choice protocol. all during the experimental period, a significant effect of treatment was observed that accounted for the lower chicken intake in the licl-treated group, comp ... | 2000 | 10764894 |
| cloning and characterization of sarco/endoplasmic reticulum ca(2+)-atpase (serca) from crayfish axial muscle. sarco/endoplasmic reticulum ca(2+)-atpase. | the discontinuous pattern of muscle growth during the moulting cycle of a freshwater crustacean (the crayfish procambarus clarkii) was used as a model system to examine the regulation of the expression of sarco/endoplasmic reticulum ca(2+)-atpase (serca). we describe the cloning, sequencing and characterization of a novel serca cdna (3856 bp) obtained from crayfish axial abdominal muscle by reverse transcription/polymerase chain reaction (rt-pcr) followed by rapid amplification of cdna ends (rac ... | 2000 | 11044380 |
| broad antiviral activity in tissues of crustaceans. | innate antiviral substances occur in vertebrates and may function as host defenses. virus infections are common among invertebrates, but little is known about the ability of invertebrates to control viral infections. pre-existing antiviral substances may be particularly important, since invertebrates lack the antiviral defense conferred by specific immunity. in our study, we found that tissue extracts of blue crab (callinectes sapidus), shrimp (penaeus setiferus), and crayfish (procambarus clark ... | 2000 | 11080539 |
| purification of vitellin from grass shrimp palaemonetes pugio, generation of monoclonal antibodies, and validation for the detection of lipovitellin in crustacea. | much effort has been put into developing vitellogenin antibodies against a wide variety of aquatic vertebrate species to study potential estrogen or anti-estrogen endocrine disrupters. little work has been done on endocrine disruption in aquatic invertebrates. although some antibodies have been produced against blue crab and penaeid shrimp lipovitellin, they have only poor cross-reactivity with the important estuarine grass shrimp, palaemonetes pugio. vitellin was purified from eggs, monoclonal ... | 2000 | 11083030 |
| red swamp crawfish (procambarus clarkii): an alternative experimental host in the study of white spot syndrome virus. | the pathogenicity of white spot syndrome virus (wssv) for the red swamp crawfish (procambarus clarkii) was investigated after infection by intramuscular (i.m.) injection and oral route. the cumulative mortality of crawfish injected i.m. with wssv reached 100% in 5 days. after oral feeding wssv-infected kuruma shrimp (penaeus japonicus) muscle tissues to the crawfish the cumulative mortality of this host reached 100% in 11 days. on reinfection trials, all the crawfish fed wssv-infected crawfish m ... | 2000 | 11332281 |
| impact of a toxic and a non-toxic strain of microcystis aeruginosa on the crayfish procambarus clarkii. | the occurrence of cyanobacteria in many water bodies where crayfish such as procambarus clarkii are abundant leads to the possibility of toxin accumulation and food chain transfer. this paper describes the accumulation and depuration of microcystins from a microcystin and a non-microcystin producing strain of microcystis aeruginosa, on the survivorship, growth and nutritional status of p. clarkii. crayfish larvae were resistant to cyanobacteria and their toxins, surviving cyanobacteria densities ... | 2001 | 11478953 |
| waterborne outbreak of tularemia associated with crayfish fishing. | in 1997, an outbreak of human tularemia associated with hare-hunting in central spain affected 585 patients. we describe the identification of francisella tularensis biovar palaearctica in a second outbreak of ulceroglandular tularemia associated with crayfish (procambarus clarkii) fishing in a contaminated freshwater stream distant from the hare-associated outbreak. the second outbreak occurred 1 year after the first. | 2001 | 11485678 |
| sequence analysis of the ribosomal internal transcribed spacer dna of the crayfish parasite psorospermium haeckeli. | two morphotypes of the crayfish parasite psorospermium haeckeli were isolated from 2 crayfish species of different geographical origin. the oval-shaped sporocysts were obtained from the epidermal and connective tissue beneath the carapace of the noble crayfish astacus astacus from sweden and finland. elongated spores were isolated from the abdominal muscle tissue of the red swamp crayfish procambarus clarkii from usa. to compare genetic divergence of 2 morphotypes of the parasite, the ribosomal ... | 2001 | 11710556 |
| the combined use of chemical and biochemical markers to assess water quality in two low-stream rivers (ne spain). | carps (cyprinus carpio) and red swamp crayfish (procambarus clarkii) were sampled from two lowstream mediterranean rivers (anoia and cardener) receiving extensive urban and industrial waste water discharges. tissue residues of selected pollutants (organochlorinated compounds, polycyclic aromatic hydrocarbons (pahs)) were determined in conjunction with different biochemical responses (cytochrome p450, phase ii enzymes) with the aim of investigating whether resident organisms were responsive to ch ... | 2002 | 12483808 |
| [tularemia possibly transmitted by red swamp crayfish]. | | 2003 | 12729545 |
| immunocytochemical and molecular data guide peptide identification by mass spectrometry: orcokinin and orcomyotropin-related peptides in the stomatogastric nervous system of several crustacean species. | in order to identify new orcokinin and orcomyotropin-related peptides in crustaceans, molecular and immunocytochemical data were combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). in the crayfish procambarus clarkii, four orcokinins and an orcomyotropin-related peptide are present on the precursor. because these peptides are highly conserved, we assumed that other species have an identical number of peptides. to identify the peptides, immun ... | 2003 | 14528921 |
| cloning and expression of a cdna encoding a matrix peptide associated with calcification in the exoskeleton of the crayfish. | calcification-associated peptide (cap)-1 isolated from the exoskeleton of the crayfish, procambarus clarkii, has anti-calcification activity and chitin-binding ability and is, therefore, considered to be associated with calcification. in this study, a cdna encoding cap-1 was cloned and characterized. an open reading frame encoded a pre-propeptide of 99 amino acid residues, which was composed of a signal peptide, a cap-1 precursor and two-basic amino acid residues at the c-terminus. the dibasic r ... | 2003 | 14662300 |
| identification of gyrkppfngsifamide (crustacean-sifamide) in the crayfish procambarus clarkii by topological mass spectrometry analysis. | a new concept relating to the purification protocol for biological proteins and peptides has been designed as "topological mass spectrometry analysis," in combination with maldi-tof ms using slices of tissues, chromatographic purification from the extract of tissues, molecular cloning for the determination of the precursor structure, and capillary lc-ms/ms analysis for elucidation of its posttranslational modifications. in an actual application, we identified an alpha-amidated neuropeptide from ... | 2004 | 14723891 |
| ecological risk assessment of neem-based pesticides. | a tiered process was used to evaluate the risks of pure azadirachtin (aza) and two neem-based insecticides (neemix and bioneem) on six aquatic animals [crayfish (procambarus clarkii), white shrimp (penaeus setiferus), grass shrimp (palaemonetes pugio), blue crabs (callinectes sapidus), water fleas (daphnia pulex), and mosquito larvae (culex quinquefasciatus)] through short term acute toxicity tests. the risk was calculated using the level of concern endpoints (q values) and relative hazard index ... | 2004 | 15132337 |
| molecular cloning of a putative membrane form guanylyl cyclase from the crayfish procambarus clarkii. | available data indicate that crustacean hyperglycemic hormone (chh) stimulates membrane-bound guanylyl cyclase (gc), producing cyclic guanosine 3',5'-monophosphate, which in turn mediates the effect of chh on carbohydrate metabolism. in the present study, we report the cloning of a cdna (pcgc-m2) encoding a putative membrane form gc from the muscle of the crayfish, procambarus clarkii. analysis of the deduced amino acid sequence shows that pcgc-m2 contains the signature domains characteristic of ... | 2004 | 15181645 |
| characterization and expression of plasma membrane ca2+ atpase (pmca3) in the crayfish procambarus clarkii antennal gland during molting. | the discontinuous pattern of crustacean cuticular mineralization (the molting cycle) has emerged as a model system to study the spatial and temporal regulation of genes that code for ca2+-transporting proteins including pumps, channels and exchangers. the plasma membrane ca2+-atpase (pmca) is potentially of significant interest due to its role in the active transport of ca2+ across the basolateral membrane, which is required for routine maintenance of intracellular ca2+ as well as unidirectional ... | 2004 | 15277554 |
| genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. | white spot syndrome virus (wssv) virions were purified from the hemolymph of experimentally infected crayfish procambarus clarkii, and their proteins were separated by 8 to 18% gradient sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) to give a protein profile. the visible bands were then excised from the gel, and following trypsin digestion of the reduced and alkylated wssv proteins in the bands, the peptide sequence of each fragment was determined by liquid chromatography-n ... | 2004 | 15452257 |
| orcokinins in insects and other invertebrates. | orcokinin (nfdeidrsgfgfn) and orcokinin homologues are crustacean peptides eliciting potent myotropic effects in gut tissues. through hplc purification of brain extract of the cockroach blattella germanica, we isolated the first insect orcokinin (nfdeidrsgfns). this insect orcokinin-like peptide do not show myotropic properties in b. germanica gut tissues. gene database search using orcokinin precursor sequences of the crustacean procambarus clarkii led to putative homologues found in non-crusta ... | 2004 | 15522610 |
| physiological and genetic characterisation of some new aphanomyces strains isolated from freshwater crayfish. | five aphanomyces strains were isolated during suspected outbreaks of crayfish disease in spain and italy. genetic and physiological evidence show that the strains isolated from the freshwater crayfish procambarus clarkii and pacifastacus leniusculus, do not fit into any previously identified group of aphanomyces astaci and are not capable of killing crayfish following standardised experimental infection. rapd-pcr and its sequencing analysis show a high degree of similarity between the new isolat ... | 2004 | 15530744 |
| response of crayfish, procambarus clarkii, haemocytes infected by white spot syndrome virus. | white spot syndrome virus (wssv) is a serious pathogen of aquatic crustaceans. little is known about its transmission in vivo and the immune reaction of its hosts. in this study, the circulating haemocytes of crayfish, procambarus clarkii, infected by wssv, and primary haemocyte cultures inoculated with wssv, were collected and observed by transmission electron microscopy and light microscopy following in situ hybridization. in ultra-thin sections of infected haemocytes, the enveloped virions we ... | 2005 | 15752275 |
| tissue distributions and seasonal dynamics of the hepatotoxic microcystins-lr and -rr in two freshwater shrimps, palaemon modestus and macrobrachium nipponensis, from a large shallow, eutrophic lake of the subtropical china. | so far no information is available on microcystin (mc) contents in shrimps, prawns or crayfish from natural freshwaters. tissue distributions and seasonal dynamics of the hepatotoxic mc-lr and -rr in two freshwater shrimps, palaemon modestus and macrobrachium nipponensis were studied monthly (during june-november, 2003) in a chinese lake containing toxic cyanobacterial blooms. the shrimps p. modestus and m. nipponensis accumulated high mcs not only in the hepatopancreas (mean 4.29 and 0.53 micro ... | 2005 | 15777958 |
| four differentially expressed cdnas in callinectes sapidus containing the rebers-riddiford consensus sequence. | decapod crustaceans such as callinectes sapidus, the blue crab, provide unique opportunities to study proteins involved in biomineralization. subsequent to each molt, the previously deposited soft cuticle is calcified while the postecdysial layers are simultaneously deposited and mineralized. though the majority of the exoskeleton hardens, morphologically similar cuticle at the joints, called arthrodial membrane, remains flexible. it seems reasonable that hypodermal cells producing these cuticle ... | 2005 | 15939643 |
| the almadén district (spain): anatomy of one of the world's largest hg-contaminated sites. | we present data from an early reconnaissance survey (stream sediments, soil, and water hg chemistry; plants and water crustaceans hg intake) of the almadén district (central spain), that was carried out to establish the potential environmental hazards derived from the anomalous mercury concentrations measured in this realm. the almadén mercury district (approximately 300 km2) can be regarded as the largest geochemical anomaly of mercury on earth. the district includes a series of mercury mineral ... | 2006 | 15950266 |
| a novel spiroplasma pathogen causing systemic infection in the crayfish procambarus clarkii (crustacea: decapod), in china. | a novel disease of crayfish procambarus clarkii appeared in the summer of 2004 in freshwater aquaculture in jiangsu province of china. light and transmission electron microscopy (tem), molecular biological methods and in vitro culture were used to identify the pathogen. the agent was unique in having a helical morphology and rotary motility as observed by phase-contrast light microscopy and was found in haemolymph, muscles, nerves and connective tissues by smear method and tem. ultra-thin sectio ... | 2005 | 16000238 |
| [effect of white spot syndrome virus envelope protein vp28 expressed in silkworm (bombyx mori) pupae on disease resistence in procambarus clarkii]. | the vaccine made of recombinant envelope protein (rvp28) of white spot syndrome virus (wssv) expressed in silkworm (bombyx mori) pupae using a baculovirus vector was used to investigate the efficacy of oral administration on wssv disease resistance of procambarus clarkii. vaccine was mixed with diet at a ratio of 2% (w/w), and procambarus clarkii were orally administered throughout 75 days. vaccination with rvp28 showed the significantly higher cumulative survival compared with positive and nega ... | 2005 | 16044911 |
| identification of an envelope protein (vp39) gene from shrimp white spot syndrome virus. | white spot syndrome virus (wssv) was purified from the tissues of experimentally infected crayfish (procambarus clarkii) with high yield. based on sds-page of purified wssv and mass spectrometry analysis, a protein with the molecular mass of 39 kda was identified to match an open reading frame (orf), wsv339, of wssv genome. this orf was 849 bp in length, encoding a 283 amino acid polypeptide. the protein was named vp39 and its gene was termed as vp39. temporal transcription analysis revealed tha ... | 2006 | 16132182 |
| transformation of organoarsenical species by the microflora of freshwater crayfish. | a study of the transformation of arsenic species by the microflora of the freshwater crayfish procambarus clarkii was carried out. the study of the degradation of ab (arsenobetaine) was performed in aerobic conditions in two culture media (tryptic soy broth and saline medium) at two temperatures (30 and 8 degrees c). the microflora transformed ab into tmao (trimethylarsine oxide), dma (dimethylarsinate), ma (methylarsonate), and an unidentified compound (u1). the quickest transformations were ca ... | 2005 | 16366730 |
| demonstration of nitric oxide synthase activity in crustacean hemocytes and anti-microbial activity of hemocyte-derived nitric oxide. | we determined the biochemical characteristics of nitric oxide synthase (nos) in hemocytes of the crayfish procambarus clarkii and investigated the roles of hemocyte-derived no in host defense. biochemical analysis indicated the presence of a ca2+ -independent nos activity, which was elevated by lipopolysaccharide (lps) treatment. when bacteria (staphylococcus aureus) and hemocytes were co-incubated, adhesion of bacteria to hemocytes was observed. no donor sodium nitroprusside (snp) significantly ... | 2006 | 16516518 |
| what is molt-inhibiting hormone? the role of an ecdysteroidogenesis inhibitor in the crustacean molting cycle. | the in vivo molt-inhibitory effects of the ecdysone biosynthesis inhibitors 3-hydroxy-l-kynurenine and xanthurenic acid were investigated. these ecdysone biosynthesis inhibitors, isolated from the eyestalks of blue crabs (callinectes sapidus), were injected into eyestalk-ablated crayfish (procambarus clarkii). the active factor was found to be species-nonspecific within crabs and crayfish. the seasonal profiles of the xanthurenic acid and ecdysone titers exhibited a staggered relationship. moreo ... | 1989 | 16594067 |
| the efficacy of recombinant vaccines against white spot syndrome virus in procambarus clarkii. | the effectiveness of oral, mock-, and immersion vaccination was investigated against white spot syndrome virus (wssv) in crayfish. the most exposed wssv envelope proteins vp19 and vp28 were used in different compositions and with different modes of applications. in experiment 1 crayfish were fed recombinant protein coated food pellets for 25 days, in experiment 2 the purified proteins were directly injected to them followed by one booster dose on 5th day and in experiment 3 the crayfish were lef ... | 2006 | 16600384 |
| effects of long-term exposure of the red swamp crawfish procambarus clarkii to a mixture of two herbicides, 2,4-dichlorophenoxyacetic acid and monosodium methanearsonate, and associated human health risks. | 2,4-dichlorophenoxyacetic acid and monosodium methanearsonate are often sold in commercial mixtures. bioconcentration studies have been performed for each of these herbicides individually, but little information exists concerning long-term exposure to a mixture of these herbicides. the following study examined the uptake of arsenic in crawfish after long-term exposure to this mixture, and the health risks associated with consumption of these crawfish. bioconcentration and depuration experiments ... | 2004 | 16696187 |
| proteomic analysis of the major envelope and nucleocapsid proteins of white spot syndrome virus. | white spot syndrome virus (wssv) virions were purified from the tissues of infected procambarus clarkii (crayfish) isolates. pure wssv preparations were subjected to triton x-100 treatment to separate into the envelope and nucleocapsid fractions, which were subsequently separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the major envelope and nucleocapsid proteins were identified by either matrix-assisted laser desorption ionization-time of flight mass spectrometry or de ... | 2006 | 16928742 |
| effect of hyperthermia on the replication of white spot syndrome virus (wssv) in procambarus clarkii. | the effect of hyperthermia on the development of white spot syndrome virus (wssv) in the crayfish procambarus clarkii was studied by competitive pcr. crayfish were exposed to different temperatures (24 +/- 1 and 32 +/- 1 degrees c) after wssv injection. no mortality was observed when crayfish were held at 32 +/- 1 degrees c, but mortality reached 100% when crayfish were transferred to 24 +/- 1 degrees c. competitive pcr showed that viral levels at 32 +/- 1 degrees c remained at 10(5) copies mg(- ... | 2006 | 16956066 |
| detection of aphanomyces astaci in north american crayfish by polymerase chain reaction. | we present a pcr based method to detect aphanomyces astaci in north american crayfish. primers were designed to specifically amplify parts of the internal transcribed spacer (its) regions and the 5.8 rrna gene of a. astaci. a single round and a semi-nested assay were tested for their sensitivity and specificity. specificity of the pcr assays was tested against several closely related aphanomyces species, other oomycetes and some non-a. astaci dna that might be found in or on crayfish. the single ... | 2006 | 17067073 |
| white spot syndrome virus (wssv) transmission from rotifer inoculum to crayfish. | to test the possibility that shrimp pond rotifer resting eggs and hatched rotifers could transmit white spot syndrome virus (wssv) to crayfish (procambarus clarkii), we injected crayfish with rotifer and resting egg inocula that were wssv-positive only by dot-blot analysis of pcr products. no crayfish became wssv-positive after challenge with the resting egg inoculum. however, 1/15 crayfish became wssv-positive after challenge with the rotifer inoculum. the results demonstrated that rotifers con ... | 2007 | 17069846 |
| protection of procambarus clarkii against white spot syndrome virus using recombinant oral vaccine expressed in pichia pastoris. | the potential for oral vaccination of crayfish against white spot syndrome virus was investigated. the envelope proteins vp19 and vp28 were expressed in yeast (pichia pastoris). the expressed proteins were used as oral vaccines in different forms viz., in whole culture form, whole culture sonicated form, whole culture centrifuged supernatant form, and cell residue form. the recombinant proteins were mixed with food pellets and fed to crayfish for 25 days. the vaccinated groups were divided into ... | 2007 | 17118674 |
| doñana national park survey using crayfish (procambarus clarkii) as bioindicator: esterase inhibition and pollutant levels. | utility of carboxylesterase and acetylcholinesterase inhibition as pesticide exposure biomarker was studied at doñana national park (sw spain) in crayfish (procambarus clarkii). activities were measured in animals from reference sites or potentially exposed to pesticides, and their reactivation studied after dilution or 2-pam treatment. crayfish from affected sites had significantly less carboxylesterase and acetylcholinesterase activity than reference ones. no significant differences were found ... | 2007 | 17157454 |
| significance of the n- and c-terminal regions of cap-1, a cuticle calcification-associated peptide from the exoskeleton of the crayfish, for calcification. | calcification-associated peptide (cap)-1 is considered to play an important role in calcification of the exoskeleton of the crayfish, procambarus clarkii. in this study, in order to clarify the molecular mechanism of calcification, we constructed expression systems of recombinant molecules of cap-1 and its related peptides in escherichia coli, and verified the structure-activity relationship of these peptides. the inhibitory assay on calcium carbonate precipitation and the calcium-binding assay ... | 2007 | 17239990 |
| [influence of white spot syndrome virus envelope protein vp28 expressed in silkworm (bombyx mori) pupae on immune response in procambarus clarkii]. | silkworm (bombyx mori) pupae infected with recombinant baculovirus hynpv-vp28 were made vaccine,which was mixed with feed at a ratio of 2% (w/w). procambarus clarkii were orally administered. the results of immune analysis showed that the phagocytic percent and phagocytic index of hemocytes increased significantly when compared the rvp28 treatment with control treatments (p < 0.05). compared with control groups,the antibiotic activity, bacteriolytic activity and activities of phenoloxidase, supe ... | 2006 | 17348205 |
| toxicity of fipronil and its enantiomers to marine and freshwater non-targets. | fipronil is a phenylpyrazole insecticide used in agricultural and domestic settings for controlling various insect pests in crops, lawns, and residential structures. fipronil is chiral; however, it is released into the environment as a racemic mixture of two enantiomers. in this study, the acute toxicity of the (s,+) and (r,-) enantiomers and the racemic mixture of fipronil were assessed using simulium vittatum is-7 (black fly), xenopus laevis (african clawed frog), procambarus clarkii (crayfish ... | 2007 | 17562454 |
| conservation of structure, signaling and pharmacology between two serotonin receptor subtypes from decapod crustaceans, panulirus interruptus and procambarus clarkii. | serotonin (5-ht) plays important roles in the maintenance and modulation of neural systems throughout the animal kingdom. the actions of 5-ht have been well characterized for several crustacean model circuits; however, a dissection of the serotonergic transduction cascades operating in these models has been hampered by the lack of pharmacological tools for invertebrate receptors. here we provide pharmacological profiles for two 5-ht receptors from the swamp crayfish, procambarus clarkii: 5-ht(2b ... | 2008 | 18083737 |
| sifamide peptides in clawed lobsters and freshwater crayfish (crustacea, decapoda, astacidea): a combined molecular, mass spectrometric and electrophysiological investigation. | recently, we identified the peptide vyrkppfngsifamide (val(1)-sifamide) in the stomatogastric nervous system (stns) of the american lobster homarus americanus using matrix-assisted laser desorption/ionization-fourier transform mass spectrometry (maldi-ftms). given that h. americanus is the only species thus far shown to possess this peptide, and that a second sifamide isoform, gly(1)-sifamide, is broadly conserved in other decapods, including another astacidean, the crayfish procambarus clarkii, ... | 2008 | 18308319 |
| oral vaccination with envelope protein vp28 against white spot syndrome virus in procambarus clarkii using bacillus subtilis as delivery vehicles. | to achieve high-level expression and secretion of active vp28 directed by a processing-efficient signal peptide in bacillus subtilis wb600 and exploit the possibility of obtaining an oral vaccine against white spot syndrome virus (wssv) using vegetative cells or spores as delivery vehicles. | 2008 | 18373657 |
| phylogenetic analysis of spiroplasmas from three freshwater crustaceans (eriocheir sinensis, procambarus clarkia and penaeus vannamei) in china. | disease epizootics in freshwater culture crustaceans (crab, crayfish and shrimp) gained high attention recently in china, due to intensive developments of freshwater aquacultures. spiroplasma was identified as a lethal pathogen of the above three freshwater crustaceans in previous studies. further characterization of these freshwater crustacean spiroplasma strains were analyzed in the current study. phylogenetic position was investigated by analysis of partial nucleotide sequences of 16s ribosom ... | 2008 | 18621053 |
| lipid of white-spot syndrome virus originating from host-cell nuclei. | the hypothesis that white-spot syndrome virus (wssv) generates its envelope in the nucleoplasm is based on electron microscopy observations; however, as yet there is no direct evidence for this. in the present study, the lipids of wssv and the nuclei of its host, the crayfish procambarus clarkii, were extracted and the neutral lipid and phospholipid contents were analysed by high-performance liquid chromatography, thin-layer chromatography and gas chromatography/mass spectrometry. phosphatidylch ... | 2008 | 18931090 |
| crustacean molt-inhibiting hormone: structure, function, and cellular mode of action. | in crustacea, secretion of ecdysteroid molting hormones by y-organs is regulated, at least in part, by molt-inhibiting hormone (mih), a polypeptide neurohormone produced by neurosecretory cells of the eyestalks. this article reviews current knowledge of mih, with particular emphasis on recent findings regarding the (a) structure of the mih peptide and gene, (b) levels of mih in eyestalks and hemolymph, (c) cellular mechanism of action of mih, and (d) responsiveness of y-organs to mih. at least 2 ... | 2009 | 19000932 |
| identification of differentially expressed genes in haemocytes of the crayfish (procambarus clarkii) infected with white spot syndrome virus by suppression subtractive hybridization and cdna microarrays. | by using suppression subtractive hybridization (ssh) and cdna microarrays, we studied the differentially expressed genes in haemocytes of the crayfish (procambarus clarkii) infected with white spot syndrome virus (wssv). thirty three differentially expressed genes were detected in which 31 were up-regulated and 2 were down-regulated. the up-regulated genes include serine protease inhibitors, chaperonin, synaptasome-associated protein of 25 kd(snap25), tubulin, zinc-finger protein, intracellular ... | 2009 | 19071220 |
| protection of procambarus clarkii against white spot syndrome virus using inactivated wssv. | white spot syndrome virus (wssv) is a highly pathogenic and prevalent virus infecting shrimp and other crustaceans. the potentiality of binary ethylenimine (bei)-inactivated wssv against wssv in crayfish, procambarus clarkii, was investigated in this study. efficacy of bei-inactivated wssv was tested by vaccination trials followed by challenge of crayfish with wssv. the crayfish injected with bei-inactivated wssv showed a better survival (p<0.05) to wssv on the 7th and 21st day post-vaccination ... | 2009 | 19268545 |
| the use of monoclonal antibodies anti-lectin from freshwater prawn macrobrachium rosenbergii (deman, 1879) in the recognition of protein with lectin activity in decapod's hemolymph. | we determined the cross-reactivity of a monoclonal antibody against the macrobrachium rosenbergii lectin with proteins in the hemolymph from procambarus clarkii (pc), procambarus americanus (pa), litopenaeus setiferus (ls), and pseudothelphusa americana (psa). crustaceans' hemolymph agglutinated erythrocytes from rat, mouse, guinea pig, and rabbit. decapods' hemolymph hemagglutinating activity was inhibited by n-acetylated carbohydrates as well as by antibodies. western blot assays indicated tha ... | 2009 | 19431046 |
| primary diagnosis and surveillance of white spot syndrome virus in wild and farmed crawfish (procambarus clarkii, p. zonangulus) in louisiana, usa. | this is the first report of natural white spot syndrome virus (wssv) infection in wild and large-scale farmed crawfish. in the spring of 2007, 3 crawfish farms experienced heavy mortality in ponds populated by procambarus clarkii and p. zonangulus. histological examination revealed findings consistent with severe viral infection characterized by numerous intranuclear inclusions in ectodermal and mesodermal tissues. samples tested by in situ hybridization, injection bioassay in litopenaeus vannam ... | 2009 | 19593929 |
| a three-domain kazal-type serine proteinase inhibitor exhibiting domain inhibitory and bacteriostatic activities from freshwater crayfish procambarus clarkii. | in crustaceans, kazal-type serine proteinase inhibitors in hemolymph are believed to function as regulators of the host-defense reactions or inhibitors against proteinases from microorganisms. in this study, we report a kazal-type serine proteinase inhibitor, named hcpcspi1, from freshwater crayfish (procambarus clarkii). we found that hcpcspi1 is composed of a putative signal peptide, an rgd motif, and three tandem kazal-type domains with the domain p1 residues l, l and e, respectively. mainly, ... | 2009 | 19616577 |
| identification and molecular characterization of a spätzle-like protein from chinese shrimp (fenneropenaeus chinensis). | in invertebrates, the toll signaling pathway is important for activation of antimicrobial peptides in the innate immune system. activation of the toll pathway requires binding of toll with its ligand spätzle. here we described a spätzle-like protein, designated as fc-spz, from hemocytes of chinese shrimp, fenneropenaeus chinensis. the deduced amino acid sequence of fc-spz shares 54% identity with spätzle-like protein of salmon louse (lepeophtheirus salmonis). semi-quantitative reverse transcript ... | 2009 | 19616633 |
| comparative acute toxicity of neonicotinoid and pyrethroid insecticides to non-target crayfish (procambarus clarkii) associated with rice-crayfish crop rotations. | most insecticides used to control rice water weevil (lissorhoptrus oryzophilus kuscel) infestations are pyrethroids. however, pyrethroids are highly toxic to non-target crayfish associated with rice-crayfish crop rotations. one solution to the near-exclusive reliance on pyrethroids in a rice-crayfish pest management program is to incorporate neonicotinoid insecticides, which are insect specific and effective against weevils but not extremely toxic to crayfish. this study aimed to take the first ... | 2009 | 19623546 |
| a single wap domain (swd)-containing protein with antipathogenic relevance in red swamp crayfish, procambarus clarkii. | single wap domain-containing proteins (swds) are small proteins possessing a whey acidic protein (wap) domain at the c-terminal region. in this study, a complementary deoxyribonucleic acid (cdna) of swd-containing protein was isolated from the hemocytes of red swamp crayfish, procambarus clarkii called pc-swd. the full-length cdna sequence is 998 bp. the deduced amino acid sequence consists of 74 residues with a signal peptide of 20 residues. the mature peptide has a single wap domain which cont ... | 2010 | 19840856 |
| contaminant accumulation and multi-biomarker responses in field collected zebra mussels (dreissena polymorpha) and crayfish (procambarus clarkii), to evaluate toxicological effects of industrial hazardous dumps in the ebro river (ne spain). | large amounts of industrial waste containing high concentrations of mercury, cadmium and organochlorine residues were dumped in a reservoir adjacent to a chlorine-alkali plant in the village of flix(catalonia, spain), situated at the shore of the lower ebro river. effects of these contaminants to aquatic river invertebrates were assessed by integrating analyses of metals and organochlorine residues in field collected zebra mussels and crayfish with a wide range of biomarkers. biological response ... | 2010 | 19954812 |
| three kazal-type serine proteinase inhibitors from the red swamp crayfish procambarus clarkii and the characterization, function analysis of hcpcspi2. | three kazal-type serine proteinase inhibitors, hcpcspi2, hppcspi3, and hppcspi4, with complete cdna sequences, were identified from a cdna library of the red swamp crayfish, procambarus clarkii. semi-quantitative rt-pcr shows that hcpcspi2 exists mainly in hemocytes while both hppcspi3 and hppcspi4 were detected in the hepatopancreas and the heart. homology comparison and phylogenic analysis indicate that hppcspi3 and hppcspi4 shared high identity and formed the same group, and both of them were ... | 2010 | 20170735 |
| high prevalence of multiple paternity in the invasive crayfish species, procambarus clarkii. | reproductive strategy is a central feature of the ecology of invasive species as it determines the potential for population increase and range expansion. the red swamp crayfish, procambarus clarkii, has invaded many countries and caused serious problems in freshwater ecosystems. however, little is known about the effects of environmental conditions on crayfish paternity and offspring traits in the wild. we studied these reproductive characteristics of p. clarkii in wild populations from two diff ... | 2010 | 20186292 |
| nuclear dna content variation associated with muscle fiber hypertrophic growth in decapod crustaceans. | we tested the hypothesis that hypertrophic muscle growth in decapod crustaceans is associated with increases in both the number of nuclei per fiber and nuclear dna content. the dna-localizing fluorochrome dapi (4',6-diamidino-2-phenylindole) and chicken erythrocyte standards were used with static microspectrophotometry and image analysis to estimate nuclear dna content in hemocytes and muscle fibers from eight decapod crustacean species: farfantepenaeus aztecus, palaemonetes pugio, panulirus arg ... | 2010 | 20237594 |
| identification and characterization of alix/aip1 interacting proteins from the black tiger shrimp, penaeus monodon. | apoptosis is proposed to be a major cause of death in shrimp viral infections. from our previous study, an apoptosis-related gene, pm-alix, was identified from the black tiger shrimp. its expression was high in defence-related tissues including haemocytes and the lymphoid organ. to clarify its possible role in shrimp, we used pm-alix as bait in a yeast two-hybrid analysis to search for alix interacting proteins in shrimp. two cdna sequences discovered had homology to a predicted ubiquitin c of t ... | 2010 | 20412359 |
| functional analysis of two invertebrate-type lysozymes from red swamp crayfish, procambarus clarkii. | lysozyme is an important immune effector and is widely distributed in many organisms. in the present study, two novel invertebrate-type lysozymes (pclysi1 and pclysi2) were cloned from red swamp crayfish, procambarus clarkii. alignment analysis showed that these two genes were different in catalytic residues. results of rt-pcr showed that these two genes share similar tissue distribution patterns, and both were upregulated after bacteria challenge. the mature recombinant proteins were expressed ... | 2010 | 20816803 |
| a rapid assay for simultaneous detection of spiroplasma eriocheiris and white spot syndrome virus in procambarus clarkii by multiplex pcr. | to establish a multiplex pcr method for simultaneous and rapid detection of spiroplasma eriocheiris and white spot syndrome virus (wssv) in procambarus clarkii with recommendations for application to other crustacea. | 2010 | 20831654 |
| an anti-lipopolysaccharide factor from red swamp crayfish, procambarus clarkii, exhibited antimicrobial activities in vitro and in vivo. | the anti-lipopolysaccharide factors (alfs) are a group of effector molecules of innate immunity in arthropods, exhibiting binding and neutralizing activities to lipopolysaccharides. in this study, an alf cdna sequence (pcalf1) was identified from red swamp crayfish, procambarus clarkii. the deduced peptide of pcalf1 was conserved; it manifested the signal peptide and lipopolysaccharide (lps)-binding domain, especially the two conserved cysteine residues at both ends of the domain. transcripts of ... | 2010 | 21056107 |
| c-type lectin from red swamp crayfish procambarus clarkii participates in cellular immune response. | lectins are potential immune recognition proteins. in this study, a novel c-type lectin (pc-lec1) is reported in freshwater crayfish procambarus clarkii. pc-lec1 encodes a protein of 163 amino acids with a putative signal peptide and a single carbohydrate recognition domain. it was constitutively expressed in various tissues of a normal crayfish, especially in the hepatopancreas and gills. expressions of pc-lec1 were up-regulated in the hepatopancreas and gills of crayfish challenged with vibrio ... | 2011 | 21322006 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| permanent genetic resources added to molecular ecology resources database 1 december 2010-31 january 2011. | this article documents the addition of 238 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: alytes dickhilleni, arapaima gigas, austropotamobius italicus, blumeria graminis f. sp. tritici, cobitis lutheri, dendroctonus ponderosae, glossina morsitans morsitans, haplophilus subterraneus, kirengeshoma palmata, lysimachia japonica, macrolophus pygmaeus, microtus cabrerae, mytilus galloprovincialis, pallisentis (neosentis) celatus, ... | 2011 | 21457476 |
| increasing potential risk of a global aquatic invader in europe in contrast to other continents under future climate change. | anthropogenically-induced climate change can alter the current climatic habitat of non-native species and can have complex effects on potentially invasive species. predictions of the potential distributions of invasive species under climate change will provide critical information for future conservation and management strategies. aquatic ecosystems are particularly vulnerable to invasive species and climate change, but the effect of climate change on invasive species distributions has been rath ... | 2011 | 21479188 |
| involvement of peroxinectin in the defence of red swamp crayfish procambarus clarkii against pathogenic aeromonas hydrophila. | cell adhesion factors are important immune components for invertebrate to immobilize, phagocytose or encapsulate invasive microorganisms and foreign particles. in this study, a new cell adhesion factor, peroxinectin (refered as pcpxin) was isolated from hemocytes of red swamp crayfish (procambarus clarkii). the full-length cdna of pcpxin was 3014 bp encoding a protein of 819 amino acid residues with a predicted molecular weight of 89.0 kda and a calculational isoelectric point of 6.93. the putat ... | 2011 | 21539922 |
| effects of uranium uptake on transcriptional responses, histological structures and survival rate of the crayfish procambarus clarkii. | this work aims to investigate the accumulation levels and effects (transcriptional responses, histopathology and survival rate) associated with a wide range of dissolved uranium (u) concentrations (0, 0.03, 0.6, 4 and 8mg/l of u) on adult male crayfish procambarus clarkii during 4 (t4) and 10 (t10) days of exposure. the follow-up of the crayfish mortality showed that p. clarkii was highly resistant to u. increasing waterborne u concentrations led to increasing bioaccumulation in key crayfish org ... | 2011 | 21741087 |
| a putative g protein-coupled receptor involved in innate immune defense of procambarus clarkii against bacterial infection. | the immune functions of g protein-coupled receptor (gpcr) were widely investigated in mammals. however, limited researches on immune function of gpcrs were reported in invertebrates. in the present study, the immune functions of hp1r gene, a putative gpcr identified from red swamp crayfish procambarus clarkii were reported. expression of hp1r gene was significant up-regulated in response to heat-killed aeromonas hydrophila challenge. hp1r gene silencing mediated by rna interference significantly ... | 2011 | 21964155 |
| chemical defense of the eastern newt (notophthalmus viridescens): variation in efficiency against different consumers and in different habitats. | amphibian secondary metabolites are well known chemically, but their ecological functions are poorly understood--even for well-studied species. for example, the eastern newt (notophthalmus viridescens) is a well known secretor of tetrodotoxin (ttx), with this compound hypothesized to facilitate this salamander's coexistence with a variety of aquatic consumers across the eastern united states. however, this assumption of chemical defense is primarily based on observational data with low replicati ... | 2011 | 22164212 |
| analysis of variable genomic loci in white spot syndrome virus to predict its origins in procambarus clarkii crayfish farmed in china. | variable genomic loci were examined in 4 white spot syndrome virus (wssv) isolates (08hb, 09hb, 08js and 09js) from procambarus clarkii crayfish collected from jiangsu and hubei provinces in china in 2008 and 2009. in orf75, sequence variation detected in the 4 isolates, as well as in isolates sequenced previously, suggested that wssv might have segregated into 2 lineages since first emerging as a serious pathogen of farmed shrimp in east asia in the early-mid 1990s, with one lineage remaining i ... | 2011 | 22013750 |
| Effects of uranium on crayfish Procambarus clarkii mitochondria and antioxidants responses after chronic exposure: What have we learned? | We examined the impacts of Uranium (U) on mitochondria and on the response of antioxidants in the gills and the hepatopancreas of crayfish Procambarus clarkii after long-term exposure (30 and 60 days) to an environmentally relevant concentration (30µgU/L). The expression of mitochondrial genes (12s, atp6, and cox1), as well as the genes involved in oxidative stress responses (sod(Mn) and mt) were evaluated. The activities of antioxidant enzymes (SOD, CAT, GPX and GST) were also studied. U accumu ... | 2011 | 22154145 |
| A vector that expresses VP28 of WSSV can protect red swamp crayfish from white spot disease. | White spot disease caused by white spot syndrome virus (WSSV) leads to devastating losses in shrimp farming. The WSSV envelope protein VP28, can be used as subunit vaccines that can efficiently protect shrimp against WSSV disease. However, the function of the envelope protein VP19 was not confirmed, some researches found that VP19 could protect shrimp against WSSV, and other reports found it no any protection. To detect the functions of VP28 and VP19 and find a method to prevent this disease in ... | 2012 | 21906621 |
| recovery of salmonella from biofilms in a headwater spring ecosystem. | salmonellae are pathogenic bacteria often detected in waters impacted by human or animal wastes. in order to assess the fate of salmonellae in supposedly pristine environments, water and natural biofilm samples along with snails (tarebia granifera) and crayfish (procambarus clarkia) were collected before and up to 7 days following four precipitation events from sites within the headwater springs of spring lake, san marcos, tx. the samples were analyzed for the presence of salmonellae by polymera ... | 2011 | 21976193 |
| histological alterations and immune response in the crayfish procambarus clarkii given rvp28-incorporated diets. | there is growing evidence that recombinant vp28 protein (rvp28) can significantly enhance immune response and disease resistance against white spot syndrome virus (wssv) in shrimp, although the underlying mechanisms have not been entirely clarified yet. the aim of this study was to determine the effect of rvp28 on histological alterations and wssv-induced apoptosis in crayfish procambarus clarkii. crayfish were fed commercial diets supplemented with different doses of hynpv-vp28 infected pupae ( ... | 2011 | 22008287 |