| alpha2-macroglobulin from hemolymph of the freshwater crayfish astacus astacus. | 1. a high mol. wt proteinase inhibitor has been purified from the haemolymph of the freshwater crayfish astacus astacus. 2. the protein is a disulphide-bonded dimer (mr 390,000) of two identical polypeptide chains (mr 185,000). 3. the inhibitor displays a broad specificity and protects trypsin from inhibition by soybean trypsin inhibitor and thus is similar to vertebrate alpha 2-macroglobulin. 4. the alpha 2-macroglobulin-like inhibitor from astacus interacts with bovine trypsin in an equimolar ... | 1991 | 1714366 |
| purification and characterization of a beta-1,3-glucan binding protein from plasma of the crayfish pacifastacus leniusculus. | the plasma of the crayfish pacifastacus leniusculus contains a protein which is able to bind to laminarin (a soluble beta-1,3-glucan) and which has been isolated by two independent methods, affinity precipitation with a beta-1,3-glucan or immunoaffinity chromatography. the purified beta-1,3-glucan binding protein was homogenous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. it is a monomeric glycoprotein with a molecular mass of approximately 100,000 da and an isoelectri ... | 1990 | 2111817 |
| purification and characterization of an alpha 2-macroglobulin-like proteinase inhibitor from plasma of the crayfish pacifastacus leniusculus. | an alpha 2-macroglobulin (alpha 2m)-like proteinase inhibitor from plasma of the crayfish pacifastacus leniusculus was purified to apparent homogeneity by acid precipitation, hydrophobic interaction chromatography, affinity chromatography on concanavalin a-sepharose and anion-exchange chromatography. the subunit mr is about 190,000. pore-size-limit electrophoresis proved the native protein to be a dimer. the purified protein resembled vertebrate alpha 2 ms in that it protected trypsin from inhib ... | 1988 | 2463832 |
| amino acid sequence around the thiolester of alpha 2-macroglobulin from plasma of the crayfish, pacifastacus leniusculus. | alpha 2-macroglobulin (alpha 2 m) was isolated from plasma of the freshwater crayfish, pacifastacus leniusculus, using ultracentrifugation, ion-exchange chromatography and gel filtration techniques. the pacifastacus alpha 2 m molecule (p alpha 2 m) was radio-actively labeled in the thiol ester structure with iodo [14c]acetic acid in the presence of methylamine. after reduction and carboxymethylation of the protein, it was digested with trypsin. a 14c-labeled tryptic peptide was sequenced and con ... | 1989 | 2476331 |
| evolutionary studies on pancreatic colipase. | in this evolutionary study the following criteria have been used to prove the existence of colipase: 1. it restores the activity of human and porcine pancreatic lipase inhibited by bile salt. 2. it cross-reacts with antisera to human and porcine colipases. 3. its restoration of lipase activity, inhibited by bile salt, in the tributyrin assay system, is prevented by antiserum to colipase. 4. it is a heat-stable, low-molecular-weight protein (molecular weight about 10 000 by gel-filtration). the o ... | 1983 | 6860687 |
| assay of neuronal nitric oxide synthase by hplc determination of citrulline. | biological membranes from different tissue sources were incubated for nitric oxide synthase (nos) activity under standard conditions at 20 degrees c and compared with rat cerebellar cytosol. nos activity was monitored as the formation of l-citrulline from l-arginine. samples were purified on amprep cba cation-exchange minicolumns prior to derivatization with o-phthaldialdehyde (opa) and hplc analysis. the opa derivatives of l-citrulline and l-arginine eluted well separated within 15 min during i ... | 1994 | 7526085 |
| structure and biological activity of a 1,3-beta-d-glucan-binding protein in crustacean blood. | the prophenoloxidase activating system, an enzyme cascade present in arthropod blood, has been shown to be involved in defense and recognition reactions. this system is converted to its active form by fungal 1,3-beta-d-glucans through binding to a plasma protein, a 1,3-beta-d-glucan-binding protein. here the molecular cloning and carbohydrate composition of the 1,3-beta-d-glucan-binding protein from the freshwater crayfish pacifastacus leniusculus are reported. it is also demonstrated that this ... | 1994 | 7961928 |
| opsonic activity of cell adhesion proteins and beta-1,3-glucan binding proteins from two crustaceans. | a beta-1,3-glucan binding protein (beta gbp) from the shore crab carcinus maenas was purified from plasma by precipitation of the protein at low ionic strength. the protein had a molecular mass of 110 kda, and was shown to affinity precipitate with laminarin, a soluble beta-1,3-glucan, and to cross-react with an antiserum directed toward beta gbp from the crayfish pacifastacus leniusculus. also, a protein from the haemocytes of c. maenas with a molecular mass of 80 kda was found to mediate cell ... | 1994 | 8050614 |
| characterization of a clotting protein, isolated from plasma of the freshwater crayfish pacifastacus leniusculus. | a protein responsible for clot formation was isolated from plasma of the crayfish pacifastacus leniusculus, by repeated precipitation at low ionic strength, ph 6.0. the protein, here named clotting protein (cp), is a lipoglycoprotein, which consists of two 210-kda subunits, covalently associated by disulfide bonds. preparations of the cp can form stable clots in the presence of crayfish haemocyte lysate supernatant, which contains endogenous, ca(2+)-dependent transglutaminase (tgase) activity. t ... | 1993 | 8097463 |
| isolation and characterization of a hemagglutinin with affinity for lipopolysaccharides from plasma of the crayfish pacifastacus leniusculus. | a hemagglutinin with a high specific activity against trypsinized rabbit erythrocytes was identified in plasma of the freshwater crayfish pacifastacus leniusculus. the activity of this crayfish hemagglutinin could be inhibited by sialoglycoproteins such as porcine stomach mucin, bovine submaxillary mucin, fetuin, and ovalbumin. however, the involvement of sialic acid in its binding specificity could not be unambiguously proven. furthermore, the hemagglutinating activity in the crayfish plasma co ... | 1993 | 8270093 |
| trace elements in crayfish: regional differences and changes induced by cooking. | the concentrations of pb, cd, zn, cu, ni, cr, mn, co, as, and se were determined in the abdominal muscle and hepatopancreas from raw and cooked crayfish of astacus astacus (l.) and pacifastacus leniusculus (dana), both of swedish origin. after cooking, the concentrations of cd, ni, and co in the hepatopancreas showed a decrease and the concentrations of pb, cu, and mn in the abdominal muscle showed an increase. metal and metalloid concentrations were also determined in the abdominal muscle and h ... | 1994 | 8311505 |
| purification and cdna cloning of ferritin from the hepatopancreas of the freshwater crayfish pacifastacus leniusculus. | ferritin was purified from the hepatopancreas of the freshwater crayfish pacifastacus leniusculus after injection of iron. it has the same size as horse spleen ferritin (440 kda) and migrates as two bands, 19 kda and 20 kda, respectively, in sds/page under reducing conditions. crayfish ferritin (20 kda) was cloned from a hepatopancreas cdna library. the deduced amino acid sequence of the crayfish ferritin shows a closer relationship to vertebrate ferritin heavy chains than to insect ferritin and ... | 1996 | 8612615 |
| effect of fixed epimastigote forms of trypanosoma cruzi on the hemocytes and the prophenoloxidase-activating system of the crayfish pacifastacus leniusculus. | the effect of the parasite trypanosoma cruzi on the hemocytes and the prophenoloxidase (propo)-activating system of the crayfish pacifastacus leniusculus was studied. incubation of the crayfish hemocyte lysate with fixed epimastigote forms of the parasites (4 x 10(5) cells/ml) induced a marked activation of the crayfish propo system, measured as phenoloxidase activity. the activation of propo by the parasite was much stronger (7-fold) than that induced by beta-1, 3-glucans (1 mg/ml) which are kn ... | 1996 | 9181104 |
| a very-high-density lipoprotein with clotting ability from hemolymph of sand crayfish, ibacus ciliatus. | a very-high-density lipoprotein (vhdl) with a density of 1.27-1.29 g/ml was the most abundant lipoprotein in the hemolymph of the sand crayfish ibacus ciliatus. the vhdl isolated by a density gradient ultracentrifugation consisted of 94% protein and 6% lipid reflecting its high density, and phospholipid was a predominant lipid component. the vhdl had an apolipoprotein of molecular mass 195 kda and its n-terminal amino acid sequence was identified as follows: lqpgleyqyryngrvaa. this sequence was ... | 1998 | 9571775 |
| a cell-surface superoxide dismutase is a binding protein for peroxinectin, a cell-adhesive peroxidase in crayfish. | peroxinectin, a cell-adhesive peroxidase (homologous to human myeloperoxidase), from the crayfish pacifastacus leniusculus, was shown by immuno-fluorescence to bind to the surface of crayfish blood cells (haemocytes). in order to identify a cell surface receptor for peroxinectin, labelled peroxinectin was incubated with a blot of haemocyte membrane proteins. it was found to specifically bind two bands of 230 and 90 kda; this binding was decreased in the presence of unlabelled peroxinectin. purif ... | 1999 | 10036241 |
| cell adhesion molecules in invertebrate immunity. | cell adhesion is essential in immunity in invertebrates, e.g., in the cellular immune responses of encapsulation and nodule formation. here cell adhesion molecules shown or suggested to be involved in invertebrate immunity are reviewed. blood cells of the crayfish, pacifastacus leniusculus, can release a cell-adhesive and opsonic peroxidase, peroxinectin. a site containing the motif, kgd, appears to be adhesive by binding to a transmembrane receptor of the integrin family on the blood cells. per ... | 1999 | 10426424 |
| the use of two species of crayfish as environmental quality sentinels: the relationship between heavy metal content, cell and tissue biomarkers and physico-chemical characteristics of the environment. | in systems as heavily disturbed as rivers, the use of biological sentinels is a most interesting way of obtaining continuous assessment of environmental quality. this study seeks to establish the value of such sentinels of two species of crayfish: the native crayfish (austropotamobius pallipes, lereboullet 1858) and an introduced species, signal crayfish (pacifastacus leniusculus, dana 1852), by comparing the habitat quality (water and sediments) and heavy metal contents in crayfish with the deg ... | 2000 | 10803551 |
| properties of the prophenoloxidase activating enzyme of the freshwater crayfish, pacifastacus leniusculus. | the prophenoloxidase activating enzyme (ppa), a serine proteinase catalyzing the conversion of prophenoloxidase to an active phenoloxidase, has a molecular mass of about 36 kda in its active form. this protein was cloned from a blood cell cdna library and its corresponding cdna of 1736 base pairs encodes a zymogenic protein (proppa) of 468 amino acids. an antibody raised against a synthetic peptide derived from a region of the cdna sequence could efficiently inhibit the beta-1,3-glucan triggered ... | 2001 | 11179955 |
| characterization of a pattern recognition protein, a masquerade-like protein, in the freshwater crayfish pacifastacus leniusculus. | a multifunctional masquerade-like protein has been isolated, purified, and characterized from hemocytes of the freshwater crayfish, pacifastacus leniusculus. it was isolated by its escherichia coli binding property, and it binds to formaldehyde-treated gram-negative bacteria as well as to yeast, saccharomyces cerevisiae, whereas it does not bind to formaldehyde-fixed gram-positive bacteria. the intact masquerade (mas)-like protein is present in crayfish hemocytes as a heterodimer composed of two ... | 2001 | 11390482 |
| a beta-1,3-glucan binding protein from the black tiger shrimp, penaeus monodon. | a beta-1,3-glucan binding protein (gbp) has been isolated from a shrimp hemocyte cdna library. its open reading frame consists of 1314 nucleotides with a polyadenylated sequence and a poly a tail. it encodes a polypeptide of 370 amino acids including a 17 amino acid-signal peptide. the mature protein has an estimated molecular mass of 39.5 kda and a predicted pi of 5.5. sequence comparison shows a high degree of similarity to invertebrate recognition proteins with glucanase-like domains for exam ... | 2002 | 11755673 |
| experimental infection of white spot syndrome virus in freshwater crayfish pacifastacus leniusculus. | the signal freshwater crayfish pacifastacus leniusculus was found to be susceptible to infection with white spot syndrome virus (wssv). histopathological observations of various tissues of virus-injected crayfish showed similar symptoms to those from wssv-infected penaeid shrimp, but no appearance of white spots on the cuticle or reddish body colour were observed, although these are the prominent gross signs of white spot disease in shrimp. a gene probe for detecting wssv was developed in order ... | 2001 | 11775797 |
| the environmental consequences of alien species in the swedish lakes mälaren, hjälmaren, vänern and vättern. | twenty alien species have become established in the lakes mälaren, vänern, vättern and hjälmaren. intentional introductions include fish and the signal crayfish from north america, ornamental plants, and the canada goose. unintentional introductions include the crayfish plague introduced with infected crayfish, the zebra mussel, and chinese mitten crab introduced with ballast water. the introduction of pathogens and parasites, in particular the crayfish plague, to the lakes has had the greatest ... | 2001 | 11878025 |
| host prophenoloxidase expression in freshwater crayfish is linked to increased resistance to the crayfish plague fungus, aphanomyces astaci. | the crayfish plague (aphanomyces astaci) susceptible freshwater crayfish astacus astacus and the resistant species pacifastacus leniusculus were compared with respect to differential haemocyte count and expression of prophenoloxidase and peroxinectin. a major difference found was that resistant crayfish continuously produced high levels of prophenoloxidase (propo) transcripts and that these levels could not be further increased, whereas in susceptible crayfish propo transcript levels and resista ... | 2003 | 12713493 |
| bacterial production and purification of sgpi-1 and sgpi-2, two peptidic serine protease inhibitors from the desert locust, schistocerca gregaria. | the last decade, a new serine protease inhibitor family has been described in arthropods. eight members were purified from the locusts locusta migratoria (lmpi-1-2 and hi) and schistocerca gregaria (sgpi-1-5) and 11 additional locust peptides were identified by cdna cloning. furthermore, the light chain of the 155-kda heterodimeric protease inhibitor pacifastin, from the freshwater crayfish pacifastacus leniusculus, was found to be composed of nine consecutive inhibitory domains (plds). these do ... | 2003 | 14550636 |
| molecular cloning and characterisation of a cell adhesion molecule, peroxinectin from the white shrimp litopenaeus vannamei. | expression of peroxinectin cdna was obtained from haemocytes of white shrimp litopenaeus vannamei using oligonucleotide primers based on the peroxinectin sequence of tiger shrimp penaeus monodon and freshwater crayfish pacifastacus leniusculus. the peroxinectin of l. vannamei was constitutively expressed. the cloning of peroxinectin cdna fragment was subjected to rt-pcr using degenerated primers. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nu ... | 2004 | 15145414 |
| effect of water temperature on the immune response and infectivity pattern of white spot syndrome virus (wssv) in freshwater crayfish. | the susceptibility of two species of freshwater crayfish, pacifastacus leniusculus and astacus astacus, to white spot syndrome virus (wssv) by intramuscular injection was compared and the results show that both species are susceptible to wssv. the effect of water temperature on the development of white spot disease in crayfish was also studied. crayfish were exposed to different temperatures after wssv injection or oral exposure and the mortalities were recorded over a period of 45 days. no mort ... | 2004 | 15276606 |
| physiological and genetic characterisation of some new aphanomyces strains isolated from freshwater crayfish. | five aphanomyces strains were isolated during suspected outbreaks of crayfish disease in spain and italy. genetic and physiological evidence show that the strains isolated from the freshwater crayfish procambarus clarkii and pacifastacus leniusculus, do not fit into any previously identified group of aphanomyces astaci and are not capable of killing crayfish following standardised experimental infection. rapd-pcr and its sequencing analysis show a high degree of similarity between the new isolat ... | 2004 | 15530744 |
| molecular cloning and characterisation of a pattern recognition molecule, lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) from the white shrimp litopenaeus vannamei. | a lipopolysaccharide- and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte and hepatopancreas of white shrimp litopenaeus vannamei using oligonucleotide primers and rt-pcr. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nucleotide sequence revealed that the cdna clone has an open reading frame of 1101 bp encoding a protein of 367 amino acids including a 17 amino acid signal peptide. the calculated molecular mass of the m ... | 2005 | 15561560 |
| molecular cloning and characterisation of prophenoloxidase from haemocytes of the white shrimp, litopenaeus vannamei. | cdna encoding prophenoloxidase (propo) of the white shrimp litopenaeus vannamei was obtained from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna using oligonucleotide primers based on the propo sequence of tiger shrimp penaeus monodon, freshwater crayfish pacifastacus leniusculus, green tiger shrimp penaeus semisulcatus (accession no.: af521949) and kuruma shrimp marsupenaeus japonicus (accession no.: ab0733223). propo of l. vannamei was ... | 2005 | 15683918 |
| an ancient role for a prokineticin domain in invertebrate hematopoiesis. | hemopoietic development requires firm control of cell proliferation and differentiation. although recent research has revealed conserved function of transcription factors and signaling pathways regulating lineage commitment in hemopoietic development in drosophila melanogaster and vertebrates, little is known about hemopoietic cytokines among the invertebrate phyla. in the present study, we show that differentiation and growth of hemopoietic stem cells in vitro from an invertebrate, pacifastacus ... | 2005 | 15879111 |
| molecular cloning and characterisation of copper/zinc superoxide dismutase (cu,zn-sod) from the giant freshwater prawn macrobrachium rosenbergii. | a copper/zinc superoxide dismutase (cu,zn-sod) cdna was cloned from the hepatopancreas of giant freshwater prawn macrobrachium rosenbergii using reverse transcription-polymerase chain reaction (rt-pcr) by degenerate primers. both 3'- and 5'-regions were isolated by the rapid amplification of cdna ends method. analysis of nucleotide sequence revealed that the cu,zn-sod cdna clone consists of 845 bp with an open reading frame of 603 bp encoding a protein of 201 amino acids with a 22 amino acid sig ... | 2006 | 16356736 |
| molecular cloning and characterisation of prophenoloxidase cdna from haemocytes of the giant freshwater prawn, macrobrachium rosenbergii, and its transcription in relation with the moult stage. | expression of prophenoloxidase (propo) cdna was determined from haemocytes of the giant freshwater prawn macrobrachium rosenbergii by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna using oligonucleotide primers based on the propo sequence of tiger shrimp penaeus monodon, freshwater crayfish pacifastacus leniusculus, green tiger shrimp penaeus semisulcatus, kuruma shrimp marsupenaeus japonicus, and white shrimp litopenaeus vannamei. the propo of m. rose ... | 2006 | 16376571 |
| molecular cloning and characterisation of peroxinectin, a cell adhesion molecule, from the giant freshwater prawn macrobrachium rosenbergii. | expression of peroxinectin cdna was determined from haemocytes of giant freshwater prawn macrobrachium rosenbergii using oligonucleotide primers and reverse transcription polymerase chain reaction (rt-pcr) based on the peroxinectin sequence of white shrimp litopenaeus vannamei, tiger shrimp penaeus monodon, and freshwater crayfish pacifastacus leniusculus. the peroxinectin of m. rosenbergii was constitutively expressed. analysis of the nucleotide sequence revealed that the cdna clone has an open ... | 2006 | 16377210 |
| characterization of white spot syndrome virus replication in in vitro-cultured haematopoietic stem cells of freshwater crayfish, pacifastacus leniusculus. | replication of white spot syndrome virus (wssv) was investigated in haematopoietic cells (hpt cells) derived from haematopoietic tissue (hpt) of freshwater crayfish, pacifastacus leniusculus. temperature and type of inoculum for virus replication were studied. the cell culture remained viable at a wide range of temperatures ranging from 4 to 25 degrees c. wssv replicated in cells, as evidenced by in situ hybridization, rt-pcr and by the presence of virions visualized with an electron microscope. ... | 2006 | 16528034 |
| characterization of a hemocyte intracellular fatty acid-binding protein from crayfish (pacifastacus leniusculus) and shrimp (penaeus monodon). | intracellular fatty acid-binding proteins (fabps) are small members of the superfamily of lipid-binding proteins, which occur in invertebrates and vertebrates. included in this superfamily are the cellular retinoic acid-binding proteins and retinol-binding proteins, which seem to be restricted to vertebrates. here, we report the cdna cloning and characterization of two fabps from hemocytes of the freshwater crayfish pacifastacus leniusculus and the shrimp penaeus monodon. in both these proteins, ... | 2006 | 16734719 |
| cloning and characterisation of a prophenoloxidase from the haemocytes of mud crab scylla serrata. | a prophenoloxidase (propo) cdna was cloned from the haemocytes of mud crab scylla serrata using oligonucleotide primers and rt-pcr. both 3'- and 5'-regions were isolated by rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the cdna clone has a full length of 2663bp, with an open reading frame of 2019bp, a 124-bp 5'-untranslated region, and a 520-bp 3'-untranslated region containing a poly a signal. it encodes a protein of 673 amino acids with a pred ... | 2007 | 16806468 |
| antilipopolysaccharide factor interferes with white spot syndrome virus replication in vitro and in vivo in the crayfish pacifastacus leniusculus. | in a study of genes expressed differentially in the freshwater crayfish pacifastacus leniusculus infected experimentally with the white spot syndrome virus (wssv), one protein, known as antilipopolysaccharide factor (alf), was chosen, among those whose transcript levels increased upon viral infection, for further studies. alf rna interference (rnai) experiments in whole animals and in cell cultures indicated that alf can protect against wssv infection, since knockdown of alf by rnai specifically ... | 2006 | 17041217 |
| antibacterial peptides in hemocytes and hematopoietic tissue from freshwater crayfish pacifastacus leniusculus: characterization and expression pattern. | a 14 amino acid residues proline/arginine-rich antibacterial peptide designated as astacidin 2 was purified and characterized from hemocytes of the freshwater crayfish, pacifastacus leniusculus. astacidin 2 has a broad range of antibacterial activity against both gram-positive and gram-negative bacteria. the primary sequence of astacidin 2 is rprpnyrprpiyrp with an amidated c-terminal and the molecular mass is 1838da determined by mass spectrometry. furthermore, the cdna of three different crust ... | 2007 | 17049601 |
| use of chemical communication in the management of freshwater aquatic species that are vectors of human diseases or are invasive. | chemical communication occurs when both originator (signaller) and one or more receiver(s) possess specializations for chemical exchange of information. chemical information can be used by a wide variety of species to locate food and mates, avoid predators and engage in social interactions. in this review, we focus on chemical signalling between mates or cues from nest sites or hosts by selected aquatic pest species and indicate how chemical information can be used to manage pests. the pests are ... | 2007 | 17367788 |
| phenoloxidase is an important component of the defense against aeromonas hydrophila infection in a crustacean, pacifastacus leniusculus. | the melanization cascade, in which phenoloxidase is the terminal enzyme, appears to play a key role in recognition of and defense against microbial infections in invertebrates. here, we show that phenoloxidase activity and melanization are important for the immune defense toward a highly pathogenic bacterium, aeromonas hydrophila, in the freshwater crayfish, pacifastacus leniusculus. rna interference-mediated depletion of crayfish prophenoloxidase leads to increased bacterial growth, lower phago ... | 2007 | 17855335 |
| identification of the extracellular copper-zinc superoxide dismutase (eccuznsod) gene of the mud crab scylla serrata and its expression following beta-glucan and peptidoglycan injections. | an extracellular copper-zinc superoxide dismutase (eccuznsod, or ec-sod) gene was cloned from haemocytes of mud crab scylla serrata. the eccuznsod cdna consists of 939 bp with an open reading frame (orf) of 585 bp that encodes 195 amino acids (aa) with 31 residues of a signal peptide sequence. the predicted molecular mass of the mature protein (164aa) is 17.1 kda with an estimated pi of 6.89. amino acids responsible for binding cu (his84, 86, 101, and 163) and zn (his101, 109, and 121, and asp 1 ... | 2008 | 17949815 |
| molecular cloning and characterisation of a pattern recognition protein, lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) from chinese shrimp fenneropenaeus chinensis. | a pattern recognition protein (prp), lipopolysaccharide and beta-1,3-glucan binding protein (lgbp) cdna was cloned from the haemocyte of chinese shrimp fenneropenaeus chinensis by the techniques of homology cloning and race. analysis of nucleotide sequence revealed that the full-length cdna of 1,275 bp has an open reading frame of 1,098 bp encoding a protein of 366 amino acids including a 17 amino acid signal peptide. sequence comparison of the deduced amino acid sequence of f. chinensis lgbp sh ... | 2009 | 18163220 |
| sifamide peptides in clawed lobsters and freshwater crayfish (crustacea, decapoda, astacidea): a combined molecular, mass spectrometric and electrophysiological investigation. | recently, we identified the peptide vyrkppfngsifamide (val(1)-sifamide) in the stomatogastric nervous system (stns) of the american lobster homarus americanus using matrix-assisted laser desorption/ionization-fourier transform mass spectrometry (maldi-ftms). given that h. americanus is the only species thus far shown to possess this peptide, and that a second sifamide isoform, gly(1)-sifamide, is broadly conserved in other decapods, including another astacidean, the crayfish procambarus clarkii, ... | 2008 | 18308319 |
| a serpin from chinese shrimp fenneropenaeus chinensis is responsive to bacteria and wssv challenge. | arthropod defence responses (e.g. prophenoloxidase (propo) activation and toll pathway initiation) are mediated by serine proteinase cascades and regulated by serpins in haemolymph. a serpin (fc-serpin) cdna was cloned from the haemocytes of fenneropenaeus chinensis by rapid amplification of cdna ends (race) pcr and haemocyte cdna library screening. the full-length cdna consists of 1734bp, encoding 411 amino acids with a calculated molecular mass of 46.55kda and a theoretical isoelectric point o ... | 2009 | 18817877 |
| genetic identification of mammalian carnivore species in the kushiro wetland, eastern hokkaido, japan, by analysis of fecal dna. | to identify mammalian carnivore species distributed in the kushiro wetland, eastern hokkaido, japan, we developed molecular-genetic methods for identification of the species from fecal samples collected from the field. species-specific primers and pcr programs were established for five native and six alien species of carnivores: martes zibellina, mustela nivalis, mustela erminea, vulpes vulpes, and nyctereutes procyonoides as native species, and neovison vison, martes melampus, mustela itatsi, c ... | 2008 | 18828658 |
| a quantitative taqman mgb real-time polymerase chain reaction based assay for detection of the causative agent of crayfish plague aphanomyces astaci. | here we present the development and first validation of a taqman minor groove binder (mgb) real-time polymerase chain reaction (rt-pcr) method for quantitative and highly specific detection of aphanomyces astaci, the causative agent of crayfish plague. the assay specificity was experimentally assessed by testing against dna representative of closely related oomycetes, and theoretically assessed by additional sequence similarity analyses comparing the primers and probe sequences to available sequ ... | 2009 | 19201113 |
| a highly virulent pathogen, aeromonas hydrophila, from the freshwater crayfish pacifastacus leniusculus. | aeromonas spp. are characteristic bacteria of freshwaters and many of them can be components of the bacterial flora of aquatic animals and may become pathogens on animals including humans. in this study aeromonas hydrophila was isolated from the freshwater crayfish, pacifastacus leniusculus, and was found to be a highly pathogenic bacterium among many isolated bacteria. mortality reached 100% within 6h when 200 microl of 1.24 x 10(7)cfu/ml was applied by injection. histopathological studies of m ... | 2009 | 19233188 |
| role of anti-lipopolysaccharide factor from the black tiger shrimp, penaeus monodon, in protection from white spot syndrome virus infection. | the anti-lipopolysaccharide factor (alf) from the black tiger shrimp, penaeus monodon, has been shown previously to exhibit a broad spectrum of activity against various strains of bacteria and fungi. herein, the recombinant alfpm3 (ralfpm3) protein was examined for its role in the defence against white spot syndrome virus (wssv) infection in haematopoietic (hpt) cell cultures of the freshwater crayfish, pacifastacus leniusculus, as well as in live p. monodon shrimps. incubation of hpt cell cultu ... | 2009 | 19264668 |
| gene silencing of a prophenoloxidase activating enzyme in the shrimp, penaeus monodon, increases susceptibility to vibrio harveyi infection. | the prophenoloxidase (propo) activating system is an important innate immune response against microbial infections in invertebrates. the major enzyme, phenoloxidase (po), is synthesized as an inactive precursor and its activation to an active enzyme is mediated by a cascade of clip domain serine proteinases (clip-sps). in this study, a cdna encoding a propo activating enzyme (ppae) from the black tiger shrimp, penaeus monodon, designated as pmppae1, was cloned and characterized. the full-length ... | 2009 | 19428482 |
| prevalence of the crayfish plague pathogen aphanomyces astaci in invasive american crayfishes in the czech republic. | in central europe invasive north american crayfishes are carriers of the oomycete aphanomyces astaci, which causes crayfish plague. this lethal disease currently represents one of the major threats to native european crayfishes. we used molecular methods-species--specific amplification and sequencing of the pathogen dna--to investigate the prevalence of individuals latently infected with a. astaci in 28 populations of two invasive american crayfish species (6 of the signal crayfish [pacifastacus ... | 2009 | 19459897 |
| high sequence variability among hemocyte-specific kazal-type proteinase inhibitors in decapod crustaceans. | crustacean hemocytes were found to produce a large number of transcripts coding for kazal-type proteinase inhibitors (kpis). a detailed study performed with the crayfish pacifastacus leniusculus and the shrimp penaeus monodon revealed the presence of at least 26 and 20 different kazal domains from the hemocyte kpis, respectively. comparisons with kpis from other taxa indicate that the sequences of these domains evolve rapidly. a few conserved positions, e.g. six invariant cysteines were present ... | 2010 | 19715720 |
| cdna cloning, identification, tissue localisation, and transcription profile of a transglutaminase from white shrimp, litopenaeus vannamei, after infection by vibrio alginolyticus. | complementary (c)dna encoding transglutaminase (tg) messenger (m)rna of white shrimp, litopenaeus vannamei, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the tg sequence of the horseshoe crab, tachypleus tridentatus (accession no.: baa02134); tiger shrimp, penaeus monodon (aal78166); and pacifastacus leniusculus (af336805). the 2638-bp cdna contained an open reading frame ... | 2009 | 19782141 |
| in vitro effects on bacterial growth of phenoloxidase reaction products. | an active phenoloxidase preparation from the freshwater crayfish pacifastacus leniusculus exhibited a strong antibacterial effect in vitro on the bacteria aeromonas hydrophila, escherichia coli, streptococcus pneumoniae whereas a weaker but still significant effect against bacillus cereus, pseudomonas aeruginosa and staphylococcus aureus. in most cases reduction of bacterial growth was stronger when dopamine was used as substrate as compared to l-dopa. the effect on bacteria was abolished if no ... | 2010 | 19808037 |
| expression of immune-related genes in one phase of embryonic development of freshwater crayfish, pacifastacus leniusculus. | crayfish do not have larval stage as other crustacean such as penaeid shrimp they spawn their eggs until hatching and what hatches out from the eggs are miniature crayfish known as juveniles. in order to address the question whether immune genes are initially expressed during the embryo development in the egg stage, the expression of some immune-related genes: prophenoloxidase (propo), peroxinectin, hemocyanin, anti-lipopolysaccharide factor (alf), plcrustin, astakine-1, 2 and transglutaminase ( ... | 2010 | 20060476 |
| characterization of two crustin antimicrobial peptides from the freshwater crayfish pacifastacus leniusculus. | the two bacteria-induced crustin genes, plcrustin1 and plcrustin2, previously found in the hemocyte cdna library of pacifastacus leniusculus, contain the open reading frames of 357 bp encoding a putative protein of 118 amino acid residues and 330 bp encoding a putative protein of 109 amino acid residues, respectively. the carboxyl-terminal part of the two crustins possesses, respectively, 7 and 8 conserved cysteine residues representation of a wap domain that is found in carcinins and crustins i ... | 2010 | 20371367 |
| a novel viral responsive protein is involved in hemocyte homeostasis in the black tiger shrimp, penaeus monodon. | a novel viral responsive protein, namely hemocyte homeostasis-associated protein (hhap), was characterized for its role in the response of shrimp to white spot syndrome virus infection. the full-length cdnas of hhap from the black tiger shrimp (pmhhap), penaeus monodon, and the fresh water crayfish (plhhap), pacifastacus leniusculus, were obtained and showed high sequence identity to a hypothetical protein from various organisms, with the highest identity to the hypothetical protein tcasga2_tc00 ... | 2010 | 20444692 |
| trophic transfer of aluminium through an aquatic grazer-omnivore food chain. | the potential for trophic transfer of aluminium (al) was investigated using a grazing detritivore, the freshwater snail lymnaea stagnalis, and a predator, the signal crayfish pacifastacus leniusculus. snails were exposed to either aqueous al (500 microg l(-1)) in the presence or absence of an inorganic ligand (phosphate (+p); 500 microg l(-1)) for 30 days, or kept as unexposed controls. subcellular partitioning of al in the snail tissues was characterised using ultracentrifugation. al content in ... | 2010 | 20451262 |
| cloning and characterization of a melanization inhibition protein (pmmip) of the black tiger shrimp, penaeus monodon. | melanization is an important component of the innate immune responses in invertebrates and it is essential for defense against invading microorganism. melanin formation, which is a result of activation of the so called prophenoloxidase activating system, needs to be controlled due to the dangerous effects of quinones and melanin which are produced during the process of melanization. here, a cdna for a melanization inhibition protein (mip), named pmmip, was identified from the black tiger shrimp, ... | 2010 | 20561997 |
| proteinase inhibitory activities of two two-domain kazal proteinase inhibitors from the freshwater crayfish pacifastacus leniusculus and the importance of the p(2) position in proteinase inhibitory activity. | serine proteinase inhibitors are found ubiquitously in living organisms and involved in homeostasis of processes using proteinases as well as innate immune defense. two two-domain kazal-type serine proteinase inhibitors (kpis), kpi2 and kpi8, have been identified from the hemocyte cdna library of the crayfish pacifastacus leniusculus. unlike other kpis from p. leniusculus, they are found specific to the hemocytes and contain an uncommon p(2) amino acid residue, gly. to unveil their inhibitory ac ... | 2010 | 20621193 |
| molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab portunus trituberculatus. | serine proteinase homologues (sphs), as one of prophenoloxiase-activating factors (ppafs), play critical roles in innate immunity of crabs. based on an est from the eyestalk full length cdna library, the complete cdna (designated as ptsph) and genomic dna of sph from the swimming crab portunus trituberculatus were cloned in this study. the estimated molecular weight of mature ptsph (354 amino acids) was 38.7 kda and its isoelectric point was 5.08. multiple sequence alignment revealed that ptsph ... | 2010 | 20633654 |
| a gc1qr prevents white spot syndrome virus replication in the freshwater crayfish pacifastacus leniusculus. | the gc1qr/p32 protein is a multiple receptor for several proteins and pathogens. we cloned a gc1qr homologue in a crustacean, pacifastacus leniusculus, and analyzed the expression of p. leniusculus c1qr (plgc1qr) in various tissues. the gc1qr/p32 transcript was significantly enhanced by white spot syndrome virus (wssv) infection 6 h after viral infection both in vitro in a hematopoietic tissue cell culture (hpt) and in vivo compared to appropriate controls. moreover, plgc1qr silencing in both th ... | 2010 | 20686021 |
| molecular characterization and expression analysis of extracellular copper-zinc superoxide dismutase gene from swimming crab portunus trituberculatus. | an extracellular cuznsod cdna was cloned from the haemocytes of swimming crab portunus trituberculatus by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) method. analysis of the nucleotide sequence revealed that the eccuznsod full-length cdna consisted of 965 bp with an open reading frame of 579 bp. it encoded a polypeptide of 192 amino acids which had a predicted molecular weight of 20.0 kda and with an estimated pi of 6.23. the deduced amin ... | 2011 | 20848212 |
| melanization and pathogenicity in the insect, tenebrio molitor, and the crustacean, pacifastacus leniusculus, by aeromonas hydrophila ah-3. | aeromonas hydrophila is the most common aeromonas species causing infections in human and other animals such as amphibians, reptiles, fish and crustaceans. pathogenesis of aeromonas species have been reported to be associated with virulence factors such as lipopolysaccharides (lps), bacterial toxins, bacterial secretion systems, flagella, and other surface molecules. several mutant strains of a. hydrophila ah-3 were initially used to study their virulence in two animal species, pacifastacus leni ... | 2010 | 21206752 |
| permanent genetic resources added to molecular ecology resources database 1 october 2010-30 november 2010. | this article documents the addition of 277 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: ascochyta rabiei, cambarellus chapalanus, chionodraco hamatus, coptis omeiensis, cynoscion nebulosus, daphnia magna, gerbillus nigeriae, isurus oxyrinchus, lates calcarifer, metacarcinus magister, oplegnathus fasciatus, pachycondyla verenae, phaethon lepturus, pimelodus grosskopfii, rotylenchulus reniformis, scomberomorus niphonius, sep ... | 2011 | 21429157 |
| permanent genetic resources added to molecular ecology resources database 1 december 2010-31 january 2011. | this article documents the addition of 238 microsatellite marker loci to the molecular ecology resources database. loci were developed for the following species: alytes dickhilleni, arapaima gigas, austropotamobius italicus, blumeria graminis f. sp. tritici, cobitis lutheri, dendroctonus ponderosae, glossina morsitans morsitans, haplophilus subterraneus, kirengeshoma palmata, lysimachia japonica, macrolophus pygmaeus, microtus cabrerae, mytilus galloprovincialis, pallisentis (neosentis) celatus, ... | 2011 | 21457476 |
| involvement of peroxinectin in the defence of red swamp crayfish procambarus clarkii against pathogenic aeromonas hydrophila. | cell adhesion factors are important immune components for invertebrate to immobilize, phagocytose or encapsulate invasive microorganisms and foreign particles. in this study, a new cell adhesion factor, peroxinectin (refered as pcpxin) was isolated from hemocytes of red swamp crayfish (procambarus clarkii). the full-length cdna of pcpxin was 3014 bp encoding a protein of 819 amino acid residues with a predicted molecular weight of 89.0 kda and a calculational isoelectric point of 6.93. the putat ... | 2011 | 21539922 |
| two novel ficolin-like proteins act as pattern recognition receptors for invading pathogens in the freshwater crayfish pacifastacus leniusculus. | to isolate pathogen-associated molecular patterns (pamps)-binding molecules, the bacterium, staphylococcus aureus was used as an affinity matrix to find bacteria-binding proteins in the plasma of the freshwater crayfish, pacifastacus leniusculus. two new bacteria-binding ficolin-like proteins (flps) were identified by 2-de and ms analysis. the flps have a fibrinogen-related domain (fred) in their c-terminal and a repeat region in their n-terminal regions with putative structural similarities to ... | 2011 | 21598394 |
| detection and quantification of the crayfish plague agent in natural waters: direct monitoring approach for aquatic environments. | aphanomyces astaci, a specialised parasite of north american freshwater crayfish, is the disease agent of crayfish plague that is lethal to european freshwater crayfish. the life cycle of a. astaci has been inferred from experimental laboratory studies, but less is known about its natural sustainability and ecology. to address such questions, tools for monitoring of a. astaci directly in aquatic environments are needed. here, we present an approach for detecting and quantifying a. astaci directl ... | 2011 | 21797031 |
| bacteria-induced dscam isoforms of the crustacean, pacifastacus leniusculus. | the down syndrome cell adhesion molecule, also known as dscam, is a member of the immunoglobulin super family. dscam plays an essential function in neuronal wiring and appears to be involved in innate immune reactions in insects. the deduced amino acid sequence of dscam in the crustacean pacifastacus leniusculus (pldscam), encodes 9(ig)-4(fniii)-(ig)-2(fniii)-tm and it has variable regions in the n-terminal half of ig2 and ig3 and the complete ig7 and in the transmembrane domain. the cytoplasmic ... | 2011 | 21695245 |
| an insect tep in a crustacean is specific for cuticular tissues and involved in intestinal defense. | in an attempt to identify genes encoding thioester-containing proteins in the freshwater crayfish, pacifastacus leniusculus, three different cdnas were found. a phylogenetic analysis of these proteins indicates that they can be classified into two subfamilies: two alpha-2-macroglobulins (pl-a2m1, pl-a2m2) showing a close similarity to shrimp a2m, and one insect tep-like protein (pl-tep). this is the first report of an insect tep-like protein in a crustacean. crayfish pl-a2m1, pl-a2m2 and pl-tep ... | 2011 | 22193393 |
| Identification and cloning of a transglutaminase from giant freshwater prawn, Macrobrachium rosenbergii, and its transcription during pathogen infection and moulting. | Complementary (c)DNA encoding transglutaminase (TG) messenger (m)RNA of the giant freshwater prawn, Macrobrachium rosenbergii, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) using oligonucleotide primers based on the TG sequence of the horseshoe crab, Tachypleus tridentatus; tiger shrimp, Penaeus monodon; kuruma shrimp, Marsupenaeus japonicus; and crayfish, Pacifastacus leniusculus. The 2722-bp cDNA contained a ... | 2011 | 21854853 |
| re-examination of the prevalence of aphanomyces astaci in north american crayfish populations in central europe by taqman mgb real-time pcr. | we applied quantitative taqman minor groove binder real-time polymerase chain reaction (pcr) on dna isolates from soft abdominal cuticle of 460 north american crayfish orconectes limosus and pacifastacus leniusculus, previously tested for aphanomyces astaci presence by conventional semi-nested pcr. both approaches target the internal transcribed spacers of the pathogen nuclear ribosomal dna, but apply different specific sequence motifs and technologies. the real-time pcr approach seems to provid ... | 2011 | 22303628 |
| potent infection reservoir of crayfish plague now permanently established in norway. | noble crayfish astacus astacus is threatened in europe due to invasive crayfish carrying the crayfish plague agent aphanomyces astaci. norway is among the last countries in which the introduction of non-indigenous crayfish has been limited through strict legislation practices. however, north american signal crayfish pacifastacus leniusculus were recently discovered in a water-course that has been repeatedly hit by the plague. we mapped the distribution and relative density (catch per unit effort ... | 2011 | 22235597 |
| potential-dependent potassium currents in the slowly adapting stretch receptor neuron of the crayfish. | the outward current in the stretch receptor neuron of the crayfish pacifastacus leniusculus was analysed using a two-micropipette potential-clamp technique. the outward current was shown to be carried by k+. when the sodium-dependent inward current was blocked by tetrodotoxin a fast-activating potassium current was revealed, resembling the delayed rectifier. the time-course of activation (tau n) was dependent on potential and had a mean value of i ms at potential steps of to mv. the activation f ... | 2008 | 2596334 |
| ancient cytokines, the role of astakines as hematopoietic growth factors. | hematopoiesis is the process by which hemocytes mature and subsequently enter the circulation. vertebrate prokineticins (pks) are known to take part in this process, as are the invertebrate prokineticin domain proteins, astakines. in pacifastacus leniusculus, astakine 1 is essential for the release of new hemocytes into the open circulatory system of these animals. in addition to astakine 1, we have now cloned a homologue of astakine 1 with an insert of 13 amino acids, named as astakine 2. both ... | 2010 | 20592028 |
| characterization of a hemocyte homeostasis-associated-like protein (hhap) in the freshwater crayfish pacifastacus leniusculus. | hemocyte homeostasis-associated-like protein (hhap) in the freshwater crayfish pacifastacus leniusculus has a distinct role from that of its homolog pmhhap in the shrimp penaeus monodon. knockdown of plhhap in vitro using double-stranded rna (dsrna) had no effect on the cell morphology of hematopoietic tissue (hpt) cells. the total hemocyte number and caspase activity were unchanged after plhhap knockdown in vivo, in contrast to the results found in shrimp. moreover, suppression of plhhap both i ... | 2016 | 27663854 |
| the receptor for activated c kinase 1 (rack1) functions in hematopoiesis through jnk activation in chinese mitten crab eriocheir sinensis. | receptor for activated c kinase 1 (rack1) is a wd-domain repeating protein which involves in the mediation of various biological processes, including innate immune response. in the present study, a rack1 (designed as esrack1) gene from chinese mitten crab e. sinensis was cloned by rapid amplification of cdna ends (race) technique. the full-length cdna sequence of esrack1 was of 1117 bp with an open reading frame (orf) of 957 bp encoding a polypeptide of 318 amino acids containing seven wd repeat ... | 2016 | 27542616 |
| antifungal activity and pore-forming mechanism of astacidin 1 against candida albicans. | in a previous report, a novel antibacterial peptide astacidin 1 (fkvqnqhgqvvkifhh) was isolated from hemocyanin of the freshwater crayfish pacifastacus leniusculus. in this study, the antifungal activity and mechanism of astacidin 1 were evaluated. astacidin 1 exhibited antifungal activity against candida albicans, trichosporon beigelii, malassezia furfur, and trichophyton rubrum. also, astacidin 1 had fungal cell selectivity in human erythrocytes without causing hemolysis. to understand the ant ... | 2014 | 24955933 |
| identification and characterization of a serine protease inhibitor esserpin from the chinese mitten crab eriocheir sinensis. | serine protease inhibitors (serpins) represent an expanding superfamily of endogenous inhibitors that regulate proteolytic events and involve in a variety of physiological processes. a serine protease inhibitor, namely esserpin, was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) analysis. the full-length cdna of esserpin was of 2367 bp, including an open reading frame (orf) of 1371 bp encoding a polypeptide of 456 amino acids with estimated molecular ... | 2013 | 23567854 |
| a new type antimicrobial peptide astacidin functions in antibacterial immune response in red swamp crayfish procambarus clarkii. | a new antibacterial peptide called astacidin was characterized from hemocytes of red swamp crayfish procambarus clarkii, and designated as pcast. the full-length cdna of pcast contained 828 nucleotides with a polyadenylation sequence and a poly-a tail. pcast encoded a peptide of 43 amino acids, with a signal peptide of 23 amino acids. the mature peptide contained 20 amino acids, among which four were proline/arginine amino acids. similarity analysis showed that pcast shared high identity with as ... | 2014 | 24263004 |
| predatory functional response and prey choice identify predation differences between native/invasive and parasitised/unparasitised crayfish. | invasive predators may change the structure of invaded communities through predation and competition with native species. in europe, the invasive signal crayfish pacifastacus leniusculus is excluding the native white clawed crayfish austropotamobius pallipes. | 2012 | 22359673 |
| horizontal transmission of thelohania contejeani in the endangered white-clawed (austropotamobius pallipes) and the invasive signal crayfish (pacifastacus leniusculus). | the microsporidian parasite thelohania contejeani causes porcelain disease and has been implicated in mass mortalities in populations of the endangered european crayfish austropotamobius pallipes. however, the route of parasite transmission is not known. this paper investigates the horizontal transmission of t. contejeani between a. pallipes hosts as well as its transmissibility to the invasive signal crayfish (pacifastacus leniusculus). field collected juvenile a. pallipes and p. leniusculus we ... | 2012 | 22814084 |
| identification and cloning of the second type transglutaminase from litopenaeus vannamei, and its transcription following pathogen infection and in relation to the haemolymph coagulation. | complementary (c)dna encoding transglutaminaseii (tgii) messenger (m)rna of white shrimp, litopenaeus vannamei, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the tg sequence of the horseshoe crab, tachypleus tridentatus (accession no.: baa02134), tiger shrimp, penaeus monodon (aav49005; aao33455), kuruma shrimp, marsupenaeus japonicus (bad36808) and pacifastacus leniuscul ... | 2013 | 24036331 |
| a cytokine-like factor astakine accelerates the hemocyte production in pacific oyster crassostrea gigas. | astakine has been reported to be a hematopoietic growth factor of prokineticin homolog firstly found in arthropods freshwater crayfish pacifastacus leniusculus. in the present study, an astakine homologous gene was identified from pacific oyster crassostrea gigas (designated cgastakine). the full length cdna of cgastakine encoded a polypeptide of 103 amino acids containing a prokineticin (pk) domain homologous to that in astakine from freshwater crayfish p. leniusculus. the deduced amino acid se ... | 2016 | 26523496 |
| elements in fish of malibu creek and malibu lagoon near los angeles, california. | our aim was to assess whether past discharges from a wastewater treatment plant increased metal pollutant loads in stream mobile species in a one-day baseline sampling study that included a coastal wetland. mosquitofish (gambusia affinis) of two sizes, black bullhead (ameiurus melas), and crayfish (pacifastacus leniusculus) were collected from malibu creek, and california killifish (fundulus parvipinnis) of three sizes, as well as arroyo chub (gila orcutti) were sampled from malibu lagoon near l ... | 2003 | 12705915 |
| the significance of droughts for hyporheic dwellers: evidence from freshwater crayfish. | freshwater biodiversity is globally threatened by various factors while severe weather events like long-term droughts may be substantially devastating. in order to remain in contact with the water or stay in a sufficiently humid environment at drying localities, the ability to withstand desiccation by dwelling in the hyporheic zone, particularly through vertical burrowing is crucial. we assessed the ability of three european native and five non-native crayfish as models to survive and construct ... | 2016 | 27225308 |
| prevalence of the pathogen aphanomyces astaci in freshwater crayfish populations in croatia. | the oomycete aphanomyces astaci is an obligate crayfish parasite that co-evolved with american crayfish species, and they therefore generally live in a balanced relationship. on the contrary, european native crayfish are highly susceptible to a. astaci, and infestation with it causes development of the lethal disease termed crayfish plague. until now, 5 a. astaci strains have been described from the freshwater crayfish present in europe. in this study we aimed to investigate the occurrence of th ... | 2016 | 26865234 |
| effect of experimental exposure to differently virulent aphanomyces astaci strains on the immune response of the noble crayfish astacus astacus. | european crayfish are sensitive to the crayfish plague pathogen, aphanomyces astaci, carried by north american crayfish species due to their less effective immune defence mechanisms against this disease. during a controlled infection experiment with a susceptible crayfish species astacus astacus using three a. astaci strains (representing genotype groups a, b, and e), we investigated variation in their virulence and in crayfish immune defence indicators (haemocyte density, phenoloxidase activity ... | 2015 | 26410255 |
| molecular detection and genotyping of aphanomyces astaci directly from preserved crayfish samples uncovers the norwegian crayfish plague disease history. | aphanomyces astaci causes crayfish plague in european freshwater crayfish, but most historical epizootics lack agent isolation and identification. although declared as crayfish plague outbreaks by the norwegian competent authorities, only presumptive diagnoses without agent isolation exist from norwegian epizootics until 2005. molecular methods now allow both a. astaci detection and genotype determination from preserved samples. we therefore aimed to (1) investigate molecularly if a. astaci was ... | 2014 | 25124447 |
| distribution and epidemiology of genotypes of the crayfish plague agent aphanomyces astaci from noble crayfish astacus astacus in finland. | the crayfish plague agent aphanomyces astaci was isolated from 69 noble crayfish astacus astacus samples in finland between 1996 and 2006. all isolates were genotyped using randomly amplified polymorphic dna-polymerase chain reaction (rapd-pcr). altogether, 43 isolates belonged to the genotype group of astacus strains (as), which is assumed to represent the genotype originally introduced into europe around 1860 and into finland in 1893. there were 26 crayfish plague isolates belonging to the gro ... | 2013 | 23574706 |
| aphanomyces astaci in wild crayfish populations in slovenia: first report of persistent infection in a stone crayfish austropotamobius torrentium population. | all 5 crayfish species inhabiting slovenian freshwaters, of which 3 are indigenous crayfish species (ics: astacus astacus, austropotamobius pallipes, and a. torrentium) and 2 are non-indigenous (nics: pacifastacus leniusculus and cherax quadricarinatus), were inspected for the presence of aphanomyces astaci, the causative agent of crayfish plague. wild crayfish populations showing no clinical signs of infection were inspected using a. astaci-specific real-time pcr. in addition, a conventional pc ... | 2013 | 23548366 |
| comparative ultrastructure of the spermatozoa of three crayfish species: austropotamobius torrentium, pacifastacus leniusculus, and astacus astacus (decapoda: astacidae). | this study reports about the spermatozoal ultrastructure of three species of astacid crayfish, i.e., the stone crayfish austropotamobius torrentium, signal crayfish pacifastacus leniusculus, and noble crayfish astacus astacus. the acrosome is a cup shaped and electron-dense structure at the anterior of the spermatozoon and comprises three layers of differing electron densities filled with parallel filaments that extend from the base to the apical zone. the acrosome was significantly longer in a. ... | 2013 | 23475696 |
| incomes, attitudes, and occurrences of invasive species: an application to signal crayfish in sweden. | this article analyzes and carries out an econometric test of the explanatory power of economic and attitude variables for occurrences of the nonnative signal crayfish in swedish waters. signal crayfish are a carrier of plague which threatens the native noble crayfish with extinction. crayfish are associated with recreational and cultural traditions in sweden, which may run against environmental preferences for preserving native species. econometric analysis is carried out using panel data at the ... | 2009 | 18946699 |
| mechanotransduction and the crayfish stretch receptor. | mechanotransduction or mechanosensitivity is found in almost every cell in all organisms from bacteria to vertebrates. mechanosensitivity covers a wide spectrum of functions from osmosensing, cell attachment, classical sensory mechanisms like tactile senses in the skin, detection of sound in hair cells of the hearing apparatus, proprioceptive functions like recording of muscle length and tension in the muscle spindle and tendon organ, respectively, and pressure detection in the circulation etc. ... | 2007 | 17612581 |
| interactions among juveniles of two freshwater crayfish species and a predatory fish. | two freshwater crayfish species, astacus astacus l. and pacifastacus leniusculus dana, co-occur in some swedish lakes. observational studies indicate that the introduced, north american species p. leniusculus may gradually replace the native a. astacus, but the mechanism behind the replacement is not known. this study examined the direct effects of interspecific competition between the crayfish, and indirect effects of competitive interactions and fish (european perch, perca fluviatilis l.) pred ... | 1994 | 28307005 |
| the effect of repeated stimulation on the escape reflex and the acetylcholinesterase activity in different parts of the central nervous system in the crayfish (pacifastacus leniusculus and astacus astacus). | | 1974 | 4156203 |
| invasive crayfish and crayfish plague on the move: first detection of the plague agent aphanomyces astaci in the romanian danube. | native european crayfish, such as astacus leptodactylus, are threatened, among other factors, by the crayfish plague agent aphanomyces astaci, dispersed by invasive north american crayfish. two of these invaders, pacifastacus leniusculus and orconectes limosus, have extended their distribution in the river danube catchment; the latter was detected for the first time in romania in 2008. we monitored, at monthly intervals for over 2 yr, occurrence of native a. leptodactylus and invasive o. limosus ... | 2012 | 22422132 |
| the prevalence of aphanomyces astaci in invasive signal crayfish from the uk and implications for native crayfish conservation. | the crayfish plague agent, aphanomyces astaci, has spread throughout europe, causing a significant decline in native european crayfish. the introduction and dissemination of this pathogen is attributed to the spread of invasive north american crayfish, which can act as carriers for a. astaci. as native european crayfish often succumb to infection with a. astaci, determining the prevalence of this pathogen in non-native crayfish is vital to prioritize native crayfish populations for managed trans ... | 2017 | 28077183 |
| prevalence of the crayfish plague pathogen aphanomyces astaci in populations of the signal crayfish pacifastacus leniusculus in france: evaluating the threat to native crayfish. | aphanomyces astaci, the crayfish plague pathogen, first appeared in europe in the mid-19(th) century and is still responsible for mass mortalities of native european crayfish. the spread of this parasite across the continent is especially facilitated by invasive north american crayfish species that serve as its reservoir. in france, multiple cases of native crayfish mortalities have been suggested to be connected with the presence of the signal crayfish pacifastacus leniusculus, which is highly ... | 2013 | 23894606 |
| susceptibility to infection and pathogenicity of white spot disease (wsd) in non-model crustacean host taxa from temperate regions. | despite almost two decades since its discovery, white spot disease (wsd) caused by white spot syndrome virus (wssv) is still considered the most significant known pathogen impacting the sustainability and growth of the global penaeid shrimp farming industry. although most commonly associated with penaeid shrimp farmed in tropical regions, the virus is also able to infect, cause disease and kill a wide range of other decapod crustacean hosts from temperate regions, including lobsters, crabs, cray ... | 2012 | 22484233 |