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primary structure of an analog of crustacean pigment-dispersing hormone from the lubber grasshopper romalea microptera.an octadecapeptide capable of inducing pigment dispersion in the chromatophores of the fiddler crab uca pugilator has been isolated from lyophilized heads of the lubber grasshopper romalea microptera. this pigment-dispersing factor (pdf) was purified by gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase high performance liquid chromatography. automated gas-phase sequencing, followed by the identification of the carboxyl-terminal amide, established the prima ...19873818616
black spot gill syndrome of the northern shrimp pandalus borealis. 19744412217
microbiological evaluation of cold-water shrimp (pandalus borealis).a bacteriological survey of the maine shrimp industry was conducted to investigate the conditions associated with the production of frozen, raw, peeled shrimp. in-plant samples and finished product units were collected from seven plants. the most probable number of escherichia coli, coliforms, and coagulase-positive staphylococci, as well as aerobic plate counts (apc), were determined. freshly harvested shrimp collected from fishing vessels had an apc geometric mean of 510/g, and e. coli, colifo ...19734577485
[quality changes in iced shrimps (pandalus borealis). i. changes in the contents of trimethylamine oxide and volatile nitrogen bases and bacteria in raw shrimps after different storage periods compared with organoleptic examinations (author's transl)].on the basis of two experiments on the storage of raw shrimps (pandalus borealis) in ice and on samples taken out from different trawlers after different days at sea, we have attempted to find the criteria of choice for the lower limit of quality of raw shrimps which are meant for further processing for human consumption. a content of tvn of approximately 50 mg/100 g peeled shrimp and a content of tma-n of approximately 10 mg/100 g peeled shrimp probably represent such an objective lower limit i ...19817033925
[quality changes in iced shrimps (pandalus borealis). iii. indole and ph in shrimps caught in the barents sea compared with shrimps caught in the far east (author's transl)].the content of indole and the ph have been determined post mortem in shrimps (pandalus borealis) caught in the barents sea and in shrimps caught outside malaysia, india and taiwan. these two criteria were compared with organoleptic assessment and the contents of volatile nitrogen bases (ammonia, trimethylamine) and living bacteria. for shrimps caught in the barents sea, both raw shrimps stored in ice and processed (broiled, peeled and single-frozen) shrimps were investigated. the results showed ...19817329786
simultaneous determination of organotin compounds in fish and shellfish by gas chromatography with a flame photometric detector.in the present study the levels of the organotin compounds were determined in fish and shellfish which were purchased from retail markets in niigata, japan. the concentrations of dibutyltin (dbt), tributyltin (tbt), diphenyltin (dpt) and triphenyltin (tpt) compounds were separately determined by fpd-gas chromatography. eight species (yellowtail, tuna, cuttlefish, olive flounder, northern shrimp, cinnamon flounder, japanese sea bass and oyster) were studied and five samples of each species were a ...19938236246
cross-reactivity between ige-binding proteins from anisakis, german cockroach, and chironomids.anisakis simplex larvae parasitize animals used as seafood and can produce a specific immune response in man. the ingestion of seafood contaminated with stage three of a. simplex larvae can induce a specific ige response with clinical symptoms, usually urticaria, even if the fish is cooked before ingestion and the invasive infestation power destroyed by heating. our preliminary studies showed a strong association of a. simplex sensitization with ascaris lumbricoides, daphnia, chironomid spp., at ...19979201362
microbiological quality of icelandic cooked-peeled shrimp (pandalus borealis).iceland is a major producer of cold water shrimp, pandalus borealis. in recent years considerable attention has been given to improve hygiene in the factories producing cooked, peeled and frozen shrimp. to keep track of the bacteriological status of the end product, shrimp from most of the factories is routinely analysed bacteriologically by the request of shrimp exporters. this paper reports on the results of a bacteriological analysis of 7913 samples of shrimp from 26 icelandic factories over ...19989924947
cross-reactivity of some antibodies to human epitopes with shrimp pandalus borealis proteins: a possible aid in validation and characterization of crustacean cells in vitro.cell characterization of primary cultures in vertebrates is well established but not in marine invertebrates. this fact is hampering advances in the development of tissue cultures from this species. in the present study, a panel of antibodies to structural proteins, stress proteins, oncogenes and proliferation antigens, developed against mammalian antigens, were tested in paraffin sections of the crustacean pandalus borealis tissues. several tissues were analysed: hepatopancreas, gills, ovaries, ...200212125102
antibacterial activity in four marine crustacean decapods.a search for antibacterial activity in different body-parts of pandalus borealis (northern shrimp), pagurus bernhardus (hermit crab), hyas araneus (spider crab) and paralithodes camtschatica (king crab) was conducted. dried samples were extracted with 60% (v/v) acetonitrile, containing 0.1% (v/v) trifluoroacetic acid, and further extracted and concentrated on c18 cartridges. eluates from the solid phase extraction were tested for antibacterial, lysozyme and haemolytic activity. antibacterial act ...200212194450
molecular cloning and characterization of cathepsin b from the hepatopancreas of northern shrimp pandalus borealis.we cloned a cdna encoding cathepsin b from the hepatopancreas of northern shrimp pandalus borealis (nsctb). nucleotide sequence of the isolated clone encoded a preproenzyme of 328 amino acids, comprising a 15-residue putative signal peptide, a 60-residue propeptide and the 253-residue mature enzyme. the mature nsctb was 53% identical to human cathepsin b and conserved all the structural features characteristic of cysteine protease. the presence of an occluding loop in the mature region, a unique ...200312670793
heterologous expression in pichia pastoris and single-step purification of a cysteine proteinase from northern shrimp.a distinct cysteine proteinase (nscys) of northern shrimp pandalus borealis belonging to cathepsin l subgroup of the papain superfamily has been overexpressed as a precursor form (pronscys) in pichia pastoris. we adopted a simple and quick procedure to generate an expression cassette by constructing a donor vector harboring pronscys followed by recombination with an acceptor vector in a way so that the pronscys gene was placed downstream of the methanol-inducible aox1 promoter and alpha-mating f ...200314550639
shelf life and safety aspects of chilled cooked and peeled shrimps (pandalus borealis) in modified atmosphere packaging.to evaluate the growth of listeria monocytogenes and shelf life of cooked and peeled shrimps in modified atmosphere packaging (map).200515960666
biopreservation of brined shrimp (pandalus borealis) by bacteriocins from lactic acid bacteria.in brined shrimp (ca. 3% nacl), the effects of three different lactic acid bacteria bacteriocins (crude [6.54 x 10(sup10) u of bacteriocin activity {bu}/g] and purified [8.13 x 10(sup23) bu/g] nisin z, carnocin ui49 [2.32 x 10(sup4) bu/g], and crude bavaricin a [2.78 bu/g]) on bacterial growth and shelf life were compared with those of a benzoate-sorbate solution (0.1% each [wt/wt]) and a control with no preservatives. the shelf life of shrimp subjected to the control treatment was found to be 1 ...199516534936
contamination of cooked peeled shrimp (pandalus borealis) by listeria monocytogenes during processing at two processing plants.listeria spp. and listeria monocytogenes contamination was evaluated in cooked peeled shrimp (final or semifinal product, 82 samples) and the shrimp-processing environment (two plants, 613 samples) in eight surveys conducted from 1998 through 2001. listeria was detected in 12.5% (78) of the 695 samples (11.2% of the samples were positive for l. monocytogenes), but none of the samples of final product contained listeria. one hundred seventy-two l. monocytogenes isolates were characterized by puls ...200616786850
microbial changes and growth of listeria monocytogenes during chilled storage of brined shrimp (pandalus borealis).thirteen storage trials and ten challenge tests were carried out to examine microbial changes, spoilage and the potential growth of listeria monocytogenes in brined shrimp (pandalus borealis). shrimp in brine as well as brined and drained shrimp in modified atmosphere packaging (map) were produced and studied. different recipes were used to study the effect of preserving parameters (organic acids, ph and nacl) on growth of microorganisms and shelf life at 7-8 degrees c or 12 degrees c. particula ...200818456355
the enzyme and the cdna sequence of a thermolabile and double-strand specific dnase from northern shrimps (pandalus borealis).we have previously isolated a thermolabile nuclease specific for double-stranded dna from industrial processing water of northern shrimps (pandalus borealis) and developed an application of the enzyme in removal of contaminating dna in pcr-related technologies.201020421970
synthesis of the red-pigment-concentrating hormone of the shrimp, pandalus borealis. 20154433570
cuticular proteins from the shrimp, pandalus borealis.1. the percentages of mineral salts, chitin, and urea-extractable and non-extractable proteins were determined in pieces of cuticle from selected body regions of the shrimp, pandalus borealis. 2. two-dimensional gel-electrophoresis of the urea-extractable proteins shows that a large number of different proteins are present. identical protein patterns are obtained from the various cuticular regions. 3. a fractionation scheme is presented, which is suitable for obtaining the major proteins in quan ...20061764926
an efficient multistrategy dna decontamination procedure of pcr reagents for hypersensitive pcr applications.pcr amplification of minute quantities of degraded dna for ancient dna research, forensic analyses, wildlife studies and ultrasensitive diagnostics is often hampered by contamination problems. the extent of these problems is inversely related to dna concentration and target fragment size and concern (i) sample contamination, (ii) laboratory surface contamination, (iii) carry-over contamination, and (iv) contamination of reagents.201020927390
mapping of the immunodominant regions of shrimp tropomyosin pan b 1 by human ige-binding and ige receptor crosslinking studies.epitope mapping of an allergen is generally done by ige-binding assays with short synthetic peptides, but this provides little information about which domains are responsible for ige receptor crosslinking on effector cells. our aim was to map the immunodominant regions of shrimp tropomyosin by both ige-binding and ige-receptor crosslinking studies.201323817275
structural and immunological characterization of recombinant pan b 1, a major allergen of northern shrimp, pandalus borealis.shellfish allergy is one of the major causes of life-threatening allergic reactions to food. the shrimp species pandalus borealis is the commercially most important coldwater shrimp species, and its protein extract is commonly used in shrimp allergy diagnostics. however, the dna sequence of its major allergen, tropomyosin, designated pan b 1, was not previously described. our aim was to identify the cdna sequence of pan b 1 and to generate a recombinant protein with similar structure and allerge ...201323075760
effect of brine marination on survival and growth of spoilage and pathogenic bacteria during processing and subsequent storage of ready-to-eat shrimp (pandalus borealis).the effect of brine marination at chill temperatures on survival and growth of spoilage and pathogenic bacteria during processing and subsequent storage of ready-to-eat cold water shrimp was studied. survival and growth of lactobacillus sakei, listeria monocytogenes, salmonella, staphylococcus aureus and vibrio parahaemolyticus were examined. the effect of brine composition and ph was determined in 12 screening experiments without addition of shrimp. sixteen challenge tests with shrimp were then ...201222564452
[cloning and expression analysis of cathepsin l cdna of exopalaemon carinicauda].based on the est sequence from a hemocyte cdna library, the cathepsin l cdna of exopalaemon carinicauda (eccatl) was cloned by rapid amplification of cdna ends (race). the eccatl cdna was 1136 bp in length, which contains an open reading frame (orf) of 960 bp, encoding a 319 amino-acid polypeptide. homology analysis revealed that the amino acid sequence of eccatl was highly conserved with its homologs in other crustaceans. the similarities of eccatl with the catl of palaemonetes varians and pand ...201323389977
[characteristics of proteinase digestive function in invertebrates--inhabitants of cold seas].digestive proteinases of various taxa of invertebrates of the northern seas have been studied: crustaceans paralithodes camtchaticus, pandalus borealis; molluscs chlamys islandicus, buccinum undatum, serripes groenlandicus, and echinoderms strongylocentrotus droebachiensis, cucumaria frondosa, asterias rubens, and grossaster papposus. the presence of two proteolytic activity peaks in the acid (ph 2.5-3.5) and low alkaline zones (ph 7.5-8.5) and a similar proteinase spectrum have been revealed in ...201218038635
isolation and characterization of the novel lipophilic protein, pb cp-12.7, from the shell of the pink shrimp, pandalus borealis.past research on diapause-inducing substances of the silkworm has isolated an extremely lipophilic peptide and demonstrated its unique characteristics. in the present work, similar lipophilic proteins were searched for in the shell of the shrimp, pandalus borealis, and one novel protein, pb cp-12.7, was isolated. its structure comprising 126 amino acids was revealed by a combination of a sequence analysis and the enzymic fragmentation technique. pb cp-12.7 is unique in that it was insoluble in n ...200111440115
modelling gastric evacuation in gadoids feeding on crustaceans.a mechanistic, prey surface-dependent model was expanded to describe the course and rate of gastric evacuation in predatory fishes feeding on crustacean prey with robust exoskeletons. this was accomplished by adding a layer of higher resistance to the digestive processes outside the inner softer parts of a prey cylinder abstraction and splitting up the prey evacuation into two stages: an initial stage where the exoskeleton is cracked and a second where the prey remains are digested and evacuated ...201627170110
prey exoskeletons influence the course of gastric evacuation in atlantic cod gadus morhua.this study examined the effects of prey exoskeleton characteristics on gastric evacuation patterns in atlantic cod gadus morhua. three distinct stages were highlighted in the gastric evacuation of crustacean prey characterized by a robust exoskeleton. the experiments confirmed that the three shrimp species, pandalus borealis, pandalus montagui and eualus macilentus, and the crab chionoecetes opilio, were evacuated from the stomach at different rates. the duration of all stages increased with inc ...201323464544
snow crab allergy and asthma among greenlandic workers--a pilot study.to study snow crab sensitization, occupational allergy and asthma in the snow crab industry in greenland, as high rates have been found in canada, but no reports have emerged from the same industry in greenland.201222901288
cross-reactivity of ige-binding components between boiled atlantic shrimp and german cockroach.ige-antibody reactivity to boiled atlantic shrimp (pandalus borealis) and german cockroach (blattella germanica) of sera from 89 patients, sensitive to one or the other, was investigated with an enzymatic immunoassay for specific ige detection (cap-feia system, pharmacia, sweden). ige serum levels to both antishrimp and anticockroach allergens were found to be positive in 76 of the 89 (85.4%) tested sera. a positive anticockroach ige was very rare in the absence of detectable antishrimp ige (fiv ...19958748725
secretagogues and growth factors in fish and crustacean protein hydrolysates.: the search for new molecules in fish protein hydrolysates is of great interest in animal feeding as it is in aquaculture, fertilizer, cosmetic, and pharmacologic domains. different sources of hydrolysates such as shrimp waste (pandalus borealis), cod (gadus morhua) head, and head and viscera of sardine (sardina pilchardus), obtained after hydrolysis or autolysis, were tested on fibroblast cell cultures and by gastrin radioimmunoassay. the level of hydrolysis seems to play an important role in ...199910525683
chemical fractionation of shrimp extracts inducing bottom food search behavior in cod (gadus morhua l.).the bottom food search (bfs) feeding behavior in cod (gadus morhua l.), has been used in a bioassay for chemical isolation of the feeding stimulant substances present in shrimp (pandalus borealis). an aqueous methanol extract of ground shrimp was separated into acidic, neutral, and amphoteric/basic fractions by ion-exchange chromatography and into single components by preparative high-pressure liquid chromatography (hplc). of the isolated single components, the amino acid glycine was most potent ...198624306405
an unusual and heavy infection of a prawn, pandalus borealis kroyer, by a nematode, contracaecum sp. 195413212539
changes in antioxidative capacity of saithe (pollachius virens) and shrimp (pandalus borealis) during in vitro digestion.the health effects of seafood have primarily been linked to eicosapentaenoic acid (epa; 20:5n-3) and docosahexaenoic acid (dha; 22:6n-3). these omega-3 fatty acids are highly susceptible to oxidation. peptides exhibiting antioxidative capacity (aoc) are encrypted in seafood muscle proteins. such components may protect the fatty acids during digestion and uptake, as well as exhibit antioxidative effects in general. the oxygen radical absorbance capacity (orac) assay and the ferric reducing antiox ...200919845349
biological activity of the predicted red pigment-concentrating hormone of daphnia pulex in a crustacean and an insect.the elucidation of the genome of the waterflea daphnia pulex made it possible to search for orthologue genes for the crustacean red pigment-concentrating hormone (named panbo-rpch after the species pandalus borealis in which the red pigment-concentrating hormone was first identified); panbo-rpch is a member of the adipokinetic hormone (akh)/red pigment-concentrating hormone (rpch) peptide family. the information pointed to a putative mature rpch octapeptide in d. pulex with the primary sequence ...201019686751
the organization of the lamina ganglionaris of the prawn, pandalus borealis (kröyer).the lamina ganglionaris (first optic neuropile) of the decapod crustacean pandalus borealis has its optic cartridges (synaptic compartments) arranged in horizontal rows. each optic cartridge contains seven receptor axon terminals and the branching axis fibres of five monopolar second order neurons. four types of monopolar neurons are classified. their cell bodies are arranged in two layers. the inner layer contains the cell bodies of exclusively one of these types, and each cartridge is invaded ...20051201587
seasonal changes in migratory and predatory activity of two species of gadoid preying on inshore northern shrimp pandalus borealis.the interaction between two species of gadoid and a shrimp stock was studied in a 40 km long two-armed fjord in north-west iceland. on the basis of acoustic and trawl surveys in 2005 and 2006, immature cod gadus morhua and haddock melanogrammus aeglefinus were found to migrate to the inner part of the fjord in late summer, concurrent with rising temperatures. at the same time, the local northern shrimp pandalus borealis stock retreated into the north-east arm of the fjord. vertical distribution ...201121463310
marine species in ambient low-oxygen regions subject to double jeopardy impacts of climate change.we have learned much about the impacts of warming on the productivity and distribution of marine organisms, but less about the impact of warming combined with other environmental stressors, including oxygen depletion. also, the combined impact of multiple environmental stressors requires evaluation at the scales most relevant to resource managers. we use the gulf of st. lawrence, canada, characterized by a large permanently hypoxic zone, as a case study. species distribution models were used to ...201627753179
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