| numerical taxonomy of coryneform bacteria isolated from pond-reared shrimp (penaeus aztecus) and pond water. | gram-positive, catalase-positive, nonsporeforming, pleomorphic rods isolated from pond-reared shrimp and pond water were compared with type cultures of the corynebacteriaceae. classification of the type cultures based on 66 cell and colony characters proved comparable to one based on 163 morphological, biochemical, and physiological characters. this similarity was not observed with pond isolates. with the aid of numerical analysis, pond isolates could be placed into six major groups based on cer ... | 1972 | 4551043 |
| identification of the major brown shrimp (penaeus aztecus) allergen as the muscle protein tropomyosin. | shrimp, a major seafood allergen, was investigated as a model food allergen. extracts from both shrimp (penaeus aztecus) meat and cooking fluid contain a substantial and similar amount of allergenic activity. a 36-kd allergen, demonstrated in both extracts by sds-page/western blot analysis, reacted with 28/34 (82%) sera from shrimp-sensitive, skin test and rast-positive, individuals. this allergen, named pen a i, was isolated by sds-page; its amino acid composition was rich in aspartic and gluta ... | 1994 | 7916224 |
| susceptibility to taura syndrome virus of some penaeid shrimp species native to the gulf of mexico and the southeastern united states | experimental studies demonstrated that penaeus setiferus, but not penaeus aztecus or penaeus duorarum, could be killed by taura syndrome virus (tsv). however, specimens of p. setiferus that survived infection and both p. aztecus and p. duorarum at least 79 days postexposure that did not demonstrate gross signs of infection were shown to harbor virus by bioassay using penaeus vannamei, a highly susceptible target host. consequently, all three of those penaeids native to the southeast united state ... | 1997 | 9056467 |
| ige and monoclonal antibody reactivities to the major shrimp allergen pen a 1 (tropomyosin) and vertebrate tropomyosins. | pen a 1, the major shrimp allergen from brown shrimp penaeus aztecus has been identified as the muscle protein tropomyosin. to identify pen a 1 ige binding sites, the reactivities of pen a 1-specific monoclonal antibodies (mabs) and shrimp-allergic subjects' ige to shrimp and homologous mammalian tropomyosins were analyzed. pen a 1, purified by preparative sds-page and commercially obtained porcine, bovine and rabbit tropomyosin were cleaved by cnbr or digested by endoproteinases lys-c, glu-c, t ... | 1996 | 9095246 |
| characterization of recombinant shrimp allergen pen a 1 (tropomyosin). | tropomyosin (pen a 1) from brown shrimp, penaeus aztecus, has been identified as the only major shrimp allergen. since beef, pork and chicken are other tropomyosin-containing foods that are not very allergenic, tropomyosins can serve to investigate the contribution of the structural properties of a protein to its allergenicity. the aim of this study was to determine the primary structure of pen a 1 and to identify ige-binding epitopes. the screening of a unidirectional expression cdna library fr ... | 1997 | 9130534 |
| purification, characterization, and hydrodynamic properties of arginine kinase from gulf shrimp (penaeus aztecus). | arginine kinase from the tail muscle of the gulf shrimp (penaeus aztecus) was purified to apparent homogeneity, using a rapid, high-yield method. the protein exhibits a molecular weight of 40 kda according to the methods of gel filtration and gel electrophoresis in sodium dodecyl sulfate, also indicating that arginine kinase from shrimp is a monomer. the amino acid content of arginine kinase from shrimp is similar to arginine kinases from several species and to creatine kinase from rabbit muscle ... | 1997 | 9281313 |
| b- and t-cell epitopes of tropomyosin, the major shrimp allergen. | the major crustacean allergen characterized from different species of shrimp is the muscle protein tropomyosin. two shared epitopes corresponding to 47-63 and 150-158 of the deduced amino-acid sequence of the brown shrimp, m. ensis, were identified as ige-binding b-cell epitopes. a 21-mer peptide spanning the amino-acid residues 261-281 was identified as a putative t-cell epitope capable of reducing ongoing tropomyosin-specific igg and ige responses in a mouse model. these observations suggest t ... | 1998 | 9825997 |
| effects of hypoxia on movements and behavior of selected estuarine organisms from the southeastern united states. | hypoxia, or low dissolved oxygen, remains a common occurrence in estuarine waters as human activity in coastal areas expands. fish kills, probably the most recognized indicator of these and other water quality problems, have significantly increased in recent years in many southeastern united states estuaries. while entire aquatic communities are impacted by changes in available oxygen, estuarine organisms serve as appropriate indicators of these changes as they exhibit complex physiological and ... | 2000 | 10841932 |
| effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp. | this study was performed to evaluate the application of food irradiation technology as a method for reducing shrimp allergy without adverse effects. shrimp heat-stable protein (hsp) was isolated and gamma irradiated at 0, 1, 3, 5, 7, or 10 kgy in the condition of solution (1 mg/ml), and fresh shrimp was also irradiated. conformational change of irradiated hsp was monitored by means of spectrometric measures, enzyme-linked immunosorbent assay with mouse monoclonal antibody, or human patients' ser ... | 2000 | 10914665 |
| isolation of vibrio and pseudomonas from brown shrimp (penaeus californiensis holmes) intestine. | bacteria of the genera vibrio, pseudomonas and aeromonas were isolated from the intestine of apparently healthy brown shrimp (penaeus californiensis holmes) cultured in a tidal pond. species from these genera of bacteria have been reported as shrimp pathogens and have been involved in human gastrointestinal disorders related to seafood consumption. isolation was done first in marine broth, then in selective media (tcbs, cetrimide and macconkey). the oxidase negative strains were discarded as ins ... | 1997 | 10932719 |
| is the brown shrimp crangon crangon (l.) population of the vaccarès lagoon (camargue, france, rhône delta) an annual population? | to study the population dynamics of crangon crangon in the vaccarès lagoon in the camargue (rhône delta), the age of brown shrimp was determined using the method of tiews (1954). this method consists in counting the number of segments of the outer branch of the first antenna. this number is correlated with the number of moults already accomplished by the shrimp. the duration of the intermoult depends on water temperature. adapting this model to our environmental data, we determined the age of th ... | 2000 | 11019369 |
| secretagogue activities in cod (gadus morhua) and shrimp (penaeus aztecus) extracts and alcalase hydrolysates determined in ar4-2j pancreatic tumour cells. | peptides with gastrin immunoreactivity were measured in cod muscle (gadus morhua) and shrimp heads (penaeus aztecus) extracts and alcalase hydrolysates and separated by two chromatographic steps. secretagogue activities present in crude extracts fractions were examined with or without specific antagonists of cck receptors in ar4-2j cells. several sub-fractions significantly stimulate amylase release, up to 110%. these stimulatory effects could be completely inhibited by the presence of l 365, 26 ... | 2003 | 12670792 |
| pathology and ultrastructure of an intranuclear bacilliform virus (ibv) infecting brown shrimp crangon crangon (decapoda: crangonidae). | the brown shrimp crangon crangon supports an important fishery in europe (over 25000 t, valued at 80 million euros in 2000). through the course of histopathological screening of crustaceans from the clyde estuary, western scotland, for the biological effect of contaminants, we have discovered a highly prevalent (up to 100%) non-occluded intranuclear bacilliform virus (ibv) infection in the hepatopancreatic tubule epithelia and midgut epithelia of wild c. crangon. this is the first report of an i ... | 2004 | 15109130 |
| sampling and evaluation of white spot syndrome virus in commercially important atlantic penaeid shrimp stocks. | in 1997, white spot syndrome virus (wssv) was discovered in shrimp culture facilities in south carolina, usa. this disease was known to cause devastating mortalities in cultured populations in southeast asia and prompted concern for the health of wild populations in the usa. our study surveyed wild shrimp populations for the presence of wssv by utilizing molecular diagnostics and bioassay techniques. a total of 1150 individuals (586 litopenaeus setiferus, 477 farfantepenaeus aztecus and 87 f. do ... | 2004 | 15264713 |
| recombinant tropomyosin from penaeus aztecus (rpen a 1) for measurement of specific immunoglobulin e antibodies relevant in food allergy to crustaceans and other invertebrates. | immunoglobulin e (ige)-mediated food allergy to crustaceans and mollusks is relatively common and affected individuals typically react to a range of different species. the only known major allergen of shrimp was first described over 20 years ago and later identified as the muscle protein tropomyosin. this protein may be useful as a defined and relevant diagnostic marker for allergic sensitization to invertebrate foods. in order to generate an assay reagent suitable for this purpose, tropomyosin ... | 2004 | 15672477 |
| microbial flora of pond-reared brown shrimp (penaeus aztecus). | agar plate counts and microbial types are reported for brown shrimp reared in 2-acre natural marshland and in 0.5-acre artificial ponds during june to october 1970. bacterial counts of pond-reared shrimp ranged from 5 x 10 to 5.5 x 10 per g. at final harvest in october, bacterial counts ranged from 2 x 10 to 5.5 x 10 per g. in marsh ponds, bacterial counts of shrimp and pond water were lowest in august when both water temperature and salinity were high. coryneform bacteria and to a lesser extent ... | 1971 | 16349908 |
| cloning and characterisation of mitochondrial manganese superoxide dismutase (mtmnsod) from the giant freshwater prawn macrobrachium rosenbergii. | a cdna encoding a mitochondrial manganese superoxide dismutase (mtmnsod) was cloned from the hepatopancreas of giant freshwater prawn macrobrachium rosenbergii using reverse transcription polymerase chain reaction (rt-pcr) by degenerate primers. both 3'- and 5'-regions were isolated by rapid amplification of cdna end (race) pcr method. analysis of nucleotide sequence revealed that the mtmnsod full-length cdna consists of 1202bp containing an open reading frame of 654bp, which encodes a protein c ... | 2006 | 16621606 |
| structural, immunological and functional properties of natural recombinant pen a 1, the major allergen of brown shrimp, penaeus aztecus. | recombinant allergens are considered the basis for new diagnostic approaches and development of novel strategies of allergen-specific immunotherapy. as pen a 1 from brown shrimp penaeus aztecus is the only major allergen of shrimp and binds up to 75% of all shrimp-specific ige antibodies this molecule may be an excellent model for the usage of allergens with reduced ige antibody-binding capacity for specific immunotherapy. | 2006 | 16630158 |
| allergen motifs and the prediction of allergenicity. | we have recently shown that the majority of allergens can be represented by allergen motifs. this observation prompted us to experimentally investigate the synthesized peptides corresponding to the in silico motifs with regard to potential ige binding and cross-reactions with allergens. two motifs were selected as examples to conduct in vitro studies. from the first motif, derived from allergenic mnsod sequences, the motif stretch of the allergen asp f 6 was selected and synthesized as a peptide ... | 2007 | 17303251 |
| reactivity of shrimp allergy-related ige antibodies to krill tropomyosin. | krill, which morphologically resembles small shrimp, represents small ocean crustaceans and has been used for human consumption in japan and some other countries. the major allergen in crustaceans has been reported to be tropomyosin, but the allergenicity of krill tropomyosin remains uncertain. | 2008 | 17912005 |
| bioaccumulation of trace metals in the brown shrimp crangon crangon (linnaeus, 1758) from the german wadden sea. | the objective of the present study is to evaluate the suitability of the brown shrimp crangon crangon (linnaeus, 1758) from the german wadden sea as a biomonitor for the trace metals cd, pb, cu, and zn and to analyse whether the two-compartment model sensu oecd could be used as a predictive tool to assess environmental quality. the tested decapods accumulated cd and pb upon exposure and it was possible to estimate significant model parameters of two-compartment models, while they did not respond ... | 2008 | 18571744 |
| inhibition of multidrug/xenobiotic resistance transporter by mk571 improves dye (fura 2) accumulation in crustacean tissues from lobster, shrimp, and isopod. | multidrug/xenobiotic resistance transporters are present in living organisms as a first line defence system against small, potentially harmful molecules from the environment or from internal metabolic reactions. multidrug resistance associated proteins (mrp) are one type of atp-binding-cassette (abc) transporters, which also transport dyes such as fura 2, a calcium chelating fluorescence indicator. the specific mrp inhibitor mk571 was used to investigate the fluorescence intensity of cells in ti ... | 2009 | 19501673 |
| nuclear dna content variation associated with muscle fiber hypertrophic growth in decapod crustaceans. | we tested the hypothesis that hypertrophic muscle growth in decapod crustaceans is associated with increases in both the number of nuclei per fiber and nuclear dna content. the dna-localizing fluorochrome dapi (4',6-diamidino-2-phenylindole) and chicken erythrocyte standards were used with static microspectrophotometry and image analysis to estimate nuclear dna content in hemocytes and muscle fibers from eight decapod crustacean species: farfantepenaeus aztecus, palaemonetes pugio, panulirus arg ... | 2010 | 20237594 |
| the invasion of five alien species in the delta do parnaíba environmental protection area, northeastern brazil. | marine biological invasions have been regarded as one of the major causes of native biodiversity loss, with shipping and aquaculture being the leading contributors for the introductions of alien species in aquatic ecosystems. in the present study, five aquatic alien species (one mollusk, three crustaceans and one fish species) were detected during dives, shore searches and from the fisheries on the coast of the delta do parnaíba environmental protection area, in the states of piauí and maranhão, ... | 2010 | 20737846 |
| Organotins in North Sea brown shrimp (Crangon crangon L.) after implementation of the TBT ban. | The organotin (OT) compounds tributyltin (TBT) and triphenyltin (TPhT) are potent biocides that have been used ubiquitously in antifouling paints and pesticides since the mid-1970s. These biocides are extremely toxic to marine life, particularly marine gastropod populations. The European Union therefore took measures to reduce the use of TBT-based antifouling paints on ships and ultimately banned these paints in 2003. Despite sufficient data on OT concentrations in marine gastropods, data are sc ... | 2011 | 22154339 |
| ultrastructural observations of the muscle insertion and modified branchiostegite epidermis in the larval brown shrimp, penaeus aztecus. | | 2015 | 4652146 |
| high level expression, purification and physico- and immunochemical characterisation of recombinant pen a 1: a major allergen of shrimp. | well-characterised and immunologically active recombinant allergens are of eminent importance for improvement of diagnostic tools and immunotherapy of allergic diseases. the use of recombinant allergens has several advantages such as the more precise quantification of the active substance compared to allergen extracts and the reduced risk of contamination with other allergenic proteins compared to purified natural allergens. optimised standard protocols for expression and purification and a deta ... | 2008 | 18727010 |
| volatile compounds associated with psychrobacter spp. and pseudoalteromonas spp., the dominant microbiota of brown shrimp (crangon crangon) during aerobic storage. | the spoilage potential of several psychrobacter and pseudoalteromonas species (psychrobacter cibarius, psychrobacter maritimus, pseudoalteromonas elyakovii, pseudoalteromonas paragorgicola and pseudoalteromonas nigrifaciens) was determined and quantified based on the presence of volatile organic compounds (vocs). psychrobacter and pseudoalteromonas species dominate the microbiota of cooked brown shrimp (crangon crangon). additionally, api zym analyses determined the species' enzymatic capacity t ... | 2013 | 24042000 |
| molecular identification of the microbiota of peeled and unpeeled brown shrimp (crangon crangon) during storage on ice and at 7.5 °c. | the dominant microbiota of brown shrimp (crangon crangon) were systematically identified during storage under different conditions. freshly caught shrimp were processed on board the fishing vessel under the best possible hygienic conditions (ideal), unpeeled and manually (sterile) peeled, then stored on ice and at 7.5 °c until microbiologically spoiled. results were compared with industrially processed (industrial) shrimp. isolates grown on various media were identified by 16s rrna and gyrb gene ... | 2013 | 24010590 |
| paradoxium irvingi n.gen. n.sp. (microsporidia) infecting the musculature of european pink shrimp pandalus montagui. | this paper utilises histological, ultrastructure and molecular phylogenetic data to describe a novel genus and species (paradoxium irvingi n.gen., n.sp.) within clade 5 of the phylum microsporidia. the parasite infects the musculature of the pink shrimp pandalus montagui captured from united kingdom waters. the novel microsporidium is morphologically and phylogenetically dissimilar to its nearest phylogenetic branch relative thelohania butleri infecting the sister shrimp taxon pandalus jordani. ... | 2015 | 26146229 |
| turbidity interferes with foraging success of visual but not chemosensory predators. | predation can significantly affect prey populations and communities, but predator effects can be attenuated when abiotic conditions interfere with foraging activities. in estuarine communities, turbidity can affect species richness and abundance and is changing in many areas because of coastal development. many fish species are less efficient foragers in turbid waters, and previous research revealed that in elevated turbidity, fish are less abundant whereas crabs and shrimp are more abundant. we ... | 2015 | 26401444 |
| absence of ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in c. crangon and p. serratus. | mitochondria from the embryos of brine shrimp (artemia franciscana) do not undergo ca(2+)-induced permeability transition in the presence of a profound ca(2+) uptake capacity. furthermore, this crustacean is the only organism known to exhibit bongkrekate-insensitive mitochondrial adenine nucleotide exchange, prompting the conjecture that refractoriness to bongkrekate and absence of ca(2+)-induced permeability transition are somehow related phenomena. here we report that mitochondria isolated fro ... | 2012 | 22768139 |
| trace elements and organochlorines in the shoalgrass community of the lower laguna madre, texas. | our objectives were to measure concentrations of seven trace elements and 14 organochlorine compounds in sediment and biota of the shoalgrass (halodule wrightii) community of the lower laguna madre of south texas and to determine whether chemicals associated with agriculture (e.g. mercury, arsenic, selenium, organochlorine pesticides) were highest near agricultural drainages. arsenic, mercury, selenium, lead, cadmium, and organochlorines were generally at background concentrations throughout the ... | 1993 | 24225804 |
| corrigendum to “persistent organic pollutants in the scheldt estuary: environmental distribution and bioaccumulation[environ. int. 48c (2012) 17–27]. | levels of polychlorinated biphenyls (pcbs), polybrominated diphenyl ethers (pbdes) and organochlorine pesticides (ocps) were determined in the sediment and in several species (european flounder,platichthys flesus; common sole, solea solea; chinese mitten crab,eriocheir sinensis; shore crab, carcinus maenas; brown shrimp,crangon crangon; blue mussel, mytilus edulis and bristle worms,polychaeta) from 7 locations in the scheldt estuary (se, the netherlands–belgium). overall pop levels in the sedime ... | 2014 | 24505601 |
| hematodinium sp. and its bacteria-like endosymbiont in european brown shrimp (crangon crangon). | parasitic dinoflagellates of the genus hematodinium are significant pathogens affecting the global decapod crustacean fishery. despite this, considerable knowledge gaps exist regarding the life history of the pathogen in vivo, and the role of free living life stages in transmission to naïve hosts. | 2012 | 22958655 |
| persistent organic pollutants in the scheldt estuary: environmental distribution and bioaccumulation. | levels of polychlorinated biphenyls (pcbs), polybrominated diphenyl ethers (pbdes) and organochlorine pesticides (ocps) were determined in the sediment and several species (european flounder, platichthys flesus; common sole, solea solea; chinese mitten crab, eriocheir sinensis; shore crab, carcinus maenas; brown shrimp, crangon crangon; blue mussel, mytilus edulis and bristle worms, polychaeta) from 7 locations in the scheldt estuary (se, the netherlands-belgium). overall pop levels in the sedim ... | 2012 | 22832186 |
| a histopathological survey of shore crab (carcinus maenas) and brown shrimp (crangon crangon) from six estuaries in the united kingdom. | invertebrates show considerable potential as sentinel organisms for the monitoring of the health status of aquatic systems. they are generally small, abundant, relatively sessile, and may readily bioaccumulate toxins. cascade-like stress responses can occur following acute or chronic exposures to contaminated environments and as such, the overall health status of individuals within those environments, both in terms of histopathological lesions and the presence of infecting organisms, may ultimat ... | 2005 | 15766930 |
| sem characterization of anatomical variation in chitin organization in insect and arthropod cuticles. | the cuticles of insects and arthropods have some of the most diverse material properties observed in nature, so much so that it is difficult to imagine that all cutciles are primarily composed of the same two materials: a fibrous chitin network and a matrix composed of cuticle proteins. various factors contribute to the mechanical and optical properties of an insect or arthropod cuticle including the thickness and composition. in this paper, we also identified another factor that may contribute ... | 2016 | 26774746 |
| escape trajectories of the brown shrimp crangon crangon, and a theoretical consideration of initial escape angles from predators | tail-flip escape trajectories of the brown shrimp crangon crangon have been investigated in response to a natural predator, the cod gadus morhua, and an artificial stimulus. shrimps escaped by rolling to their left or right during the initial tail-flip of a response, and thereafter swam on their side. as a result of the laterally directed first tail-flip, initial escape angles always lay between 75 degrees and 156 degrees with respect to the (pre-escape) longitudinal axis (anterior=0 degrees) of ... | 1999 | 9851908 |
| pcbs in cod (gadus morhua), flounder (platichthys flesus), blue mussel (mytilus edulis) and brown shrimp (crangon crangon) from the belgian continental shelf: relation to biological parameters and trend analysis. | pcb levels in cod, flounder, mussel and shrimp, covering a ten-year period, were assessed for temporal trends and their relation to biological parameters. a significant relation was found between the pcb levels on a wet weight basis and the total lipid content. normalising on the total lipid content reduced the differences in pcb levels between the organisms and between different tissues within the organisms. a general downward trend was observed for the pcb levels on the belgian continental she ... | 2013 | 9828337 |
| prevalence of viral pathogens wssv and ihhnv in wild organisms at the pacific coast of mexico. | this study investigated whether white spot syndrome virus and infectious hypodermal and hematopoietic necrosis virus, can survive in wild invertebrates and vertebrates in the environment surrounding shrimp farms along the pacific coast of mexico. the evidences imply that both viruses have a potential of persisting in crabs, blue, white and brown shrimps. the most prevalent virus, ihhnv was present in 19.5% (344/1736) followed by wssv in 3.6% (65/1736). coinfection of wssv and ihhnv was also dete ... | 2014 | 24300441 |
| photostimulated luminescence detection of irradiated shellfish: international interlaboratory trial. | an interlaboratory trial was conducted to validate photostimulated luminescence (psl) detection of irradiated shellfish. five species of shellfish (nephrops norvegicus, mussels, black tiger prawns, brown shrimps, and king scallops) were presented blind as nonirradiated and irradiated to 0.5 and 2.5 kgy. precharacterization analysis of each product and treatment was performed on both whole (including shell) and intestinal samples. the results for whole samples (including shell) confirmed that the ... | 2008 | 14632401 |
| thermoluminescence detection of irradiated shellfish: international interlaboratory trial. | an international interlaboratory trial was conducted using thermoluminescence for the detection of irradiated shellfish, aimed at validating the method for routine use. nephrops norvegicus, mussels, brown shrimps, black tiger prawns, and king scallops were presented as nonirradiated and irradiated to 0.5 and 2.5 kgy. the protocol called for the use of 3 preparation methods: extraction of silicates from whole shellfish by acid hydrolysis and physical separation, and of carbonates from powdered sh ... | 2008 | 14632400 |
| spoilage potential of vagococcus salmoninarum in preservative-free, map-stored brown shrimp and differentiation from brochothrix thermosphacta on streptomycin thallous acetate actidione agar. | during a previous study concerning brown shrimp (crangon crangon), selective streptomycin thallous acetate actidione (staa) agar was used to determine the growth of brochothrix thermosphacta. however, the growth of vagococcus salmoninarum on this medium was also noticed. this study explores the spoilage potential of this organism when inoculated on sterile shrimp. | 2016 | 26913473 |