| vargula hilgendorfii luciferase: a secreted reporter enzyme for monitoring gene expression in mammalian cells. | the small marine ostracod crustacean, vargula hilgendorfii, produces a bright blue luminous secretion which is ejected into seawater. the luminescence is due to a simple enzyme-catalyzed reaction involving only luciferase, luciferin (substrate), and molecular oxygen. thus, v. hilgendorfii luciferase (vl) should be useful as a reporter enzyme in studies of gene expression in mammalian cells. expression plasmids consisting of vl cdna (vl) linked to the promoters simian virus 40 early region, rous ... | 1990 | 2269435 |
| expression of a bifunctional chimeric protein a-vargula hilgendorfii luciferase in mammalian cells. | we have designed and constructed a novel chimeric protein that consisted of a single domain of protein a and luciferase derived from sea-firefly vargula hilgendorfii with the goal of obtaining a heterofunctional immunological tool. the structural gene of luciferase was fused to the 3' terminus of the d domain gene of protein a with/without a short linker of five amino acids. the resulting constructs under the transcriptional regulation of the rous sarcoma virus (rsv) promoter, were expressed tra ... | 1996 | 8770415 |
| continuous measurement of targeted promoter activity by a secreted bioluminescence reporter, vargula hilgendorfii luciferase. | the promoter activity of growth hormone (gh) was continuously monitored in rat pituitary adenoma cells (gh3) by a secreted bioluminescence reporter, vargula hilgendorfii (vh) luciferase. the sensitivity of the reporter was approximately 60-times higher than that of the firefly luciferase. gh3 cells were transfected with a plasmid containing a dna sequence of the gh promoter (1.8 kb) and a full length of the vh luciferase cdna. using the stable transformants, the vh luciferase activity was monito ... | 2001 | 11161320 |
| [development of a new method for sequential monitoring of transcriptional activities of growth hormone genes by a secreted bioluminescence reporter]. | the genetic reporter system has been introduced as a tool for sensitive and convenient monitoring of gene expression. in the present study, the promoter activity of growth hormone (gh)-coding gene was sequentially monitored in gh3 cells (rat pituitary adenoma cell-line) by means of bioluminescence which was evoked by a secreted reporter, vargula hilgendorfii (vh) luciferase. three kinds of expression plasmids were constructed, which consisted of a rat gh promoter fragment and vh luciferase cdna, ... | 2000 | 11193933 |
| differential expression of duplicated opsin genes in two eyetypes of ostracod crustaceans. | in the first molecular study of ostracod (crustacea) vision, we present partial cdna sequences of ostracod visual pigment genes (opsins). we found strong support for differential expression of opsins in ostracod median and compound eyes and suggest that photoreceptor specific expression may be a general phenomenon in organisms with multiple receptors. we infer that eye-specific expression predates the divergence of the two species examined, skogsbergia lerneri and vargula hilgendorfii, because e ... | 2004 | 15486697 |
| biogeography of luminous marine ostracod driven irreversibly by the japan current. | the biogeography of the luminous marine ostracod vargula hilgendorfii, also called "umihotaru," shows that this organism may have arrived relatively recently on the japanese islands during the final glacier period approximately 10,000 years ago. phylogenetic relationships also strongly indicate that the japan current drove the umihotaru ostracod northward. it is evident that the umihotaru ostracod spread rapidly to the major japanese islands 3,000 km north, whereas its spread was slow in the sou ... | 2005 | 15858206 |
| single cell reporter assay using cell surface displayed vargula luciferase. | reporter genes such as firefly luciferase are common tools to monitor gene expression in various systems. as reporter gene represents the expression level of the gene of interest with its enzyme activity, firefly luciferase is most frequently used because its luminescent activity is highly sensitive and less time consumable for assay. however, since firefly luciferase is expressed internally in the cell, lysis of the cell is a critical step, and thus it is difficult to monitor the gene expressio ... | 2001 | 16233149 |
| new reporter system for per1 and bmal1 expressions revealed self-sustained circadian rhythms in peripheral tissues. | a new reporter system for monitoring expressions of two clock genes, per1 and bmal1, from a single tissue in culture was developed in mice. reporters are vargula hilgendorfii luciferase (vl) and firefly luciferase (fl), whose activities are increased in parallel with per1 and bmal1 expressions, respectively. formal properties of the circadian system in transgenic mice are indistinguishable from those in wild-type animals. circadian rhythms in per1-vl and bmal1-fl in the suprachiasmatic nucleus ( ... | 2006 | 16999737 |
| concerted evolution of duplicated control regions within an ostracod mitochondrial genome. | the luminescent marine ostracod vargula hilgendorfii comprises distinct populations around the japanese islands. its mitochondrial dna is unusual, with duplicated control regions (crs; cr#1 and cr#2). we determined the sequences of ostracod crs in 7 different populations. the sequences of cr#1 and cr#2 within any population were extremely similar, above 99.7%; moreover, their derived evolutionary tree indicates that the pairs of crs have evolved in concert within each mitochondrial genome. these ... | 2007 | 17000719 |
| generation and distribution of reactive oxygen species in the skin of hairless mice under uva: studies on in vivo chemiluminescent detection and tape stripping methods. | although the formation of reactive oxygen species (ros) in the skin induced by the ultraviolet (uv) light has been shown to lead to many cutaneous disorders, skin cancer and photoageing, the mechanism and distribution of ros generation has not yet been definitively determined. in the present study, we examined the distribution of uva-induced ros in the skin of live hairless mice, using our proposed in vivo imaging chemiluminescent (cl) method to detect ros combined with a cl probe (cypridina hil ... | 2006 | 17002686 |
| luminescent systems in apogonid fishes from the philippines. | luminescence has been discovered in five apogonid fishes from the philippine islands. the luminescent organ systems, which are of two types, are morphologically different from the systems in the japanese cardinal fish, apogon ellioti, and in the apogonid genus siphamia. extracts of the organs all show a luciferin-luciferase type of reaction and cross-react with extracts of apogon ellioti, parapriacanthus ransonneti, and cypridina hilgendorfii. | 1969 | 17834743 |
| imaging of luciferase secretion from transformed chinese hamster ovary cells. | the blue luminescence characteristic of the marine ostracod crustacean vargula hilgendorfii is from a simple, but highly specific, enzyme-substrate reaction. light is emitted by the oxidation of vargula luciferin (substrate) by molecular oxygen, a reaction catalyzed by luciferase. stable transformants of chinese hamster ovary cells carrying the vargula luciferase gene secreted luciferase from discrete sites on the cell surface, and this secretion could be monitored in real time by the biolumines ... | 1992 | 1409669 |
| description of a species of fabaeformiscandona (ostracoda, crustacea) from kushiro marsh, hokkaido, japan, with the nearly complete mitochondrial genomic sequence. | so far, 16 species of non-marine ostracods have been reported from kushiro marsh, kushiro shitsugen national park, eastern hokkaido, japan (hiruta and smith 2001, smith and hiruta 2004). nine of these species are in candonidae, the second-most diverse family of non-marine ostracods. this family contains ca. 550 species, or around 25% of the total number of non-marine ostracod species (martens et al. 2008). | 2015 | 26751633 |