| speciation in the artemia genus: mitochondrial dna analysis of bisexual and parthenogenetic brine shrimps. | from the cloned mitochondrial dnas (mtdnas) isolated from two bisexual species, one mediterranean, artemia salina, and one american, artemia franciscana, and two parthenogenetic (diploid and tetraploid) strains of artemia parthenogenetica collected in spain, physical maps have been constructed and compared. they are extremely different among themselves, much more than the differences between drosophila melanogaster and d. yakuba and in the same range of different mammalian species such as mouse/ ... | 1994 | 8169960 |
| mitochondrial genes collectively suggest the paraphyly of crustacea with respect to insecta. | complete sequences of seven protein coding genes from penaeus notialis mitochondrial dna were compared in base composition and codon usage with homologous genes from artemia franciscana and four insects. the crustacean genes are significantly less a + t-rich than their counterpart in insects and the pattern of codon usage (ratio of g + c-rich versus a + t-rich codon) is less biased. a phylogenetic analysis using amino acid sequences of the seven corresponding polypeptides supports a sister-taxon ... | 1999 | 10368442 |
| satellite dna from the brine shrimp artemia affects the expression of a flanking gene in yeast. | we have previously revealed that in the brine shrimp artemia franciscana an alui dna family of repeats, 113 bp in length, is the major component of the constitutive heterochromatin and that this repetitive dna shows a stable curvature that confers a solenoidal geometry on the double helix in vitro. it was suggested that this particular structure may play a relevant role in determining the condensation of the heterochromatin. in this report we have cloned hexamers of highly-repetitive sequence (a ... | 1997 | 9161405 |
| polymorphism and structure of the gene coding for the alpha 1 subunit of the artemia franciscana na/k-atpase. | genomic clones coding for one of the two identified artemia franciscana na/k-atpase alpha subunits, the alpha 1 subunit, have been isolated. several overlapping clones were obtained, although their restriction maps showed a large heterogeneity. sequencing of their exons showed that they differ in up to 3.46% of their nucleotides in translated regions and 8.18% in untranslated regions. southern blot analysis of dna purified from different lots of a. franciscana cysts and from isolated individuals ... | 1997 | 9020888 |
| partial sequence of the mitochondrial genome of the crustacean daphnia pulex. | a 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean daphnia pulex was sequenced and found to contain the complete genes for eight trnas and five proteins (atp6, atp8, coii, coiii, nd3) and the partial sequence of coi. in combination with data described previously, approximately 50% of the d. pulex mitochondrial genome has been sequenced. the gene order in this half of the genome is identical to that of drosophila yakuba which differs from that of the other completely se ... | 1997 | 9000380 |
| accumulation of trimethoprim, sulfamethoxazole, and n-acetylsulfamethoxazole in fish and shrimp fed medicated artemia franciscana. | in a previous paper (h.j. nelis, p. léger, p. sorgeloos, and a. p. de leenheer, antimicrob. agents chemother. 35:2486-2489, 1991) it was reported that two selected antibacterial agents, i.e., trimethoprim and sulfamethoxazole, can be efficiently bioencapsulated in nauplii of the brine shrimp artemia franciscana for administration to fish. this follow-up study showed that larvae of the sea bass and the turbot as well as postlarvae of the white shrimp accumulate the therapeutic agents in high quan ... | 1996 | 8807056 |
| effect of advanced oxidation processes on the toxicity of municipal landfill leachates. | the aim of the present study was to assess the effect of advanced oxidation processes (aops) (oxidation ozone and peroxide/ozone) on the toxicity of leachates from municipal landfill for warsaw, poland, using a battery of tests. aops used to pre-treat leachates were carried out in laboratory conditions after their coagulation with the use of fecl3. the effects of the pre-treatment of leachates using the method of coagulation with fecl3 depended on the concentration of organic compounds and with ... | 2004 | 15077983 |
| the sequence, organization, and evolution of the locusta migratoria mitochondrial genome. | the sequencing of the cloned locusta migratoria mitochondrial genome has been completed. the sequence is 15,722 bp in length and contains 75.3% a+t, the lowest value in any of the five insect mitochondrial sequences so far determined. the protein coding genes have a similar a+t content (74.1%) but are distinguished by a high cytosine content at the third codon position. the gene content and organization are the same as in drosophila yakuba except for a rearrangement of the two trna genes trnalys ... | 1995 | 8587138 |
| regulatory features of protein synthesis in isolated mitochondria from artemia embryos. | optimal conditions were developed for measuring rates of protein synthesis in isolated mitochondria from encysted embryos of artemia franciscana to 1) identify the required chemical constituents, 2) assess the influence of extramitochondrial ph on protein synthesis, and 3) investigate potential mechanisms coordinating nuclear and mitochondrial gene expression. isolation procedures resulted in intact, highly coupled mitochondria [respiratory control ratio = 6.48 +/- 0.43 (se), n = 21]. requiremen ... | 1993 | 8285263 |
| structure of artemia franciscana sarco/endoplasmic reticulum ca-atpase gene. | genomic clones coding for the artemia franciscana sarco(endo)plasmic reticulum ca-atpase have been isolated. the restriction map of the overlapping clones covers a region of 65 kilobases of dna. nucleotide sequence of mrna coding regions shows that the gene is divided into 18 exons separated by 17 introns. compared with the structure of the rabbit sarco(endo)plasmic reticulum ca-atpase 1 gene, 12 of the introns are in the same position, 8 introns present in the rabbit gene are absent from a. fra ... | 1994 | 8175720 |
| using bioassays for testing seawater quality in greece. | the objective of this work was the assessment of seawater quality in thermaikos gulf, pagassitikos gulf and skiathos island in northern aegean sea by the use of bioassays. two bioassays using marine organisms as indicators of seawater quality were applied in this study; the invertebrate artemia franciscana and the marine bioluminescent bacterium vibrio fischeri. bioassays are required for the integrated evaluation of water pollution, as physical and chemical tests alone are not sufficient enough ... | 2003 | 12680581 |
| the toxicity of cationic surfactants in four bioassays. | the purpose of this study was to investigate the toxicity of 15 quaternary ammonium compounds (qacs) in a battery of four bioassays comprising the bacterium vibrio fischeri, two ciliated protozoa spirostomum ambiguum and tetrahymena thermophia, and the anostracean crustacean artemia franciscana. the compounds were prepared by professor pernak's group at poznań university of technology (poland). the toxicity of the test compounds was very high, with ec(lc)(50) values varying from 0.11 to 70 micro ... | 2003 | 12547639 |
| complete mitochondrial dna sequence of a tadpole shrimp (triops cancriformis) and analysis of museum samples. | the complete mitochondrial dna (mtnda) of the tadpole shrimp triops cancriformis was sequenced. the sequence consisted of 15,101 bp with an a+t content of 69%. its gene arrangement was identical with those sequences of the water flea (daphnia pulex) and giant tiger prawn (penaeus monodon), whereas it differed from that of the brine shrimp (artemia franciscana) in the arrangement of its genes for trnas. phylogenetic analysis revealed t. cancriformis to be more closely related to the water flea th ... | 2002 | 12481263 |
| effects of bacteria on artemia franciscana cultured in different gnotobiotic environments. | the use of probiotics is receiving considerable attention as an alternative approach to control microbiota in aquaculture farms, especially in hatching facilities. however, application with consistent results is hampered by insufficient information on their modes of action. to investigate whether dead bacteria (allowing investigation of their nutritional effect) or live bacteria (allowing evaluation of their probiotic effect) have any beneficial effect towards artemia franciscana and, subsequent ... | 2005 | 16085818 |
| [allozymatic variation in crustacea artemia franciscana (anostraca: artemiidae) of freat salt lake in different experimental conditions]. | starch gel electrophoresis was used to analyze the allelic variability of four polymorphic loci (lap-2, lap-3, pgm and gpi) from a single population of artemia franciscana (kellogg, 1906) from the great salt lake (utah, usa), cultured under eight different experimental conditions. the organisms were cultured to the adult stage under a 2 x 2 x 2 experimental design (22 and 30 degrees c; 30 and 60 ppt salinity; and dunaliella sp. and spirulina sp. as food). there were significant differences in al ... | 2002 | 12298270 |
| exiguobacterium mexicanum sp. nov. and exiguobacterium artemiae sp. nov., isolated from the brine shrimp artemia franciscana. | two gram-positive strains isolated from cysts of the brine shrimp artemia franciscana were subjected to a polyphasic taxonomic analysis. based on 16s rrna gene sequence comparison and composition of isoprenoid quinones, peptidoglycan and fatty acids, these organisms are members of the genus exiguobacterium. both strains showed 95.9% 16s rrna gene sequence similarity to one another. the 16s rrna gene sequences of strain 8n(t) and 9an(t) were 97.5% and 98.9% similar to those of exiguobacterium aur ... | 2006 | 16564954 |
| isolation of prorocentrum lima (syn. exuviaella lima) and diarrhetic shellfish poisoning (dsp) risk assessment in the gulf of california, mexico. | a benthic toxic dinoflagellate identified as prorocentrum lima (syn. exuviaella lima), and designated as strain prl-1, was isolated from the coast of el pardito (coyote) island in baja california sur, mexico, after a fisherman poisoning incident involving consumption of liver from lutjanus colorado, and mycteroperca prionura fish. purification and culturing was done in es-si medium, under 12:12 light/dark cycle (4 x 20 w cool-white fluorescent lamps), at 22 degrees c and constant stirring during ... | 2002 | 12165314 |
| quorum sensing-disrupting brominated furanones protect the gnotobiotic brine shrimp artemia franciscana from pathogenic vibrio harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates. | autoinducer 2 (ai-2) quorum sensing was shown before to regulate the virulence of vibrio harveyi towards the brine shrimp artemia franciscana. in this study, several different pathogenic v. harveyi, vibrio campbellii, and vibrio parahaemolyticus isolates were shown to produce ai-2. furthermore, disruption of ai-2 quorum sensing by a natural and a synthetic brominated furanone protected gnotobiotic artemia from the pathogenic isolates in in vivo challenge tests. | 2006 | 16957276 |
| transactivation domains are not functionally conserved between vertebrate and invertebrate serum response factors. | the transcription factor serum response factor (srf) regulates expression of growth factor-dependent genes and muscle-specific genes in vertebrates. homologous factors regulate differentiation of some ectodermic tissues in invertebrates. to explore the molecular basis of these different physiological functions, the functionality of human, drosophila melanogaster and artemia franciscana srfs in mammalian cells has been compared in this article. d. melanogaster and, to a lesser extend, a. francisc ... | 2002 | 12153563 |
| functional analysis of a small heat shock/alpha-crystallin protein from artemia franciscana. oligomerization and thermotolerance. | oviparously developing embryos of the brine shrimp, artemia franciscana, synthesize abundant quantities of a small heat shock/alpha-crystallin protein, termed p26. wild-type p26 functions as a molecular chaperone in vitro and is thought to help encysted artemia embryos survive severe physiological stress encountered during diapause and anoxia. full-length and truncated p26 cdna derivatives were generated by pcr amplification of p26-3-6-3, then cloned in either pet21(+) or prsetc and expressed in ... | 2002 | 11846795 |
| salt toxicosis in ruddy ducks that winter on an agricultural evaporation basin in california. | agricultural evaporation basins are used as a means to dispose of highly saline underground-tile-drainage water in the san joaquin valley (california, usa). the hypersaline water conditions encourage high aquatic invertebrate production, primarily brine shrimp (artemia franciscana), which attract birds to these sites. cool winter temperatures (< 4 c) and hypersaline water conditions (> 70,000 mumhos/cm) resulted in feather salt encrustation and salt toxicosis in ruddy ducks (oxyura jamaicensis). ... | 2002 | 11838203 |
| development of new heparin-like compounds and other antithrombotic drugs and their interaction with vascular endothelial cells. | the anticlotting and antithrombotic activities of heparin, heparan sulfate, low molecular weight heparins, heparin and heparin-like compounds from various sources used in clinical practice or under development are briefly reviewed. heparin isolated from shrimp mimics the pharmacological activities of low molecular weight heparins. a heparan sulfate from artemia franciscana and a dermatan sulfate from tuna fish show a potent heparin cofactor ii activity. a heparan sulfate derived from bovine panc ... | 2001 | 11378657 |
| identification of the s6 kinase activity stimulated in quiescent brine shrimp embryos upon entry to preemergence development as p70 ribosomal protein s6 kinase: isolation of artemia franciscana p70s6k cdna. | we previously demonstrated that a protein kinase responsible for phosphorylating 40s ribosomal subunits is activated in quiescent artemia franciscana embryos within 15 min of restoration of normal tonicity and incubation at 30 degrees c. here, we identify the activated s6 kinase as a. franciscana p70 ribosomal s6 kinase (p70s6k) subsequent to the isolation of an artemia p70s6k cdna. the protein conceptually translated from cdna has 70% similarity and 64% identity to both drosophila melanogaster ... | 2001 | 11310561 |
| uptake and processing of a vibrio anguillarum bacterin in artemia franciscana measured by elisa and immunohistochemistry. | nauplii of artemia franciscana were incubated in two different concentrations (undiluted and 1:9 in autoclaved sea water) of a divalent bacterin composed of two different serovars of vibrio anguillarum. in order to investigate uptake and further processing of a bacterin in the live feed organism a. franciscana, immunohistochemistry was applied, visualising the presence of whole bacterial cells and antigens from the bacterin in individual nauplii. by using elisa, it was shown that approximately 1 ... | 2001 | 11271599 |
| characterization of a novel heterodimeric cathepsin l-like protease and cdna encoding the catalytic subunit of the protease in embryos of artemia franciscana. | embryos and larvae of the brine shrimp, artemia franciscana, contain a novel cathepsin l-like cysteine protease (acp) composed of 28.5- and 31.5-kda subunits. both subunits of the acp are glycosylated, and seven isoforms of the protease were identified by isoelectric focusing with pi values ranging from 4.6 to 6.2. several clones containing sequences coding for the 28.5-kda subunit of the acp were isolated from an artemia embryo cdna library in lambda zap ii. one clone of 1229 bp, with an open r ... | 2001 | 11235917 |
| cloning and characterization of sarco/endoplasmic reticulum ca(2+)-atpase (serca) from crayfish axial muscle. sarco/endoplasmic reticulum ca(2+)-atpase. | the discontinuous pattern of muscle growth during the moulting cycle of a freshwater crustacean (the crayfish procambarus clarkii) was used as a model system to examine the regulation of the expression of sarco/endoplasmic reticulum ca(2+)-atpase (serca). we describe the cloning, sequencing and characterization of a novel serca cdna (3856 bp) obtained from crayfish axial abdominal muscle by reverse transcription/polymerase chain reaction (rt-pcr) followed by rapid amplification of cdna ends (rac ... | 2000 | 11044380 |
| a serum response factor homologue is expressed in ectodermal tissues during development of the crustacean artemia franciscana. | complementary dna clones have been isolated from the crustacean artemia franciscana coding for a serum response factor (srf)-homologue that is more than 96% identical to human and drosophila melanogaster srfs in their mads boxes. the srf homologue is expressed in ectodermal tissues, as determined by in situ hybridization experiments. a srf-binding site has been identified in the promoter region of the actin403 gene that is also expressed in ectodermal tissues, in accordance with its transcriptio ... | 2000 | 10960789 |
| a novel heparan sulphate with high degree of n-sulphation and high heparin cofactor-ii activity from the brine shrimp artemia franciscana. | with the aid of heparinase and heparitinases from flavobacterium heparinum and 13c and ih nmr spectroscopy it was shown that the heparan sulphate isolated from the brine shrimp artemia franciscana exhibits structural features intermediate between those of mammalian heparins and heparan sulphates. these include an unusually high degree of n-sulphation (with corresponding very low degree of n-acetylation), a relatively high content of iduronic acid residues (both unsulphated and 2-o-sulphated) and ... | 2000 | 10704986 |
| interactions of the ccaat-binding trimer nf-y with nucleosomes. | nf-y is a sequence-specific evolutionary conserved activator binding to ccaat boxes with high affinity and specificity. it is a trimer formed by nf-ya and two putative histone-like subunits, nf-yb and nf-yc, showing similarity to histones h2b and h2a, respectively. we investigated the relationships between nf-y and chromatin using an artemia franciscana chromatin assembly system with plasmids containing the major histocompatibility complex class ii ea promoter. the nf-y trimer, but not single su ... | 1999 | 9880503 |
| characterization of gamma-tubulin in artemia: isoform composition and spatial distribution in polarized cells of the larval epidermis. | microtubule arrangement is influenced by gamma-tubulin, a soluble protein of the eukaryotic cell cytosol and a component of microtubule-organizing centers. in this study, affinity purified antibodies to gamma-tubulin were prepared and their specificity demonstrated by immunostaining of western blots and in competitive elisas. when employed to label mouse fibroblasts, one or two brightly stained dots appeared in each cell, a pattern characteristic of centrosomes. antibody 9, raised to a conserved ... | 1998 | 9712263 |
| bioencapsulation of two different vibrio species in nauplii of the brine shrimp (artemia franciscana) | two groups of nauplii from the brine shrimp (artemia franciscana) were enriched with different bacteria, and the dynamics of bacterial uptake by the nauplii were observed. this study showed that the efficiency of artemia nauplii in bioencapsulating bacteria strongly depends on the type of bacteria used, time of exposure, and status (live or dead) of the bacteria. | 1998 | 9603861 |
| virulence of luminous vibrios to artemia franciscana nauplii. | from healthy and diseased penaeid shrimp from asia and the americas, 25 luminous and 2 non-luminous bacterial strains were isolated, and 14 were phenotypically identified as vibrio harveyi; 9 isolates produced significant mortalities (45 to 80%) in artemia franciscana nauplii at inoculation densities of 10(5) to 10(6) cfu ml(-1) compared to the controls (unchallenged nauplii). the maximum number of bacteria ingested (bioencapsulated) by the artemia nauplii varied from less than 10 to 10(3) cfu n ... | 2003 | 12691194 |
| a 49 kda microtubule cross-linking protein from artemia franciscana is a coenzyme a-transferase. | embryos and larvae of the brine shrimp, artemia franciscana, were shown previously to possess a protein, now termed p49, which cross-links microtubules in vitro. molecular characteristics of p49 were described, but the protein's identity and its role in the cell were not determined. degenerate oligonucleotide primers designed on the basis of peptide sequence obtained by edman degradation during this study were used to generate p49 cdnas by rt-pcr and these were cloned and sequenced. comparison w ... | 2003 | 14653822 |
| white spot syndrome virus (wssv) infectivity for artemia at different developmental stages. | white spot syndrome virus (wssv) is a major pathogen of cultivated shrimp, but its host range includes a large number of crustaceans. in this investigation, artemia franciscana was tested for susceptibility to wssv by the oral route. both instars and adults were challenged, and the presence of wssv was followed through to reproductive cysts and offspring using pcr. wssv caused a much lower cumulative mortality in artemia than in cultivated shrimp by 10 d post-challenge. instars, adults and repro ... | 2003 | 14960040 |
| phylogeography and local endemism of the native mediterranean brine shrimp artemia salina (branchiopoda: anostraca). | there has been a recent appreciation of the ecological impacts of zooplanktonic species invasions. the north american brine shrimp artemia franciscana is one such alien invader in hyper-saline water ecosystems at a global scale. it has been shown to outcompete native artemia species, leading to their local extinction. we used partial sequences of the mitochondrial cytochrome c oxidase subunit 1 (coi or cox1) gene to investigate the genetic diversity and phylogeography of a. salina, an extreme ha ... | 2008 | 18510585 |
| a small heat-shock protein, p26, from the crustacean artemia protects mammalian cells (cos-1) against oxidative damage. | a small heat-shock protein (p26) purified from stress-resistant embryos of the crustacean, artemia franciscana, was introduced into cultured cells of green monkey kidney (cos-1) using the bioporter delivery system. cells containing p26 exhibited impressive resistance to hydrogen peroxide compared to controls. introduction of the disaccharide trehalose did not provide protection against oxidative damage, but enhanced substantially the protective performance of p26 when both were present. these st ... | 2004 | 15223021 |
| a short-term sublethal in situ sediment assay with chironomus riparius based on postexposure feeding. | a short-term, sublethal, and cost-effective in situ sediment toxicity assay for routine assessments with the midge chironomus riparius meigen, based on postexposure feeding, was developed and evaluated. an inexpensive and easy-to-use assay chamber was designed. a sediment toxicity assay was successfully performed at a lentic system impacted by acid mine drainage, at sites with different types of sediment. it consisted of a 48-h exposure period followed by a 1-h postexposure feeding during which ... | 2005 | 16001149 |
| the impact of mutations in the quorum sensing systems of aeromonas hydrophila, vibrio anguillarum and vibrio harveyi on their virulence towards gnotobiotically cultured artemia franciscana. | disruption of quorum sensing, bacterial cell-to-cell communication by means of small signal molecules, has been suggested as a new anti-infective strategy for aquaculture. however, data about the impact of quorum sensing on the virulence of aquatic pathogens are scarce. in this study, a model system using gnotobiotically cultured artemia franciscana was developed in order to determine the impact of mutations in the quorum sensing systems of aeromonas hydrophila, vibrio anguillarum and v. harveyi ... | 2005 | 16011761 |
| biological activity of neosergeolide and isobrucein b (and two semi-synthetic derivatives) isolated from the amazonian medicinal plant picrolemma sprucei (simaroubaceae). | in the present study, in vitro techniques were used to investigate a range of biological activities of known natural quassinoids isobrucein b (1) and neosergeolide (2), known semi-synthetic derivative 1,12-diacetylisobrucein b (3), and a new semi-synthetic derivative, 12-acetylneosergeolide (4). these compounds were evaluated for general toxicity toward the brine shrimp species artemia franciscana, cytotoxicity toward human tumour cells, larvicidal activity toward the dengue fever mosquito vecto ... | 2009 | 19274376 |
| characterization of novel sequence motifs within n- and c-terminal extensions of p26, a small heat shock protein from artemia franciscana. | the small heat shock proteins function as molecular chaperones, an activity often requiring reversible oligomerization and which protects against irreversible protein denaturation. an abundantly produced small heat shock protein termed p26 is thought to contribute to the remarkable stress resistance exhibited by encysted embryos of the crustacean, artemia franciscana. three novel sequence motifs termed g, r and ts were individually deleted from p26 by site-directed mutagenesis. g encompasses res ... | 2005 | 16218954 |
| conservation of arthropod midline netrin accumulation revealed with a cross-reactive antibody provides evidence for midline cell homology. | although many similarities in arthropod cns development exist, differences in axonogenesis and the formation of midline cells, which regulate axon growth, have been observed. for example, axon growth patterns in the ventral nerve cord of artemia franciscana differ from that of drosophila melanogaster. despite such differences, conserved molecular marker expression at the midline of several arthropod species indicates that midline cells may be homologous in distantly related arthropods. however, ... | 2009 | 19469853 |
| inhibition of apoptosis by p26: implications for small heat shock protein function during artemia development. | p26, an abundantly expressed small heat shock protein, is thought to establish stress resistance in oviparously developing embryos of the crustacean artemia franciscana by preventing irreversible protein denaturation, but it might also promote survival by inhibiting apoptosis. to test this possibility, stably transfected mammalian cells producing p26 were generated and their ability to resist apoptosis induction determined. examination of immunofluorescently stained transfected 293h cells by con ... | 2006 | 16572731 |
| non-lethal heat shock protects gnotobiotic artemia franciscana larvae against virulent vibrios. | brine shrimp artemia were exposed under gnotobiotic conditions to a non-lethal heat shock (nlhs) from 28 to 32, 37 and 40 degrees c. different recovery periods (2, 6, 12 and 24h) and different heat-exposure times (15, 30, 45 and 60 min) were tested. after these nlhs, artemia was subsequently challenged with vibrio. challenge tests were performed in stressed and unstressed nauplii at concentrations of 10(7) cells ml(-1) of pathogenic bacteria, vibrio campbellii and vibrio proteolyticus. a nlhs wi ... | 2007 | 17123831 |
| a biometric and ecologic comparison between artemia from mexico and chile. | a preliminary biometric and ecologic database for the brine shrimp artemia from mexico and chile is presented. the area abounds in small and seasonal ponds and large inland lakes, the latter mainly located in mexico, although relatively large and isolated lakes are found in complex hydrological settings in pre-high plateau areas of chile. this paper summarizes research efforts aimed at the localization, characterization, and evaluation of the aquaculture potential of artemia populations in mexic ... | 2006 | 17125517 |
| quantitative oral dosing of water soluble and lipophilic contaminants in the japanese medaka (oryzias latipes). | quantitative oral dosing in fish can be challenging, particularly with water soluble contaminants, which can leach into the aquarium water prior to ingestion. we applied a method of bioencapsulation using newly hatched brine shrimp (artemia franciscana) nauplii to study the toxicokinetics of five chlorinated and brominated halogenated acetic acids (haas), which are drinking water disinfection by-products. these results are compared to those obtained in a previous study using a polybrominated dip ... | 2007 | 17188578 |
| the bacterial storage compound poly-beta-hydroxybutyrate protects artemia franciscana from pathogenic vibrio campbellii. | infections caused by antibiotic-resistant luminescent vibrios can cause dramatic losses in aquaculture. in this study, the short-chain fatty acid beta-hydroxybutyrate and its polymer poly-beta-hydroxybutyrate were investigated as possible new biocontrol agents. beta-hydroxybutyrate was shown to completely inhibit the growth of pathogenic vibrio campbelli at 100 mm. moreover, the addition of 100 mm of this fatty acid to the culture water of artemia nauplii infected with the v. campbelli strain si ... | 2007 | 17222142 |
| caspase activity during cell stasis: avoidance of apoptosis in an invertebrate extremophile, artemia franciscana. | evaluation of apoptotic processes downstream of the mitochondrion reveals caspase-9- and low levels of caspase-3-like activities in partly purified extracts of artemia franciscana embryos. however, in contrast to experiments with extracts of human hepatoma cells, cytochrome c fails to activate caspase-3 or -9 in extracts from a. franciscana. furthermore, caspase-9 activity is sensitive to exogenous calcium. the addition of 5 mm calcium leads to a 4.86 +/- 0.19 fold (sd) (n = 3) increase in activ ... | 2007 | 17255212 |
| functional characterization of artemin, a ferritin homolog synthesized in artemia embryos during encystment and diapause. | oviparously developing embryos of the crustacean artemia franciscana encyst and enter diapause, exhibiting a level of stress tolerance seldom seen in metazoans. the extraordinary stress resistance of encysted artemia embryos is thought to depend in part on the regulated synthesis of artemin, a ferritin superfamily member. the objective of this study was to better understand artemin function, and to this end the protein was synthesized in escherichia coli and purified to apparent homogeneity. pur ... | 2007 | 17257268 |
| [composition of fat acids in three mexican populations of artemia franciscana from epicontinental waters]. | in this paper is presented the percentage of fatty acids composition of three artemia franciscana mexican populations of epicontinentals waters; two are from natural environments (coahuila and san luis potosf) and one (texcoco) is a culture fed with spirulina. determination of fatty acids composition in each population, was performed by extraction of total lipid by the soxhlet method and the fatty acids methyl esters were determined by gas chromatography. the results show that artemia of texcoco ... | 2004 | 17357427 |
| formation of boldenone and boldenone-analogues by maggots of lucilia sericata. | current evidence suggests that neo formation of the anabolic steroid boldenone (androsta-1,4-diene-17-ol-3-one) occurs in calves' faecal material, making it difficult to distinguish between illegally administered boldenone and its potential endogenous presence. this strengthens the urgent need to elucidate the pathway leading to boldenone formation. in our laboratory, the invertebrate neomysis integer (crustacea, mysidacea) was used since 2004 as an alternative model for the partial replacement ... | 2007 | 17386708 |
| poly-beta-hydroxybutyrate-accumulating bacteria protect gnotobiotic artemia franciscana from pathogenic vibrio campbellii. | a poly-beta-hydroxybutyrate (phb)-accumulating enrichment culture was obtained using activated sludge from a polyphosphate-accumulating reactor as inoculum. phb accumulated by the enrichment culture significantly enhanced the survival of artemia nauplii, infected with the virulent pathogen vibrio campbellii lmg 21363. a strain was isolated from the enrichment culture, based on its ability to accumulate phb, and 16s rrna gene sequencing of the isolate revealed 99% sequence similarity to brachymon ... | 2007 | 17391334 |
| predicted sub-populations in a marine shrimp proteome as revealed by combined est and cdna data from multiple penaeus species. | abstract: | 2010 | 21067619 |
| possible involvement of artemia as live diet in the transmission of cryptosporidiosis in cultured fish. | the viability of cryptosporidium parvum oocysts ingested by artemia franciscana metanauplii was evaluated using two fluorogenic vital dyes. there was no significant difference (p = 0.09) between the viability of oocysts maintained in saline (control) and those recovered from the digestive tract of the microcrustacean 24 h after ingestion (95 vs 90% viable oocysts). the results suggest that artemia, used as a life food in fish larviculture, may act as a vehicle for transmission of piscine cryptos ... | 2007 | 17468970 |
| expression of virulence genes in luminescent and nonluminescent isogenic vibrios and virulence towards gnotobiotic brine shrimp (artemia franciscana). | this study aimed to evaluate the expression levels of virulence gene regulators (luxr and toxr) and virulence factors (serine protease, metalloprotease and haemolysin) in luminescent and nonluminescent isogenic vibrio harveyi and vibrio campbellii. | 2010 | 21091862 |
| a distinct sequence in the adenine nucleotide translocase from artemia franciscana embryos is associated with insensitivity to bongkrekate and atypical effects of adenine nucleotides on ca2+ uptake and sequestration. | mitochondria isolated from embryos of the crustacean artemia franciscana lack the ca(2+)-induced permeability transition pore. although the composition of the pore described in mammalian mitochondria is unknown, the impacts of several effectors of the adenine nucleotide translocase (ant) on pore opening are firmly established. notably, adp, atp and bongkrekate delay, whereas carboxyatractyloside hastens, ca(2+)-induced pore opening. here, we report that adenine nucleotides decreased, whereas car ... | 2011 | 21205213 |
| cestode parasitism in invasive and native brine shrimps (artemia spp.) as a possible factor promoting the rapid invasion of a. franciscana in the mediterranean region. | artemia franciscana is an invasive crustacean expanding its range in hypersaline wetlands in the mediterranean region and replacing native artemia parthenogenetica and artemia salina. native brine shrimps are known as intermediate hosts of cestodes; infected individuals exhibit changes in their behaviour and appearance, thus facilitating the parasite transmission to the avian hosts by predation. to assess whether invasive brine shrimps participate in the cestode life cycles to the same extent as ... | 2007 | 17712569 |
| developmentally regulated synthesis of p8, a stress-associated transcription cofactor, in diapause-destined embryos of artemia franciscana. | diapause-destined embryos of the crustacean artemia franciscana arrest as gastrulae, acquire extreme stress tolerance, and enter profound metabolic dormancy. among genes upregulated at 2 days postfertilization in these embryos is a homologue of p8, a stress-inducible transcription cofactor. artemia p8 is smaller than vertebrate homologues but shares a basic helix-loop-helix domain and a bipartite nuclear localization signal. probing of restriction digested dna on southern blots indicated a singl ... | 2007 | 17915558 |
| gene expression in diapause-destined embryos of the crustacean, artemia franciscana. | diapause-destined embryos of the crustacean artemia franciscana cease development as gastrulae, encyst, and enter a resting stage characterized by greatly reduced metabolic activity and extreme stress resistance. to better understand diapause induction and maintenance in artemia embryos gene expression was analyzed by subtractive hybridization at two days post-fertilization, a time early in this developmental process. eighty-five of 264 cdna clones sequenced matched genbank entries and they fell ... | 2007 | 17950581 |
| regulation and function of lysine-substituted na,k pumps in salt adaptation of artemia franciscana. | the brine shrimp artemia thrives at extreme conditions of up to 300 g/l salt in hypersaline lakes, but the molecular aspects of this salt adaptation are not clarified. to examine the influence of salt on the expression of two isoforms of na,k-atpase, adult artemia franciscana were cultured for 39 days with the microalga dunaliella salina as fodder at increasing salt from 30 to 280 g/l. quantitative reverse-transcriptase polymerase chain reaction showed that the abundance of mrna of the lysine-su ... | 2008 | 18074167 |
| exposure of gnotobiotic artemia franciscana larvae to abiotic stress promotes heat shock protein 70 synthesis and enhances resistance to pathogenic vibrio campbellii. | larvae of the brine shrimp artemia franciscana serve as important feed in fish and shellfish larviculture; however, they are subject to bacterial diseases that devastate entire populations and consequently hinder their use in aquaculture. exposure to abiotic stress was shown previously to shield artemia larvae against infection by pathogenic vibrio, with the results suggesting a mechanistic role for heat shock protein 70. in the current report, combined hypothermic/hyperthermic shock followed by ... | 2008 | 18347942 |
| [biological and biochemical quality of the artemia (anostraca: artemiidae) population from real de salinas saltworks, calkiní, campeche, mexico]. | cysts of artemia spp. collected from february 1997 to february 2000 in the real de salinas solar saltworks, campeche, mexico, were compared with artemia franciscana (batch number 8,131 microfeast artemia cysts, texas, usa). the variables determined in these two populations were: number of cysts per gram, hatching percentage, hatching efficiency, hatching rate, hatching synchrony and hatching biomass, as well as diameter of the cysts and length of the nauplii (instar i). for salinas, the average ... | 2006 | 18457164 |
| oligomerization, chaperone activity, and nuclear localization of p26, a small heat shock protein from artemia franciscana. | artemia franciscana embryos undergo encystment, developmental arrest and diapause, the last characterized by profound metabolic dormancy and extreme stress resistance. encysted embryos contain an abundant small heat shock protein termed p26, a molecular chaperone that undoubtedly has an important role in development. to understand better the role of p26 in artemia embryos, the structural and functional characteristics of full-length and truncated p26 expressed in escherichia coli and cos-1 cells ... | 2004 | 15258152 |
| mitochondrial permeability transition in the crustacean artemia franciscana: absence of a calcium-regulated pore in the face of profound calcium storage. | when mammalian mitochondria are exposed to high calcium and phosphate, a massive swelling, uncoupling of respiration, and release of cytochrome c occur. these changes are mediated by opening of the mitochondrial permeability transition pore (mptp). activation of the mptp in vivo in response to hypoxic and oxidative stress leads to necrotic and apoptotic cell death. considering that embryos of the brine shrimp artemia franciscana tolerate anoxia for years, we investigated the mptp in this crustac ... | 2005 | 15718386 |
| comparison of several toxicity tests applied to complex wastewaters and mussel biomarkers in receiving waters. | in this study, the complex wastewaters/effluents discharged to coastal regions of turkey and greece were sampled, and various toxicity tests were applied. the bioassays used included the assessment of the luminescence inhibition of the bacterium vibrio fisheri, the photosynthesis inhibition (14c uptake rate) and growth inhibition of the algal species phaeodactylum tricornutum, and the mortality of the crustacean artemia franciscana and rotifer brachionus plicatilis. additionally, "neutral red re ... | 2005 | 15991721 |
| occurrence of mitochondria-targeted late embryogenesis abundant (lea) gene in animals increases organelle resistance to water stress. | anhydrobiotic animals survive virtually complete loss of cellular water. the mechanisms that explain this phenomenon are not fully understood but often include the accumulation of low molecular weight solutes such as trehalose and macromolecules like late embryogenesis abundant (lea) proteins. here we report for the first time the occurrence of a mitochondria-targeted lea gene (afrlea3m) product in an animal species. the deduced molecular mass of the 307-amino acid polypeptide from the brine shr ... | 2009 | 19228698 |
| a comparison of the antimicrobial activity and toxicity of six combretum and two terminalia species from southern africa. | plants of the family combretaceae are amongst the most widely used plants for traditional medicinal purposes in southern africa. in particular, many species of combretum and terminalia are used for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities, yet their antimicrobial potential has not been rigorously studied and compared. | 2015 | 25709234 |
| ingestion of bacteria overproducing dnak attenuates vibrio infection of artemia franciscana larvae. | feeding of bacterially encapsulated heat shock proteins (hsps) to invertebrates is a novel way to limit vibrio infection. as an example, ingestion of escherichia coli overproducing prokaryotic hsps significantly improves survival of gnotobiotically cultured artemia larvae upon challenge with pathogenic vibrio campbellii. the relationship between hsp accumulation and enhanced resistance to infection may involve dnak, the prokaryotic equivalent to hsp70, a major molecular chaperone in eukaryotic c ... | 2009 | 19373565 |
| feeding artemia franciscana (kellogg) larvae with bacterial heat shock protein, protects from vibrio campbellii infection. | among their numerous physiological effects, heat shock proteins (hsps) are potent immunomodulators, a characteristic reflecting their potential as therapeutic agents and which led to their application in combating infection. as an example, the up-regulation of endogenous hsp70 in the branchiopod crustacean artemia franciscana (kellogg) is concurrent with shielding against bacterial infection. to better understand this protective mechanism, gnotobiotic artemia were fed with escherichia coli treat ... | 2009 | 19515074 |
| experimental exposure of artemia to hepatopancreatic parvo-like virus and subsequent transmission to post-larvae of penaeus monodon. | the different life stages of artemia franciscana were experimentally exposed to hepatopancreatic parvo-like virus (hpv), in order to evaluate the possibility of artemia acting as reservoir or carrier for hpv. all the five developmental stages of artemia were challenged with hpv both by immersion and oral infection routes. the viral infectivity to artemia was studied by pcr but not much difference in mortality between control and challenge groups were observed. to confirm the vector status of art ... | 2009 | 19666028 |
| development of paratransgenic artemia as a platform for control of infectious diseases in shrimp mariculture. | to study the accumulation and retention of recombinant proteins in artemia gut for optimizing paratransgenic disease control in shrimp aquaculture. | 2010 | 19702854 |
| protective efficacy of the antioxidants vitamin e and trolox against microcystis aeruginosa and microcystin-lr in artemia franciscana nauplii. | this study was undertaken to evaluate the protective efficacy of the antioxidants vitamin e and trolox (a water-soluble vitamin e derivative) against the toxicity of microcystin-lr (mc-lr), microcystis aeruginosa aqueous extract (ce), and a reference toxin, menadione sodium bisulfite (msb), in artemia franciscana nauplii. this was achieved by using the well-established brine shrimp bioassay. the experiment was conducted in 2 stages, with (1) 12-h mortality time course and (2) lc50 determination ... | 2009 | 20077231 |
| evaluation of toxicity of polluted marine sediments from bahia salina cruz, mexico. | bahia salina cruz, oaxaca, mexico is a major center of oil and refined product distribution on the mexican pacific coast. from the start of oil industry operations in 1979, negative effects from discharges of treated effluents in the bay have been a constant concern for local communities. we analyzed 28 surface sediment samples obtained in june, 2002 to evaluate the level of toxicity in the littoral zone, port-harbor, and la ventosa estuary in bahia salina cruz. the extractable organic matter co ... | 2010 | 20390851 |
| the application of bioflocs technology to protect brine shrimp (artemia franciscana) from pathogenic vibrio harveyi. | to study the potential biocontrol activity of bioflocs technology. | 2010 | 20629797 |
| efficacy of heterologous and homologous heat shock protein 70s as protective agents to artemia franciscana challenged with vibrio campbellii. | the hsp70 class of heat shock proteins (hsps) has been implicated at multiple points in the immune response of both vertebrates and invertebrates. this class of chaperones is highly conserved in both sequence and structure, from prokaryotes to higher eukaryotes. in view of their high degree of homology, it was assumed that these hsp70 proteins derived either from the prokaryotes or eukaryotes would have similar functions, especially in relation to their protective ability in a challenge assay. t ... | 2010 | 20643210 |
| resorcinol degradation by a penicillium chrysogenum strain under osmotic stress: mono and binary substrate matrices with phenol. | a phenol-degrading penicillium chrysogenum strain previously isolated from a salt mine was able to grow at 1,000 mg l(-1) of resorcinol on solid medium. the aerobic degradation of resorcinol by p. chrysogenum clona2 was studied in batch cultures in minimal mineral medium with 58.5 g l(-1) of sodium chloride using resorcinol as the sole carbon source. the fungal strain showed the ability to degrade up to 250 mg l(-1) of resorcinol. resorcinol and phenol efficiency degradation by p. chrysogenum cl ... | 2011 | 20859653 |
| in vitro and in vivo expression of virulence genes in vibrio isolates belonging to the harveyi clade in relation to their virulence towards gnotobiotic brine shrimp (artemia franciscana). | vibrios belonging to the harveyi clade are pathogenic marine bacteria affecting both vertebrates and invertebrates, thereby causing a severe threat to the aquaculture industry. in this study, the expression of haemolysin, metalloprotease, serine protease, the quorum sensing master regulator luxr and the virulence regulator toxr in different harveyi clade isolates was measured with reverse transcriptase real-time pcr with specific primers. there was relatively low variation in the in vitro expres ... | 2010 | 20946530 |
| stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of aeromonas hydrophila on the digestive tract of germ-free artemia franciscana nauplii. | validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of aeromonas hydrophila on the gut development of germ-free artemia franciscana nauplii. | 2010 | 21040270 |
| trophic transfer of lead through a model marine four-level food chain: tetraselmis suecica, artemia franciscana, litopenaeus vannamei, and haemulon scudderi. | the objective of this investigation was to assess the transfer of lead (pb) along an experimental, four-level food chain: tetraselmis suecica (phytoplankton) → artemia franciscana (crustacean, brine shrimp) → litopenaeus vannamei (crustacean, white shrimp) → haemulon scudderi (fish, grunt). t. suecica was exposed to a sublethal dose of pb in solution and then used as the base of a marine food chain. significant differences in pb concentrations were found between exposed organisms of the differen ... | 2010 | 21082317 |
| toxigenicity of enniatins from western australian fusarium species to brine shrimp (artemia franciscana). | the high prevalence (14 of 24 isolates) of enniatin-producing isolates from western australian fusarium species isolated from pasture legumes associated with sheep feed refusal and rat deaths, and the high toxicity of their crude extracts to brine shrimp (artemia franciscana) from a previous study warranted further investigation of this class of mycotoxin. crude extracts from fusarium acuminatum, fusarium avenaceum, fusarium tricinctum and fusarium sambucinum, along with enniatins a, a1, b and b ... | 2011 | 21352844 |
| biological responses of a simulated marine food chain to lead addition. | this investigation sought to assess the biological responses to pb along a simplified four-level food chain, from the primary producer, the microalgae tetraselmis suecica, grown in a control medium with < 1 µg/l of pb and exposed to a sublethal dose (20 µg/l of pb) and used as the base of a simulated food chain, through the primary-, secondary-, and tertiary-level consumers, namely, the brine shrimp, artemia franciscana; the white-leg shrimp, litopenaeus vannamei; and the grunt fish, haemulon sc ... | 2011 | 21442651 |
| influence of heat shock proteins induction in different yeast cell wall mutants on the protection against vibrio campbellii infection in gnotobiotically grown artemia franciscana (kellogg). | | 2010 | 21504084 |
| effects of salinity on growth and survival in five artemia franciscana (anostraca: artemiidae) populations from mexico pacific coast. | salinity is an important factor influencing growth and survival of aquatic organisms such as artemia, a valuable aquaculture species. this study evaluated the effects of salinity on a. franciscana populations from different water bodies in mexico's pacific coast. with this purpose, five autochthonous bisexual artemia populations were tested to assess their survival and growth values against salinities of 40, 60, 80, 100 and 120 g/l, under laboratory conditions (25 +/- 2 degrees c; ph 8-10; const ... | 2011 | 21513197 |
| priming the prophenoloxidase system of artemia franciscana by heat shock proteins protects against vibrio campbellii challenge. | like other invertebrates, the brine shrimp artemia franciscana relies solely on innate immunity, which by definition lacks adaptive characteristics, to combat against invading pathogens. one of the innate mechanisms is melanisation of bacteria mediated by the activation of the prophenoloxidase (propo) system. the 70 kda heat shock proteins (hsp70) derived from either prokaryote (escherichia coli) or eukaryote (artemia), well conserved and immune-dominant molecules, protect artemia against vibrio ... | 2011 | 21554959 |
| water-structuring technology with the molecular chaperone proteins: indicated application of the ╬▒-crystallin domains and imidazole-containing peptidomimetics in cosmetic skin care systems or dermatological therapeutic drug carrier formulations. | changes in structural proteins and hydration during aging are responsible for altered skin morphologic and mechanical properties manifested as wrinkling, sagging, loss of elasticity, and apparent dryness. impairment in protein hydration may add to the ultrastructural, mechanical, and biochemical changes in structural proteins in the aged skin. at innovative vision products, inc., we have pioneered a molecular chaperone protein-activated therapeutic or cosmetic platform to enable simultaneous ana ... | 2011 | 21663576 |
| Effects of a new photoactivatable cationic porphyrin on ciliated protozoa and branchiopod crustaceans, potential components of freshwater ecosystems polluted by pathogenic agents and their vectors. | The increasing use of photosensitized processes for disinfection of microbiologically polluted waters requires a precise definition of the factors controlling the degree of photosensitivity in target and non-target organisms. In this regard, tests with protozoa and invertebrates which have a natural habitat in such waters may be used as first screening methods for the assessment of possible hazards for the ecosystem. A new cationic porphyrin, namely meso-tri(N-methyl-pyridyl)mono(N-dodecyl-pyrid ... | 2011 | 22011789 |
| immunocytochemical studies on the naupliar nervous system of balanus improvisus (crustacea, cirripedia, thecostraca). | the nervous system of nauplii of the crustacean taxon cirripedia was analysed in the species balanus improvisus darwin, 1854 using for the first time immunocytochemical staining against serotonin, rfamide and alpha-tubulin in combination with confocal laser scanning microscopy. this approach revealed a circumoesophageal neuropil ring with nerves extending to the first and second antennae and to the mandibles, all features typical for crustacea. in addition, rfamidergic structures are present in ... | 2008 | 18555960 |
| virulence of luminescent and non-luminescent isogenic vibrios towards gnotobiotic artemia franciscana larvae and specific pathogen-free litopenaeus vannamei shrimp. | this study was conducted to test the virulence of luminescent (l) and non-luminescent (nl) isogenic strains of vibrio campbellii lmg21363, vibrio harveyi bb120 (wild type) and quorum-sensing mutant strains derived from the wild type such as vibrio harveyi bb152, bb170, mm30 and bb886. | 2009 | 19187135 |
| butyltins degradation by cunninghamella elegans and cochliobolus lunatus co-culture. | organotin compounds are ubiquitous in environment. however, biodegradation of tributyltin (tbt) and dibutyltin (dbt) to non toxic metabolites by fungi has been seldom observed. in this study we constructed a fungal co-culture with an efficient ability of tbt and its metabolites removal. the microscopic fungus strain cunninghamella elegans degraded tbt via hydroxybutyldibutyltin (ohbudbt) to its metabolites: dbt and monobutyltin (mbt), which were then transformed by cochliobolus lunatus. the sequ ... | 2012 | 23314396 |
| the small heat shock protein p26 aids development of encysting artemia embryos, prevents spontaneous diapause termination and protects against stress. | artemia franciscana embryos enter diapause as encysted gastrulae, a physiological state of metabolic dormancy and enhanced stress resistance. the objective of this study was to use rnai to investigate the function of p26, an abundant, diapause-specific small heat shock protein, in the development and behavior of encysted artemia embryos (cysts). rnai methodology was developed where injection of artemia females with dsrna specifically eliminated p26 from cysts. p26 mrna and protein knock down wer ... | 2012 | 22952748 |
| metabolic restructuring during energy-limited states: insights from artemia franciscana embryos and other animals. | many life history stages of animals that experience environmental insults enter developmental arrested states that are characterized by reduced cellular proliferation, with or without a concurrent reduction in overall metabolism. in the case of the most profound metabolic arrest reported in invertebrates, i.e., anaerobic quiescence in artemia franciscana embryos, acidification of the intracellular milieu is a major factor governing catabolic and anabolic downregulation. release of ions from intr ... | 2011 | 21335009 |
| light and transmission electron microscopy of vibrio campbellii infection in gnotobiotic artemia franciscana and protection offered by a yeast mutant with elevated cell wall glucan. | luminescent vibrios are amongst the most important pathogens in aquaculture, affecting almost all types of cultured organisms. vibrio campbellii is one of these most important pathogens. in this study, the effects of feeding mnn9 yeast cell wall mutant and wild type yeast strain were investigated in the digestive tract of brine shrimp nauplii, artemia franciscana, after experimental infection with v. campbellii (lmg 21363). gnotobiotic a. franciscana nauplii were fed daily with dead aeromonas hy ... | 2012 | 22464490 |
| marine lactobacillus pentosus h16 protects artemia franciscana from vibrio alginolyticus pathogenic effects. | vibrio alginolyticus is an opportunistic pathogen which may affect different aquatic organisms. the aim of this study was to assess the probiotic properties and the protective mode of action of lactobacillus pentosus h16 against v. alginolyticus 03/8525, through in vitro and in vivo studies using artemia franciscana (hereafter artemia). this strain showed antimicrobial activity against v. alginolyticus 03/8525 and aeromonas salmonicida subsp. salmonicida atcc33658 possibly related to lactobacill ... | 2015 | 25667335 |
| group 1 lea proteins, an ancestral plant protein group, are also present in other eukaryotes, and in the archeae and bacteria domains. | water is an essential element for living organisms, such that various responses have evolved to withstand water deficit in all living species. the study of these responses in plants has had particular relevance given the negative impact of water scarcity on agriculture. among the molecules highly associated with plant responses to water limitation are the so-called late embryogenesis abundant (lea) proteins. these proteins are ubiquitous in the plant kingdom and accumulate during the late phase ... | 2013 | 23861025 |
| characterization of the distal-less gene homologue, nldll, in the brown planthopper, nilaparvata lugens (stål). | the brown planthopper, nilaparvata lugens (stål), is a globally devastating insect pest of rice, particularly in eastern asia. distal-less or dll is a highly conserved and well studied transcription factor required for limb formation in invertebrates and vertebrates. we have identified a homologue of this gene, nldll, and demonstrated that it is expressed in all life stages of n. lugens, particularly in adult brachypterous females. when we compared between specific adult tissues it was expressed ... | 2014 | 24321689 |
| evaluation of the probiotics bacillus subtilis and lactobacillus plantarum bioencapsulated in artemia nauplii against vibriosis in european sea bass larvae (dicentrarchus labrax, l.). | two potential probiotics bacillus subtilis and lactobacillus plantarum were evaluated for use in aquaculture as preventive measures against vibriosis. in vitro evaluation of the probiotics using co-culture assays with the pathogen vibrio anguillarum and testing for the production of antibacterial substances showed the presence of antagonism and confirmed the production of antibacterial substances. both potential probiotics were administered to the live fish feed artemia franciscana nauplii, offe ... | 2012 | 22806117 |
| feeding truncated heat shock protein 70s protect artemia franciscana against virulent vibrio campbellii challenge. | the 70 kda heat shock proteins (hsp70s) are highly conserved in evolution, leading to striking similarities in structure and composition between eukaryotic hsp70s and their homologs in prokaryotes. the eukaryotic hsp70 like the dnak (escherichia coli equivalent hsp70) protein, consist of three functionally distinct domains: an n-terminal 44-kda atpase portion, an 18-kda peptide-binding domain and a c-terminal 10-kda fragment. previously, the amino acid sequence of eukaryotic (the brine shrimp ar ... | 2013 | 23092733 |
| alachlor oxidation by the filamentous fungus paecilomyces marquandii. | alachlor, a popular chloroacetanilide herbicide, can be a potential health risk factor. soil microorganisms are primarily responsible for conversion and migration of alachlor in natural environment, but knowledge concerning alachlor biodegradation is not complete. therefore, we studied the ability of paecilomyces marquandii, soil fungus tolerant to heavy metals, to eliminate alachlor and proposed a new pathway of its transformation. after 7 days of incubation only 3.3% of alachlor was detected f ... | 2013 | 23974531 |
| the suppressor of aac2 lethality sal1 modulates sensitivity of heterologously expressed artemia adp/atp carrier to bongkrekate in yeast. | the adp/atp carrier protein (aac) expressed in artemia franciscana is refractory to bongkrekate. we generated two strains of saccharomyces cerevisiae where aac1 and aac3 were inactivated and the aac2 isoform was replaced with artemia aac containing a hemagglutinin tag (araac-ha). in one of the strains the suppressor of δaac2 lethality, sal1, was also inactivated but a plasmid coding for yeast aac2 was included, because the araacδsal1δ strain was lethal. in both strains araac-ha was expressed and ... | 2013 | 24073201 |
| surface area estimation of the gut segments of artemia franciscana nauplii fed with mnn9 yeast vs. wild type yeast. | | 2013 | 25141655 |
| degradation and toxicity reduction of the endocrine disruptors nonylphenol, 4-tert-octylphenol and 4-cumylphenol by the non-ligninolytic fungus umbelopsis isabellina. | nonylphenol (np), 4-tert-octylphenol (4-t-op) and 4-cumylphenol (4-cp) are pollutants that are known as endocrine disruptors mainly due to their estrogen-mimicking activity. these phenolic substances are used in a wide range of industrial and commercial applications. in the present study, biodegradation of tnp, 4-t-op and 4-cp using the non-ligninolytic fungus umbelopsis isabellina was investigated. after 12h of incubation, more than 90% of initially applied tnp, 4-t-op and 4-cp (25mgl(-1)) were ... | 2016 | 26492175 |