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circular dichroism of squid rhodopsin and its intermediates.circular dichroism (cd) and absorption spectra of squid (todarodes pacificus) rhodopsin, isorhodopsin and the intermediates was measured at low temperatures. squid rhodopsin has positive cd bands at wavelengths corresponding the alpha- and beta-absorption bands at liquid nitrogen temperature (cd maxima: 485 nm at alpha-band and 348 nm at beta-band) as well as at room temperature (cd maxima: 474 nm at alpha-band and 347 nm at beta-band). the rotational strength of the alpha-band has a molecular e ...1978718881
cloning and nucleotide sequence of cdna for retinochrome, retinal photoisomerase from the squid retina.the rhodopsin-retinochrome system is essential for the visual photoreception of molluscs. cdna coding for retinochrome of the squid (todarodes pacificus) was cloned and the nucleotide sequence has been determined. the sequence (2.1 kb) covers the whole coding region of 903 bp. the deduced primary sequence suggests that retinochrome contains seven transmembrane spanning domains. the homology with bovine rhodopsin and the possible retinal binding site are also discussed.19902226795
calcium binding and calcium-sensitivity of heavy meromyosin and subfragment-1 from squid (todarodes pacificus) mantle and scallop (patinopecten yessoensis) adductor muscles.1. hmm and s-1 both bind one mol of calcium per mole of head, and a half of the calcium binding was diminished upon magnesium addition (10 mm) at the low affinity site. 2. the mg-atpase activity of hmm (without actin) was fully activated by the binding of one mol of calcium bound per mol of hmm. 3. the calcium binding profile to s-1 is the same as that to hmm, however, the mg-atpase activity of s-1 is independent of calcium binding. it is suggested that there are two kinds of myosin head (or s-1 ...19892523274
inhibitory effect of squid (todarodes pacificus) paramyosin on actomyosin atpase and on superprecipitation.1. paramyosin from squid mantle muscle inhibited the mg-atpase and the superprecipitation activities of actomyosin. 2. the inhibition was detected only when paramyosin forms a cofilament with myosin. 3. atp-induced changes in the morphology of the cofilament of myosin and paramyosin are involved in the inhibition by paramyosin.19882977747
irbp-like proteins in the eyes of six cephalopod species--immunochemical relationship to vertebrate interstitial retinol-binding protein (irbp) and cephalopod retinal-binding protein.sds polyacrylamide gel electrophoresis and immunoblotting were used to examine soluble proteins from the eyes of six species of cephalopods i.e. lolliguncula brevis, sepia officinalis, octopus maya, octopus bimaculoides, rossia pacifica and loligo opalescens. all species had a protein ("irbp") with molecular weight virtually identical with vertebrate interstitial retinol-binding protein (irbp) averaging 132,400 +/- 700 (n = 6). "irbp" reacted on nitrocellulose blot transfers with rabbit antibovi ...19883195063
[interaction between various cholinesterases and reversible inhibitors of polymethylene-bis-(trimethylammonium) diiodide series].it is established that derivatives of polymethylene bistrimethylammonium (ch3)3n+(ch2)nn+(ch3)3 (n = 4-10) are reversible competitive and mixed action inhibitors with respect to acetylcholinesterase of human erythrocytes, butyryl cholinesterase of horse blood serum, cholinesterase of frog brain and todarodes pacificus optical ganglion. in case of mammals and frog cholinesterase the inhibitors efficiency rises with n, but the activity of the todarodes pacificus cholinesterase less sensitive of th ...19836600861
[the conformational aspects of the interaction of the cholinesterases of pacific ocean squid and vertebrates with derivatives of polymethylene bis(trimethylammonium)].conformational properties of a series of polymethylenebis (trimethylammonium) derivatives [formula: see text] (n = 4-10), cholinesterase reversible inhibitors, were studied by molecular mechanics method. conformation-activity relationships between these inhibitors and human erythrocyte acetylcholinesterase, horse plasma butyrylcholinesterase, cholinesterases from the brain of the frog rana temporaria and from visual ganglia of the squid todarodes pacificus were investigated by correlational meth ...19947817652
larval anisakid nematodes of japanese common squid (todarodes pacificus) from the sea of japan.two species of third-stage larvae of the family anisakidae are described from the japanese common squid (todarodes pacificus steenstrup) from the sea of japan. the larvae of 1 of them, anisakis simplex (rudolphi, 1809), commonly occur encapsulated in the viscera of squid, whereas those of another 1, identified as lappetascaris sp., are found free in the mantle musculature. the latter, characterized by the presence of a very long ventricular appendix, a conspicuous esophageal gland, and the struc ...19957876981
cloning and nucleotide sequence of cdna for rhodopsin of the squid todarodes pacificus.a cdna for rhodopsin was isolated from a library constructed from poly(a)+rna of the squid (todarodes pacificus) retina. one positive clone with the longest insert of cdna (3.1 kb) was selected by employing a pcr-amplified cdna fragment as a probe. the nucleotide sequence of the cdna revealed a single open reading frame of 1,344 bp encoding a polypeptide (m(r)49,833), which covered a complete sequence for the squid opsin. this clone had a very long 3'-non-coding region (1.7 kb) including multipl ...19938428633
[the interaction of hexamethonium derivatives with the cholinesterases of vertebrates and invertebrates. the physicochemical and conformational aspects].conformational and geometrical properties of the hexamethylenebis(trimethylammonium) (ch3)3n+-(ch2)6)-n+(ch3)3 (hexamethonium) and its derivatives with various degree of conformational flexibility of interonion chain having disulfide or two dimethylsilane groups or difluoromethylene chain instead of cholinesterase reversible inhibitors have been determined using molecular mechanics methods. conformation-activity relationships between these inhibitors and different cholinesterases (human erythroc ...19958779278
[the interaction of organosilicon bisammonium compounds with the cholinesterases of far-eastern squids and vertebrates. the comparative enzymological, kinetic and conformational aspects].the research of interaction of cholinesterases from human erythrocytes, horse blood serum, brain of the frog rana temporaria, optical ganglia of the pacific squid todarodes pacificus, as well as optical ganglia of specimens of the comandor squid berryteuthis magister from different areas in the bering sea with 13 organosilicon bis (trimethylammonium) compounds (silane and siloxane rows) as reversible cholinesterase inhibitors have been first studied and generalized. calculations of the effector ...19958787143
identification of the first major allergen of a squid (todarodes pacificus).in japan, squid is an important seafood, and some patients with food allergies are sensitive to squid. there has been no report, however, describing the major allergens of squid.19968939158
[different aspects of the substrate specificity of the cholinesterase in the optical ganglia of the pacific ocean squid todarodes pacificus].it is the first time when the substrate specificity is considered as a complex phenomenon, taking into account an influence of substrate structure on the rate of enzymatic hydrolysis and interaction of cholinesterases with reversible and irreversible inhibitors. kinetic parameters of hydrolysis of 18 esters, including choline, ammonium and indophenol derivates, are presented, as affected by cholinesterase from optical ganglia of pacific squid todarodes pacificus, by acetylcholinesterase from hum ...19969054172
partial purification and characterization of six transglutaminases from ordinary muscles of various fishes and marine invertebrates.six transglutaminases were prepared from ordinary muscles of scallop (patinopecten yessoensis), botan shrimp (pandalus nipponensis), squid (todarodes pacificus), carp (cyprinus carpio), rainbow trout (oncorhynchus mykiss), and atka mackerel (pleurogrammus azonus), and their physicochemical and enzymatic properties were compared with each other. the km value of carp transglutaminase for monodansyl cadaverine, a kind of primary amines, was 0.33 mm, while the other enzymes had km values of 0.01-0.0 ...19979440224
occurrence of gangliosides in the common squid and pacific octopus among protostomia.acidic lipids from tissues of the common squid todarodes pacificus and the pacific octopus octopus vulgaris were characterized. hepatopancreatic tissues of both animals had complex compositions of resorcinol-positive acidic lipids, many of which became reactive with cholera toxin b subunit and anti-g(m1) antibody after in situ treatment with sialidase on tlc. one of the major acidic lipids in squid tissue was isolated and examined for its structure. this acidic lipid was identified to be the gan ...200111286970
amino acid sequence of squid troponin c.the complete amino acid sequence of squid todarodes pacificus troponin c (tnc), which was shown to bind only 1 mol ca(2+)/mol, was determined by both the edman and cdna methods. the squid tnc is composed of 147 amino acids including an unblocked pro at the n-terminus and the calculated molecular weight is 17003.9. among the four potential ca(2+)-binding sites, namely sites i-iv from the n-terminus, only site iv completely satisfied the consensus amino acid sequence for the active ca(2+)-binding ...200111435133
isolation and characteristics of trypsin inhibitor from the hepatopancreas of a squid (todarodes pacificus).trypsin inhibitor was purified from the hepatopancreas of squid (todarodes pacificus). the final inhibitor preparation was nearly homogeneous by sds-page with an estimated molecular weight of approximately 6300. the squid trypsin inhibitor was acid- and heat-stable, and active against trypsins from the pyloric ceca of starfish (asterias amurensis) and saury (cololabis saira) and porcine pancreatic trypsin. amino acid composition of the squid trypsin inhibitor was compared with other invertebrate ...200111470450
major and c-series gangliosides in lenticular tissues: mammals to molluscs.gangliosides of eye lenses were examined in mammals (rat, rabbits, pig, cow), bird (chicken), reptile (terrapin), amphibian (bullfrog), bony fish (red sea bream, bluefin tuna, bonito, pacific mackerel) and molluscs (common squid, pacific octopus). besides the fact that gm3 was the common ganglioside species, the composition of major gangliosides in mammalian eye lenses significantly differed from each other. while gangliotetraose gangliosides were abundant in rat eye lens, they did not constitut ...200111567893
[comparative study of catalytic properties of the liver monoamine oxidase from the squid todarodes pacificus and the wistar rat]. 200111605436
specific accumulation of polychlorinated biphenyls and organochlorine pesticides in japanese common squid as a bioindicator.organochlorines (ocs) representing persistent organic pollutants (pops) such as pcbs (polychlorinated biphenyls), ddts (ddt and its metabolites), chls (chlordane compounds), hchs (hexachlorocyclohexane isomers) and hcb (hexachlorobenzene) were determined in the liver of japanese common squid (todarodes pacificus) collected from the waters around japan (japan sea and western north pacific ocean). among ocs concentrations, pcbs (upto 5600 ng/g lipid wt.) were the highest, and those of other ocs we ...200312810316
identification of high molecular weight proteins in squid muscle by western blotting analysis and postmortem rheological changes.the high molecular weight protein connectin (also called titin) in japanese common squid (todarodes pacificus) mantle muscle was identified by western blotting analysis with 3b9, the mouse anti-chicken skeletal muscle connectin monoclonal antibody. similarly to vertebrate samples, there exists connectin in invertebrate squid mantle muscle, and the amino acid sequences are assumed to resemble those present in the a band of vertebrate connectin, judging by the specificity of 3b9. moreover, the con ...200415170118
renal organs of cephalopods: a habitat for dicyemids and chromidinids.the renal organs of 32 species of cephalopods (renal appendage of all cephalopods, and renal and pancreatic appendages in decapods) were examined for parasite fauna and for histological comparison. two phylogenetically distant organisms, dicyemid mesozoans and chromidinid ciliates, were found in 20 cephalopod species. most benthic cephalopods (octopus and cuttlefish) were infected with dicyemids. two pelagic cephalopod species, sepioteuthis lessoniana and todarodes pacificus, also harbored dicye ...200415376274
a cysteine-activated protease isolated from todarodes pacificus squid degrades collagen below its denaturation temperature.collagen purified from the mantle muscle of the japanese common squid, todarodes pacificus, showed autodegradation during incubation under acidic conditions at 25 degrees c, without the addition of exogenous enzymes. this suggests that the collagenolytic proteases bind to collagen tightly through the steps of collagen preparation. collagenolytic activity also was detected in a crude extract of mantle muscle, and leupeptin and e-64 were observed to inhibit collagenolytic activity within the colla ...200415763926
cloning and nucleotide sequence of a novel 28-kda protein from the mantle muscle of the squid todarodes pacificus with homology to tropomyosin.in recent studies, we found autodegradation of collagen from the mantle muscle of the squid todarodes pacificus and also that the 28- and 25-kda proteins are closely related to this phenomenon [connect. tissue res. 45 (2004) 109-121]. we obtained partial sequences of three internal portions of this protein, which suggested that 25-kda protein is a partially degraded form of the 28-kda protein. we determined the full cdna sequence of this protein by the degenerate polymerase chain reaction (pcr) ...200515820129
purification, cloning, and expression of an alpha/beta-galactoside alpha-2,3-sialyltransferase from a luminous marine bacterium, photobacterium phosphoreum.a novel sialyltransferase, alpha/beta-galactoside alpha-2,3-sialyltransferase, was purified from the cell lysate of a luminous marine bacterium, photobacterium phosphoreum jt-ish-467, isolated from the japanese common squid (todarodes pacificus). the gene encoding the enzyme was cloned from the genomic library of the bacterium using probes derived from the nh(2)-terminal and internal amino acid sequences. an open reading frame of 409 amino acids was identified, and the sequence had 32% identity ...200717702755
molecular and physical characteristics of squid (todarodes pacificus) skin collagens and biological properties of their enzymatic hydrolysates.the physicochemical properties of squid skin collagens and biological activity of their enzymatic hydrolysates were determined to produce more value-added materials. the amino acid compositions of the inner and outer squid skin collagens were similar, but distinct from that of bovine tendon collagen in respect to the higher levels of aspartic acid, arginine, threonine, and serine, and of the lower levels of alanine, proline, and hydroxyproline. sds-page patterns suggested that squid skin collage ...200818460118
crystal structure of squid rhodopsin.invertebrate phototransduction uses an inositol-1,4,5-trisphosphate signalling cascade in which photoactivated rhodopsin stimulates a g(q)-type g protein, that is, a class of g protein that stimulates membrane-bound phospholipase cbeta. the same cascade is used by many g-protein-coupled receptors, indicating that invertebrate rhodopsin is a prototypical member. here we report the crystal structure of squid (todarodes pacificus) rhodopsin at 2.5 a resolution. among seven transmembrane alpha-helic ...200818480818
[comparative analysis of sensitivity of cholinesterase inhibitors by multidimensional statistical methods].using the methods of factor and cluster analysis, the statistical treatment is performed of data on interaction of 7 cholinesterases (che)--human acetylcholinesterase, horse butyrylcholinesterase, cholinesterases of frog brain and of various squid species (todarodes pacificus and berrytheutis magister; in the latter case, individuals from three different habitats are compared)--with 141 reversible inhibitors of various structures. statistically significant differences between che of squids and v ...200818959211
fluctuations in abundance of common squid, todarodes pacificus in the far east.long term fluctuations in the abundance of common squid, todarodes pacificus are taken in the waters around korea and japan from 1952 to 2003. the year-to-year fluctuations in the abundance in the tsushima warm current (twc) region (yellow sea- east china sea-east/japan sea) revealed the same pattern with that in the kuroshio-oyashio current (koc) region (northern pacific) except for the period of fishing shifts from the koc to twc region in the late 1960s and 1970s. two period of high abundance ...200819195378
[comparative-enzymological study of cholinesterases from optic ganglia of the commander squid berryteuthis magister individuals inhabiting different zones of the species areal].in this review a comparative analysis is performed of enzymological characteristics of cholinesterase (che) from optic ganglia of individuals of the commander squid berryteuthis magister caught in 8 zones of its habitation areal in the northern-western pacific aquatorium, of che of the pacific squid todarodes pacificus as well as of the "standard" acetylcholinesterase from human erythrocytes and butyrylcholinesterase from horse blood serum. by the method of the substrate-inhibitor analysis there ...201021061644
[how the various substrates activate the process of enzymatic hydrolysis by different cholinesterases].kinetic analysis of the activating effect of substrate on the cholinesterase catalysis is performed. there are determined values of coefficient of activation a in the ph zone 5.0-7.5 for the process of hydrolysis of acetylcholine, indophenylacetate (ipa), and 2,6-dichlorophenolindophenylacetate (dipa) by cholinesterase (che) of horse blood serum, as well as of ipa and dipa by che of optical ganglia of the pacific squid todarodes pacificus. the phenomenon of activation has not been revealed at hy ...201021268878
[siliconorganic reversible inhibitors of cholinesterases of various animals].the review present data on cholinesterase effects of 28 specially synthesized siliconorganic compounds (monoonium, clementorganic, and bisonium derivatives) studied as reversible inhibitors of acetylcholinesterase (acetyl-che) of human erythrocytes, butyryl-che of horse blood serum, che of brain of common frog rana temporaria, che of the optical ganglia tissue of pacific squid todarodes pacificus and of individuals of commandor squid berryteuthis magister from various habitats in the northwester ...201121598695
transcriptome analysis of the duodenum in wistar rats fed a trypsin inhibitor derived from squid viscera.to investigate the effects of oral administration of a trypsin inhibitor (ti), normal wistar rats were fed a ti derived from squid ( todarodes pacificus ) for 10 weeks and gene expression profiles in the duodenum, pancreas, liver, and muscle were then analyzed using dna microarrays. although no significant changes could be observed in growth, food intake, tissue weight, or blood tests among the tissues tested, the duodenum showed the most remarkable changes in the global gene expression profile. ...201121761937
thermodynamic characterization of muscle tropomyosins from marine invertebrates.structural properties of invertebrate muscle tropomyosin (tm) have not been characterized in detail to date. tms were thus purified from the mantle muscle of japanese common squid todarodes pacificus, the foot muscle of tokobushi abalone haliotis diversicolor and the tail muscle of kuruma prawn marsupenaeus japonicus, and investigated for their thermodynamic properties by circular dichroism (cd) spectrometry and differential scanning calorimetry (dsc). from the cd spectrometry data, the apparent ...201121722747
Quantum mechanical/molecular mechanical structure, enantioselectivity, and spectroscopy of hydroxyretinals and insights into the evolution of color vision in small white butterflies.Since Vogt's discovery of A(3)-retinal or 3-hydroxyretinal in insects in 1983 and Matsui's discovery of A(4)-retinal or 4-hydroxyretinal in firefly squid in 1988, hydroxyretinal-protein interactions mediating vision have remained largely unexplored. In the present study, A(3)- and A(4)-retinals are theoretically incorporated into squid and bovine visual pigments by use of the hybrid quantum mechanics/molecular mechanics [SORCI+Q//B3LYP/6-31G(d):Amber96] method, and insights into structure, enant ...201122087641
cadmium-binding proteins of three marine molluscs and characterization of two cadmium-binding glycoproteins from the hepatopancreas of a whelk, buccinum tenuissimum.the cadmium-binding proteins were shown to exist in the hepatopancreas of three molluscs, a whelk, buccinum tenuissimum, a turbo, batillus cornutus, and a squid, todarodes pacificus. cadmium was efficiently accumulated in nature to a mean concentration of 119, 33, and 50 micrograms/g wet tissue in the hepatopancreas of three species of molluscs, and 30%, 11%, and 43% of the element in each tissue of whelk, turbo, and squid was extracted to the soluble fraction, respectively. separation of the so ...20133709465
effect of uv-c light on the microbial and sensory quality of seasoned dried seafood.this study investigated the effects of different doses of uv-c light at 253.7 nm (0-18 kj/m(2)) on the reduction of escherichia coli,staphylococcus aureus and bacillus cereus in contaminated seasoned dried filefish (thamnaconus modestus) and sliced squid (todarodes pacificus) surfaces and sensory quality. the counts of all three bacteria were significantly (p < 0.05) reduced by the increase of uv-c dosage.e. coli,s. aureus and b. cereus on filefish with 18 kj/m(2)of uv-c maximally reduced by 2.7 ...201625967394
the functional-morphological adaptive strategy of digestive organs of decapodiform cephalopods.the digestive organs in decapodiform cephalopod species morphologically vary by individual lifestyle. we examined the following six species of adult decapodiformes cephalopods representing different habitats: todarodes pacificus, loligo bleekeri, loligo edulis, watasenia scintillans (pelagic), sepia lycidas and euprymna morsei (benthic). l. bleekeri and l. edulis possess a bursiform cecal sac connected to the cecum. pelagic species have a single digestive gland smaller than in benthic species. t ...201626369293
sedimentitalea todarodis sp. nov., isolated from the intestinal tract of a japanese flying squid.a novel gram-stain-negative, motile, aerobic and rod-shaped alphaproteobacterium, designated strain khs03t, was isolated from the intestinal tract of a japanese flying squid, todarodes pacificus, which was collected from the east sea, korea. the new isolate shared 97.4 % 16s rrna gene sequence similarity with sedimentitalea nanhaiensis nh52ft. the isolate grew optimally at 25 °c and ph 7 in the presence of 1-2 % (w/v) nacl, and had an absolute requirement of sodium ions for growth. the major cel ...201627226032
pseudahrensia todarodis sp. nov., isolated from the gut of a japanese flying squid, todarodes pacificus.a novel gram-stain-negative, non-motile, non-spore-forming, non-flagellated, aerobic, beige-coloured and rod-shaped bacterium, designated strain khs02t, was isolated from the intestinal tract of a japanese flying squid, todarodes pacificus, which was collected from the east sea, korea. phylogenetic analysis of 16s rrna gene sequences revealed that strain khs02t formed a monophyletic clade with p. aquimaris hdw-32t, with which it had the highest sequence similarity (98.67 %). strain khs02t grew o ...201626763785
[hysterothylacium petteri sp. n. (nematoda: ascaridata) from the pacific ocean swordfish].hysterothylacium petteri sp. n. was recovered from the stomach of xiphias gladius occurring in the kuroshio. the morphological difference of h. petteri from all other species of this genus is the extremely long ventricular caecum (two times more than pharynx). the species is very similar to h. corrugatum deardorff et overstreet, 1980 from swordfish off the american shores and corresponds to the description of hysterothylacium sp. by petter, maillard, 1987. the third stage larvae of h. petteri sp ...20071792100
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