| amino acid sequence of the dimeric hemoglobin (hb i) from the deep-sea cold-seep clam calyptogena soyoae and the phylogenetic relationship with other molluscan globins. | the deep-sea cold-seep clam calyptogena soyoae has two homodimeric hemoglobins (hbs i and ii) in erythrocytes. the complete amino acid sequence of hb i has been determined. it is composed of 144 amino acid residues, has a high content of hydrophobic residues, and a calculated molecular weight of 16,350 including a heme group. the sequence of calyptogena hb i showed high homology (42% identity) with that of calyptogena hb ii (suzuki, t., takagi t. and ohta, s. (1989) biochem. j. 260, 177-182), al ... | 1989 | 2690960 |
| characterization of the endosymbiont of a deep-sea bivalve, calyptogena soyoae. | we have purified dna from gill tissue of a marine bivalve, calyptogena soyoae, collected from the deep-sea cold seep communities in sagami bay, japan. an rrna gene was amplified, cloned, and sequenced. in situ hybridization revealed that the sequence is that of a bacterial endosymbiont within the gill of c. soyoae. | 1995 | 7574622 |
| isolation and cdna-derived amino acid sequences of hemoglobin and myoglobin from the deep-sea clam calyptogena kaikoi. | the heterodont clam calyptogena kaikoi, living in the cold-seep area at a depth of 3761 m of the nankai trough, japan, has abundant hemoglobins and myoglobins in erythrocytes and adductor muscle, respectively. two types of hemoglobins (hb i and hb ii) were isolated, and the complete amino acid sequences of hb i (145 residues) and hb ii (137 residues) were obtained with combination of cdna and protein sequencing. the amino acid sequences of c. kaikoi hbs i and ii differed from homologous chains o ... | 2000 | 10719183 |
| the hemoglobin gene of the deep-sea clam calyptogena soyoae has a novel intron in a-helix. | the cdnas encoding two dimeric hemoglobins, hbs i and ii, of the deep-sea clam calyptogena soyoae were amplified by pcr and the complete nucleotide sequences determined. the cdna-derived amino acid sequences agreed completely with those determined chemically. many of the molluscan intracellular globin genes have a characteristic four-exon/three-intron structure, with the precoding and two conventional introns conserved widely in animal globin genes. in this work we have determined the exon/intro ... | 2005 | 11137460 |
| a novel alveolate in bivalves with chemosynthetic bacteria inhabiting deep-sea methane seeps. | it has recently been unveiled that a wide variety of microbial eukaryotes (protists) occur in chemosynthetic ecosystems, such as hydrothermal vents and methane seeps. however, there is little knowledge regarding protists associated with endemic animals inhabiting these environments. in the present study, utilizing pcr techniques, we detected fragments of the small subunit ribosomal rna gene (ssu rrna gene) from a particular protist from gill tissues of a significant fraction of the vesicomyid cl ... | 2013 | 23316697 |
| biosurfactant-producing yeast isolated from calyptogena soyoae (deep-sea cold-seep clam) in the deep sea. | we describe a detailed structure determination of biosurfactant produced by pseudozyma hubeiensis sy62, which was newly isolated from calyptogena soyoae (deep-sea cold-seep clam, shirouri-gai) at 1156 m in sagami bay. p. hubeiensis sy62 was taxonomically slightly different from the p. hubeiensis type strain, which produces biosurfactants. glycolipid production by the strain was also slightly different from those of previously reported strains. bs productivity was estimated to be around 30 g/l fr ... | 2010 | 20547357 |
| tissue culture of the deep-sea bivalve calyptogena soyoae. | tissue culture for the deep-sea clam calyptogena soyoae (c. soyoae) has been examined. mantle tissue was cultured in dulbecco's modified eagle medium that was prepared using artificial seawater supplemented with fetal bovine serum (fbs) and the body fluid of c. soyoae. the mantle cells were viable in culture for at least 13 days at 4 degrees c and atmospheric pressure on a polylysine-coated dish, although no cells attached in the body fluid-free culture medium. it was found that mantle cells syn ... | 2000 | 11139082 |
| primary structure of a dimeric haemoglobin from the deep-sea cold-seep clam calyptogena soyoae. | the heterodont clam calyptogena soyoae, living in the cold-seep area of the upper bathyal depth of sagami bay, japan, has two homodimeric haemoglobins (hb i and hb ii) in erythrocytes. the complete amino acid sequence of 136 residues of c. soyoae hb ii was determined. the sequence showed low homology with any other globins (at most 20% identity) and lacked the n-terminal extension of seven to nine amino acid residues characteristic of all the molluscan haemoglobins sequenced hitherto. although t ... | 1989 | 2775180 |