| isolation, characterization and immunocytochemical localization of caldesmon-like protein from molluscan striated muscle. | a 140-kda polypeptide present in the striated muscle of pecten maximus and sepia officinalis was purified to homogeneity and its main properties were investigated using biochemical and cytochemical approaches. the protein was found to be similar to chicken gizzard caldesmon. it is a heat-stable protein. it cross-reacts immunologically with anti-(gizzard caldesmon) antibody, binds to calmodulin-sepharose in a ca2+-dependent manner, cosediments with f-actin filaments and acts in the absence and pr ... | 1989 | 2531664 |
| patterns of experimental contamination by protogonyaulax tamarensis in some french commercial shellfish. | as a result of the proliferation of toxic marine dinoflagellates along european coasts and the recent discovery of paralytic poisons in french shellfish, experimental studies were conducted on four species of shellfish from the brittany coasts. contamination rates of a culture of toxic protogonyaulax tamarensis, were determined for mytilus edulis, crassostrea gigas, pecten maximus and ruditapes philippinarum. mussels and scallops were very rapidly contaminated showing high toxin accumulation rat ... | 1989 | 2629173 |
| the subunits and biological activity of polymorphic forms of tropomyosin. | 1. free thiol groups were shown to be essential for tropomyosin to effect maximum inhibition of the ca(2+)-stimulated atpase (adenosine triphosphatase) of desensitized actomyosin but not for its activity in the regulatory-protein system. 2. the activity of tropomyosin on the mg(2+)-stimulated atpase in the regulatory-protein system was more susceptible to enzymic digestion and thermal denaturation than its effect on the ca(2+)-stimulated atpase of actomyosin. 3. rabbit skeletal tropomyosin migra ... | 1973 | 4270662 |
| characterization of binding sites for calcitonin gene-related peptide in the mollusc gill. | target organs for calcitonin gene-related peptide (cgrp) were investigated in pecten maximus using 125i-labelled human cgrp. cgrp was shown to interact specifically with mantle and gill tissue. receptor studies using branchial membrane preparations indicated that the binding was time dependent. scatchard analysis of binding data showed that there was a single class of binding sites. the affinity constant was found to be 0.7.10(8) m-1 and the number of binding sites 2600.10(8)/mg of protein. salm ... | 1996 | 8701028 |
| [international trade in bivalve mollusks and the current situation in france and in europe]. | over the last century and a half, trade in shellfish has introduced into france four new species intended for aquaculture, as follows: -the portuguese oyster (crassostrea angulata) -the american clam (mercenaria mercenaria) -the pacific oyster (c. gigas) -the manila clam (ruditapes philippinarum). the development of hatcheries and air transport facilities has led to increased trade in these species among the countries of the european union (france, ireland, italy, the netherlands, portugal, spai ... | 1996 | 9019238 |
| comparison of enzyme immunoassay and mouse bioassay for determining paralytic shellfish poisoning toxins in shellfish. | sixty shellfish samples (mussel [mytilus edulis], queen scallops [chlamys opercularis] and king scallops [pecten maximus]), collected during routine surveillance for paralytic shellfish poisoning (psp) toxins conducted by the uk ministry of agriculture, fisheries and food and the scottish office agriculture, environmental fisheries department, were analysed for contamination with psp toxins by mouse bioassay (mba), and by a competitive direct enzyme immunoassay (ridascreen saxitoxin elisa). usin ... | 1997 | 9102352 |
| amylase on pecten maximus (mollusca, bivalves): protein and cdna characterization; quantification of the expression in the digestive gland. | the digestive enzyme alpha-amylase in pecten maximus has been purified from the digestive gland, where it is present as two isoforms, in order to gain information on its structure and regulation, a digestive gland cdna library, constructed in lambda phage zap ii (stratagene, la jolla, calif., u.s.a.), was screened with a shrimp alpha-amylase cdna probe. only 0.02% of the clones were positive, and the longest clone, having a size of 1700 bp and identical to that of the mrna, was fully sequenced. ... | 1997 | 9284561 |
| specific inhibition of chemiluminescent activity by pathogenic vibrios in hemocytes of two marine bivalves: pecten maximus and crassostrea gigas. | hemocytes from two adult bivalves, pecten maximus and crassostrea gigas, were exposed to 12 different bacterial strains including 2 alteromonas spp. (u1 and t413), 2 type strains of vibrios (v. anguillarum atcc 19264 and v. alginolyticus atcc 17749), 1 vibrio (s322) pathogenic to c. gigas larvae, 2 vibrios (v110 and v117) virulent to ostrea edulis larvae, and 5 different strains of a same vibrio sp. (group a496) isolated from moribund p. maximus larvae. after 1.5 h contact with bacteria, zymosan ... | 1998 | 9446738 |
| stability of an infectious pancreatic necrosis virus (ipnv) isolate stored under different laboratory conditions. | an infectious pancreatic necrosis virus (ipnv) belonging to the sp serotype, isolated from scallops pecten maximus, was propagated and suspended in sterile water and cell culture medium with different salinities and incubated at temperatures ranging from -80 to 40 degrees c. virus stability was examined by measuring virus titers under different storage conditions. virus titers were also measured after repeated freezing and thawing, and in incubated sterile filtered scallop hepatopancreas, haemol ... | 1998 | 9678969 |
| quinolones from a bacterium and tyrosine metabolites from its host sponge, suberea creba from the coral sea. | a marine bacterium, identified as a pseudomonad, isolated from suberea creba bergquist, 1995 (porifera, dictyoceratida, verongida, aplysinellidae) collected along the eastern coast of new caledonia, gave in culture phenazine-alpha-carboxamide, 2-n-heptylquinol-4-one, 2-n-nonylquinol-4-one, 2-n-(1'e-nonenyl)quinol-4-one, 3-n-heptyl-3-hydroxyquinolin-2,4-dione, a n-oxide-2-n-heptylquinoline derivative, and a benzyldiketopiperazine. none of these products could be detected, at the hplc-uv sensitivi ... | 1998 | 9701634 |
| vibrio pectenicida sp. nov., a pathogen of scallop (pecten maximus) larvae. | five strains were isolated from moribund scallop (pecten maximus) larvae over 5 years (1990-1995) during outbreaks of disease in a hatchery (argenton, brittany, france). their pathogenic activity on scallop larvae was previously demonstrated by experimental exposure. the phenotypic and genotypic features of the strains were identical. the g + c content of the strains was in the range 39-41 mol%. dna-dna hybridization showed a minimum of 73% intragroup relatedness. phylogenetic analysis of small- ... | 1998 | 9731288 |
| roseobacter gallaeciensis sp. nov., a new marine bacterium isolated from rearings and collectors of the scallop pecten maximus. | four bacterial strains were isolated from larval cultures and collectors of the scallop pecten maximus. they showed a high level of intragroup genomic relatedness (84-95%) as determined by dna-dna hybridization. the cells were gram-negative, strictly aerobic, motile, ovoid rods. they grew at temperatures from 15 to 37 degrees c and from ph 7.0 to 10, but did not grow in the absence of nacl and required growth factors. they had the ability to use a wide variety of compounds as sole carbon source: ... | 1998 | 9731295 |
| amylase mrna expression in crassostrea gigas during feeding cycles. | a crassostrea gigas digestive gland copy dna (cdna) library constructed in the lambda phage zapii (stratagene, la jola, usa) was screened with an amylase heterologous proble. to get access to the complete cdna, a polymerase chain reaction extension was conducted using dna extracted from the phages. the complete cdna sequence is 1688 base pairs (embl = y08370). the deduced protein sequence is 519 aminoacids long with a 19 aminoacid signal peptide. similarity with pecten maximus amylase is 72%. a ... | 2000 | 10707321 |
| [role of biotic interactions on the growth of pre-recruitment and development of pecten maximus (l.) in the bay of breast]. | biotic interactions within benthic megafauna were studied from quantitative dredge samples to investigate their role in the persistence of low scallop, pecten maximus, stocks in the bay of brest. the distributions of 148 species of the megafauna and of four age classes of pecten maximus were sampled. suspension feeders dominate all megabenthic assemblages within the bay. this domination can be related to crepidula fornicata spreading in the bay; however, no direct competition for food between sc ... | 2000 | 11072626 |
| response of haemocyte lysosomes to bacterial inoculation in the oysters ostrea edulis l. and crassostrea gigas (thunberg) and the scallop pecten maximus (l). | data are presented that demonstrate the application of the neutral red retention assay (nrr) to monitor the effects of a bacterial inoculation on the haemocyte lysosomes of the european flat oyster ostrea edulis, pacific oyster crassostrea gigas and scallop pecten maximus. bivalves were acclimated to three temperature regimes (5, 15 and 25 degrees c), at constant salinity for 7 days in the laboratory. once baseline responses to acclimation temperature had been established, the effects of an in v ... | 2001 | 11308076 |
| toxicity to bivalve hemocytes of pathogenic vibrio cytoplasmic extract. | using a chemiluminescence (cl) test, it had been previously demonstrated that vibrio pectenicida, which is pathogenic to pecten maximus larvae, was able to inhibit completely the cl activity of p. maximus hemocytes and partially inhibit those of crassostrea gigas. conversely, a vibrio sp. strain, s322, pathogenic to c. gigas larvae was more active in reducing the cl activity of oyster hemocytes than of scallop hemocytes. using this same cl biotest, v. pectenicida and s322 cytoplasmic extracts we ... | 2001 | 11356051 |
| variations in food intake of pecten maximus (l.) from the bay of brest (france): influence of environmental factors and phytoplankton species composition. | previous studies carried out in the bay of brest on daily shell growth of pecten maximus have demonstrated that temperature is a major control on daily shell growth in contrast to food supply. however, repeated events of slow growth have been observed during diatom and dinoflagellate blooms. the aim of this study was to determine how fluctuations in environmental parameters influence p. maximus food intake and daily shell growth rate. in 1995, p. maximus food intake and growth were highest when ... | 2001 | 11510420 |
| determination of domoic acid in shellfish by liquid chromatography with electrospray ionization and multiple tandem mass spectrometry. | amnesic shellfish poisoning is a potentially lethal human toxic syndrome which is caused by domoic acid (da) that originates in marine phytoplankton belonging to the pseudonitzschia genus. a new sensitive liquid chromatographic-mass spectrometry (lc-ms) method has been developed for the determination of da in various marine biological samples. the characteristic fragmentation pathways for da were established using multiple stage ms on selected daughter ions, which were sequentially trapped and f ... | 2001 | 11771836 |
| french scallops: a new host for ostreid herpesvirus-1. | sporadic high mortalities were reported among larval french scallops (pecten maximus). electron microscopy of moribund larvae revealed particles with the characteristics of a herpesvirus in association with cellular lesions. pcr and dna sequencing showed that the virus is a variant of ostreid herpesvirus-1 that has already been described in clams and oysters. this is the first description of a herpesvirus infection of a scallop species. the virus was transmitted successfully from an extract of i ... | 2001 | 11883198 |
| food safety implications of the distribution of azaspiracids in the tissue compartments of scallops (pecten maximus). | azaspiracids, a new class of shellfish toxins, have been implicated in several recent incidents of human intoxications following the consumption of mussels (mytilus edulis). a study was undertaken to examine the distribution of azaspiracid poisoning (azp) toxins in scallops (pecten maximus) and individual shellfish were dissected into five tissue fractions for the determination of toxin composition. separation of the predominant azaspiracids, aza1-3, was achieved using reversed-phase liquid chro ... | 2003 | 12623664 |
| sterol composition of gonad, muscle and digestive gland of pecten maximus from málaga (south spain). | sterol composition and content and their seasonal variations over 18 months were investigated in adductor muscle, digestive gland and gonads of pecten maximus. sterols were isolated by silicagel 60 thin layer chromatography and identified by gas chromatography/mass spectrometry. eleven sterols were identified, with cholesterol, brassicasterol, 24-methylenecholesterol and 22-trans-dehydrocholesterol being the principal components. the same sterols were found in all three tissues independent of se ... | 2003 | 12628375 |
| the first identification of azaspiracids in shellfish from france and spain. | incidents of human intoxications throughout europe, following the consumption of mussels have been attributed to azaspiracid poisoning (azp). although first discovered in ireland, the search for the causative toxins, named azaspiracids, in other european countries has now led to the first discovery of these toxins in shellfish from france and spain. separation of the toxins, azaspiracid (aza1) and analogues, aza2 and aza3, was achieved using isocratic reversed-phase liquid chromatography coupled ... | 2003 | 12893067 |
| geographical, temporal, and species variation of the polyether toxins, azaspiracids, in shellfish. | azaspiracid poisoning (azp) is a new toxic syndrome that has caused human intoxications throughout europe following the consumption of mussels (mytilus edulis), harvested in ireland. shellfish intoxication is a consequence of toxin-bearing microalgae in the shellfish food chain, and these studies demonstrated a wide geographic distribution of toxic mussels along the entire western coastal region of ireland. the first identification of azaspiracids in other bivalve mollusks including oysters (cra ... | 2003 | 12901653 |
| demonstration of nutrient pathway from the digestive system to oocytes in the gonad intestinal loop of the scallop pecten maximus l. | the mechanism of nutrient transfer from the digestive system to the gonad acini and developing oocytes was investigated in the gonad-intestinal loop system of the queen scallop pecten maximus l. ferritin was injected directly into the purged intestine of specimens from the wild. subsequently, a histochemical reaction and transmission electron microscopy were used to localize ferritin in various cell types. ferritin was rapidly absorbed by the intestinal epithelium, and then appeared in hemocytes ... | 2003 | 12917225 |
| dd-diketopiperazines: antibiotics active against vibrio anguillarum isolated from marine bacteria associated with cultures of pecten maximus. | bacterial strains cf-20 (cect5719) and c-148 (cect5718), isolated from cultures of larvae of molluscs, are shown to produce substances 1-5 with strong antibiotic activity against vibrio anguillarum (mic: 0.03-0.07 mug/ml) and identified as the dd-diketopiperazines cyclo(d)-pro-(d)-phe (1), cyclo(d)-pro-(d)-leu (2), cyclo(d)-pro-(d)-val (3), cyclo(d)-pro-(d)-ile (4), and cyclo-trans-4-oh-(d)-pro-(d)-phe (5). comparison with other stereoisomers indicates that inhibition of v. anguillarum is associ ... | 2003 | 14575426 |
| high-energy phosphate metabolism during exercise and recovery in temperate and antarctic scallops: an in vivo 31p-nmr study. | in vivo (31)p-nuclear magnetic resonance (nmr) spectroscopy was used to measure the levels of atp, phospho-l-arginine (pla), and inorganic phosphate in the adductor muscle of the antarctic scallop adamussium colbecki and two temperate species, aequipecten opercularis and pecten maximus. graded exercise regimes from light (one to two contractions) to exhausting (failing to respond to further stimulation) were imposed on animals of each species at its habitat temperature (0 degrees vs. 12 degrees ... | 2003 | 14671710 |
| complementary dna cloning and molecular evolution of opine dehydrogenases in some marine invertebrates. | the complete complementary dna sequences of genes presumably coding for opine dehydrogenases from arabella iricolor (sandworm), haliotis discus hannai (abalone), and patinopecten yessoensis (scallop) were determined, and partial cdna sequences were derived for meretrix lusoria (japanese hard clam) and spisula sachalinensis (sakhalin surf clam). the primers odh-9f and odh-11r proved useful for amplifying the sequences for opine dehydrogenases from the 4 mollusk species investigated in this study. ... | 2004 | 15565272 |
| proteomic approach to probe for larval proteins of the mussel mytilus galloprovincialis. | a proteomic approach was used to search for larval proteins specific to the mussel mytilus galloprovincialis from galicia in northwest spain. the study included both a comparative analysis, through two-dimensional electrophoresis, of protein expression maps of the larvae of the mussel and of 5 abundant and commercially important bivalve species from the region (ostrea edulis, cerastoderma edule, pecten maximus, tapes decussatus, and venarupis pullastra) and subsequent mass spectrometric analysis ... | 2005 | 15976943 |
| identification of mytilus spp. and pecten maximus in irish waters by standard pcr of the 18s rdna gene and multiplex pcr of the 16s rdna gene. | two molecular protocols for the identification of mussel and scallop have been developed using specific primers targeting the mitochondrial 16s ribosomal dna gene and the nuclear 18s ribosomal dna gene. primers for the mitochondrial 16s ribosomal dna gene in multiplex polymerase chain reaction (pcr) protocols yielded diagnostic dna fragments for the mussels mytilus edulis, mytilus galloprovincialis, and the hybrid mytilus edulis/galloprovincialis (335 bp), the king scallop pecten maximus (382 bp ... | 2005 | 16206017 |
| amnesic shellfish poisoning toxins in bivalve molluscs in ireland. | in december 1999, domoic acid (da) a potent neurotoxin, responsible for the syndrome amnesic shellfish poisoning (asp) was detected for the first time in shellfish harvested in ireland. two liquid chromatography (lc) methods were applied to quantify da in shellfish after sample clean-up using solid-phase extraction (spe) with strong anion exchange (sax) cartridges. toxin detection was achieved using photodiode array ultraviolet (lc-uv) and multiple tandem mass spectrometry (lc-ms(n)). da was ide ... | 2005 | 16289180 |
| immunohistochemistry of great scallop pecten maximus larvae experimentally challenged with pathogenic bacteria. | three challenge experiments were carried out on larvae of the great scallop pecten maximus. larvae were bath-challenged with vibrio pectenicida and 5 strains resembling vibrio splendidus and one pseudoalteromonas sp. unchallenged larvae were used as negative controls. the challenge protocol was based on the use of a multidish system, where the scallop larvae (10, 13 and 15 d post-hatching in the 3 experiments, respectively) were distributed to 2 ml wells with stagnant seawater and exposed to the ... | 2006 | 16724560 |
| exposure to domoic acid affects larval development of king scallop pecten maximus (linnaeus, 1758). | domoic acid (da) is a highly toxic phycotoxin produced by bloom forming marine diatoms pseudo-nitzschia spp. bivalves can accumulate this toxin to a high level through their feeding activities, and thus illness or death in can occur in consumers of bivalves. in this study, king scallop, pecten maximus, larvae were exposed to dissolved domoic acid (da) for 25d, and the toxin accumulation and effects of harbouring this toxin were investigated. scallop larvae incorporated da continuously during the ... | 2007 | 17178425 |
| putative reaction mechanism of heterologously expressed octopine dehydrogenase from the great scallop, pecten maximus (l). | cdna for octopine dehydrogenase (odh) from the adductor muscle of the great scallop, pecten maximus, was cloned using 5'- and 3'-race. the cdna comprises an orf of 1197 nucleotides and the deduced amino acid sequence encodes a protein of 399 amino acids. odh was heterologously expressed in escherichia coli with a c-terminal penta his-tag. odh-5his was purified to homogeneity using metal-chelate affinity chromatography and sephadex g-100 gel filtration. recombinant odh had kinetic properties simi ... | 2007 | 18028427 |
| are spirolides converted in biological systems?--a study. | spirolides are biologically active macrocycles isolated first from scallops and phytoplankton from aquaculture sites in nova scotia, canada. these compounds displayed "fast-acting" toxicity in the traditional bioassay. that phenomenon is related to the presence of a cyclic imine function in these compounds. spirolides containing vicinal methyl groups in their seven-membered ring are suspected of being resistant to hydrolysis. we studied possible conversions of vicinal methyl groups wearing spiro ... | 2008 | 18262215 |
| organ distribution and food safety aspects of cadmium and lead in great scallops, pecten maximus l., and horse mussels, modiolus modiolus l., from norwegian waters. | the purpose of the study was to determine the levels and organ distribution of the potentially harmful inorganic elements cadmium and lead in great scallops and horse mussels from unpolluted norwegian waters. the scallops far exceeded the eu-limit for cadmium in bivalves when all soft tissues were analysed. when only muscle and gonad were included, however, the level of cadmium was acceptable, because cadmium accumulated in the digestive gland with a mean of 52 mg/kg ww (wet weight). in horse mu ... | 2008 | 18311528 |
| high-frequency archives of manganese inputs to coastal waters (bay of seine, france) resolved by the la-icp-ms analysis of calcitic growth layers along scallop shells (pecten maximus). | during their growth, bivalves are recognized to archive minor and trace elements within their shells which may reflect environmental conditions at the sediment-water interface (swi). shells from juvenile great scallops (pecten maximus (l.)), which develop a daily calcite growth layer, were collected in the bay of seine (france) and examined by matrix-matched laserablation icp-ms analysis for mn concentrations along their growth period, from april to october (year 2004). the backdated mn concentr ... | 2008 | 18350880 |
| the amylase-secreting cells of the stomach of the scallop, pecten maximus: ultrastructural, immunohistochemical and immunocytochemical characterizations. | (1) alpha-amylase was extracted and purified from the stomach/digestive gland complex of the scallop pecten maximus and an anti-serum was induced against the purified amylase by rabbit immunization. (2) the anti scallop amylase was used to localize the amylase-secreting cells in the stomach of pecten maximus by immunofluorescence and immunogold labelling. the amylase-secreting cells are glandular cells particularly numerous in the main sorting area of the stomach. their secretory granules were f ... | 1993 | 18621245 |
| bioaccumulation and detoxification processes of hg in the king scallop pecten maximus: field and laboratory investigations. | hg bioaccumulation was investigated in the king scallop pecten maximus in the laboratory and in the field. in controlled conditions, scallops were exposed to (203)hg through seawater, sediment and food in order to determine its uptake and depuration kinetics. in the field, hg and metallothionein (mt) concentrations and the metal subcellular distribution were determined in scallops from two sites of the bay of seine (france) differently subjected to the seine river inputs. while hg concentrations ... | 2008 | 18937984 |
| anatomical distribution of heavy metals in the scallop pecten maximus. | this paper studied the anatomical distribution of mercury (hg), cadmium (cd), lead (pb), chromium (cr), nickel (ni), arsenic (as), silver (ag), copper (cu) and zinc (zn) in the scallop pecten maximus and the possible implications in terms of shellfish management. six organs were analysed: mantle, gills, foot, digestive gland, kidney and gonad. on the basis of their anatomical distribution, two groups of metals were able to be distinguished: the first included pb, hg, ni, zn and ag; and the secon ... | 2008 | 19680841 |
| brominated dioxins (pbdd/fs) and pbdes in marine shellfish in the uk. | the occurrence of brominated dioxins (pbdd/fs) and polybrominated diphenyl ethers (pbdes) was investigated in commonly consumed species of marine shellfish in the uk. individual samples of pacific oysters (crassostrea gigas), native oysters (ostrea edulis), mussels (mytilus edulis), scallops (pecten maximus), and cockles (cerastoderma edule) were collected from different coastal regions between 2006 and 2007. samples of a particular species from each site were composited and 60 samples were anal ... | 2009 | 19680967 |
| distribution and linkage of domoic acid (amnesic shellfish poisoning toxins) in subcellular fractions of the digestive gland of the scallop pecten maximus. | the king scallop pecten maximus accumulates domoic acid, the main amnesic shellfish poisoning toxin, in the digestive gland for a long time. to try to find if the cause of this characteristic is the binding of the toxin to some cellular component, the subcellular distribution of domoic acid in the cells of the digestive gland was studied, by means of serial centrifugation, ultrafiltration and size exclusion chromatography (sec). domoic acid was found to be present mostly in soluble form in the c ... | 2010 | 19852975 |
| functional immune response in pecten maximus: combined effects of a pathogen-associated molecular pattern and pah exposure. | pathogen-associated molecular patterns (pamps) enable recognition of structures present in microorganisms such as lipopolysaccharides (lps). lps are an essential constituent of the outer membrane of gram-negative bacteria, stimulating the innate immune system of invertebrates. here, lps from escherichia coli (055:b5) were used to investigate the functional immune response of pecten maximus after stimulation with a pamp and to determine the combined effect of a phenanthrene exposure and lps chall ... | 2010 | 19853039 |
| ecological meltdown in the firth of clyde, scotland: two centuries of change in a coastal marine ecosystem. | the firth of clyde is a large inlet of the sea that extends over 100 km into scotland's west coast. | 2010 | 20686614 |
| hydrosoluble antioxidants by enzymatic glucosylation of a vitamin e derivative using marine α-d-glucosidase from aplysia fasciata. | relevant examples of polyglycosylating exo-glycosidases were reported among enzymes of marine origin (aplysia fasciata, geobacillus, and pecten maximus). herein we describe the enzymatic polyglucosylation of a chromane-methanol (2-hydroxymethyl-2,5,7,8-tetramethylchroman-6-ol) performed by using the α-d-glucosidase from the sea hare aplysia fasciata. new di-, tri-, and tetrasaccharide derivatives were synthesized and their antioxidant activities were evaluated by dpph test. high enzymatic substr ... | 2011 | 21210171 |
| identification of octopine dehydrogenase from mytilus galloprovincialis. | a cdna encoding the putative octopine dehydrogenase (ocdh) from the mussel mytilus galloprovincialis was cloned and sequenced. the complete coding region was expressed in the bacteria escherichia coli and the recombinant protein was purified. the m. galloprovincialis ocdh appears to have the highest affinity for the amino acid substrate l-arginine (88.22%), compared to l-alanine (9.04%) and glycine (2.74%). this enzyme showed no activity when taurine or ß-alanine was used as substrate. these dat ... | 2011 | 21791249 |
| investigation on the kinetic mechanism of octopine dehydrogenase. a regulatory behavior. | the kinetic scheme of octopine dehydrogenase of pecten maximus l., a monomeric enzyme obeying a bi-ter sequential mechanism, was completed, essentially in the forward reaction, by steady-state studies over a wide range of substrate concentration at ph 7.0. deviation from the michaelis-menten behavior with respect to nad+ and other significant kinetic data led us to ascribe for octopine dehydrogenase mechanism the mnemonical enzyme concept. in addition, another regulatory behavior can be envisage ... | 1994 | 205413 |
| sources of variation of escherichia coli concentrations in bivalve molluscs. | bivalve molluscs can concentrate contaminants, including pathogenic microorganisms, from the water column during their normal filter-feeding activity. in the european union, the risk of human and animal faecal contamination in bivalves is estimated by determining the concentration of escherichia coli in time-series samples from production areas. a structured field study was undertaken to determine the extent to which such concentrations varied between sites, sampling occasions and shellfish spec ... | 2013 | 23428551 |
| primary structure of myosin from the striated adductor muscle of the atlantic scallop, pecten maximus, and expression of the regulatory domain. | we have determined the complete cdna and deduced amino acid sequences of the heavy chain, regulatory light chain and essential light chain which constitute the molecular structure of myosin from the striated adductor muscle of the scallop, pecten maximus. the deduced amino acid sequences of p. maximus regulatory light chain, essential light chain and heavy chain comprise 156, 156 and 1940 amino acids, respectively. these myosin peptide sequences, obtained from the most common of the eastern atla ... | 2000 | 11129432 |
| biodiversity and dynamics of the bacterial community of packaged king scallop (pecten maximus) meat during cold storage. | the microbial biodiversity and dynamics of king scallops meat and coral during cold storage (cold chain rupture: 1/3 storage time at 4 °c followed by 2/3 at 8 °c), was assessed by combining culture-dependant and -independent methods. products were packaged as follows: aerobic, vacuum packed and 3 different co2/n2 modified atmospheres and the impact of these conditions on the microbial communities was assessed. results indicated that under air (current packaging condition), the dominant species c ... | 2013 | 23664260 |
| complete genome sequence of the phaeobacter gallaeciensis type strain cip 105210(t) (= dsm 26640(t) = bs107(t)). | phaeobacter gallaeciensis cip 105210(t) (= dsm 26640(t) = bs107(t)) is the type strain of the species phaeobacter gallaeciensis. the genus phaeobacter belongs to the marine roseobacter group (rhodobacteraceae, alphaproteobacteria). phaeobacter species are effective colonizers of marine surfaces, including frequent associations with eukaryotes. strain bs107(t) was isolated from a rearing of the scallop pecten maximus. here we describe the features of this organism, together with the complete geno ... | 2014 | 25197473 |
| deep transcriptome sequencing of pecten maximus hemocytes: a genomic resource for bivalve immunology. | pecten maximus, the king scallop, is a bivalve species with important commercial value for both fisheries and aquaculture, traditionally consumed in several european countries. major problems in larval rearing, however, still limit hatchery-based seed production. high mortalities during early larval stages, likely related to bacterial pathogens, represent the most relevant bottleneck. to address this issue, understanding host defense mechanisms against microbes is extremely important. in this st ... | 2014 | 24486903 |
| an in situ study of predator aggregations on scallop (pecten maximus (l.)) dredge discards using a static time-lapse camera system. | the impact of demersal fishing gears on benthic habitats and species has been the subject of much attention recently, and suggestions have been made that scavenging epifaunal species may benefit at the population level from the additional food source provided by discards. this paper investigates some aspects of this process, including the relative attractiveness to predators of different discard species, and the role of damage in scavenger attraction. a time-lapse video system with a 1000 m long ... | 2000 | 11090855 |
| liquid chromatography with mass spectrometry--detection of lipophilic shellfish toxins. | a rapid multiple toxin method based on liquid chromatography with mass spectrometry (lc/ms) was developed for the detection of okadaic acid (oa), dinophysistoxin-1 (dtx-1), dtx-2, yessotoxin (ytx), homoytx, 45-hydroxy-ytx, 45-hydroxyhomo-ytx, pectenotoxin-1 (ptx-1), ptx-2, azaspiracid-1 (aza-1), aza-2, and aza-3. toxins were extracted from shellfish using methanol-water (80%, v/v) and were analyzed using a c8 reversed-phase column with a 5 mm ammonium acetate-acetonitrile mobile phase under grad ... | 2016 | 16385985 |
| sequence analysis of the ribosomal dna internal transcribed spacer region in some scallop species (mollusca: bivalvia: pectinidae). | the internal transcribed spacer (its) region of the ribosomal dna from the european scallops aequipecten opercularis, mimachlamys varia, hinnites distortus, and pecten maximus was pcr amplified and sequenced. for each species, three or five clones were examined. the size ranged between 636 and 713 bp (its1, 209-276 bp; 5.8s rrna gene, 157 bp; its2, 270-294 bp) and gc content ranged between 47 and 50% (its1, 43-49%; 5.8s rrna gene, 56-57%; its2, 44-49%). variation within repeats was minimal; only ... | 2003 | 12897868 |
| insights from the shell proteome: biomineralization to adaptation. | bivalves have evolved a range of complex shell forming mechanisms that are reflected by their incredible diversity in shell mineralogy and microstructures. a suite of proteins exported to the shell matrix space plays a significant role in controlling these features, in addition to underpinning some of the physical properties of the shell itself. although, there is a general consensus that a minimum basic protein tool kit is required for shell construction, to date, this remains undefined. in thi ... | 2017 | 27744410 |
| characterization of the mantle transcriptome in bivalves: pecten maximus, mytilus edulis and crassostrea gigas. | the calcareous shells secreted by bivalve molluscs display diverse and species specific structural compositions, which indicates possible divergent biomineralization processes. thus, studying multiple mollusc species will provide a more comprehensive understanding of shell formation. here, the transcriptomes of the mantle tissues responsible for shell deposition were characterized in three commercially relevant bivalve species. using high-throughput sequencing and bioinformatics tools, de novo t ... | 2016 | 27160853 |
| mining the transcriptomes of four commercially important shellfish species for single nucleotide polymorphisms within biomineralization genes. | transcriptional profiling not only provides insights into patterns of gene expression, but also generates sequences that can be mined for molecular markers, which in turn can be used for population genetic studies. as part of a large-scale effort to better understand how commercially important european shellfish species may respond to ocean acidification, we therefore mined the transcriptomes of four species (the pacific oyster crassostrea gigas, the blue mussel mytilus edulis, the great scallop ... | 2016 | 26806806 |
| exploring the hologenome concept in marine bivalvia: haemolymph microbiota as a pertinent source of probiotics for aquaculture. | haemolymph-associated microbiota of marine bivalves was explored for antibacterial activity against important aquaculture pathogens. a collection of 843 strains were cultured from the haemolymph of four bivalve species (crassostrea gigas, mytilus edulis, pecten maximus and tapes rhomboides) collected by deep-sea diving in the glenan archipelago (france). cell-free culture supernatants were investigated for antibacterial activity using the well-diffusion assay. about 3% of haemolymph-associated c ... | 2014 | 24286558 |
| transformation of paralytic shellfish poisoning toxins in crassostrea gigas and pecten maximus reference materials. | matrix reference materials are an important requirement for the assessment of method performance characteristics and for routine quality control. in the field of marine toxin testing where biological assays have been used and where modern analytical testing methods are now becoming available, this requirement has become an urgent one. various approaches are utilised for preparation of such materials in the absence of available naturally occurring toxic shellfish samples. toxin-free shellfish may ... | 2012 | 22921579 |
| evidence of a tetradocosahexaenoic cardiolipin in some marine bivalves. | separation of phospholipid classes in lipid extracts from the scallop pecten maximus, the pacific oyster crassostrea gigas, and the blue mussel mytilus edulis was conducted using hplc. an isolated polar lipid fraction was found to contain a very high level of dha, up to 80 mol% of the total fa. ms with electrospray ionization in the positive-ion mode, tandem ms (ms-ms) and multidimensional nmr spectroscopy were used to analyze the detailed chemical structure of this polar lipid fraction. the iso ... | 2002 | 12056594 |
| characterization of a new alpha-l-fucosidase isolated from the marine mollusk pecten maximus that catalyzes the hydrolysis of alpha-l-fucose from algal fucoidan (ascophyllum nodosum). | algal fucoidan is an alpha-l-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. in an attempt to implement new enzymatic tools for structural study of this polysaccharide, we have found a fucosidase activity in the digestive glands of the common marine mollusk pecten maximus, which is active on a fucoidan extracted from the brown algae ascophyllum nodosum. we now report the purification and characterization of this ... | 2002 | 12042250 |
| sessile and mobile components of a benthic ecosystem display mixed trends within a temperate marine reserve. | despite recent efforts to increase the global coverage of marine protected areas (mpas), studies investigating the effectiveness of marine protected areas within temperate waters remain scarce. furthermore, out of the few studies published on mpas in temperate waters, the majority focus on specific ecological or fishery components rather than investigating the ecosystem as a whole. this study therefore investigated the dynamics of both benthic communities and fish populations within a recently e ... | 2015 | 25863362 |
| complete genome sequence of arcobacter sp. strain lft 1.7 isolated from great scallop (pecten maximus) larvae. | arcobacter sp. strain lft 1.7 was isolated from great scallop (pecten maximus) larvae. analysis of the 16s rrna gene sequence showed that strain lft 1.7 formed an independent lineage in the genus arcobacter the draft genome of lft 1.7 was sequenced to determine the taxonomic position and ecological function of this strain. | 2017 | 28183771 |
| draft genome sequences of neptuniibacter sp. strains lft 1.8 and atr 1.1. | we present the draft genomes of two strains previously identified as neptuniibacter sp. lft 1.8 (= cect 8936 = dsm 100781) and atr 1.1 (= cect 8938 = dsm 100783) isolated from larvae of great scallops (pecten maximus) and seawater, respectively. both strains surely constitute two novel species in this genus, with putative applications for aromatic compound degradation. | 2017 | 28153896 |
| arcobacter lekithochrous sp. nov., a new species isolated from a molluscan hatchery in norway. | four bacterial strains, lft 1.7t, lt2c 2.5, lt4c 2.8 and tm 4.6, were isolated from great scallop (pecten maximus) larvae and tank seawater in a norwegian hatchery and characterized by a polyphasic approach including phenotypic, chemotaxonomic and genomic traits. all of them were gram-negative and motile rods, oxidase and catalase positive and required sea salts for growth. major fatty acids present were summed feature 3 (c16:1ω7c/c16:1 ω6c), summed feature 8 (c18:1ω7c or c18:1 ω6c), c16:0, c14: ... | 2017 | 28109200 |
| neptuniibacter pectenicola sp. nov. and neptuniibacter marinus sp. nov., two novel species isolated from a great scallop (pecten maximus) hatchery in norway and emended description of the genus neptuniibacter. | nine isolates obtained from a great scallop hatchery in norway were characterized using a polyphasic approach. strains were gram-negative, aerobic and motile rods with oxidative metabolism. phylogenetic analysis based on the sequences of 16s rrna and rpob genes showed that these strains formed two different groups associated with members of the genus neptuniibacter. dna-dna hybridization (ddh) and average nucleotide identity (ani) demonstrated that the isolates constituted two novel species of t ... | 2017 | 28040300 |
| sinobacterium norvegicum sp. nov., isolated from great scallop (pecten maximus) broodstock and emended description of sinobacterium caligoides. | six isolates were recovered from great scallop (pecten maximus) broodstock in a hatchery in bergen, norway. the strains were thoroughly characterized by a polyphasic approach. phylogenetic analysis based on 16s rrna gene sequences indicated that these strains are related to the genus sinobacterium, showing sequence similarities between 96.97 and 97.63 % with the only species of the genus, sinobacterium caligoides. phenotypic characterization showed that the strains are typical marine halophiles, ... | 2015 | 26267335 |
| margolisiella islandica sp. nov. (apicomplexa: eimeridae) infecting iceland scallop chlamys islandica (müller, 1776) in icelandic waters. | wild iceland scallops chlamys islandica from an icelandic bay were examined for parasites. queen scallops aequipecten opercularis from the faroe islands and king scallops pecten maximus and queen scallops from scottish waters were also examined. observations revealed heavy infections of eimeriorine parasites in 95-100% of c. islandica but not the other scallop species. all life stages in the apicomplexan reproduction phases, i.e. merogony, gametogony and sporogony, were present. trophozoites and ... | 2011 | 21856309 |