| carbohydrases with unusual specificities--v. some properties of partially purified n-benzoyl-beta-d-glucosaminidase from the liver of the scallop mizuhopecten yessoensis. | | 1975 | 237730 |
| marine sterols. vii. synthesis of patinosterol, a minor constituent of sterols of the scallop, patinopecten yessoensis. | the structure of patinosterol, a minor sterol from the scallop, patinopecten yessoensis, was established as 22-trans-27-nor-(24s)-24-methyl-5alpha-cholest-22-en-3beta-ol by synthesis through wittig reaction. | 1977 | 910252 |
| marine sterols. vi(1). sterols of the scallop, patinopecten yessoensis. | the sterols of the scallop, patinopecten yessoensis jay, was found to contain over 20 components. the major components were delta5-sterols, and lesser amount of ring-saturated sterols were also present. biogenetically unusual c26 sterols (24-norcholesta-5,22-dien-3beta-ol and 24-norcholest-22-en-3beta-ol) and 24(28)-cis-24-propylidenecholest-5-en-3beta-ol (29-methylisofucosterol), 22-trans-27-nor-(24s)-24-methylcholesta-5,22-dien-3beta-ol (occelasterol), and a new sterol, 22-trans-27-nor-(24s)-2 ... | 1975 | 1209689 |
| [structure of the polysaccharide component of the bioglycan from the sea scallop patinopecten uessoensis]. | a bioglycan, [alpha]20d + 144 degrees (c 0.7, water), was isolated from the mantle of the scallop patinopecten yessoensis, which is widespread in the sea of japan. the bioglycan represents a complex of alpha-d-glucan (83%) and protein (13%) and was homogeneous as judged by gel-filtration and ultracentrifugation. by means of molecular-sieve chromatography on sepharose cl-4b, its average molecular weight was estimated as 4 mda. methylation studies, periodate oxidation and amylolysis showed that th ... | 1992 | 1301003 |
| calpain ii-like proteinase of scallop (patinopecten yessoensis) striated adductor muscle. | 1. a ca(2+)-dependent cysteine proteinase was purified from scallop striated adductor muscle by ammonium sulfate fractionation and column chromatography on deae-cellulose and sephacryl s-300. 2. the enzyme is of mr approximately 200,000, composed of two mr 100,000 subunits. 3. the enzyme is a cysteine proteinase with optimum activity at ph 6.8 and about 18 degrees c. in addition, it requires 1.7 mm ca2+ for half-maximal activity and more than 10 mm ca2+ for maximal activity. thus the enzyme can ... | 1992 | 1526121 |
| comparative studies on heat stability and autolysis of scallop (patinopecten yessoensis) calpain ii-like proteinase and rabbit calpain ii. | 1. the scallop calpain-like proteinase is about five times more labile than the rabbit calpain ii upon heat treatment at 35 degrees c. 2. by autolysis of the scallop proteinase of two 100 kda subunits, 90, 45 and 30 kda fragments were formed. thereby the activity decreased monophasically in the presence of millimolar order of ca2+, but did not increase in the presence of micromolar order of ca2+ unlike the rabbit calpain ii. | 1992 | 1526123 |
| effects of pectenophilus ornatus (copepoda) on the biomass of cultured japanese scallop patinopecten yessoensis. | there was a negative relationship between the number of parasitic copepods (pectenophilus ornatus) and the dry weight condition index of the infected japanese scallop (patinopecten yessoensis) cultured in the tsugaru strait of northern japan. the parasite is considered a serious pest of the commercial bivalve in cases of heavy infection. | 1992 | 1597810 |
| monoclonal antibodies toward scallop (patinopecten yessoensis) testis and wheat germ calmodulins. | a monoclonal antibody (im7) toward scallop testis calmodulin and another one (pbe2) toward wheat germ calmodulin were produced. ca2+ was required for im7 to react with scallop calmodulin. im7 reacted with the c-terminal region (asp78-lys148) of the calmodulin. as observed on competitive elisa, im7 reacted with chicken calmodulin, but not with euglena gracilis or wheat calmodulin, troponin c, myosin light chains, or parvalbumin. it is assumed that the cluster of thr143, thr146, and ser147 in the ... | 1991 | 1714441 |
| adenosine deaminase in the adductor muscle of the scallop, patinopecten yessoensis. | 1. the scallop enzyme was separated by de52 ion-exchange chromatography into two forms with the same mol. wt of 38,000 and similar characteristics. 2. the enzyme was inactivated in the absence of dithiothreitol and complete reactivation was achieved by adding the agent within a critical storage period. 3. the apparent values of pkm and vmax sensitively increased as ionic strength was raised to 250 mm and phosphate and sodium ions elevated the former value with a further increase of the ionic str ... | 1991 | 1959329 |
| the levels of prostaglandins associated with the reproductive cycle of the scallop, patinopecten yessoensis. | the present experiment was undertaken to investigate the seasonal variations of levels of prostaglandins (pgs) and regulation of these levels in the ovary and hemolymph of the scallop. the levels of prostaglandin f2 alpha (pgf2 alpha) and prostaglandin e2 (pge2) in the hemolymph and ovary increased during sexual maturation, and these levels in the ovary showed a marked increase in the spawning season. consecutive administration of antiestrogen inhibited the increase of the levels of pgf2 alpha a ... | 1990 | 2247614 |
| histopathological studies on experimental marine toxin poisoning--5. the effects in mice of yessotoxin isolated from patinopecten yessoensis and of a desulfated derivative. | the histopathological response of male icr mice to yessotoxin, isolated from the digestive organ of scallops patinopecten yessoensis, was compared with that of desulfated yessotoxin. the target organ of the former was the heart and those of the latter were the liver and pancreas. electron microscopically, marked intracytoplasmic edema in cardiac muscle cells was seen within 3 hr after the i.p. injection of over 300 micrograms/kg of yessotoxin. in contrast, desulfated yessotoxin at the same dose ... | 1990 | 2260107 |
| [location of immunoreactive leucine enkephalin in the digestive gland of the mollusca mizuhopecten yessoensis (jay)]. | distribution of leu-enkephalin-like peptides in the digestive gland of the japanese scallop has been investigated by means of the indirect immunohistochemical method. the immunoreactive leu-enkephalin is revealed in supranuclear vacuoles of separate large secretory cells. these cells are situated in epithelium of the digestive tubules along the gland periphery. their number is from 1-3 up to 5-6 per tubue section. the basal part of the cell and small apical vacuoles do not contain any products o ... | 1990 | 2350253 |
| calcium binding and calcium-sensitivity of heavy meromyosin and subfragment-1 from squid (todarodes pacificus) mantle and scallop (patinopecten yessoensis) adductor muscles. | 1. hmm and s-1 both bind one mol of calcium per mole of head, and a half of the calcium binding was diminished upon magnesium addition (10 mm) at the low affinity site. 2. the mg-atpase activity of hmm (without actin) was fully activated by the binding of one mol of calcium bound per mol of hmm. 3. the calcium binding profile to s-1 is the same as that to hmm, however, the mg-atpase activity of s-1 is independent of calcium binding. it is suggested that there are two kinds of myosin head (or s-1 ... | 1989 | 2523274 |
| cadmium accumulation in organs of the scallop mizuhopecten yessoensis--i. activities of phosphatases and composition and amount of lipids. | maintenance of mizuhopecten yessoensis in water with 0.5 mg cd/l for 60 days increased cd concentration in the mantle and ovaries about 200 times, in gills 70 times and hepatopancreas 20 times. alkaline phosphatase activity was inhibited in all organs except the hepatopancreas after a 1-month exposure to cd and became equal to the control in all the organs after 2 months exposure. molluscs transferred to flowing water showed a considerable decrease in the activity of alkaline and acid phosphatas ... | 1986 | 2872000 |
| cadmium accumulation in organs of the scallop mizuhopecten yessoensis--ii. subcellular distribution of metals and metal-binding proteins. | cd accumulation in the hepatopancreas and gills of the scallop resulted in an increase of metal concentration in the cytoplasm, while in control animals the general amount of cd in the hepatopancreas was concentrated in the microsomes and cytoplasm. cd appears to be equally distributed among high molecular weight proteins and metallothioneins in control animals. it was shown that during the experiment the metal was bound mainly to high molecular weight proteins. after 30 days of exposure of scal ... | 1986 | 2872001 |
| regulatory light chain-a myosin kinase (amk) catalyzes phosphorylation of smooth muscle myosin heavy chains of scallop, patinopecten yessoensis. | regulatory light chain-a myosin kinase (amk), which phosphorylates one of the myosin regulatory light chains, rlc-a, contained in the catch muscle of scallop, was also found to phosphorylate heavy chains of scallop myosin. after incubation of myosin isolated from the opaque portion of scallop smooth muscle (opaque myosin) with amk in the presence of [gamma-32p]atp, about 2 mol of 32p was incorporated per mol of the myosin. the radioactivity was mostly found in the heavy chain at 0.26 m kcl. the ... | 1988 | 2977385 |
| purification of a protein kinase phosphorylating myosin regulatory light chain-a (rlc-a) from smooth muscle of scallop, patinopecten yessoensis. | a protein kinase activity phosphorylating regulatory light chain-a (rlc-a) of scallop smooth muscle myosin was found to be present in scallop smooth muscle homogenate. the kinase was purified to homogeneity and named rlc-a myosin kinase (amk). amk was extracted from the muscle homogenate with a low salt solution and was purified by successive de-32 ion exchange chromatography, gel filtration on ultrogel aca 44, and affinity chromatography on sepharose 4b-6-aminohexyl-1-pyrophosphate. the molecul ... | 1986 | 3102465 |
| amino acid sequence of the essential light chain of adductor muscle myosin from ezo giant scallop, patinopecten yessoensis. | the amino acid sequence of the essential light chain (abbreviated as shlc) of adductor muscle myosin from ezo giant scallop (patinopecten yessoensis) was determined by conventional methods. the light chain was composed of 156 amino acid residues with proline and lysine as its amino and carboxyl termini, respectively. comparing this sequence with that of the shlc from bay scallop (aquipecten irradians), only 5 amino acid substitutions were recognized. the sequence homology between scallop and squ ... | 1987 | 3125162 |
| [localization of 17 beta-hydroxysteroid dehydrogenase in the gonads of bivalve mollusks--the sea pecten (patinopecten yessoensis jay) and gray's mussel (crenomytilus grayanus dunker)]. | presence of 17 beta-hydroxysteroid dehydrogenase (17 beta-hsd) in testes and ovaries of the common mussels--patinopecten yessoensis (jay) and crenomytilus grayanus (dunker) has been demonstrated histochemically. the enzyme is revealed in some granular amebocytes and germ cells. in growing oocytes its activity is higher that in oocytes completed their growth. 17 beta-hsd is revealed electron microscopically near agranular endoplasmic reticulum, or on the external surface of its membranes and in g ... | 1988 | 3242461 |
| [types of neurons in the central nervous system of the japanese scallop mizuhopecten yessoensis (jay)]. | composition of neurons, their structure and neuromediatory specialization in the japanese scallop ganglia have been studied by means of morphological, morphometrical and histochemical methods. three groups of neurons, differing in their histophysiological characteristics have been defined. large neurons are cholinergic ones. a well developed system of smooth membranes and large amount of cytosomes are specific for them. middle size and small neurons of the i type contain a granular endoplasmic r ... | 1988 | 3248046 |
| [action of cadmium on the elemental composition and cellular morphology of the hepatopancreas, kidneys and gonads of the mussel mytilus edulis and scallop mizuhopecten yessoensis]. | studies have been made on the content and distribution of cd, k, mg, ca, s and p in organs and cells of mussels and scallops before and after immersion in water containing cadmium chloride. in normal molluscs, the lowest content of cd was found in the gonads; cadmium content of the hepatopancreas and kidney in the scallop is correspondingly 20 and 40 times higher than in the mussel. after immersion in water with cadmium (250 micrograms/l in summer experiments and 500 micrograms/l in spring ones) ... | 1988 | 3414216 |
| in vitro effects of serotonin and prostaglandins on release of eggs from the ovary of the scallop, patinopecten yessoensis. | when ovarian pieces of the scallop, patinopecten yessoensis, were incubated in media containing serotonin (5-ht), the number of released eggs increased significantly. these eggs developed normally after fertilization. the threshold of the 5-ht effect reached a maximum at a concentration of 10(-6) m, and decreased sharply with increasing concentrations. methysergide, a 5-ht antagonist, completely inhibited the 5-ht-induced egg release. addition of aspirin or indomethacin to the incubation media a ... | 1987 | 3476349 |
| characterization of regulatory light chain-a myosin kinase from smooth muscle of scallop, patinopecten yessoensis. | the protein kinase that phosphorylates the regulatory light chain-a (rlc-a) of scallop smooth muscle myosin was isolated from scallop smooth muscle (sohma, h. & morita, f. (1986) j. biochem. 100, 1155-1163). the enzymatic properties of this kinase (amk) were investigated using rlc-a as the substrate. the km value for atp was 6.5 microm in the presence of 27 microm rlc-a at ph 7.0, and that for rlc-a was 133 microm in the presence of 1 mm atp. the vm value at saturation of both rlc-a and atp was ... | 1987 | 3584098 |
| phosphorylation of regulatory light chain a (rlc-a) in smooth muscle myosin of scallop, patinopecten yessoensis. | one of the two regulatory light chains, rlc-a, of scallop smooth muscle myosin was fully phosphorylated by myosin light chain kinase of chicken gizzard muscle. the residue phosphorylated was ser. it may be the ser at number 11 from the n-terminal. the sequence of 9 residues around the ser-11, qratsnvfa, is identical with that around the phosphorylatable ser of lc20 of chicken gizzard myosin. rlc-a was also phosphorylated slowly by camp-dependent protein kinase. the phosphorylation of rlc-a may b ... | 1985 | 3840802 |
| amino acid sequences of the two kinds of regulatory light chains of adductor smooth muscle myosin from patinopecten yessoensis. | smooth muscle myosin from scallop (patinopecten yessoensis) adductor muscle contains two kinds of regulatory light chains (regulatory light chains a and b), and myosin having regulatory light chain a is suggested to be suitable for inducing "catch contraction" rather than myosin having regulatory light chain b (kondo, s. & morita, f. (1981) j. biochem. 90, 673-681). the amino acid sequences of these two light chains were determined and compared. regulatory light chain a consists of 161 amino aci ... | 1985 | 4008467 |
| in vitro production of hydrogen peroxide by the amoebocytes of the scallop, patinopecten yessoensis (jay). | in vitro production of hydrogen peroxide (h2o2) by the amoebocytes of the scallop, patinopecten yessoensis, was studied. the authors first confirmed the availability of the direct quantitative method using homovanillic acid, and found that both resting and stimulated amoebocytes produced h2o2. however, the latter showed higher capacity for h2o2 production. the amoebocytes stimulated by concanavalin a released a large amount of h2o2 compared with the cells challenged with three species of bacteri ... | 1985 | 4043480 |
| [small granule-containing cells of the central nervous system of the bivalve mollusk patinopectin yessoensis (jay)]. | in the cns of the patinopecten yessoensis (jay) two types of cells have been revealed. the i type cells are typical unipolar neurons with a developed granular endoplasmic reticulum and golgi compex, with a nucleus containing small amount of chromatin. they possess elementary peptidergic granules. the ii type cells have in their cytoplasm and processes a large amount of electron-opague granules, specific for adrenergic systems. the nucleus is rich in clustered chromatin, the granular endoplasmic ... | 1985 | 4051758 |
| [parasitic ciliophora. i. pectenita golikowi gen. et sp. n. (entodiscidae fam. n.) from mizuhopecten yessoensis]. | | 1973 | 4204572 |
| [solitary type of oogenesis exemplified by the lamellibranch mollusc mizuhopecten yessoensis]. | | 1973 | 4574865 |
| [role of calcium ions in the generation of abdominal sense organ response in the scallop]. | the effect of a ca2+-free solution, cd2+ ions and theophylline on compound response recorded from the nerve which originates from the abdominal sensory organ of the scallop (patinopecten yessoensis) was studied during mechanical stimulation of this organ. it was shown that the elimination of ca2+ ions from the extracellular medium or addition of 10 mmol/l cd2+ caused complete reversible block of the response. the addition of 5 mmol/l theophylline to the medium resulted in a significant increase ... | 1984 | 6092977 |
| gonyautoxin associated with rna-containing fraction in the toxic scallop digestive gland. | a nontoxic high molecular substance associated with some paralytic shellfish poisons was separated by sephadex g-50 gel filtration from the toxic digestive glands of the scallop patinopecten yessoensis fed the causative plankton protogonyaulax tamarensis. unlike the corresponding fraction from the nontoxic digestive glands, the substance released gonyautoxins ii and iii on digestion with rnase t2, suggesting that it is associated with an rna of p. tamarensis. it is possible that the toxification ... | 1982 | 6181055 |
| amino acid sequences of the regulatory light chains of striated adductor muscle myosins from ezo giant scallop and akazara scallop. | amino acid sequences of the regulatory light chains of striated adductor muscle myosin from ezo giant scallop (patinopecten yessoensis) and akazara scallop (chlamys nipponensis akazara) were determined. tryptic peptides of each light chain were isolated and sequenced. the alignment of the tryptic peptides in each chain was deduced from the amino acid compositions and the partial sequences of peptic peptides of the ezo giant scallop light chain. the light chains both consist of 156 residues. hete ... | 1984 | 6706905 |
| [gonad innervation in bivalve mollusks]. | in marine lamellibranchia--patinopecten yessoensis (jay) and crenomytilus grayanus (dunker)--neural cells, small ganglia and neural fibres have been revealed in the gonad wall. the sensitive and effector parts are presented by rather variable morphological formations. by means of histochemical and ultrastructural investigations, functional affiliation of axons is revealed and their relations to tissue elements of the gonad are demonstrated. the effector neural apparatus is formed by peptidergic, ... | 1983 | 6838386 |
| [interaction of alpha-actinin and tropomyosin with f-actin]. | the interaction of alpha-actinin, f-actin and tropomyosin has been investigated by using electron microscopy, sds-gel electrophoresis and viscosimetry. it was shown that the temperature dependence of the interaction with f-actin was similar for alpha-actinins, isolated from the muscle of warmblooded animal (rabbit skeletal muscle) and from muscle of cold--blooded ones (cross striated part of the adductor of scallop patinopecten yessoensis). the temperature rise from 0 degrees c to 37 degrees c r ... | 1982 | 7073845 |
| smooth muscle of scallop adductor contains at least two kinds of myosin. | smooth muscle myosin purified from the small adductor of scallop, patinopecten yessoensis, showed four distinct bands in the region of low molecular weight upon urea-gel electrophoresis. the two components showing the lowest mobilities in the electrophoresis were assigned as regulatory light chains and named as rlc-a and rlc-b. the component showing the highest mobility was sh-light chain, and the other component was not identified. regulatory light chains of myosin prepared from the opaque port ... | 1981 | 7309694 |
| [alpha-actinin of primor'e scallop striated muscle. physico-chemical properties]. | a protein with chain weight of about 90,000 dalton (mp-90) was isolated from the cross-striated adductor muscle of the scallop patinopecten yessoensis. the physicochemical properties of mp-90 and its interaction with actin were studied in comparison with those of alpha-actinin of rabbit skeletal muscle. the data obtained allow us to conclude that this isolated protein is alpha-actinin of cross-striated adductor muscle of scallop patinopecten yessoensis. | 1980 | 7370318 |
| light removes inactivation of the a-type potassium channels in scallop hyperpolarizing photoreceptors. | the properties of light-activated and voltage dependent conductances in the hyperpolarizing photoreceptors of the isolated retina of the giant scallop (patinopecten yessoensis) have been studied in whole-cell voltage-clamp recordings. resting membrane potential of the cells was -35 to -60 mv in the dark. large outward current was maintained during illumination when the cell was voltage clamped at the resting potential. previous reports have shown that the hyperpolarizing response to light and th ... | 1995 | 7472411 |
| cadmium-induced alterations in essential trace element homeostasis in the tissues of scallop mizuhopecten yessoensis. | studies were performed regarding the effect of cadmium accumulation on the levels of essential elements (copper, zinc and iron) in the tissues of a marine bivalve mollusc, mizuhopecten yessoensis, exposed to cadmium at 250 ppb during 2 weeks. it was found that the concentration of cadmium in the tissues increased in the order gonads < gills < hepatopancreas < kidney during exposure time. however, the highest value of concentration factor was recorded in the gills. our data demonstrate that cadmi ... | 1995 | 7599982 |
| purification and characterization of beta-n-acetyl-d-hexosaminidase from the mid-gut gland of scallop (patinopecten yessoensis). | beta-n-acetyl-d-hexosaminidase was isolated from the mid-gut gland of patinopecten yessoensis. the enzyme was purified by making an acetone-dried preparation of the mid-gut gland, extracting with 50 mm citrate-phosphate buffer (ph 4.0) (about 13% of the extracted proteins was beta-n-acetyl-d-hexosaminidase), ammonium sulfate fractionation, and column chromatographies on cm-sepharose and deae-sepharose. the purified beta-n-acetyl-d-hexosaminidase was homogeneous on sds-page, and sufficiently free ... | 1993 | 7763885 |
| amino acid sequence of troponin c from scallop striated adductor muscle. | troponin and its ca(2+)-binding subunit troponin c (tnc) of the ezo-giant scallop, patinopecten yessoensis, have been revealed to bind only 1 mol of ca2+/mol irrespective of the presence and absence of mg2+. the amino acid sequence of the tnc has been determined by the automated edman degradation. tnc is composed of 152 residues including 3 tryptophans at positions 53, 70, and 109, 4 cysteines at positions 19, 31, 67, and 145, and no proline. the molecular weight is calculated as 17,410. the nh2 ... | 1994 | 8106387 |
| cloning and sequence of a cdna encoding akazara scallop troponin c. | a cdna clone encoding troponin c of akazara scallop (chlamys nipponensis akazara) striated adductor muscle was isolated and sequenced. the cdna is composed of 1987 bp and has an open reading frame of 462 bp at nucleotide positions 12-473. the amino acid sequence deduced from the cdna showed 89% homology with that of ezo-giant scallop (patinopecten yessoensis) troponin c (k. nishita, h. tanaka, and t. ojima, 1994, j. biol. chem. 269, 3464-3468). however, it showed lower homology with those of tro ... | 1994 | 8203890 |
| occurrence of tetrodotoxin in alexandrium tamarense, a causative dinoflagellate of paralytic shellfish poisoning. | a significant amount of tetrodotoxin was identified in the cultured cells of alexandrium tamarense, a causative dinoflagellate of paralytic shellfish poisoning. this result supports the hypothesis that both tetrodotoxin and paralytic shellfish toxins can be detected in organisms which possess either of them, although their proportion is different individually. the present result also supports our previous finding that a. tamarense is the origin of tetrodotoxin detected in the highly toxic scallo ... | 1996 | 8931250 |
| troponin from smooth adductor muscle of ezo-giant scallop. | troponin which can confer ca2+-sensitivity upon rabbit actomyosin mg-atpase activity has been prepared from the smooth adductor muscle of ezo-giant scallop (patinopecten yessoensis). the troponin comprises 40-, 20-, and 19-kda components. in order to characterize the components, they were separated from each other by cm-toyopearl column chromatography in the presence of 6 m urea. consequently, the 20-kda component was identified as troponin c, based on the ca2+-binding ability. the amount of ca2 ... | 1997 | 9133609 |
| a new method for the removal of toxic metal ions from acid-sensitive biomaterial | a new method (competitive adsorption method) for the removal of toxic heavy metals from acid-sensitive biomaterials was proposed and it was applied to the removal of cadmium from the mid-gut gland (mg) of scallop, patinopecten yessoensis. insolubilized humic acid, which has been developed in our laboratory, was used as a competitive adsorbent. a metal-complexation model was used to determine the adsorption characteristics of cadmium onto mg. furthermore, the model was applied to the competitive ... | 1997 | 9241157 |
| partial purification and characterization of six transglutaminases from ordinary muscles of various fishes and marine invertebrates. | six transglutaminases were prepared from ordinary muscles of scallop (patinopecten yessoensis), botan shrimp (pandalus nipponensis), squid (todarodes pacificus), carp (cyprinus carpio), rainbow trout (oncorhynchus mykiss), and atka mackerel (pleurogrammus azonus), and their physicochemical and enzymatic properties were compared with each other. the km value of carp transglutaminase for monodansyl cadaverine, a kind of primary amines, was 0.33 mm, while the other enzymes had km values of 0.01-0.0 ... | 1997 | 9440224 |
| phenotypic diversity of pseudoalteromonas citrea from different marine habitats and emendation of the description. | four strains of marine, aerobic, agar-decomposing bacteria with one polar flagellum and with dna g + c contents of 38.9-40.2 mol% were isolated from the far-eastern mussels crenomytilus grayanus and patinopecten yessoensis. these four strains were identified as pseudoalteromonas; however, they were phenotypically different from species described previously according to carbon compound utilization tests and the biolog identification system. high agar-decomposing activity was found in two strains, ... | 1998 | 9542094 |
| direct evidence of transformation of dinophysistoxin-1 to 7-o-acyl-dinophysistoxin-1 (dinophysistoxin-3) in the scallop patinopecten yessoensis. | non-toxic scallops patinopecten yessoensis were reared for 5 days with the toxic dinoflagellate dinophysis fortii sampled from the sea. the total cell number ingested by five scallops during the feeding experiment was approximately 682 x 10(3). okadaic acid (oa), dinophysistoxin-1 (dtx1) and esterified toxins of oa and dtx1 in d. fortii and scallop extracts were determined by liquid chromatography fluorescence detection (lc-fld) as their 9-anthryldiazomethane (adam) derivatives. only dtx1 was de ... | 1999 | 9920491 |
| effect of shellfish calcium on the apparent absorption of calcium and bone metabolism in ovariectomized rats. | fossil shellfish powder (fs) and ezo giant scallop shell powder (eg) were rendered soluble with lactate and citrate under decompression (fsex and egex, respectively) and we examined the effects of lactate-citrate solubilization of fs and eg on mineral absorption, tissue mineral contents, serum biochemical indices and bone mineral density (bmd) in ovariectomized (ovx) rats. the apparent absorption ratios of minerals tended to be high in the rats fed with the solubilized mineral sources, those in ... | 1999 | 10052120 |
| effects of removal and reconstitution of myosin regulatory light chain and troponin c on the ca(2+)-sensitive atpase activity of myofibrils from scallop striated muscle. | in order to examine the involvement of troponin-linked ca(2+)-regulation, in addition to well-known myosin-linked ca(2+)-regulation, in the contraction of molluscan striated muscle, myofibrils from ezo-giant scallop striated muscle were desensitized to ca(2+) by removing both myosin regulatory light chain and troponin c by treatment with a strong divalent cation chelator, cdta. the atpase level in the desensitized myofibrils was about half the maximum level in intact myofibrils regardless of the ... | 1999 | 10578052 |
| [actin depolymerizing action by marine toxin, pectenotoxin-2]. | pectenotoxin-2 (pctx-2), which is one of diarrhetic shellfish poisoning (dsp), is a family of cyclic polyether macrolide toxin isolated from scallop patinopecten yessoensis. although pctx-2 has a potent cytotoxic activities against several cancer cell lines, the biochemical activity of pctx-2 has not been determined yet. to clarify the biochemical activity of pctx-2 is the aime in this study. pctx-2 inhibited the contractions elicited by 72.7 mm kcl or 1 microm phenyrephrine in a concentration d ... | 1999 | 10629885 |
| the genomic structure of the scallop, patinopecten yessoensis, troponin c gene: a hypothesis for the evolution of troponin c. | two cdnas encoding troponin c (tnc) isoforms are isolated from the scallop, patinopecten yessoensis, striated adductor muscle. the sequential differences between these isoforms, named tnc(long) and tnc(short), are restricted in several residues of the c-terminal region. tnc(long) is commonly expressed in both the striated and the smooth adductor muscle; however, tnc(short) is only in the striated adductor muscle. the tnc gene is a single copy gene in the scallop, thus they are expressed through ... | 2000 | 10717478 |
| [effect of the hydra peptide morphogen on proliferation of spermatogonia in the scallop mizuhopecten yessoensis]. | | 2000 | 10752163 |
| molecular cloning of cdna encoding two subunits of calcineurin from scallop testis: demonstration of stage-specific expression during maturation of the testis. | complementary dnas encoding two subunits of scallop (patinopecten yessoensis) testis calcineurin were cloned, and the nucleotide sequences of their coding regions were determined. the deduced amino acid sequences of the catalytic subunit, calcineurin a (486 amino acid residues, m(r) 55,005.91), and the regulatory subunit, calcineurin b (170 residues, m(r) 19,237.67), showed high similarity to those of mammalian calcineurins, especially to the brain-type ones rather than to the testis-specific is ... | 2000 | 10788781 |
| absolute configuration at c45 in 45-hydroxyyessotoxin, a marine polyether toxin isolated from shellfish. | the marine polyether toxin, 45-hydroxyyessotoxin, was isolated together with yessotoxin from the scallop, patinopecten yessoensis. the 45-hydroxy group in the side chain was esterified with (s)- and (r)-alpha-methoxyalpha-trifluoromethylphenylacetic acids (mtpa). a detailed analysis of the 2d nmr spectra of the two esters established the r configuration at c45. | 2000 | 10993172 |
| isolation of a cdna encoding the motor domain of nonmuscle myosin which is specifically expressed in the mantle pallial cell layer of scallop (patinopecten yessoensis). | it has been reported that catch and striated muscle myosin heavy chains of scallop are generated through alternative splicing from a single gene [nyitray et al. (1994) proc. natl. acad. sci. usa 91, 12686-12690]. they suggested that the catch muscle type myosin was expressed in various tissues of scallop, including the gonad, heart, foot, and mantle. however, there have been no reports of the primary structure of myosin from tissues other than the adductor muscles. in this study, we isolated a c ... | 2000 | 11098141 |
| regulation of glycolysis during acclimation of scallops (patinopecten yessoensis jay) to anaerobiosis. | some glycolytic metabolites in the adductor muscle were measured after transfer of scallops from aerobic to anaerobic saltwater for 12 h. the level of octopine increased gradually during the initial 3 h incubation, and thereafter the level increased rapidly up to 12 h. the atp level also did not show any significant change for the initial 3 h, and then decreased rapidly. the fructose 2,6-biphosphate (fru 2,6-bp) level increased drastically during the initial 3 h incubation, but thereafter the le ... | 2000 | 11126751 |
| comparison of dinophysistoxin-1 and esterified dinophysistoxin-1 (dinophysistoxin-3) contents in the scallop patinopecten yessoensis and the mussel mytilus galloprovincialis. | okadaic acid (oa) homologues in toxic dinoflagellate dinophysis fortii, scallops patinopecten yessoensis and mussels mytilus galloprovincialis collected at the same site in mutsu bay, japan were determined by high-performance liquid chromatography fluorescence detection (hplc-fld) as their 9-anthryldiazomethane (adam) derivatives. prominent toxin in the scallops and the mussels was esterified dinophysistoxin-1 (dtx3) and dinophysistoxin-1 (dtx1), respectively, although only dtx1 was detected in ... | 2001 | 11137552 |
| comparison of volatile components in dried scallops (chlamys farreri and patinopecten yessoensis) prepared by boiling and steaming methods. | comparison of two types of dried scallops, chlamys farreri and patinopecten yessoensis, and effects of preparation methods (boiling and steaming) on the composition of their volatile components were carried out by simultaneous steam distillation and extraction and with analysis by gas chromatography-mass spectrometry. one hundred and seventy-two compounds were identified, three tentatively. qualitatively, both scallops had similar components. quantitatively, c. farreri contained more components ... | 2001 | 11170577 |
| fractal self-organization of the molluscan (mizuhopecten yessoensis) hemolymph cells capable of aggregation in vitro. | | 2000 | 11214467 |
| quantitative determination of marine toxins associated with diarrhetic shellfish poisoning by liquid chromatography coupled with mass spectrometry. | quantitative determination by liquid chromatography (lc) coupled with mass spectrometry (ms) was achieved for the following 10 toxins found in association with diarrhetic shellfish poisoning: okadaic acid (oa), dinophysistoxin-1 (dtx1), 7-o-palmitoylokadaic acid (paloa), 7-o-palmitoyldinophysistoxin-1 (pa1dtx1), pectenotoxin-1 (ptx1), pectenotoxin-2 (ptx2), pectenotoxin-2 seco acid (ptx2sa), pectenotoxin-6 (ptx6), yessotoxin (ytx), and 45-hydroxyyessotoxin (ytxoh). toxins in 2 g of the adductor ... | 2001 | 11217024 |
| identification and characterization of pectenotoxin (ptx) 4 and ptx7 as spiroketal stereoisomers of two previously reported pectenotoxins. | pectenotoxins (ptxs) isolated from the scallop patinopecten yessoensis were shown to be involved in an episode of diarrhetic shellfish poisoning (dsp). a total of eight analogues (ptx1-ptx7 and ptx10) have been isolated to date, and the structures of four of these analogues (ptx1, ptx2, ptx3, and ptx6) have already been elucidated. here, we report the characterization of ptx4 and ptx7 as 7-epi-ptx1 and 7-epi-ptx6, respectively, on the basis of nmr data and an acid-catalyzed chemical interconvers ... | 1998 | 11672107 |
| kinetic analysis of the bactericidal action of heated scallop-shell powder. | shell powder of scallop (patinopecten yessoensis) was exposed to heat treatment at between 200 and 1000 degrees c, and the bactericidal action of the powder slurry was investigated. shell powder heated at 700 degrees c or higher exhibited bactericidal action against escherichia coli, salmonella typhimurium, staphylococcus aureus and bacillus subtilis (vegetative cells). the death of bacteria in the shell powder slurry followed first-order reaction kinetics, and the apparent death rate constant ( ... | 2001 | 11789939 |
| pseudoalteromonas ruthenica sp. nov., isolated from marine invertebrates. | on the basis of phenotypic and genotypic characteristics and analysis of 165 rrna sequences, a novel species belonging to the genus pseudoalteromonas is described. two pale-orange-pigmented strains, kmm 300t and kmm 290, isolated respectively from a mussel, crenomytilus grayanus, and a scallop, patinopecten yessoensis, are marine, gram-negative, aerobic, rod-shaped bacteria that produce a number of antimicrobial compounds. the strains are able to degrade gelatin, elastin, starch, dna and tween 8 ... | 2002 | 11837308 |
| genetic manipulations in aquaculture: a review of stock improvement by classical and modern technologies. | the aim of this review was to highlight the extent to which the genetic technologies are implemented by the aquaculture industry. the review shows that some of the modern genetic technologies are already extensively applied by the diverse aquaculture industries, though not to the same extent for all important aquacultured species (according to fao 1998 figures). some species (common carp, atlantic salmon, rainbow trout, channel catfish, nile tilapia, and the pacific oyster) received concentrated ... | 2001 | 11841164 |
| tolerance to cadmium of free-living and associated with marine animals and eelgrass marine gamma-proteobacteria. | the tolerance to cd2+ and possible mechanisms of cd2+ detoxification by 178 free-living bacteria isolated from sea water, associated with marine animals (a mussel crenomytilus grayanus, a scallop patinopecten yessoensis), and eelgrass zostera marina collected in the sea of japan and the sea of okhotsk have been studied. the concentrations of 25 and 50 mg cd2+/l were highly toxic and inhibited the growth from 54% to 78% of the total bacteria studied. the free-living bacteria isolated from seawate ... | 2002 | 11927987 |
| proteolytic substructure of myorod, a thick filament protein of molluscan smooth muscles. | myorod (mr), a new thick filament protein of molluscan smooth muscles, is an alternatively spliced product of the myosin (mn) heavy chain gene. we studied digestion of mr and mn from the posterior adductor of crenomytilus grayanus and the outer portion of adductor of mizuchopecten (patinopecten) yessoensis by papain and constructed the proteolytic substructure of mr, that is an analogue to mn substructure. there are a head domain (analogue of mn s1) and a rod domain (analogue of mn rod); the jun ... | 2002 | 12223213 |
| [a study on the contents of fat and fatty acids in five shellfishes]. | the contents of fat and fatty acids in callista erycina (linnaeus), paphia (paratapes) undulata (born), meretrix meretrix (linnaeus), chlamys farreri (jones et preston) and patinopecten yessoensis (jay) were studied. fat was extracted with bligh & dyer (b&d) method. the lipid classes were transesterified with potassium hydroxide in methanol. fatty acid methyl esters (fames) were assayed with gc-ms and polar capillary column (hp-innowax 30 m x 0.25 mm i.d. x 0.25 micron). gc injector temperature ... | 2001 | 12541656 |
| vitellogenin synthesis in the ovary of scallop, patinopecten yessoensis: control by estradiol-17 beta and the central nervous system. | elucidation of a profile of scallop vitellin formation associated with oogenesis and its endocrine control, and identification of a vitellogenin synthesizing site were immunologically undertaken by using anti-scallop vn serum. vn content increased during ovarian growth and accounted for more than 80% of the water soluble protein of the ovary at the mature stage. in vivo injection of estradiol-17 beta (e(2)) resulted in an increase in vn content in the ovary. in vitro accumulation of vn in the ov ... | 2003 | 12975805 |
| purification and properties of an aminopeptidase from the mid-gut gland of scallop (patinopecten yessoensis). | an aminopeptidase was isolated from the mid-gut gland of patinopecten yessoensis. the enzyme was purified from an acetone-dried preparation by extracting, ammonium sulfate precipitation, hi-load q column chromatography, isoelectric focusing, and poros hp2 and hq column chromatography. the molecular weight of the enzyme was estimated to be 61 kda by sds-polyacrylamide gel electrophoresis and 59 kda by gel permeation chromatography. the isoelectric point of the enzyme was 5.2 and the optimum ph wa ... | 2003 | 14662315 |
| molecular cloning and expression analysis of vitellogenin in scallop, patinopecten yessoensis (bivalvia, mollusca). | the present study was undertaken to determine scallop vitellogenin (vtg) cdna sequence, to identify vtg synthesizing cell, and to analyze the regulation of vtg mrna expression. clones containing partial cdna sequence of vtg were isolated from cdna library of the scallop ovary by immunoscreening with the anti-scallop vitellin (vn) serum. the deduced amino acid sequence of the clone containing the longest cdna insert (1,689 bp) was identified as a member of the lipid transport protein family and e ... | 2004 | 14735488 |
| the complete primary structure of molluscan shell protein 1 (msp-1), an acidic glycoprotein in the shell matrix of the scallop patinopecten yessoensis. | the complete primary structure of msp-1, a major water-soluble glycoprotein in the foliated calcite shell layer of the scallop patinopecten yessoensis, is reported. the full-length complementary dna for msp-1 isolated by polymerase chain reaction contained a sequence for a signal peptide of 20 amino acids followed by a polypeptide of 820 amino acids with calculated molecular mass of 74.5 kda. the deduced amino acid sequence of msp-1 includes a high proportion of ser (32%), gly (25%), and asp (20 ... | 2001 | 14961352 |
| molecular characterization of a mitochondrial dna segment from the japanese scallop (patinopecten yessoensis): demonstration of a region showing sequence polymorphism in the population. | a 1.3-kb mitochondrial dna segment from the japanese scallop patinopecten yessoensis was cloned and sequenced. this segment contained the transfer rna(met) gene and partial sequences of 2 ribosomal rna genes, together with 2 separate noncoding regions (designated ncr1 and ncr2). the ncr regions derived from 78 individuals cultured in lake saroma or matsu bay, were sequenced, and we found 15 loci with sequence alterations including 13 substitutions, 1 deletion, and 1 insertion (1 locus in ncr1, 1 ... | 2001 | 14961353 |
| substrate specificity of aminopeptidase from the mid-gut gland of the scallop (patinopecten yessoensis). | an action for various peptides and a kinetic study for amino acid p-nitroanilides (pnas) and 4-methylcoumaryl-7-amides (mcas) were performed with purified aminopeptidase from the mid-gut of the scallop. the enzyme preferred dipeptides having ala, met, and phe in the amino-terminal or the penultimate position from the amino-termini. the catalytic efficiencies, k(cat)/k(m) values for ala-pna and mca were the highest in the tested substrates, and those for pna and mca substrates having met or phe w ... | 2004 | 15118330 |
| estrogen synthesis in relation to gonadal development of japanese scallop, patinopecten yessoensis: gonadal profile and immunolocalization of p450 aromatase and estrogen. | aromatase activities and estrogen contents in the gonad of japanese scallop, patinopecten yessoensis, were determined during gonadal development and estrogenic cells in the testis were identified immunohistochemically. ovaries and testes developed rapidly during january and february to reach the mature stage in march and the spawning stage in april. increases in aromatase activities of the ovary and testis preceded the onset of the ovarian and testicular development. aromatase activities reached ... | 2004 | 15364295 |
| granulin-like peptide in the mid-gut gland of the bivalve mollusk, patinopecten yessoensis. | a cysteine-rich polypeptide, termed crp1, with a molecular mass of 5829 da was found to occur in the mid-gut gland of the scallop patinopecten yessoensis. crp1 was purified by reverse phase and cation-exchange chromatographies. the amino acid sequence of crp1 was deduced from its n-terminal amino acid sequence, amino acid composition and the sequence of a partial cdna, indicating that crp1 is a 57-amino-acid polypeptide containing 12 cysteine residues with a calculated molecular mass of 5841 da ... | 2004 | 15535978 |
| complementary dna cloning and molecular evolution of opine dehydrogenases in some marine invertebrates. | the complete complementary dna sequences of genes presumably coding for opine dehydrogenases from arabella iricolor (sandworm), haliotis discus hannai (abalone), and patinopecten yessoensis (scallop) were determined, and partial cdna sequences were derived for meretrix lusoria (japanese hard clam) and spisula sachalinensis (sakhalin surf clam). the primers odh-9f and odh-11r proved useful for amplifying the sequences for opine dehydrogenases from the 4 mollusk species investigated in this study. ... | 2004 | 15565272 |
| genetic structure of japanese scallop population in hokkaido, analyzed by mitochondrial haplotype distribution. | to examine the genetic structure of japanese scallop populations (mizuhopecten yessoensis) in hokkaido prefecture, japan, and compare it with those in the aomori prefecture, we applied a method for lineage analysis based on sequence variation in a mitochondrial dna segment (ncr2). after showing that there was a low probability of doubly uniparental inheritance of mitochondrial dna in the scallop, we sequenced the ncr2 regions of 914 individuals from 15 populations (13 in hokkaido and 2 in aomori ... | 2005 | 15806334 |
| development of microsatellite markers for japanese scallop (mizuhopecten yessoensis) and their application to a population genetic study. | the japanese scallop (mizuhopecten yessoensis) is one of the main fishery products in japan, but with the expansion of culture operations of the japanese scallop, various problems have been encountered including high mortality, poor growth, poor seed production, and so on. moreover, there is concern that many years of cultivation may have affected the genetic structure of the scallop population. to approach these problems and concerns, we developed microsatellite markers as a molecular tool for ... | 2005 | 16206016 |
| detection of renibacterium salmoninarum, the causative agent of bacterial kidney disease in salmonid fish, from pen-cultured coho salmon. | the detection of renibacterium salmoninarum antigen from pen-cultured coho salmon was attempted. flounder (limanda herzensteini) (n = 24), greenling (hexagrammos otakii) (n = 5), japanese sculpin (cottus japonicus) (n = 1), and flathead (platycephalus indicus) (n = 22) captured by fishing around coho salmon net pens were examined for the presence of r. salmoninarum antigen by an indirect dot blot assay and by an indirect fluorescent-antibody technique using polyclonal and monoclonal antibodies. ... | 1992 | 16348666 |
| relationship between shell weight and cadmium content in whole digestive gland of the japanese scallop patinopecten yessoensis (jay). | seasonal and age-specific variations of cadmium (cd) concentration in the digestive gland were investigated in the japanese scallop patinopecten yessoensis from peter the great bay, sea of japan, with different degrees of cd pollution. the seasonal changes in cd concentrations of the digestive gland were inversely proportional to the dry weight of the gland. concentrations of cd and total cd content (mug cd per organ) increased with age (age-specific) to the same extent in contaminated and uncon ... | 2006 | 16426675 |
| purification, cdna cloning and homology modeling of endo-1,3-beta-d-glucanase from scallop mizuhopecten yessoensis. | the retaining endo-1,3-beta-d-glucanase (lv) with molecular mass of 36 kda was purified to homogeneity from the crystalline styles of scallop mizuhopecten yessoensis. the purified enzyme catalyzed hydrolysis of laminaran as endo-enzyme forming glucose, laminaribiose and higher oligosaccharides as products (km approximately 600 microg/ml). the 1,3-beta-d-glucanase effectively catalyzed transglycosylation reaction that is typical of endo-enzymes too. optima of ph and temperature were at 4.5 and 45 ... | 2006 | 16473536 |
| a novel oocyte maturation arresting factor in the central nervous system of scallops inhibits serotonin-induced oocyte maturation and spawning of bivalve mollusks. | serotonin (5-hydroxytriptamine; 5-ht) is a major neurotransmitter that triggers oocyte maturation and sequential spawning in bivalve mollusks. a proteinous and heat-labile substance that proved to be a novel inhibitor of 5-ht-induced egg release from ovarian tissue was found in the cerebral and pedal ganglia (cpg) of the scallop patinopecten yessoensis. the same inhibitory activity was also observed in the proteinous fraction from the supernatant of hemolymph. histological observation demonstrat ... | 2006 | 16574113 |
| [lipophilic toxin profiles associated with diarrhetic shellfish poisoning in scallops, patinopecten yessoensis, collected in hokkaido and comparison of the quantitative results between lc/ms and mouse bioassay]. | lipophilic toxins associated with diarrhetic shellfish poisoning (dsp) in scallops, patinopecten yessoensis, collected in hokkaido, japan were quantified by liquid chromatography-mass spectrometry (lc/ms). pectenotoxin-6 (ptx6) and yessotoxin (ytx) were the dominant toxins in the scallops, although the percentages of these toxins were different depending on the production area or the sampling period. the quantitative results obtained for the scallops in lc/ms and in mouse bioassay (mba) were com ... | 2006 | 16729662 |
| chromosome rearrangements in pectinidae (bivalvia: pteriomorphia) implied based on chromosomal localization of histone h3 gene in four scallops. | chromosomal structural rearrangement in four scallops, chlamys farreri (n=19), patinopecten yessoensis (n=19), chlamys nobilis (n=16) and argopecten irradians (n=16), was studied by fluorescence in situ hybridization using histone h3 gene probes. the results show that histone h3 gene sites differ strikingly with regard to number, location, and intensity among, or even within these species. for example, two histone h3 gene loci were detected on the metaphase chromosomes of p. yessoensis, while on ... | 2007 | 16909332 |
| [feeding and growth of japanese scallop inhabiting different bottom sediment types]. | two parts of the population of japanese scallop mizuhopecten yessoensis inhabiting sites with different bottom sediments in an open part of peter the great bay of the sea of japan were compared. the scallops grew slower on muddy site compared to sandy site despite better food availability at muddy site. the food sources were determined using fatty acids as biomarkers. analysis of the fatty acid composition of the scallops has identified diatom plankton, flagellates, and invertebrate larvae as th ... | 2007 | 17352202 |
| two novel elements (cfg1 and pyg1) of mag lineage of ty3/gypsy retrotransposons from zhikong scallop (chlamys farreri) and japanese scallop (patinopecten yessoensis). | two novel elements (cfg1 and pyg1) of mag lineage of ty3/gypsy retrotransposons were cloned from zhikong scallop (chlamys farreri) and japanese scallop (patinopecten yessoensis). the total length of the cfg1 element is 4826 bp, including 5'-ltr (192 bp), the entire orf (4047 bp) and 3'-ltr (189 bp). the entire orfs of both cfg1 and pyg1 elements are composed of 1348 aa and do not have any frameshifts. their closest relative is jule element from the poeciliid fish (xiphophorus maculatus). on aver ... | 2008 | 17694394 |
| heat treatment of scallop adductor muscle using superheated steam. | scallop (patinopecten yessoensis) adductor muscles were heated using superheated steam (150 and 200 degrees c), boiling (98 degrees c), and normal steaming (95 degrees c). the amounts of amino acids, water-soluble peptides, and nucleotides, expressed as extractive nitrogen in scallop products, are very important elements of quality and taste. after 15-min heating of scallop muscles with normal steaming and boiling, respective losses of 50% and 64% of the extractive nitrogen were observed. howeve ... | 2007 | 17995678 |
| mycosporine-like amino acids extracted from scallop (patinopecten yessoensis) ovaries: uv protection and growth stimulation activities on human cells. | scallops (patinopecten yessoensis) are extensively cultured and landed in japan. during the processing of scallops, large amounts of internal organs and shells are discharged as industrial wastes. to reduce the burden on the environment, effective utilization and disposal methods of the wastes are required. therefore, we have screened for useful materials in scallop internal organs, and found ultraviolet (uv) absorbing compounds from scallop ovaries. based on uv absorption, electrospray ionizati ... | 2008 | 18157682 |
| studies of diarrhetic activity on pectenotoxin-6 in the mouse and rat. | diarrhetic activity of pectenotoxin-6 (ptx6), a shellfish contaminant in japanese scallops (patinopecten yessoensis), was studied in vivo. mice gavaged with 5mg/kg ptx6 did not show diarrhea or fluid secretion, and no prominent pathological changes were observed. there was no synergistic toxicity of ptx6 with okadaic acid (oa) or pectenotoxin-2 (ptx2) when toxins were given to mice by gavage. synergistic activity of ptx6 with oa was also not confirmed under crude conditional simulation with oil. ... | 2008 | 18241906 |
| application of sub-critical water technology for recovery of heavy metal ions from the wastes of japanese scallop patinopecten yessoensis. | sub-critical water (sub-cw) technology was used as a new technology with environmental and financial benefits for the recovery of harmful heavy metal ions cd (ii), zn (ii), cu (ii), fe (ii), mn (ii) and ni (ii) in the waste of japanese scallop patinopecten yessoensis. the metals are responsible for environmental problems owing to the large amount of the waste. this study proposes a new method using sub-cw treatment to recover the metal ions from scallop waste and simultaneously produce harmless ... | 2008 | 18452973 |
| pancreatic lipase-related protein (py-plrp) highly expressed in the vitellogenic ovary of the scallop, patinopecten yessoensis. | a cdna (1206 bp) encoding a pancreatic lipase-related protein (py-plrp) was obtained from the ovary of the scallop, patinopecten yessoensis, using a differentially expressed gene system. the open reading frame specified a protein containing 353 amino acids (~38 kda). the n-terminal catalytic domain, which contained the catalytic triad of serine, aspartate, and histidine residues, 10 cysteine residues involved in disulfide bridges, and the conserved lid domain, indicated that the protein would be ... | 2008 | 18602322 |
| the manganese superoxide dismutase gene in bay scallop argopecten irradians: cloning, 3d modelling and mrna expression. | a novel manganese superoxide dismutase (mnsod) was cloned from bay scallop argopecten irradians by 3' and 5' rapid amplification of cdna ends (race) pcr. the full-length cdna of mnsod was of 1207 bp with a 678 bp open reading frame encoding 226 amino acids. the deduced amino acid sequence contained a putative signal peptide of 26 amino acids. sequence comparison showed that the mnsod of a. irradians shared high identity with mnsod in invertebrates and vertebrates, such as mnsod from abalone hali ... | 2008 | 18674624 |
| yessotoxins, a group of marine polyether toxins: an overview. | yessotoxin (ytx) is a marine polyether toxin that was first isolated in 1986 from the scallop patinopecten yessoensis. subsequently, it was reported that ytx is produced by the dinoflagellates protoceratium reticulatum, lingulodinium polyedrum and gonyaulax spinifera. ytxs have been associated with diarrhetic shellfish poisoning (dsp) because they are often simultaneously extracted with dsp toxins, and give positive results when tested in the conventional mouse bioassay for dsp toxins. however, ... | 2008 | 18728761 |
| [thymidine and thymidylate kinases from the scallop mizuhopecten yessoensis gonads]. | thymidine and thymidylate kinases were isolated from the gonads of scallop mizuhopecten yessoensis. the enzymes were purified 537- and 100-fold, respectively, and were free of phosphatase and atpase impurities. ions of bivalent metals and atp were necessary for both the nucleoside and nucleotide kinase activities; the ph optimum fall into the range of 7.5-8.5. kcl and nacl at a concentration of up to 100 mm had no inhibiting effect on the activities of these scallop enzymes. thymidine kinase cat ... | 2008 | 18822769 |
| [age-related variation of adductor weight in the japanese scallop mizuhopecten yessoensis (jay) depending on cultivation technique]. | age-related variation in the absolute and relative adductor weight has been studied for 8 years in six samples of japanese scallops mizuhopecten yessoensis (jay) of different generations from a shellfish farm in alekseev bight, sea of japan. the test character has proved to vary widely within the samples, with variation in shell size being relatively low. it has been shown that the dependence of adductor weight on the age of the scallop is not absolute: even-aged samples from different generatio ... | 2008 | 18956743 |
| comparative mitogenomic analyses of three scallops (bivalvia: pectinidae) reveal high level variation of genomic organization and a diversity of transfer rna gene sets. | it can be seen from the available mollusk mitogenomes that the family pectinidae exhibits the most variation in genome organization. in this study, comparative mitogenomic analyses were performed for three scallops from the subfamily chlamydinae (pectinidae), with the goal of characterizing the degree of variability of mitogenome organization and other characteristics among species from the same subfamily and exploring their possible evolution route. | 2009 | 19416513 |
| [the construction of a preliminary genetic linkage map in the japanese scallop mizuhopecten yessoensis]. | aflp markers were used to construct the primary linkage map in a family of mizuhopecten yessoensis. a total of 1 855 markers were generated in two parents and 52 progenies of the mapping family by using 56 aflp primer combinations. among the 1 855 markers, 598 were polymorphic and 354 were in agreement with the mendelian segregating ratio of 1:1. markers segregated according to mendelian 1:1 ratio (p>0.05) and 23 distorted markers (0.01<p<0.05) were used in linkage analysis. the male map was com ... | 2009 | 19586864 |
| molecular cloning, characterization, and the response of manganese superoxide dismutase from the antarctic bivalve laternula elliptica to pcb exposure. | manganese superoxide dismutase (lemnsod) cdna was cloned from the antarctic bivalve laternula elliptica. the full-length cdna of lemnsod is 1238 bp in length and contains an open reading frame of 681 bp encoding 226 amino acid residues including a putative mitochondrial targeting peptide of 26 amino acids in the n-terminal region. the calculated molecular mass is 24.8 kda with an estimated isoelectric point of 6.75. lemnsod signatures from 185 to 192 (dvwehayy) and four conserved amino acids (h5 ... | 2009 | 19628043 |
| the mitochondrial genomes of two scallops, argopecten irradians and chlamys farreri (mollusca: bivalvia): the most highly rearranged gene order in the family pectinidae. | molluscs in general and bivalves in particular, exhibit an extraordinary degree of mitochondrial gene order variation when compared with other metazoans. here, we determined the mitochondrial genomes of two scallops argopecten irradians and chlamys farreri. the complete mitochondrial genome of a. irradians is 16,211 nts in length and the nearly complete mitochondrial genome of c. farreri is 20,789 nts in length. both of the genomes contain 35 genes including 12 protein-coding genes, 2 ribosomal ... | 2010 | 20013337 |
| impact of the pectenotoxin c-43 oxidation degree on its cytotoxic effect on rat hepatocytes. | the metabolism of toxins that have accumulated in fish and shellfish is considered a detoxification process, as happens with pectenotoxins (ptxs) in the japanese scallop patinopecten yessoensis. ptxs are macrolactones that display hepatotoxicity in mice, principally by capping or sequestering actin, their molecular target. ptx-2, which is considered to be the parental compound, oxidizes progressively to ptx-1, ptx-3, and ptx-6 in the japanese scallop. in this study, we observed that ptx-1, ptx-6 ... | 2010 | 20085294 |