| the disulfide bridges of the trypsin-kallikrein inhibitor k from snails (helix pomatia). thermal inactivation and proteolysis by thermolysin (author's transl). | isoinhibitor k is the main component of the complex mixture of isoinhibitors of broad specificity secreted into the mucus by the roman snail (helix pomatia). the disulfide pairing was determined after the amino acid sequence had been elucidated. two cystine-containing peptides with the disulfide bridges cys32-cys53 and cys32-cys53 plus cys7-cys57 were obtained after thermolytic hydrolysis of the native inhibitor at 80 degrees c and chromatographic separation of the peptides using se-sephadex. th ... | 1976 | 3462 |
| tyrosine hydroxylase in snail (helix pomatia) nervous tissue. | | 1976 | 5096 |
| [immunologic properties of various cell fractions of a wild strain and a mutant strain of saccharomyces cerevisiae hansen]. | some fractions of the saccharomyces cerevisiae cell wall have been prepared by the action of helix pomatia juice on intact cells. immunosera were obtained by injecting rabbits with these fractions. immunofluorescence reactions, obtained with these sera, show that some fractions of mannopeptides when extracted from a "smooth-colony" mutant strain, have lost antigenic determinants. | 1977 | 70263 |
| [electron microscopic detection of blood group antigen a on human erythrocytes by means of ferritin- and gold-labelled protectin of helix pomatia]. | by means of ferritin- and gold-labelled protectin from the albumen gland of the edible snail helix pomatia the blood group antigen a was located on human erythrocytes of groups a1, a2 and a1b. with erythrocytes of groups o and b the reaction is negative. the antigen is focally distributed on the outer surface of the cell membrane. cells from groups a1 and a1b have an antigen a concentration about 4 times greater than a2 cells. the numbers of particles after tagging with ferritin or gold are comp ... | 1976 | 134531 |
| universality of gonadotropic hormone effect. effect of mammalian fsh on the ovotestis of the snail helix pomatia. | gonadotropic hormone (fsh) stimulated markedly spermatogenesis in the ovotestis of snails. this is indicative of the presence of receptors sensitive to gonadotropic hormone in snail spermatogonia. fsh had no influence on oogenesis. | 1975 | 168707 |
| hydrolysis of steroid glucuronides with beta-glucuronidase preparations from bovine liver, helix pomatia, and e. coli. | we determined the enzymic activity of beta-glucuronidase preparations from bovine liver, helix pomatia, and escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. we also studied the effect of na2so4 on the enzymic hydrolysis of several substrates with the three preparations of beta-glucuronidase. na2so4 increases the rate of hydrolysis of all substrates with beta-glucuronidase from bovine liver. hydrolysis of a steroid glucoronide with beta-glucuronidase from hel ... | 1977 | 189953 |
| adherent cells as targets in mitogenic t-lymphocyte stimulation: replacement of lymphocyte-bound mitogens by nonmitogenic substances. | the sequence of events in mitogenic t-lymphocyte activation was investigated by employing purified mouse peritoneal adherent cells and t-lymphocyte populations. after treatment of adherent cells with the oxidizing agents sodium periodate or neuroaminidase plus galactose oxidase and removal of the mitogens, the cells acquired the ability to stimulate purified t-lymphocytes added subsequently. for this process of t-lymphocyte activation, the additional presence of the membrane interacting substanc ... | 1979 | 226472 |
| neuraminidase inhibition by chemically sulphated glycopeptides. | chemically sulphated glycopeptides (derived from pig duodenal mucosa) inhibited clostridium perfringens neuraminidase (ec 3.2.1.18) activity in a ph-dependent manner. analysis of inhibition kinetics data indicated that, although the enzyme inhibition could not be categorized into any of the classical types of inhibition, it could be interpreted as a function of the size and shape of the substrates used. the enzyme activity was inhibited by 86% and 40% when tested with bovine submaxillary-gland m ... | 1979 | 227363 |
| quantitative precipitin studies on the specificity of an extract from tridacna maxima (röding). | haemolymph from the clam tridacna maxima precipitated with purified h-blood-group substances, helix pomatia galactogen, and pneumococcus type xiv polysaccharide. although gel diffusion, gel electrophoresis, and inhibition experiments indicated that only a single precipitating lectin was present in the haemolymph, quantitative precipitin and haemagglutination results suggested that a second agglutinin with anti-h-like specificity was also present. evidence obtained from hapten inhibition experime ... | 1975 | 236090 |
| [gelelectrofocusing of protektin anti ahp from the albumin gland of helix pomatia (author's transl)]. | the protektin anti ahp from the albumine gland of helix pomatia is a very interesting substance because of its ability to agglutinate erythrocytes of blood group a as well as group c streptococci. purification is possible by reversible absorption on sephadex g200. isoelectric focusing in a sucrose density gradient is difficult, because the protein precipitates at the isoelectric point. the agglutinin, which is homogeneous in disk electrophoresis experiments (in an alkaline gel system as well as ... | 1975 | 242131 |
| enzyme immunoassay for total oestrogens in pregnancy plasma or serum. | we have developed an enzyme immunoassay for total oestrogens in pregnancy serum of plasma, using horseradish peroxidase (hrp) as the marker enzyme. a test combination consisting of an antiserum against oestriol-16/17-monosuccinyl-albumin and oestriol-16/17-monosuccinyl-hrp yielded a sensitive system, which reacted to approximately the same extent with oestrone, oestradiol, oestriol and their 16- and 17-conjugates. samples had to be diluted 1 to 10 to avoid interference of plasma factors with the ... | 1978 | 361293 |
| characterization of human lymphocytes bearing fc receptors for ige isolated from blood and lymphoid organs. | human lymphocytes isolated from adult peripheral blood and cord blood, tonsils, adenoids and spleens were analysed for fc receptors for ige (fcepsilon) by rosette assays. the fcepsilon+ cells were also characterized for their class of surface immunoglobulin (sig), complement receptors, receptors for sheep erythrocytes (e), and helix pomatia a haemagglutinin (hp), and their abilities to phagocytoze and adhere. the average number of fcepsilon+ cells was in adult peripheral blood 1.2 +/- 0.4%, in c ... | 1979 | 368962 |
| mutagenicity of plant flavonols in the salmonella/mammalian microsome test: activation of flavonol glycosides by mixed glycosidases from rat cecal bacteria and other sources. | over 70 naturally occurring and synthetic flavonoids were screened for mutagenicity with 5 tester strains in the salmonella/mammalian microsome assay: ta1535, ta100, ta1537, ta1538 and ta98. frameshift mutagenicity was confined to the flavonols (flavon-3-ols) in strain ta98, ta1537 and ta100. the two most mutagenic falvonols, namely, quercetin (3,3',4',5,7-pentahydroxyflavone) and kaempferol (3,4',5,7-tetrahydroxyflavone), exhibiting 12 and 7 revertants/nmol in ta98 respectively, are also the mo ... | 1979 | 375081 |
| purification of some glycoside hydrolases by affinity chromatography. | two glycoproteins have been isolated from the cell walls of baker's yeast. one is a glucan-protein complex which has been partially characterised as having a branched carbohydrate structure composed of chains of (1 leads to 3)-linked beta-d-glucosyl residues, some of which are attached by (1 leads to 6)-linkages to the main chain. immobilization of this glycoprotein was achieved by covalent attachment to sepharose, and the product was used to isolate a number of (1 leads to 3)-beta-d-glucan hydr ... | 1977 | 409497 |
| [preparation and properties of protoplasts from saccharomyces vini k-42]. | lytic enzymes from the edible snail--helix pomatia--were used to obtain protoplasts of yeast saccharomyces vini k-42. the yeast grown in aerobic conditions proved more sensitive. the resultant protoplasts oxidized glucose and sucrose and did not oxidize maltose. | 1979 | 515001 |
| [receptors of human red blood cells for anti-tah and anti-ahp in the course of intravascular aging]. | human o-erythrocytes aged in situ do not expose free receptors for anti-t agglutinin of arachis hypogea. new receptors for concentrated anti-a agglutinin of helix pomatia are manifested but are lost again in the course of further ageing of the cells in situ. a remasking of exposed receptors for anti-tah and anti-ahp byautologous, strongly binding globulins is supposed. these globulins could constitute the decisive signal for the autologous phagocytosis of senescent red blood cells. | 1979 | 525143 |
| [lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)]. | lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ... | 1979 | 547035 |
| studies on the cell-free biosynthesis of beta-lactam antibiotics. | cell walls of cephalosporium acremonium mycelia were lysed by enzyme preparations from either helix pomatia (snail) digestive juice or cytophaga. the yield of protoplasts depended on the lytic-enzyme preparation and the age of the culture, and it increased after the mycelia were pretreated with dithiothreitol. a cell-free preparation, obtained by osmotic lysis of protoplasts, synthesized labelled penicillin n from l-[14c]valine. approx. 0.03-0.06% of the amino acid was incorporated into penicill ... | 1977 | 559491 |
| carbohydrate binding specificity of four n-acetyl-d-galactosamine- "specific" lectins: helix pomatia a hemagglutinin, soy bean agglutinin, lima bean lectin, and dolichos biflorus lectin. | | 1977 | 560855 |
| studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels. | a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ... | 1978 | 563738 |
| [fractional protein makeup of candida utilis yeast protoplasts in the process of their growth and development]. | candida utilis ibfmy-405 was grown in a synthetic medium with glucose. cells taken at the logarithmic phase of growth were studied. the cells were treated with the enzyme from helix pomatia to prepare protoplasts which were separated by differential centrifugation into groups according to their size. three protein fractions were isolated from each group and the amino acid composition of the proteins was determined. proteins of the first fraction (cytoplasmic) prevailed in all of the protoplast g ... | 1979 | 571510 |
| a versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations. | a procedure for cell fractionation using lectin-affinity chromatography is described. it consists of a single affinity adsorbent, hog gastric mucin blood group a+h substance covalently coupled to sephadex or sepharose, to which lectins of various specificities can bind. the complex formed, lectin in equilibrium hog a+h substance-sephadex, then serves as an affinity probe for isolating and fractionating cells. the lectins from ulex europaeus, lotus tetragonolobus, helix pomatia, dolichos biflorus ... | 1979 | 573269 |
| opsonizing effect of serum and albumin gland extracts on the elimination of human erythrocytes from the circulation of helix pomatia. | | 1978 | 632614 |
| substrates for the assay of alpha-l-iduronidase. | procedures are described for the preparation of two disaccharides, 4-o-alpha-l-iduronosyl-2,5-anhydro[3h]mannitol and 3-o-alpha-l-iduronosyl-2,5-anhydro[3h]-talitol, from heparin and dermatan sulfate, respectively. these disaccharides lend themselves to an easy assay of alpha-l-iduronidase which is based on the fractionation of the liberated neutral anhydro[3h]mannitol or anhydro[3h]talitol from the unreacted substrate by adsorption of the latter to dowex 1. investigation of the reaction conditi ... | 1978 | 709885 |
| a sulphate metabolite of trimethoprim in goats and pigs. | 1. urine from goats and pigs after intravenous administration of [14c]trimethoprim contained an unidentified fraction of 'other water-soluble metabolites'. this fraction accounts for 56% of the administered dose in goat urine and 65% in pig urine. 2. a sulphate metabolite comprising the major part of the latter fraction in both goat and pig urine was isolated by gel filtration and paper chromatography and identified by spectroscopic methods as 2,4-diamino-5-(3,4-dimethoxy-5-sulphooxybenzyl)pyrim ... | 1978 | 726512 |
| enrichment of helix pomatia (hp) lectin binding variant from the ta3st mouse ascites tumor by repeated column selection. | | 1978 | 738331 |
| [decomposition of steroids during incubation with beta-glucuronidase and during storage of urine]. | androsterone, etiocholanolone, pregnanetriol, dehydroepiandrosterone, pregnanediol, tetrahydrocortisol, 5-pregnenolone and 11-beta-oh-androsterone were incubated with beta-glucuronidase preparations (helix pomatia, bovine liver and e. coli) for 96 hrs at 37 degrees c. after extraction and silylation they were gas-chromatographed. the first 3 steroids were left practically intact. the least decomposition of the last 5 steroids occurred with the liver enzyme. testosterone and 11-ketoandrosterone w ... | 1975 | 765125 |
| the interaction of nonmitogenic and mitogenic lectins with t lymphocytes: association of cellular receptor sites. | the relationship between the surface receptors on neuraminidase-treated human blood lymphocytes for the mitogenic lectins phaseolus vulgaris leukoagglutinin (la), concanavalin a (con a) and soy bean agglutinin (sba) and the non-mitogenic lectin helix pomatia a hemagglutinin (hp) was investigated. two different techniques, co-capping with different fluorochrome-labeled lectins and cell binding-inhibition experiments with 125i-labeled lectins, were used. the results demonstrated that the nonmitoge ... | 1976 | 772792 |
| an ultrastructural analysis of protoplast-spheroplast induction in cryptococcus neoformans. | protoplasts-spheroplasts of a human isolate of cryptococcus neoformans were prepared using the gut enzyme of helix pomatia. the induction process, as studied by transmission electron microscopy, occurred in two stages. early in the induction process, protoplasts-spheroplasts emerged from whole cells through a break in the cell wall-capsule envelope. later, a gradual dissolution of the entire cell wall occurred releasing the intact protoplast-spheroplast. a comparison of protoplasts-spheroplasts ... | 1976 | 788868 |
| [use of an agglutinin from the albumin gland of the edible snail for immunoelectron microscopic localization of cell membrane antigens with terminal n-acetyl-d-galactosamine]. | | 1975 | 829939 |
| high-performance liquid chromatographic separation and photometric detection of phospholipids. | a rapid and efficient method for the separation of (phospho)lipids by high-performance liquid chromatography using n-hexane-2-propanol-water mixtures as the solvent system is described. the lipid separation occurs on silica gel columns and the individual components are monitored directly by uv absorption at 206 nm. of a total lipid extract from erythrocytes as well as suboesophageal ganglia of the snail helix pomatia, a complete separation is achieved of cholesterol, phosphatidic acid, phosphati ... | 1977 | 914946 |
| alkali-labile oligosaccharides from glycoproteins of different erythrocyte and milk fat globule membranes. | phenol extraction of horse, sheep, cow, pig and human erythrocyte membranes and human milk fat globule membranes gave glycoprotein fractions, all of which were shown by gas chromatography to contain the reduced disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosaminital after treatment with alkaline borohydride. cow and pig erythrocyte membrane glycoproteins were found however to contain much lower amounts than the erythrocyte membrane glycoproteins of the other species tested. after gel fi ... | 1976 | 963062 |
| determination of arginine in the reactive site of proteinase inhibitors by selective and reversible derivatization of the arginine side chain. | reversible and selective modification of the guanidino group by 1,2-cyclohexanedione or 2,3-butanedione in borate buffer was applied to identify arginine residues in the reactive sites of proteinase inhibitors. the ability of the method to distinguish between arginine and lysine residues was examined. trypsin inhibitors with arginine at the reactive site were almost completely inactivated within 2 h, e.g. the low-molecular weight inhibitors from soybeans (kunitz), peanuts and porcine seminal pla ... | 1976 | 964925 |
| [direct action of angiotensin ii on the central neurons]. | reactions of the nervous cells in the somatosensory and visual regions of the brain cortex and the frontal hypothalamus in rabbits, as well as of the isolated nervous peripharyngeal ring of the helix pomatia to the microionophoretic application of angiotensin ii (a-ii) was studied. reactions of the neurons in the rabbit brain to a-ii displayed an increase in the spike frequency depending on the quantity of the agent applied. reactions of the frontal hypothalamus neurons showed a lower threshold ... | 1976 | 1026279 |
| trypsin-kallikrein isoinhibitor k (type kunitz) from snails (helix pomatia). purification and characterization. | a basic proteinase inhibitor, isoinhibitor k, was purified by se-sephadex c-25 column chromatography from the mixture of acid-stable and heat-stable isoinhibitors of the snail (helix pomatia). isoinhibitor k is homogeneous in polyacrylamide gel, cellulose acetate and polyacrylamide-dodecylsulfate electrophoresis. from the electrophoretic mobility in dodecylsulfate-polyacrylamide gel and apparent molecular weight of 6500 +/- 200 was estimated. from the amino acid composition the inhibitor consist ... | 1975 | 1081050 |
| receptors for helix pomatia a haemagglutinin on leukaemic lymphocytes from patients with chronic lymphocytic leukaemia (cll). | blood lymphocytes from thirteen patients with cll were studied for surface-bound ig (sig), fc receptors (ea rosettes), receptors for sheep erythrocytes (e rosettes) and receptors for helix pomatia a haemagglutinin (hp), a carbohydrate-binding protein with specificity for n-acetyl-d-galactosamine and related sugars. fluorescein-labelled hp binds to subpopulations of human peripheral blood lymphocytes (pbl) treated with neuraminidase. in normal peripheral blood, hp binds to the t-lymphocytes while ... | 1976 | 1086751 |
| fractionation of human blood lymphocytes on helix pomatia a haemagglutinin coupled to sepharose beads. | treatment of human blood lymphocytes with neuraminidase has previously been shown to uncover receptors for the a haemagglutinin of the snail helix pomatia (hp). neuraminidase-treated lymphocytes were now fractionated on columns charged with large sepharose particles to which hp had been coupled covalently. hp-receptor negative (hp-) lymphocytes passed the columns while hp-receptor positive (hp+) lymphocytes were retained. the latter cells were eluted by addition of the competitive hapten n-acety ... | 1976 | 1088709 |
| concanavalin a and other lectins in the study of tumor cell surface organization. | cell surface structures of two mouse ascites tumors were studied using lectins. the tumors are sublines of the spontaneous mammary adenocarcinoma ta3 in strain a, differing in two main characteristics. subline ta3-st grows only in syngeneic mice and has high expression of h-2 antigens. subline ta3-ha, in contrast, proliferates in all mouse strains, and has low amounts of exposed h-2 antigens. concanavalin a (con a), phytohemagglutinin (pha) and helix pomatia anti a hemagglutinin (hp) were used i ... | 1975 | 1098413 |
| [immunoelectronmicroscopic localization of cell wall antigens in streptococci. ii. localization of the group-specific polysaccharide of group c streptococci with ferritin- and peroxidase-labelled helix pomatia-agglutinin (author's transl)]. | the carbohydrate of the cell wall of group c streptococci is one of the best known group-specific streptococcal antigens with respect to its chemical structure. in the present paper, the ultrastructural location of the carbohydrate was investigated by means of immunoelectronmicroscopic techniques. besides group-specific antibodies, the specific binding of an agglutinin (protectin anti-ahp) of the edible snail (helix pomatia) to structures with terminal n-acetylgalactosamine was used to demonstra ... | 1975 | 1154918 |
| [production of candida lipolytica protoplasts]. | optimal conditions were found for the production and isolation of the protoplasts of candida lipolytica. the maximum amount of the protoplasts was produced after 90 minutes of the incubation with a crude preparation of the enzyme from helix pomatia (100 mg/g wet biomass). longer incubation results in lysis of the protoplasts and structural damages of the intracellular components. the yield of the protoplasts does not depend on the nature of stabilizing agent. a decrease in the stabilizer concent ... | 1975 | 1160627 |
| [spheroplasts of torulopsis candida yeasts and their oxidizing activity]. | the paper describes the conditions in which the spheroplasts of the yeast torulopsis candida ibfm-y-127 with a high respiration rate can be isolated. the preliminary incubation of the cells with sh-reagents has to be carried out in a buffer without an osmotic stabilizer, and the incubation in a medium containing 0.6 m kcl, 0.1 m mgso4, 0.1 mkh2po4, ph 5.2. in these conditions the cells are incubated with the enzyme from helix pomatia during 15 to 20 minutes, and the yield of the spheroplasts is ... | 1975 | 1172174 |
| [the enzymatic hydrolysis of cell wall applied to yeasts classification (author's transl)]. | the rigid structure of yeast cell wall may be a taxonomic criterion. using the helix pomatia juice (with mercaptoethylamine hydrochloride) as a polyenzymatic system, we have always obtained protoplasts from ascomycetous yeasts and never from basidiomycetous yeasts tested. then, we have applied this enzymatic analysis to the fungi imperfecti yeasts and, according to this test, separated genus looking like basidiomycetous behaviour: trichosporon, pityrosporum, rhodotorula, cryptococcus and non fer ... | 1975 | 1190637 |
| the lack of mitogenic response of neuraminidase-treated and untreated human blood lymphocytes to divalent, hexavalent, or insoluble helix pomatia a hemagglutinin. | | 1975 | 1193182 |
| [neuraminidase induced hemolytic anemia. experimental and clinical observations (author's transl)]. | recently, increasing attention has been focussed on the in vivo action of neuraminidase as possible pathogenetical factor of hemolytic anemia and even hemolytic-uremic syndrome. neuraminidase action in red cell membranes results in the release of neuraminic acid, and thereby the uncovering of previously hidden receptors, socalled cryptantigens. with special reference to the phythemagglutinin anti-tah from the peanut (arachis hypogae) and the agglutinin anti-ahp from the albumin gland of the smal ... | 1976 | 1246237 |
| modulation of voltage-activated ion currents on identified neurons of helix pomatia l. by interleukin-1. | 1. the effect of interleukin-1 (il-1) was studied on voltage-activated ion currents of the identified central neurons of helix pomatia l. using a two-microelectrode voltage clamp. the voltage-activated inward current (ica) was decreased, whereas the outward current (i(net) k) was increased by il-1. 2. il-1 affects both the transient and the delayed rectifying potassium currents. the il-1 modulatory effect on the voltage-activated ion currents was voltage and dose dependent. the threshold concent ... | 1992 | 1281756 |
| comparative study of prostatic carcinoma bone metastasis among japanese in japan and japanese americans and whites in hawaii. | metastatic prostate carcinomas in autopsy cases from three populations 49 cases of indigenous japanese, 29 cases of japanese americans and 14 from whites in hawaii) were compared in terms of their clinicopathological, immunohistochemical (tenascin and ras p21) and lectin binding (helix pomatia antigen, hpa) properties. only the clinicopathological features were analyzed in the cases of whites in hawaii. the results indicate that poorly differentiated carcinoma is less common, whereas distant met ... | 1992 | 1283503 |
| lectin histochemistry and alkaline phosphatase activity in the pia mater vessels of spontaneously hypertensive rats (shr). | some lectins were used to study the localization of sugar residues on the endothelial cell surface in the pia mater blood vessels of control (wky) and hypertensive rats (shr). the lectins tested recognized the following residues: beta-d-galactosyl (ricinus communis agglutinin 120, rca-1), alpha-l-fucosyl (ulex europaeus agglutinin, uea-1), n-acetylglucosaminyl and sialyl (wheat germ agglutinin, wga), n-glycolyl-neuraminic acid (limax flavus agglutinin, lfa), and n-acetyl-d-galactosaminyl (helix ... | 1992 | 1297933 |
| duck lymphocytes--v. transformation responses to phorbol ester and calcium ionophore. | 1. lymphocytes purified from duck blood and spleen were cultured in the presence of phorbol 12-myristate 13-acetate (pma) and the calcium ionophore a23187. stimulation was assessed by the incorporation of [3h]thymidine after 3 days' culture. 2. pma stimulated over a wide range of concentrations, with maximum stimulation at final concentrations of 5 x 10(-7)-5 x 10(-8) m/litre. a23187 was effective in the range 5 x 10(-6)-5 x 10(-7) m/litre and also, in some experiments using spleen lymphocytes, ... | 1992 | 1354147 |
| quantitative changes of ricinus communis agglutinin i and helix pomatia lectin binding sites in the acrosome of rat spermatozoa during epididymal transit. | during passage through the epididymis, spermatozoa undergo a number of changes which result in their acquisition of fertility and motility. some of the changes that occur include loss of the cytoplasmic droplet and changes in sperm morphology, metabolism and properties of the nucleus and plasma membrane. changes have also been reported in the acrosomic system of mammalian spermatozoa during their transit through the epididymis. in the present study, the quantitative changes of the glycoconjugate ... | 1992 | 1385371 |
| expression of glycoconjugates in the human fetal cochlea. | the distribution of glycoconjugates in the human fetal cochlea was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). the tectorial membrane (tm) in the 11- and 15-week-old human fetuses was labelled with wga, apa, rca120 and cona, but not with uea-i and hpa. in the 19-week-old fetuses, the reaction of the tm ... | 1992 | 1442009 |
| analysis by a plaque assay of igg- or igm- dependent cytolytic lymphocytes in human blood. | when monolayers of bovine erythrocytes (eb) were exposed to purified human blood lymphocytes and either igg or igm fractions of rabbit anti-eb serum, clear zones (plaques) appeared when eb had been lysed by antibody-dependent effector cells (k cells). igg-dependent plaque formation was complete by 20 h of incubation, while the igm-dependent reaction required 40 h. the estimated minimal numbers of plaque forming cells (pfc) were 5.6% (igg) and 2.0% (igm) of the added lymphocytes. inhibition exper ... | 1976 | 993729 |
| radioimmunological determination of urinary tetrahydroaldosterone. | a method is presented for radioimmunological determination of 3alpha, 5beta-tetrahydroaldosterone. it is based upon the reactivity of this steroid with an antiserum induced by the 3-carboxymethyloxime of 18, 21-aldosterone diacetate conjugated with bovine serum albumin. one hundred microliters of urine enzymatically hydrolyzed with an helix pomatia preparation, containing tritiated tetrahydroaldosterone for the yield calculation, were extracted with dichloromethane and chromatographed on a small ... | 1977 | 910248 |
| occurrence of a blood group a-like substance in eggs of the prosobranch snail pomacea canaliculata. | in the eggs of the prosobranch snails pomacea canaliculata and pomacea insularum a blood group a-like substance has been detected by anti-a from the snails helix pomatia, helix aspersa and cepaea nemoralis. | 1976 | 815102 |
| [characterization of streptococcus uberis with special consideration of supposed pathogenicity factors]. | streptococcus uberis, as one of the principal causes of bovine streptococcal mastitis, has been characterized serologically and biochemically. serological grouping of s. uberis revealed polysaccharide antigens of groups e, g, p and u. the biochemical properties of s. uberis, determined with the strep-zym identification system, differed clearly from those of s. agalactiae and s. dysgalactiae. some cultures of s. uberis produced the enzymes hyaluronidase and neuraminidase. in addition s. uberis pa ... | 1992 | 1497584 |
| immuno-chemical studies on the alkali-labile carbohydrate chains of human serum glycoproteins. | human serum glycoproteins can be classified into those containing n-acetyl-d-galactosamine and into those lacking this hexosamine. the n-acetyl-d-galactosamine-containing serum glycoproteins have alkali-labile chains containing this hexosamine linked o-glycosidically to hydroxy amino acids. these alkali-labile chains can be demonstrated in neuraminic acid free serum glycoproteins by gas liquid chromatography and by using precipitating lectins from invertebrates and plants. they are represented b ... | 1979 | 762504 |
| the use of immobilized lectins in the separation of staphylococcus aureus, escherichia coli, listeria and salmonella spp. from pure cultures and foods. | lectins from helix pomatia, canavalia ensiformis, agaricus bisporus and triticum vulgaris agglutinated cultures of staphylococcus aureus, escherichia coli, listeria and salmonella spp. this agglutination was specific as it was inhibited (except with a. bisporus lectin) by the competing sugar substrates. the ability of three of these lectins, immobilized on a variety of supports, to separate these micro-organisms from pure cultures was investigated. immobilization of the lectins on magnetic micro ... | 1992 | 1512177 |
| [characteristics of obtaining protoplasts from 2 strains of tolypocladium inflatum subsp. blastosporum]. | the optimal conditions for preparing protoplasts with high yields by using the cells of two (low and high potent) isogenic cyclosporine-producing tolypocladium strains were developed. a specific medium containing 0.5 per cent yeast autolysate (by dry weight) and 3 per cent glucose was used. when grown on this medium the cells of the highly potent strain 847 acquired a yeast-like shape. high yields of protoplasts prepared from the low potent strain 43 mycelium were obtained via prior incubation w ... | 1992 | 1514857 |
| histochemical localization of glycoconjugates in the developing endolymphatic sac and vestibular end organs of the mouse. | the distribution of glycoconjugates in the endolymphatic sac (es) and vestibular end organs from the 12th gestational day (gd) in the developing mouse to the 6th day after birth was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 13th and 15th gd sections, the luminal contents in the fetal es were st ... | 1992 | 1518658 |
| agglutination of bordetella species by lectins. | phase i cells of bordetella pertussis but not those of b. parapertussis, b. bronchiseptica or b. avium were agglutinated by limulus polyphemus lectin. most strains of b. pertussis but not those of the other species were also agglutinated by helix pomatia lectin. in precipitation reactions between lectins and purified bordetella lipopolysaccharide (lps) preparations a similar pattern occurred. lectin agglutination provides a rapid presumptive method for the differentiation of b. pertussis from b. ... | 1992 | 1555759 |
| resistance of hamster bronchiolar epithelium to neutrophil elastase: investigation by cell surface lectin cytochemistry. | an intratracheal instillation of human neutrophil elastase (hne) causes accumulation of an excess number of secretory granules in the epithelial secretory cells lining the hamster bronchus. this chronic lesion, which we refer to as secretory cell metaplasia (scm), is not seen in the trachea or bronchioles. because luminal cell surface lectin binding is much higher in the trachea than in the bronchus, we concluded that tracheal resistance may be due to a protective glycoconjugate coat. in the pre ... | 1992 | 1572319 |
| interaction of peanut agglutinin with human lymphocytes. binding properties and topology of the receptor site. | the relationship between the mitogenic lectin pna and other mitogenic and non-mitogenic lectins was investigated. pna labelled with 125i was found to bind equally well to t and b lymphocytes, after neuraminidase treatment, with 3.88 times 10(6) and 3.73 times 10(6) binding sites respectively. only the t cell fraction was stimulated, however, and only after neuraminidase treatment. preincubation of neuraminidase-treated cells with non-mitogenic lectins and antiserum which appeared to bind to the ... | 1978 | 726549 |
| a 1h nuclear-magnetic-resonance study of the solution conformation of the isoinhibitor k from helix pomatia. | the isoinhibitor k from helix pomatia was investigated by high-resolution 1h nuclear magnetic resonance at 360 mhz. detailed studies of the labile protons and the resonances of the aromatic residues indicated extensive homologies between the spatial structures of the snail inhibitor, the basic pancreatic trypsin inhibitor (kunitz) from bovine tissue and the cos colostrum trypsin inhibitor. comparison of these three homologous inhibitors indicated that the overall flexibility of the globular prot ... | 1978 | 710398 |
| subdomains of the rough endoplasmic reticulum in colon goblet cells of the rat: lectin-cytochemical characterization. | using lectin binding, we characterized subdomains of the rough endoplasmic reticulum (rer) in goblet cells of the rat colon. in this cell type, special rer regions can be differentiated on the basis of their content of low electron density and dilated cisternal spaces in conventional transmission electron microscopic preparations. the fine fibrillar content of these cisternal regions demonstrated high-affinity binding with lectins from wheat germ, helix pomatia, griffonia simplicifolia i-a4 and ... | 1992 | 1607641 |
| the hamster transferrin receptor contains ser/thr-linked oligosaccharides: use of a lectin-resistant cho cell line to identify glycoproteins containing these linkages. | we recently reported that the human transferrin receptor (tfr) contains o-linked galnac residues [1]. to investigate whether this modification is shared by transferrin receptors in other mammals, we investigated the glycosylation of tfr in hamster cells. to facilitate our analysis the lectin-resistant chinese hamster ovary (cho) cell line lec8 was used. these cells are unable to galactosylate glycoproteins, resulting in truncation of the ser/thr-linked oligosaccharides to a single residue of ter ... | 1992 | 1640050 |
| electron microscopic detection of the cryptantigen ahumhp (friedenreich antigen) on human erythrocytes by means of gold-labelled agglutinin from helix pomatia. | using the gold-labelled agglutinin anti-ahp from helix pomatia (edible snail) the ultrastructural site and distribution of the cryptantigen (friedenreich antigen) ahumhp was revealed on neuraminidase treated human o and b erythrocytes. on a1 and a2 cells the antigen was demonstrated simultaneously with the blood group antigen a by labelling the cells with anti-ahp-ferritin followed by removing the neuraminic acid and labelling with anti-ahp-gold. the cryptantigen is focally distributed on the ce ... | 1977 | 602347 |
| 3,3-bis-(4-hydroxyphenyl)-7-methyl-2-indolinone (bhmi), the active metabolite of the laxative sulisatin. | the disodium salt of the sulphuric diester of 3,3-bis-(4-hydroxyphenyl)-7-methyl-2-indolinone (sodium sulisatin, laxitex), a synthetic laxative with two phenolic groups esterified with sulfate, has been studied in order to find out if its laxative properties may be attributed to the unaltered compound or to its diphenolic derivative bhmi. we first studied the effect of homogenates of the gastrointestinal tract of rats and of rat cecal content of the hydrolysis of sulfate ester bonds of sulisatin ... | 1979 | 583222 |
| lectin binding patterns to terminal sugars of rat lung alveolar epithelial cells. | we used post-embedding cytochemical techniques to investigate the lectin binding profiles of rat lung alveolar epithelial cells. sections from rat lung embedded in the hydrophilic resin lowicryl k4m were incubated either directly with a lectin-gold complex or with an unlabeled lectin followed by a specific glycoprotein-gold complex. the binding patterns of the five lectins used could be divided into three categories according to their reactivity with alveolar epithelial cells: (a) the limax flav ... | 1990 | 1688898 |
| [determination of lectin receptors on the surface membranes of lymphoid cells]. | the authors describe a method for the detection of carbohydrate determinants contained by the cellular surface membrane glycosylated biopolymers, based on the use of horse radish peroxidase-conjugated arachis, helix pomatia, ricinus communis, lens, glycine max, sophora japonica lectins. the pattern of lectin receptor distribution on normal human blood lymphocytes, tonsillar lymphoid cells, and in some forms of leukemia and lymphoma is shown. the authors consider it essential that a lectin kit be ... | 1990 | 1692358 |
| immunochemical detection of the thomsen-friedenreich antigen (t-antigen) on platelet plasma membranes. | platelet plasma membranes were found to possess the disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosamine which was measured by gas chromatography after release by alkaline borohyride treatment and desialylation. immunological evidence using the specific lectins from arachis hypogoea and agaricus bisporus and an anti-t serum confirmed the presence of this disaccharide, the immunodominant group of the thomsen-friedenreich antigen (t-antigen). this receptor was only found after prior neura ... | 1978 | 580492 |
| changes in glycoconjugates revealed by lectin staining in the developing airways of syrian golden hamsters. | lectin binding was studied in the developing airways of syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). the trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: triticum vulgare (wga), dolichos biflorus (dba), helix pomatia (hpa), maclura pomifera (mpa), griffonia simplicifolia i-b4 (gsa i- ... | 1990 | 1700650 |
| cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4. | we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ... | 1991 | 1701188 |
| difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the abh antigens in human tissues. | twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-a, -b and -h antibodies for detecting the corresponding antigens in selected human tissues. although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. helix asparsa agglutinin (haa), helix pomatia agglutinin (hpa) and monoclonal anti-a antibody recognized a antigens in the mucous cells of sa ... | 1990 | 1705925 |
| synaptic and hormonal modulation of a neuronal oscillator: a search for molecular mechanisms. | 1. the central ganglia of a number of gastropod molluscs (including the marine snail aplysia californica and the terrestrial snail helix pomatia) contain neurones which exhibit endogenous patterns of oscillatory activity. 2. this oscillatory activity can be modulated for long periods of time by synaptic and hormonal stimulation. 3. stimulation of appropriate pre-synaptic nerves causes long-lasting hyperpolarization in these neurones, with complete abolition of oscillatory activity. this synaptic ... | 1979 | 512575 |
| heterogeneity and mechanism of action of human natural killer lymphocytes: differential distribution of receptors for helix pomatia haemagglutinin (hp receptors). | neuraminidase-treated lymphocytes from the peripheral blood of normal human donors were fractionated on columns charged with helix pomatia haemagglutinin (hp) coupled to sepharose 4b. while lymphocytes lacking hp receptors (hp-) passed directly through the column (fraction i), lymphocytes with hp receptors (hp+) were subsequently eluted in two distinct fractions with two different concentrations of the competitive hapten n-acetyl-d-galactosamine (fraction ii and iii, respectively). the natural c ... | 1979 | 493880 |
| the isolectins of helix pomatia. separation by isoelectric focusing and preliminary characterization. | helix pomatia lectin was fractionated into several (at least 12) components by isolectric focusing in pharmalytetm. eight of these were recovered from a preparative column experiment and shown to be essentially pure by analytical electrofocusing in polyacrylamide gel slabs. the lectin components had haemagglutinating activity against human group a erythrocytes and similar molecular weights of 80 000 as determined vidual components showed slight variations in composition. the components are consi ... | 1979 | 465535 |
| presence of terminal n-acetylgalactosamine residues in subregions of the endoplasmic reticulum is influenced by cell differentiation in culture. | using helix pomatia lectin as a specific probe for terminal, nonreducing n-acetylgalactosamine residues, glycoprotein precursors bearing newly initiated o-linked oligosaccharides have been localized in the lumen of the endoplasmic reticulum and cis-golgi cisternae. this pattern contrasts with the detection of the terminal disaccharide galactose beta-1,3-n-acetylgalactosamine by arachis hypogaea lectin in middle and trans-golgi compartments, which are considered elongation sites for o-glycosylati ... | 1991 | 1748669 |
| carbohydrates in the guinea pig stria vascularis demonstrated with lectin-gold techniques. | soybean agglutinin (sba), helix pomatia agglutinin (hpa), ricinus communis agglutinin-ii (rca-ii), limax flavus agglutinin (lfa) and wheat germ agglutinin (wga) were employed to determine the localization of specific carbohydrates on thin sections of lowicryl k4m embedded guinea pig striae vasculares using the lectin-gold and glycoprotein-gold techniques. sba, hpa and rca-ii gold labeling was observed in many of the cytoplasmic vesicles, endosomes and apical tubules located in the supranuclear r ... | 1991 | 1763624 |
| receptors for helix pomatia a haemagglutinin (hp) on a subpopulation of human b cells. | receptors for helix pomatia a haemagglutinin (hp) have earlier been found on neuramidinase treated t-lymphocytes in human peripheral blood. in contrast, the majority of the b-lymphocytes, characterized by surface bound igm and/or igd(sig)lack these receptors. double marker experiments with fluorochrome labelled reagents have now shown that a minor fraction (3--24%) of the igm/d bearing lymphocytes in normal human blood also have hp-receptors. these hp+ b-cells constitute approximately 1% of the ... | 1978 | 349677 |
| biochemical and serological properties of streptococcus uberis. | the strep-zym identification system, a combination of 23 enzymatic tests, allowed a rapid biochemical characterization of streptococcus uberis. the biochemical profiles of the s. uberis cultures clearly differed from those of s. agalactiae and s. dysgalactiae. serological grouping of s. uberis revealed polysaccharide antigens of groups e, g, p and u. some cultures of s. uberis demonstrated camp-like synergistic hemolytic activities on sheep blood agar and reacted specifically with the lectins of ... | 1991 | 1796686 |
| a new rat lymphocyte surface marker: characterization and separation of cells with receptors for helix pomatia hemagglutinin. | thirty-two percent of neuraminidase-treated da rat spleen lymphocytes and 48% of lymph node lymphocytes possess receptors for helix pomatia hemagglutinin (hp). moreover, these hp-receptor-bearing cells can be separated from b cells by affinity chromatography on hp-sepharose columns. the virtual absence of immunoglobulin (ig) receptors and the close correlation with reported t-cell content of these lymphoid tissues suggest that hp-receptor lymphocytes are probably t cells and that hp may provide ... | 1977 | 322264 |
| defining the specificity of anti-a/b igm/g antibodies with different antigens and lectins. | the specificity of anti-histo blood group a/b antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mabs) or by the antigens present on various intestinal bacteria (for polyclonal human igm and/or igg, phabs). absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use a/b substances of human, animal and synthetic origin as well as known reactivities of mabs or lectins a ... | 1991 | 1862641 |
| a 58-kda resident protein of the cis golgi cisterna is not terminally glycosylated. | a 58-kda golgi protein (gp58) was previously identified and found to be concentrated in cis golgi cisternae in several cell types (saraste, j., palade, g.e., and farquhar, m.g. (1987) j. cell biol. 105, 2021-2029). in this study the protein was partially purified from rat pancreas and mouse myeloma cells in order to characterize its oligosaccharides. it migrated on sodium dodecyl sulfate-polyacrylamide gels as a 57-58-kda doublet under reducing conditions or as a single approximately 116-kda ban ... | 1991 | 1894639 |
| a new surface marker on mouse natural killer cells: receptors for helix pomatia a hemagglutinin. | natural killer (nk) cells in normal mouse spleen have so far been found to lack conventional cell surface markers. recently, neuraminidase treatment has been shown to uncover receptors for the a hemagglutinin of the snail helix pomatia (hp) on populations (preferentially t cells) of human blood lymphocytes. we have used hp coupled to sepharose in columns to fractionate mouse spleen cells. fractions consisted of passed cells (fraction i) and cells eluted with the competitive hapten n-acetyl-d-gal ... | 1978 | 309820 |
| glycoconjugates in the human fetal endolymphatic sac as detected by lectins. | the distribution of glycoconjugates in the 11 to 16 weeks old human fetal endolymphatic sac (es) was analyzed using six biotinylated lectins; wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 11 week old human fetus, fluorescent reactions with wga, apa, rca120 and cona were detected in the es. there was almost no reaction with hpa and uea-i. ... | 1991 | 1919336 |
| species identification and characterization of an acanthamoeba strain from human cornea. | the isoenzyme pattern of an acanthamoeba, stock h-1, isolated from a patient with keratitis (krankenhaus heidberg, hamburg) was compared with that of two strains of a. quina-a. lugdunensis (302-2, 312-1), two stocks of a. lenticulata (45, 89-1) and one strain of a. rhysodes (302-1). the isolated stock showed glucose-6-phosphate dehydrogenase (g-6-pdh), beta-hydroxybutyric dehydrogenase (beta-hbdh), alcohol dehydrogenase (adh) and superoxide dismutase (sod) isoenzyme patterns similar to those of ... | 1991 | 1924251 |
| helix pomatia a hemagglutinin: selectivity of binding to lymphocyte surface glycoproteins on t cells and certain b cells. | human and mouse lymphocytes were surface-labeled by lactoperoxidase-catalyzed iodination, or by galactose oxidase oxidation followed by reduction with tritiated sodium borohydride. the labeled cells were lysed with nonidet p-40. proteins binding to helix pomatia a hemagglutinin (hp) were isolated by affinity chromatography on hp-sepharose and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography. a major cell surface glycoprotein (apparent mol. wt. 150 000, us ... | 1978 | 309819 |
| class-specific antibody titres (elisa) against the primary immunogen helix pomatia haemocyanin (hph) in man. | using the indirect elisa technique, igm, igg and iga antibody titres against the primary test immunogen helix pomatia haemocyanin (hph) could be measured. all twenty-seven normal volunteers (age range 26-74 years) developed maximal or high igm, igg and iga antibody titres 2 weeks after primary immunization with 1.0 mg hph subcutaneously. lower dose immunization resulted in submaximal responses. titre kinetics in the three ig classes correlated in height and time per person. there was no signific ... | 1978 | 80296 |
| aluminofluorides and beryllofluorides as inhibitors of sulphatases. analogues of hydrogen sulphate? | the inhibition of fluoride of sulphatase a from ox liver and of the sulphatases of helix pomatia and aspergillus oryzae is decreased by edta and increased by al3+ or be2+, implicating aluminofluorides and beryllofluorides in the reaction. the inhibition, which is reversible, takes several minutes to develop fully and, at least for the sulphatase of h. pomatia, is of a non-linear mixed competitive-non-competitive type. it is suggested that the aluminofluorides and beryllofluorides are acting as a ... | 1991 | 1953634 |
| the adsorption of bacteria to immobilized lectins. | the agglutination of a selection of bacteria by some lectins was examined. the lectin from codium fragile agglutinated seven strains of salmonella typhimurium. the lectin from helix pomatia agglutinated eight of 12 strains of listeria monocytogenes and a further two strains gave a weak agglutination reaction. helix pomatia lectin conjugated to magnetic microspheres enabled the adsorption of l. monocytogenes from suspension with subsequent elution by the competing ligand n-acetyl galactosamine. a ... | 1991 | 1955420 |
| expression of blood group-related antigens and helix pomatia agglutinin in malignant pleural mesothelioma and pulmonary adenocarcinoma. | in an attempt to differentiate malignant pleural mesothelioma from pulmonary adenocarcinoma by histochemical and immunohistochemical means, the glycoconjugate profiles of five reactive mesothelial lesions, 29 mesotheliomas (20 epithelial, three biphasic, and six fibrous types), and 38 well-differentiated pulmonary adenocarcinomas (34 papillary, two tubular, and two bronchioloalveolar types) were tested with abh blood group-related antigens (bgr-ag) antibody and helix pomatia agglutinin (hpa) whi ... | 1991 | 2001875 |
| improved hydrolysis of urinary 17-hydroxycorticosteroid glucuronides with beta-glucuronidase from helix pomatia, on adding sodium sulfate. | sodium sulfate increases the hydrolysis of urinary 17-hydroxycorticosteroid glucuronides with beta-glucuronidase preparations derived from helix pomatia because it removes the inhibitory activity of urinary high-molecular-weight substances. for maximum hydrolysis of urinary 17-hydroxycorticosteroid glucuronides, the hydrolysis 5 ml of urine, 0.5 ml of 2 mol/liter acetate buffer (ph 5.0) should be conducted in the presence of sodium sulfate (final concentration: 80 g/liter) with (a) 600 fishman u ... | 1977 | 21763 |
| lectin histochemistry of developing submandibular glands of fetal syrian golden hamsters. | lectin binding was studied in the developing submandibular glands of fetal syrian golden hamsters (mesocricetus auratus) from gestational day 12 to 16 (the day of birth). the fetuses were fixed, embedded in paraffin, sectioned and stained with nine lectin-horseradish peroxidase conjugates: concanavalin a (con a), wheat germ agglutinin (wga), dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), maclura pomifera agglutinin (mpa). griffonia simplicifolia agglutinin i-b4 (gsa i-b4), p ... | 1991 | 2035849 |
| glycoconjugate distribution in the human fundic mucosa revealed by lectin- and glycoprotein-gold cytochemistry. | the glycoconjugates of the human fundic mucosa were characterized at the ultrastructural level by means of direct (helix pomatia agglutinin-gold complex) and indirect lectin techniques (concanavalin a and horseradish peroxidase-gold complex; wheat germ agglutinin and ovomucoid-gold complex). surface mucous cells and mucous neck cells secreted o-glycoproteins with n-acetylgalactosamine and n-acetylglucosamine residues at the non reducing terminus of the saccharidic chain. the secretory granules o ... | 1990 | 2081692 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| use of peldri ii as a sublimation dehydrant in place of critical-point drying in fracture-label cytochemistry and in backscattered electron imaging fracture-label. | peldri ii, a fluorocarbon compound solid at room temperature, was used as a sublimation dehydrant in place of critical-point drying (cpd) in freeze-fracture cytochemistry. its applicability to the fracture-label replica method was demonstrated using freeze-fractured rat pancreas labeled with helix pomatia lectin-gold complex and phospholipase a2-gold complex for the detection of glycoconjugates and phospholipids, respectively. no difference in morphology and labeling pattern was detected in cpd- ... | 1990 | 2153786 |
| interaction of bluetongue virus with bovine lymphocytes. | freshly isolated, and established, cultures of bovine peripheral blood mononuclear leukocytes (pbmls) were exposed to bluetongue virus (btv) for the purpose of defining potential lymphotropism. pbml cultures were established in the presence of interleukin 2 (il-2) and mitogen and maintained either as bulk culture or were cloned prior to infectivity studies. all cultures appeared to be of the t cell phenotype based on the following characteristics: binding of t lymphocyte-specific lectins (i.e. p ... | 1990 | 2155289 |
| studies on the hatching of miracidia of dicrocoelium dendriticum (author's transl). | the eggs of dicrocoelium dendriticum were induced to open by solutions of formic acid and caproic acid (table 1). the miracidia hatched in o2-free water after the eggs had been dried with n2 or in vacuum. the miracidia were able to live for 3 hours if water contained 20 mm nacl, 10 mm kcl, and 1 mm cacl2. ca++-ions are obviously necessary for the mobility of miracidia. the experimental use of intestinal juice of the roman snail helix pomatia gave hatching results which were dependent on the abse ... | 1975 | 3901 |
| characterization of the high mr glycoprotein (gp300) of equine herpesvirus type 1 as a novel glycoprotein with extensive o-linked carbohydrate. | the high mr glycoprotein (gp300) of equine herpesvirus type 1 was found to have an mr, estimated by sds-page, of over 400,000 and was confirmed as being a surface glycoprotein by 125i-labelling. in contrast to [3h]glucosamine, gp300 showed very low levels of [3h]glucosamine, gp300 showed very low levels of [3h]mannose incorporation. the mr of gp300 showed no detectable change upon treatment of purified virus with n-glycanase, and showed only a small change in virus-infected cells treated with tu ... | 1990 | 2172454 |
| time courses of lidocaine effects on sodium membrane currents in small and large neurons. | time courses of effects of lidocaine on sodium currents and sodium dependent action potentials were studied in somata of small and large neurons. cultured rat sensory spinal ganglion cells (diameter: 30 microns) and neurons of the buccal ganglion of helix pomatia (diameter: 150 microns) served as the test cells. the latency of the suppressive action of lidocaine was the longer the larger the of the cells was. maximal blocking effects occurred within 10 min in sensory spinal ganglion cells and wi ... | 1990 | 2177020 |
| effects of alcohol on lectin binding affinity in rat gastric mucosa. | fluoresceinated lectins were employed to qualitatively evaluate cell surface carbohydrates, with and without ethanol exposure, in rat stomach mucosae. rats received 1 ml of saline, or 50% or 100% ethanol orally. after 30 min, tissue samples of the glandular stomach were retrieved, cryosectioned, and incubated with one of a panel of lectins. another set of sections was preincubated with neuraminidase to remove sialic acid residues. qualitative evaluation of lectin binding showed that although sev ... | 1990 | 2194768 |