| the disulfide bridges of the trypsin-kallikrein inhibitor k from snails (helix pomatia). thermal inactivation and proteolysis by thermolysin (author's transl). | isoinhibitor k is the main component of the complex mixture of isoinhibitors of broad specificity secreted into the mucus by the roman snail (helix pomatia). the disulfide pairing was determined after the amino acid sequence had been elucidated. two cystine-containing peptides with the disulfide bridges cys32-cys53 and cys32-cys53 plus cys7-cys57 were obtained after thermolytic hydrolysis of the native inhibitor at 80 degrees c and chromatographic separation of the peptides using se-sephadex. th ... | 1976 | 3462 |
| studies on the hatching of miracidia of dicrocoelium dendriticum (author's transl). | the eggs of dicrocoelium dendriticum were induced to open by solutions of formic acid and caproic acid (table 1). the miracidia hatched in o2-free water after the eggs had been dried with n2 or in vacuum. the miracidia were able to live for 3 hours if water contained 20 mm nacl, 10 mm kcl, and 1 mm cacl2. ca++-ions are obviously necessary for the mobility of miracidia. the experimental use of intestinal juice of the roman snail helix pomatia gave hatching results which were dependent on the abse ... | 1975 | 3901 |
| tyrosine hydroxylase in snail (helix pomatia) nervous tissue. | | 1976 | 5096 |
| improved hydrolysis of urinary 17-hydroxycorticosteroid glucuronides with beta-glucuronidase from helix pomatia, on adding sodium sulfate. | sodium sulfate increases the hydrolysis of urinary 17-hydroxycorticosteroid glucuronides with beta-glucuronidase preparations derived from helix pomatia because it removes the inhibitory activity of urinary high-molecular-weight substances. for maximum hydrolysis of urinary 17-hydroxycorticosteroid glucuronides, the hydrolysis 5 ml of urine, 0.5 ml of 2 mol/liter acetate buffer (ph 5.0) should be conducted in the presence of sodium sulfate (final concentration: 80 g/liter) with (a) 600 fishman u ... | 1977 | 21763 |
| [immunologic properties of various cell fractions of a wild strain and a mutant strain of saccharomyces cerevisiae hansen]. | some fractions of the saccharomyces cerevisiae cell wall have been prepared by the action of helix pomatia juice on intact cells. immunosera were obtained by injecting rabbits with these fractions. immunofluorescence reactions, obtained with these sera, show that some fractions of mannopeptides when extracted from a "smooth-colony" mutant strain, have lost antigenic determinants. | 1977 | 70263 |
| class-specific antibody titres (elisa) against the primary immunogen helix pomatia haemocyanin (hph) in man. | using the indirect elisa technique, igm, igg and iga antibody titres against the primary test immunogen helix pomatia haemocyanin (hph) could be measured. all twenty-seven normal volunteers (age range 26-74 years) developed maximal or high igm, igg and iga antibody titres 2 weeks after primary immunization with 1.0 mg hph subcutaneously. lower dose immunization resulted in submaximal responses. titre kinetics in the three ig classes correlated in height and time per person. there was no signific ... | 1978 | 80296 |
| [electron microscopic detection of blood group antigen a on human erythrocytes by means of ferritin- and gold-labelled protectin of helix pomatia]. | by means of ferritin- and gold-labelled protectin from the albumen gland of the edible snail helix pomatia the blood group antigen a was located on human erythrocytes of groups a1, a2 and a1b. with erythrocytes of groups o and b the reaction is negative. the antigen is focally distributed on the outer surface of the cell membrane. cells from groups a1 and a1b have an antigen a concentration about 4 times greater than a2 cells. the numbers of particles after tagging with ferritin or gold are comp ... | 1976 | 134531 |
| universality of gonadotropic hormone effect. effect of mammalian fsh on the ovotestis of the snail helix pomatia. | gonadotropic hormone (fsh) stimulated markedly spermatogenesis in the ovotestis of snails. this is indicative of the presence of receptors sensitive to gonadotropic hormone in snail spermatogonia. fsh had no influence on oogenesis. | 1975 | 168707 |
| hydrolysis of steroid glucuronides with beta-glucuronidase preparations from bovine liver, helix pomatia, and e. coli. | we determined the enzymic activity of beta-glucuronidase preparations from bovine liver, helix pomatia, and escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. we also studied the effect of na2so4 on the enzymic hydrolysis of several substrates with the three preparations of beta-glucuronidase. na2so4 increases the rate of hydrolysis of all substrates with beta-glucuronidase from bovine liver. hydrolysis of a steroid glucoronide with beta-glucuronidase from hel ... | 1977 | 189953 |
| adherent cells as targets in mitogenic t-lymphocyte stimulation: replacement of lymphocyte-bound mitogens by nonmitogenic substances. | the sequence of events in mitogenic t-lymphocyte activation was investigated by employing purified mouse peritoneal adherent cells and t-lymphocyte populations. after treatment of adherent cells with the oxidizing agents sodium periodate or neuroaminidase plus galactose oxidase and removal of the mitogens, the cells acquired the ability to stimulate purified t-lymphocytes added subsequently. for this process of t-lymphocyte activation, the additional presence of the membrane interacting substanc ... | 1979 | 226472 |
| neuraminidase inhibition by chemically sulphated glycopeptides. | chemically sulphated glycopeptides (derived from pig duodenal mucosa) inhibited clostridium perfringens neuraminidase (ec 3.2.1.18) activity in a ph-dependent manner. analysis of inhibition kinetics data indicated that, although the enzyme inhibition could not be categorized into any of the classical types of inhibition, it could be interpreted as a function of the size and shape of the substrates used. the enzyme activity was inhibited by 86% and 40% when tested with bovine submaxillary-gland m ... | 1979 | 227363 |
| quantitative precipitin studies on the specificity of an extract from tridacna maxima (röding). | haemolymph from the clam tridacna maxima precipitated with purified h-blood-group substances, helix pomatia galactogen, and pneumococcus type xiv polysaccharide. although gel diffusion, gel electrophoresis, and inhibition experiments indicated that only a single precipitating lectin was present in the haemolymph, quantitative precipitin and haemagglutination results suggested that a second agglutinin with anti-h-like specificity was also present. evidence obtained from hapten inhibition experime ... | 1975 | 236090 |
| [gelelectrofocusing of protektin anti ahp from the albumin gland of helix pomatia (author's transl)]. | the protektin anti ahp from the albumine gland of helix pomatia is a very interesting substance because of its ability to agglutinate erythrocytes of blood group a as well as group c streptococci. purification is possible by reversible absorption on sephadex g200. isoelectric focusing in a sucrose density gradient is difficult, because the protein precipitates at the isoelectric point. the agglutinin, which is homogeneous in disk electrophoresis experiments (in an alkaline gel system as well as ... | 1975 | 242131 |
| helix pomatia a hemagglutinin: selectivity of binding to lymphocyte surface glycoproteins on t cells and certain b cells. | human and mouse lymphocytes were surface-labeled by lactoperoxidase-catalyzed iodination, or by galactose oxidase oxidation followed by reduction with tritiated sodium borohydride. the labeled cells were lysed with nonidet p-40. proteins binding to helix pomatia a hemagglutinin (hp) were isolated by affinity chromatography on hp-sepharose and analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography. a major cell surface glycoprotein (apparent mol. wt. 150 000, us ... | 1978 | 309819 |
| a new surface marker on mouse natural killer cells: receptors for helix pomatia a hemagglutinin. | natural killer (nk) cells in normal mouse spleen have so far been found to lack conventional cell surface markers. recently, neuraminidase treatment has been shown to uncover receptors for the a hemagglutinin of the snail helix pomatia (hp) on populations (preferentially t cells) of human blood lymphocytes. we have used hp coupled to sepharose in columns to fractionate mouse spleen cells. fractions consisted of passed cells (fraction i) and cells eluted with the competitive hapten n-acetyl-d-gal ... | 1978 | 309820 |
| a new rat lymphocyte surface marker: characterization and separation of cells with receptors for helix pomatia hemagglutinin. | thirty-two percent of neuraminidase-treated da rat spleen lymphocytes and 48% of lymph node lymphocytes possess receptors for helix pomatia hemagglutinin (hp). moreover, these hp-receptor-bearing cells can be separated from b cells by affinity chromatography on hp-sepharose columns. the virtual absence of immunoglobulin (ig) receptors and the close correlation with reported t-cell content of these lymphoid tissues suggest that hp-receptor lymphocytes are probably t cells and that hp may provide ... | 1977 | 322264 |
| receptors for helix pomatia a haemagglutinin (hp) on a subpopulation of human b cells. | receptors for helix pomatia a haemagglutinin (hp) have earlier been found on neuramidinase treated t-lymphocytes in human peripheral blood. in contrast, the majority of the b-lymphocytes, characterized by surface bound igm and/or igd(sig)lack these receptors. double marker experiments with fluorochrome labelled reagents have now shown that a minor fraction (3--24%) of the igm/d bearing lymphocytes in normal human blood also have hp-receptors. these hp+ b-cells constitute approximately 1% of the ... | 1978 | 349677 |
| enzyme immunoassay for total oestrogens in pregnancy plasma or serum. | we have developed an enzyme immunoassay for total oestrogens in pregnancy serum of plasma, using horseradish peroxidase (hrp) as the marker enzyme. a test combination consisting of an antiserum against oestriol-16/17-monosuccinyl-albumin and oestriol-16/17-monosuccinyl-hrp yielded a sensitive system, which reacted to approximately the same extent with oestrone, oestradiol, oestriol and their 16- and 17-conjugates. samples had to be diluted 1 to 10 to avoid interference of plasma factors with the ... | 1978 | 361293 |
| characterization of human lymphocytes bearing fc receptors for ige isolated from blood and lymphoid organs. | human lymphocytes isolated from adult peripheral blood and cord blood, tonsils, adenoids and spleens were analysed for fc receptors for ige (fcepsilon) by rosette assays. the fcepsilon+ cells were also characterized for their class of surface immunoglobulin (sig), complement receptors, receptors for sheep erythrocytes (e), and helix pomatia a haemagglutinin (hp), and their abilities to phagocytoze and adhere. the average number of fcepsilon+ cells was in adult peripheral blood 1.2 +/- 0.4%, in c ... | 1979 | 368962 |
| purification of some glycoside hydrolases by affinity chromatography. | two glycoproteins have been isolated from the cell walls of baker's yeast. one is a glucan-protein complex which has been partially characterised as having a branched carbohydrate structure composed of chains of (1 leads to 3)-linked beta-d-glucosyl residues, some of which are attached by (1 leads to 6)-linkages to the main chain. immobilization of this glycoprotein was achieved by covalent attachment to sepharose, and the product was used to isolate a number of (1 leads to 3)-beta-d-glucan hydr ... | 1977 | 409497 |
| the isolectins of helix pomatia. separation by isoelectric focusing and preliminary characterization. | helix pomatia lectin was fractionated into several (at least 12) components by isolectric focusing in pharmalytetm. eight of these were recovered from a preparative column experiment and shown to be essentially pure by analytical electrofocusing in polyacrylamide gel slabs. the lectin components had haemagglutinating activity against human group a erythrocytes and similar molecular weights of 80 000 as determined vidual components showed slight variations in composition. the components are consi ... | 1979 | 465535 |
| heterogeneity and mechanism of action of human natural killer lymphocytes: differential distribution of receptors for helix pomatia haemagglutinin (hp receptors). | neuraminidase-treated lymphocytes from the peripheral blood of normal human donors were fractionated on columns charged with helix pomatia haemagglutinin (hp) coupled to sepharose 4b. while lymphocytes lacking hp receptors (hp-) passed directly through the column (fraction i), lymphocytes with hp receptors (hp+) were subsequently eluted in two distinct fractions with two different concentrations of the competitive hapten n-acetyl-d-galactosamine (fraction ii and iii, respectively). the natural c ... | 1979 | 493880 |
| synaptic and hormonal modulation of a neuronal oscillator: a search for molecular mechanisms. | 1. the central ganglia of a number of gastropod molluscs (including the marine snail aplysia californica and the terrestrial snail helix pomatia) contain neurones which exhibit endogenous patterns of oscillatory activity. 2. this oscillatory activity can be modulated for long periods of time by synaptic and hormonal stimulation. 3. stimulation of appropriate pre-synaptic nerves causes long-lasting hyperpolarization in these neurones, with complete abolition of oscillatory activity. this synaptic ... | 1979 | 512575 |
| [preparation and properties of protoplasts from saccharomyces vini k-42]. | lytic enzymes from the edible snail--helix pomatia--were used to obtain protoplasts of yeast saccharomyces vini k-42. the yeast grown in aerobic conditions proved more sensitive. the resultant protoplasts oxidized glucose and sucrose and did not oxidize maltose. | 1979 | 515001 |
| [lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)]. | lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ... | 1979 | 547035 |
| carbohydrate binding specificity of four n-acetyl-d-galactosamine- "specific" lectins: helix pomatia a hemagglutinin, soy bean agglutinin, lima bean lectin, and dolichos biflorus lectin. | | 1977 | 560855 |
| studies on lectins. xxxvi. properties of some lectins prepared by affinity chromatography on o-glycosyl polyacrylamide gels. | a number of lectins has been purified by affinity chromatography on o-glycosyl polyacrylamide gels. the lectins isolated (and the particular sugar ligands used in the affinity carriers) are as follows: anguilla anguilla, serum (alpha-l-fucosyl-), vicia cracca, seeds; phaseolus lunatus, seeds; glycine soja, seeds; dolichos biflorus, seeds; maclura pomifera, seeds; sarothamnus scoparius, seeds; helix pomatia, ablumin glands; clitocybe nebularis, fruiting bodies (all n-acetyl-alpha-d-galactosaminyl ... | 1978 | 563738 |
| [fractional protein makeup of candida utilis yeast protoplasts in the process of their growth and development]. | candida utilis ibfmy-405 was grown in a synthetic medium with glucose. cells taken at the logarithmic phase of growth were studied. the cells were treated with the enzyme from helix pomatia to prepare protoplasts which were separated by differential centrifugation into groups according to their size. three protein fractions were isolated from each group and the amino acid composition of the proteins was determined. proteins of the first fraction (cytoplasmic) prevailed in all of the protoplast g ... | 1979 | 571510 |
| a versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations. | a procedure for cell fractionation using lectin-affinity chromatography is described. it consists of a single affinity adsorbent, hog gastric mucin blood group a+h substance covalently coupled to sephadex or sepharose, to which lectins of various specificities can bind. the complex formed, lectin in equilibrium hog a+h substance-sephadex, then serves as an affinity probe for isolating and fractionating cells. the lectins from ulex europaeus, lotus tetragonolobus, helix pomatia, dolichos biflorus ... | 1979 | 573269 |
| immunochemical detection of the thomsen-friedenreich antigen (t-antigen) on platelet plasma membranes. | platelet plasma membranes were found to possess the disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosamine which was measured by gas chromatography after release by alkaline borohyride treatment and desialylation. immunological evidence using the specific lectins from arachis hypogoea and agaricus bisporus and an anti-t serum confirmed the presence of this disaccharide, the immunodominant group of the thomsen-friedenreich antigen (t-antigen). this receptor was only found after prior neura ... | 1978 | 580492 |
| electron microscopic detection of the cryptantigen ahumhp (friedenreich antigen) on human erythrocytes by means of gold-labelled agglutinin from helix pomatia. | using the gold-labelled agglutinin anti-ahp from helix pomatia (edible snail) the ultrastructural site and distribution of the cryptantigen (friedenreich antigen) ahumhp was revealed on neuraminidase treated human o and b erythrocytes. on a1 and a2 cells the antigen was demonstrated simultaneously with the blood group antigen a by labelling the cells with anti-ahp-ferritin followed by removing the neuraminic acid and labelling with anti-ahp-gold. the cryptantigen is focally distributed on the ce ... | 1977 | 602347 |
| opsonizing effect of serum and albumin gland extracts on the elimination of human erythrocytes from the circulation of helix pomatia. | | 1978 | 632614 |
| substrates for the assay of alpha-l-iduronidase. | procedures are described for the preparation of two disaccharides, 4-o-alpha-l-iduronosyl-2,5-anhydro[3h]mannitol and 3-o-alpha-l-iduronosyl-2,5-anhydro[3h]-talitol, from heparin and dermatan sulfate, respectively. these disaccharides lend themselves to an easy assay of alpha-l-iduronidase which is based on the fractionation of the liberated neutral anhydro[3h]mannitol or anhydro[3h]talitol from the unreacted substrate by adsorption of the latter to dowex 1. investigation of the reaction conditi ... | 1978 | 709885 |
| a 1h nuclear-magnetic-resonance study of the solution conformation of the isoinhibitor k from helix pomatia. | the isoinhibitor k from helix pomatia was investigated by high-resolution 1h nuclear magnetic resonance at 360 mhz. detailed studies of the labile protons and the resonances of the aromatic residues indicated extensive homologies between the spatial structures of the snail inhibitor, the basic pancreatic trypsin inhibitor (kunitz) from bovine tissue and the cos colostrum trypsin inhibitor. comparison of these three homologous inhibitors indicated that the overall flexibility of the globular prot ... | 1978 | 710398 |
| a sulphate metabolite of trimethoprim in goats and pigs. | 1. urine from goats and pigs after intravenous administration of [14c]trimethoprim contained an unidentified fraction of 'other water-soluble metabolites'. this fraction accounts for 56% of the administered dose in goat urine and 65% in pig urine. 2. a sulphate metabolite comprising the major part of the latter fraction in both goat and pig urine was isolated by gel filtration and paper chromatography and identified by spectroscopic methods as 2,4-diamino-5-(3,4-dimethoxy-5-sulphooxybenzyl)pyrim ... | 1978 | 726512 |
| interaction of peanut agglutinin with human lymphocytes. binding properties and topology of the receptor site. | the relationship between the mitogenic lectin pna and other mitogenic and non-mitogenic lectins was investigated. pna labelled with 125i was found to bind equally well to t and b lymphocytes, after neuraminidase treatment, with 3.88 times 10(6) and 3.73 times 10(6) binding sites respectively. only the t cell fraction was stimulated, however, and only after neuraminidase treatment. preincubation of neuraminidase-treated cells with non-mitogenic lectins and antiserum which appeared to bind to the ... | 1978 | 726549 |
| enrichment of helix pomatia (hp) lectin binding variant from the ta3st mouse ascites tumor by repeated column selection. | | 1978 | 738331 |
| immuno-chemical studies on the alkali-labile carbohydrate chains of human serum glycoproteins. | human serum glycoproteins can be classified into those containing n-acetyl-d-galactosamine and into those lacking this hexosamine. the n-acetyl-d-galactosamine-containing serum glycoproteins have alkali-labile chains containing this hexosamine linked o-glycosidically to hydroxy amino acids. these alkali-labile chains can be demonstrated in neuraminic acid free serum glycoproteins by gas liquid chromatography and by using precipitating lectins from invertebrates and plants. they are represented b ... | 1979 | 762504 |
| [decomposition of steroids during incubation with beta-glucuronidase and during storage of urine]. | androsterone, etiocholanolone, pregnanetriol, dehydroepiandrosterone, pregnanediol, tetrahydrocortisol, 5-pregnenolone and 11-beta-oh-androsterone were incubated with beta-glucuronidase preparations (helix pomatia, bovine liver and e. coli) for 96 hrs at 37 degrees c. after extraction and silylation they were gas-chromatographed. the first 3 steroids were left practically intact. the least decomposition of the last 5 steroids occurred with the liver enzyme. testosterone and 11-ketoandrosterone w ... | 1975 | 765125 |
| the interaction of nonmitogenic and mitogenic lectins with t lymphocytes: association of cellular receptor sites. | the relationship between the surface receptors on neuraminidase-treated human blood lymphocytes for the mitogenic lectins phaseolus vulgaris leukoagglutinin (la), concanavalin a (con a) and soy bean agglutinin (sba) and the non-mitogenic lectin helix pomatia a hemagglutinin (hp) was investigated. two different techniques, co-capping with different fluorochrome-labeled lectins and cell binding-inhibition experiments with 125i-labeled lectins, were used. the results demonstrated that the nonmitoge ... | 1976 | 772792 |
| an ultrastructural analysis of protoplast-spheroplast induction in cryptococcus neoformans. | protoplasts-spheroplasts of a human isolate of cryptococcus neoformans were prepared using the gut enzyme of helix pomatia. the induction process, as studied by transmission electron microscopy, occurred in two stages. early in the induction process, protoplasts-spheroplasts emerged from whole cells through a break in the cell wall-capsule envelope. later, a gradual dissolution of the entire cell wall occurred releasing the intact protoplast-spheroplast. a comparison of protoplasts-spheroplasts ... | 1976 | 788868 |
| occurrence of a blood group a-like substance in eggs of the prosobranch snail pomacea canaliculata. | in the eggs of the prosobranch snails pomacea canaliculata and pomacea insularum a blood group a-like substance has been detected by anti-a from the snails helix pomatia, helix aspersa and cepaea nemoralis. | 1976 | 815102 |
| [use of an agglutinin from the albumin gland of the edible snail for immunoelectron microscopic localization of cell membrane antigens with terminal n-acetyl-d-galactosamine]. | | 1975 | 829939 |
| radioimmunological determination of urinary tetrahydroaldosterone. | a method is presented for radioimmunological determination of 3alpha, 5beta-tetrahydroaldosterone. it is based upon the reactivity of this steroid with an antiserum induced by the 3-carboxymethyloxime of 18, 21-aldosterone diacetate conjugated with bovine serum albumin. one hundred microliters of urine enzymatically hydrolyzed with an helix pomatia preparation, containing tritiated tetrahydroaldosterone for the yield calculation, were extracted with dichloromethane and chromatographed on a small ... | 1977 | 910248 |
| high-performance liquid chromatographic separation and photometric detection of phospholipids. | a rapid and efficient method for the separation of (phospho)lipids by high-performance liquid chromatography using n-hexane-2-propanol-water mixtures as the solvent system is described. the lipid separation occurs on silica gel columns and the individual components are monitored directly by uv absorption at 206 nm. of a total lipid extract from erythrocytes as well as suboesophageal ganglia of the snail helix pomatia, a complete separation is achieved of cholesterol, phosphatidic acid, phosphati ... | 1977 | 914946 |
| alkali-labile oligosaccharides from glycoproteins of different erythrocyte and milk fat globule membranes. | phenol extraction of horse, sheep, cow, pig and human erythrocyte membranes and human milk fat globule membranes gave glycoprotein fractions, all of which were shown by gas chromatography to contain the reduced disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosaminital after treatment with alkaline borohydride. cow and pig erythrocyte membrane glycoproteins were found however to contain much lower amounts than the erythrocyte membrane glycoproteins of the other species tested. after gel fi ... | 1976 | 963062 |
| determination of arginine in the reactive site of proteinase inhibitors by selective and reversible derivatization of the arginine side chain. | reversible and selective modification of the guanidino group by 1,2-cyclohexanedione or 2,3-butanedione in borate buffer was applied to identify arginine residues in the reactive sites of proteinase inhibitors. the ability of the method to distinguish between arginine and lysine residues was examined. trypsin inhibitors with arginine at the reactive site were almost completely inactivated within 2 h, e.g. the low-molecular weight inhibitors from soybeans (kunitz), peanuts and porcine seminal pla ... | 1976 | 964925 |
| analysis by a plaque assay of igg- or igm- dependent cytolytic lymphocytes in human blood. | when monolayers of bovine erythrocytes (eb) were exposed to purified human blood lymphocytes and either igg or igm fractions of rabbit anti-eb serum, clear zones (plaques) appeared when eb had been lysed by antibody-dependent effector cells (k cells). igg-dependent plaque formation was complete by 20 h of incubation, while the igm-dependent reaction required 40 h. the estimated minimal numbers of plaque forming cells (pfc) were 5.6% (igg) and 2.0% (igm) of the added lymphocytes. inhibition exper ... | 1976 | 993729 |
| trypsin-kallikrein isoinhibitor k (type kunitz) from snails (helix pomatia). purification and characterization. | a basic proteinase inhibitor, isoinhibitor k, was purified by se-sephadex c-25 column chromatography from the mixture of acid-stable and heat-stable isoinhibitors of the snail (helix pomatia). isoinhibitor k is homogeneous in polyacrylamide gel, cellulose acetate and polyacrylamide-dodecylsulfate electrophoresis. from the electrophoretic mobility in dodecylsulfate-polyacrylamide gel and apparent molecular weight of 6500 +/- 200 was estimated. from the amino acid composition the inhibitor consist ... | 1975 | 1081050 |
| receptors for helix pomatia a haemagglutinin on leukaemic lymphocytes from patients with chronic lymphocytic leukaemia (cll). | blood lymphocytes from thirteen patients with cll were studied for surface-bound ig (sig), fc receptors (ea rosettes), receptors for sheep erythrocytes (e rosettes) and receptors for helix pomatia a haemagglutinin (hp), a carbohydrate-binding protein with specificity for n-acetyl-d-galactosamine and related sugars. fluorescein-labelled hp binds to subpopulations of human peripheral blood lymphocytes (pbl) treated with neuraminidase. in normal peripheral blood, hp binds to the t-lymphocytes while ... | 1976 | 1086751 |
| fractionation of human blood lymphocytes on helix pomatia a haemagglutinin coupled to sepharose beads. | treatment of human blood lymphocytes with neuraminidase has previously been shown to uncover receptors for the a haemagglutinin of the snail helix pomatia (hp). neuraminidase-treated lymphocytes were now fractionated on columns charged with large sepharose particles to which hp had been coupled covalently. hp-receptor negative (hp-) lymphocytes passed the columns while hp-receptor positive (hp+) lymphocytes were retained. the latter cells were eluted by addition of the competitive hapten n-acety ... | 1976 | 1088709 |
| concanavalin a and other lectins in the study of tumor cell surface organization. | cell surface structures of two mouse ascites tumors were studied using lectins. the tumors are sublines of the spontaneous mammary adenocarcinoma ta3 in strain a, differing in two main characteristics. subline ta3-st grows only in syngeneic mice and has high expression of h-2 antigens. subline ta3-ha, in contrast, proliferates in all mouse strains, and has low amounts of exposed h-2 antigens. concanavalin a (con a), phytohemagglutinin (pha) and helix pomatia anti a hemagglutinin (hp) were used i ... | 1975 | 1098413 |
| [immunoelectronmicroscopic localization of cell wall antigens in streptococci. ii. localization of the group-specific polysaccharide of group c streptococci with ferritin- and peroxidase-labelled helix pomatia-agglutinin (author's transl)]. | the carbohydrate of the cell wall of group c streptococci is one of the best known group-specific streptococcal antigens with respect to its chemical structure. in the present paper, the ultrastructural location of the carbohydrate was investigated by means of immunoelectronmicroscopic techniques. besides group-specific antibodies, the specific binding of an agglutinin (protectin anti-ahp) of the edible snail (helix pomatia) to structures with terminal n-acetylgalactosamine was used to demonstra ... | 1975 | 1154918 |
| [production of candida lipolytica protoplasts]. | optimal conditions were found for the production and isolation of the protoplasts of candida lipolytica. the maximum amount of the protoplasts was produced after 90 minutes of the incubation with a crude preparation of the enzyme from helix pomatia (100 mg/g wet biomass). longer incubation results in lysis of the protoplasts and structural damages of the intracellular components. the yield of the protoplasts does not depend on the nature of stabilizing agent. a decrease in the stabilizer concent ... | 1975 | 1160627 |
| [spheroplasts of torulopsis candida yeasts and their oxidizing activity]. | the paper describes the conditions in which the spheroplasts of the yeast torulopsis candida ibfm-y-127 with a high respiration rate can be isolated. the preliminary incubation of the cells with sh-reagents has to be carried out in a buffer without an osmotic stabilizer, and the incubation in a medium containing 0.6 m kcl, 0.1 m mgso4, 0.1 mkh2po4, ph 5.2. in these conditions the cells are incubated with the enzyme from helix pomatia during 15 to 20 minutes, and the yield of the spheroplasts is ... | 1975 | 1172174 |
| further characterization of some heterophile agglutinins reacting with alkali-labile carbohydrate chains of human erythrocyte glycoproteins. | the nature of the receptor sites for several agglutinins is characterized by hemagglutination inhibition assays. the inhibitory activity of human erythrocytes glycoproteins, from which sialic acid, sialic acid and galactose or alkali-labile oligosaccharides have been removed, is compared to the inhibitory effect of compounds with known structure. it is shown that the lectin from arachis hypogea and anti-t bind to alkali-labile galactosyl-residues. agglutinins from bauhinia purpurea and variegata ... | 1975 | 1172890 |
| the amino-acid sequence of isoinhibitor k form snails (helix pomatia). a sequence determination by automated edman degradation and mass-spectral identification of the phenylthiohydantoins. | performic-acid-oxidized isoinhibitor k of snails (helix pomatia) was subjected to arginine-directed tryptic proteolysis. six peptide fragments including one overlap peptide from limited cleavage of the arg-3-pro-4 bond were purified to homogeneity. four arginine peptides and the c-terminal peptide were sequenced by automatic edman degradation using a special peptide program. the phenylthiohydantoins were all identified by chemical ionization mass spectrometry, except four cysteic acid residues t ... | 1975 | 1183446 |
| [the enzymatic hydrolysis of cell wall applied to yeasts classification (author's transl)]. | the rigid structure of yeast cell wall may be a taxonomic criterion. using the helix pomatia juice (with mercaptoethylamine hydrochloride) as a polyenzymatic system, we have always obtained protoplasts from ascomycetous yeasts and never from basidiomycetous yeasts tested. then, we have applied this enzymatic analysis to the fungi imperfecti yeasts and, according to this test, separated genus looking like basidiomycetous behaviour: trichosporon, pityrosporum, rhodotorula, cryptococcus and non fer ... | 1975 | 1190637 |
| the lack of mitogenic response of neuraminidase-treated and untreated human blood lymphocytes to divalent, hexavalent, or insoluble helix pomatia a hemagglutinin. | | 1975 | 1193182 |
| [neuraminidase induced hemolytic anemia. experimental and clinical observations (author's transl)]. | recently, increasing attention has been focussed on the in vivo action of neuraminidase as possible pathogenetical factor of hemolytic anemia and even hemolytic-uremic syndrome. neuraminidase action in red cell membranes results in the release of neuraminic acid, and thereby the uncovering of previously hidden receptors, socalled cryptantigens. with special reference to the phythemagglutinin anti-tah from the peanut (arachis hypogae) and the agglutinin anti-ahp from the albumin gland of the smal ... | 1976 | 1246237 |
| immunochemical detection of the thomsen-friedenreich antigen (t-antigen) on the pig lymphocyte plasma membrane. | a method for the isolation of lymphocytes from pig peripheral blood and preparation of plasma membranes was developed. the method resulted in a ten-fold increase in 5'-nucleotidase activity and neuraminic acid, relative to protein. a cholesterol/phospholipid ratio of 0.93 was obtained. alkaline borohydride treatment of isolated plasma membrane, after desialylation, released the disaccharide beta-d-galactosyl-(1 leads to 3)-n-acetyl-d-galactosaminitol (the immunodominant group of the t-antigen), ... | 1976 | 1278165 |
| modulation of voltage-activated ion currents on identified neurons of helix pomatia l. by interleukin-1. | 1. the effect of interleukin-1 (il-1) was studied on voltage-activated ion currents of the identified central neurons of helix pomatia l. using a two-microelectrode voltage clamp. the voltage-activated inward current (ica) was decreased, whereas the outward current (i(net) k) was increased by il-1. 2. il-1 affects both the transient and the delayed rectifying potassium currents. the il-1 modulatory effect on the voltage-activated ion currents was voltage and dose dependent. the threshold concent ... | 1992 | 1281756 |
| comparative study of prostatic carcinoma bone metastasis among japanese in japan and japanese americans and whites in hawaii. | metastatic prostate carcinomas in autopsy cases from three populations 49 cases of indigenous japanese, 29 cases of japanese americans and 14 from whites in hawaii) were compared in terms of their clinicopathological, immunohistochemical (tenascin and ras p21) and lectin binding (helix pomatia antigen, hpa) properties. only the clinicopathological features were analyzed in the cases of whites in hawaii. the results indicate that poorly differentiated carcinoma is less common, whereas distant met ... | 1992 | 1283503 |
| early increase in lymphocyte cyclic nucleotide phosphodiesterase activity upon mitogenic activation of human peripheral blood mononuclear cells. | cyclic nucleotide phosphodiesterase activity of human peripheral blood mononuclear cells was significantly increased following a short (30 min) incubation with the mitogenic lectin concanavalin a. con a stimulated phosphodiesterase activity to the same extent whatever the subcellular compartment (homogenate, cytosol or pellet). further separation of the con a-activated mononuclear cells into lymphocyte-enriched and monocyte-enriched populations showed that the con a-induced increase of phosphodi ... | 1992 | 1309823 |
| quantitative changes of ricinus communis agglutinin i and helix pomatia lectin binding sites in the acrosome of rat spermatozoa during epididymal transit. | during passage through the epididymis, spermatozoa undergo a number of changes which result in their acquisition of fertility and motility. some of the changes that occur include loss of the cytoplasmic droplet and changes in sperm morphology, metabolism and properties of the nucleus and plasma membrane. changes have also been reported in the acrosomic system of mammalian spermatozoa during their transit through the epididymis. in the present study, the quantitative changes of the glycoconjugate ... | 1992 | 1385371 |
| mannose-induced dysmorphogenesis of metanephric kidney. role of proteoglycans and adenosine triphosphate. | because various fetal anomalies are seen in diabetic offspring, we examined the effects of sugars on proteoglycans (pgs): extracellular matrix (ecm) macromolecules modulating morphogenesis. 13-d-old mouse metanephric kidney explants were exposed to mannose for 7 d and labeled with [35s]sulfate, [35s]-methionine, or [3h]thymidine. mannose exposure caused reduction in kidney size and disorganization of ureteric bud branches with inhibition of glomerulogenesis. tissue autoradiographic and immunoflu ... | 1992 | 1401058 |
| expression of glycoconjugates in the human fetal cochlea. | the distribution of glycoconjugates in the human fetal cochlea was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). the tectorial membrane (tm) in the 11- and 15-week-old human fetuses was labelled with wga, apa, rca120 and cona, but not with uea-i and hpa. in the 19-week-old fetuses, the reaction of the tm ... | 1992 | 1442009 |
| comparative lectin histochemistry on taste buds in foliate, circumvallate and fungiform papillae of the rabbit tongue. | taste buds (tb) in the foliate, circumvallate and fungiform papillae of the rabbit tongue were examined with lectin histochemistry by means of light (lm) and electron (em) microscopy. biotin- and gold-labeled lectins were used for the detection of carbohydrate residues in tb cells and subcutaneous salivary glands. at the lm level, the lectins of soybean (sba) and peanut (pna) react with material of the foliate and circumvallate taste pores only after pretreatment of the section with neuraminidas ... | 1992 | 1452451 |
| lectin binding to secretory structures, the cuticle and the surface coat of toxocara canis infective larvae. | toxocara canis infective larvae are known to produce abundant glycosylated molecules which may be found associated with the surface or secreted into their environment. using a range of fluorescein-conjugated and gold-conjugated lectins, the localization of particular carbohydrates was defined on the surface of live parasites, and internally at the ultrastructural level. surface exposure of n-acetyl galactosamine and n-acetyl glucosamine was deduced by binding of fitc-conjugated helix pomatia (hp ... | 1992 | 1454426 |
| impact of o-glycosylation on the function of human intestinal lactase-phlorizin hydrolase. characterization of glycoforms varying in enzyme activity and localization of o-glycoside addition. | lactase-phlorizin hydrolase (lph) is an integral intestinal brush border membrane glycoprotein responsible for the hydrolysis of lactose, the primary carbohydrate in mammalian milk. to assess the role of n- and o-glycosylation on the function of lph, lectin-binding experiments combined with enzymatic and chemical deglycosylation of purified lph molecules were performed. these investigations provided evidence for the existence of two forms of brush border lph, an n-glycosylated molecule (lphn) an ... | 1992 | 1460044 |
| [characterization of streptococcus uberis with special consideration of supposed pathogenicity factors]. | streptococcus uberis, as one of the principal causes of bovine streptococcal mastitis, has been characterized serologically and biochemically. serological grouping of s. uberis revealed polysaccharide antigens of groups e, g, p and u. the biochemical properties of s. uberis, determined with the strep-zym identification system, differed clearly from those of s. agalactiae and s. dysgalactiae. some cultures of s. uberis produced the enzymes hyaluronidase and neuraminidase. in addition s. uberis pa ... | 1992 | 1497584 |
| separation and identification of the 4-hydroxyantipyrine sulphoconjugate. | in a previous study we observed, during separation of total antipyrine metabolites by high-performance liquid chromatography and after enzymatic hydrolysis, an unidentified peak corresponding to an ionic compound with pyrazolinone features. in the present study, this compound was identified as the 4-hydroxyantipyrine sulphoconjugate, and its structure was definitively confirmed by gas chromatographic-mass spectrometric analysis and by the use of pure synthetic substance. we also demonstrated the ... | 1992 | 1500444 |
| the use of immobilized lectins in the separation of staphylococcus aureus, escherichia coli, listeria and salmonella spp. from pure cultures and foods. | lectins from helix pomatia, canavalia ensiformis, agaricus bisporus and triticum vulgaris agglutinated cultures of staphylococcus aureus, escherichia coli, listeria and salmonella spp. this agglutination was specific as it was inhibited (except with a. bisporus lectin) by the competing sugar substrates. the ability of three of these lectins, immobilized on a variety of supports, to separate these micro-organisms from pure cultures was investigated. immobilization of the lectins on magnetic micro ... | 1992 | 1512177 |
| [characteristics of obtaining protoplasts from 2 strains of tolypocladium inflatum subsp. blastosporum]. | the optimal conditions for preparing protoplasts with high yields by using the cells of two (low and high potent) isogenic cyclosporine-producing tolypocladium strains were developed. a specific medium containing 0.5 per cent yeast autolysate (by dry weight) and 3 per cent glucose was used. when grown on this medium the cells of the highly potent strain 847 acquired a yeast-like shape. high yields of protoplasts prepared from the low potent strain 43 mycelium were obtained via prior incubation w ... | 1992 | 1514857 |
| histochemical localization of glycoconjugates in the developing endolymphatic sac and vestibular end organs of the mouse. | the distribution of glycoconjugates in the endolymphatic sac (es) and vestibular end organs from the 12th gestational day (gd) in the developing mouse to the 6th day after birth was analyzed using six biotinylated lectins: wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 13th and 15th gd sections, the luminal contents in the fetal es were st ... | 1992 | 1518658 |
| agglutination of bordetella species by lectins. | phase i cells of bordetella pertussis but not those of b. parapertussis, b. bronchiseptica or b. avium were agglutinated by limulus polyphemus lectin. most strains of b. pertussis but not those of the other species were also agglutinated by helix pomatia lectin. in precipitation reactions between lectins and purified bordetella lipopolysaccharide (lps) preparations a similar pattern occurred. lectin agglutination provides a rapid presumptive method for the differentiation of b. pertussis from b. ... | 1992 | 1555759 |
| resistance of hamster bronchiolar epithelium to neutrophil elastase: investigation by cell surface lectin cytochemistry. | an intratracheal instillation of human neutrophil elastase (hne) causes accumulation of an excess number of secretory granules in the epithelial secretory cells lining the hamster bronchus. this chronic lesion, which we refer to as secretory cell metaplasia (scm), is not seen in the trachea or bronchioles. because luminal cell surface lectin binding is much higher in the trachea than in the bronchus, we concluded that tracheal resistance may be due to a protective glycoconjugate coat. in the pre ... | 1992 | 1572319 |
| intravenous thyrotropin (tsh)-releasing hormone releases human tsh that is structurally different from basal tsh. | to determine whether basal tsh differed structurally from trh-released tsh, the tsh obtained from 11 normal subjects before and after the iv administration of trh was characterized using lectin-affinity chromatography. tsh was applied to the following lectins: lentil, ricin (both before and after tsh treatment with neuraminidase), concanavalin-a, wheat germ, glycine max, helix pomatia, dolichos biflorus, arachis hypogaea, and vicia villosa (isolectin b4). after each column was washed to elute un ... | 1992 | 1592875 |
| subdomains of the rough endoplasmic reticulum in colon goblet cells of the rat: lectin-cytochemical characterization. | using lectin binding, we characterized subdomains of the rough endoplasmic reticulum (rer) in goblet cells of the rat colon. in this cell type, special rer regions can be differentiated on the basis of their content of low electron density and dilated cisternal spaces in conventional transmission electron microscopic preparations. the fine fibrillar content of these cisternal regions demonstrated high-affinity binding with lectins from wheat germ, helix pomatia, griffonia simplicifolia i-a4 and ... | 1992 | 1607641 |
| phosphofructokinase from mollusc muscle is activated by phosphorylation. | phosphofructokinase was purified from muscle tissue of two different molluscs, edible snails, helix pomatia (gastropoda), and mussels, mytilus edulis (bivalvia). under denaturing conditions, both enzymes had a molecular mass of 82 kda. in the presence of atp-mg2+, the enzymes were rapidly phosphorylated in vitro by the catalytic subunit of cyclic amp (camp)-dependent protein kinase purified from snail muscle and also by the c subunit of protein kinase from bovine heart. the extent of phosphoryla ... | 1991 | 1654810 |
| the prognostic value of tumor-associated carbohydrate structures correlated with gene amplifications in human breast carcinomas. | the effects of cell surface sugar chains combined with certain gene amplifications of breast cancers on the prognosis of patients were studied and the relationships between the sugar chains of cancer cells and amplifications of the proto-oncogenes c-myc, int-2 and c-erb b-2, evaluated. one hundred and fifty three human breast carcinoma tissues were investigated by an immunohistochemical technique using the avidinbiotin-peroxidase method with 1 lectin (hpa; helix pomatia) and 4 monoclonal antibod ... | 1991 | 1687600 |
| lectin binding patterns to terminal sugars of rat lung alveolar epithelial cells. | we used post-embedding cytochemical techniques to investigate the lectin binding profiles of rat lung alveolar epithelial cells. sections from rat lung embedded in the hydrophilic resin lowicryl k4m were incubated either directly with a lectin-gold complex or with an unlabeled lectin followed by a specific glycoprotein-gold complex. the binding patterns of the five lectins used could be divided into three categories according to their reactivity with alveolar epithelial cells: (a) the limax flav ... | 1990 | 1688898 |
| [determination of lectin receptors on the surface membranes of lymphoid cells]. | the authors describe a method for the detection of carbohydrate determinants contained by the cellular surface membrane glycosylated biopolymers, based on the use of horse radish peroxidase-conjugated arachis, helix pomatia, ricinus communis, lens, glycine max, sophora japonica lectins. the pattern of lectin receptor distribution on normal human blood lymphocytes, tonsillar lymphoid cells, and in some forms of leukemia and lymphoma is shown. the authors consider it essential that a lectin kit be ... | 1990 | 1692358 |
| a plasma membrane antigen of rat glomerular epithelial cells. antigenic determinants involving n-linked sugar residues in a 140-kilodalton sialoglycoprotein of the podocytes. | the aim of the present study was to investigate the antigenic make up of the plasma membrane of rat glomerular visceral epithelial cells (gep). a crude plasma membrane fraction (pm) was extracted by 1% sodium dioxycholate from isolated rat glomeruli. pm was digested with neuraminidase (nrd) and purified by the helix pomatia agglutinin (hpa)-affinity column. when studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the hpa-affinity purified plasma membrane fraction (hpa-pm) forme ... | 1990 | 1700199 |
| changes in glycoconjugates revealed by lectin staining in the developing airways of syrian golden hamsters. | lectin binding was studied in the developing airways of syrian golden hamsters on gestational days 11-16 (day 16 is the day of birth). the trachea and lungs were fixed in 4% formaldehyde-1% glutaraldehyde, 6% mercuric chloride-1% sodium acetate-0.1% glutaraldehyde, and 95% ethanol; embedded in paraffin; and stained with eight lectin-horseradish peroxidase conjugates: triticum vulgare (wga), dolichos biflorus (dba), helix pomatia (hpa), maclura pomifera (mpa), griffonia simplicifolia i-b4 (gsa i- ... | 1990 | 1700650 |
| cell type-specific lectin staining of the tracheobronchial epithelium of the rat: quantitative studies with griffonia simplicifolia i isolectin b4. | we compared lectin staining patterns to cell population densities, as determined by morphological criteria in rat airways. eight lectins were studied: griffonia simplicifolia i isolectin b4 (gsi-b4), arachis hypogaea (pna), wisteria floribunda (wfa), glycine maximus (sba), dolichos biflorus (dba), helix pomatia (hpa), ulex europaeus (uea-1), and maclura pomifera (mpa). two of the lectins strongly stained morphologically distinct cell subpopulations. gsi-b4 stained basal cells, and mpa stained no ... | 1991 | 1701188 |
| difference in the ability of blood group-specific lectins and monoclonal antibodies to recognize the abh antigens in human tissues. | twelve different kinds of blood group-specific lectins have been used along with monoclonal anti-a, -b and -h antibodies for detecting the corresponding antigens in selected human tissues. although most of the lectins recognized the antigens in the tissue sections examined, they displayed marked differences in their recognition patterns in certain tissues. helix asparsa agglutinin (haa), helix pomatia agglutinin (hpa) and monoclonal anti-a antibody recognized a antigens in the mucous cells of sa ... | 1990 | 1705925 |
| [fine structure studies and identification of lectin receptors on the viral envelope of two hiv-isolates]. | the electron microscopic particle findings were compared with the levels of revertase in corresponding samples over a longer period of time, and a good correlation was found. comparative investigations of the fine-structure of two hiv isolates did not reveal any morphological differences. it can be assumed, on the basis of the comparative studies on lectin receptors using helix pomatia lectin, that the viral envelopes of the two isolates are equipped similarly with n-acetyl-d-galactosamine. the ... | 1990 | 1706878 |
| experimental epilepsy in vitro: neuromodulating activity of anti-brain autoantibodies from rats exposed to electroconvulsive shock. | neuromodulating activity of anti-brain autoantibodies obtained from electroshocked (ecs) rats was tested on the neurons of isolated suboesophageal ganglion of the snail helix pomatia. in 16 out of 18 spontaneously active (pacemaker) neurons, ecs igg containing anti-brain autoantibodies induced short-lasting epileptiform discharges and membrane depolarization. membrane input resistance and time constant decreased, while membrane capacitance increased after addition of ecs igg. amplitude of evoked ... | 1991 | 1723059 |
| kaliotoxin, a novel peptidyl inhibitor of neuronal bk-type ca(2+)-activated k+ channels characterized from androctonus mauretanicus mauretanicus venom. | a peptidyl inhibitor of the high conductance ca(2+)-activated k+ channels (kca) has been purified to homogeneity from the venom of the scorpion androctonus mauretanicus mauretanicus. the peptide has been named kaliotoxin (ktx). it is a single 4-kda polypeptide chain. its complete amino acid sequence has been determined. ktx displays sequence homology with other scorpion-derived inhibitors of ca(2+)-activated or voltage-gated k+ channels: 44% homology with charybdotoxin (ctx), 52% with noxiustoxi ... | 1992 | 1730708 |
| presence of terminal n-acetylgalactosamine residues in subregions of the endoplasmic reticulum is influenced by cell differentiation in culture. | using helix pomatia lectin as a specific probe for terminal, nonreducing n-acetylgalactosamine residues, glycoprotein precursors bearing newly initiated o-linked oligosaccharides have been localized in the lumen of the endoplasmic reticulum and cis-golgi cisternae. this pattern contrasts with the detection of the terminal disaccharide galactose beta-1,3-n-acetylgalactosamine by arachis hypogaea lectin in middle and trans-golgi compartments, which are considered elongation sites for o-glycosylati ... | 1991 | 1748669 |
| carbohydrates in the guinea pig stria vascularis demonstrated with lectin-gold techniques. | soybean agglutinin (sba), helix pomatia agglutinin (hpa), ricinus communis agglutinin-ii (rca-ii), limax flavus agglutinin (lfa) and wheat germ agglutinin (wga) were employed to determine the localization of specific carbohydrates on thin sections of lowicryl k4m embedded guinea pig striae vasculares using the lectin-gold and glycoprotein-gold techniques. sba, hpa and rca-ii gold labeling was observed in many of the cytoplasmic vesicles, endosomes and apical tubules located in the supranuclear r ... | 1991 | 1763624 |
| use of thermospray liquid chromatography-mass spectrometry to aid in the identification of urinary metabolites of a novel antiepileptic drug, lamotrigine. | the use of thermospray liquid chromatography-mass spectrometry allowed the structural elucidation of a number of urinary metabolites of lamotrigine, 3,5-diamino-6-(2,3-dichlorophenyl)-1,2,4-triazine, formed after administering the drug to man, cynomolgus monkey and rabbit. this data when combined with the data obtained from high-performance liquid chromatography with radiochemical detection enabled us to determine the types and amounts of unchanged drug and metabolites excreted in urine by man a ... | 1991 | 1795036 |
| biochemical and serological properties of streptococcus uberis. | the strep-zym identification system, a combination of 23 enzymatic tests, allowed a rapid biochemical characterization of streptococcus uberis. the biochemical profiles of the s. uberis cultures clearly differed from those of s. agalactiae and s. dysgalactiae. serological grouping of s. uberis revealed polysaccharide antigens of groups e, g, p and u. some cultures of s. uberis demonstrated camp-like synergistic hemolytic activities on sheep blood agar and reacted specifically with the lectins of ... | 1991 | 1796686 |
| [how thick is the glycocalyx of human erythrocytes?]. | the aim of the experiment was to determine the thickness of the glycocalyx of human erythrocytes of the blood group a. for that purpose, the distance between the electron dense contrasted lipid layer of the plasmalemma and gold sol particles loaded with helix pomatia lectin was determined. the mean thickness of the glycocalyx under this conditions was 5.9 nm. | 1991 | 1801510 |
| defining the specificity of anti-a/b igm/g antibodies with different antigens and lectins. | the specificity of anti-histo blood group a/b antibodies is defined by the antigen used for their production and selection (in the case of mouse monoclonal antibodies, mabs) or by the antigens present on various intestinal bacteria (for polyclonal human igm and/or igg, phabs). absorption experiments with red blood cells and free antigen have been used earlier to define specificity; here we use a/b substances of human, animal and synthetic origin as well as known reactivities of mabs or lectins a ... | 1991 | 1862641 |
| a 58-kda resident protein of the cis golgi cisterna is not terminally glycosylated. | a 58-kda golgi protein (gp58) was previously identified and found to be concentrated in cis golgi cisternae in several cell types (saraste, j., palade, g.e., and farquhar, m.g. (1987) j. cell biol. 105, 2021-2029). in this study the protein was partially purified from rat pancreas and mouse myeloma cells in order to characterize its oligosaccharides. it migrated on sodium dodecyl sulfate-polyacrylamide gels as a 57-58-kda doublet under reducing conditions or as a single approximately 116-kda ban ... | 1991 | 1894639 |
| biosynthesis in vitro of glca beta 1-3nlcose4cer by a novel glucuronyltransferase (glcat-1) from embryonic chicken brain. | a novel glucuronyltransferase (glcat-1) has been detected in embryonic chicken brains. this enzyme catalyzes the biosynthesis in vitro of glucuronic acid containing glycolipids starting from neolactotetraosylceramide (nlcose4cer) and neolactohexaosylceramide (nlcose6cer). the activity is present primarily in the golgi-rich membrane fraction and can be extracted (60%) from the membrane using a neutral detergent, nonidet p-40, at ph 7.0. the detergent-solubilized glcat-1 is stable (70%) at -20 deg ... | 1991 | 1900517 |
| glycoconjugates in the human fetal endolymphatic sac as detected by lectins. | the distribution of glycoconjugates in the 11 to 16 weeks old human fetal endolymphatic sac (es) was analyzed using six biotinylated lectins; wheat germ agglutinin (wga), abrus precatorius agglutinin (apa), ulex europaeus agglutinin i (uea-i), ricinus communis agglutinin 120 (rca120), helix pomatia agglutinin (hpa), concanavalin a (cona). in the 11 week old human fetus, fluorescent reactions with wga, apa, rca120 and cona were detected in the es. there was almost no reaction with hpa and uea-i. ... | 1991 | 1919336 |
| species identification and characterization of an acanthamoeba strain from human cornea. | the isoenzyme pattern of an acanthamoeba, stock h-1, isolated from a patient with keratitis (krankenhaus heidberg, hamburg) was compared with that of two strains of a. quina-a. lugdunensis (302-2, 312-1), two stocks of a. lenticulata (45, 89-1) and one strain of a. rhysodes (302-1). the isolated stock showed glucose-6-phosphate dehydrogenase (g-6-pdh), beta-hydroxybutyric dehydrogenase (beta-hbdh), alcohol dehydrogenase (adh) and superoxide dismutase (sod) isoenzyme patterns similar to those of ... | 1991 | 1924251 |