| trichinella pseudospiralis infection in tasmanian wildlife. | | 1990 | 2375708 |
| infectivity of trichinella pseudospiralis isolated from carrion. | the reproductive capacity index for trichinella pseudospiralis infective larvae was similar for worms isolated from mouse carcasses on the day upon which mice were killed (day 0: 104.4 +/- 18.6 [mean +/- sd]) and on day 5 following mouse death (106.1 +/- 23.6), but was reduced for worms recovered from carcasses on day 10 postkill (pk: 22.7 +/- 5.7). larvae isolated from mouse carcasses held at 24 c after day 10 pk were not infective. the percentage of viable worms (tightly coiled or moving) isol ... | 1990 | 2213425 |
| alterations in the longevity and fecundity of adult trichinella pseudospiralis related to method of isolation of infective larvae. | the infectivity of trichinella pseudospiralis infective larvae was reduced significantly following exposure to low ph or a combination of 1% pepsin at low ph compared to that for larvae isolated in phosphate-buffered saline (pbs) at ph 7.0. reduction of host gastric ph by administration to mice of sodium bicarbonate solution in pbs was accompanied by an increase in the infectivity of larvae isolated in 1% pepsin/hcl (p/hcl) compared to that for worms inoculated into hosts given pbs alone. fewer ... | 1990 | 2161917 |
| trichinella spp.: differential expression of acid phosphatase and myofibrillar proteins in infected muscle cells. | major alterations are induced in muscle cells infected by either trichinella spiralis or trichinella pseudospiralis. to investigate the response of muscle to these infections we have analyzed the expression of acid phosphatase (acp, ec 3.1.3.2), adult skeletal muscle myosin heavy chain, and muscle tropomyosin proteins in infected mouse skeletal muscle cells. using t. spiralis-infected cells, we provide strong evidence that the tartrate-sensitive acp of these cells was synthesized by the infected ... | 1991 | 2015871 |
| immunization against schistosoma mansoni by using trichinella spiralis and trichinella pseudospiralis antigens. | the antigenic fraction of a cell free homogenate of each of trichinella spiralis and t. pseudospiralis were prepared to be used for induction of immunity against s. mansoni in albino mice. the results of the study showed that immunization with either of the two antigens induced reduction in worm load ranging from 80.48% to 87.86% in the different groups of the study. reduction in tissue egg count was also reported: liver egg count was reduced from 69.47% to 100%. intestinal egg count reduction r ... | 1991 | 1908495 |
| [the composition of the peripheral blood in white mice infected with trichinella spiralis and trichinella pseudospiralis]. | the study of the peripheral blood response in mice with an experimental t. spiralis infection from the 1 to the 60 days of the latter showed leucocytosis, lymphomonocytosis and neutropenia maximal at the 21--35 days of the infection along with hyperthrombocytosis and esr elevation, with the subsequent declination up to the end of the supervision. in experimental p. pseudospiralis infection lymphomonocytosis and esr levels were comparatively lower but eosinophilia was significantly higher. | 1991 | 1818247 |
| effect of acute versus chronic trichinella pseudospiralis infections on systemic cell-mediated immunity. | infection of the mouse with trichinella pseudospiralis is accompanied by pronounced suppression of host inflammatory response. this study examines the effects of infection with this parasite on several key elements in cell-mediated immunity. early down-regulation of host granulomatous response to subcutaneously implanted cotton string and delayed-type hypersensitivity (dth) response to trinitrochlorobenzene (tncb) was followed later during infection by normalization of these parameters compared ... | 1991 | 1787035 |
| stimulated chemotactic response in neutrophils from trichinella pseudospiralis-infected mice and the neutrophilotactic potential of trichinella extracts. | during infection with trichinella pseudospiralis a strong neutrophil response is evident in the peripheral circulation of the mouse. this study compared the chemotactic response of neutrophils from uninfected, t. pseudospiralis-infected and trichinella spiralis-infected mice to extracts from adult worms, newborn larvae and muscle-stage larvae of both species of parasite. the chemotactic response of neutrophils from t. pseudospiralis-infected mice to zymosan-activated mouse serum (zams) was signi ... | 1991 | 1757190 |
| the use of the polymerase chain reaction to identify porcine isolates of trichinella. | a method was developed to identify domestic isolates of trichinella using the polymerase chain reaction. oligonucleotide primers, based on the repetitive dna sequence (ppra) from the p1 isolate of trichinella, were used to amplify genomic dna from 13 domestic isolates and tested against sylvatic isolates of trichinella. pattern differences were observed among domestic isolates, indicating divergence of this repetitive sequence. the primers were specific for domestic trichinella as no amplificati ... | 1992 | 1738057 |
| characterization of a noncyst-forming isolate of trichinella from a wild boar in yugoslavia. | an isolate of trichinella obtained from a wild boar in yugoslavia did not form cysts in the musculature of its natural host. subsequent inoculation into experimental hosts demonstrated that some larvae became encysted only after extended time periods, whereas others remained unencapsulated. histological staining of larvae in the musculature demonstrated no deposition of collagen typically seen for trichinella spiralis spiralis, trichinella spiralis nativa, or trichinella spiralis nelsoni. the yu ... | 1991 | 1672709 |
| trichinella pseudospiralis overcomes innate resistance of the chinese hamster to trichinella spiralis. | infectivity of trichinella pseudospiralis in chinese hamsters was greater than that of trichinella spiralis. concurrent infection with these two species of parasite was accompanied by an increase in infectivity of t. spiralis. the infectivity of t. pseudospiralis, but not that of t. spiralis, was significantly influenced by host age. regardless of host age, t. pseudospiralis was more infective for the chinese hamster than was t. spiralis. early during infection, enteritis (myeloperoxidase activi ... | 1991 | 1664077 |
| taxonomic revision of the genus trichinella. | the analysis of genetic, biochemical, and biological data on about 300 trichinella isolates, reported in the literature, allows a taxonomic revision of this genus. we propose the recognition of 5 sibling species, trichinella spiralis (owen, 1835) sensu stricto; trichinella nativa britov and boev, 1972; trichinella pseudospiralis garkavi, 1972; trichinella nelsoni britov and boev, 1972 sensu stricto; and trichinella britovi n. sp., on the basis of biochemical and biological characteristics. trich ... | 1992 | 1635024 |
| biological characterization of trichinella isolates from various host species and geographical regions. | forty isolates of trichinella collected from 5 continents were compared for 7 biological characters: newborn larvae produced per female worm cultured in vitro at the seventh, eighth, and ninth day postinfection, host muscle nurse cell development time, reproductive capacity index in rats and chickens, and resistance of muscle larvae to freezing. the isolates also were compared by analyses of an environmental character of the location from which they were isolated: the isotherms for january and j ... | 1992 | 1635023 |
| allozyme analysis of trichinella isolates from various host species and geographical regions. | allozyme analysis was carried out on 152 trichinella isolates from synanthropic and wild animals and from humans; the isolates were collected from 5 continents. the analysis, involving 27 enzymes, revealed the presence of 8 distinct gene pools, termed t1-t8. four of the genetic groups represent the 4 previously proposed species: trichinella spiralis sensu stricto (t1), trichinella nativa (t2), trichinella nelsoni (t7), and trichinella pseudospiralis (t4). the other 4, t3, t5, t6, and t8 are dist ... | 1992 | 1635022 |
| allozymic and biological characters of trichinella pseudospiralis isolates from free-ranging animals. | to evaluate biological and biochemical variability in nonencapsulated trichinella isolates, biological and allozymic studies were conducted on isolates of trichinella collected from a raptoral bird (aquila rapax) and a fox (vulpes corsac) in kazakhstan and from a dasyurid marsupial (dasyurus maculatus) on the island of tasmania, australia. allozyme profiles of bird and marsupial isolates showed close similarity with the type isolate of trichinella pseudospiralis. the avian and fox isolates succe ... | 1992 | 1491304 |
| efficacy of albendazole against trichinella pseudospiralis and trichinella spiralis in mice. | albendazole (smith kline, beecham) in a dose of 20 mg/kg/day was given to b6c3f1 mice exposed to 300 larvae of t. pseudospiralis or t. spiralis. the drug was introduced on days 2, 3 and 4; or 4, 5 and 6; or 18, 19 and 20 after infection. it limited the intensity of intestinal and muscle phases of trichinellosis. given at the stage of maturation of adult forms and production of new-born larvae, the drug caused almost total elimination of both species from the intestines of mice and a decrease in ... | 1992 | 1299060 |
| [studies of the lymphatic system in mice infected with trichinella pseudospiralis as well as infected and stimulated by phytohaemagglutin (pha-p)]. | mice cfw were infected per os with trichinella pseudospiralis (150 larvae/mice). the mice were divided in two groups: nonstimulated and stimulated intraperitoneally with pha-p at dose 10 mg/kg body weight. the animals were killed 7, 14, 21, 28, 42, 56, 70 and 150 day post infection (d.p.i.). spleen et mesenteric lymph nodes were examined histopathologically (hematoxylin-eosin) and histochemically (nonspecific esterase reaction) for differentiation of t lymphocytes. the obtained results reveal th ... | 1992 | 1299055 |
| [morpho-physiologic criteria for the taxonomic independence of the species trichinella pseudospiralis garkavi, 1972]. | a taxonomic status of a new species of trichinella from procyon lotor l. described by v. l. garkavi in 1972 as t. pseudospiralis is discussed. two-month invasional larvae resemble in their size and structure 19-20-day larvae of typical t. spiralis. however, the ratio between their organs points not only to the delay in development but also to significant structural morphological differences. luminiscent microscopic analysis of muscular and intestinal forms of both species confirms this. impossib ... | 1977 | 865876 |
| [immunological factors in the host-parasite relationship. i. serum immunoglobulin levels in mice infected with trichinella pseudospiralis]. | | 1978 | 741740 |
| effect of intestinal flora on the course of infection and haematological changes in germfree and conventional mice exposed to trichinella pseudospiralis (garkavi, 1972). | | 1978 | 698864 |
| effect of intestinal flora on the immunogical response of germfree and conventional mice exposed to trichinella pseudospiralis (garkavi, 1972). | | 1978 | 698863 |
| [pathomorphology of skeletal muscles in birds infected with trichinella pseudospiralis]. | | 1978 | 664699 |
| [cholinesterase activity in muscle phase of trichinella pseudospiralis garkavi (1972) infection in mice]. | | 1978 | 664695 |
| b and t lymphocytes from peripheral blood in mice infected with trichinella spiralis and trichinella pseudospiralis. | | 1978 | 313234 |
| is trichinella pseudospiralis likely to be a human pathogen? | | 1978 | 78115 |
| muscle fiber selectivity of trichinella spiralis and trichinella pseudospiralis (1983). | the biceps, semimembranosus, biceps femoris, and soleus muscles of female rockland wistar mice infected with either 1,000 trichinella spiralis or 1,000 trichinella pseudospiralis larvae were removed on days 12, 14, 16, and 18 post-infection (pi), sectioned and stained histochemically for their myosin atpase activity. light microscopic examination of the sections revealed that larvae of t. spiralis invade only the slow twitch muscle fibers, and those of t. pseudospiralis invade both the fast twit ... | 1986 | 2426435 |
| trichinella pseudospiralis infection in gallus domesticus. i. intestinal and muscles phases. | | 1985 | 4072222 |
| trichinella pseudospiralis infection in gallus domesticus. ii. influence on host body weight, haematological and immunological parameters. | | 1985 | 4072223 |
| nonenzymatic isolation of trichinella pseudospiralis infective l1 larvae at ph 7.4. | over half of the number of trichinella pseudospiralis infective l1 larvae recovered from host carcasses by pepsin-hcl digestion were isolated from homogenized carcasses incubated in hbss. more worms isolated by the latter method were viable compared to those isolated by pepsin-hcl digestion. when host carcasses infected with t. pseudospiralis were diced into pieces and incubated in hbss, 30% more worms were recovered than from homogenized carcasses incubated in hbss as above, and the majority of ... | 1989 | 2493089 |
| an analysis of trichinella pseudospiralis excretions and study of their effect on striated muscles of mouse. | the causes of changes observed after infection of muscles with trichinella pseudospiralis larvae were studied. the changes are not only regressive, but also proliferative (activation and mitosis of satellite cells and formation of new myotubes). by means of high-performance liquid chromatography (hplc) and an original method it was found that t. pseudospiralis larvae excrete into their environment among others a great amount of n-butylamine. a direct application of n-butylamine into the gluteal ... | 1989 | 2527792 |
| regeneration in mouse skeletal muscle injured by trichinella larvae. | during the infection caused by trichinella pseudospiralis serious damage to muscles is partly compensated with regeneration process. short, thin fibrillae with central position of the nuclei--regenerating myotubes originate 20--40th day post infection in endomysial tubes remaining after damaged muscle fibres, left by migrating larva. on the 10th day post infection in the vicinity of moving larva activation of nuclei and increased origin of satellite cells under basal membrane occur. with develop ... | 1989 | 2527793 |
| mitochondrial atp-ase as a measure of uncoupling of rat muscle mitochondria in experimental infection with trichinella spiralis and trichinella pseudospiralis. | changes in a bioenergetic state of trichinella spiralis and trichinella pseudospiralis infected rat and mouse muscle mitochondria were evaluated enzymatically, and in both infections 3-4-fold increase of mitochondrial, mg++-stimulated atp-ase (ec 3.6.1.3) was observed. looking for the dynamics of those bioenergetic changes in t. pseudospiralis infected rat and mouse muscle mitochondria 1-2 weeks, pi, the 5-6-fold stimulation of matp-ase activity, followed by a significant drop between the week 3 ... | 1989 | 2530698 |
| infection of the chinese hamster with trichinella pseudospiralis. | a mean of 2,862 muscle larvae was recovered on day 45 postinfection (pi) from the total body musculature of chinese hamsters infected with 498 trichinella pseudospiralis. infection of the chinese hamster with 494 trichinella spiralis resulted in recovery of a mean of 225 muscle larvae on day 45 pi. the reproductive capacity index for t. pseudospiralis was 5.74, whereas that for t. spiralis was 0.46 in this host species. | 1989 | 2614596 |
| a mechanism for anti-asialo gm 1 antibody-induced anaphylactoid response in mice infected with trichinella pseudospiralis. | intravenous injection of anti-asialo gm 1 antibody into mice infected with trichinella pseudospiralis resulted in rapid acute illness or death accompanied by a dramatic rise in hematocrit values in these animals. the described antibody-induced changes were reversible by intravenous infusion of hanks' balanced salt solution (hbss). these effects were not seen in uninfected mice or in trichinella spiralis-infected mice injected with anti-asialo gm 1 antibody. viability of t. spiralis or t. pseudos ... | 1989 | 2795381 |
| trichinella pseudospiralis in tasmanian native fauna. | | 1989 | 2818355 |
| effect of vitamins on trichinella pseudospiralis garkavi, 1972 infection in mice. | the effects of vitamins a, b complex, e, and ade on the eosinophilia, body weight, number of obtained larvae and distribution of larvae of trichinella pseudospiralis were examined in mice. the increased eosinophilia started to appear in the majority of infected mice from day 7 p.i., the maximum being around day 21 p.i. the highest values of eosinophilia were observed in the mice after application of vitamin b complex. in spite of considerable individual differences, the greatest decrease in body ... | 1987 | 2962915 |
| allergenic cross-reactivity of several strains of trichinella in mice and rats by the passive cutaneous anaphylaxis technique. | the cross-reactivity of antiparasite ige antibody responses induced by allergenic antigens obtained from trichinella spiralis and trichinella pseudospiralis was determined by the passive cutaneous anaphylaxis (pca) technique in bcf1 mice infected with t. spiralis, trichinella nelsoni, trichinella nativa, and t. pseudospiralis and in rats infected with t. spiralis and t. pseudospiralis. our results demonstrate that when the antigen used for the pca challenge is derived from muscular l1 larvae of ... | 1988 | 3338853 |
| trichinella pseudospiralis larvae express natural killer (nk) cell-associated asialo-gm1 antigen and stimulate pulmonary nk activity. | natural killer (nk) cell function was evaluated in mice infected with either trichinella pseudospiralis or t. spiralis larvae. t. pseudospiralis-infected mice consistently demonstrated augmented pulmonary nk cell-mediated clearance of yac-1 tumor cells in vivo but failed to display enhanced splenic nk cell-mediated lysis of the same tumor cells in vitro. attempts to alter nk cell function in vivo by the injection of anti-asialo-gm1 antibody resulted in anaphylaxis and death of the hosts infected ... | 1988 | 3356458 |
| a role for elevated plasma corticosterone in modulation of host response during infection with trichinella pseudospiralis. | suppression of host inflammatory response in mice infected with trichinella pseudospiralis was associated with host plasma corticosterone levels significantly higher than those seen in uninfected mice or in mice infected with t. spiralis. increases in the population of mitochondria and depletion of lipid droplets in cells of the zona fasciculata were seen in the adrenals of mice infected with t. pseudospiralis. elevations in enteritis, myositis and myocarditis accompanied 100% mortality in adren ... | 1988 | 3374961 |
| [trichinosis in mice irradiated and subsequently infected by trichinella pseudospiralis larvae]. | | 1987 | 3452942 |
| a comparison of the surface and secretions of trichinella pseudospiralis and t. spiralis. | intact, viable adults, infective and newborn larvae of trichinella pseudospiralis were surface labelled with 125i by the chloramine t method and labelled proteins were compared with those obtained from equivalent stages of t. spiralis. electron-microscope autoradiography determined that labelled proteins were restricted to the cuticle for all stages of both isolates. comparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (sds-page), using thin gradient gel slab ... | 1986 | 3748610 |
| [effect of trichinella pseudospiralis and t. spiralis infection on intestinal absorption in rats]. | | 1985 | 3834695 |
| [genetically determined immunity against intestinal nematodes: intestinal phase of trichinella pseudospiralis infection in inbred nih mice]. | | 1985 | 3938909 |
| modulation of host response by trichinella pseudospiralis. | measurement of myeloperoxidase activity in the muscles of mice infected with trichinella pseudospiralis, t. spiralis or both helminths allowed quantitation of host inflammatory response to the parasite. infection of the host with t. pseudospiralis alone was accompanied by less inflammation in host diaphragm muscle than was the case in hosts infected with t. spiralis alone. a dramatic reduction in inflammation around s.c. implanted cotton string was observed in mice infected with t. pseudospirali ... | 1985 | 4011298 |
| immune responses to trichinella pseudospiralis and trichinella spiralis in mice. | | 1985 | 4030207 |
| [makeup of potential hosts of the trichina trichinella pseudospiralis]. | | 1974 | 4449651 |
| trichinella pseudospiralis in man. | | 1993 | 8101314 |
| [trichinella pseudospiralis infestation of swine and cats]. | | 1973 | 4783620 |
| the influence of some anthelmintics on the bioenergetic metabolism of trichinella spiralis and trichinella pseudospiralis. | | 1984 | 6466367 |
| intestinal trichinella spiralis and trichinella pseudospiralis in germfree and conventional mice. | by indirect hemagglutination (iha) test in germfree (gf) and conventional (cv) mice, better antibody response was found in cv mice infected with 200 t. spiralis larvae. in contrast, when 500 larvae were used for infection, the response of gf mice exceeded that of cv controls. humoral responses of gf and cv mice infected with single or mixed t. spiralis or t. pseudospiralis larvae were examined by iha test with the use of both antigens and by determination of g1, a and m immunoglobulins. cellular ... | 1983 | 6657977 |
| the open-field behaviour of mice infected with trichinella pseudospiralis. | the behaviour of mice exposed to graded doses of trichinella pseudospiralis larvae was monitored in an open-field situation. ambulatory activity declined briefly during the acute phase of the infection (day 11) but regained pre-infection levels 1 week later. animals receiving heavy doses of t. pseudospiralis exhibited a mild increase in the level of ambulatory activity during the chronic phase of the infection. the infections did not affect the level of exploratory activity. the effects of the t ... | 1984 | 6739128 |
| [pathomorphology of rat muscle tissue in the course of trichinella pseudospiralis infection]. | | 1980 | 7032078 |
| the reaction of mouse muscle cells during simultaneous trichinella spiralis and trichinella pseudospiralis infection. | | 1980 | 7246268 |
| lymphocytotoxicity of mouse sera in the course of experimental infection with trichinella spiralis and trichinella pseudospiralis. | | 1980 | 7272822 |
| comparative analysis of mobility and ultrastructure of intramuscular larvae of trichinella spiralis and trichinella pseudospiralis. | the muscle phase of trichinella spiralis and trichinella pseudospiralis was studied by using scanning electron microscope techniques, closed circuit television, and video tape recording. the complete absence of any cyst structure including the pseudocapsule allows t. pseudospiralis to move freely between the muscle layers. its rate of activity, measured as distance moved between two points, was 2.83 mm/min compared to that of the encysted t. spiralis larvae which was 0.237 mm/min. there was an a ... | 1981 | 7299578 |
| a comparative study of trichinella spiralis (owen, 1835) and trichinella pseudospiralis (garkavi, 1972). | | 1981 | 7328464 |
| abnormal weight gain in mice, induced by trichinella pseudospiralis. | | 1980 | 7397613 |
| comparative study on polypeptide patterns of larvae of trichinella isolates by two-dimensional electrophoresis. | the two-dimensional patterns (isoelectrofocusing-ief/polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate-sds) of s3 fractions of muscle larvae of four trichinella isolates were compared. the comparative study concerned six groups of polypeptides. it was observed that the garkavi isolate of trichinella pseudospiralis was clearly different from the other isolates, and it showed the simplest ief/sds polypeptide pattern. the c-76 isolate of t. nelsoni had only four of the s ... | 1987 | 3668212 |
| the enzyme-linked immunosorbent assay (elisa) for determination of specific antibodies in mice infected with trichinella spiralis or trichinella pseudospiralis. | | 1986 | 3788171 |
| trichinella pseudospiralis as a model for the "in vitro" screening of anthelmintics. | | 1986 | 3788175 |
| comparative activity of various anthelmintics in relation to the time of muscular infection by trichinella pseudospiralis. | | 1986 | 3788176 |
| genetic distance between trichinella pseudospiralis and other trichinellae by single oral immunization. | | 1985 | 4072210 |
| compared genetic distance of trichinella pseudospiralis with other trichinellae isolations by hyperimmunization. | | 1985 | 4072211 |
| [monoamine oxidase (mao) activity in the blood, spleen, mesenteric lymph node and small intestine of mice with trichinella pseudospiralis infection]. | | 1985 | 4072216 |
| sense organs of the nematodes trichinella pseudospiralis garkavi, 1972 and t. nativa britov et boev, 1972. | the structure and distribution of the sense organs of the head and the tail of two day-old trichinella species (females) have been determined by transmission (tem) and scanning (sem) electron microscopy. in both species occur 16 cephalic sense organs, which contain three modified dendritic processes, in addition to the amphids. the nerve cell bodies are situated in the area above the nerve ring. the amphid contains ten dendritic processes. the sense organs open to the external environment by por ... | 1984 | 6745797 |
| suppression of transplant immunity in experimental trichinellosis. | skin allograft rejection in balb/c and c57bl/6j mice following experimental infection with 300 larvae of trichinella spiralis or trichinella pseudospiralis was studied. skin grafts from normal c57bl/6j mice were transplanted to infected balb/c mice and vice versa at days 3, 10, 20 and 30 post-infection. the clinical criteria for graft rejection, scarring and graft falling, were followed. the results indicated that t. spiralis and t. pseudospiralis infections induced a significant delay in graft ... | 1995 | 7554818 |
| detection of trichinella pseudospiralis antigen in the enteral phase in experimentally infected mice. | the distribution of t. pseudospiralis antigen in tissue sections of the intestine of infected mice was studied at different intervals (10, 21, 35 hours and 10 days post-infection (p.i.). immunocytolocalization of antigen in the intestine was done by peroxidase antiperoxidase staining technique. positive staining reaction of the enterocytes was noted in all durations examined. the mononuclear cells of the lamina propria were positively stained at 10 hrs p.i. while patchy staining of muscularis mu ... | 1995 | 7665942 |
| identification of trichinella pseudospiralis from a human case using random amplified polymorphic dna. | a human case of infection by trichinella pseudospiralis has recently been described. some morphologic anomalies of the muscle larvae, however, raise the possibility of an incorrect taxonomic attribution. a molecular taxonomic approach has therefore been applied for the identification of the parasite. random amplified polymorphic dnas were obtained from a single larva extracted from a muscle biopsy of the suspected case of t. pseudospiralis infection, and compared with those derived from 27 refer ... | 1995 | 7677222 |
| [a comparative electrophoretic analysis of the isoenzymes in "reference" and natural strains of trichinella]. | in four enzymes among eight ones examined the differences were revealed in the intensity of staining of certain zones (g-6-p, es) as well as in electrophoretic mobility of some loci (pgd, rgm) between the capsule forms of trichinellae and trichinella pseudospiralis. as a result, the validity of isolation of t. pseudospiralis garkavi, 1972 is confirmed. | 1994 | 7816504 |
| trichinella pseudospiralis in humans: description of a case and its treatment. | the first known human case of trichinella pseudospiralis myositis is described. a 33 years old woman reported 5 years of relatively mild symptoms of tiredness, muscle fatigue and muscle pain after exercise. she had minimal proximal weakness. creatinine kinase was significantly elevated, and muscle biopsy showed polymyositis and trichinella larvae. steroid treatment dramatically worsened the weakness. treatment with albendazole led to complete resolution of symptoms and laboratory abnormalities. ... | 1994 | 8036673 |
| the effect of trichinella spiralis and trichinella pseudospiralis on the mechanical properties of mammalian diaphragm muscle. | the isometric mechanical properties of diaphragm muscle were studied in mice infected with either trichinella spiralis or trichinella pseudospiralis. measurements of muscle stress were taken at 15, 20, 30 and 40 days post-infection. infected diaphragm muscle showed a significant (p < 0.001) reduction in muscle stress during both twitch and tetanic contractions when compared with muscle from control animals. t. spiralis caused a significant reduction in resistance to muscle fatigue. the consequen ... | 1994 | 8058363 |
| changes in host muscles induced by excretory/secretory products of larval trichinella spiralis and trichinella pseudospiralis. | excretory/secretory (es) products obtained by in vitro culture of infective-stage larvae of trichinella spiralis and t. pseudospiralis were injected intramuscularly at various intervals into mice. mini-osmotic pumps containing t. spiralis es products were also implanted subcutaneously and intraperitoneally into rats. the introduction of es materials into muscles elicited extensive lesions which included dissolution of myofibres, mobilization of mononuclear and polymorphonuclear leucocytes, angio ... | 1994 | 8159465 |
| [comparison of numbers of active lymphocytes b in spleen of mice infected with trichinella pseudospiralis or trichinella spiralis]. | the experiments were performed on balb/c mice (male, 20 g body weight). the mice were infected 23 days before the experiment by peroral application of about 100 trichinella larves. control group consisted of not infected mice. the number of lymphocytes b producing antibodies was determined according to cunningham and szenberg. five days before performance of the test, all mice were immunized by peritoneal application of sheep erythrocyte suspension. after that the mice were sacrificed, spleen wa ... | 1993 | 8189819 |
| contractile characteristics of the flexor muscle of mice infected with trichinella spiralis, t. nativa or t. pseudospiralis. | a comparative analysis of skeletal muscle isometric contractile characteristics was performed in vivo on the flexor muscle of mice infected 6 mo earlier with 400 larvae of trichinella pseudospiralis, trichinella spiralis, or trichinella nativa. the control group consisted of age- and sex-matched uninfected mice. the mice were injected with 0.1 ml of 50% urethane in saline, and the skin of the left hind limb was cut open longitudinally. the exposed flexor muscle was freed from the adjacent tissue ... | 1994 | 8195936 |
| dynamics of rat muscle mitochondria uncoupling in trichinellosis. | parallel oxygraphic measurements of respiratory control index (rci) and spectroscopic measurements of the activity of two mitochondrial enzymes from rat muscle, mtatpase and succinate dehydrogenase were performed. in trichinella spiralis infection, in comparison with trichinella pseudospiralis infection, a delayed stimulation of mtatpase in 3rd week p.i. was observed and the degree of stimulation was weaker. a second peak of mtatpase stimulation appeared at the beginning of the encystation phase ... | 1993 | 8225761 |
| superoxide dismutase of trichinella spiralis and trichinella pseudospiralis larvae. | the aim of this study was to compare the in vitro and in vivo activities of superoxide dismutase, an enzyme acting against free-radicals, in trichinella spiralis and t. pseudospiralis isolates. in vitro, the activity of the superoxide dismutase in t. pseudospiralis was determined to be about two times higher than in t. spiralis. only one band representing superoxide dismutase was found by electrophoretic analysis. in vivo, in muscles infected with t. spiralis, a strong histochemical reaction in ... | 1993 | 8256096 |
| biochemical characterisation of trichinella spiralis and t. pseudospiralis stichocyte antigens. | one and two dimensional western blots of trichinella spiralis and trichinella pseudospiralis extracts were probed with a monoclonal antibody (7c2c5) which recognizes molecules found in the stichocytes. three major antigens were revealed in excreted:secreted (e/s) preparations at approx mr 45, 53 & 60 x 10(3). the mr 45 x 10(3) molecule was the most abundant secreted antigen, on two dimensional analysis it resolved into many isoforms with a pi range 4.5-6.4 in t. spiralis and into four isoforms p ... | 1993 | 8298662 |
| identification of trichinella isolates from naturally infected stray dogs in egypt. | larvae of trichinella species recovered from the diaphragms of 2 stray dogs killed during a governmental antirabies campaign in cairo, egypt, were fed to white mice for production of adult worms and larvae for morphological and isoenzyme studies. comparisons were made with reference species of trichinella spiralis, trichinella nelsoni, trichinella nativa, and trichinella pseudospiralis. results indicated that the 2 trichinella specimens from the dogs were morphologically and biochemically identi ... | 1994 | 8308650 |
| trichinella spiralis and the concept of niche. | trichinella spiralis is an intracellular parasite as both a larva and an adult. the first-stage larva lives in a modified portion of a skeletal muscle cell, the nurse cell, and can reside there for the life span of the host. adult worms occupy a nonmembrane-bound portion of columnar epithelium, living there as intramulticellular parasites. the newborn larva is the only nonintracellular stage, living free in the circulation. trichinella spiralis induces modifications in each of its intracellular ... | 1993 | 8331468 |
| scanning electron microscopic study of trichinella pseudospiralis muscle phase after modulation of host response using an immunostimulant drug. | in a previous study, evident collagen deposition around t. pseudospiralis larvae was noticed after administration of levamisole to infected mice. the present investigation was undertaken to confirm these findings. scanning electron microscopic examination revealed multiple clear cystic formations on the surface of the diaphragm muscle. the present investigation was undertaken to confirm these findings. scanning electron microscopic examination revealed multiple clear cystic formations on the sur ... | 1993 | 8376867 |
| enteral and parenteral phases of trichinella nativa and trichinella pseudospiralis in the deer mouse, peromyscus maniculatus. | trichinella nativa and trichinella pseudospiralis infections in a wild rodent host, the deer mouse (peromyscus maniculatus), were characterized. forty-six percent of 400 inoculated t. nativa were recovered on day 4 postinoculation (pi); 77% and 23% were found in the small and large intestines, respectively. thirty-one percent of the worms recovered on day 4 remained in the large intestine beyond day 20 pi. worms were embedded in the mucosa of the small intestine, cecum, and colon. females recove ... | 1993 | 8410546 |
| modification of the ultrastructure of the muscle larva of trichinella pseudospiralis following exposure to acidified pepsin solution. | examination of the cuticle of trichinella pseudospiralis by transmission electron microscopy revealed an epicuticle, exocuticle, and mesocuticle, each divided into several layers. the epicuticle consisted of an outermost thin plasmalemmalike infracuticular material covering an inner trilaminar membrane. the exocuticle was granular and could be divided into 2 regions on the basis of density. the mesocuticle was fibrillar and 3 regions could be distinguished based on the orientation of fibrils. th ... | 1993 | 8437055 |
| modulation of host response during infection with trichinella pseudospiralis using immuno-stimulant drug. | infection of the mouse with trichinella pseudospiralis is accompanied by pronounced suppression of host inflammatory response. this study examines the effect of infection with this parasite on one major component of cell mediated immunity: delayed type hypersensitivity (dth) reaction and the effect of administration of immunostimulant drug (levamisole) on host response to t. pseudospiralis infection. serum cortisol levels and the response of skin test to phytohaemagglutinin were used to construc ... | 1993 | 8482856 |
| isolation and characterization of trichinella pseudospiralis garkavi, 1972 from a black vulture (coragyps atratus). | a nematode from the genus trichinella was observed in histological sections of breast and tracheal muscles from a black vulture coragyps atratus from alabama. larvae obtained from breast muscle tissue that had been refrigerated for 8 days were infectious for laboratory mice. no nurse cell was observed around larvae in the black vulture or in experimentally infected mice examined 7 or 9 wk postinoculation. the identity of the parasite as trichinella pseudospiralis was confirmed by dna hybridizati ... | 1995 | 8544065 |
| variations in microsatellite sequences provide evidence for population differences and multiple ribosomal gene repeats within trichinella pseudospiralis. | enzymatic amplification of expansion segment 5 sequences within domain iv of the large subunit ribosomal dna generated distinct results among geographical isolates of trichinella pseudospiralis from russia, north america, and australia from both avian and mammalian hosts. discrete, multiple dna fragments ranging in approximate size from 285 to 360 bp were observed within and among each of the isolates tested. polymerase chain reaction performed on individual adult parasites from each isolate res ... | 1996 | 8691360 |
| trichinella spiralis and trichinella pseudospiralis induce collagen synthesis by host fibroblasts in vitro and in vivo. | immunoperoxidase staining of muscle infected with trichinella spiralis for murine collagen types i and iv provided both qualitative and quantitative evidence of extensive synthesis of both types of collagen by fibroblasts in infected muscle compared to that seen in uninfected muscle. moreover, fibroblasts in muscle infected with t. pseudospiralis, a nonencapsulating species, showed significantly less staining for both types of collagen compared to muscle from mice infected with t. spiralis. anal ... | 1995 | 8719950 |
| detection of trichinella pseudospiralis antigen in the parenteral phase in experimentally infected mice. | antigen distribution of t. pseudospiralis in muscles was studied at 10 days, 2,3 and 5 weeks post infection. immunocytolocalization of antigen in the muscle was performed by peroxidase antiperoxidase (pap) and immunogold silver staining techniques. weak positive reaction of the stichosome and the cuticle was observed at 10 days post-infection which increased to attain its highest level in the fully infective stage larvae 3 and 5 weeks post infection. the intensity of staining reaction in the cyt ... | 1996 | 8721224 |
| detection of circulating trichinella pseudospiralis antigen by enzyme linked immunosorbant assay (elisa). | a modified double antibody sandwich elisa was used as a sensitive technique for demonstrating the appearance of circulating antigen of t. pseudospiralis in the sera of infected mice. the sera were tested at 5 days, 2, 4, 6, 7, 8, 10 and 12 weeks p.i. the antigen was first detected on the 5th day p.i., its level increased to reach the 1st peak at 4 weeks, then declined at 6 weeks. a second peak was observed at 8th weeks followed by a sharp decline to give negative od reading at 10th week and incr ... | 1996 | 8721242 |
| trichinella pseudospiralis secretes a protein related to the trichinella spiralis 43-kda glycoprotein. | a 43-kda secreted glycoprotein from the intracellular parasitic nematode trichinella spiralis has been considered as a factor involved in the formation of the nurse cell in infected muscle. the closely related intracellular parasitic nematode trichinella pseudospiralis that also infects muscle cells, does not form nurse cells and was thought not to secrete the 43-kda glycoprotein. this implied a unique role for the 43-kda glycoprotein in t. spiralis infection and supported the hypothesis of invo ... | 1996 | 8813674 |
| [the comparative morphology of trichinella spiralis and trichinella pseudospiralis]. | the comparative study of the morphology of invasive larvae and mature males and females t. spiralis and t. pseudospiralis at the head end of the parasites has revealed 14 papillae which are located in 3 circles and 2 which are placed bilaterally, 2 amphidae, and a lanceolar stiletto with a spicular cutting edge. there are no differences in the structure of the heads of these two trichinella species. the larval cuticle is decorated only with cross folds while longitudinal small-sized folds which ... | 1997 | 9182188 |
| immunological characteristics and localization of the trichinella spiralis glutathione s-transferase. | trichinella spiralis glutathione s-transferase (tsgst) was isolated from crude extracts of l1 larvae by glutathione-affinity chromatography. two closely migrating polypeptides with molecular masses of 28.5 and 28 kda were identified by electrophoresis. three isoforms of pi 5.6, 5.8, and 6.0 were detected by isoelectric focusing. purified tsgst showed a low transferase activity as measured with 1-chloro-2,4-dinitrobenzene; glutathione peroxidase activity was also demonstrated using cumene hydrope ... | 1997 | 9267403 |
| first outbreak of human trichinellosis caused by trichinella pseudospiralis. | this is the first report of an epidemic of human infection with trichinella pseudospiralis. an outbreak of trichinellosis affecting 59 individuals, of whom one died, occurred in southern thailand during 1994-1995. the source of this epidemic was raw pork from a wild pig that was distributed to villagers by a local hunter. the most striking clinical features among 50 individuals who could be followed were muscular swelling, myalgia, and asthenia persisting for > 4 months. these were associated wi ... | 1998 | 9455518 |
| differences and similarities between trichinella spiralis and t. pseudospiralis in morphology of stichocyte granules, peptide maps of excretory and secretory (e-s) products and messenger rna of stichosomal glycoproteins. | the ultrastructure of muscle larvae of trichinella pseudospiralis was studied by electron microscopy. the overall structure of muscle larvae of t. pseudospiralis resembled that of t. spiralis except for the stichocyte granules. t. pseudospiralis had at least 3 kinds of stichocyte granules distinguishable from each other by their shape, size and inclusions. the granules had some resemblance to alpha granules or beta granules of t. spiralis, but no resemblance to gamma granules. in favour of these ... | 1998 | 9481775 |
| characterization of eleven antigenic groups in trichinella genus and identification of stage and species markers. | several monoclonal antibodies (mabs) were raised against the l1 muscle stage (l1m) of trichinella spiralis (ts) and trichinella pseudospiralis (tp). western blot analysis of various antigenic preparations established that mabs described by different authors recognized 8 antigenic fractions (tsl1-tsl8) in crude extracts of infective larvae. the tsl1 fraction was immunodominant and present on the cuticle of different parasite stages. mabs against trichinella t5 (t5) and ts were selected in order t ... | 1997 | 9488876 |
| emerging infections in australia. | over the last 10 years, novel infectious agents including equine morbillivirus, lyssavirus, barmah forest virus, rickettsia honei and two as-yet-unnamed bunyaviruses have been identified as causes of human disease in australia. previously described agents, such as japanese b encephalitis virus, dengue virus, ross river virus, orientia tsutsugamushi, rickettsia australis, burkholderia pseudomallei, mycobacterium ulcerans and trichinella pseudospiralis, have increased their geographical distributi ... | 1997 | 9494666 |
| comparison of the inflammatory responses of mice infected with american and australian trichinella pseudospiralis or trichinella spiralis. | this study was designed to determine if the tasmanian devil isolate of trichinella pseudospiralis suppressed inflammation as does the original isolate. while adult worm numbers were similar in all groups, lower enteritis occurred in devil isolate-infected mice compared with mice infected with the original isolate of t. pseudospiralis or with trichinella spiralis. diaphragm muscle inflammation was greater in t. spiralis-infected than in mice infected with either isolate of t. pseudospiralis or co ... | 1998 | 9512999 |
| [the effect of an invasion by trichinella spiralis and trichinella pseudospiralis on the ultrastructure of skeletal muscle tissue in the white mouse]. | the ultrastructure of striated muscular tissue was studied in experiments with three groups of white mice: those infected with t. spiralis, t. pseudospiralis, and in those uninfected (controls). there was a heterogeneity of changes in the striated muscular tissue during 35-day infection. deep damages to the contractile, trophic, energetic, supporting, and membranous apparatuses of muscle fibers are responsible for subcellular structural changes. at the same time compensatory and adaptative proce ... | 1998 | 9608200 |
| [genetic variability of trichinella spiralis oven, 1835, and trichinella pseudospiralis garkavi, 1972, detected by polymerase chain reaction with random primers]. | dna polymorphisms in two parasitic nematode species, trichinella spiralis oven, 1835, and trichinella pseudospiralis garkavi, 1972, were revealed via random amplification of polymorphic dna by the polymerase chain reaction (rapd pcr). the diagnostic value of seven 10-bp oligonucleotide primers was evaluated, and the extent of the homology between the genomes of the two species was estimated. the intraspecific variation of rapd markers was revealed in larvae of both species isolated from experime ... | 1998 | 9612694 |
| the detection of trichinella with polymerase chain reaction (pcr) primers constructed using sequences of random amplified polymorphic dna (rapd) or sequences of complementary dna encoding excretory-secretory (e-s) glycoproteins. | diagnostic pcr primers for trichinella were constructed. twelve pairs of primers were designed based on the sequences of random amplified polymorphic dna, and 4 pairs of primers were designed based on the reported sequences of complementary dna encoding excretory-secretory glycoproteins. with these primers, 31 samples of dna from different strains of trichinella including 5 species (trichinella spiralis, trichinella britovi, trichinella nativa, trichinella nelsoni and trichinella pseudospiralis) ... | 1998 | 9778640 |
| trichinella pseudospiralis: x-ray analysis of the infected muscle cells and the muscle larva compartments after exponential growth of the larva in situ. | the distribution of nutrients such as na, cl, k, p, s, and ca was investigated by energy-dispersive x-ray microanalysis (edxa) on bulk-frozen mouse muscles infected with trichinella pseudospiralis. in an attempt to gain information as to whether muscle larvae would modify the element status within muscles of their host. significant increases in phosphorus concentrations within nurse cells and internal cells of the larva, e.g., the somatic muscle cells and the stichocytes, were shown. this could ... | 1998 | 9797058 |