| inhibitory effect of bursaphelenchus mucronatus (nematoda: aphelenchoididae) on b. xylophilus boarding adult monochamus alternatus (coleoptera: cerambycidae). | inhibitory effects of bursaphelenchus mucronatus on the number of b. xylophilus carried by an adult monochamus alternatus were investigated using artificial pupal chambers. when pupal chambers were infested with either b. xylophilus or b. mucronatus, the load of b. xylophilus onto the beetle was greater (p < 0.001) than that of b. mucronatus. however, within the pupal chamber there was no difference in the abundance of the third-stage dispersal juveniles, which would molt to the fourth-stage dis ... | 2004 | 19262792 |
| cryopreservation of the pinewood nematode, bursaphelenchus spp. | populations of three isolates of bursaphelenchus xylophilus, the pinewood nematode, and one of b. mucronatus were treated with three cryoprotectants at -70 c for 24 hours followed by deep freezing at -180 c in liquid nitrogen for different periods of time. a solution of 15% glycerol, 35% buffer s, and 50% m9, or 1% aqueous solution of dimethylsulfoxide (dmso), or a mixture of 60% m9 and 40% s buffer were used as cryoprotectants. a significantly larger number of juveniles than adults survived dee ... | 1991 | 19283151 |
| temperature-mediated behavioral relationships in bursaphelenchus xylophilus, b. mucronatus, and their hybrids. | the influence of temperature on reproduction and movement was examined for seven geographic isolates of bursaphelenchus xylophilus, three of b. mucronatus, and two of their interspecific hybrids. all nematode isolates tended to be more active and fecund the higher the temperature, with the isolates of b. xylophilus reaching a reproductive peak at higher temperatures than isolates of b. mucronatus. most isolates of b. xylophilus and b. mucronatus did not produce significantly more progeny at high ... | 1992 | 19283200 |
| change in water status of pinus thunbergii parl. inoculated with species of bursaphelenchus. | maximum and minimum xylem pressure potentials of needles were measured to evaluate water status of pinus thunbergii parl. after inoculation with the virulent or avirulent populations of bursaphelenchus xylophilus or b. mucronatus. in virulent b. xylophilus-inoculated pines, the water status changed abruptly and needle chlorosis occurred by day 29 after inoculation. similar changes were not seen in b. mucronatus-inoculated and uninoculated control pines. oleoresin flow ceased in virulent b. xylop ... | 1990 | 19287700 |
| characterization of a nonparasitic isolate of bursaphelenchus xylophilus. | bursaphelenchus xylophilus isolate mpsy-1av was subcultured from pathotype mpsy-1. mpsy-1av is nonparasitic and does not establish in pinus sylvestris, p. strobus, p. nigra, or p. taeda. this isolate produces ethanol as an end product of carbohydrate metabolism, whereas its parent pathotype, mpsy-1, does not. alcohol dehydrogenase activity was easily detectable in homogenates of mpsy-1av but barely detectable in some homogenates of mpsy-1. genomic differences were seen between mpsy-1 and m psy-1 ... | 1987 | 19290148 |
| genomic differences among pathotypes of bursaphelenchus xylophilus. | total genomic dna from bursaphelenchus xylophilus pathotypes mpsy-1 and vpst-1 and from b. mucronatus was digested with restriction endonucleases. dna fragments were electrophoretically separated, southern blotted to nitrocellulose, and hybridized to genomic dna from one of the isolates. the resulting hybridization patterns indicate genomic differences in repetitive dna sequences among these populations. greatest differences were seen between b. xylophilus and b. mucronatus, but genomic differen ... | 1988 | 19290214 |
| [dispersion of the nematodes belonging to the bursaphelenchus xylophilus species group with saw timber in russian federation]. | four new records of bursaphelenchus mucronatus in saw-timbers from the asian part of russia (irkutsk oblast and krasnoyarsk krai, larch, pine and spruce wood) intercepted in st. petersburg, are described, measured and illustrated. the diagnosis of b. mucronatus is amended and its relationships within species group xylophilus, and especially its differences from a quarantine pests b. xylophilus, are given. the record of the b. mucronatus transition from asiatic into european part of russia sugges ... | 2009 | 20198962 |
| structural and functional characterization of a novel, host penetration-related pectate lyase from the potato cyst nematode globodera rostochiensis. | summary the cell wall, a strong extraprotoplasmic layer surrounding plant cells that mainly consists of a variety of polysaccharides, constitutes a major barrier for potential parasites. plant-parasitic nematodes are well equipped to overcome this barrier as they produce and secrete cell-wall-degrading enzymes. expression profiling of various life stages of the potato cyst nematode globodera rostochiensis revealed a novel pectate lyase gene (gr-pel2, 759 bp). the gr-pel2 protein showed highest s ... | 2007 | 20507500 |
| composition of bacterial communities associated with a plant-parasitic nematode bursaphelenchus mucronatus. | bursaphelenchus mucronatus is a plant-parasitic nematode widely existing in eurasian pine forests. to analyze the diversity and role of bacteria associated with the nematode, culture-dependent and culture-independent methods were used to identify and characterize the composition of bacterial community. a total of 13 bacterial isolates were obtained from b. mucronatus by the culture-dependent method. sixty-four species of bacteria were identified from two 16s rdna clone libraries constructed from ... | 2011 | 20524117 |
| pathogenic roles of the bacteria carried by bursaphelenchus mucronatus. | fifty strains of bacteria were isolated from six isolates of the nematode bursaphelenchus mucronatus (bm) from china and russia and identified using the biomerieux vitek 32 system. in bioassay, 3 bacterial strains showed the high levels of phytotoxin production while 19, 16, and 12 strains showed moderately, low and no phytotoxin production, respectively. inoculation of 2-month-old pinus thunbergii seedling with each of the six bm isolates showed that the mean number of days from inoculation to ... | 2009 | 22661772 |
| bursaphelenchus xylophilus and b. mucronatus secretomes: a comparative proteomic analysis. | the pinewood nematode, bursaphelenchus xylophilus, recognized as a worldwide major forest pest, is a migratory endoparasitic nematode with capacity to feed on pine tissues and also on fungi colonizing the trees. bursaphelenchus mucronatus, the closest related species, differs from b. xylophilus on its pathogenicity, making this nematode a good candidate for comparative analyses. secretome profiles of b. xylophilus and b. mucronatus were obtained and proteomic differences were evaluated by quanti ... | 2016 | 27941947 |
| identifying virulence-associated genes using transcriptomic and proteomic association analyses of the plant parasitic nematode bursaphelenchus mucronatus. | bursaphelenchus mucronatus (b. mucronatus) isolates that originate from different regions may vary in their virulence, but their virulence-associated genes and proteins are poorly understood. thus, we conducted an integrated study coupling rna-seq and isobaric tags for relative and absolute quantitation (itraq) to analyse transcriptomic and proteomic data of highly and weakly virulent b. mucronatus isolates during the pathogenic processes. approximately 40,000 annotated unigenes and 5000 protein ... | 2016 | 27618012 |
| migration and attacking ability of bursaphelenchus mucronatus in pinus thunbergii stem cuttings. | to understand how bursaphelenchus xylophilus kills pine trees, the differences between the effects of b. xylophilus and b. mucronatus on pine trees are usually compared. in this study, the migration and attacking ability of a non-pathogenic b. mucronatus in pinus thunbergii were investigated. the distribution of b. mucronatus and the number of dead epithelial cells resulting from inoculation were compared with those of the pathogenic b. xylophilus. although b. mucronatus is non-pathogenic in pin ... | 2016 | 27493609 |
| the use of real-time polymerase chain reaction with high resolution melting (real-time pcr-hrm) analysis for the detection and discrimination of nematodes bursaphelenchus xylophilus and bursaphelenchus mucronatus. | the real-time pcr-hrm analysis was developed for the detection and discrimination of the quarantine nematode bursaphelenchus xylophilus and bursaphelenchus mucronatus. a set of primers was designed to target the its region of rdna. the results have demonstrated that this analysis is a valuable tool for differentiation of these both species. | 2016 | 26880540 |
| bacterial diversity and community structure in the pine wood nematode bursaphelenchus xylophilus and b. mucronatus with different virulence by high-throughput sequencing of the 16s rdna. | bursaphelenchus xylophilus is the pathogen of pine wilt disease. bursaphelenchus mucronatus is similar to b. xylophilus in morphology. both species share a common niche, but they are quite different in pathogenicity. presently, the role of bacteria in pine wilt disease development has been widely speculated. the diversity of bacteria associated with b. xylophilus and b. mucronatus with different virulence remains unclear. in this study, virulence of four b. xylophilus and four b. mucronatus stra ... | 2015 | 26372013 |
| nematicidal metabolites from roots of stellera chamaejasme against bursaphelenchus xylophilus and bursaphelenchus mucronatus. | bursaphelenchus xylophilus, the causal agent of pine wilt disease, severely damages pine forests, and bursaphelenchus mucronatus, a species related to b. xylophilus, is weakly pathogenic. because the use of synthetic pesticides to control pine wilt disease has raised concerns for human and environmental health, a search for useful botanically based compounds is needed. | 2014 | 23934879 |
| comparison of complete mitochondrial genomes of pine wilt nematode bursaphelenchus xylophilus and bursaphelenchus mucronatus (nematoda: aphelenchoidea) and development of a molecular tool for species identification. | we determined the complete mitochondrial genome sequences for bursaphelenchus mucronatus, one species of pinewood nematode. the genome is a circular-dna molecule of 14,583 bp (195 bp smaller than its congener bursaphelenchus xylophilus) and contains 12 protein-coding genes (lacking atp8), 22 trna genes, and 2 rrna genes encoded in the same direction, consistent with most other nematodes. based on sequence comparison of mtdna genomes, we developed a pcr-based molecular assay to differentiate b. x ... | 2013 | 23434520 |
| new insights into the phylogeny and worldwide dispersion of two closely related nematode species, bursaphelenchus xylophilus and bursaphelenchus mucronatus. | the pinewood nematode, bursaphelenchus xylophilus, is one of the greatest threats to coniferous forests worldwide, causing severe ecological damage and economic loss. the biology of b. xylophilus is similar to that of its closest relative, b. mucronatus, as both species share food resources and insect vectors, and have very similar morphological characteristics, although little pathogenicity to conifers has been associated with b. mucronatus. using both nuclear and mitochondrial dna markers, we ... | 2013 | 23409167 |
| rapid diagnosis of the infection of pine tree with pine wood nematode (bursaphelenchus xylophilus) by use of host-tree volatiles. | attraction of the bursaphelenchus xylophilus nematode toward 18 volatiles of pinus species was evaluated by a petri-dish bioassay under laboratory conditions to develop a rapid diagnostic kit. among these compounds, α-pinene, β-pinene, and camphor showed significantly higher attractiveness to b. xylophilus in both the reproductive and dispersal stages, whereas these compounds were not active against bursaphelenchus mucronatus . a trap tube was developed as a diagnostic kit, which consisted of a ... | 2012 | 22725097 |
| comparative transcriptomics of two pathogenic pinewood nematodes yields insights into parasitic adaptation to life on pine hosts. | bursaphelenchus xylophilus and bursaphelenchus mucronatus are migratory endoparasitic nematodes that live in pine trees. to gain insight into their molecular similarities and differences, transcriptomes of the two nematodes were analysed. a total of 23,765 and 21,782 contigs (>300 bp) were obtained from b. xylophilus and b. mucronatus, respectively. more than 80% of the contigs could map to each other's transcriptome reciprocally. a total of 23,467 and 21,370 open reading frames were predicted, ... | 2012 | 22705985 |
| [life-cycle of bursaphelenchus mucronatus mamiya et enda, 1979 (nematoda: aphelenchida)]. | postembryonic development of bursaphelenchus mucronatus has been studied. first molt takes place within the egg-shell, and three subsequent molts after hatching. structure of all juvenile stages are described, as well as molting juveniles and adults. juvenile stages differ in genital primordium structure, copulatory structures primordial and the body length. in juveniles of 3rd and 4th stage the sex of juvenile was distinguished: male juveniles have cloacal primordium, whereas female juveniles h ... | 2009 | 19637771 |