| effect of ascaris extract applied intravenously, on segment bronchus and the influence of ipsilateral vagus blockade. | respiratory hypersensitivity and systemic effects after local application of ascaris suum extract on the segmental bronchus and after its intravenous infusion are studied on four boxer dogs. influence of ipsilateral vagus blockade on the respiratory hyperreactivity after intrabronchial application of ascaris was also investigated on the same dogs. ascaris extract was applied in liquid form directly through a catheter into the right segmental bronchus. egg albumin was applied in the same way for ... | 1976 | 7007 |
| pyridine nucleotide transhydrogenases of parasitic helminths. | | 1976 | 8009 |
| the cat lung strip as an in vitro preparation of peripheral airways: a comparison of beta-adrenoceptor agonists, autacoids and anaphylactic challenge on the lung strip and trachea. | 1 a new in vitro preparation, the isolated lung strip of the cat, is described for investigating the direct effect of drugs on the smooth muscle of the peripheral airways of the lung. the preparation comprises a thin strip of lung parenchyma which can be mounted in a conventional organ bath for isometric tension recording. its pharmacological responses have been characterized and compared with the isolated tracheal preparation of the cat. 2 the lung strip exhibited an intrinsic tone which was re ... | 1976 | 10035 |
| proteinase activity in ascaris suum eggs, hatching fluid, and excretions-secretions. | hatching fluid and the excretions and secretions (e.s.) of hatched larvae of ascaris suum revealed proteinase activity when assayed by 2 different procedures employing collagen or casein as substrates. both assays apparently detected similar levels of proteinase activity in hatching fluid and e.s. of hatched larvae. the anson (casein substrate) assay worked best in 0.05 m phosphate buffer, ph 8.0. the azocoll (collagen substrate) assay worked best in 0.05 m borate buffer at ph 8.8. azocoll assay ... | 1976 | 10365 |
| the rotation aeration system (system fuchs) for the treatment of liquid animal and municipal wastes. 5. destruction of ascaris eggs in swine slurry and municipal sewage. | | 1976 | 10881 |
| post-proline cleaving enzyme. purification of this endopeptidase by affinity chromatography. | the endopeptidase, post-proline cleaving enzyme, has been purified 10,500-fold in an overall yield of 18% from lamb kidney. the enzyme possesses a specific activity of 45 mumol/mg/min as tested with the substrate z-gly-pro-leu-gly (km = 6.0 x 10(-5)), has a molecular weight of 115,000, is comprised of two subunits with a molecular weight of 57,000, and exhibits maximal activity at ph 7.5 to 8.0. with the exception of the -pro-pro linkage, the -pro-x-peptide bond (x equals l- and d-amino acid res ... | 1976 | 12173 |
| rapid, shallow breathing after ascaris suum antigen inhalation: role of vagus nerves. | in five treadmill-exercising, unsedated dogs, we studied the effect of inhaled ascaris suum antigen aerosols on minute volume of ventilation (ve), respiratory frequency (f), tidal volume (vt), total pulmonary resistance (rl), and dynamic pulmonary compliance (cldyn), before and during cooling of the vagus nerves. with the vagi warm, inhaled antigen increased ve (mean + 62%; p less than 0.01)by increasing f (mean + 180%; p less than 0.01), despite a decrease in vt (mean - 42%; p less than 0.01). ... | 1977 | 13058 |
| human intestinal helminthiases in east timor. | in a study of 210 people from all age groups in the venilale district of east timor, 49% had ascaris lumbricoides, 1% trichuris trichiura and 67% hookworm infection. there were high ascaris infection rates among some of the children, but the trichuris and hookworm rates were almost uniformly low. the factors responsible for these rates are enumerated, and the complex interaction of the factors is discussed. the relative lack of shade and the well-drained limestone soil are probably in large meas ... | 1976 | 16349 |
| haemagglutination tests in the study of ascaris epidemiology. | haemagglutination tests using adult ascaris suum antigen were performed on sera from 810 people from three areas in papua new guinea and east timor. in the area with the highest childhood ascaris infection rates and egg-counts (kaul), titres were highest and remained high throughout life. there was a significant negative correlation between titre and eosinophil count, and it is suggested that high titres in adult life result from continuous usually unsuccessful reinfection, and that the declinin ... | 1977 | 17370 |
| the metabolism of niclosamide and related compounds by moniezia expansa, ascaris lumbricoides var suum, and mouse- and sheep-liver enzymes. | 1. niclosamide and related nitro compounds were reduced to the corresponding amines by moniezia expansa, ascaris lumbricoides var suum and by enzymes prepared from these species and by mouse and sheep liver homogenates. the reduction of niclosamide by the helminths required as cofactors nadh2 and glutathione, but was inhibited 50% by 2 x 10(-7) m allopurinol. 2. unlike benzanilide, niclosamide was not hydrolysed by either the helminths, or the mammalian liver preparations. rates of hydrolysis of ... | 1977 | 19884 |
| involvement of histamine h1- and h2-receptors in induced asthmas in dogs. | participation of histamine h1- and h2-receptors in both asthmas, i.e. experimentally induced bronchoconstriction and bronchosecretion, with ascaris suum and histamine in anesthetized dogs was investigated. dogs given 0.2% histamine solution or ascaris antigen (3 mg protein) by inhalation showed increases in respiratory resistance (rrs) and respiratory rate to 2.5-5.0 fold. airway secretion volume was also significantly increased 3-4 fold. the increase in rrs by histamine inhalation was effective ... | 1977 | 24777 |
| investigation of the role of histamine in antigen-induced bronchoconstriction in the ascaris-hypersensitive dog. | 1 aerosol administration of ascaris antigen to the airways of ascaris-hypersensitive dogs provoked increases in pulmonary resistance (rp) and decreases in dynamic lung compliance (cdyn). these changes in pulmonary mechanics were not inhibited by the histamine h1-receptor antagonists, diphenhydramine or mepyramine. 2 increases in rp and decreases in cdyn induced by a histamine aerosol were markedly or totally inhibited by comparable doses of these h1-antihistamines. 3 doses of antigen which produ ... | 1978 | 26444 |
| isolation and partial characterization of trehalase from ascaris muscle. | the tissues of female ascaris suum were assayed for alpha,apha'-glucoside 1-d-glucohydrolase (trehalase) activity. a soluble from of the enzyme was isolated from muscle tissue and purified approximately 37-fold. the enzyme was specific for trehalose as substrate. the ph optimum for enzymatic activity was found to be 6.0, and the apparent km for trehalose was estimated to be 2.1 x 10-4 m. the product of the reaction was identified as d-glucose by chemical, chromatographic and enzymatic methods. | 1978 | 28390 |
| pyruvate and citrate metabolism in the muscle tissue of ascaris lumbricoides. | aconitase and nad linked isocitrate dehydrogenase were present in ascaris lumbricoides muscle at only very low activities, whilst there were significant levels of citrate synthase, nadp linked isocitrate dehydrogenase, 2-oxoglutarate dehydrogenase and succinic thiokinase. pyruvate dehydrogenase was present in a. lumbricoides muscle at levels comparable with mammalian tissues and results suggest that it is modulated via a phosphotransferase/phosphatase system. the tricarboxylic acid cycle interme ... | 1978 | 29388 |
| the destruction of pig helminth ova and larvae in a slurry treatment process. | it was established that a thermophilic system for the treatment of pig slurry at 55 degrees c rapidly killed the free living stages of three common pig parasites. this treatment could be beneficially incorporated in any pig slurry recycling process, whether to land or to animals. | 1978 | 33482 |
| biochemical studies on the muscle microsomes of ascaris lumbricoides var. suum. ii. purification and characterization of b-type cytochrome and nadh-ferricyanide reductase from ascaris muscle microsomes. | a b-type cytochrome and nadh-ferricyanide (fc) reductase were solubilized from ascaris muscle microsomes by detergents and purified by column chromatography. the purified b-type cytochrome displayed absorption bands at 560 (alpha-peak), 525 (beta-peak), and 424 nm (gamma-peak), with a marked shoulder at 555 nm in the reduced from, 415 nm (gamma-peak) in the oxidized form. this absorption spectrum was different from that of rat liver microsomal cytochrome b5. the molecular weight was estimated to ... | 1979 | 33974 |
| effect of levamisol on tissues of ascaris lumbricoides. | levamisol which is highly effective against ascaris infections, was shown to produce degenerative changes in the intestine and the reproductive system of the worm. worms that were not expelled by treatment produced only nonviable eggs which could not develop in vitro. levamisol is thus shown to be not only a highly curative drug, but a compound of significant epidemiological importance. | 1979 | 35937 |
| airway sensitivity to slow-reacting substance of anaphylaxis, histamine, and antigen in ascaris sensitive monkeys. | the effects of ascaris suum antigen, histamine, and slow-reacting substance of anaphylaxis (srs-a) on the respiratory system were compared in 3 anesthetized rhesus monkeys. the agents were administered by instillation into the trachea, and the animals were studied in a volume displacement body plethysmograph. two of the animals showed skin and bronchial sensitivity to ascaris suum antigen and responded to it with increased pulmonary resistance and decreased dynamic compliance. a similar response ... | 1979 | 36017 |
| ascaris suum: electrophoretic characterization of reproductive tract and perienteric fluid polypeptides, and effects of seminal and uterine fluids on spermiogenesis. | | 1979 | 36284 |
| in vitro activation and behavior of the ameboid sperm of ascaris suum (nematoda). | a system is described for the study of activation and motility of ascaris spermatozoa in vitro. activation was accomplished by addition of the sperm-activating substances (sas), extracted from the male accessory gland, to cells incubated in phosphate-buffered saline (ph 7.4) at 37-39 degrees c under anaerobic conditions (95% n2, 5% co2). activation is characterized by a change from spherical to ameboid shape with coalescence of the refringent granules. the normal ameboid spermatozoa bear several ... | 1979 | 39678 |
| isolation and some properties of an acid protease from fasciola hepatica. | a new protease, detected in an extract of fasciola hepatica, was isolated and partly purified. the ph optimum for the cleavage of denaturated haemoglobin by the enzyme is ph 3.0. this proteolytic activity is inhibited by diazoacetylnorleucine methyl ester, pepstatin, the pepsin inhibitor from ascaris suum, and phenylalanine. the cathepsin d inhibitor from potatoes, edta, mercaptoethanol and the inorganic salts tested have no inhibitory effect. the cleavage of the b-chain of oxidized insulin by e ... | 1979 | 44401 |
| histamine release during antigen inhalation in experimental asthma in dogs. | histamine release after antigen inhalation was studied in 12 dogs sensitive to nematode antigens. in 19 experiments, histamine was detected in arterial plasma after antigen inhalation; its concentration was correlated with changes in airflow resistance of the respiratory system (rrs) (per cent change above control equals 132.3 plus 250 log histamine concentration). histamine was released from the lung and was not attributable to the concomitant hypoxia. in 6 dogs given aerosols of dilute antigen ... | 1975 | 46138 |
| half-lives of two types of rat homocytotropic antibodies in circulation and in the skin. | the half-lives of two classes of rat immunoglobulins with homocytotropic properties, i.e., ige and igg2a, in the circulating blood and in the skin were studied. the catabolism of both normal (reaginic) and pathological (myeloma) ige proteins in circulation was found to be extremely rapid with a half-life of 12 h. in contrast, the half-life of ige antibody in the homologous skin was calculated to be 7.4 days. on the other hand, the half-life of igg2a in circulation was about 5 days regardless of ... | 1975 | 46847 |
| induction of mouse homocytotropic antibodies to timothy pollen antigens. | the igg1 and ige homocytotropic antibody responses of laf and c3h mice to timothy pollen antigens are defined. both mouse strains responded to low doses of crude timothy pollen extract (wst) or a major antigen of timothy pollen coupled to a purified fraction of ascaris suum (antigen b-ascaris). titers in laf mice were greater than those in c3h mice. regardless of the immunogen, antigen b was the major determinant recognized by the homocytotropic antibodies; pca titers with wst or antigen b for c ... | 1975 | 50362 |
| specificity and sensitivity of skin test reactions to extracts of toxocara canis and ascaris suum ii. homologous 48-hour passive cutaneous anaphylaxis tests with sera from infected guinea pigs. | the specificity and sensitivity of adult and larval somatic antigens and perienteric fluid of toxocara canis and ascaris suum were investigated by using a modified passive cutaneous anaphylaxis procedure in guinea pigs. pooled sera from animals infected with low doses (0.01, 0.1, or 1.0 egg/g) were most reactive with the homologous larval antigen preparation. however, the toxocara antisera were highly reactive with this antigen only, whereas the ascaris antisera reactions could not be interprete ... | 1975 | 50748 |
| angiostrongylus cantonensis: preparation of a specific antigen using immunoadsorbent columns. | | 1975 | 51799 |
| heterologous reactions involving parasites, blood group antibodies and tissue components. | the sera of many patients with malaria and filariasis, and also anti-a and b blood group sera, were found to react by immunofluorescence with the somatic musculature of nematodes, especially ascaris, and human and rat muscle, especially the skeletal type. these reactions were attributed to a polysaccharide related to ab substance in adult nematodes and to raised ab antibodies in malaria. similar heterologous reactions were found to involve the integument of schistosome adult worms and the microf ... | 1975 | 52213 |
| the sites of production and localization of ige in canine tissues. | | 1975 | 52320 |
| reagin mediated hypersensitivity: the ascaris model. | | 1975 | 52602 |
| the effect of flavins and enzyme inhibitors on 4-nitrobenzoic acid reductase and azo reductase of ascaris lumbricoides var suum. | 1. the effects of inhibitors and activators on the azo- and nitro-reductases of ascaris lumbricoides var suum have been investigated. both types of reduction were inhibited by fad, fmn, riboflavin, allopurinol, dicoumarol, 5-nitro-2-furaldehyde, azide and cyanide at concentrations of 1 mm. neither reaction was inhibited by menadione, nitrofurantoin, skf 525-a or fluoride. both reactions were stimulated by addition of hypoxanthine. 2. the enzyme preparation contained no detectable aldehyde oxidas ... | 1975 | 52946 |
| a rapid whole-mount staining procedure for ascaris embryo chromosomes. | | 1975 | 54958 |
| potentiation of ige-mediated cutaneous reactivity and blood leucocyte histamine release by deuterium oxide. | a previous study (gillespie & lichtenstein, 1972) demonstrated that there was potentiation of histamine release from human peripheral blood leucocytes following exposure to antigin or anti-ige in deuterium oxide (d2o). the current study confirms the results with human leucocytes and indicates that the degree of histamine release due to anti-ige or its potentiation by d2o appeared independent of the serum ige concentration of the cell donor. further studies demonstrated that the peripheral blood ... | 1975 | 55323 |
| reagin-mediated asthma in rhesus monkeys and relation to bronchial cell histamine release and airway reactivity to carbocholine. | rhesus monkeys with persistent immediate-type cutaneous and respiratory responses (rr) to ascaris antigen (aa) were compared with rhesus monkeys with skin reactivity and no respiratory responses, and animals with no skin reactivity and no respiratory responses to inhaled antigen (nr). the rr group could not be distinguished from the nonresponding (nr) group by the cutaneous skin test titers, serum, or respiratory secretion ige concentration. leukocyte histamine (h) release due to anti-ige was s ... | 1976 | 55417 |
| [pathogenesis of larval ascariasis]. | | 1975 | 55954 |
| ige-mediated histamine and srs-a release from respiratory cells and peripheral blood leukocytes of rhesus monkeys. | peripheral blood leukocytes (pbl) and free respiratory cells (rc) of the mast cell-basophil type were obtained from normal rhesus monkeys or rhesus monkeys with defined immediate-type hypersensitivity to ascaris antigen (aa). pbl or rc from the latter were exposed to aa and the former were exposed to anti-ige. histamine (h) release and release of a slow reacting substance of anaphylaxis (srs-a) occurred following exposure of the appropriate cells to aa or anti-ige. the release of both h and srs- ... | 1976 | 58954 |
| parasites and asthma in tanzanian children. | it has been claimed that parasitic infestation suppresses the development of asthma. eight tanzanian schoolchildren in whom asthma was confirmed by the demonstration of exercise-induced bronchospasm were found in an ascaris-endemic area of rural tanzania. faecal parasites were found equally commonly in the children with asthma and the ninety-seven non-asthmatic controls. the prevalence of asthma (3.3%) in the school under study was comparable with that found in developed countries. | 1976 | 61392 |
| lymphocytes and specificity of eosinophilia. | repeated injections of soluble antigen derived from the body fluid of ascaris lumbricoides induced a substantial accumulation of eosinophils in the peritoneal fluid of mice. evidence of an underlying immune mechanism was sought. a single injection of antigen was ineffective in eliciting eosinophilia, but it primed recipients to respond to a subsequent injection 30 days later. furthermore, this priming effect could be transferred with lymphoid cells. depletion of t cells by adult thymectomy and i ... | 1976 | 61627 |
| cytophotometric study of nuclear proteins during gametogenesis in ascaris lumbricoides. | | 1976 | 61882 |
| an in vitro model of canine immediate-type hypersensitivity reactions. | fragmented lung prepared from dogs cutaneously sensitive to ascaris antigen released histamine and a slow-reacting substance of anaphylaxis-like (srs-a) material upon antigen challenge. passive sensitization of fragments with serum obtained from ascaris-sensitive donor dogs enhanced the release of both substances; heating (56 degrees c for 4 h) destroyed the ability of the serum to enhance release. in passively sensitized tissues isoproterenol and papaverine inhibited the release of histamine an ... | 1976 | 63451 |
| the specificity of nematode allergens in the diagnosis of human visceral larva migrans. | extracts of nematodes have been used as skin test antigens in the diagnosis of nematode infections for many years. ascaris lumbricoides and toxocara canis are two nematodes commonly involved in human parasitism, the latter being associated with the clinical condition of visceral larva migrans. in vitro and in vivo experiments reported in this paper confirm experimentally, as well as clinically, the existence of cross-reacting antigens between t. canis and ascaris spp., and probably between t. ca ... | 1976 | 65168 |
| effect of periodic deworming on nutritional status of ascaris-infested preschool children receiving supplementary food. | the weight for age of 154 undernourished preschool children in an ascaris-infested community receiving supplementary food was monitored for a year. 74 children were given tetramisole every 4 months, while 80 controls were given placebo. the prevalence of ascaris (roundworm) infection and the severity of protein-energy malnutrition were the same in the two groups at the onset of the study. stool-positive rates for ascariasis came down significantly in the experimental group during the year, but e ... | 1977 | 69194 |
| pharmacologic profile of a new antiallergic compound prd-92-ea. | prd-92-ea [5,5-dimethyl-11-oxo-5h, 11h-(2) benzopyrano (4,3-g) (1) benzopyran-9-carboxylic acid ethanolamine], was an active antiallergic compound in rat and monkey experimental models of immediate hypersensitivity. it inhibited, in a dose-dependent manner, the rat pca reaction after both intravenous and oral administration. it also inhibited the degranulation of rat peritoneal mast cells after antigenic challenge. prd-92-ea was also active in preventing bronchoconstriction in ascaris-sensitive ... | 1977 | 73514 |
| ige-type antibodies to ascaris antigens in man. | ige antibodies to ascaris antigens were detected by radioallergosorbent test (rast) in the serum obtained from a person experimentally sensitized with ascaris suum antigens prepared from the body fluid of worms. this serum passively sensitized human skin in vitro and histamine release from the sensitized skin was observed by challenge with antigens. the allergenicity of a. suum antigens was present over a very broad spectrum of molecular size. the present results suggest that specific ige antibo ... | 1977 | 73520 |
| pharmacologic regulation of antigen-induced mediator release from canine lung. | the ascaris antigen-induced release of histamine and a slow-reacting substance of anaphylaxis (srs-a) from passively sensitized fragmented canine lung is further characterized. histamine and srs-a were released within 30 sec of antigen challenge, reached a maximum at 7 and 10 min, respectively, and thereafter appeared to remain constant to 30 min. contractions of guinea pig ileum produced by canine srs-a were competitively antagonized by the srs-a antagonist fpl-55712. indomethacin and deuterium ... | 1978 | 73521 |
| the effect of catecholamines on the in vivo and in vitro responses of the cat lung during anaphylaxis. | 1. anaphylaxis in the lung of cats actively sensitized to ascaris antigen has been investigated in vivo and in vitro. 2. in vivo there was a 100% increase in airways resistance and a 50% decrease in dynamic lung compliance following intravenous challenge with ascaris antigen. prostaglandin f2alpha induced similar changes but with histamine only dynamic lung compliance was affected. (-)-isoprenaline prevented these prostaglandin f2alpha- and histamine-induced changes and caused a delay of about 2 ... | 1977 | 74265 |
| antigen-, anti-f(ab')2- and anti-ige-induced histamine release from rat mast cells. | the existence of ige antibody molecules on peritoneal mast cells from rats immunized with dinitrophenylated ascaris extract was demonstrated by double antibody immunofluorescence staining and by histamine release induced by anti-ige antibodies. cross-linking of the ige molecules, which are fixed to mast cells, by interaction with either the homologous antigen or with antibodies to the fab or fc regions of ige, triggered in the presence of calcium ions a chain of intracellular reactions which inv ... | 1978 | 74366 |
| purification and characterisation of two proteins from ascaris suum extract, antigenically different but bearing common allergenic epitopes. | two proteins, fi and fvii, have been purified from a whole worm extract of adult ascaris suum, using ammonium sulfate precipitation, sephadex gel filtration and deae cellulose chromatography. both of them provoke reagin formation in mice as tested in presensitized animals with an original immediate hypersensitivity reaction test. they are two chemically and physically different protein molecules showing no cross reaction with a rabbit serum anti-crude extract but bearing common allergenic epitop ... | 1978 | 78891 |
| serological tests for toxocara. | | 1978 | 79948 |
| the in vivo transfer of antigen-induced airway reactions by bronchial lumen cells. | rhesus monkeys with airway responses to aerosol challenge with ascaris antigen constitute a primate model of inhalant asthma. previous studies have shown that bronchial lavage cells from airway-reactive animals will release histamine or a slow-reactive substance of anaphylaxis after challenge with antigen. because these bronchial lumen cells are the first cells in contact with inhaled antigen, they may play a role in induction of antigen-induced airway responses. to evaluate this possibility, br ... | 1978 | 80408 |
| radioallergosorbent and indirect fluorescent antibody tests in immunodiagnosis of schistosomiasis. | radioallergosorbent tests for specific ige antibodies to schistosoma haematobium and s. mansoni were positive in 82 and 72%, respectively, of sera from 136 african schistosomiasis patients. 99% of sera from controls (5 free of parasitic disease, 75 infested with hookworm, ascaris, trichuris, and/or onchocerca) were negative. when titres in indirect fluorescent antibody tests were set at the same level of specificity only 38% of patients' sera were positive. a cut-off at a higher concentration in ... | 1978 | 82737 |
| [helminth stimulation and suppression of host immune reactions to heterologous antigens. ii. the immunosuppressive action of helminths]. | | 1978 | 82906 |
| pharmacologic characterization of the antiallergic activity of sk&f 78729-a. | sk&f 78729-a, one of a series of pyranenamine compounds, was studied in several laboratory models of immediate-type hypersensitivity reactions. in vivo, sk&f 78729-a, i.v./p.o., inhibited the antigen-induced rat 48-hr passive cutaneous anaphylaxis by a mechanism other than end-organ antagonism to either histamine or serotonin. in vitro, the compound produced a concentration-related inhibition of the immunologic release of histamine and slow-reacting substance of anaphylaxis from passively sensit ... | 1978 | 83359 |
| regulation of ige antibody production by serum molecules. v. evidence that coincidental sensitization and imbalance in the normal damping mechanism results in "allergic breakthrough". | experiments presented in this paper were designed to test a new concept concerning the possible pathogenesis of the allergic phenotype. this concept, termed "allergic breakthrough" considers that one of the avenues toward the allergic phenotype involves coincidental sensitization combined with an imbalance in the normal damping mechanism that serves to limit ige antibody production. the three predictions of this concept that can be tested experimentally are: 1) manipulations that are effective i ... | 1979 | 87455 |
| ige antibody formation and aging. i. age-related changes in ige antibody formation and avidity for the dnp-determinant in mice. | | 1979 | 87474 |
| [fractionation of the trichinella spiralis antigen and allergy diagnosis trails]. | experiments were carried out to fractionate an antigen of trichinella spiralis with sephadex g-50 and g-100. the specificty of the fractions has been followed up by means of the intradermal allergic reaction in six pigs, experimentally infected with trichenalla spiralis, one with trichocephalus suis and three, born by trichinellosis-affected sows. the second fraction has been tested also on four pigs, experimentally inoculated with ascaris suum, nine--with ascarsis suum and oesophagostomum and 1 ... | 1978 | 88790 |
| the pathophysiology of asthma. | because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ... | 1979 | 89006 |
| conscious primate model for evaluating antiallergic drugs. | | 1979 | 91960 |
| comparative evaluation of 7 helminth antigens in the enzyme-linked immunosorbent assay (e.l.i.s.a.). | 112 sera from europeans with parasitologically proven helminthiasis were tested in the enzyme-linked immunosorbent assay (e.l.i.s.a.) against 6 crude extracts of various helminths (2 of adult worms: dipetalonema viteae, fasciola hepatica; 3 of eggs: ascaris suum, toxocara canis, schistosoma mansoni; and of echinococcus granulosus scolices) and against bovine hydatid fluid. each serum was tested simultaneously at a fixed dilution of 1:160 against all antigens. extensive cross-reactions were obser ... | 1979 | 92420 |
| [the presence of antioxidative defense enzymes in the ascarid ascaris lumbricoides]. | superoxide dismutase, glutathione peroxidase and glutathione have been discovered in muscles of the ringworm a. lumbricoides. superoxide dismutase activity was also revealed in eggs and larvae. using polyacrylamide gel electrophoresis and electrofocusing, it was shown that this enzyme exists in ringworm muscles in multiple molecular forms. oxygen poisoning of the ringworm and aerobic incubation of muscle homogenates in the presence of fe+2 do not result in accumulation of malonyldialdehyde. bioc ... | 1979 | 95848 |
| [effectiveness of antigens from various developmental stages of ascaris suum in the deomonstration of the migratory phase of experimental ascariasis in pigs]. | tests of the efficiency of antigens prepared from different developmental stages of ascaris suum in indirect haemagglutination test in the course of proving the migration phase of experimental ascariasis in pigs show that the antigens prepared by ultrasound from the invasive larval stage of a. suum in comparison with antigens of sexually mature stages have higher serological activity. by using this antigen it is possible to prove specific antibodies in experimentally invaded pigs from 6 to 120 d ... | 1978 | 96575 |
| [comparison of the sensitivity of the passive, indirect hemagglutination reaction and the latex fixation test in the demonstration of the migratory phase of experimental ascariasis (ascaris suum) in swine]. | the authoresses verified diagnostic sensitivity of two serological tests -passive indirect hemagglutination [iha] reaction modified by the authoresses and latex-fixation test [lft] when proving the post-invasion migratory phase of ascariasis [ascaris suum] in experimentally infected swine. the iha reaction was found to be more sensitive than the lft in most cases [62.2%] by two to three values of the geometric series. the invasion can be detected by the iha reaction from 7th to 120th day and by ... | 1978 | 97840 |
| aerosolized antigen dose-response studies in asthmatic monkeys. | this study used rhesus monkeys which had consistent respiratory responses to a standard (single dose) aerosolized concentration of a. beginning at lower concentrations of a which did not produce an airway response, fourfold increases in concentrations of a were aerosolized until an airway response occurred. compared with the single-dose sac after the tac, some consistently reactive animals did not respond to the sac, the maximal concentration of a used for testing. thus the tac may result in an ... | 1978 | 98604 |
| a macrophage chemotactic factor sharing common antigenicity with immunoglobulin g from dnp-ascaris extract-induced skin lesion in guinea-pig. | a macrophage chemotactic factor (factor a) was extracted from dnp-ascaris extract-induced skin lesions (showing a maximal macrophage reaction) in guinea-pigs and then purified by gel filtration on sephadex g-100 and chromatography using deae-sephadex and cm-sephadex. this material was a thermolabile protein (free of nucleic acid) with a molecular weight of about 150,000. it shared commom antigenicity with serum igg, and its chemotactic acitivity was completely absorbed by anti-igg and anti-light ... | 1978 | 98903 |
| a study of the effect of slow-reacting substances of anaphylaxis on the rhesus monkey airway. | slow-reacting substance of anaphylaxis (srs-a) generated from rat peritoneal mast cells was aerosolized to the airways of a group of rhesus monkeys with established airway responses to ascaris antigen. a selective effect of srs-a on pulmonary resistance and a lesser but significant effect on compliance was observed which differed from antigen, histamine, carbocholine, or prostaglandin (pg) f2alpha responses. the airway recovery from the pr change is slower than that from histamine and simulated ... | 1978 | 101573 |
| airway responses to histamine and methocholine in ascaris suum-allergic rhesus monkeys. | we performed dose-response curves to inhaled histamine (h) and methacholine (mc) in a group of eight rhesus monkeys, with and without natural allergy to ascaris suum antigen (aa). the animals were anesthetized with pentobarbital sodium, 25 mg/kg im and studied in a volume-displacement body plethysmograph. the dose of h or mc producing a 50% increase in pulmonary resistance (rl) was used to determine sensitivity to these agents and the increase in rl at a given dose was employed as a measure of r ... | 1978 | 103872 |
| [heat flushes during menopause; hyperthyroidism or carcinoid?]. | | 1979 | 104157 |
| bronchial mucosal permeability. | the tracheobronchial epithelium has well-developed tight junctions which on a morphologic basis should be markedly resistant to penetration by protein molecules. despite this, antigen inhalation in monkeys allergic to ascaris suum results in the rapid onset of pulmonary physiologic changes. recent studies in man and animals have shown that a substantial number of mast cells exist in the bronchial lumen and epithelium. we suggest that antigen-antibody interaction initially occurs on these superfi ... | 1979 | 104888 |
| differentiated b lymphocytes. potential to express particular antibody variable and constant regions depends on site of lymphoid tissue and antigen load. | b cells have the potential to respond to an antigen by producing antibodies with a variety of variable and constant regions. we have quantitatively analyzed b-cell potential at the single cell level to determine the effect of lymphoid tissue site and antigen load on the expression of variable and constant regions. concerning variable region expression, although the total frequency of b-cell precursors for phosphorylcholine is similar between nonimmune spleen and gut-associated peyer's patch tiss ... | 1979 | 105075 |
| [monitoring the helmintological situation in a large-scale reproduction of pigs]. | in 1976 to 1978, the occurrence of helminths in a large reproduction herd of pigs was studied in different age categories of the animals kept. six helminth species were found in 30.7% of the sows: ascaris suum (6.6%), oesophagostomum dentatum (24.0%), hyostrongylus rubidus (3.3%), trichocephalus suis (1.3%), strongyloides ransomi (0.7%), metastrongylus sp. (0.3%). four- to seven-month-old auction gilts had only the eggs of a. suum (4% of all cases). no helminith eggs were found in fattened pigle ... | 1979 | 106504 |
| ascaris-induced respiratory responses in the conscious rhesus monkey. | reproducible immediate-type respiratory responses were evoked in conscious monkeys, sensitive to inhaled ascaris suum, for periods up to 18 months. these responses were characterized by decreases in tidal volume and increases in breathing rate that persisted for about 40 min. maximum changes were seen 3--5 min after exposure to the aerosolized antigen and were often accompanied by coughing and increased movement of the animals within the plethysmograph used for monitoring their ventilatory chang ... | 1979 | 109398 |
| [effects of mebendazole and cambendazole on enterohelminths in pigs]. | mebendazole, administered at a dose of 30 mg active substance per 1 kg of feed, was found to have 100% effectiveness on ascaris suum and cambendazole, administered at a dose of 1.5 g per 1 kg of liver weight, showed the same effectiveness in the control of ascaris suum and oesophagostomum dentatum. the effectiveness of both drugs on trichocephalus suis and strongyloides ransomi was low. mebendazole and cambendazole can be recommended for mass dehelminthization of pigs in affected stocks. helmint ... | 1979 | 111399 |
| jejunal mucosa in marasmic children. clinical, pathological, and fine structural evaluation of the effect of protein-energy malnutrition and environmental contamination. | seven children suffering from marasmus were investigated clinically, biochemically and morphologically. the fine structure of the jejunal mucosa obtained by peroral biopsy was evaluated. the mucosal changes noted agree with the only other ultrastructural study reported by brunser et al. (8) and add information on three additional features: an increase in theliolymphocytes, excessive epithelial cell extrusion and abnormalities in the appearances of the mucosal plasma cells, suggesting possible lo ... | 1979 | 111537 |
| relationship between airway hyperreactivity and hyperpermeability in ascaris-sensitive monkeys. | in four ascaris-sensitive rhesus monkeys, we measured the fractional absorption of 3h-histamine (3hh) and airway response, as pulmonary resistance (r1), to standard histamine aerosols containing tracer amounts of 3hh for control runs (run 1) and runs after ascaris antigen challenge (run 2). the mean rate of accumulation of radioactivity in the plasma volume as a function of delivered dose during histamine exposure (2 min) was fivefold greater for run 2 (0.047% delivered dose/min) as compared wit ... | 1979 | 112136 |
| the passive transfer of immediate-type airway responses to nonreactive rhesus monkeys by antigen reactive bronchial lumen mast cells. | | 1978 | 112753 |
| the effect of lodoxamide ethyl [diethyl n,n'-(2-chloro-5-cyano-m-phenylene)dioxamate] on in vivo anaphylactic reactions. | orally administered iodoxamide ethyl (u-42,718) inhibited anaphylactic reactions in a dose-related manner in the following test animals: (1) in the rat pca reaction, excellent activity (75% inhibition at 0.1 mg/kg) was seen with a duration of activity of 30 min, (2) in the ascaris-sensitive primate (45% inhibition at 1.0 mg/kg) in lung parameters related to increased resistance and decreased compliance which persisted for up to 3 h, and (3) 50 mg/kg protected guinea pigs, sensitized to egg album ... | 1979 | 115253 |
| structure and function of airways in ascaris-sensitive monkeys. | | 1979 | 116120 |
| aldose reductase and sorbitol dehydrogenase in the muscle of ascaris suum (nematoda). | the muscle of ascaris suum contains sorbitol dehydrogenase [ec 1.1.1.14] and the apparent km values indicate that the enzyme favours fructose formation. aldose reductase [ec 1.1.1.21] is also present and like the mammalian enzyme it has a very high apparent km for glucose. possible functions of the sorbitol pathway in ascaris muscle are discussed. | 1978 | 116186 |
| airway responses to histamine and methacholine in ascaris suum-allergic rhesus monkeys. | | 1979 | 118144 |
| [modern therapy of intestinal helminthiasis (author's transl)]. | the modern chemotherapy of intestinal worm diseases is essentially based on niclosamide, thiabendazole and mebendazole. thus an effective therapy with few side effects has become possible for these helminthiases. in infections with taenia, ascaris, trichuris, ankylostoma, strongyloides, trichostrongylus and oxyuris, as the most frequently imported helminths, an elimination rate of over 90% of the parasites can be attained. infections with nematode larvae as with larva migrans and trichinoses are ... | 1979 | 120505 |
| serologic differentiation of adult toxocara and ascaris. | the antigenic analysis of toxocara canis a causative agent of visceral larva migrans and ascaris suum has been carried out. agar gel double diffusion techniques and its modifications were employed to determine whether or not crude antigens, obtained from the adult parasites: (a) contained components unique for each, (b) could be used to differentiate the parasites serologically. adult toxocara canis and ascanis suum antigens used in this study were found to contain several unique antigenic compo ... | 1979 | 121695 |
| serologic differentiation of larval toxocara and ascaris. | antigenic extracts of adult and larval t. canis and a. suum have been used in microgel double diffusion technique to differentiate antibodies produced in animals, experimentally infected with these parasites. antigenic extracts from adult ascaris reacted with the sera from animals infected with a. suum but the extracts from adult toxocara failed to react or reacted feebly with the sera from animals infected with t. canis. interestingly the antigenic extract from adult toxocara reacted with the s ... | 1979 | 121696 |
| letter: ascaris larvae and ova in the core of bile-duct stones in rangoon. | | 1975 | 124966 |
| studies on the subunits of myosin from muscle layer of ascaris lumbricoides suum. | 1. a purified preparation of ascaris myosin was obtained from the muscle layer of ascaris lumbricoides suum, using gel filtration and ion-exchange chromatography. 2. ascaris myosin whether purified or unpurified, had almost the same ability for atp-splitting and superprecipitation. 3. ascaris myosin and rabbit skeletal myosin were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a significant difference in the number of light chains between both myosins was found. ascaris ... | 1975 | 127618 |
| the effects of ammonium, inorganic phosphate and potassium ions on the activity of phosphofructokinases from muscle and nervous tissues of vertebrates and invertebrates. | 1. the effect of nh4+, pi and k+ on phosphofructokinase from muscle and nervous tissues of a large number of animals was investigated. the activation of the enzyme from lobster abdominal muscle by nh4+ was increased synergistically by the presence of pi or so4(2-). in the absence of k+, nh4+ plus pi markedly activated phosphofructokinase from all tissues studied. in the presence of 100 mm-k+, nh4+ plus pi activated phosphofructokinase from nervous tissue and muscle of invertebrates and the enzym ... | 1975 | 128356 |
| [experimental studies on anthelmintics (xxvi). biochemical and pharmacological studies of 4-iodothymol on ascaris lumbricoides suum]. | we have shown previously that 4-iodothymol (it) produces a contraction in ascaris muscle, probably due to the myogenic action. in this paper, the effects of it on the carbohydrate metabolism in ascaris muscle have been investigated in comparison with those of hexylresorcinol (hex), santonin (s) and piperazine (pip). (1) hex (200 approximately 400 mug/ml) showed a strong nonspecific inhibition on the formation of succinate from fumarate in muscle homogenate, the phosphofructokinase (pfk) activity ... | 1976 | 136389 |
| [interrelationship of sanitary-helminthological and epidemiological indices in microfoci of ascaridiasis]. | | 1976 | 137169 |
| [treatment of soil with carbathione in a stable microfocus of ascariasis]. | | 1976 | 139533 |
| [seasonal dynamics of contamination of the soil with the eggs of ascaris lumbricoides in the foci of ascariasis]. | | 1976 | 140295 |
| [study of the effectiveness of the soil method of dehelmintization of waste products used as fertilizers]. | | 1977 | 144851 |
| [antiallergic asthma effect of ipatropium bromide (sch 1000) in dogs (author's transl)]. | antiasthmatic effect of ipratropium bromide (sch 1000) which is a quarternary ammonium derivative of atropine was investigated in comparison with atropine in anesthetized dogs. asthmatic responses were provoked with inhalation of ascaris suum antigen. sch 1000 (0.1 approximately 0.3%) and atropine (0.1 approximately 0.3%) were given by inhalation for 10 min prior to or after the antigen inhalation. pretreatment of sch 1000 and atropine inhibited the increase in respiratory resistance (rrs) induc ... | 1977 | 145994 |
| effect of ultraviolet radiation on the infective stages of ascaris suum and stephanurus dentatus with a comparison of the relative susceptibilities of some parasitic nematodes to ultraviolet. | | 1978 | 147934 |
| gut eczema in slaughterhouse workers. | in danish bacon factories workers eviscerating and cleansing gut casings commonly develop a vesicular eczema starting in the finger webs, from where it spreads to the sides of the fingers. the eruption is called "gut eczema" or "fat eczema" by the workers. it is self-limiting, lasts for 1--2 weeks, and can recur at intervals of months or years. the etiology is unknown. prick tests with extracts of ascaris and several organs of the pigs were negative. | 1978 | 148996 |
| [long-term survival of ascarid eggs (ascaris lumbricoides l., 1758) in the soil of samarkand]. | | 1978 | 150490 |
| effect of pyrantel tartrate and carbadox on acquisition of the swine kidneyworm (stephanurus dentatus) and other parasites by pigs on contaminated lots. | a combination of pyrantel tartrate (106 mg/kg of body weight) and carbadox (55 mg/kg of body weight) in ground feed was fed to 20 weaned pigs (av wt, 14.4 kg) for 42 days. another group of 20 pigs included nontreated controls. the pigs were farrowed and suckled in a slat-floored farrowing house and had minimal exposure to the small intestinal threadworm (stronglyoides ransomi) until they were placed on severely contaminated dirt lots at the start of the experiment. five pigs from each of the two ... | 1979 | 160763 |
| surface ultrastructure and cytochemistry of parasitic helminths. | | 1975 | 164363 |
| idiopathic acquired cold urticaria in north india. | forty-three otherwise healthy patients mostly between the ages of 10 and 40 were seen in north india during a two-year period with urticaria which developed on taking a cold water bath, exposure to cold winds, evaporation of rain water or sweat in that order of frequency. there was no familial predisposition to cold urticaria or atopic disorders. the refrigeration test for cryo-proteins was negative. only one patient developed a wheal on contact with ice but 38 of 43 patients showed an exaggerat ... | 1975 | 166579 |
| parasitology survey and seroepidemiology of amoebiasis in south kalimantan (borneo), indonesia. | a parasitology surevy was conducted among inhabitants of 7 villages in 3 regencies in south kalimantan province, indonesia. a total of 2,169 stool specimens, 2,756 blood smears and 1,027 serum specimens were obtained, representing samples from approximately 10%, 12% and 5% of the population, respectively. one to 8 different intestinal parasitic infections were detected in 97% of the people. those parasites most frequently found were trichuris trichiura (83%), ascaris lumbricoides (79%), and hook ... | 1975 | 167454 |
| a survey of intestinal parasitism in a community on the pan american highway route in eastern panama. | a survey of intestinal parasitism was conducted at yaviza, a town in eastern panama's province of darien. single stool specimens from 202 persons, representing 11 percent of the town's total population, were examined. in all, 181 (90 per cent) of the specimens examined were found positive for one or more potentially pathogenic parasites. the most common parasite found was trichuris trichiura, which was observed in 80 per cent of the samples. others were ascaris lumbricoides, in 62 per cent of th ... | 1975 | 167889 |
| 4-nitrobenzoic acid reductase of ascaris lumbricoides var suum. substrate specificity and reaction products. | 1. the substrate specificity of nitro-reductase from ascaris lumbricoides varsum was determined. this enzyme reduced nitrobenzene, 4-nitrohippuric acid and the isomers of nitrophenol, nitroanisole, nitrobenzoic acid, nitrobenzaldehyde and nitrobenzyl alcohol. the same enzyme preparation reduced azobenzene, 4-dimethylaminoazobenzene and 1,2-dimethyl-4-(4-carboxyphenylazo)-5-hydroxybenzene. nitrobenzaldehyde isomers were not reduced to the alcohols. 2. the products of nitro- and azo-reduction were ... | 1975 | 168691 |
| the distribution of the ca++-dependent protein activator of cyclic nucleotide phosphodiesterase in invertebrates. | | 1975 | 168896 |