| an epidemiological analysis of factors influencing the increase in taenia ovis prevalence during the new zealand echinococcus granulosus control program. | there was an 8-fold increase in the national prevalence of taenia ovis in dogs during the first decade of the new zealand hydatid disease control program. the temporal patterns in the prevalence of t. ovis were similar in both rural working dogs and urban pet dogs, with the highest prevalence found in the sheep farming areas. the dramatic increase in the prevalence of t. ovis was a reflection of the change in source of food for many working dogs, from the traditional raw viscera to carcase meat ... | 1977 | 303900 |
| a survey of gastro intestinal helminth parasites of stray dogs in zaria, nigeria. | a survey of helminth parasites in stray dogs was carried out in the zaria area. out of 180 dogs examined 65.6 per cent (118/180) were found positive for ancylostoma caninum, while toxocara canis and trichuris vulpis were detected in 41.1 per cent (74/180) and 13.9 per cent (25/180) respectively. other helminth parasites found taenia ovis 35 per cent (63/180) taenia hydatigena 67.8 per cent (122/180), echinococcus granulosus 0.006 per cent (1/180) and dipylidium caninum 97.8 per cent (176/180). | 1979 | 452351 |
| the passive transfer of immunity to taenia ovis in lambs via colostrum. | colostrum from ewes that had been repeatedly exposed to cestode infection, whether or not their immunity was boosted by vaccination with taenia ovis, transferred a strong immunity to the lambs. susceptible ewes, not recently exposed to tapeworm eggs, gave no protection to their lambs via the colostrum. there was no evidence that colostrum-deprived lambs were more susceptible to infection with t ovis than lambs that received colostrum from non-immune ewes. colostrum from naturally immune vaccinat ... | 1979 | 523804 |
| the duration of passive protection against taenia ovis larvae in lambs. | in an attempt to induce passive protection in lambs against taenia ovis larvae that would last for the 15-20 weeks from birth to slaughter as fat lambs, one group of ewes was immunized by a series of injections of 2000, 4000, 8000, 16 000 and 32 000 activated oncospheres of taenia ovis prior to parturition. another group of ewes was not immunized. all ewes had previously grazed pasture lightly infected with t. ovis eggs. most lambs from non-immunized ewes developed cysts after oral infection wit ... | 1979 | 537831 |
| 'arc 5' antibodies in sera of sheep infected with echinococcus granulosus, taenia hydatigena and taenia ovis. | the 'arc 5' precipitin band, formed when test human serum is reacted against immunoelectophoresed hydatid cyst fluid antigen, has provided a positive diagnosis of echinococcus granulosus infection. these antibodies to 'arc 5' antigen have now been found in the sera of sheep. they appear 2 weeks after infection with taenia ovis, after 3 weeks with t. hydatigena and after 16 weeks with e. granulosus. an antigen similar to the 'arc 5' antigen of e. granulosus cyst fluid was also demonstrated in cys ... | 1979 | 551375 |
| vaccination of lambs against taenia ovis infection using antigens collected during in vitro cultivation of larvae: passive protection via colostrum from vaccinated ewes and the duration of immunity from a single vaccination. | fifty merino-cross ewes were vaccinated approximately four weeks before parturition with antigens collected during in vitro cultivation of taenia ovis larvae. a further 50 pregnant ewes were sham-vaccinated at the same time. when the first 20 lambs from each group of ewes were one to two weeks old they were placed, with the ewes, on an experimental paddock heavily contaminated with t ovis eggs. after six weeks the lambs were killed and their total carcase musculature and hearts examined for cyst ... | 1977 | 605303 |
| the effect of niclosamide on echinococcus granulosus, taenia hydatigena and taenia ovis infections in dogs. | the effect of niclosamide (particle size 1 micron m) and its piperazine salt (particle size 5-10 micron m) on echinococcus granulosus and taenia hydatigena has been investigated. the piperazine salt of niclosamide was also tested for activity against taenia ovis. these trials involved 326 dogs. irrespective of particle size, the drug was found to be virtually inactive against 28-day-old e granulosus. some evidence was found indicating that there may be a low degree of activity against 70-day-old ... | 1977 | 877439 |
| prevalence of echinococcus granulosus and other cestodes in dogs on the navajo reservation in arizona and new mexico. | fecal specimens from 429 dogs purged with arecoline hydrobromide at 131 rural camps on the navajo reservation in arizona and new mexico were examined for cestodes. echinococcus granulosus was found in feces from 3 dogs at 3 different camps. other cestodes present were taenia hydatigena--45.5%, taenia pisiformis--21.0%, taenia serialis--11.9%, and taenia ovis--1.6%. | 1977 | 879566 |
| the effect of praziquantel on echinococcus granulosus, taenia hydatigena and taenia ovis infections in dogs. | the effect of praziquantel on echinococcus granulosus, taenia hydatigena and t ovis was investigated in a trial involving 360 dogs. the drug was found to be highly toxic to tapeworms. few dogs remained infected after a single treatment within the range of dose rates tested (0-31 mg/kg to 10-0 mg/kg body weight). no toxicity to dogs was observed. | 1977 | 905644 |
| studies on the fate of taenia hydatigena and taenia ovis larvae in rabbits, and cross immunity with taenia pisiformis larvae. | | 1977 | 924711 |
| vaccination of lambs against infection with taenia ovis using antigens collected during short-term in vitro incubation of activated t. ovis oncospheres. | activated oncospheres of taenia ovis were incubated for 24 h or 48 h at 37-5 degress c in medium 858 alone or in medium 858 enriched with 20% lamb serum or foetal calf serum. antigens contained in the supernatant medium from these incubations were concentrated, emulsified in freund's incomplete adjuvant and injected intramuscularly into lambs. these lambs developed a very high level of immunity to subsequent challenge infection with t. ovis eggs. | 1977 | 927887 |
| vaccination of lambs against infection with taenia ovis. | a trial was designed to test the efficacy of vaccination of lambs against field challenge with t. ovis eggs using 3 different levels of pasture contamination. three concentric paddocks (1.62 hectares each) were used and the inner paddock was heavily contaminated with t. ovis eggs by tethering 2 dogs, experimentally infected with t. ovis, at 5 different points in the paddock over a period of 1 month. four groups of lambs were used. sixty lambs, from a property known to be free of t. ovis for 2 ye ... | 1976 | 962736 |
| fecundity and proglottid release of taenia ovis and t. hydatigena. | the rate of release of proglottids was examined in 19 dogs infected with taenia ovis and 16 infected with t. hydatigena. faeces were examined for proglottids daily until the apparent death of the worms. autopsies were then performed and surviving worms recovered. the number of eggs per proglottid was estimated in 12 t. ovis and 12 t. hydatigena from natural infections. in dogs infected with t ovis the time of first recovery of proglottids after infection varied from 44 days to more than 126 days ... | 1976 | 987771 |
| the effect of corticosteroids on the cysticerci of taenia ovis in sheep. | the effect of two synthetic corticosteroids on the cysticerci of taenia ovis in sheep has been investigated. dexamethasone given from four days before infection had no effect on the number of cysticerci recovered but did enhance their survival. when betamethasone was used there was an increase in the number of cysts compared with controls. corticosteroids given for 12 days after infection did not increase the number of viable cysticerci recovered at autopsy three months later but when treatment ... | 1976 | 1025638 |
| the role of the tasmanian devil in the epizootiology of ovine cysticercosis and echinococcosis. | tasmanian devils were fed infective cystic material of taenia ovis, t. hydatigena and echinococcus granulosus and later killed and examined for adult tapeworms. no infections with t. ovis were found after feeding 23 cysts to 8 devils. t. hydatigena were recovered from 5 to 41 devils and 10 worms established after feeding 417 cysticerci. no e. granulosus were recovered from any of 3 devils. specimens of t. hydatigena appeared to develop as well in devils as in dogs. from these and previous result ... | 1976 | 1035096 |
| muscular cysticercosis from taenia ovis in feedlot lambs. | from a colorado enterprise annually fattening about 50,000 lambs, 3,664 animals were examined for muscular cysticercosis. of the total number, 406 (11.1%) were parasitized, wheras an estimated 0.1% of colorado lamb carcasses are infected. of the infected lambs, 60% had cardiac lesions. two types of cysticerci-viable and degenerated-were found. the lambs probably acquired infection from grazing summer ranges and pastures where coyotes or dogs infected with taenia ovis contaminated the forage. | 1975 | 1184435 |
| the location of taenia pisiformis, taenia ovis and taenia hydatigena in the gut of the dog and its effect on net environmental contamination with ova. | autopsy of dogs 56 days after infection with either t. pisiformis, t. ovis or t. hydatigena showed that these worms could be found attached at any point along the length of the small intestine, but were most commonly in the anterior half. the mean relaxed lengths of t. pisiformis, t. ovis and t. hydatigena were 107 cm, 156 cm and 177 cm respectively. attached gravid proglottides contained a mean of 41 000 eggs each in t. pisiformis, 31 000 eggs in t. hydatigena and 95000 eggs in t. ovis, whereas ... | 1975 | 1210528 |
| ultrastructural studies of the cysticercoid of moniezia expansa (anoplocephalidae) with special reference to the development of the cyst. | cysticercoids of the sheep tapeworm moniezia expansa have been grown in oribatid mites in the laboratory. three species of mite became infected: xenillus tegeocranus, platynothrus peltifer and euzetes globulus, the latter not previously recorded as a host of anoplocephalines. cysticercoids aged 15 and 28 weeks were examined with the electron microscope. four distinct types of cells were found in the 15-week cysticercoid. subsequent cyst development involves a transformation from a cellular to a ... | 1976 | 1258526 |
| the primary structure of neuropeptide f (npf) from the garden snail, helix aspersa. | neuropeptide f (npf), originally isolated from the sheep tapeworm, moniezia expansa, consists of 39 amino acid residues terminating in a phenylalaninamide. an analogous neuropeptide has been isolated and sequenced from extracts of circumoesophageal ganglia of the garden snail, helix aspersa. this neuropeptide exhibits partial primary structural similarity to members of the vertebrate neuropeptide y (npy)/pancreatic polypeptide (pp) superfamily. npf is thus of widespread occurrence in the nervous ... | 1992 | 1472263 |
| comparison of an indirect haemagglutination assay and an elisa for diagnosing fasciola hepatica in experimentally and naturally infected sheep. | an enzyme-linked immunosorbent assay (elisa) with somatic (s) or excretory-secretory antigens (es) was compared with an indirect haemagglutination assay (iha) for ability to detect antibodies against fasciola hepatica in sheep. the specificity of both assays was determined by testing sera collected from sheep experimentally or naturally mono-infected with fasciola hepatica, haemonchus contortus, ostertagia circumcincta, cooperia curticei, taenia ovis, eimeria spp., trichostrongylus vitrinus, tri ... | 1992 | 1485406 |
| echinococcus granulosus: use of an intermediate host mouse model to evaluate sources of protective antigens and a role for antibody in the immune response. | a balb/cj mouse model was used to determine which stage of the e. granulosus life cycle possessed the most potent protective antigens. mice were immunized with crude extracts of protoscoleces, brood capsules, cyst fluid, adult worm tissue, eggs or oncospheres and then challenged intraperitoneally with 600 activated oncospheres. sonically disrupted oncospheres induced the highest levels of protection (greater than 90%) at doses greater than or equal to 10(3) oncosphere equivalents per mouse. high ... | 1992 | 1644518 |
| cestode vaccines. | studies over the past 20 years have clearly shown the potential for developing vaccines against larval cestode infections of man and animals. the important larval cestode infections of man (echinococcus granulosus--hydatidosis: taenia solium--cysticercosis) involve domesticated animals as intermediate hosts in their natural life-cycles. these animals develop strong immunity against reinfection, and immunity can be artificially induced by vaccination with oncosphere antigens. a major stumbling bl ... | 1991 | 1822908 |
| the prevalence of the larval stage of taenia ovis at the port elizabeth abattoir. | the prevalence of the larval stage of taenia ovis and the origin of the animals were recorded for a period of 39 days at the port elizabeth abattoir. cysticercosis was more frequently found in the m. triceps brachii of sheep, but the actual cysticerci were fewer than those found in the semimembranosus, semitendinosus, adductor, biceps femoris, vastus, rectus femoris and gracilis group of muscles of the hind limb (leg muscles). most animals originated from farms in the oudtshoorn district. | 1991 | 2051440 |
| cloning and expression of taenia ovis antigens in escherichia coli. | double stranded dna complementary to poly(a)+ mrna from the tapeworm taenia ovis was cut with sau 3a to an average length of about 300 bp and inserted into the bam hi site of the expression plasmids pex1, pex2 and pex3. these plasmids express a hybrid protein derived from a fusion of the cro gene with the lac z gene (truncated at its 5' end by 53 bp) of phage lambda. cloning sites lie downstream from the gene fusion. escherichia coli infected with another plasmid (pci857) bearing the temperature ... | 1988 | 2453801 |
| characterization of cloned antigens of taenia ovis. | fusion proteins derived from recombinant clones of escherichia coli carrying the pex series of expression vectors with cdna inserts of the tapeworm, taenia ovis, were characterized by their antigenicity when administered to mice and sheep. antisera derived from these hosts were shown to react with a number of t. ovis adult worm and oncospheral antigens in western blots, and to bind to oncospheres and worm tissue in fluorescent antibody tests. epitopes produced by two clones (designated t07 and t ... | 1989 | 2478496 |
| recent advances in vaccination against cysticercosis. | progress in recent research towards vaccination against cysticercosis is reviewed briefly. an antigen of taenia ovis has recently been cloned using recombinant dna techniques and this single defined recombinant molecule has been shown to induce high-level protection against challenge infection in sheep. prospects for the application of this discovery in t. ovis for development of effective vaccines against infection with the metacestodes of other taeniid parasites are discussed. the extent and l ... | 1989 | 2488992 |
| taenia saginata and taenia solium: reciprocal models. | taenia solium and taenia saginata are of such close taxonomic relationship that each can be regarded as a model for the other. in these particular species the only available hosts are either humans or large domesticated animals. this has imposed severe safety and/or economic restrictions on the extent of the experimental work which could be attempted. furthermore, there is a limit to the relevance of work with less closely related species such as taenia ovis, taenia taeniaeformis and taenia pisi ... | 1989 | 2488993 |
| vaccination against ovine cysticercosis using a defined recombinant antigen. | cysticercosis caused by larval tapeworms is a major public health problem and a cause of substantial economic losses in the farm-animal industries. taenia ovis in sheep is a particularly important example. immunity to reinfection with the larvae has a central role in regulating natural transmission of the parasites, and vaccination with antigens from the early larval oncosphere stage can induce complete protection against infection. as it is impractical to obtain enough oncospheres for a commerc ... | 1989 | 2648160 |
| population dynamics in echinococcosis and cysticercosis: economic assessment of control strategies for echinococcus granulosus, taenia ovis and t. hydatigena. | an official control programme against echinococcus granulosus and taenia hydatigena has been in operation in new zealand for more than 28 years and against taenia ovis for more than 18 years. this unique effort to control three metazoan parasites at the same time has led to a change from endemic to extinction status for e. granulosus but only a change from hyperendemic to endemic status for t. hydatigena and t. ovis. this has presented problems in determining the most cost-effective future contr ... | 1988 | 3140196 |
| ultrastructure changes at the surface during the early development phases of taenia ovis cysticerci in vitro. | | 1987 | 3583538 |
| [seasonal dynamics of intermediate hosts and survivability of eggs of the sheep tapeworm moniezia expansa (rudolphi, 1810)]. | | 1986 | 3751392 |
| prevalence and public health implications of echinococcus granulosus in rural dogs in eastern nigeria. | prevalence of echinococcus granulosus infection in rural dogs in eastern nigeria was determined. of the 182 dogs examined, 8 (4.4%) had echinococcus granulosus infection. the number of echinococcus granulosus recovered was 80, of which 16 (20%) were from faeces and 64 (80%) were derived from intestinal mucosa. of the localities surveyed, eke-uke had the highest number of infected dogs, 13 out of 38 (34.3%). the females accounted for 94 out of 182 dogs (51.6%) examined, while 98 (53.8%) were male ... | 1986 | 3759352 |
| the prevalence and epidemiology of cestodes in dogs in clwyd, wales. i. farm dogs. | the results of a survey of 51 farms in clwyd carried out between 1981 and 1983 to elucidate the prevalence and epidemiology of dog cestodes are reported. taeniid species were obtained from 24 (17.1%) of the 140 dogs which purged. taenia ovis and t. hydatigena appeared to depend largely on dogs scavenging from carcasses for the perpetuation of their life-cycles, whereas t. multiceps was found only in dogs on two farms where sheep heads formed the main diet of the dogs. dog husbandry in relation t ... | 1985 | 3834846 |
| studies on the prevalence of gastro-intestinal helminths of dogs in calabar, nigeria. | the results of investigational study to determine the prevalence of gastro-intestinal helminths of dogs in calabar, nigeria with particular emphasis on the effects of sex and age of the animals on the occurrence of these endoparasites are presented. of a total of 254 dogs sampled using saturated brime solution floatation method and formalin-ether sedimentation method, 223 (86.97%) were infected. a breakdown of the number of parasitized dogs shows that 180 (70.86%) were positive for ancylostoma c ... | 1985 | 3869127 |
| comparative studies on the infestation of three different breeds of dogs by gastro-intestinal helminths. | the findings of an investigation mounted to evaluate and compare the prevalence of parasitic gastro-intestinal helminth ova in three different breeds of dogs in calabar, nigeria are presented. results of the investigation indicate that in alsatians, 25% infestation with strongyloides stercoralis, 25% toxocara canis, 65% ancylostoma caninum were found, in crossbreed between alsatian and mongrel, 15.56% infestation with strongyloides stercoralis, 10.93% toxascaris leonina, 25% toxocara canis, 68.7 ... | 1985 | 3869810 |
| specific antibody responses in dogs experimentally infected with echinococcus granulosus. | six dogs reared helminth-free were divided into 2 groups. four dogs were infected per os with 200,000 protoscoleces each of echinococcus granulosus and 2 were kept as uninfected controls. all the dogs were kept together until 32 days after infection, when 1 infected dog was killed, its intestine removed and the contents examined to confirm that the infection with e. granulosus had been successful. the remaining 3 infected dogs were transferred to high security housing and their feces inspected d ... | 1986 | 3953947 |
| the survival in sheep and infectivity to dogs of taenia hydatigena and t. ovis in sheep. | lambs were reared from birth to 3 months of age on pasture contaminated with the eggs of either taenia hydatigena or taenia ovis. they were necropsied at 3, 6, 9 or 12 months. almost all larvae that were viable at 3 months continued to survive throughout the experiment. larvae of t. hydatigena were infective to dogs at 3 months, whereas those of t. ovis required more than 3 months to reach the infective stage. | 1985 | 3992875 |
| the use of excretory and secretory antigens of the scolex of taenia ovis for the serodiagnosis of infection in dogs. | the excretory and secretory antigens from the evaginated scoleces of taenia ovis were collected for 3 days in vitro, and used in an elisa test to detect antibodies to t. ovis in the serum of dogs. when tested on sequentially collected sera, diagnostic elisa values could be detected in many dogs 4 wk after infection, and remained for an average of a further 4 wk after worms were removed from dogs with an anthelmintic. using an elisa discriminant value that eliminated all false positives from 70 u ... | 1985 | 3998957 |
| observations on experimental infections of dogs with taenia ovis. | | 1974 | 4424065 |
| letter: the role of the fox as a host for taenia ovis and taenia hydatigena in australia. | | 1974 | 4477469 |
| taenia ovis in the tasmanian devil. | | 1972 | 4655392 |
| hydatidosis and cysticercosis. 6. acquired resistance by the ewe and its effect on prenatal infections with taenia ovis in the lamb. | | 1973 | 4748337 |
| passive protection of lambs against infection with taenia ovis via colostrum. | | 1974 | 4819468 |
| immunity produced against taenia ovis and t. taeniaeformis infection in lambs and rats following in vivo growth of their larvae in filtration membrane diffusion chambers. | | 1971 | 5090965 |
| a field trial with bunamidine hydrochloride as an anthelmintic in the eradication of echinococcus granulosus, taenia hydatigena and taenia ovis in new zealand. | | 1970 | 5273618 |
| the effect of freezing on the viability of taenia ovis cysts. | | 1971 | 5286931 |
| taenia ovis in a cat. | | 1970 | 5529759 |
| successful vaccination of lambs against infection with taenia ovis using antigens produced during in vitro cultivation of the larval stages. | | 1971 | 5558548 |
| chemical composition of embryophoric blocks of taenia hydatigena, taenia ovis, and taenia pisiformis eggs. | | 1966 | 5949306 |
| chemical ovacidal measures as applied to taenia hydatigena, taenia ovis, taenia pisiformis, and echinococcus granulosus. | | 1967 | 6067383 |
| antibody responses of experimentally infected lambs to antigens collected during in vitro maintenance of the adult, metacestode or oncosphere stages of taenia hydatigena and taenia ovis with further observations on anti-oncospheral antibodies. | the antigenicity and specificity of crude antigens collected during the in vitro maintenance of taenia hydatigena and t. ovis, excretory/secretory (es) antigens, were assessed in a peroxidase microenzyme-linked immunosorbent assay (elisa), using sera from lambs given experimental monospecific infections with t. hydatigena, t. ovis, echinococcus granulosus or fasciola hepatica. es antigens of larval cysts of t. ovis and t. hydatigena were less reactive than those of adult or oncosphere stages. st ... | 1982 | 6180565 |
| the use of a water-in-oil adjuvanted vaccine in an attempt to immunize lambs against taenia ovis cysts in the presence of maternal antibody. | | 1984 | 6469451 |
| [prevalence of oribatid mites on sheep pastures and their role as intermediate host for the sheep tapeworm moniezia expansa]. | | 1984 | 6487259 |
| haematological and serological data from dogs raised worm-free and monospecifically infected with helminths. | pups free from helminth infection were obtained by treating the bitch prior to parturition, and the bitch and her pups after whelping, with oxfendazole and praziquantel. they were then housed in an area free from contamination with helminth eggs. freedom from infection was confirmed by necropsy examinations and by serological tests using the enzyme linked immunosorbent assay (elisa). groups of pups were infected with toxocara canis, echinococcus granulosus, taenia ovis, t. pisiformis and t. hyda ... | 1984 | 6525114 |
| intestinal cestodes of stray dogs in jordan. | five species of cestodes namely echinococcus granulosus, taenia hydatigena, taenia pisiformis, taenia ovis and dipylidium caninum were recovered post mortem from 120 out of 173 stray dogs collected from the 5 governorates of jordan during the period june 1979 to november 1980. twenty-five of the examined dogs (14%) were found to be infected with e. granulosus, 79 (46%) with t. hydatigena, 14 (8%) with t. pisiformis and 5 (3%) with t. ovis. dipylidium caninum was encountered in 33 (19%) of the ex ... | 1984 | 6539037 |
| [histokinesis in experimental ovine cysticercosis]. | the dynamic of the morphologic changes taking place in the viscera of lambs was followed up in lambs with an experimental cysticercus ovis infection. the tests were carried out with 6 two-month-old lambs infected with 30,000 ova of taenia ovis each. the animals were killed on the 2nd, 4th, 12th, 54th, and 70th day of infection, taking notes on the gross and microscopic changes in the internal organs. it was found that in the predilection sites of localization the migrating oncospheres were trans ... | 1984 | 6740931 |
| cysticerci of taenia ovis krabbei moniez, 1879, in the brain of moose, alces alces (l.) in maine. | | 1983 | 6887439 |
| prepatent period of taenia ovis in dogs. | | 1980 | 6935556 |
| vaccination of sheep against taenia ovis. the response to various dose rates of antigens obtained by incubation of oncospheres in vitro. | activated oncospheres of taenia ovis were incubated in vitro for 14 days and the antigens produced were collected and concentrated. tenfold serial dilutions of the antigens were emulsified with freund's incomplete adjuvant and injected intramuscularly into sheep. when subsequently challenged with t ovis eggs, these sheep displayed a level of immunity that was related to the amount of antigen injected. the calculated dose-response curve indicated that for the vaccine to be 90 per cent effective, ... | 1982 | 7100652 |
| the use of a cathodic antigen in the immunoelectrophoretic serodiagnosis of echinococcus granulosus in sheep. | an antigen was prepared from purified sheep hydatid-cyst fluid by gel-filtration on sephadex g-200 followed by chromatography on deae-cellulose. in each case the first-peak material was used. this antigen, which migrated cathodically, was concentrated and used in immunoelectrophoretic analyses of 4x concentrated sera from sheep experimentally and naturally infected with echinococcus granulosus and from uninfected sheep. of 34 sheep with e. granulosus infection 31 were positive with the cathodic ... | 1982 | 7123842 |
| [localization of c. ovis in experimentally infected lambs]. | studied were the distribution of cysticercus ovis in cadavers of lambs experimentally infected with various amounts of taenia ovis ova, the ratio between superficially localized cysticerci and those found deep in the individual groups of muscles and organs, and the ratio between alive and dead cysticerci. the tests were carried out with thirteen lambs treated as follows: 2 animals were infected with 1000 t. ovis ova each; 3 were infected with 4000 ova each; 4--with 7000 ova each; and 4--with 30 ... | 1982 | 7168145 |
| [effect of an experimental infestation with cysticercus ovis on serum enzyme activity in lambs]. | the changes were followed up that take place in the activity of the more important serum enzymes, such as glutamate oxalacetate transaminase, glutamate pyruvate transaminase, serum dehydrogenase, malate dehydrogenase, lactate dehydrogenase, creatine phosphokinase, and alkaline phosphatase, as well as aldolase in the development of the experimental infection with various doses of taenia ovis eggs in lambs. used were 14 two-month-old lambs divided into test groups of 4 animals each and a control g ... | 1982 | 7170768 |
| [hematological and biochemical changes in lambs experimentally infested with cysticercus ovis]. | the effect was followed up of the experimental infection of cysticercus ovis on some hematological and biochemical indices in lambs. the experiments were carried out with a total of 14 two-month-old lambs divided into 3 test groups of 4 animals each and a control group of 2 animals. the lambs of the first group were infected with 4000 taenia ovis eggs, those of the second group--with 7000 t. ovis eggs, and those of the third group--with 30 000 eggs. both the hematological and the biochemical inv ... | 1982 | 7170769 |
| immunisation of lambs against infection with taenia ovis using an extract of taenia ovis eggs. | | 1981 | 7198282 |
| the chemical removal of embryophoric blocks from eggs of taenia ovis and taenia hydatigena prior to in vitro cultivation. | | 1982 | 7200517 |
| anti-oncospheral antibodies in the serum of lambs experimentally infected with either taenia ovis or taenia hydatigena. | using a peroxidase micro-enzyme--linked--immunosorbent assay (elisa) method anti-oncospheral antibodies were demonstrated in sera from four lambs after primary and challenge infections with taenia ovis or t. hydatigena. antibodies assayed using homologous oncospheral antigen (oa) reached a peak by 3-4 weeks after primary infection and at 1-3 weeks post-challenge infection, but waned to pre-infective, "background" levels by 8-12 weeks after each infection. antibodies assayed against antigens in s ... | 1981 | 7210817 |
| development of early larval stages of taenia ovis in vitro using a cell monolayer. | an in vitro culture system incorporating a cell monolayer was used to induce most activated oncospheres of taenia ovis to enter post-oncospheral development. seven distinct developmental phases were recognized during the 23 days required for development to reach a stage similar to that achieved after 14 days in vivo. secretions or tegumental substance were released during each developmental phase. | 1980 | 7422364 |
| hybridoma antibody immunoassays for the detection of parasitic infection: attempts to produce an immunodiagnostic reagent for a larval taeniid cestode infection. | attempts have been made to produce discriminatory immunodiagnostic reagents for infections of sheep with the larval stages of taenia hydatigena, taenia ovis and echinococcus granulosus. using cells from mice hyperimmunized with t. hydatigena antigenic preparations for fusion, a hybridoma antibody which bound to t. hydatigena larval antigens, but not to t. ovis or e. granulosus larval antigens, was selected. a competitive radioimmunoassay using the labelled hybridoma antibody and a crude parasite ... | 1980 | 7436880 |
| transmission dynamics of echinococcus granulosus, taenia hydatigena and taenia ovis in sheep in uruguay. | a base-line survey was carried out on the transmission dynamics of echinococcus granulosus, taenia hydatigena and taenia ovis in sheep in the department of florida, uruguay. mean life expectancy at birth of the sheep population in this department was estimated at 3.5 and 4.8 years for male and female sheep, respectively. both intensity and prevalence of e. granulosus infection increased with age providing no evidence that immunity was acquired in the population. this revealed that e. granulosus ... | 1995 | 7558566 |
| the influence of gamma inulin and algammulin on the immune response in sheep to a recombinant antigen of taenia ovis. | gamma inulin and algammulin, two new adjuvants, were examined and compared with alum and freund's complete/incomplete adjuvant (fca/fia), for potentiation of cell-mediated immunity (cmi) and humoral immunity in sheep to a recombinant taenia ovis antigen. the ability to protect sheep when challenged with live t. ovis eggs was also assessed. the results showed that gamma inulin and algammulin induced a cmi response which was comparable to the fca/fia and alum groups and significantly higher than t ... | 1995 | 7639010 |
| comparison of two enzyme immunoassays for the detection of haemonchus contortus infections in sheep. | two enzyme-linked immunosorbent assays (elisa) using either excretory/secretory (es) products or crude somatic antigens (csa) of adult haemonchus contortus were compared for their ability to detect antibodies against h. contortus in sheep. serum samples obtained from a group of 32 h. contortus mono-infected sheep were tested in the two elisas and the obtained data were compared with the results of the faecal examinations of these sheep. the first sheep became patent 3 weeks post infection (p.i.) ... | 1995 | 7660570 |
| a defined antigen for the serodiagnosis of taenia ovis infections in dogs. | the evaginated scolex of taenia ovis secretes an antigen complex into defined culture medium that has been used to develop a cestode-specific enzyme-linked immunosorbent assay (elisa). we now describe an immunoblot test for antibodies to t. ovis based on the recognition of a 94-kda antigen band in the sodium dodecyl sulfate-polyacrylamide gel electrophoresis profile of t. ovis scolex secretions. the test was specific for cestode infections in dogs and was 82% sensitive for the recognition of t. ... | 1995 | 7776128 |
| taenia ovis recombinant vaccine--'quo vadit'. | several years have elapsed since the publication by johnson et al. (1989) of the cloning of a recombinant antigen from the cestode parasite taenia ovis which stimulated high levels of protective immunity in sheep. a great deal of subsequent research and development was necessary to bring the fledgling vaccine to the point of being a registered commercial product. the results of these subsequent studies are dealt with briefly in this paper, including the results of field trials. the t. ovis vacci ... | 1995 | 7784129 |
| modelling of parasitic populations: cestodes. | the philosophy of mathematical modelling as it applies to the epidemiology of cestode populations is reviewed. a model provides, via the "threshold theorem", a criterion for deciding in advance if a control programme can succeed in eradicating the parasite. in order to use this criterion it is necessary to have an estimate of the basic reproduction ratio, r0, which can only be obtained if reliable epidemiological data are available before the control programme is started. a model has been used t ... | 1994 | 7846848 |
| metacestodes of sheep with special reference to their epidemiological status, pathogenesis and economic implications in fars province, iran. | this study was undertaken over a period of 2 years. of 7992 sheep examined, 2088 (26.12%), 2266 (28.36%), 15 (0.19%) and 738 (9.8%) were infected with echinococcus granulosus, taenia hydatigena, taenia ovis and taenia multiceps metacestodes, respectively. the prevalence was higher in animals belonging to migratory tribal people than in those owned by the permanent residents of fars province. dogs in the former group also showed significantly higher levels of infection with e. granulosus (33.3%) ... | 1994 | 8171825 |
| vaccination against cestode parasites. | cestodes are tapeworm parasites. infection in the intermediate host with larval (metacestode) parasites causes medically and economically important diseases known as hydatidosis and cysticercosis. immunization against experimental infection with metacestode parasites has been highly successful, in marked contrast with the relative ineffectiveness of vaccines against infection with most parasitic organisms. high levels of immunity against a challenge infection with taeniid cestode eggs can be sti ... | 1993 | 8270273 |
| gnffrfamide: a novel fmrfamide-immunoreactive peptide isolated from the sheep tapeworm, moniezia expansa. | the widespread distribution of fmrfamide-immunoreactivity in platyhelminth nervous systems has been demonstrated immunocytochemically. here we report the isolation and primary structure of the first platyhelminth fmrfamide-related peptide (farp) from the tapeworm, moniezia expansa. the peptide was found to have a molecular mass of 785 da and a primary structure of gly-asn-phe-phe-arg- phe-nh2 (gnffrfamide). acid alcohol extracts of this platyhelminth contained only this single farp which had a p ... | 1993 | 8323531 |
| identification of host-protective antigens of taenia ovis oncospheres. | sheep were fully protected against challenge infection following immunization with a homogenate of t. ovis oncospheres. ultracentrifugation of sonicated oncospheres either alone or in the presence of a range of detergents did not reduce the immunogenicity of the extracts. solubilization of oncosphere extracts in non-ionic detergents or sodium dodecyl sulphate (sds) enabled analysis of host-protective antigens by isoelectric focusing (ief) and electrophoresis in polyacrylamide gels (sds-page), re ... | 1993 | 8468135 |
| characterization of the gene family encoding a host-protective antigen of the tapeworm taenia ovis. | genomic structure has been determined for a gene encoding a host-protective antigen of the parasitic platyhelminth taenia ovis. an incomplete cdna, known as 45w, encodes the protective antigen. southern hybridisation experiments using 45w cdna as a probe, revealed that the 45w gene was a member of a multigene family. differential southern hybridisation and rapid amplification of cdna end (race) experiments were used to characterise the related genes, allowing the full-length coding region of the ... | 1995 | 8577320 |
| developmental regulation of taenia ovis 45w gene expression. | | 1995 | 8577337 |
| gastrointestinal helminths of arctic foxes (alopex lagopus) from different bioclimatological regions in greenland. | nine species of gastrointestinal helminths were recovered from 254 arctic foxes (alopex lagopus) from 8 different localities in greenland. prevalences of infection with the helminth species differed from area to area: toxascaris leonina (39-68%), strongyloides stercoralis (0-14%), mesocestoides lineatus (0-58%), diphyllobothrium dendriticum (0-15%), taenia ovis krabbei (0-70%), cryptocotyle sp. (0-3%), plagiorchis elegans (0-6%), and polymorphus sp. (0-3%). additionally, a taenia species, which ... | 1996 | 8627489 |
| identification and cdna cloning of two novel low molecular weight host-protective antigens from taenia ovis oncospheres. | oncosphere antigens of taenia ovis were solubilised in sodium dodecyl sulphate and separated by electrophoresis in polyacrylamide gels (sds-page). antigen-containing gel fractions cut from the region covering 18-12 kda were shown to be highly immunogenic in sheep challenge experiments. specific antisera against 2 candidate antigens at 18 and 16 kda were used to screen a cdna library prepared from t. ovis oncosphere mrna. recombinant proteins selected with antibody to the 16 and 18 kda native ant ... | 1996 | 8690544 |
| parasite vaccine development: large-scale recovery of immunogenic taenia ovis fusion protein gst-45w(b/x) from escherichia coli inclusion bodies. | genetically modified escherichia coli expressing the taenia ovis fusion protein gst-45w(b/x) as inclusion bodies were grown in volumes ranging up to 1000 l. bacteria were inactivated by heat or chemical treatment without affecting immunogenicity. the fusion protein was recovered in a highly immunogenic form from washed inclusion bodies and from urea-solubilised inclusion bodies. the fusion protein was found to be stable in solution after storage at 4 degrees c for up to 2 years. vaccines formula ... | 1996 | 8740543 |
| antigen-specific iga-secreting cells induced by peripheral vaccination. | antigen-specific iga-secreting cells were shown to be generated in peripheral lymph nodes following s.c. vaccination. the efferent duct of prefemoral lymph nodes of sheep were cannulated prior to vaccination at a site draining to the cannulated node. vaccines were contained a recombinant protein (taenia ovis 45w-gst) and either incomplete freund's, quil a or al(oh)3 as adjuvant. lymph fluid was examined for the presence of 45w-gst-specific antibody by elisa and antibody-secreting cells by enzyme ... | 1996 | 8799732 |
| vaccination against worm parasites of animals. | the 1990s have seen the culmination of decades of painstaking research with the registration and launch of tickgard (hoechst), a recombinant vaccine against boophilus microplus, and the provisional registration of a taenia ovis vaccine. research continues to hold promise for immunological control of echinococcus, fasciola, haemonchus, trichostrongylus and ostertagia. blood-sucking parasites (e.g. ticks and h. contortus) are susceptible to control by vaccines containing 'novel' or 'concealed' ant ... | 1996 | 8893462 |
| [vaccines against parasitic diseases of domestic animals]. | this review presents short information on the present status and some future perspectives of vaccination against parasitoses of domestic animals. for the control of such parasitoses in some european countries only a few vaccines are registered: paracox and livacox (for coccidiosis in chickens), toxovax (for toxoplasmosis in sheep), pirodog (for babesiosis in dogs) and dictol (for dictyocaulosis in cattle). these are live vaccines containing attenuated parasites, except pirodog. as a world-wide i ... | 1996 | 9012013 |
| nucleic acid vaccination of sheep: use in combination with a conventional adjuvanted vaccine against taenia ovis. | this report describes the use of a nucleic acid vaccine in a large outbred animal species both alone and in combination with a conventionally adjuvanted vaccine. the gene encoding a host-protective antigen (45w) from the sheep parasite taenia ovis was cloned into the expression vector pcdna3 and the resultant plasmid termed pcdna3-45w. eleven of 15 sheep injected either intramuscularly or intradermally with pcdna3-45w mounted a serum antibody response to 45w which for both routes of injection wa ... | 1997 | 9046433 |
| taenia ovis: copy number of genes encoding host-protective antigens determined by competitive pcr. | | 1997 | 9149244 |
| urea/dtt solubilization of a recombinant taenia ovis antigen, 45w, expressed as a gst fusion protein results in enhanced protective immune response to the 45w moiety. | vaccination and challenge infection experiments were conducted in sheep using different forms of a recombinant protein (45w) from the cestode parasite taenia ovis. 45w was expressed in escherichia coli as a fusion protein with glutathione-s-transferase (45w-gst) and was produced as both soluble protein and insoluble inclusion bodies. vaccination of animals with either the soluble or inclusion body derived protein resulted in the immune response being predominantly directed to the gst moiety of 4 ... | 1997 | 9160512 |
| sequence analysis of a gene family encoding taenia ovis vaccine antigens expressed during embryogenesis of eggs. | the 45w vaccine antigen of the cestode parasite taenia ovis is a member of a small gene family estimated to comprise six members. all six genes were cloned and characterised. nucleotide sequence analysis of the different family members identified a set of closely related genes exhibiting between 75.2 and 98.6% dna sequence homology. intron/exon structure for the various genes constituting the family was found to be conserved. open reading frame analysis and pairwise alignments of predicted polyp ... | 1997 | 9178269 |
| sequential nucleic acid and recombinant adenovirus vaccination induces host-protective immune responses against taenia ovis infection in sheep. | sheep were immunized with a protective recombinant antigen (45w) from the cestode parasite taenia ovis using three different vaccine delivery systems, either alone or in different combinations. the dna encoding 45w was cloned into the expression plasmid pcdna 3 and an ovine adenovirus to create nucleic acid and recombinant viral vector vaccines, respectively. sheep received two vaccinations with various combinations of these two delivery systems and/or purified recombinant 45w protein in a conve ... | 1997 | 9194099 |
| rates of reinfection with echinococcus granulosus, taenia hydatigena, taenia ovis and other cestodes in a rural dog population in uruguay. | a survey was undertaken to determine both the prevalence of, and reinfection rates with echinococcus granulosus and other cestodes in the department of florida, uruguay. baseline prevalence was determined in 303 rural dogs which then, in 4 groups, were re-examined 2, 4, 8 or 12 months later. baseline prevalences for e. granulosus, taenia hydatigena, taenia ovis and dipylidium caninum were 13.2, 13.9, 2.3 and 13.2%, respectively. the frequency distribution of e. granulosus was over-dispersed. dog ... | 1996 | 9198601 |
| immune responses associated with protection in sheep vaccinated with a recombinant antigen from taenia ovis. | this paper describes the evaluation of the protective antibody response of sheep to vaccination against taenia ovis infection with a defined recombinant antigen (45w). sera from 181 vaccinated sheep, collected prior to experimental challenge with t.ovis, were assessed for 45w specific iga, igg, igg1, igg2 and igm levels and these results correlated with protection data. there were significant relationships (p < 0.001) between igg, igg1 and igg2 titres and protection. serum iga levels did not cor ... | 1996 | 9223175 |
| host-protective fragments and antibody binding epitopes of the taenia ovis 45w recombinant antigen. | the protective efficacy of a recombinant taenia ovis vaccine antigen, 45w, was compared in sheep vaccine trials with antigen expressed by the full length 45w cdna and by incomplete copies of the cdna. vaccine, trials were also carried out using antigen expressed by a cdna (45s) having a sequence similar, but not identical, to 45w. stability of the 45w antigen expressed in escherichia coli was found to be increased after deletion of cdna sequence encoding 19 cooh-terminal amino acids. this trunca ... | 1996 | 9226688 |
| the use of recombinant ovine il-1beta and tnf-alpha as natural adjuvants and their physiological effects in vivo. | in the present study we have investigated the use of recombinant ovine il-1beta and tnf-alpha both alone and in combination, as natural adjuvants in vaccination trials in sheep. initial experiments were conducted to investigate the physiological effects of the cytokines in vivo and determine what dose could be administered without adverse pyrogenic effects. even at the maximum dose tested (100 microg) the only significant physiological effect was a transient increase in body temperature of appro ... | 1998 | 9619487 |
| a taenia solium oncosphere protein homologous to host-protective taenia ovis and taenia saginata 18 kda antigens. | a taenia solium cdna (tsol-18) encoding a protein with close homology to host protective oncosphere antigens from taenia ovis (to18) and taenia saginata (tsa-18) is described here. tsol-18 was cloned from mrna obtained from hatched and activated oncospheres of t. solium. the high level of predicted amino acid sequence homology among tsol-18 and other host protective taeniid antigens suggests that the protein expressed by tsol-18 may be capable of being used as a vaccine against t. solium infecti ... | 1998 | 9650055 |
| antibody and cytokine responses in efferent lymph following vaccination with different adjuvants. | the cannulated efferent lymph node in sheep was used to examine the effect of different adjuvants on the antibody and cytokine responses following sub-cutaneous vaccination with a recombinant taenia ovis antigen (45 w). vaccination with quil a elicited relatively higher levels of igm than did ifa or al(oh)3. in general, 45 w specific igg1 and igg2 titres were higher and maintained for longer periods of time in lymph from sheep vaccinated with ifa and lower and shorter lived in animals which rece ... | 1998 | 9656452 |
| modelling the prevalence of echinococcus and taenia species in small ruminants of different ages in northern jordan. | a base-line survey of echinococcus granulosus, taenia hydatigena and t ovis were undertaken in order to investigate the transmission dynamics of these parasites in northern jordan. intensity of e. granulosus infection, in sheep, increased with age in a linear fashion whilst the asymptotic prevalence was one. this implied that e. granulosus is in an endemic steady state with no evidence of protective immunity in the intermediate host. the mean number of cysts increased by 1.66 per year with appro ... | 1998 | 9777724 |
| synthetic peptide antigens induce antibodies to taenia ovis oncospheres. | sheep immunised with the taenia ovis recombinant 45w antigen are protected from infection with the parasite. two peptides were synthesised corresponding to putative host-protective regions at the n- and c-termini of 45w. sera from sheep immunised with 45w or related recombinant proteins reacted strongly with the n-terminal peptide. approximately 40% of the antibody directed against 45wb/x, a truncated form of 45w, was found to be directed against the n-terminal peptide sequence. sheep were immun ... | 1999 | 10195787 |
| vaccination against taenia solium cysticercosis in pigs using native and recombinant oncosphere antigens. | pigs were immunised with antigens derived from taenia solium oncospheres or with a pool of three recombinant antigens from taenia ovis, and subsequently challenged with t. solium eggs. the native oncosphere antigens induced 83% protection against viable, and 89% protection against the total number of cysticerci established following the challenge infection. immunisation with the recombinant t. ovis antigens induced 93% protection against the establishment of viable cysticerci, and 74% protection ... | 1999 | 10428641 |