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proline in fascioliasis: iii. activities of pyrroline-5-carboxylic acid reductase and pyrroline-5-carboxylic acid dehydrogenase in fasciola. 19764402
the role of carbohydrates in the biology of the liver fluke, fasciola gigantica: effects of temperature and ph upon glucose uptake. 19755167
some aspects of the action of hexachlorophene in rats infected with fasciola hepatica. 19768253
general survey of the present distribution of helminths in iran and comparison with those of past years.in this paper the literature on helminthiasis in iran is reviewed and the distribution, prevalence and intensity of infections caused by helminths are discussed. a comparison of prevalence and intensity of infections was accomplished in the recent 10 years by the author. hookworm infections are found mostly in the north along the caspian sea, but its prevalence is declining. trichostrongylus are found in iran with a high prevalence in central country. schistosoma haematobium is found in one prov ...19768977
fasciola hepatica: the presence of particle-associated and soluble nonspecific acid phosphatases. 19769306
fasciola hepatica and fasciola gigantica: total cholinesterase, characteristics, and effects of specific inhibitors. 197716762
the regional distribution of dopamine and norepinephrine in schistosoma mansoni and fasciola hepatica. 197723928
the efficiency of diamphenethide in controlling natural infestations of fasciola hepatica in lambs.the activity of diamphenethide against natural infestations of fasciola hepatica in fattening lambs was assessed on a farm with a noted history of fascioliasis. the main experimental groups, two treated and one control, grazed known contaminated pastures for a twelve week period after which they were removed to fluke-free areas to await slaughter. three groups of four tracer lambs were each grazed for three successive four week periods with the main flock to determine continuity of infection. a ...197824284
effect of diamfenetide on experimental infections of fasciola hepatica in lambs: anthelmintic and clinical investigations.the activity of diamfenetide (n,n'-oxybis(2,1-ethan diyloxy-4,1-phenylene) bis acetamide) was studied in lambs experimentally inoculated with fasciola hepatica. the drug was given orally at a dose level of 100 mg/kg either 1,3,5,7, or 9 weeks postinoculation. it was 100% effective 1, 3, and 5 weeks postinoculation, 73% effective 7 weeks postinoculation, and 57% effective 9 weeks postinoculation. serum gamma-glutamyl transpeptidase activity remained normal in all lambs for 5 weeks after infection ...197825319
gldh and gamma glutamyl transferase activities in the serum of healthy and liver-fluke infested cattle. 197825485
resistance to fasciola hepatica in cattle. ii. biochemical and morphological observations. 197826701
the influence of some physico-chemical factors on the host-finding capacity of fasciola hepatica miracidia. 197826708
the metabolic profile of adult fasciola hepatica obtained from rafoxanide-treated sheep.sheep infected with adult fasciola hepatica were drenched with rafoxanide. at 4, 8, 16 and 24 h after drenching the sheep were killed and the flukes removed, washed and rapidly frozen in liquid nitrogen. the content of key metabolites in the fermentation pathway were determined and compared with those in control f. hepatica, whose hosts were not treated with rafoxanide. rafoxanide decreased glycogen, malate, nadh and atp levels. the level of other metabolites in the pathway increased for the fir ...197826902
effects of intravenous salmonella dublin on cattle at different stages of fasciola hepatica infection. 197827541
recrudescence of fascioliasis (hepatic distomatosis): the result of a rainy summer?. 197827758
experimental cross infections of fasciola hepatica in lambs and calves.the effects of experimental infections with fasciola hepatica of ovine and bovine origin in homologous and heterologous hosts and in uninfected controls were compared; groups comprised 5 animals each. the effects of the infections were monitored by biweekly determinations of packed cell volumes (pcv), serum glutamic oxaloacetic transaminase (sgot), serum glutamic pyruvic transaminase (sgpt), gamma glutamyl transpeptidase (ggt), serum iron, bilirubin levels, alkaline phosphatase (alp) and total s ...197828389
adenylate cyclase from fasciola hepatica. 2. role of guanine nucleotides in coupling adenylate cyclase and serotonin receptors. 197830890
concurrent daily infection of calves with fasciola hepatica and ostertagia ostertagi.three groups of calves were infected daily with either 1500 ostertagia ostertagi larvae, 20 fasciola hepatica metacercariae, or 1500 o ostertagi plus 20 f hepatica metacercariae. weekly measurements were taken of calf weight, faecal egg output, plasma concentrations of albumin, plasma activities of sorbitol dehydrogenase, gamma glutamyl transpeptidase and pepsinogen and standard haematological indices. calves were killed either 10 or 21 weeks after daily infections began. f hepatica infection ha ...197834850
divergent results in radial immunodiffusion. iii. isolation and specificity test of class-specific antibodies.a method is described for the isolation of antibodies specific for immunoglobulin class determinants from antisera containing antibodies both for the immunoglobulin class and for its subclasses, using affinity chromatography. the model system used is bovine igg with its subclasses igg1 and igg2, together with antisera raised in goats and sheep. the specificity of the isolated class-specific antibodies was demonstrated by double diffusion (di) and radial immunodiffusion (rid). the levels of total ...197937277
[choledochal obstruction due to fasciola hepatica (author's transl)].a 45-year-old woman was admitted in july, 1976 with an acute cholecystitis without jaundice. she had suffered from hepatic colic without fever, jaundice, diarrhea or allergic episodes for the past 8 years. the physical examination only revealed an elective pain on the cystic point. laboratory data were unremarkable, except for a 12 percent eosinophils. the cholecystogram showed a cholelithiasis. the lithiasis was confirmed during the surgical operation and a fasciolasis was diagnosed after one a ...197943437
isolation and some properties of an acid protease from fasciola hepatica.a new protease, detected in an extract of fasciola hepatica, was isolated and partly purified. the ph optimum for the cleavage of denaturated haemoglobin by the enzyme is ph 3.0. this proteolytic activity is inhibited by diazoacetylnorleucine methyl ester, pepstatin, the pepsin inhibitor from ascaris suum, and phenylalanine. the cathepsin d inhibitor from potatoes, edta, mercaptoethanol and the inorganic salts tested have no inhibitory effect. the cleavage of the b-chain of oxidized insulin by e ...197944401
[changes in the composition of bovine serum during invasion by distomata genus paramphistomum and fasciola hepatica]. 197549982
nonspecific reactions with the intradermal test for hydatidosis in persons with other helminth infections.a partially purified echinococcus antigen solution, prepared by boiling hydatid cyst fluid, was used in the intradermal test for hydatid disease in a peruvian population. an unexpectedly high rate of positive reactions and poor agreement with serological tests suggested the presence of some agent(s) which produced cross-reactions with echinococcus granulosus antigens in the intradermal test. testing of hospital patients infected with a variety of helminths demonstrated nonspecific reactions in p ...197553018
can we vaccinate against schistosomes? 197662680
further observations on the specificity of antigen 5 of echinococcus granulosus.the presence of ige antibodies to antigen 5 of echinococcus granulosus was detected by means of radioimmunoelectrophoresis in the sera of two of six patients infected with e. multilocularis. sera from three of these patients gave a precipitin band in gel diffusion tests identical to that produced by a monospecific rabbit anti-e. granulosus antigen 5 serum, when tested against whole hydatid fluid. sera from 19 individuals infected with fasciola hepatica, 20 with schistosoma mansoni, and 5 with wi ...197768114
[studies on the modification of performance and metabolic processes in sheep through experimental fasciola hepatica infections considering low infestation intensity].low experimental infections with fasciola hepatica (8...17 flukes per animal) in sheep, 6...12 months old, caused less gain weight over a period of 22 weeks. changes in the number of erythrocytes and in the hb-concentration were not found. in contrast to the controls the infected sheep showed strong fluctuations in the number of lymphocytes. 3 weeks post infect. a significant eosinophilia was observed, which continued until the end of the investigation. with the beginning of the fourth week post ...197773356
human fascioliasis. 197878394
methods of preparation of fossil bone samples for light and transmission electron microscopy.the methods described make possible the preparation of fossil samples for light nad transmission electron microscopy. to obtain good images with ground sections of bone, both the grinding process and classical bone staining techniques were modified, the latter by staining already made ground sections. ground sections of suitable thickness were etched with a 4:1 mixture of 5% hno3 and 2% oso4 at a lowered temperature, thereby obtaining isolated osteocytes with a complete network of processes. usi ...197880848
effect of some phosphodiesterase inhibitors on adenylate cyclase from the liver fluke, fasciola hepatica. 197988227
comparative evaluation of 7 helminth antigens in the enzyme-linked immunosorbent assay (e.l.i.s.a.).112 sera from europeans with parasitologically proven helminthiasis were tested in the enzyme-linked immunosorbent assay (e.l.i.s.a.) against 6 crude extracts of various helminths (2 of adult worms: dipetalonema viteae, fasciola hepatica; 3 of eggs: ascaris suum, toxocara canis, schistosoma mansoni; and of echinococcus granulosus scolices) and against bovine hydatid fluid. each serum was tested simultaneously at a fixed dilution of 1:160 against all antigens. extensive cross-reactions were obser ...197992420
closantel, a new antiparasitic hydrogen ionophore [proceedings]. 197993944
[distomatosis and cardiac disease. apropos of 2 new cases. trials of classification and pathogenetic hypotheses].the authors report two new cases of cardiac disease associated with distomatosis: one case of biventricular fibroblastic parietal endocarditis affecting mainly the left side in a young female of 26 years, and one case of cardiomyopathy with atrial endocardial fibrosis, affecting especially the left ventricle in a man of 47. bearing in mind the five cases reported in the literature, the authors propose a classification of cardiac disorders supposed to be due to distomatosis using three groups (en ...1978105684
[the effectiveness of the drugs dovenix and bilevon(r)-injection against liver fascioliasis (fasciola hepatica, linné 1758) in cattle].dovenix and bilevon-injection (manufactured by specia, france and bayer, west germany, respectively) were tested for their anthelmintic efficacy against fasciola hepatica in cattle. the drugs proved highly effective against both adult and immature flukes. the faeces of the treated animals were negative for f. hepatica eggs when examined 91 days after the treatment. in experiments with rats dovenix and bilevon-injection were tolerated up to eight times and ten times higher doses than the normal t ...1979115128
immunity in schistosoma mansoni using antigens of fasciola hepatica isolated by concanavalin a affinity chromatography.antigens of fasciola hepatica adult worms were chromatographed using concanavalin a-sepharose 4b. two unbound peaks appeared in the inclusion volume (dt-1 and dt-2), and one peak was eluted with alpha-methylglucoside (e1-1). at least seven peaks were obtained by isoelectric focusing of e1-1. the largest of these peaks, with an average pi of 4.0, contained the antigens reactive with antibodies to schistosoma mansoni. mice immunized with dt-2 or e1-1 and challenged with s. mansoni cercariae develo ...1979118932
antimalarial activity of floxacrine (hoe 991) i. studies on blood schizontocidal action of floxacrine against plasmodium berghei, p. vinckei and p. cynomolgi.floxacrine (hoe 991), 7-chloro-10-hydroxy-3-(4-trifluoromethylphenyl)3,4-dihydroacridine-1,9-(2h, 10h) dion, shows a high level of antimalarial action against blood-induced infection of drug-sensitive and drug-resistant lines of plasmodium berghei in mice, rats and syrian hamsters. the drug is also a potent blood schizontocide against drug-sensitive p. vinckei strains in rodents and p. cynomolgi in rhesus monkeys. the cd50/cd90 values against the drug-sensitive p. berghei strain ascertained in t ...1979120142
immunological responses of fluke-infected and fluke-free cattle to salmonella dublin and other antigens.immune responses to heat-killed brucella abortus strain 19 and to ovalbumin were compared in 15 fluke-infected and 15 fluke-free friesian heifers. b abortus was injected 16 weeks and ovalbumin 19 weeks after the oral administration of 1000 metacercariae of fasciola hepatica. agglutinating antibody responses to b abortus were similar in both groups. immediate type hypersensitivity to ovalbumin was apparently suppressed in fluke-infected animals when assessed by active and passive cutaneous anaphy ...1979120572
[lewis blood group activity in fasciola hepatica].lewis blood group specificities are detected in the liver fluke: fasciola hepatica. such specificities had never been reported in nature, other than in the human body. the investigation of this phenomenon might contribute to a better understanding of the mechanisms involved in parasite-host relationship.1979120793
helminth parasitisms among intermingling insular populations of white-tailed deer, feral cattle, and feral swine.helminth infections among free-ranging, intermingling populations of white-tailed deer (odocoileus virginianus), cattle (bos taurus), and swine (sus scrofa) on an island off the georgia coast were studied. of 39 species of helminths collected, 19 were found in deer, 17 in cattle, and 13 in swine. of 28 species of helminths recovered from ruminants, 8, viz, capillaria bovis, cooperia punctata, dictyocaulus viviparus, gongylonema pulchrum, g verrucosum, haemonchus contortus, moniezia benedeni, and ...1975123523
[inhibiting effects of aromatic sulfides, sulfoxides and sulfones on the biochemical systems of fasciola hepatica]. 1975126358
trials with rafoxanide. 8. efficacy of an injectable solution against trematodes and nematodes in cattle.four experiments are described in which the efficacy of an experimental 5% injectable solution of rafoxanide was evaluated against various adult and immature helminths in cattle. subcutaneous injection at a dosage of 3 mg/kg live mass resulted in the following reductions in mean worm burdens: adult fasciola hepatica, 82,6%; adult fasciola gigantica, 99,8% immature paramphistomum microbothrium, 10,1% adult haemonchus placei, 99,6%, third stage h. placei, 73,7%; adult bunostomum phlebotomum, 99,8% ...1977144191
chemotherapy of parasitic worms: new biochemical strategies.many chemotherapeutic agents that are effective against parasitic helminths affect cellular regulatory sites that control motility, metabolism, chemotaxis, and egg formation. serotonin receptors are present in several species of parasitic flatworms and appear to participate in the regulation of motility and carbohydrate metabolism. in fasciola hepatica these receptors are coupled to an adenylate cyclase through a cellular component that requires guanosine triphosphate. serotonin is the most pote ...1979156397
fasciola hepatica human infection. histopathological study of sixteen cases.sixteen human cases of fasciola hepatica infection are described. the liver was involved in 13 cases, the gall bladder in 9 cases and the stomach in 2 cases. lesions containing parasitic remnants or fluke eggs were rarely seen. surface scarring of the liver, scar tracks and granulomas within organs were the most characteristic changes seen and were the most useful for the histopathological diagnosis of the disease. the associated liver, bile and gastric lesions are briefly discussed.1979158874
[histological study of the development of fasciola hepatics in lymnaea truncatula, l. glabra and l. palustris infested at hatching].the development of fasciola hepatica is histologically analysed in limnaea truncatula, l. glabra and l. palustris infested at hatching with a single miracidium. the development of the redial generations of fasciola is not affected by the species of snail host but depends on growth of the snail. in snails of size within 2 mm (considered at the day 49 of infestation to 20 degrees c), the rediae show a delay of maturity compared with rediae observed in snails of higher sizes. the distribution of re ...1978159105
effects of the injectable contraceptive depot medroxyprogesterone acetate in thai women with liver fluke infestation: final results.the effect of the 3-monthly injectable contraceptive depot medroxyporgesterone acetate (dmpa) on liver function and lipids was assessed in thai women both with and without liver fluke (ophisthorchis viverrini) infestation. dmpa administration was started in the immediate postpartum period and women who accepted immediate postpartum iud insertion of sterilization were recruited as a control group. complete 18-month followup results were obtained for 108 dmpa and 106 control fluke-positive subje ...1979161523
the electron transport systems of fasciola hepatica mitochondria. 1975163804
surface ultrastructure and cytochemistry of parasitic helminths. 1975164363
catabolic path of arginine and nad regeneration in the parasite fasciola hepatica. 1975166790
oxidative enzymes in the development of fasciola hepatica l. iv. the activity of oxidases and dehydrogenases in redia.the object of the study was to investigate the occurrence and localization of oxidative enzymes in the redia -- the third larval stage of fasciola hepatica l. the author detected cytochrome oxidase, peroxidase, nadh and nadph tetrazolium reductases (diaphorases), as well as succinate, isocitrate, malate, lactate, alpha-glycerophosphate, glyceraldehyde phosphate, glucose-6-phosphate, 6-phosphogluconate, beta-hydroxybutyrate, l-glutamate, and alcohol dehydrogenases. the presence and localization o ...1975173635
oxidative enzymes in the development of fasciola hepatica l. v. activity of oxidases and dehydrogenases in the cercaria and metacercaria.the object of the study was the investigation of the occurrence and localization of oxidative enzymes in the 4th and 5th larval stages of the liver fluke, i. e. in the cercaria and metacercaria. the following enzymes were detected: cytochrome oxidase, peroxidase, nadh and nadph tetrazolium reductases/diaphorases) as well as succinate, isocitrate, malate, lactate, alpha-glycerophosphate, glyceraldehyde phosphate, glucose-6-phosphate, 6-phosphogluconate, beta-hydroxybutyrate, l-glutamate and alcoh ...1975173636
specific and non-specific phosphatases in the miracidium of fasciola hepatica l.localization and activities of alkaline phosphatase, atpase, 5-nucleotidase, glucose-6-phosphatase, thiamine pyrophosphatase and nucleoside diphosphatase were studied in the miracidium of fasciola hepatica l. except for nucleoside diphosphatase whose activity in the miracidium was not observed, all the enzymes were most active in the archenteron, protonephridia and nerve ganglion. this localization of the reaction intensity allows the inference that the three organs mentioned are sites of both i ...1975173637
[association of hepatic hydatidosis (3 cysts) and choledochal distomiasis]. 1975174214
cyclic amp phosphodiesterase activity in fasciola hepatica (l.) homogenates.the authors have studied the bahaviour of cyclic 3',5'-amp phosphodiesterase in the 2,000 g supernatant of the fascilola hepatica homogenate, under basal conditions and after addition of various substances. dopamine remarkably inhibits the enzyme activity, imidazole causes a strong activation, while serotonin, theophylline, pge1 and pgf2alpha appear to be ineffective.1975174476
fasciola hepatica: ultrastructure and histochemistry of the glycocalyx of the tegument. 1976176054
adenosine cyclic 3',5'-monophosphate in the liver fluke, fasciola hepatica. i. activation of adenylate cyclase by 5-hydroxytryptamine. 1976176567
adenosine cyclic 3',5'-monophosphate in the liver fluke, fasciola hepatica. ii. activation of protein kinase by 5-hydroxytryptamine. 1976176568
regulation of pyruvate kinase and phosphoenolpyruvate carboxykinase activity in adult fasciola hepatica (trematoda). 1976179961
[the effect of newer anthelmintics on fasciola hepatica in experimentally infected rats].the reports deals with the results of testing seven new antihelminthics for fasciola hepatica in the experimentally invaded wistar rat. the greatest influence on juvenile flukes (2 and 4 weeks of age) was exerted by diamphenetid (coriban) applied in a single dose of 100 mg kg-1. hexachlorophene applied in the dose of 50 mg kg-1 showed the highest effect on sexually mature flukes. all the tested antihelminthics of the halogenated salicylanilide group were ineffective on juvenile stages and only s ...1976191977
phosphodiesterase in the liver fluke, fasciola hepatica. 1977202282
hepatic carcinoma with opisthorchiasis.eighty-seven cases of primary hepatic carcinoma associated with opisthorchiasis were reviewed. the prevalence of liver carcinoma among 154 cases of liver fluke infection was 56.6%. the tumours were classified histologically as cholangiocarcinoma in 67 cases (77%), hepatocellular carcinoma in 9 (10.3%), mixed hepatocholangiocarcinoma in 4, squamous carcinoma in 2, and undifferentiated carcinoma in 5. metastases, found in 71 cases, occurred most frequently in the regional lymph nodes and lungs. th ...1978214874
fasciola hepatica: basal infolds and associated vacuoles of the tegument. 1978215443
development of an in vitro technique for cytological investigations of slices of fasciola hepatica: evaluation by morpholigical criteria. 1975236261
carbon dioxide utilisation, and the regulation of respiratory metabolic pathways in parasitic helminths. 1975237411
an imporved bile duct t-cannula for studies on fasciola hepatica. 1975240532
[the hatching of the eggs of the liver fluke fasciola hepatica l. under various co2-concentrations (author's transl)].the presupposition for the hatching of the eggs of fasciola hepatica is the presence of undissociated co2 with the most effective concentration of 2-10 mmols/l. higher concentrations show inhibitory effects (abb. 6). in hatching ph has only importance in dissociation of carbonic acid and so in maintaining free co2. to provide for sufficient co2 at higher ph - but below 8.3 - the concentration of carbonic acid must be increased by higher concentrations of metallic ions electrically neutralized by ...1975242135
attempts to immunize rats against infection with fasciola hepatica using in vitro culture antigens from newly excysted metacercariae.attempts were made to immunise rats against fasciola hepatica using the culture products obtained from the in vitro cultivation of newly excysted metacercariae. three culture regimes were chosen: (1) medium nctc 135 for 48 h (2) nctc 135 + 20 per cent fetal calf serum (fcs) for 48 h (3) nctc 135 + 20 per cent fcs for 14 days. the used culture medium from each of these regimes was concentrated, mixed with adjuvant and injected subcutaneously into rats. similarly treated unused culture media was u ...1979262614
[diagnostic and therapeutic difficulties in a case of liver fluke infestation in a 4-year-old child]. 1977267892
[fasciola hepatica distomatosis in uruguay. i. experimental infections. various epidemiological, physiopathological and immunological aspects]. 1977279068
[fascioliasis in puerto rico: report of a case]. 1978280336
the anthelmintic activity of some benzimidazole derivatives against fasciola hepatica. 1978281659
the development of an experimental vaccine against fasciola hepatica in cattle. 1978287115
[fasciolosis in mexico. present status and intermediary hosts]. 1978288113
effects of the injectable contraceptive depot medroxyprogesterone acetate in thai women with liver fluke infestation: results after six months.the effect of the three-monthly injectable contraceptive depot medroxyprogesterone acetate (dmpa) on liver and lipid function was assessed in thai women with liver fluke (opisthorchis viverrini) infestation, dmpa administration being started in the immediate postpartum period. immediate postpartum iud and sterilization acceptors with fluke infestation were recruited as a comparison (control) group for the fluke-positiv dmpa acceptors. comparable groups of fluke-negative acceptors were recruited ...1977302157
anthelmintic efficacy of albendazole in cattle: comparison of critical and controlled tests.twelve calves were experimentally inoculated with 6 species of gastrointestinal nematodes and the liver fluke, fasciola hepatica. six calves were administered albendazole at 10 mg/kg of body weight, and the drug efficacy was determined by critical and controlled tests. albendazole was only 74% effective against haemonchus contortus (cambendazole-resistant strain), 99 to 100% effective against ostertagia ostertagi, trichostrongylus colubriformis, cooperia oncophora, and oesophagostomum radiatum, ...1977334003
[comparative studies on the serodiagnosis of bovine fasciolosis using the indirect immunofluorescence and immunoperoxidase test (elisa)]. 1977336033
integrated control of trematode diseases. 1977343529
[a practical system for cultures of fasciola hepatica eggs (author's transl)]. 1977346025
[immunological diagnosis of fasciola hepatica distomatosis. (apropos of 105 cases)]. 1978353413
microplate enzyme linked immunosorbent assay for antibody to fasciola hepatica in cattle. 1978358547
the regulation of respiratory metabolism in parasitic helminths. 1978364957
[determination of cholinesterase activity in fasciola hepatica homogenates].the cholinesterase activity in fasciola hepatica homogenates was studied through biological techniques. results depict that the contractile stimulating action of a constant acetylcholine dose on the isolated rat duodenum is withdrawn when the agent is previously incubated at 37 degrees c during 30 minutes with different dilutions (20%, 40% and 80%) of fasciola hepatica homogenates. the action is recovered when an anticholinesterase, as neostigmine is previously added to the homogenate. since the ...1978368914
the identification of soluble adult antigen on the tegumental surface of juvenile fasciola hepatica.an antiserum was raised in rabbits against a soluble extract of fresh homogenized adult fasciola hepatica of rat origin and was then absorbed with rat and mouse tissue antigens. this antiserum reacted specifically with the surface coat of adult flukes, of both rat and mouse origin, by indirect immunofluorescence to show the detail of surface spines. when tested against juvenile stages recovered from mice the reaction was positive with all but the earliest hepatic parenchyma stages. no reaction w ...1978372892
[effect of helminth-trematode extracts and liver implants on hepatocyte mitotic activity]. 1979385267
use of immunologic techniques to detect chemotherapeutic success in infections with fasciola hepatica. ii. the enzyme linked immunosorbent assay in infected rats and rabbits.an enzyme-linked immunosorbent assay (elisa) was developed using microtiter plates for the immunodiagnosis of fascioliasis in rats and rabbits using extracts of adult worms partially purified by gel filtration chromatography using sephacryl s-200. partial purification was necessary to eliminate cross-reactivity with antisera having antibodies to schistosomes. soft polyvinyl plates clearly gave superior results over hard polystyrine plates. titers rose by 4 weeks of infection in rats with fasciol ...1979390109
[detection of blood group p1 activity in f. hepatica and f. gigantica (author's transl)].four lyophilizated liver flüke antigens and one of hydatid fluid were examined for the presence of blood group like antigens p1, a, b, m and n by an hemagglutination inhibition test with hyperimmune and natural hemagglutinating sera. p1 or p1-like substance was found in the extracts of "fasciola hepatica" and "f. gigantica" in a lower concentration than in echinoccocal fluid. this presence may explain the occurrence of anti-p1 antibodies in patients infected with liver flüke and belonging to the ...1979392420
the ultrastructural characterization of the tegument of clonorchis sinensis (cobbold, 1875) cercaria.the tegumental ultrastructure of the cercaria of the liver fluke, clonorchis sinensis (cobbold, 1875), was studied by scanning and transmission electron microscopy. the body surface is almost encircled by many rows of regularly arranged spines. the tegumental syncytium of the body contains many rod-shaped dense granules and central electron-lucent bodies, neither of which are present in the tail tegument. there are four rows of hooked teeth and modified spines on the oral cone. these teeth are d ...1979394497
[fasciola hepatica distomatosis in maghreb. 2 new algerian cases].a bibliographic survey is made of the cases of fasciolasis in maghreb, and it can be concluded that: the notion of "epidemy", or better, of simultaneous cases, brought to the diagnosis evocation, to explorations by i.d.r. and to early treatment, before the excretion of eggs. due to drug-efficiency, the coprological proof of the disease was generally missing in the old "epidemies" of the maghreb. isolated cases, in contrast, where the fasciolasic etiology was not evocated, could follow natural ev ...1979397806
experimental infection of cats with the liver fluke platynosomum concinnum,.the severity of platynosomum concinnum infection in cats experimentally infected with 125 flukes (small dose) and 1,000 flukes (large dose) was determined by monitoring clinical signs and hematologic, serum biochemical, and pathologic changes during the infection. all cats (8/8) with a small fluke burden and 3 of 8 cats with a large burden remained clinically asymptomatic, whereas 60% of the cats given large doses had mild signs of inappetence and lethargy. eosinophilia, peaking 4 to 5 months af ...1977402094
[buxtonella sulcata, an intestinal ciliate of apparently frequent occurrence in danish cattle (author's transl)].faeces samples from 762 apparently healthy heifers were examined for eggs of fasciola hepatica (sedimentation test). at the same time possible ciliate cysts were recorded. such cysts, usually circular, and varying in size from ab. 55 micrometer to ab. 95 micrometer (average ab. 71 micrometer) were found in 71.8 % of the samples. according to morphological descriptions in literature, the cysts observed are supposed mainly to represent the species buxtonella sulcata. their presence in faeces sampl ...1977411113
phenol oxidase activity: inductionin female schistosomes by in vitro incubation.a biochemical methods has been developed for detecting phenol oxidase in female schistosoma mansoni. enzyme activity is observed only after incubation of the female schistosomes for an extended period of time in tissue culture media. male s. mansoni do not contain detectable levels of phenol oxidase activity. the properties of this enzyme are similar to those identified for a phenol oxidase from fasciola hepatica. l-dopa, dopamine, and tyrosine were found to be good substrates for this enzyme. v ...1978417162
laurell crossed immunoelectrophoresis and affinity chromatography for the purification of a parasite antigen.laurell crossed immunoelectrophoresis (two-dimensional electroimmunodiffusion) was used to prepare minute amounts of purified parasite antigens complexed with their precipitating antibodies obtained from rabbits. these complexes, emuslified in freund's complete adjuvant, were then used to prime rabbits for selective production of precipitins to the complexed antigens when the animals were later boosted with whole parasite extract. the igg antibody from the monospecific antiserum recovered was th ...1978418120
the formation of propionate and acetate as terminal processes in the energy metabolism of the adult liver fluke fasciola hepatica. 1979421954
[fasciola hepatica in denmark. a survey on 15 years diagnostic examination on bovine faeces samples (author's transl)].the results of routine diagnostic examinations of 10,540 bovine faeces samples for eggs of fasciola hepatica during the period 1963-1977 are evaluated. 0n an average, 10.4% of the samples were positive. the frequency of positive samples was highest among samples from cows (14.4%) lowest among samples from calves (1.5%). of samples from heifers 10.1% were found positive. calculated on a monthly basis, the percentage of positive samples showed a clear seasonal variation, reaching a peak in the per ...1979424278
perilobular fibrosis in chronic bovine fascioliasis.the character, extent, and localization of perilobular fibrosis were studied in cattle experimentally infected with fasciola hepatica. such fibrosis is indirectly due to the liver flukes. the regionally localized fibrosis results from cholestasis due to transient occlusion of branches of the biliary tree. in periobular fibrosis it is possible to distinguish a florid, an intermediate, and an uncomplicated form. the florid phase is marked by proliferation of the biliary epithelium and is followed ...1979432878
attempts to immunise rats and mice against infection with fasciola hepatica using antigens prepared from taenia hydatigena.attempts were made to immunise rats and mice against infection with f. hepatica by oral dosing with t. hydatigena eggs, or by vaccination with various t. hydatigena antigen preparations. these antigens included extracts from t. hydatigena cysticerci and cyst fluid, and antigens collected during short-term (48 h) and long-term (14 days) in vitro cultivation of larvae. immunity was assessed by the numbers of f. hepatica recovered from the challenge infection in rats, and the mortality rates of inf ...1979433385
the longevity of fasciola hepatica metacercariae encysted on herbage.an experiment was described in which guinea pigs were used as tracer animals in an attempt to assess the longevity of fasciola hepatic metacercariae on pasture at werribee, victoria. the metacercariae had been encysted on both an irrigated and non-irrigated area at various times during 1976. it was determined that, under the conditions of the experiment, metacercariae can survive and remain viable for up to 10 weeks during the winter. it was also determined that longevity during the summer was g ...1979444161
the effect of prior infection with fasciola hepatica on the resistance of sheep to the same parasite.the development of flukes that resulted from a challenge dose of 175 f. hepatica metacercariae was compared in 4 groups of sheep that were maintained under grazing conditions on fluke-free pasture. one group had been previously uninfected and all subgroups of each of the other 3 groups had been exposed to one of a range of previous doses of metacercariae. the preliminary infections of 2 of the 3 groups had been terminated respectively after 7 and 14 weeks. the third previously infected group had ...1979444162
the fasciolicidal activity of a halogenated benzenesulfonanilide.halogenated benzenesulfonanilides have potent fasciolicidal activity. the efficacy of 1 such compound in mice, sheep, and cattle is reported.1979446603
the hypoalbuminaemia of ovine fascioliasis: the influence of protein intake on the albumin metabolism of infected and of pair-fed control sheep. 1979447441
fasciola hepatica: some properties of a precipitate which forms when metacercariae are cultured in immune rat serum. 1979447443
fascioliasis: nitrogen balance studies and the disposition of excessive proline in rats.nitrogen balance studies were conducted and the results compared between rats infected with fasciola hepatica and uninfected rats as follows: rats with 90 to 100 day infections were randomly selected and then matched by weight to uninfected controls. all rats were then fed a 6% protein diet for 3 days. nitrogen balance data indicated that infected rats excreted significantly higher urinary nitrogen than the healthy ones. as large quantities of proline were known to be released into the host in f ...1979448471
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