| barcoding ciliates: a comprehensive study of 75 isolates of the genus tetrahymena. | the mitochondrial cytochrome-c oxidase subunit 1 (cox1) gene has been proposed as a dna barcode to identify animal species. to test the applicability of the cox1 gene in identifying ciliates, 75 isolates of the genus tetrahymena and three non-tetrahymena ciliates that are close relatives of tetrahymena, colpidium campylum, colpidium colpoda and glaucoma chattoni, were selected. all tetrahymenines of unproblematic species could be identified to the species level using 689 bp of the cox1 sequence, ... | 2007 | 17911319 |
| passage through tetrahymena tropicalis triggers a rapid morphological differentiation in legionella pneumophila. | the intracellular bacterial pathogen legionella pneumophila follows a developmental cycle in which replicative forms (rfs) differentiate into infectious stationary-phase forms (spfs) in vitro and in vivo into highly infectious mature intracellular forms (mifs). the potential relationships between spfs and mifs remain uncharacterized. previously we determined that l. pneumophila survives, but does not replicate, while it transiently resides (for 1 to 2 h) in food vacuoles of the freshwater ciliat ... | 2008 | 18805971 |
| Passage through Tetrahymena tropicalis enhances the resistance to stress and the infectivity of Legionella pneumophila. | Legionella pneumophila is a gram-negative bacterium prevalent in fresh water which accidentally infects humans and is responsible for the disease called legionellosis. Intracellular growth of L. pneumophila in Tetrahymena is inconsistent; in the species Tetrahymena tropicalis stationary-phase forms (SPFs) of L. pneumophila differentiate into mature intracellular forms (MIFs) without apparent bacterial replication and are expelled from the ciliate as pellets containing numerous MIFS. In the prese ... | 2011 | 22092856 |
| efficient expression of truncated recombinant cadmium-metallothionein gene of a ciliate, tetrahymena tropicalis lahorensis in escherichia coli. | truncated recombinant metallothionein gst-fusion protein has been successfully expressed in escherichia coli. the previously identified novel cd-inducible metallothionein (tmcd1) gene from the locally isolated ciliate, tetrahymena tropicalis lahorensis, was inserted into a pet-41a vector, in frame with a sequence encoding an n-terminal glutathione-s-transferase (gst) tail. truncated recombinant gst fusion protein has been purified by affinity column chromatography using glutathione sepharose. af ... | 2013 | 24197693 |
| species of tetrahymena identical by small subunit rrna gene sequences are discriminated by mitochondrial cytochrome c oxidase i gene sequences. | the mitochondrial cytochrome c oxidase 1 (co1) genes of two isolates of each of the seven mating types of tetrahymena thermophila were sequenced and found to differ by < 1% in nucleotide sequence and to be identical by putative protein sequence. as this gene was highly conserved in this species, the co1 gene sequence was determined for four pairs of tetrahymena species identical in their small subunit rrna gene sequences. the following pairs of species showed from 1% to 12% divergence at the nuc ... | 2006 | 16968457 |
| barcoding tetrahymena: discriminating species and identifying unknowns using the cytochrome c oxidase subunit i (cox-1) barcode. | dna barcoding using the mitochondrial cytochromecoxidase subunit i (cox-1) gene has recently gained popularity as a tool for species identification of a variety of taxa. the primary objective of our research was to explore the efficacy of using cox-1 barcoding for species identification within the genustetrahymena. we first increased intraspecific sampling fortetrahymena canadensis, tetrahymena hegewischi, tetrahymena pyriformis, tetrahymena rostrata, tetrahymena thermophila, and tetrahymena tro ... | 2011 | 20708435 |
| identification, cloning and sequencing of a novel stress inducible metallothionein gene from locally isolated tetrahymena tropicalis lahorensis. | a novel cadmium inducible metallothionein (tmcd1) gene has been identified and sequenced from the locally isolated ciliate, tetrahymena tropicalis lahorensis from industrial effluents. the tmcd1 gene encodes 471 nucleotides, with tga as the stop codon and taa coding for glutamine. this new gene is quite different from the previously reported mt genes in tetrahymena pyriformis and tetrahymena pigmentosa. however, it shows 78% homology with four different cd-mt genes reported from tetrahymena ther ... | 2007 | 17949926 |
| isolation and characterization of a novel copper-inducible metallothionein gene of a ciliate, tetrahymena tropicalis lahorensis. | the two isoforms of copper metallothionein (cumt) gene of a copper resistant ciliate, tetrahymena tropicalis lahorensis (ttl), have been isolated and characterized. the molecular cloning and nucleotide sequencing of cdnas coding for the two cumt isoforms revealed that ttlcumt1 gene has 300, while ttlcumt2 has 327 nucleotides, both with atg as the initiation codon and tga as the translational termination codon. tag codes for glutamine in ttlcumt2 gene which is peculiar to tetrahymena. the deduced ... | 2010 | 20512924 |