| two-dimensional agarose gel electrophoresis as a tool to isolate genus- and species-specific repetitive dna sequences. | two-dimensional electrophoresis in agarose gels separates dna-restriction fragments not only by molecular weight but also according to their at-cluster content. the method produced genus-specific spot patterns of multicopy dna fragments of grains as well as spot patterns of highly repetitive dna fragments of ciliates, demonstrated for barley, spelt, and tetrahymena. further investigations in regard to their specificity by hybridization with three other grain species (wheat, oat, and rye) and thr ... | 2000 | 10933849 |
| barcoding ciliates: a comprehensive study of 75 isolates of the genus tetrahymena. | the mitochondrial cytochrome-c oxidase subunit 1 (cox1) gene has been proposed as a dna barcode to identify animal species. to test the applicability of the cox1 gene in identifying ciliates, 75 isolates of the genus tetrahymena and three non-tetrahymena ciliates that are close relatives of tetrahymena, colpidium campylum, colpidium colpoda and glaucoma chattoni, were selected. all tetrahymenines of unproblematic species could be identified to the species level using 689 bp of the cox1 sequence, ... | 2007 | 17911319 |
| molecular and functional evolution of tetrahymena metallothioneins: new insights into the gene family of tetrahymena thermophila. | a new metallothionein (mtt-5) gene isoform has been cloned and characterized from the ciliate tetrahymena thermophila. its amino acid sequence shows only limited similarity with other tetrahymena mts. to investigate the transcriptional activity of this gene toward heavy metals (cd, cu, zn), mrna levels were evaluated by real-time quantitative pcr. results show that the three metals induce different mtt-5-mrna levels, cd treatment eliciting the most effective induction in the first 30 min. phylog ... | 2007 | 17208053 |
| selenocysteyl-trna occurs in the diatom thalassiosira and in the ciliate tetrahymena. | selenocysteyl-trnas that decode uga were identified previously in animal and bacterial cells and the genes for these trnas have been shown to be widespread in animals and eubacteria. in the present study, we identify a selenocysteyl-trna that codes for uga in thalassiosira pseudonana, which is a diatom, and in tetrahymena borealis, which is a ciliate. the fact that these very diverse unicellular organisms also contain a selenocysteyl-trna suggests that selenocysteine-containing proteins and the ... | 1991 | 1835508 |