| serological response to the indirect fluorescent antibody test of cattle infected with theileria sergenti. | | 1976 | 769916 |
| [role of antibody detected by the indirect fluorescent antibody test in the clinical signs of cattle infected with theileria sergenti]. | | 1975 | 1104958 |
| genomic analysis of theileria sergenti stocks in japan with dna probes. | restriction fragment length polymorphisms of theileria sergenti dna from 18 different infections of cattle in 14 locations in japan were analyzed by southern blotting using t. sergenti genomic dna fragments as probes. probe pts 2 hybridized with four fragments in bamhi digested piroplasm dna, at 8.0, 7.3, 6.0 and 3.4 kb. probe pts 11-d1 hybridized with multiple fragments. with each probe, polymorphisms were observed among stocks from different locations. however, there was no correlation between ... | 1992 | 1348593 |
| molecular cloning and immunological analysis of immunodominant piroplasm surface proteins of theileria sergenti and t. buffeli. | cdna libraries of theileria sergenti and t. buffeli piroplasms were constructed in lambda gt11 and screened with rabbit anti-piroplasm sera. a major antigen of t. sergenti (33 kda) and that of t. buffeli (34 kda) was identified from the recombinant phages by using recombinant antigen-selected monospecific antibodies. the reactivities of the cloned proteins with rabbit antisera, infected calf sera and mouse monoclonal antibody suggested that the 33 and 34 kda proteins expressed species-common and ... | 1992 | 1376625 |
| incorporation of [3h]hypoxanthine by short-term cultured theileria sergenti and its inhibition by drugs. | red blood cells parasitized with theileria sergenti were cultured in vitro for a short period in microplate wells. addition of [3h]hypoxanthine to cultures enabled us to titrate the intraerythrocytic parasite growth because a significant amount of [3h]hypoxanthine was incorporated into the parasitized red blood cells (prbc) in proportion to the number of prbc. the incorporation of [3h]hypoxanthine was almost completed in an early phase of incubation. bovine peripheral blood leukocytes incubated ... | 1992 | 1403428 |
| immunogenicity and protective efficacy of solubilized merozoite-enriched theileria sergenti immunogens. ii: protection against natural exposure under field conditions. | a theileria sergenti soluble merozoite preparation containing the 29, 34, 35 and 105 kd as the immunodominant polypeptides, was evaluated for efficacy, safety and protectivity in holstein calves against virulent field tick challenge. the soluble antigens (100 mg/dose) were fortified with either complete or incomplete freund's adjuvant. twenty naive calves, aged one month, were subcutaneously inoculated with the preparation and a booster dose was administered 4 weeks later. twenty additional calv ... | 1992 | 1420033 |
| detection and characterization of theileria sergenti proteinases. | the lysate of theileria sergenti piroplasms was tested for proteinases using sodium dodecyl sulfate-polyacrylamide gel electrophoresis in which substrate was included in gel matrix. six proteinases of molecular weight 330, 125, 98, 94, 67 and 58 kilodalton (kda) were detected. from the results of the triton x-114 phase partition, 330, 125 and 58 kda proteinases were partitioned into aqueous phase, which indicated that they were not associated with parasite membranes. all these three enzymes were ... | 1992 | 1420575 |
| analysis of the genes encoding immunodominant piroplasm surface proteins of theileria sergenti and theileria buffeli by nucleotide sequencing and polymerase chain reaction. | the nucleotide sequences of the cdnas encoding a 33-kda piroplasm protein of theileria sergenti (p33) and a similar protein of theileria buffeli (p34) were determined. both of the genes contained an open reading frame of 849 base pairs. the predicted amino acid sequence of p33 and p34, consisting of 283 residues, showed 82% similarity. a transmembrane hydrophobic domain and signal peptides were predicted. the polymerase chain reaction was used to amplify p33/34 genes from the piroplasm dna of t. ... | 1992 | 1474995 |
| antigenic differences between japanese theileria sergenti and other benign theileria species of cattle from australia (t. buffeli) and britain (t. orientalis). | serological comparisons among piroplasm antigens of the benign theileria species of cattle from japan, australia and britain, which are frequently referred to as t. sergenti, t. buffeli and t. orientalis, were carried out. the results obtained from comparative enzyme-liked immunosorbent assay (elisa) using sera from infected cattle suggest that t. sergenti could be differentiated from both t. buffeli and t. orientalis by their serological dissimilarities. western blotting combined with two-dimen ... | 1992 | 1557325 |
| plasma high density lipoprotein in anemic cattle infected with theileria sergenti. | changes in plasma concentration of lipid composition were analysed in cattle with anemia due to theileria sergenti infection. plasma levels of phospholipids, cholesterol, free cholesterol, high density lipoprotein cholesterol, and vitamin e decreased to 40-67% of the pre-infection levels, corresponding to the decrease of pcv due to the infection. however, no definite changes were detected in plasma level of triglyceride. by gradient centrifugation, it was confirmed that lipid components, other t ... | 1992 | 1558876 |
| preliminary biochemical characterization of 'veil' structure purified from theileria sergenti-, t. buffeli- and t. orientalis-infected bovine erythrocytes. | a structure called 'veil' in theileria sergenti-, t. buffeli- or t. orientalis-infected bovine erythrocytes was purified from erythrocyte lysates by percoll density-gradient centrifugation. on electron microscopical examination, the veils consisted of electron-dense material showing a periodicity of striations and were not surrounded by membranes. analyses of veil proteins by one- and two-dimensional polyacrylamide gel electrophoresis revealed that the veils contain haemoglobin and several other ... | 1992 | 1594288 |
| theileria sergenti infection in dairy cattle. | | 1992 | 1606269 |
| immunogenicity and protective efficacy of solubilized merozoite-enriched theileria sergenti immunogens. i: protection against homologous stabilate challenge. | theileria sergenti were isolated from infected erythrocytes by hypotonic lysis, and soluble merozoite antigens were purified by sonication and differential centrifugation. the preparation contained 29, 34, 35 and 105 kd immuno-dominant poly-peptides. the soluble antigens (0.5 mg/ml) were prepared and fortified with freund's adjuvant. five month old naive korean calves were subcutaneously inoculated with the preparation and a booster dose was administered 4 weeks later. nine weeks after the boost ... | 1992 | 1627501 |
| theileria sergenti proliferates in scid mice with bovine erythrocyte transfusion. | the unavailability of in vitro or in vivo experimental systems has been the major factor hampering the progress of research studies on theileria sergenti, causative agent of theileriosis, a major disease of cattle in japan. we report the first successful propagation of t. sergenti in scid mice into which uninfected bovine erythrocytes (bo-rbc) were supplied periodically. the infectivity of t. sergenti proliferated in an scid mouse was ascertained by successful transfer of infection into another ... | 1992 | 1635038 |
| activation of bovine peripheral blood monocyte and its suppressive effect on parasitemia in theileria sergenti infected calves. | activation of bovine peripheral blood monocytes and its suppressive effect on parasite growth was examined in theileria sergenti-infected calves by using a rosette assay that detects changes in fc receptor expression and by luminol-dependent chemiluminescence response. monocyte activation preceded the peak of parasitemia but was depressed parallelly with the growth of the parasites. when four calves were treated with prednisolone, three showed a good correlation between the suppression of monocy ... | 1992 | 1643171 |
| rebound phenomenon of parasitemia in splenectomized calves primarily infected with theileria sergenti. | | 1991 | 1830765 |
| proliferation of lymphocytes in theileria sergenti-infected calves in vitro. | | 1991 | 1830773 |
| changes in the proportion and number of monocytes in the peripheral blood of calves infected with theileria sergenti. | | 1991 | 1830809 |
| decrease in erythrocyte survival in theileria sergenti-infected calves determined by non-radioactive chromium labelling method. | pathogenesis of anemia in the calves infected with theileria sergenti was investigated from the viewpoint of erythrocyte survival decrease in the circulating blood. for investigation of erythrocyte survival a method of erythrocyte labelling with non-radioactive chromium (50cr) was utilized. it was found that (1) the erythrocyte survival decreased markedly in the t. sergenti-infected calves compared with that in the uninfected calves; the survival rate of 25.7% for infected calves and 86.0% for u ... | 1991 | 1832027 |
| immunohistological staining for detection of theileria sergenti schizonts. | | 1991 | 1834220 |
| induction of anti-theileria sergenti antibodies in calves with murine monoclonal anti-idiotype antibody. | anti-idiotype (anti-id) antibody that contains an internal image of theileria sergenti (t. sergenti) merozoite surface antigen was intramuscularly inoculated to calves to induce anti-t. sergenti antibody. in immunized calves, anti-anti-id antibodies were induced and these antibodies bound to merozoites of t. sergenti were confirmed by immunofluorescence assay (ifa) and enzyme-linked immunosorbent assay (elisa). in this experiment, one calf with the highest antibody titer against anti-id antibody ... | 1991 | 1836363 |
| evaluation of high sensitive dna probe for the detection of theileria sergenti infection in cattle. | | 1991 | 1836384 |
| purification of merozoites of theileria sergenti from infected bovine erythrocytes. | a simple and rapid method for purifying merozoites of theileria sergenti from infected bovine erythrocytes was developed. infected erythrocytes were lysed by the use of a cytolytic toxin produced by aeromonas hydrophila and the lysate was subjected to ultracentrifugation in a percoll discontinuous density gradient. pure and morphologically intact merozoites free of erythrocyte membrane were recovered from a band formed at the interface of 40% and 60% (by vol.) percoll solutions. in this merozoit ... | 1991 | 1902954 |
| activation of bovine peripheral blood macrophages in theileria sergenti-infected calves. | macrophage activation in theileria sergenti-infected calves was studied by testing the production of oxygen metabolites in macrophages following specific and non-specific stimulation with t sergenti merozoites or zymosan, respectively. six calves were inoculated with merozoites and three calves with sporozoites. all showed significant macrophage activation within one month after inoculation (p less than 0.05). activation of macrophages appeared earlier than parasitaemia or the peak of antibody t ... | 1991 | 1904619 |
| protein analysis of theileria sergenti/buffeli/orientalis piroplasms by two-dimensional polyacrylamide gel electrophoresis. | proteins of the piroplasms of theileria sergenti, t. buffeli and t. orientalis were analysed by two-dimensional polyacrylamide gel electrophoresis. protein spot patterns of t. buffeli and t. orientalis were identical except for a few minor proteins, whereas spot patterns of two t. sergenti stocks were differentiated from those of t. buffeli and t. orientalis by a characteristic set of proteins including a major protein of molecular weight 33-34 kda. this result indicates that japanese t. sergent ... | 1991 | 1907727 |
| responses of peripheral blood mononuclear cells in calves infected with theileria sergenti. | the role of peripheral blood mononuclear cell (pbmc) in theileria sergenti-infected calves was studied by various in vitro assay systems. proliferation of t cells in mixed lymphocyte protozoa culture (mlpc) increased with parasitemia, and the addition of monoclonal antibodies against t. sergenti merozoites in this mlpc enhanced the response. however, the addition of antibody-positive autologous serum resulted in the suppression of the response. cell-mediated cytotoxicity of pbmc increased after ... | 1991 | 1910223 |
| infection rates of theileria sergenti in haemaphysalis longicornis ticks collected from the field in japan. | for a period of one year haemaphysalis longicornis ticks were collected in a pasture from vegetation by dragging flannel cloth and from soil samples at monthly intervals. nymphal ticks were assessed for theileria infection. salivary glands were stained with methylgreen-pyronin and examined for parasite masses. nymphal h. longicornis infected with theileria sergenti were found in all samples during 12 months including the winter. after shortening the prefed period on rabbits to 24 hours, the para ... | 1990 | 2107351 |
| maturation of theileria sergenti in salivary glands of haemaphysalis longicornis induced by temperature stimulation. | the parthenogenetic haemaphysalis longicornis larvae engorged on cattle naturally infected with theileria sergenti were reared at 24 degrees c. the resultant nymphal ticks were incubated at 37 degrees c to clear the effect of incubation on the development and maturation of sporozoites. the sporozoites in the salivary glands of the nymphal ticks exposed to 37 degrees c for 16 days were observed by the methyl green pyronin staining method. the ticks exposed to 37 degrees c were ground up in a mort ... | 1990 | 2117090 |
| detection of the schizont stage in experimentally infected cattle with japanese theileria sergenti. | calves infected with a sporozoite suspension of the ikeda strain of theileria sergenti were examined in attempts to detect the schizont stage. a suspension equivalent to the contents of 70-200 ticks was inoculated subcutaneously into 10 splenectomized calves. schizonts and micromerozoites were found in the prescapular lymph nodes of 5 calves about 8 days after the sporozoite inoculation. schizonts detected at the time of nuclear division had large and diffuse nuclei, and many of the schizonts we ... | 1990 | 2117091 |
| protective effect against intraerythrocytic merozoites of theileria sergenti infection in calves by passive transfer of monoclonal antibody. | | 1990 | 2117092 |
| monoclonal internal image anti-idiotype antibodies of theileria sergenti merozoite surface antigen. | monoclonal anti-idiotype antibodies against monoclonal antibody (23c11) to intraerythrocytic merozoites of theileria sergenti were prepared and examined by indirect immunofluorescence assay (ifa) and enzyme-linked immunosorbent assay (elisa). three anti-idiotype antibodies containing antibodies against interspecies cross-reactive idiotopes and/or an internal image of theileria sergenti merozoite were found. the presence of antibody against theileria sergenti was shown by elisa in these anti-idio ... | 1990 | 2118200 |
| specific dna probe for the detection of theileria sergenti infection in cattle. | a simple procedure was developed for detection of theileria sergenti infection on the basis of hybridization of parasite dna with a specific probe. a genomic dna library of t. sergenti constructed in puc-18 was screened to detect clones containing the parasite's dna sequences by colony and southern hybridizations. two positive dna inserts were purified from the recombinant plasmids and used as probes labelled with 32p or non-isotopic reagent, biotin-11-dutp. 32p-radiolabelled and non-radioactive ... | 1990 | 2126824 |
| intraerythrocytic schizogony of theileria sergenti in cattle. | the characteristics of developing intraerythrocytic stages of t. sergenti were studied by light and transmission electron microscopy. the parasites with many ribosomes, acristate mitochondria, cytostome, and food vacuoles were morphologically regarded as the trophozoite stage. although this type of parasites was frequently detected, intraerythrocytic merozoite stage with electron dense cisternae, rhoptries and small electron dense bodies was rarely observed in high parasitaemia. the intraerythro ... | 1990 | 2126825 |
| invasion of bovine erythrocytes by theileria sergenti piroplasms in vitro. | | 1990 | 2126826 |
| opsonization of merozoites with sera from theileria sergenti-infected calves. | | 1990 | 2126827 |
| bovine monoclonal antibody specific to theileria sergenti. | | 1990 | 2126829 |
| clinico-hematological observation of calves experimentally infected with theileria sergenti. | | 1990 | 2287144 |
| development of theileria sergenti in the midgut of the larval tick, haemaphysalis longicornis. | | 1987 | 2455095 |
| localization of surface antigens on theileria sergenti merozoite by monoclonal antibodies. | | 1989 | 2511372 |
| bar-structure in bovine erythrocytes infected with theileria sergenti. | the purpose of this study is to elucidate some morphological characteristics of the bar-structure in bovine erythrocytes infected with theileria sergenti. the bar-structure, the veil or the both were observed in infected erythrocytes. infected bovine erythrocytes were classified into four types according to the included structures. infected cells containing bar-structures increased with the progress of parasitemia. in giemsa-stained blood preparations, bar-structures appeared purplish-red and me ... | 1989 | 2513447 |
| correlation between the infection rate of the vector tick, haemaphysalis longicornis and the parasitaemia of cattle infected with theileria sergenti. | the infection rate of theileria sergenti in salivary glands of nymphal haemaphysalis longicornis which had dropped from cattle showing different piroplasm parasitaemias was examined by the methyl green-pyronin staining method. the results suggested that there was some correlation between parasitaemia and the subsequent infection rate in salivary glands of ticks. it seemed possible that the parasitaemia in cattle, especially the number of parasitized erythrocytes in blood imbibed by ticks, might ... | 1989 | 2596906 |
| abnormality of osmotic fragility and morphological disorder of bovine erythrocytes infected with theileria sergenti. | the osmotic fragility and the surface structure of erythrocytes obtained from 3 calves infected with theileria sergenti and from 3 phlebotomized ones were compared. as the parasitemia progressed, the osmotic fragility of the erythrocytes significantly increased in the infected calves. particularly the hemolysis ratio in the isotonic area (21.5-94.1%) obviously increased. on the other hand, the percentage of parasitized cells in the erythrocytes did not show so much high values (16.1-21.3%). simi ... | 1989 | 2739214 |
| theileria sergenti: a simple method for isolation of piroplasms from erythrocytes. | | 1989 | 2739228 |
| intraerythrocytic ca2+ and mg2+ concentrations of theileria sergenti infected calves. | | 1989 | 2739229 |
| enhancement of antibody production against sheep erythrocytes using theileria sergenti lysate antigen in different strains of inbred mice. | | 1986 | 3090336 |
| a rise and fall of humoral antibody in calves experimentally infected with babesia ovata or theileria sergenti. | | 1986 | 3090337 |
| the development of theileria sergenti in the salivary glands of the tick, haemaphysalis longicornis. | | 1986 | 3093743 |
| development of theileria sergenti in the midgut of the tick, haemaphysalis longicornis. | | 1987 | 3108562 |
| antigenic properties of theileria sergenti in elisa serodiagnosis. | | 1987 | 3112459 |
| monoclonal antibodies against intraerythrocytic merozoites (piroplasms) of theileria sergenti. | | 1987 | 3114531 |
| development of theileria sergenti in the haemolymph of the tick, haemaphysalis longicornis. | the haemolymph of adult tick haemaphysalis longicornis infected with theileria sergenti, was examined at regulat intervals of time. as a result, kinetics could be detected in the haemolymph for the first time 25 days after the engorgement. the kinetics detected could be classified into three types on the basis of their morphological characteristics. they increased gradually in number, reaching a maximum 32 days later. then they tended to decrease in number, disappearing almost completely from th ... | 1987 | 3116780 |
| effect of constant temperatures on theileria sergenti infection in salivary glands of nymphal haemaphysalis longicornis. | | 1988 | 3133517 |
| preparation of monoclonal antibodies to theileria sergenti and their reactivity to antigens in experimentally infected cattle. | two distinct monoclonal antibodies (3-h and 11-d) were produced against theileria sergenti. these two new products, together with monoclonal antibody 1-g obtained in a previous study, were used to detect the parasites in experimentally infected cattle. during the first period of dexamethasone treatment, which was carried out to increase parasitemia in the infected cattle, the number of erythrocytes detected by 3-h, 11-d and 1-d increased in two experimentally infected calves. during the second p ... | 1988 | 3138809 |
| enhanced adjuvant effects in embryo aggregated mouse chimera injected with theileria sergenti lysate antigen. | | 1988 | 3139905 |
| isolation of theileria sergenti piroplasms from infected erythrocytes and development of an enzyme-linked immunosorbent assay for serodiagnosis of t sergenti infections. | a method of obtaining a pure suspension of theileria sergenti piroplasms from infected bovine erythrocytes was developed and the resulting parasites used as antigen in an enzyme-linked immunosorbent assay (elisa). piroplasms were freed from infected erythrocytes using the nitrogen cavitation technique and then separated from unbroken erythrocytes by centrifugation at 670 gmax. the parasites in the supernatant were then sedimented by centrifugation at 2700 gmax and the purified fraction examined ... | 1988 | 3143133 |
| the immunoglobulin response in cattle to experimental infection with theileria sergenti. | | 1988 | 3143847 |
| biochemical characterization of theileria sergenti lysate antigen on the adjuvant effect in mice. | | 1988 | 3145351 |
| experimental transmission of theileria sergenti of cattle in japan by haemaphysalis mageshimaensis. | | 1988 | 3151544 |
| immunological and preventive effects of combined inoculation of theilerial lymphokine and immunostimulant against theileria sergenti infection. | | 1988 | 3193680 |
| enzyme-linked immunosorbent assay using solubilised antigen for detection of antibodies to anaplasma marginale. | an enzyme-linked immunosorbent assay (elisa) was used to detect antibodies against anaplasma marginale. a marginale bodies were separated from parasitised erythrocytes by a modified nitrogen decompression method, sonicated, then solubilised with triton x-100 and used as the elisa antigen. in this elisa system the required amount of antigen protein was 16.2 ng for each well. in the course of experimental infections, of calves, significant antibody levels were detected by elisa and the complement ... | 1988 | 3238774 |
| glycolytic enzyme activity and intermediate concentrations in theileria sergenti-parasitized bovine erythrocytes. | | 1988 | 3386097 |
| transovular transmission of theileria sergenti in the tick, haemaphysalis longicornis. | | 1986 | 3560656 |
| separation of bovine erythrocytes infected with theileria sergenti by "percoll-conray" density gradient. | | 1987 | 3682516 |
| development of theileria sergenti in the ovary and eggs of the tick, haemaphysalis longicornis. | | 1985 | 3842145 |
| a double screening trial for the development of drugs against theileria sergenti in cattle using babesia rodhaini and plasmodium berghei in mice. | | 1985 | 3884864 |
| scanning electron microscopy of theileria sergenti. | | 1985 | 3920423 |
| ultrastructural observations of the intra-erythrocytic merozoite in theileria sergenti. | the intra-erythrocytic merozoite of theileria sergenti was 2.2 to 2.4 micron long; several mitochondria and a large nucleus lay at the anterior and posterior part of the cell, respectively, and free ribosomes were evenly scattered in the cytoplasm. a micropore located at the antero-ventral side of the protozoon was framed by the local thickening of the plasma membrane with high electron-density, nearby a large food vacuole containing erythrocytic matrix-like substances. the highly developed roug ... | 1985 | 3923596 |
| preparation of monoclonal antibodies against theileria sergenti. | | 1985 | 3934444 |
| the presence of theileria sergenti in haemaphysalis longicornis overwintering in pasture in japan. | unfed haemaphysalis longicornis ticks were collected from the soil of pasture during the winter and their salivary glands were examined by the methyl green pyronin staining method in order to detect overwintering of theileria sergenti, the causative agent of bovine theileriosis in japan. a transmission experiment was carried out using these ticks applied to a splenectomized calf. the protozoa which appeared in the peripheral blood were identified parasitologically and seroimmunologically as t. s ... | 1985 | 3936424 |
| efficacy of naphthoquinones and imidocarb dipropionate on theileria sergenti infections in splenectomized calves. | | 1985 | 4010128 |
| immune effect of toxoplasma lysate antigen (tla) on cattle against theileria sergenti infection. | | 1985 | 4094277 |
| [occurrence of theileria sergenti on the apsheron peninsula]. | | 1970 | 4251627 |
| passage of host serum components, including antibodies specific for theileria sergenti, across the digestive tract of argasid and ixodid ticks. | | 1984 | 6433818 |
| electron microscopy of theileria sergenti in bovine erythrocytes. | | 1984 | 6439931 |
| morphological studies of theileria sergenti. ii. feeding mechanism by the cytostome of theileria sergenti. | | 1983 | 6439941 |
| clinico-hematologic and serological comparison of japanese and russian strains of theileria sergenti. | the japanese and russian strains of theileria sergenti were compared clinico-hematologically and serologically. they were subjected to passage in splenectomized calves 4 approximately 8 months old by the infectation of infected ticks or the inoculation of infected blood. as a result, the average of incubation of the japanese strain was proved to be 23 days by the tick infection and 21 days by the inoculation of infected blood stored in a frozen state. the rate of detection and the average size o ... | 1980 | 6777702 |
| efficacy of imidocarb diproprionate and primaquine phosphate in the prevention of tick-borne disease in imported hereford heifers in south korea. | this paper describes the build-up of tick-borne blood parasites in a group of hereford heifers imported from new zealand to the island of jeju do, south korea. all became infected with theileria sergenti and half with babesia bigemina. an attempt was made to contain the 2 parasites by treatment with either primaquine phosphate or imidocarb diproprionate. both drugs had some prophylactic activity against t. sergenti for at least 26 days and imidocarb dipropionate eliminated b. bigemina. since t. ... | 1981 | 7292606 |
| the use of imidocarb diproprionate for the treatment of theileria sergenti infections of cattle. | imidocarb diproprionate (imdp) was used therapeutically at a dose of 1.2 mg imdp/kg of body weight against theileria sergenti infections in hereford heifers on the island of jeju-do, republic of korea. in the first experiment imdp was seen to be at least as effective in reducing t. sergenti parasitaemias as primaquine phosphate (primaquine), the drug normally used on the island. in the second and third experiments imdp was shown to reduce t. sergenti parasitaemias by about 80%. no drug is known ... | 1981 | 7295239 |
| a genetic analysis of mixed population in theileria sergenti stocks and isolates using allele-specific polymerase chain reaction. | in order to differentiate parasite populations bearing two allelic forms of p33/32, an immunodominant piroplasm surface protein of theileria sergenti, two sets of oligonucleotide primers were designed to amplify either of the two allele by the polymerase chain reaction (pcr). each set of the primers differentially amplified either of ikeda- or chitose-type p33/32 genes. by using this allele-specific pcr and restriction enzyme digestion of amplified products, parasite populations within field iso ... | 1995 | 7492646 |
| immunogenicity and protective efficacy of solubilized merozoite-enriched theileria sergenti immunogens. iii. characterization of immunodominant peptides. | immunoblot analysis utilizing bovine sera from naturally or experimentally infected with theileria sergenti were used to determine the immunodominant polypeptides of t. sergenti (korean isolate). the previously recognized major bands, 18 kda, 29 kda, 34 kda and 45 kda, were excised after electrophoresis and transfer to pvdf membrane. the individual bands were sequenced. the 34 kda polypeptide which was the most antigenic and immunogenic peptide was observed in the western blot. however, chou-fas ... | 1994 | 7517694 |
| serum from theileria sergenti-infected cattle accelerates the clearance of bovine erythrocytes in scid mice. | anemia is a major clinical sign of japanese bovine theileriosis caused by theileria sergenti. to investigate the possible factors causing anemia in cattle, we developed a clearance test for bovine erythrocytes (bo-rbc) in severe combined immunodeficient (scid) mice. clearance of bo-rbc in the scid mice was significantly accelerated when the mice were inoculated with a serum sample obtained from an infected calf during a highly parasitized phase but not when they were injected with a serum sample ... | 1995 | 7567904 |
| characterization of epitopes on a 32 kda merozoite surface protein of theileria sergenti. | epitopes on a 32 kda protein, which is an immunodominant major surface protein of theileria sergenti, recognized by anti-merozoite monoclonal antibodies were characterized. the results of a competitive binding assay between monoclonal antibodies indicated that there were at least three epitopes in this protein. the presence of repeated epitopes was suggested by using two-site enzyme-linked immunosorbent assay. the protein was partitioned into the detergent phase of triton x-114 extracts, indicat ... | 1993 | 7680447 |
| gangliosides as a possible receptor on the bovine erythrocytes for theileria sergenti. | to elucidate whether or not gangliosides on the bovine erythrocytes serve as a receptor for theileria sergenti merozoites, the reactivities of the t. sergenti piroplasms with gangliosides were studied by the liposome agglutination test. the parasites reacted weakly with i-active ganglioside containing n-acetylneuraminic acid (neuac) and strongly with i-active ganglioside containing n-glycolylneuraminic acid (neugc). however, none of the other gangliosides expressed on the bovine erythrocytes, su ... | 1995 | 7756415 |
| alteration of ganglioside composition in the erythrocytes associated with theileria sergenti infection. | the changes in ganglioside composition of bovine erythrocytes associated with theileria sergenti infection were investigated using the erythrocytes before and after the infection. the erythrocytes before infection with t. sergenti had gm3, sialosylparagloboside (spg), i-active, and i-active ganglioside as predominant gangliosides. after infection with t. sergenti merozoites, the contents of spg and i-active ganglioside were slightly less, and i-active ganglioside content was much less in the ery ... | 1994 | 7865582 |
| analyses of antigenic and genetic diversities of theileria sergenti piroplasm surface proteins. | monoclonal antibodies (moabs) against theileria sergenti fukushima stock, an intraerythrocytic protozoan parasite of cattle, were produced. the moabs reacted with 32 kda or 23 kda piroplasm surface protein (p32 or p23) in western blot analysis. using a panel of the moabs, antigenic analysis of the stocks and field isolates distributed in japan was carried out. the results revealed antigenic diversity of t. sergenti isolates, but diversity did not appear to be correlated with the geographical reg ... | 1994 | 7948375 |
| development of theileria sergenti schizonts in the lymph node of experimentally infected cattle. | schizogony of japanese theileria sergenti of cattle was studied by light and electron microscopy. schizonts were detected in the draining lymph node between 4 and 8 days after sporozoite inoculation. macroschizonts (the phase of nuclear division having invaginations) were formed 6 days after inoculation. subsequently, microschizonts (the phase of merozoite formation displaying rosette-like appearance) were observed 8 days after inoculation. multiple infections of a host cell with sporozoites wer ... | 1994 | 7999897 |
| changes in the hybridization patterns of populations of theileria sergenti during infection. | restriction fragment length polymorphisms (rflps) of theileria sergenti dna were analysed using probes of a genomic dna fragment (pts 2) and a cdna corresponding to this genomic probe (c-ts 2). each of the probes detected rflps in dna from different stocks of theileria sergenti. additionally, using these probes, alterations in hybridization patterns were observed in samples of the parasites harvested at different times after individual calves had been infected with theileria sergenti. this resul ... | 1993 | 8101404 |
| molecular cloning and sequencing of hsp70 cdna of theileria sergenti. | | 1993 | 8139624 |
| detection of theileria sergenti infection in cattle by polymerase chain reaction amplification of parasite-specific dna. | a pair of synthetic oligonucleotide primers, designed from the gene encoding a 32-kda intraerythrocytic piroplasm surface protein of theileria sergenti, were used to amplify parasite dna from the blood of t. sergenti-infected cattle by means of the polymerase chain reaction (pcr). pcr-amplified dna was examined by electrophoresis and by dot blot or microplate hybridization using a parasite-specific cdna probe. pcr was specific for t. sergenti, since no amplification was detected with dna from an ... | 1993 | 8253950 |
| analysis of mixed parasite populations of theileria sergenti using cdna probes encoding a major piroplasm surface protein. | the gene for the 32 kda surface protein (p32) of theileria sergenti was cloned into lambda gt11 and its nucleotide sequence was determined. the gene encodes a protein of 283 amino acids as deduced from its nucleotide sequence with a 22 residue n-terminal signal peptide. using this cdna as a probe we have isolated another two clones from a cdna library with a cdm8 vector system derived from the same parasite stock. comparison with three cdna clones revealed differential polyadenylation and differ ... | 1993 | 8278218 |
| light and electron-microscopical observations of the early developmental stages of theileria sergenti in the gut of haemaphysalis longicornis. | morphological characteristics of the early developmental stages of theileria sergenti in the vector tick were observed by light and electron-microscopies and compared with those of other theileria species. just before the repletion of nymphs, various stages of the parasite were observed in gut contents of tick by light microscopy: the spherical intra-erythrocytic stage, the ring-form stage, the spindle-shaped stage and the round-form stage. electron microscopy revealed that the spherical intra-e ... | 1993 | 8286540 |
| theileria sergenti: transformation of zygotes into kinetes in vector ticks, haemaphysalis (kaiseriana) longicornis and h. (k.) mageshimaensis. | invagination or transformation of a zygote of theileria sergenti into a kinete was examined with giemsa-stained smears of the midgut of vector ticks, haemaphysalis longicornis and h. mageshimaensis. zygotes invaginated almost simultaneously with the moulting of host ticks. they were round and oval, and measured 5.4 to 6.7 microns in diameter on average. the kinetes developing in the zygotes were situated in the periphery of cytoplasm and were spindle- or club-shaped. | 1993 | 8286543 |
| development of theileria sergenti in vector ticks, haemaphysalis longicornis, during blood sucking. | | 1993 | 8346995 |
| histological observations on the schizonts in cattle infected with japanese theileria sergenti. | histological observations were performed on the schizonts of japanese theileria sergenti in three calves necropsied 8 and 10 days after application of nymphal haemaphysalis longicornis infected with t. sergenti. in all the three calves, schizonts were observed in the cytoplasm of huge cells formed in the drainage lymph nodes, liver and spleen. the huge cells were 50 to 200 microns in diameter. schizonts had granular appearance and an irregular shape and were 1 to 7 microns in diameter. ultrastru ... | 1993 | 8399735 |
| theileria sergenti infection in the bo-rbc-scid mouse model. | we have previously developed the bo-rbc-scid mouse model for theileria sergenti infection. in the present study, this model was further examined to delineate the models of parasite infection. the bo-rbc-scid mice were prepared by periodically transfusing uninfected bovine erythrocytes (bo-rbcs) into splenectomized scid mice via the intraperitoneal (i.p.) route. the mice, separated into three groups, were inoculated i.p., intravenously (i.v.), or subcutaneously (s.c.) with t. sergenti-infected bo ... | 1993 | 8415559 |
| the bo-rbc-scid mouse model for evaluating the efficacy of anti-theilerial drugs. | we have previously developed a mouse model which allowed the proliferation of theileria sergenti in severe combined immunodeficiency (scid) mice with circulating bovine erythrocytes (bo-rbc). in the present study, this model was utilized to test the efficacy of anti-theilerial drugs. bo-rbc-scid mice were created by giving periodic transfusions of t. sergenti-free bo-rbc, and subsequently infecting with t. sergenti. three anti-protozoal compounds, pamaquine (yamanouchi pharmaceutical co. ltd), g ... | 1993 | 8468129 |
| sporogony of theileria sergenti in the salivary glands of the tick vector haemaphysalis longicornis. | sporogony of theileria sergenti in the salivary glands of the tick vector haemaphysalis longicornis was observed by light and transmission electron microscopy. fission bodies that developed from kinetes were observed as masses (approximately 32 x 15 microns) that occupied half of the acinous cell at 2 days after the infestation of ticks. parasites possessed an irregular nucleus of low electron density, highly electron-dense granules, and mitochondrion-like bodies in the cytoplasm. parasites deve ... | 1993 | 8469666 |
| selection of diversity at putative glycosylation sites in the immunodominant merozoite/piroplasm surface antigen of theileria parasites. | the immunodominant merozoite/piroplasm surface antigen of theileria parasites has potential as a diagnostic reagent and as a component of a sub-unit vaccine. this molecule is known to be antigenically diverse, and it is important to determine the nature and extent of this heterogeneity. in the present study nucleotide sequences, representing alleles of the gene (tams1) encoding this molecule in theileria annulata were compared to each other and to sequences of homologous genes in theileria serge ... | 1995 | 8538686 |
| theileria sergenti and t. buffeli: polymerase chain reaction-based marker system for differentiating the parasite species from infected cattle blood and infected tick salivary gland. | benign theileria species in cattle. theileria sergenti and t. buffeli, are morphologically indistinguishable. the polymerase chain reaction (pcr) was used to amplify the genes encoding the 33- and 34-kda major piroplasm antigens (p33/34) of t. sergenti and t. buffeli from cattle blood infected with these parasites and tick salivary gland infected with t. sergenti. following amplification, the p33 gene from t. sergenti and the p34 gene from t. buffeli were clearly differentiated using the restric ... | 1995 | 8542983 |
| expression of a 32 kilodalton theileria sergenti piroplasm surface protein by recombinant baculoviruses. | previous studies detected a single amino acid substitution (ala196 to gly196) between cdna clones encoding a 32 kda antigen (p32) of theileria sergenti (chitose stock) obtained from a persistently infected calf. in this study, 2 different recombinant baculoviruses (pac/p32-ala196 and pac/p32-gly196) were constructed for the expression of p32. molecular masses of the polypeptides produced in spodoptera frugiperda cells infected with the recombinant baculoviruses were the same as that of authentic ... | 1995 | 8550294 |