| analysis of the genes encoding immunodominant piroplasm surface proteins of theileria sergenti and theileria buffeli by nucleotide sequencing and polymerase chain reaction. | the nucleotide sequences of the cdnas encoding a 33-kda piroplasm protein of theileria sergenti (p33) and a similar protein of theileria buffeli (p34) were determined. both of the genes contained an open reading frame of 849 base pairs. the predicted amino acid sequence of p33 and p34, consisting of 283 residues, showed 82% similarity. a transmembrane hydrophobic domain and signal peptides were predicted. the polymerase chain reaction was used to amplify p33/34 genes from the piroplasm dna of t. ... | 1992 | 1474995 |
| the distribution and prevalence of theileria buffeli in cattle in queensland. | the distribution and prevalence of thelleria buffeli in queensland cattle were investigated using serum samples and blood films collected primarily for brucellosis surveillance and tick fever diagnosis. serums from 8654 cattle from 357 farms throughout queensland were examined by an indirect fluorescent antibody test for antibody to t buffeli. in addition, 347 peripheral blood films collected from 147 farms in south-eastern queensland were examined for piroplasms of t buffeli. the overall herd a ... | 1992 | 1586316 |
| elimination of theileria buffeli infections from cattle by concurrent treatment with primaquine phosphate and halofuginone lactate. | fifty splenectomised calves naturally infected with theileria buffeli were treated with primaquine phosphate (ici, uk) and halofuginone lactate (hoechst, australia) either separately or in combination. infections in treated calves were monitored for up to 26 weeks by examining giemsa stained peripheral blood films for piroplasms and by an immunofluorescent antibody test. when used alone neither of the drugs eliminated infection. the most successful results were obtained when two treatments of ha ... | 1990 | 2115212 |
| elimination of theileria buffeli infections from cattle by concurrent treatment with buparvaquone and primaquine phosphate. | attempts (some successful) were made to eliminate theileria buffeli infections from 23 naturally or experimentally infected, splenectomised calves. the anti-theilerial hydroxynaphthoquinone derivative buparvaquone was used either alone or in combination with primaquine phosphate. after treatment the calves were monitored for infection for up to 26 weeks. blood films were examined for piroplasms and serum antibody levels were measured using an immunofluorescence technique. buparvaquone alone fail ... | 1990 | 2115213 |
| concurrent infection with theileria buffeli caused depression of parasitaemia in babesia bovis and anaplasma centrale infections in splenectomised calves but not in b bigemina infections. | in five experiments undertaken in splenectomised calves it was found that theileria buffeli infections depressed the parasitaemias of superimposed babesia bovis and anaplasma centrale infections, but not b bigemina infections. the course of a centrale infections did not appear to be affected by a concurrent eperythrozoon teganodes infection. | 1990 | 2267426 |
| transmission of theileria buffeli to cattle by haemaphysalis bancrofti fed on artificially infected mice. | larval and nymphal haemaphysalis bancrofti became infected with theileria buffeli following the intraperitoneal inoculation of infected bovine blood into cba mice on which they were feeding. subsequent instars of fed ticks were released on calves and transmitted theileriosis at each of 10 attempts. suspensions made from ticks moulted in vitro and then incubated for 4 days at 37 degrees c were also infective for calves inoculated subcutaneously. the findings provide a convenient method for infect ... | 1989 | 2588463 |
| observations on the development of tick-transmitted theileria buffeli (syn t orientalis?) in cattle. | the development and morphology of australian theileria buffeli in cattle were studied after infection had been experimentally transmitted by the tick, haemophysalis humerosa. macroschizonts of t buffeli were demonstrated in giemsa's stained lymph node preparations for between six and 20 days following tick infestation. the presence of schizonts was confirmed by immunofluorescence with sera from known infected animals. microschizonts were seen infrequently. the schizonts and piroplasms of t buffe ... | 1988 | 3406530 |
| haemaphysalis humerosa, not h. longicornis, is the likely vector of theileria buffeli in australia. | | 1987 | 3426468 |
| the experimental transmission of theileria buffeli of cattle in australia by haemaphysalis humerosa. | | 1987 | 3579753 |
| use of in vitro culture to isolate babesia bovis from theileria buffeli, eperythrozoon wenyoni and anaplasma spp. | on nine separate occasions, babesia bovis microaerophilus stationary phase (masp) cultures were initiated with blood from calves with concurrent infections of b. bovis and theileria buffeli, eperythrozoon wenyoni or anaplasma spp. in each case b. bovis became established in culture and was maintained for 30-49 days. culture material was inoculated into susceptible splenectomised calves to test for persistence of the other organisms. no haemoparasites other than b. bovis were detected in giemsa-s ... | 1994 | 8091617 |
| selection of diversity at putative glycosylation sites in the immunodominant merozoite/piroplasm surface antigen of theileria parasites. | the immunodominant merozoite/piroplasm surface antigen of theileria parasites has potential as a diagnostic reagent and as a component of a sub-unit vaccine. this molecule is known to be antigenically diverse, and it is important to determine the nature and extent of this heterogeneity. in the present study nucleotide sequences, representing alleles of the gene (tams1) encoding this molecule in theileria annulata were compared to each other and to sequences of homologous genes in theileria serge ... | 1995 | 8538686 |
| review of australian species of theileria, with special reference to theileria buffeli of cattle. | this review describes 4 species of parasites in australia generally regarded as theileria. the physiological and epidemiological characteristics of the species that occurs in cattle are discussed. confusion still surrounds the relationship of this species with benign species of theileria in other countries. arguments are presented to support the proposal that the bovine species in australia is correctly designated theileria buffeli and that this name should apply to all the benign species of the ... | 1996 | 8815617 |
| evidence of theileria buffeli infection in cattle in southern italy. | | 1997 | 9194301 |
| increased resistance to anaplasma marginale infection in cattle chronically infected with theileria buffeli (syn. t. orientalis). | calves chronically infected with the benign haemoprotozoan parasite theileria buffeli (syn. t. orientalis) and t. buffeli-free calves were experimentally infected with virulent anaplasma marginale. the daily mean maximum parasitaemia in the t. buffeli-carrier calves was lower and delayed relative to that of the theileria-free calves. anaemia was less marked in the theileria infected calves, although this difference was not statistically significant. the susceptibility of theileria-carrier and th ... | 1997 | 9195728 |
| identical small subunit ribosomal rna gene nucleotide sequence of bovine theileria isolates (korea and japan) and theileria buffeli (marula, kenya). | small subunit ribosomal rna (ssu rrna) gene nucleotide sequences of bovine theileria isolates from korea (kls and kcb) and japan (jhs) were determined. the genes from each isolate were amplified by the polymerase chain reaction and the approximately 1.8 kb product cloned and sequenced by a modified dideoxynucleotide method. overlapping gene segments produced with a series of primers were sequenced, resulting in a complete dna sequence for both forward and reverse strands of the ssu rrna genes of ... | 1998 | 9529863 |
| development of an enzyme-linked immunosorbent assay for detection of antibodies to babesia bigemina in cattle. | monoclonal antibodies, directed against a 58-kda babesia bigemina merozoite antigen that reacted strongly with immune sera from experimentally and naturally infected cattle in western blots, were used to develop a competitive-inhibition enzyme-linked immunosorbent assay (elisa). as based on the testing of 70 antibody-positive sera from experimentally infected cattle and 166 antibody-negative sera collected in non-endemic areas of australia, the sensitivity and specificity of the elisa were 95.7% ... | 1998 | 9747938 |
| sequence analysis of the major piroplasm surface protein gene of benign bovine theileria parasites in east asia. | relatively benign theileria parasites are widespread among cattle in east asia. although the parasites are presumed to be of the theileria sergenti/theileria buffeli/theileria orientalis group, their taxonomic status and epidemiology have not been well defined. in the present study, theilerial dna samples were collected from various east asian countries, including japan, korea, taiwan, and china. dna sequences encoding a major piroplasm surface protein were amplified by polymerase chain reaction ... | 1998 | 9762568 |
| molecular phylogenetic studies on theileria parasites based on small subunit ribosomal rna gene sequences. | classification of theileria parasites of south-east asian countries is still ambiguous due to the lack of basic studies, especially their molecular genetic information. in this study, we included 6 known species and 14 unclassified theileria parasite isolates: theileria annulata, theileria parva, theileria taurotragi, theileria sergenti, theileria buffeli, theileria types sable, theileria types a, b, b1, b2, c, d, e, f, g, g1, theileria type medan (indonesia), theileria type ipoh (malaysia) and ... | 1999 | 10392966 |
| molecular cloning and characterisation of 23-kda piroplasm surface proteins of theileria sergenti and theileria buffeli. | the cdna encoding a 23-kda piroplasm membrane protein (p23) of theileria sergenti chitose (c)-type was isolated and its nucleotide sequence was determined. the gene encodes a polypeptide of 223 aa with a 28 residue n-terminal signal sequence and a hydrophobic, valine-rich, c-terminal transmembrane domain, as deduced from its nucleotide sequence. southern blot hybridisation analysis proved that p23 gene was a single copy gene and had allelic forms of the gene in the parasite population. by pcr, t ... | 1999 | 10428635 |
| phylogenetic relationships of the benign theileria species in cattle and asian buffalo based on the major piroplasm surface protein (p33/34) gene sequences. | in order to examine the taxonomic relationship of theileria sp. of asian buffalo to the benign theileria spp. of cattle, we sequenced and compared the major piroplasm protein (p33/34) genes of these parasites. the two consensus sequences determined for the buffalo parasite were of the same length (852 bp) and showed >80% identity with the sequences of the homologous genes (849 bp) in the cattle parasites. alignment of the inferred aa sequences with those of theileria sergenti and theileria buffe ... | 1999 | 10428638 |
| theileriosis in a missouri beef herd caused by theileria buffeli: case report, herd investigation, ultrastructure, phylogenetic analysis, and experimental transmission. | a 6-year-old simmental cow infected with theileria buffeli had a clinical disease characterized by theilerial parasitemia, macrocytic normochromic anemia with acanthocytosis and spherocytosis, lymphoid hyperplasia (lymphocytosis, edematous lymphadenomegaly), dysproteinemia, evidence of liver disease, and a low serum antibody titer against t. buffeli. the cow was in a herd in which all cattle originated in missouri; 22/75 (29%) of cattle had a theilerial parasitemia and 26/75 (35%) had titers to ... | 2000 | 10643976 |
| molecular characterisation of the theileria buffeli/orientalis group. | benign bovine theileria parasites known as either theileria buffeli, theileria orientalis or theileria sergenti are classified on basis of their morphology, vector specificity, pathogenicity and 18s small subunit ribosomal rna or major piroplasm protein (mpsp) sequences. since most isolates have been characterized on only some of these criteria and the existing confusion in nomenclature, an analysis was performed on eight different isolates to combine 18s rrna data with mpsp data and the results ... | 2000 | 10927085 |
| tick-borne diseases of livestock in italy: general review and results of recent studies carried out in the apulia region. | this paper reviews basic information on the knowledge of tick-borne diseases, babesiosis, anaplasmosis and theileriosis, in horses, cattle, sheep and goats in italy with particular reference to the southern part of the country. it is stressed that there is limited knowledge about the parasite species present, their vectors, distribution, prevalence and impact on livestock production and there is the need to assess their epidemiology. a study carried out in the apulia region to assess the prevale ... | 1999 | 11071537 |
| identification of theileria buffeli/orientalis and babesia bigemina in apulian cattle using molecular techniques and study of changes in blood parameters. | recently several cases of theileriosis due to the haemoprotozoan theileria buffeli/orientalis have been recorded in the apulian region, italy. in this area other tick-borne pathogens were usually identified such as anaplasma marginale and babesia bigemina. outbreaks were recorded showing that these pathogens can be observed separately or in mixed infections. sub-clinical cases and carrier animals were also previously identified. a lack of specific techniques could not rule out the presence of ot ... | 1999 | 11071538 |
| theileria buffeli infection of a michigan cow confirmed by small subunit ribosomal rna gene analysis. | theileria buffeli, generally a benign parasite of cattle, has been reported in animals from texas, missouri and north carolina. to date, there have been no reports of the parasite in cattle residing in northern portions of the us. an 8-year-old cow (maine anjou x angus cross-bred) in michigan presented with hemoglobinuria and a packed cell volume of 9. blood films stained with giemsa showed numerous intraerythrocytic parasites morphologically consistent with t. buffeli. the parasite was confirme ... | 2002 | 11900924 |
| the phylogenetic position of the theileria buffeli group in relation to other theileria species. | theileria parasites known as either t. buffeli, t. orientalis or t. sergenti share many characteristics and are referred to as the t. buffeli group. the 18s ribosomal rna and merozoite-piroplasm surface protein-encoding genes display a wider genetic variation within the t. buffeli group than between well defined species like t. annulata and t. parva. analysis of 18s rrna gene sequences from the database showed that similar groups of related theileria parasites occur in wild ruminants in japan an ... | 2002 | 12051603 |
| molecular phylogenetic study of theileria sp. (thung song) based on the thymidylate synthetase gene. | theileria type thung song is an indigenous hemoparasite of dairy cattle from the south of thailand. it has previously been classified using the analysis of a comparative set of small subunit ribosomal rna nucleotide sequences. however, the classification of this parasite is still questionable since the theileria type thung song was located as the intermediate parasite between pathogenic and benign groups. we use the thymidylate synthetase gene (ts) as an alternative for the rapid molecular phylo ... | 2002 | 12051604 |
| phylogenetic analysis of theileria sp. from sika deer, cervus nippon, in japan. | the 18s rrna genes of theileria species detected in sika deer, cervus nippon centralis in yamaguchi and cervus nippon yesoensis in hokkaido, were analyzed. the percent identities of the nucleotide sequences of theileria from cervus nippon centralis and cervus nippon yesoensis were more than 99%. the percent identities of the theileria sp. from sika deer and theileria sergenti, theileria buffeli and theileria cervi were 97, 96 and 95%, respectively. phylogenetic analysis of the gene sequences als ... | 2004 | 15063944 |
| molecular and phylogenetic characterization of theileria spp. parasites in autochthonous bovines (mirandesa breed) in portugal. | a survey was conducted during the months of april-june 2003 in the northeast portugal (bragança district) in order to characterize the hemoparasite population of an autochthonous mirandesa breed of bos taurus. the polymerase chain reaction (pcr) analysis of the bovine blood revealed that 3 out of 116 animals were infected with theileria and/or babesia parasites, while reverse line blot hybridisation (rlb) analysis showed that these animals were infected with theileria buffeli/orientalis. cloning ... | 2004 | 15265567 |
| molecular diagnosis of theileria and babesia species infecting cattle in northern spain using reverse line blot macroarrays. | piroplasmosis in cattle is caused by tick-borne haemoprotozoan parasites of the genera theileria and babesia. molecular detection techniques offer higher sensitivity and specificity than microscopy examination methods and serological tests. a reverse line blot (rlb) macroarray that included generic and species-specific probes for theileria annulata, theileria buffeli, babesia bovis, babesia bigemina, babesia divergens and babesia major was used to study the presence and identity of the piroplasm ... | 2006 | 16684356 |
| detection and quantification of anaplasma marginale dna in blood samples of cattle by real-time pcr. | a taqman-based real-time pcr assay was developed for the diagnosis of anaplasma marginale infection of cattle. the established assay was proven to be highly specific, since no cross-reactions were observed with other anaplasma species of ruminants, including the closely related anaplasma centrale, or other haemoparasites of ruminants (anaplasma bovis, anaplasma ovis, anaplasma phagocytophilum, babesia bovis, babesia bigemina, theileria annulata and theileria buffeli). the detection limit was equ ... | 2007 | 17466470 |
| sequence polymorphism in the ribosomal dna internal transcribed spacers differs among theileria species. | the genomic region spanning the two ribosomal rna internal transcribed spacers (its1 and its2) and the 5.8s rrna gene was cloned and sequenced from sixteen theileria isolates. each theileria species possessed its1 and its2 of unique size(s) and species specific nucleotide sequences. varying degrees of its1 and its2 intra- and inter-species sequence polymorphism were found among ruminant theileria species. the spacers were most polymorphic in the agent of tropical theileriosis, theileria annulata ... | 2007 | 17513053 |
| epidemiological studies on tick-borne diseases of cattle in central equatoria state, southern sudan. | a herd-based study was carried out in central equatoria state, southern sudan, to study epidemiological aspects of tick-borne diseases. six herds of cattle situated in three different locations were selected and investigated every 3 months during the year 2005. blood smears for giemsa staining and blood spots on filter paper for deoxyribonucleic acid extraction were collected from 600 apparently healthy indigenous cattle. a total of 69 (11.5%) samples showed the presence of piroplasms in giemsa- ... | 2007 | 17530477 |
| ovine theileriosis in china: a new look at an old story. | a fatal disease of sheep and goats in the northwestern part of china has in the past been reported to be due to theileria lestoquardi. however, some characteristics of the causative agent are not in accordance with attributes ascribed to this parasite. we therefore determined the nucleotide sequence of the 18 small subunit ribosomal rna (18s rrna) gene of t. lestoquardi and the parasite identified in china and compared it with that of other theileria and babesia species. in the inferred phylogen ... | 2007 | 17823827 |
| [use of multiplex pcr for the diagnosis of theileria annulata and theileria buffeli]. | the species causing theileriosis in cattle in turkey are theileria annulata and t. buffeli. while t. buffeli is low in pathogenicity or non-pathogenic , t. annulata is very pathogenic and causes tropical theileriosis with high morbidity and mortality in cattle. in this study, a multiplex pcr was used for a simultaneous diagnosis of these species. genes for the merozoite surface antigen (tams 1) and the major piroplasm surface protein (mpsp) were amplified with pcr for t. annulata and t. buffeli, ... | 2008 | 18351541 |
| hemoprotozoa of domestic animals in france: prevalence and molecular characterization. | very limited information is available on epizootiology of haematozoan infections in french domestic animals. in an attempt to address this issue, prevalence of piroplasmida was studied in carnivores and ruminants, whereas prevalence of hepatozoon spp. was only investigated in carnivores. in total, 383 animals were included in the survey (namely 116 cats, 108 dogs, 91 sheep and 68 cows). parasite diagnosis was carried out using molecular methods such as pcr and sequencing of the 18s rrna gene. in ... | 2009 | 19013719 |
| mediterranean theileriosis and other tick transmitted piroplasmoses in cattle in minorca (balearic islands, spain): the effect of tick control on prevalence levels analyzed by reverse line blot (rlb) macroarrays. | mediterranean theileriosis, caused by theileria annulata, is endemic in minorca (balearic islands, spain). the present study analyzes the prevalence of piroplasm infections in cattle in minorca using reverse line blot (rlb) macroarrays, as well as the effect of herd tick control on those prevalence levels. one hundred and nineteen animals from 12 herds were sampled in representative areas of the island. information was gathered regarding tick control for the animals (frequency and acaricide used ... | 2009 | 19086744 |
| a molecular survey of piroplasmida and hepatozoon isolated from domestic and wild animals in burgos (northern spain). | this study reports a molecular survey of hepatozoon species and of the order piroplasmida in the province of burgos, northern spain. the diagnostic techniques employed included pcr and the sequencing of the 18s rrna gene. eighty-nine blood samples from domestic animals plus 138 blood/coagulated blood samples from wild mammals were examined. theilerid protozoa were found at relatively high frequencies in bovines (14.6%) and horses (36%). theileria buffeli, t. sergenti and t. annulata were diagnos ... | 2009 | 19297099 |
| prevalence and diagnosis of babesia and theileria infections in horses in italy: a preliminary study. | babesia caballi and theileria equi are the causative agents of equine piroplasmosis. in this preliminary epidemiological study, 412 horses reared in central and northern italy were sampled and three diagnostic methods compared, namely, the microscopy, the indirect fluorescent antibody test (ifat) and a pcr. possible risk factors (such as area, season, breed, activity, sex, age, and grazing) associated with serological positivity were evaluated. a seroprevalence of 68.4% was found: 12.4% of the a ... | 2010 | 19394253 |
| haemolytic anaemia in cattle in nsw associated with theileria infections. | several outbreaks of anaemia, jaundice, abortion and mortality in cattle in new south wales were attributed to the intracellular parasite, theileria buffeli. disease occurred predominantly in periparturient animals that had been moved from inland to coastal areas. diagnosis was made via exclusion of other causes of haemolytic anaemia and observation of the parasite in blood smears. treatments included both registered and non-registered products. there is a possibility of a new strain of theileri ... | 2010 | 20148827 |
| theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around lake mburo national park in uganda. | wildlife, especially cape buffalo (syncerus caffer), are thought to act as a reservoir for many of the important tick-borne pathogens of cattle. in this study, we have determined the prevalence of the most significant tick-borne haemoparasites in wildlife (buffalo, impala, eland and bushbuck) as well as in cattle grazing inside and neighbouring lake mburo national park (lmnp) in uganda. a high percentage of buffalo were carriers of theileria parva, theileria mutans, theileria velifera, theileria ... | 2011 | 20827491 |
| detection of babesia and theileria species infection in cattle from portugal using a reverse line blotting method. | babesiosis and theileriosis are tick-borne diseases widespread in tropical and sub-tropical regions with high economic impact worldwide. in portugal there are at least 4 tick vectors known to be competent for the transmission of babesia and theileria sp. identified: rhipicephalus bursa, rhipicephalus (boophilus) annulatus, ixodes ricinus and haemaphysalis punctata. all these potential babesia and theileria tick vectors are widely distributed in portugal, although they are predominant in the sout ... | 2010 | 21036481 |
| an outbreak of haemolytic anaemia associated with infection of theileria orientalis in naive cattle. | an outbreak of haemolytic anaemia occurred when 87 cattle were introduced from a presumed non-infected herd from south otago to a herd in northland (n=580 cows), new zealand, where theileriosis is endemic. | 2011 | 21409734 |
| occurrence of theileria and babesia species in water buffalo (bubalus babalis, linnaeus, 1758) in the hubei province, south china. | the presence and prevalence of tick-borne haemoparasites in water buffalo from the hubei province, south china was investigated using the reverse line blot (rlb) hybridization assay and phylogenetic analysis of the parasite 18s rrna gene. theileria buffeli (19.1%) was the most frequently found species in all of the locations, followed by babesia orientalis (8.9%), babesia bovis (1.0%) and babesia bigemina (0.7%). only 12 (3.9%) of the samples had mixed infections. eleven samples with single infe ... | 2011 | 22154255 |
| Bovine theileriosis - An emerging problem in south-eastern Australia? | Bovine theileriosis is an arthropod-borne disease caused by one or more haemoprotozoan parasites of the genus Theileria. Traditionally, Theileria infection in cattle in Australia was largely asymptomatic and recognized to be associated with Theileria buffeli, now assigned to the Theileria orientalis-group. There have been some recent outbreaks of theileriosis in dairy and beef cattle, mainly in subtropical climatic zone (New South Wales) of Australia. Here, we provide the first published evidenc ... | 2011 | 21906697 |
| a highly sensitive dna bead-based suspension array for the detection and species identification of bovine piroplasms. | piroplasms are among the most harmful tick-borne pathogens for livestock and sensitive and specific diagnostic methods for rapid detection and identification of the different species are needed for effective control. reverse line blot has been the molecular technique of choice but it is laborious, time-consuming and highly susceptible to subjective variation in the interpretation of the hybridisation signal. here, an oligonucleotide multiplex suspension microarray (luminex® microsphere system) w ... | 2011 | 22233830 |
| retrospective study of hemoparasites in cattle in southern italy by reverse line blot hybridization. | tick-borne diseases are widespread in tropical and temperate regions and are responsible for important economic losses in those areas. in order to assess the presence and prevalence of various pathogens in southern italy, we retrospectively analyzed cattle blood samples collected for a previous study in 2000 using reverse line blot (rlb) hybridization. the study had been carried out in three regions of southern italy on 1,500 randomly selected and apparently healthy adult cattle. rlb showed that ... | 2014 | 24614604 |
| re-emergence of bovine piroplasmosis in hungary: has the etiological role of babesia divergens been taken over by b. major and theileria buffeli? | the prevalence of bovine babesiosis caused by babesia divergens has been declining during the past decades in northeastern hungary, and no cases have been observed since 2008. infections of cattle with b. major and theileria buffeli were hitherto reported in southern and western europe. in other parts of the globe, there is evidence of emergence and a growing clinical importance of t. buffeli and closely related genotypes of the t. orientalis complex. | 2014 | 25212410 |
| molecular assays reveal the presence of theileria spp. and babesia spp. in asian water buffaloes (bubalus bubalis, linnaeus, 1758) in the amazon region of brazil. | approximately 50% of buffalo herds in brazil are located in pará state in northern brazil. there are several properties where cattle and buffalo live and graze together, and thus, buffalo pathogens may threaten the health of cattle and vice versa. therefore, knowledge of infectious agents of buffalo is essential for maintaining healthy livestock. clinical disease caused by theileria and babesia parasites in the asian water buffalo is not common, although these animals may act as reservoir hosts, ... | 2016 | 27344507 |
| molecular and parasitological survey of bovine piroplasms in the black sea region, including the first report of babesiosis associated with babesia divergens in turkey. | clinical cases of babesiosis were evaluated, and the frequency of bovine babesia and theileria parasites was determined in cattle. blood samples and thin blood smears were collected from 23 cattle exhibiting clinical signs of babesiosis. in addition, tick and blood samples were collected from 100 apparently healthy cattle cograzing from the same area. egg masses obtained from fully engorged female ticks were included. dna isolated from blood and tick samples was screened for babesia and theileri ... | 2015 | 26336265 |
| monitoring piroplasms infection in three cattle farms in minorca (balearic islands, spain) with previous history of clinical piroplamosis. | bovine piroplasmosis are tick-borne protozoan diseases caused by parasites of the genera theileria and babesia. three friesian cattle farms (f1-f3) with previous history of clinical piroplamosis were selected in minorca (balearic islands, spain). blood samples were collected from 8 to 11 animals every two months throughout a year and, a newly developed multiplex dna bead-based suspension array based on the luminex(®) xmap technology was used to monitor for the presence of piroplasms. the assay i ... | 2012 | 22884914 |
| prevalence of bovine theileriosis in north central region of algeria by real-time polymerase chain reaction with a note on its distribution. | to determine the presence and distribution of bovine theileriosis in the north central region of algeria, 358 dna samples and 359 blood smears were analyzed from nine provinces. theileria dna extracted from cattle blood was amplified by fluorescence resonance energy transfer polymerase chain reaction (fret-pcr). blood smears were examined for theileria piroplasms by microscopical examination (me) of giemsa-stained slides. while microscopical identification revealed only 42 animals being infected ... | 2015 | 25827097 |
| genetic diversity and phylogenetic analysis of tams1 of theileria annulata isolates from three continents between 2000 and 2012. | theileria annulata, which is part of the theileria sergenti/theileria buffeli/theileria orientalis group, preferentially infects cattle and results in high mortality and morbidity in the mediterranean, middle east, and central asia. the polypeptide tams1 is an immunodominant major merozoite piroplasm surface antigen of t. annulata that could be used as a marker for epidemiological studies and phylogenetic analysis. in the present study, a total of 155 tams1 sequences were investigated for geneti ... | 2014 | 26155166 |
| [survey of theileria annulata and theileria buffeli/orientalis complex in cattle in the kırşehir region using multiplex-pcr]. | to investigate the presence of t. annulata and t. buffeli/orientalis complex in the blood of 172 cattle selected from 9 different regions of kırşehir. | 2012 | 22450914 |
| genetic variability, phylogenetic evaluation and first global report of theileria luwenshuni, t. buffeli, and t. ovis in sheepdogs in iran. | theileriosis, caused by tick-borne hematozoan organisms, is a protozoan disease of domestic and wild mammals. the most phylo-molecular explorations of genus theileria have been globally focused on the livestock and here are few investigations about canine theileriosis. twenty three and 52 blood samples were collected from sheepdogs and their owners, respectively, at different geographical foci of mazandaran province (east, central, and west regions), northern iran. to primary identify, the taken ... | 2016 | 26997339 |
| phylogeny of theileria buffeli genotypes identified in the south african buffalo (syncerus caffer) population. | theileria buffeli/orientalis is a group of benign and mildly pathogenic species of cattle and buffalo in various parts of the world. in a previous study, we identified t. buffeli in blood samples originating from the african buffalo (syncerus caffer) in the hluhluwe-imfolozi game park (hip) and the addo elephant game park (aegp) in south africa. the aim of this study was to characterise the 18s rrna gene and complete internal transcribed spacer (its1-5.8s-its2) region of t. buffeli samples, and ... | 2014 | 25002308 |
| evaluation of a real-time pcr test for the detection and discrimination of theileria species in the african buffalo (syncerus caffer). | a quantitative real-time pcr (qpcr) assay based on the cox iii gene was evaluated for the simultaneous detection and discrimination of theileria species in buffalo and cattle blood samples from south africa and mozambique using melting curve analysis. the results obtained were compared to those of the reverse line blot (rlb) hybridization assay for the simultaneous detection and differentiation of theileria spp. in mixed infections, and to the 18s rrna qpcr assay results for the specific detecti ... | 2013 | 24146782 |
| molecular detection of piroplasms in ixodid ticks infesting cattle and sheep in western oromia, ethiopia. | in ethiopia, ticks and tick-borne diseases are widely distributed and contribute to important economic losses. several studies investigated the prevalence and species composition of ticks infesting ruminants; however, data on tick-borne pathogens are still scarce. during the study period from october 2010 to april 2011, a total of 1,246 adult ticks and 264 nymphs were collected from 267 cattle and 45 sheep in bako district, western oromia, ethiopia. the study showed infestation of 228/267 (85.4 ... | 2014 | 23846769 |
| mutation scanning-based analysis of theileria orientalis populations in cattle following an outbreak. | bovine theileriosis is a tick-borne disease caused by one or more hemoprotozoan parasites of the genus theileria. in the past, theileria infection in cattle in australia was largely asymptomatic and recognized to be associated with theileria buffeli. however, outbreaks of theileriosis have occurred in beef and dairy cattle in subtropical climatic regions (new south wales) of australia. there is also one published report of a recent theileriosis outbreak in a beef farm near seymour in the southea ... | 2012 | 22806470 |
| babesia, theileria, and hepatozoon species in ticks infesting animal hosts in romania. | babesia spp., theileria spp., and hepatozoon spp. are tick-transmitted apicomplexan parasites that cause several important diseases in animals. to increase current knowledge about the diversity of tick-transmitted pathogens in romania, we investigated the occurrence of babesia spp., theileria spp., and hepatozoon spp. in a wide range of tick species infesting animal hosts. we collected 852 ticks from 10 different animal species from 20 counties in romania. the assessment was based on detection o ... | 2017 | 28676905 |