| bovine saccocystosis: patholgic features of naturally occurring infection with sarcocystis cruzi. | naturally occurring bovine sarcocystosis caused death and illness in eight dairy heifers. clinical disease was characterized by cachexia, peripheral lymph node enlargement, and anemia. increased amounts of serum enzymes and anti-sarcocystis antibody titers were present in affected animal. macroscopic findings in two heifers at necropsy included emaciation and serous atrophy. necropsied heifers (no. 1 and 2) contained different developmental stages of sarcocystis cruzi; each stage was characteriz ... | 1979 | 112889 |
| pathophysiological changes in urine and blood from calves experimentally infected with sarcocystit cruzi. | two 8-week experiments were conducted to determine the relationships between nutritional stress and pathophysiological changes in male holstein calves infected with sarcocystis cruzi. calves were infected by oral inoculation with 200 000 s. cruzi sporocysts. in the first experiment weight gain reduction was greatest in inoculated calves during weeks 4 and 5 after inoculation. feed intake was reduced during the 5th week. erythrocyte count was reduced during week 5 and haemoglobin was reduced duri ... | 1979 | 120522 |
| fine structure of sarcocystis cruzi schizonts. | | 1977 | 410921 |
| production of sarcocystis cruzi sporocysts by dogs fed experimentally infected and naturally infected beef. | twenty-one coccidia-free dogs were fed either experimentally infected or naturally infected beef in 4 experiments. the pretreatment period ranged from days 9 to 33; 7 dogs shed their first sporocysts on day 12. the full length of the patent period was not determined because most dogs were still shedding sporocysts when experiments ended on days 40 and 60. although the patent period was long, sporocysts were not shed continuously; the total number of days sporocysts were actually shed ranged from ... | 1977 | 412939 |
| the prevalence of sarcocystis spp in dogs and red foxes. | protozoan parasites of the genus sarcocystis have been recognised for many years as intramuscular cysts of numerous vertebrates. it is only comparatively recently that the two-host nature of the life cycle has been recognised and that the intramuscular cysts are a stage in the developmental cycle of coccidian parasites of flesh eating mammals (fayer 1974, fayer and johnson 1973, 1974, rommel and others 1972, dubey 1976). carnivores ingest the intramuscular cysts from herbivores and presumably fr ... | 1978 | 416553 |
| fine structure of immature cysts of sarcocystis cruzi. | sarcocystis cruzi forms cysts in striated muscle of the bovine host following schizogony. the fine structure of the immature cyst within muscle fibers of the ventricular myocardium was studied in relation to its development and to the multiplication of parasites within it. the young cyst is enclosed by a cyst wall containing numerous small protuberances. metrocytes within the cyst are irregular in shape and are separated from each other and the cyst wall by a thin layer of ground substance. the ... | 1978 | 417164 |
| a review of sarcocystis of domestic animals and of other coccidia of cats and dogs. | the nomenclature, life cycles, and pathogenicity of sarcocystis of domestic animals are reviewed. sarcocystis had a 2-host life cycle, with carnivores as definitive hosts and herbivores as intermediate hosts. the following species are found in domestic animals (with the definitive hosts given in parentheses): 3 species in the ox: s cruzi (dog, wolf, coyote, raccoon, fox), s hirsuta (cat), s hominis (man, monkey); 2 species in the sheep: s ovicanis (dog), s tenella (cat); 3 species in the pig: s ... | 1976 | 824260 |
| equine protozoal myelitis in panamanian horses and isolation of sarcocystis neurona. | schizonts of sarcocystis neurona were identified microscopically in hematoxylin-eosin-stained spinal cord sections from 2 native panamanian horses that exhibited clinical signs of equine protozoal myelitis (epm). spinal cord homogenate from a third panamanian horse with epm was inoculated onto monolayers of cultured bovine monocytes (m617). intracytoplasmic schizonts containing merozoites arranged in rosette forms surrounding a central residual body first were observed 13 wk postinoculation. par ... | 1992 | 1403437 |
| ultrastructural and transmission evidence of sarcocystis cruzi associated with eosinophilic myositis in cattle. | skeletal muscle, diaphragm, tongue, esophagus and heart of beef carcasses that were condemned for eosinophilic myositis and those that were unaffected were collected at an abattoir in colorado and studied to determine the involvement of sarcocystis spp. all affected carcasses contained similar granulomatous lesions with adjacent infiltrations of leukocytes. intact or fragments of sarcocysts were found within 32 of 363 granulomas, and whole sarcocysts were present in nearby unaffected muscle cell ... | 1992 | 1586892 |
| development of genomic probes to sarcocystis cruzi (apicomplexa). | a genomic library of sarcocystis cruzi sporozoite dna was constructed in bacteriophage lambda gt10. recombinant phages containing insert dna were selected by growth on escherichia coli strain c600 hfla150. of 14 clones examined, 11 contained dna inserts ranging in size from approximately 1.45 kilobase (kb) to 6.18 kb. insert dna from four of these clones specifically hybridized to 32p-labelled s. cruzi merozoite dna. one of these insert dna, clone sl41, was selected and labelled with 32p. this p ... | 1991 | 1837193 |
| sarcocystis neurona n. sp. (protozoa: apicomplexa), the etiologic agent of equine protozoal myeloencephalitis. | sarcocystis neuronan n. sp. is proposed for the apicomplexan taxon associated with myeloencephalitis in horses. only asexual stages of this parasite presently are known, and they are found within neuronal cells and leukocytes of the brain and spinal cord. the parasite is located in the host cell cytoplasm, does not have a parasitophorous vacuole, and divides by endopolygeny. schizonts are 5-35 microns x 5-20 microns and contain 4-40 merozoites arranged in a rosette around a prominent residual bo ... | 1991 | 1901359 |
| sarcocystis canis n. sp. (apicomplexa: sarcocystidae), the etiologic agent of generalized coccidiosis in dogs. | sarcocystis canis n. sp. is proposed for the protozoon associated with encephalitis, hepatitis, and generalized coccidiosis in dogs. only asexual stages are known in macrophages, neurons, dermal, and other cells of the body. the parasite is located free in the host cell cytoplasm without a parasitophorous vacuole; schizonts divide by endopolygeny. schizonts are 5-25 x 4-20 microns and contain 6-40 merozoites. merozoites are approximately 5-7 microns x 1 micron and do not contain rhoptries. the p ... | 1991 | 1907653 |
| plasma and tissue concentrations and molecular forms of somatostatin in calves infected with sarcocystis cruzi. | the effects of parasitic infection on plasma and tissue content of immunoreactive somatostatin (srif) were studied in 4-mo old male calves inoculated with the protozoan sarcocystis cruzi. because feed intake significantly decreased (70%) in infected calves around day 28 postinfection (pi), concomitant with the asexual replication of s. cruzi and outward expression of clinical signs, the relative contributions of infection and associated reduction in nutrition on plasma srif were evaluated. treat ... | 1990 | 1979766 |
| continuous in vitro cultivation of sarcocystis cruzi. | sporozoites of sarcocystis cruzi were inoculated onto monolayer cultures of bovine pulmonary artery endothelial (cpa) cells. sporozoites entered the cells, formed large and small multinucleate schizonts, and produced large numbers of merozoites. continuous cultivation from the original sporozoite inoculum has been maintained for more than 1,320 days by subinoculating merozoites onto new cultures of cpa cells. during this time, the capacity to produce both types of schizonts was preserved, and sc ... | 1990 | 2108236 |
| immunodominant proteins of sarcocystis cruzi bradyzoites isolated from cattle affected or nonaffected with eosinophilic myositis. | sarcocystis cruzi sarcocysts were isolated from eosinophilic myositis (em)-affected and nonaffected bovine hearts. isolates were ruptured and used to prepare a bradyzoite antigen extract from each heart. the nonaffected heart from one newborn calf contained no apparent sarcocysts when examined histologically and was used to prepare sarcocystis-negative control antigen. blood samples were taken from the heart approximately 20 minutes after slaughter. serum was obtained and evaluated, using a radi ... | 1990 | 2117869 |
| immunofluorescent localization of sarcocystis cruzi antigens, igg and ige, in lesions of eosinophilic myositis in cattle. | | 1990 | 2128816 |
| types of myofibers parasitized in experimentally induced infections with sarcocystis cruzi and sarcocystis capracanis. | eighteen calves were orally inoculated with either 200,000 or 225,000 sporocysts of sarcocystis cruzi. eight goats were orally inoculated with 20,000 sporocysts of s capracanis. calves and goats were euthanatized at various times after inoculation, and portions of their right and left biceps femoris, right and left longissimus dorsi, myocardium, and tongue were frozen at -150 c in precooled isopentane and stored at -70 c. frozen sections of these muscles were stained with hematoxylin and eosin, ... | 1986 | 2421618 |
| influence of parasitism on plasma concentrations of growth hormone, somatomedin-c and somatomedin-binding proteins in calves. | a parasitic disease model (sarcocystosis) was used to study the effects of infection and associated plane of nutrition on gh and somatomedin-c (sm-c) patterns in plasma, and sm-c binding protein patterns in plasma from 4-month-old male holstein calves. calves, matched by age and rate of growth before the experiment, were divided into three treatment groups (n = 7). in the first (control), animals were uninfected and food was available ad libitum; in the second, animals were infected with sarcocy ... | 1988 | 2450946 |
| identification of antigens of sarcocystis cruzi sporozoites, merozoites, and bradyzoites with monoclonal antibodies. | sporozoites and culture-derived merozoites of sarcocystis cruzi were used to elicit monoclonal antibodies (mab's) in mice. some of these antibodies reacted with the surface of live sporozoites and merozoites as determined by immunofluorescence. an array of similar antigens was identified in western blots of sporozoites by both anti-merozoite mab's and an anti-sporozoite mab. at least 1 antigen in blots of bradyzoites was identified by anti-merozoite mab's and a cluster of antigens was identified ... | 1988 | 2458442 |
| type-i hypersensitivity as a component of eosinophilic myositis (muscular sarcocystosis) in cattle. | eight bovine hearts with lesions of eosinophilic myositis (em) and 2 bovine hearts without em lesions were collected at slaughter. blood samples from these 10 hearts, and the heart of a newborn calf also were collected. histologically, sarcocystis cruzi was identified in the 8 hearts with em lesions and the 2 hearts without em lesions, but not in the heart of the newborn calf. serum was harvested from the 10 blood samples and was used in homologous, modified, passive cutaneous anaphylaxis tests. ... | 1989 | 2496630 |
| elevated igg antibody to sarcocystis cruzi associated with eosinophilic myositis in cattle. | blood sera, skeletal muscle, and cardiac muscle from 24 bovine carcasses condemned for eosinophilic myositis by us department of agriculture meat inspection veterinarians were compared with similar specimens from 35 random carcasses passed for human consumption. fluorescence values determined by using a fluorometric immunoassay system were used to measure the specific antibodies to sarcocystis cruzi. the values were significantly elevated in carcasses condemned for eosinophilic myositis as compa ... | 1989 | 2518705 |
| specificity and cross-reactivity of hybridoma antibodies generated against eimeria bovis sporozoites. | spleens from mice immunized with eimeria bovis sporozoites were removed and the cells fused with mouse myeloma cells to produce hybridoma cell lines (hcls). the resulting hcls were examined for antibody (hab) production against e. bovis sporozoites using an indirect immunofluorescent antibody test on air-dried sporozoites. four fusions resulted in the production of 19 hcls that produced habs to e. bovis sporozoites. these 19 hcls were further tested for reactivity with cell culture-grown merozoi ... | 1989 | 2672546 |
| immunohistochemical diagnosis of neospora caninum in tissue sections. | an avidin-biotin-peroxidase complex immunoperoxidase staining method was developed to detect neospora caninum in formalin-fixed, paraffin-embedded tissue sections. specific antiserum to n caninum was made in rabbits and used to probe tissues from dogs naturally and experimentally infected with n caninum. the test detected tachyzoites and bradyzoites of n caninum. a reaction was not observed to toxoplasma gondii, hammondia hammondi, sarcocystis cruzi, s capricanis, s tenella, besnoitia jellisoni, ... | 1989 | 2694869 |
| hematologic values in calves infected with sarcocystis cruzi. | calves were inoculated with 2 x 10(5) sarcocystis cruzi sporocysts. red cell mass decreased dramatically between days 21 and 35 post-infection and plasma volume increased concurrently, so that blood volume did not change significantly. mild reticulocytosis and increased pyrimidine 5' nucleotidase activity in erythrocytes occurred between days 35 and 42. antiglobulin tests with anti-bovine igg, igm and c3 were negative, with the exception of a positive test for c3 in 1 of 6 infected calves. | 1986 | 3010540 |
| an outbreak of sarcocystosis in dairy cattle. | sixteen of 32 friesian calves, 8 to 10 weeks old, died over 4 weeks. the calves were housed in pens previously used by dogs. clinical signs included anorexia, pale mucous membranes, rapid weight loss, coughing and palpably enlarged superficial lymph nodes. at necropsy, calves were emaciated and had generalised enlargement of lymph nodes, pale mottling of skeletal muscles, excess peritoneal, thoracic and pericardial fluid and subpleural and subepicardial haemorrhages. histologically there was a l ... | 1986 | 3082319 |
| in vitro cultivation of meronts of sarcocystis cruzi. | several established cell lines were tested for their ability to support in vitro development of meronts of sarcocystis cruzi. sporozoites penetrated bovine monocytes (bm), bovine pulmonary artery endothelial cells (cpa), madin-darby bovine kidney cells and mouse macrophages, but developed to meronts in bm and cpa only. sporozoites developed to large meronts that contained approximately 180-350 merozoites, whereas merozoites formed small meronts with 50-100 merozoites. mature large meronts were p ... | 1986 | 3100756 |
| in vitro excystation of sarcocystis capracanis, sarcocystis cruzi and sarcocystis tenella (apicomplexa). | improved rates of in vitro excystation of sporozoites from sporocysts of sarcocystis capracanis, sarcocystis cruzi, and sarcocystis tenella were obtained by pretreating sporocysts with an aqueous sodium hypochlorite (naocl) solution followed by incubation in excysting fluid (ef). after pretreatment with naocl, sporocysts were washed 4 times in hanks' balanced salt solution and then incubated in various ef (ph 7.4) at 38.5 c in 5% co2-95% air. maximum rates of excystation (free sporozoites/(sporo ... | 1986 | 3102713 |
| the prevalence and identity of sarcocystis in beef cattle in new zealand. | muscle tissue from the oesophagus and diaphragm of 500 beef cattle slaughtered in new zealand was examined for sarcocystis infection by microscopic examination of cysts isolated from muscle samples. all cattle were infected with sarcocystis; based on light microscopy of cysts, 98% had thin-walled sarcocystis cruzi cysts and 79.8% had thick-walled (sarcocystis hirsuta/sarcocystis hominis) cysts. cysts were also collected for electron microscopy and transmission experiments. thick-walled cysts cou ... | 1987 | 3113040 |
| serological responses in sarcocystis cruzi infected calves challenged with toxoplasma gondii. | three four-week-old calves were used in a study on serological responses in combined sarcocystis cruzi and toxoplasma gondii infections. one calf (c1) was inoculated orally with 100,000 s cruzi sporocysts, another (c2) with 40,000 sporocysts, while the third (c3) acted as uninfected control. one hundred and five days later, all three calves were inoculated orally with 100,000 t gondii oocysts. the antibody responses to sarcocystis and toxoplasma during 210 days were recorded, using different ser ... | 1987 | 3114848 |
| association of eosinophilic myositis with an unusual species of sarcocystis in a beef cow. | the carcass of a mature cow had numerous, disseminated lesions typical of eosinophilic myositis. to elucidate the nature and possible cause of the lesions, histological sections were examined by light microscopy and selected areas were removed and processed for electron microscopy. the lesions were granulomatous in nature. each granuloma contained at its centre an intact or ruptured sarcocyst associated with degenerate muscle fibers. surrounding this was a layer of epithelioid cells and an inten ... | 1987 | 3115553 |
| lysates of sarcocystis cruzi bradyzoites stimulate raw 264.7 macrophages to produce tumor necrosis factor (cachectin). | lysate of sarcocystis cruzi bradyzoites induced raw 264.7 macrophages to release a factor that killed l 929 cells. rabbit antibody to murine tumor necrosis factor (tnf) inhibited this killing effect, indicating that the factor causing cell death was similar to tnf (also called cachectin). based on the present findings we hypothesize that tnf mediates, at least in part, the disease associated with this parasitic infection. | 1988 | 3135378 |
| [development of sarcocystosis in experimentally infected calves]. | in seven calves we studied experimental invasions by sporocysts of the sarcocystis cruzi (s. bovicanis) species, isolated from faeces of dingo dogs. out of clinical changes, an increase in body temperature to 39.6 to 40.5 degrees c is characteristic in the fourth to the eighth week of disease, relaxed attitude of animals, progressive thinning down, anaemia of mucous membranes, diarrhoea and total dehydration. the post-mortem examination completes this observation with generalized hyperplasia of ... | 1985 | 3918381 |
| plasma alpha-fetoprotein concentrations in pregnant cows exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus. | bovine alpha-fetoprotein (afp) was determined in maternal plasma, using radioimmunoassay in an attempt to detect and monitor fetal distress in pregnant cows. plasma from pregnant cows in the 4th to 5th month of the gestation which had been exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus was used. plasma afp concentrations were determined at intervals from before the cows were exposed until they had aborted or calved. the plasma afp concentration of the exposed pregnan ... | 1981 | 6176152 |
| cytochemical studies on nuclear dna of four eucoccidian parasites, isospora (toxoplasma) gondii, eimeria tenella, sarcocystis cruzi and plasmodium berghei. | feulgen-pararosaniline (so2) staining was performed on stages in the life-cycle of isospora (toxoplasma) gondii, eimeria tenella, sarcocystis cruzi and plasmodium berghei. the fluorescence emission of the stained dna in nuclei of these stages was examined and compared with absorption microscopy measurements at 560 nm (green light) of the same specimens. accurate identification of single cells, and especially discrimination between young schizonts and young gamonts was difficult after feulgen sta ... | 1984 | 6200820 |
| determination of nuclear dna of five eucoccidian parasites, isospora (toxoplasma) gondii, sarcocystis cruzi, eimeria tenella, e. acervulina and plasmodium berghei, with special reference to gamontogenesis and meiosis in i. (t.) gondii. | dna contents of individual stages of isospora (toxoplasma) gondii and other eucoccida were measured after feulgen-pararosaniline (so2) staining either by direct microfluorometry or by scanning of microphotographic negatives. frequency distributions were analysed using a computer program based on a mathematical model describing cell division. all stages of i. (t.) gondii, except fertilized macrogametes (2c), contained a haploid amount of dna (1c), indicating that meiosis in i. (t.) gondii occurs ... | 1984 | 6204268 |
| experimental infections of sarcocystis cruzi, sarcocystis tenella, sarcocystis capracanis and toxoplasma gondii in red foxes (vulpes vulpes). | four littermate 6-wk-old red foxes (nos. 1-4) were fed toxoplasma gondii, sarcocystis cruzi, s. tenella and s. capracanis. one littermate fox (no. 5) served as the control. two foxes (nos. 1, 2) were fed tissue cysts of t. gondii and two foxes (nos. 3, 4) were fed oocysts of t. gondii. twenty-one to 42 days later, the same five foxes were used to test the infectivity of meat of goat, sheep, and ox experimentally inoculated with sarcocystis. fox 2 was fed goat meat and shed s. capracanis-like spo ... | 1983 | 6417347 |
| hematologic and coagulation abnormalities in acute bovine sarcocystosis. | calves inoculated with sarcocystis cruzi sporocysts developed severe anemia 4 weeks later. the anemic crisis was paralleled by a hyperbilirubinemia, with up to 88% of the increased total bilirubin attributed to indirect reacting bilirubin. the anemia was characterized as normocytic and normochromic. in a few instances, according to coombs's tests (antiglobulin test) and erythrocyte eluates from infected calves, immunoglobulin g was associated with the rbc membrane. in the histopathologic examina ... | 1984 | 6422807 |
| humoral and cellular immune responses in cattle and sheep inoculated with sarcocystis. | cattle inoculated with sarcocystis bovicanis (= sarcocystis cruzi) and sheep inoculated with sarcocystis ovicanis were monitored for the appearance of sarcocystis-specific antibodies and lymphocytes in the peripheral circulation. anti-sarcocystis antibody was identified by enzyme-linked immunosorbent assay, whereas antigen-reactive lymphocytes were discerned by an in vitro lymphocyte blastogenic assay. the antigens used were the soluble fraction recovered from disrupted bradyzoites of mature sar ... | 1984 | 6433756 |
| [acute sporidiosis in cattle. a case of clinically natural meningoencephalitis caused by sarcocystis cruzi in a bull]. | | 1984 | 6435283 |
| protective immunity against clinical sarcocystosis in cattle. | five experiments involving 50 calves (bos taurus) were conducted to determine if oral infection with sporocysts of the pathogen sarcocystis cruzi or the non-pathogen sarcocystis hirsuta would stimulate development of a protective immunity against illness or death from challenge infection with large numbers of s. cruzi sporocysts. four experiments involved holstein calves housed in individual pens in isolation buildings; one experiment involved hereford calves on an open range. calves infected wi ... | 1984 | 6437053 |
| plasma progesterone concentration in the bovine before abortion or parturition in pregnant animals exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus. | pregnant cows in the 4th and 5th month of the gestation were exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus. plasma progesterone concentrations were determined at intervals from before the cows were exposed until they had aborted or calved. the plasma progesterone concentration of the exposed pregnant cattle remained at 3.95 +/- 2.0 ng/ml until 24 to 48 hours before abortion or parturition, when it decreased to below 1.00 ng/ml. this pattern was similar for cattle ex ... | 1980 | 6782916 |
| development and ultrastructure of first-generation meronts of sarcocystis cruzi in calves fed sporocysts from coyote feces. | the development of sarcocystis cruzi hasselmann (syn. s. fusiformis railliet) meronts was studied in seven 7- to 10-day-old calves filled 4, 7, 11, 15, 22, 25 and 28 days postinoculation (dpi) with 5 x 10(7) sporocysts from feces of coyotes. no meronts were found 4 and 7 dpi. young and intermediate meronts with 1-16 nuclei were found in endothelial cells of arteries in mesenteric lymph nodes, but not in kidneys 11 dpi. mature meronts were noted in endothelial cells of arteries, arterioles, or ca ... | 1980 | 6783745 |
| prepartum changes of plasma concentrations of prostaglandin f and 13,14-dihydro-15-ketoprostaglandin metabolites in pregnant animals exposed to sarcocystis cruzi or campylobacter fetus. | pregnant cows at 4- to 5-months' of gestation were exposed to sarcocystis cruzi or campylobacter fetus. plasma prostaglandin f (pgf) and 13,14-dihydro-15-ketoprostaglandin metabolite (pgm) concentrations were determined at intervals from before exposure until abortion or parturition. the plasma pgf concentration of pregnant infected cattle remained at 0.02 +/- 0.04 ng/ml until 24 to 48 hours before abortion or parturition when it increased 5-fold to 0.11 +/- 0.12 ng/ml. the plasma pgm concentrat ... | 1981 | 6784618 |
| sarcocystosis in cattle and sheep at prague abattoir. | a total of 288 animal oesophagi, including 154 from cattle and 134 from sheep of various age, were examined for the presence of sarcosporidians at prague abattoir. the material from cattle was positive in 129 cases (84%) and all the three species of sarcosporidians hitherto described from cattle were identified: sarcocystis cruzi, s. hirsuta and s. hominis. among the sheep, 110 animals (82%) were infected. the macroscopic species s. tenella was found in 23 cases. the remaining positive findings ... | 1981 | 6786962 |
| [finding of sarcocystis cruzi in a dog of the city of bologna]. | | 1980 | 6796929 |
| early developmental stages of sarcocystis cruzi in calf fed sporocysts from coyote feces. | the development of sarcocystis cruzi was studied in an 11-day-old calf killed seven days postinoculation with 5 x 10(8) sporocysts from feces of coyotes. uninucleate zoites were found in arteries of mesenteric lymph nodes but not in other organs. zoites measured 4.9 x 3.0 (3.5-7.0 x 2.1-3.5) micrometers. of the 36 zoites studied, 31 were in endothelial cells, four were in macrophages in the lumen of arteries, and one was free in the lumen of an artery. infected endothelial cells were two to thre ... | 1981 | 6798207 |
| ultrastructure of in vivo lysis of sarcocystis cruzi merozoites. | | 1981 | 6799635 |
| sporocysts of sarcocystis cruzi in mesenteric lymph nodes of dogs. | | 1981 | 6804877 |
| quantitative parasitemia in calves fed sarcocystis cruzi sporocysts from coyotes. | for the quantitation of parasitemia, seven 7- to 10-day-old calves were orally inoculated with 2 virulent isolates of sarcocystis cruzi from coyotes. three calves were each given 2.5 million sporocysts of the 1st isolate; these calves died 31, 35, and 33 days later. the other 4 calves were given 2, 1, 0.76, and 0.38 million sporocysts of the 2nd isolate; these calves died or were euthanatized at 34, 44, 43, and 55 days after inoculation. buffy coat smears from 7 ml of jugular blood of each calf ... | 1982 | 6808868 |
| sarcocystosis in neonatal bison fed sarcocystis cruzi sporocysts derived from cattle. | a 6-day-old female (bison bison) was inoculated with 10 million sporocysts of the b1 isolate of sarcocystis cruzi originally obtained by feeding heart of a naturally infected cow (bos taurus) to a laboratory-raised coyote. the bison became febrile, lethargic, and anorectic at about 25 days after inoculation of the sporocysts, and was euthanatized 3 days later. there were widespread hemorrhages, hepatitis, myocarditis, nephritis, and enteritis; intravascular meronts were found in the adrenal cort ... | 1982 | 6816777 |
| development of ox-coyote cycle of sarcocystis cruzi. | | 1982 | 6816926 |
| effectiveness of methods used for the detection of sarcosporidiosis in farm animals. | trypsinization and stereoscopy were found to be the most suitable methods for the detection of sarcosporidiosis in farm animals. sarcocystis cruzi, s. hirsuta and s. hominis were detected in 87% of 200 cattle and s. ovicanis and s. tenella in 92% of 100 sheep examined. the examinations of 200 pigs were negative. | 1982 | 6819215 |
| sarcocystosis in newborn calves fed sarcocystis cruzi sporocysts from coyotes. | the development of sarcocystis cruzi was compared in 3- to 14-day-old calves (n = 38) inoculated with 55,000 to 500,000,000 sporocysts from intestinal scrapings of coyotes, and euthanatized and necropsied on postinoculation days (pid) 4 to 153. from pid 15 to 19, the calves remained clinically normal, except for fever (greater than or equal to 40 c), during the development of 1st-generation meronts, and lesions in calves euthanatized at this time were confined mainly to mesenteric lymph nodes an ... | 1982 | 6819793 |
| specific amplification of sarcocystis cruzi dna using a randomly primed polymerase chain reaction assay. | the polymerase chain reaction (pcr) method to randomly amplify polymorphic dna (rapd) was used to differentiate between sarcocystis cruzi dna and bovine dna. this assay was also exploited to identify a s. cruzi dna fragment which may be useful as a probe. five primers ranging in length from 16 to 20 nucleotides were analyzed for their ability to direct the amplification of either bovine or parasite dna fragments. two primers, tga and tgd, preferentially amplified bovine dna in a mixture of s. cr ... | 1994 | 7725622 |
| the wisent (bison bonasus, bovidae) as an intermediate host of three sarcocystis species (apicomplexa: sarcocystidae) of cattle. | sarocysts were found in muscle tissue of a wisent (bison bonasus) which was born and kept in germany. light microscopic and tem examination revealed all the three named species known from cattle: sarcocystis cruzi ("thin-walled", with longer hair-like villar protrusions of the primary cyst wall); s. hirsuta ("thick-walled", with tongue-like protrusions of the cyst wall arising with very short and narrow stalklets from the surface of the cyst and containing rows of electron-dense granules in the ... | 1994 | 7927061 |
| encephalomyelitis due to a sarcocystis neurona-like protozoan in a rhesus monkey (macaca mulatta) infected with simian immunodeficiency virus. | a captive-born rhesus monkey (macaca mulatta) experimentally infected with simian immunodeficiency virus developed neurologic abnormalities approximately seven months postinoculation. a chronic necrotizing encephalomyelitis with intralesional protozoal schizonts was diagnosed histologically. the protozoa was identified as sarcocystis neurona based on its morphologic characteristics by light and electron microscopic examination, the developmental stages of the schizonts, and positive staining wit ... | 1994 | 7943553 |
| survey of sarcocystis infection in cattle in east hokkaido, japan. | the prevalence of sarcocystis infection was examined in older cows and imported cattle slaughtered in east hokkaido. samples of myocardial tissues were examined for sarcocystis microscopically. sarcocystis cysts were detected in 15.7% of 83 older cows, 48.4% of 91 imported cattle which were kept in east hokkaido prior to slaughter and 51.1% of 94 imported cattle slaughtered immediately after quarantine check. based on the morphology of the cyst wall and the establishment of infection in experime ... | 1994 | 7948391 |
| preparation and applicability of sarcocystis cruzi antigens and their anti-s. cruzi rabbit sera for serodiagnosis of bovine sarcocystosis. | sarcocystis cruzi antigens and their antisera were prepared for the seroimmunological diagnosis of bovine sarcocystosis. fresh s. cruzi cysts directly removed from cardial muscle of slaughtered cattle were digested with trypsin to release bradyzoites. by twelve cycles of freezing at-22 degrees c and thawing at 37 degrees c, bradyzoites were let to leach out soluble material. the soluble antigens were inoculated four times to rabbits at a dose of 343 micrograms protein and anti-s. cruzi sera were ... | 1994 | 7948400 |
| experimental infection of raccoon dogs with sarcocystis cruzi and s. miescheriana. | raccoon dogs were successfully infected with sarcocystis cruzi and s. miescheriana by oral inoculation of infected cardiac muscle of cattle and pigs slaughtered in saitama and okinawa prefectures, respectively. oocysts and sporocysts passed by raccoon dogs were similar in measurements and morphological features as reported of s. cruzi and s. miescheriana respectively. the prepatent and patent periods of both s. cruzi and s. miescheriana were 9 and 66-72 days respectively in raccoon dogs. | 1994 | 7999889 |
| use of proteinase k in the excystation of sarcocystis cruzi sporocysts for in vitro culture and dna extraction. | proteinase k was used for the cleaning of sarcocystis cruzi (apicomplexa) sporocysts prior to excystation. bovine pulmonary endothelial cell cultures inoculated with the excysted sporozoites remained free of bacterial contamination for the duration of the experiment and had high yields of merozoites. the excysted sporozoites also yielded genomic dna that could be labelled efficiently with 32p datp by the random priming method. | 1994 | 8030188 |
| serological evidence implicating neospora species as a cause of abortion in british cattle. | by means of an immunofluorescence antibody test (ifat), using in vitro cultured parasites as antigen, antibodies to neospora species at titres > or = 1/1280 were found in 11 of 120 scottish cattle that had recently aborted but in only one of 97 cattle from herds in which there had been no recent abortions (p < 0.01). the specificity of the antibodies was confirmed by the lack of cross reactivity between samples with high titres to neospora and toxoplasma antigen in a direct agglutination test, a ... | 1994 | 8036769 |
| oligonucleotide probes complementary to variable regions of 18s rrna from sarcocystis species. | sarcocystis is a large genus of cyst-forming coccidian parasites in the phylum apicomplexa (protista). stable rna was extracted from cystozoites of sarcocystis cruzi, s. tenella, s. fusiformis, s. gigantea and toxoplasma gondii. the partial sequences of the small sub-unit ribosomal rna (18s rrna) were determined by direct rna sequencing with reverse transcriptase. the rrna sequences were computer aligned with the published partial sequence of t. gondii, and three oligonucleotides complementary t ... | 1993 | 8145778 |
| evaluation of a serological test system for the diagnosis of sarcocystis cruzi infection in cattle using s. cruzi merozoite antigen. | fifty serum samples were examined by enzyme-linked immunosorbent assay (elisa) for antibodies directed against crude antigens isolated from cystozoites and merozoites of sarcocystis cruzi and sarcocystis tenella/sarcocystis arieticanis and merozoites of toxoplasma gondii. of these 50 samples, 25 were from cattle originating from an area where the prevalence of s. cruzi was found to be very low (9%) and in which no cystozoites were detected, and 25 were from cattle which were found by a digestion ... | 1994 | 8171820 |
| chronic sarcocystis infections in slaughtered cattle. | parasitological, histopathological and immunohistochemical examinations were carried out on three slaughtered cattle with many nodules in all the striated muscles. at necropsy, many yellowish green rice-grain sized nodules including cheesy contents were observed in all the striated muscles. histopathologically the nodules were granuloma principally consisting of eosinophiles. no sarcocystis cysts nor bradyzoites were found in the nodules, but intact sarcocysts were found in the normal tissues su ... | 1993 | 8286527 |
| sensitive and specific polymerase chain reaction detection of toxoplasma gondii for veterinary and medical diagnosis. | a polymerase chain reaction (pcr) method was developed for the detection of toxoplasma gondii. a universal- and a t. gondii-specific primer was used to amplify a region of the small subunit ribosomal rna gene. this approach allows for a theoretical detection limit of 0.01 zoite of t. gondii per sample assayed. experiments showed that this pcr method could detect 0.1 pg of t. gondii dna, which represents about one organism. polymerase chain reaction tests using dnas of cat, dog, swine, cattle, hu ... | 1993 | 8431804 |
| on the morphological diagnostics and host specificity of the sarcocystis species of some domesticated and wild bovini (cattle, banteng and bison). | sarcocysts of sarcocystis cruzi, s. hominis and s. hirsuta were described and compared by means of light (lm) and transmission electron microscopy (tem) from cattle, bantengs (born in a zoo) and bisons (born in zoo). no morphological differences were observed in the three forms of sarcocysts from each of the three host species. the morphological criteria of the three species were discussed and, partly, newly defined. s. cruzi can easily be distinguished from the other two species. it shows 6-13 ... | 1995 | 8541889 |
| immunodiagnosis of taenia saginata in cattle using hydrophobic antigens from t. hydatigena metacestode cyst fluid. | hydrophobic fractions isolated from cyst fluid of taenia hydatigena metacestodes, obtained from naturally infected goats from tanzania, were used in an indirect elisa and in immunoblot (western or dot blot) procedures. the use of the most hydrophobic fractions of the cyst fluid proved to result in a better diagnostic discrimination in elisa than the more hydrophilic fractions. best diagnostic ratio between responses from t. saginata infected and non-infected cattle was achieved when using horse- ... | 1995 | 8541896 |
| identification of opossums (didelphis virginiana) as the putative definitive host of sarcocystis neurona. | sarcocystis neurona is an apicomplexan that causes equine protozoal myeloencephalitis (epm) in north and south america. horses appear to be an aberrant host, because the merozoites continually divide in the central nervous system, without encysting. the natural host species has not previously been identified. the small subunit ribosomal rna (ssurna) gene of s. neurona was compared to those of sarcocystis muris, sarcocystis cruzi, toxoplasma gondii, and cryptosporidium parvum to identify a unique ... | 1995 | 8544064 |
| evaluation of commercial enzyme immunoassay (eia) and immunofluorescent antibody (fa) test kits for detection of cryptosporidium oocysts of species other than cryptosporidium parvum. | a commercial enzyme immunoassay (eia) (prospect rapid assay), a direct immunofluorescence antibody (ifa) test for stool testing (merifluor cryptosporidium/giardia), and an indirect ifa test for environmental testing (hydrofluor-combo cryptosporidium/giardia) were evaluated for detection of low public health risk cryptosporidium oocyst isolates, and for c. parvum oocyst isolates from human and bovine feces that represent a high public health risk. there was no cross-reactivity of eia with ova of ... | 1996 | 8600765 |
| systemic infection with an unidentified toxoplasma-like protozoan in a neonatal lichtenstein's hartebeest (sigmoceros lichtensteinii). | an unidentified toxoplasma gondii-like disseminated infection was found in tissues of a 2-day-old hartebeest. protozoal tachyzoites were about 3.5 x 1.7 microns, had electron-dense rhoptries, and divided by endodyogeny. the organism did not stain with polyclonol antibodies to t. gondii, neospora caninum, or sarcocystis cruzi. | 1996 | 8636866 |
| use of genomic dna probes for the diagnosis of acute sarcocystosis in experimentally infected cattle. | two clones of 1.4 and 4.33 kilobase pairs (kbp) dna inserts, were selected from a sarcocystis cruzi sporozoite genomic library constructed in bacteriophage lambda gt10. these clones strongly hybridized with sporozoite and merozoite dna and were evaluated as probes for detection of merozoite dna in clinical samples. of five calves in the experiment, four were each orally dosed with approximately 200,000 s. cruzi sporocysts; one calf served as non-infected control. subsequently, blood was collecte ... | 1996 | 8638397 |
| evaluation of two neospora caninum recombinant antigens for use in an enzyme-linked immunosorbent assay for the diagnosis of bovine neosporosis. | neospora caninum is a recently described apicomplexan parasite which causes paralysis and death in dogs. neospora parasites also cause abortion and neonatal morbidity in cattle, sheep, goats, and horses, and neosporosis is emerging as an important cause of bovine abortion worldwide. the purpose of this study was to identify n. caninum cdna clones encoding antigens that would be useful for the immunodiagnosis of bovine neosporosis. two n. caninum tachyzoite cdna clones expressing antigens that we ... | 1996 | 8705668 |
| toxicity and properties of the extract from sarcocystis cruzi cysts. | the extract from sarcocystis cruzi cysts in bovine muscle was subcutaneously injected to mice, guinea pigs, chickens, and rabbits to detect its toxicity. only rabbits showed reactions after administration of the extract at a dose of 25 micrograms. the main clinical signs of the rabbits were depression, reduction in body temperature and intermittent diarrhea and the hematological findings observed were elevation in wbc, rbc, pcv, tp, bun, ast, aut and creatinine values and reduction in glucose, k ... | 1995 | 8720045 |
| serological diagnosis of bovine neosporosis by neospora caninum monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay. | neospora caninum, a protozoan parasite closely related to toxoplasma gondii, causes abortion and congenital infection in cattle. to investigate specific methods of antemortem diagnosis, the antibody responses of infected cows were evaluated by immunoblot assay and competitive inhibition enzyme-linked immunosorbent assay (ci-elisa) by using a monoclonal antibody (mab), mab 4a4-2, against n. caninum tachyzoites. mab 4a4-2 bound diffusely to the exterior surface of n. caninum tachyzoites and recogn ... | 1996 | 8735092 |
| collagen cross-reactive antigen of sarcocystis cruzi. | collagen cross-reactive antigenic substance(s) in sarcocystis cruzi cysts were examined with immunologic techniques using anti-bovine collagen type-specific, but non-species-specific, antibodies. by immunoperoxidase test, anti-bovine collagen type-specific, but non-species-specific, antibodies. by immunoperoxidase test, anti-bovine type iv collagen antibody showed higher reactivity to the cysts than other antibodies tested. cyst wall rupture was induced by collagenase treatment and digestion was ... | 1996 | 8792577 |
| studies on pathogenesis, tissue infection and congenital transmission in cows experimentally infected with sarcocystis cruzi by various routes. | a group of nine cows, naturally infected with sarcocystis, were challenged with sarcocystis cruzi: three intrarumenally with sporocysts, two intrarumenally with water (controls), two intravenously with merozoites grown in vitro and two intravenously with saline solution (controls). the animals intrarumenally challenged with sporocysts developed acute sarcocystiosis and produced stillborn calves, whereas those intravenously challenged with merozoites suffered from subclinical sarcocystiosis with ... | 1996 | 8893486 |
| on the diagnostics of sarcocystis in cattle: sarcocysts of a species unusual for bos taurus in a dwarf zebu. | sarcocysts from the musculature of a dwarf zebu born in a german zoo are described by light and transmission electron microscopy. in addition to the three named bovine sarcocystis species (sarcocystis cruzi, s. hominis, and s. hirsuta) a fourth species was found which was not previously reported from cattle. it mostly resembles sarcocystis gracilis rátz, 1909 from roe deer. | 1996 | 8988553 |
| development of a polymerase chain reaction assay for the diagnosis of neosporosis using the neospora caninum 14-3-3 gene. | neospora caninum is a recently described apicomplexan parasite which causes neuromuscular disease in dogs, and abortion and neonatal morbidity in cattle, sheep and horses. morphological similarities and serological cross-reactivity between n. caninum and the closely related parasite toxoplasma gondii, have resulted in the frequent misdiagnosis of neosporosis as toxoplasmosis. this report describes the isolation and characterization of an n. caninum cdna clone encoding a 14-3-3 protein homologue. ... | 1996 | 8992315 |
| viability of the sporocysts of sarcocystis cruzi after exposure to different temperatures and relative humidities. | the effect of temperature and relative humidity (rh) on the survival of sporocysts of s. cruzi were investigated in vitro. under all experimental conditions (temperature of 4 degrees c, 37 degrees c, or room temperature; rh of 18%, 75%, or 100%) some sporozoites retained their viability to excyst for at least 90 days. the best conditions for survival were 4 degrees c at 100% rh (more than 240 days) and 37 degrees c at 18% rh (more than 180 days). sporocysts maintained at room temperature at all ... | 1996 | 9017863 |
| characterization of a swedish bovine isolate of neospora caninum. | the brain of a stillborn calf, seropositive to neospora caninum and born to a seropositive cow, was homogenized and cultured on vero cells, where growth of neospora-like tachyzoites was detected after 8 weeks. the ultrastructural features of the new isolate (nc-sweb1) corresponded to those of previously published neospora isolates. in indirect immunofluorescence tests, antigens on nc-sweb1 tachyzoites were recognized by antibodies raised to a canine n. caninum isolate (nc-1) but not by antibodie ... | 1997 | 9089715 |
| novel elisa for detection of neospora-specific antibodies in cattle. | an enzyme-linked immunosorbent assay (elisa) to detect antibodies to neospora species in cattle was developed. whole formalin-fixed neospora caninum (nc-liverpool) tachyzoites were used as antigen and a monoclonal antibody to bovine immunoglobulin light chain and an anti-mouse horseradish peroxidase conjugate were used to reveal bound antibody. a panel of 46 sera, negative by the immunofluorescent antibody test (ifat), were used in the elisa at a serum dilution of 1:500 to calculate the negative ... | 1997 | 9106971 |
| immunohistochemical diagnosis of toxoplasma gondii: potential for cross-reactivity with neospora caninum. | a monoclonal antibody (mab 3f5) was compared with a commercial polyclonal antibody for specificity for toxoplasma gondii and cross-reactivity with neospora caninum in paraffin-embedded tissue sections. both antibody preparations reliably recognized t. gondii tachyzoites (rh isolate) in animal tissues but did not always label tissue cysts (me-49 isolate). the commercial antibody strongly cross-reacted with n. caninum tachyzoites, but mab 3f5 did not when the immunoperoxidase, immunofluorescence, ... | 1997 | 9194824 |
| meningoencephalitis in capercaillie (tetrao urogallus l.) caused by a sarcocystis-like organism. | a nonsuppurative meningoencephalitis, previously presumed to be toxoplasmosis, was found in 53 capercaillies (tetrao urogallus l.) examined at necropsy at the national veterinary institute, uppsala, sweden, between 1966 and 1985. pronounced meningitis and encephalitis with perivascular cuffs of mononuclear inflammatory cells as well as focal gliosis were prominent histopathologic findings. protozoa were frequently associated with these lesions. ultrastructurally, the protozoa appeared to divide ... | 1997 | 9365940 |
| in vitro isolation and characterisation of a bovine neospora species in japan. | eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37 degrees c with 5 per cent co2. neospora tachyzoites were observed in one cell 49 days after inoculation. the isolated parasite (jpa1) was morphologically identical to the previously reported bovine neospora species (bpa1) and confirmed by its strong antigenic r ... | 1997 | 9368961 |
| class-specific antibody responses in cattle following experimental challenge with sporocysts or merozoites of sarcocystis cruzi. | an elisa using antigen produced from merozoites of sarcocystis cruzi was developed to monitor specific igm and igg antibody, following challenge of cattle with either merozoites or sporocysts of s. cruzi. this assay was compared with an elisa using antigen produced from the cystozoite stage of the parasite. both elisas were able to detect significant increases in levels of circulating igm and igg antibodies against sarcocystis in all challenged cows; however, the magnitude of the titres was grea ... | 1997 | 9404838 |
| excystation rates and infectivity of sporocysts of sarcocystis cruzi exposed to different treatments and storages. | the effect of some chemical and anatomical factors on the excystability and infectivity in cell culture of sporocysts of sarcocystis cruzi was investigated. a significantly (p < 0.001) higher excystation rate (er) occurred when sodium bicarbonate was added to the excysting fluid at concentrations between 0.075 and 0.3 m. sporocysts collected from different dogs had different er. in contrast, although not uniformly distributed along the intestinal lumen, the sporocysts collected from the differen ... | 1997 | 9477488 |
| characterization of bovine sarcocystis species by analysis of their 18s ribosomal dna sequences. | two sarcocystis hirsuta isolates from 2 cattle (bos taurus), 2 sarcocystis hominis-like isolates from 2 cattle, 1 each sarcocystis hominis-like isolate from a zoo-born dwarf zebu (b. taurus) and from a zoo-born bison (bison bison), and 1 each sarcocystis cruzi isolate from the dwarf zebu and the bison have been characterized by comparing the directly sequenced polymerase chain reaction products of their 18s rrna genes. sequences of 2 different isolates from the same species always showed a very ... | 1998 | 9488337 |
| sarcocystis-associated encephalitis and myocarditis in a wild turkey (meleagris gallopavo). | a sarcocystis-like organism was associated with encephalitis and myocarditis in an ataxic, emaciated adult male turkey (meleagris gallopavo) from gilmer county, west virginia. protozoal schizonts and merozoites were associated with areas of inflammation and occasionally necrosis in both the heart and the brain. the organisms divided by endopolygeny and stained positively with anti-sarcocystis cruzi serum in an immunohistochemical test. | 1998 | 9645885 |
| a survey of bovine, bubaline and swine sarcocystosis in the philippines. | in a survey conducted from january to december, 1994, muscle tissues in 12 out of 22 slaughtered carabaos ages 8-17 years old and obtained from the food terminal inc, abbatoir in laguna showed numerous white and creamy elliptic-shaped soft bodied macrocysts in the throat muscles. microscopic examination of the throat and cardiac muscle tissues revealed the presence of fusiform-shaped microcysts. our observations are consistent with previous reports incriminating sarcocystis fusiformis as the mos ... | 1997 | 9656372 |
| changes in somatotropic axis response and body composition during growth hormone administration in progressive cachectic parasitism. | a multistage protozoan parasitic disease was used as a cachexia model to study the effects of daily administration of bovine growth hormone (gh) on endocrine and body composition changes of young calves from the onset of the acute phase response (apr). male calves averaging 127.5 +/- 2.0 kg body weight were assigned to control, ad libitum fed, noninfected (c); ad libitum fed, infected (250,000 oocysts sarcocystis cruzi, per os, i); noninfected, pair-fed (pf) to matched i-treatment calves and the ... | 1998 | 9673456 |
| blood clotting disorders during experimental sarcocystiosis in calves. | the effects of experimental infection of calves with sarcocystis cruzi on the blood coagulation cascade were investigated. calves were inoculated orally with 1 x 10(5) sporocysts (group s1, n = 6) or with 5 x 10(5) sporocysts of s. cruzi (group s2, n = 3). a group of eight calves served as non-infected controls (group c). the animals were bled once during the first 4 weeks of infection and twice a week thereafter until day 40 p.i. the following parameters were measured: activated partial thrombo ... | 1998 | 9762563 |
| a multiple antigen elisa to detect neospora-specific antibodies in bovine sera, bovine foetal fluids, ovine and caprine sera. | neospora caninum is a cyst-forming coccidian parasite recently identified as a cause of abortion in cattle. the epidemiology of neosporosis is poorly understood, partly because accurate diagnosis of infection is difficult. in this paper we describe the development of a multiple antigen-based enzyme-linked immunosorbent assay (elisa) to detect antibodies to n. caninum in sera from cattle, sheep and goats as well as from bovine foetal fluids. a water-soluble fraction (wsf) of sonicated nc-1 strain ... | 1998 | 9777723 |
| endocrine modulation of physiological responses to catabolic disease. | disease or endotoxemia alters the plasma concentrations of anabolic hormones, particularly growth hormone (gh) and insulin-like growth-factor i (igf-i). in general, these hormones are inhibited during the catabolic disease state. a hypothesis has evolved that anabolic hormones might be useful in patients' recovery under these and other catabolic circumstances. the treatment of cattle with gh has provided significant improvement in the physiological response of the animals to the subsequent injec ... | 1998 | 9785046 |
| serological differences in neospora caninum-associated epidemic and endemic abortions. | a sandwich enzyme-linked immunosorbent assay (elisa) for the sensitive and specific detection of bovine antibodies to neospora caninum was developed and evaluated using sera from cattle experimentally infected with n. caninum, toxoplasma gondii, sarcocystis cruzi, sarcocystis hominis, sarcocystis hirsuta, eimeria bovis, cryptosporidium parvum, babesia divergens, and field sera from naturally exposed animals. field sera were classified using a gold standard that included the results from an indir ... | 1999 | 10461950 |
| induction of tumor necrosis factor alpha and nitric oxide in rabbits inoculated with a cyst extract of sarcocystis cruzi. | rabbits develop a toxic reaction similar to endotoxemia following inoculation with a sarcocystis cruzi cyst extract. to analyze the pathophysiology of the reaction, serum tumor necrosis factor alpha (tnfalpha), nitric oxide (no) and lipid-lipoprotein profiles were investigated in rabbits given the cyst extract and lipopolysaccharide (lps) by subcutaneous inoculation. in animals given the cyst extract, overproduction of tnfalpha was detected, together with an increase in no. the animals developed ... | 1999 | 10488726 |
| underlying disease stress augments plasma and tissue adrenomedullin (am) responses to endotoxin: colocalized increases in am and inducible nitric oxide synthase within pancreatic islets. | rapid onset metabolic impairments accompany the initiation of the acute phase response to many disease stresses, whereas more chronic metabolic perturbations may prolong the recovery period. in the present experiment the application of a mild endotoxin challenge [lipopolysaccharide (lps)] alone or additive to a chronic subclinical parasitic infection (sarcocystis cruzi; lps + pi) in calves was used as a model to investigate and define a dynamic axis coordinated between adrenomedullin (am) and ni ... | 1999 | 10537172 |
| growth performance, meat quality and activities of glycolytic enzymes in the blood and muscle tissue of calves infected with sarcocystis cruzi. | growth performance and the pattern of glycolytic enzymes in the blood plasma were assessed during experimental sarcocystis cruzi infection (1 x 10(5) sporocysts per calf) in six calves; five calves served as noninfected controls. at slaughter (68 or 88 days post infection), carcass weight, dressing percentages and several parameters of meat quality (ph, color brightness, rigor, water absorbing capacity, water binding capacity) were recorded. moreover, enzyme activities were measured in muscle ho ... | 2000 | 10681018 |
| improvement of western blot test specificity for detecting equine serum antibodies to sarcocystis neurona. | equine protozoal myeloencephalitis (epm) is a neurological disease of horses and ponies caused by the apicomplexan protozoan parasite sarcocystis neurona. the purposes of this study were to develop the most stringent criteria possible for a positive test result, to estimate the sensitivity and specificity of the epm western blot antibody test, and to assess the ability of bovine antibodies to sarcocystis cruzi to act as a blocking agent to minimize false-positive results in the western blot test ... | 2000 | 10690772 |
| prevalence of sarcocystis spp. cysts in japanese and imported beef (loin: musculus longissimus). | a survey was carried out to investigate the occurrence of sarcocystis infection in the loin (musculus longissimus) of japanese and imported beef. in all, the muscle tissue of 482 samples were examined by histological method. the prevalence of sarcocystis unspecified species cysts was lower in japanese beef (total 6.31%: 0% in holstein castrated, 12.96% in holstein milk cow, 3.33% in japanese shorthorn and 11.58% in japanese black cattle) than in beef imported from america (36.78%) or australia ( ... | 1999 | 11269330 |
| specific detection of neospora caninum oocysts in fecal samples from experimentally-infected dogs using the polymerase chain reaction. | neospora caninum oocysts, passed in the feces of a definitive host (dog), were isolated, and genomic dna was extracted. a polymerase cahin reaction (pcr) targeting the n. caninum-specific nc 5 genomic sequence was performed using the isolated dna. a synthesized competitor molecule containing part of the nc 5 sequence was included in the assay as a check against false-negative pcr results and to quantify n. caninum oocyst dna in fecal samples. a standard curve of the ratio of fluorescence intensi ... | 2001 | 11318571 |