| [the ferret (putorius putorius furo), an additional final host of sarcocystis muris (author's transl)]. | two ferrets were fed mice experimentally infected with sarcocystis muris. after 7 days they excreted with their faeces for 9 days sporocysts which were morphologically indistinguishable from s. muris sporocysts. five mice which each received 70 of these sporocysts orally developed macroscopically visible cysts of s. muris in their musculature after 4 months. | 1979 | 107667 |
| a study on the life cycle of sarcocystis muris. | | 1978 | 110897 |
| ultrastructure of the cyst of sarcocystis muris. | cysts of sarcocystis muris develop within muscle cells and each is bounded by a parasitophorous vacuole membrane. closely spaced spherical blebs formed from this membrane extend into the muscle cell cytoplasm. a dense substance fills the cavity of the bleb and occupies the vacuolar space immediately adjacent to the membrane. the remainder of the vacuole is filled with a moderately dense matrix within which the parasites develop. at 40 days after infection only metrocytes are present, characteriz ... | 1977 | 407345 |
| separation of sarcocystis muris and isospora felis in mice used by powell and mccarley (1975) in studies on the life cycle of s. muris. | | 1978 | 415126 |
| cockroaches as vectors of sarcocystis muris and of other coccidia in the laboratory. | to assess the roles of the german cockroach (blatella germanica) and the american cockroach (periplaneta americana) in the transmission of sarcocystis muris and of 3 other coccidia of cats-toxoplasma gondii, isospora felis, and isospora rivolta, cockroaches exposed to feces containing these coccidia were periodically fed to mice, as was a portion of the fecal matter. sarcocystis muris sporocysts, which in feces remained infectious for at least 20 days, were also transmitted to mice by p. america ... | 1978 | 417163 |
| who named sarcocystis muris? | | 1978 | 418746 |
| a murine sarcocystis that causes an isospora-like infection in cats. | sarcocysts similar to those of sarcocystis muris were found in mice from the iowa state university mouse genetics laboratory. muscle tissue from these mice was fed to 8 cats. five cats developed an isosporan infection with oocysts most like those of isospora felis. sporulated oocysts from cat feces were fed to mice. in about 100 days mice developed sarcocysts that were visible to the unaided eye. three cats were fed muscle tissue from the experimentally infected mice, and all cats developed an i ... | 1975 | 810560 |
| recognition of cyclic transmission of sarcocystis muris by cats. | sarcocystis muris, obtained from laboratory and house mice, was transmitted experimentally to cats. when cysts in the skeletal muscle of mice were ingested by cats, bradyzoites developed directly into gametes in the intestinal mucosa of the cats. after five to 11 days, infectious sporulated sporocysts (8.5 x 10.3 mum) were shed by cats for up to three months. after ingestion of the sporocysts, s. muris multiplied first in the livers and then in the skeletal muscle of mice. cysts in skeletal musc ... | 1976 | 816973 |
| the epidemiology of sarcocystis muris in rodents from egypt. | | 1976 | 828921 |
| a comparison of sporozoite and cyst merozoite surface proteins of sarcocystis. | surface labeling of sarcocystis muris and s. suicanis sporozoites with n-hydroxysuccinimide biotin led to the detection of major membrane proteins with relative molecular weights of 29 and 30 kda, respectively. immunoblots of sarcocystis sporozoite proteins probed with sera from infected hosts or with polyclonal monospecific antibodies generated against membrane antigens of cyst merozoites (noncorresponding stages) showed cross-reactivity between the two developmental stages (cyst merozoites and ... | 1992 | 1379712 |
| development of a model ribosomal rna hybridization assay for the detection of sarcocystis and other coccidia. | two regions of the primary structure of the small subunit rrna of sarcocystis muris bradyzoites were compared with nucleotide sequences of s. gigantea, toxoplasma gondii, plasmodium berghei and mus musculus and used to design genus- and species-specific probes for the detection and identification of coccidia. total cellular rna of purified s. muris, s. cruzi, t. gondii and eimeria nieschulzi and coccidia-infected tissues of mouse, ox, sheep and pig, were assayed using twenty-base oligomers label ... | 1992 | 1423056 |
| microneme secretion in coccidia: confocal laser scanning and electron microscope study of sarcocystis muris in cell culture. | a monoclonal antibody (mcab) raised against a subcellular fraction of sarcocystis muris cystozoites was used to localize microneme antigens before, during and after invasion of cultured cells. the mcab recognized a 20 and 22 kda protein under reducing and non-reducing conditions on western blots and localized an antigen in cystozoites in the apical part of the parasites. confocal laser scanning microscopy of invading cystozoites revealed the secretion of a microneme antigen at the apical tip of ... | 1992 | 1493806 |
| cloning and expression in escherichia coli of cdnas encoding a 31-kilodalton surface antigen of sarcocystis muris. | using polyadenylated rna isolated from sarcocystis muris cyst merozoites, we have constructed a cdna library in the expression vector lambda zap. immunoscreening with monoclonal and polyclonal antibodies directed against a 31-kda surface antigen of s. muris [1] yielded a number of clones with insert sizes ranging between 1.1 kb and 1.3 kb. an additional clone with an insert length of 1.55 kb was isolated by screening with a labeled dna probe derived from one of the cdna clones. the cdna sequence ... | 1992 | 1501635 |
| the nucleotide sequence of the small subunit ribosomal rna gene from symbiodinium pilosum, a symbiotic dinoflagellate. | the complete sequence of the small subunit ribosomal rna (ssu rrna) gene was determined for the symbiotic dinoflagellate symbiodinium pilosum. this sequence was compared with sequences from two other dinoflagellates (prorocentrum micans and crypthecodinium cohnii), five apicomplexa, five ciliata, five other eukaryotes and one archaebacterium. the corresponding structurally conserved regions of the molecule were used to determine which portions of the sequences could be unambiguously aligned. phy ... | 1992 | 1525867 |
| [the cytochemical demonstration of glycoproteins and glycolipids in sarcocystis muris sarcocysts]. | an electron cytochemical study of glycoproteins and glycolipids was made for the mature sarcocysts of sarcocystis muris. glycoprotein structures as branched fibrilles were seen on the surface of the sarcocyst wall. the fibrillar and granular glycoprotein structures were found in the ground substance of sarcocysts near the cyst wall and in the septae. in the plasmalemma of two types of cyst stages (merozoites and intermediate cells), glycoprotein fibrillar structures were revealed connecting thes ... | 1992 | 1641911 |
| dna measurements and ploidy determination of different stages in the life cycle of sarcocystis muris. | the dna contents of different stages within the life cycle of sarcocystis muris were measured cytophotometrically using dna-specific feulgen staining. stages of gamogony were obtained by the transfer of isolated cyst merozoites into cat kidney-cell cultures. for calculation of absolute dna contents, the amounts of dna in the parasites were compared with those in chicken erythrocytes, which are known. the measurements revealed that all investigated stages of s. muris contained haploid dna except ... | 1990 | 1701251 |
| detection of species-specific and cross-reactive epitopes in sarcocystis cystozoites by monoclonal antibodies. | a total of 24 hybridoma cell lines producing monoclonal antibodies (mabs) to sarcocystis muris were established from 3 fusions performed on mice that had been immunized with total protein derived from cystozoites. in all, 19 mabs were of the igg1 isotype, 3 were of the igg2a isotype, and 2 were of the igg3 isotype. the reactivity of the mabs with homologous and heterologous antigens was examined by an indirect fluorescent antibody test, an enzyme-linked immunosorbent assay (elisa), and a dot eli ... | 1991 | 1710803 |
| antigenic characterisation of monoclonal antibodies against sarcocystis muris by western blotting and immuno-electron microscopy. | the antigenic specificities of 13 monoclonal antibodies (mabs) raised against sarcocystis muris cystozoites were examined by western blotting and immuno-electron microscopy against homologous s. muris and heterologous s. gigantea, s. tenella, s. arieticanis, s. capracanis, s. miescheriana and toxoplasma gondii antigens. four mabs reacted in western blots against s. muris antigens: sm-4 and -17 recognized single antigenic bands (31,000 and 34,000 mw, respectively) and sm-2 and -3 reacted against ... | 1991 | 1710804 |
| sarcocystis muris (apicomplexa): a thiol protease from the dense granules. | homogenates of sarcocystis muris merozoites (cyst form) and the subcellular fraction of dense granules were assayed for protease activity with substrate-impregnated sds-polyacrylamide gels. four acidic and several basic proteases were detected in the merozoites. one of the basic proteases was further characterized as a thiol protease (ec 3.4.22). the activity of this protease was enriched in the dense granule fraction. | 1992 | 1730265 |
| [the cytochemical study of polysaccharides in coccidia of the genus sarcocystis. i. sulfated glycosaminoglycans in sarcocystis muris sarcocysts]. | an electron microscope study of sulfatized glycosaminoglycans (sg) was made for cyst stages of s. muris. the polysaccharides were detected in the submembranous and subwall layers of the sarcocysts, in addition to the ground substance and septae. moreover sg were discovered in the cyst stages themselves--metrocytes, intermediate cells and merozoites (gamonts). sg were discernible as electron dark spots in vacuoles of the metrocytes. sg shaped as granules were scattered in the cytoplasm of both in ... | 1991 | 1801380 |
| effect of tryptic or peptic digestion or mechanical isolation on the extraction of proteins, antigens, and ribonucleic acids from sarcocystis muris bradyzoites. | various methods have been used for the isolation of bradyzoites of sarcocystis species. using sarcocystis muris as a model, the effect of 3 methods, trypsin digestion, pepsin digestion, and mechanical isolation, on the subsequent extraction of s. muris proteins, antigens, and rna was examined. although some quantitative differences were found among the proteins, antigens, and rna extracted after the 3 isolation methods, qualitative differences were not evident. the overall isoelectric focusing p ... | 1991 | 1826327 |
| monoclonal antibodies identify micronemes and a new population of cytoplasmic granules cross-reacting with micronemes of cystozoites of sarcocystis muris. | micronemes of cystozoites of sarcocystis muris were isolated after subcellular fractionation and used for immunization of balb/c mice. after spleen cells of immunized mice were fused with sp20 myeloma cells, ten different monoclonal antibodies (mabs) were isolated. these antibodies reacted with antigens whose molecular weight ranged from 16 to greater than 90 kda. six mabs recognized granules of 150-400 nm that were located in the vicinity of the golgi complex but were not identical with dense g ... | 1991 | 1899725 |
| biochemical and immunological characterization of major surface antigens of sarcocystis muris and s. suicanis cyst merozoites. | surface proteins of sarcocystis cyst merozoites were labeled by biotinylation or radioiodination and identified on western blots after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the major labeled proteins of s. muris and s. suicanis have relative molecular masses of 31 and 33 kda, respectively. immunoblots performed with the 31-kda protein and sera of experimentally infected mice or with monoclonal antibodies to s. muris revealed that this protein is immunogenic. indir ... | 1991 | 1904582 |
| ribosomal rna sequences of sarcocystis muris, theileria annulata and crypthecodinium cohnii reveal evolutionary relationships among apicomplexans, dinoflagellates, and ciliates. | sarcocystis muris is a coccidium with a two-host life cycle involving the domestic cat and the mouse, mus musculus. s. muris and theileria annulata belong to the phylum apicomplexa, but the latter organism is a tick-borne protozoon in the subclass piroplasmea and causes tropical theileriosis in cattle. the small-subunit ribosomal rna (16s-like rrna) coding regions of these organisms as well as that of the free living dinoflagellate crypthecodinium cohnii were amplified using polymerase chain rea ... | 1991 | 1904987 |
| separation of antigens from sarcocystis species using chromatofocusing. | crude antigen preparations from bradyzoites of sarcocystis species exhibit a high degree of cross-reactivity with antisera against heterologous sarcocystis species, preventing the development of a species-specific immunological test for sarcocystiosis. in this study, we fractionated bradyzoite-derived protein extracts from sarcocystis tenella, sarcocystis arieticanis, sarcocystis gigantea, and sarcocystis muris by chromatofocusing and obtained distinct protein elution profiles for each species. ... | 1991 | 1919920 |
| evolutionary relationships of avian eimeria species among other apicomplexan protozoa: monophyly of the apicomplexa is supported. | direct, reverse transcriptase-mediated, partial sequencing of the small-subunit (16s-like) ribosomal rna (srrna) of eimeria tenella and e. acervulina was performed. sequences were aligned by eye with six previously published, partial or complete srrna sequences of apicomplexan protists (plasmodium berghei, theileria annulata, cryptosporidium sp., toxoplasma gondii, sarcocystis muris, and s. gigantea). six eukaryotic protists (a slime mold, a yeast, two dinoflagellates, and two ciliates) acted as ... | 1991 | 2072862 |
| production and characterisation of monoclonal antibodies for species diagnosis of sarcosporidia. | monoclonal antibodies were raised against cystozoites of sarcocystis muris and characterised. twelve monoclonal antibodies reacted in the elisa, dot-elisa and ifat only with homologous antigen. the other twelve showed cross reactions of various degrees with cystozoites of s. gigantea, s. tenella, s. arieticanis, s. capracanis, s. miescheriana and s. suihominis. proteins of s. arieticanis, s. tenella, s. gigantea, s. capracanis, s. muris and toxoplasma gondii were compared by isoelectric focusing ... | 1990 | 2132150 |
| monoclonal antibodies against cystozoites of sarcocystis muris (protozoa, apicomplexa). | | 1987 | 2447581 |
| immunosuppression in sarcocystis muris-infected mice: evidence for suppression of antibody and cell-mediated responses to a heterologous antigen. | mice infected with sarcocystis muris showed a significant reduction in plaque-forming cells (pfc) and delayed-type hypersensitivity (dhs) responses to an unrelated protein antigen, bovine gamma-globulin, when compared with uninfected controls. this immunosuppression was observed only when infection preceded immunization or when mice were immunized concurrently with infection, suggesting that suppression induced by murine sarcocystosis affected the induction and/or the differentiation of antigen- ... | 1988 | 2485090 |
| characterization and immunolocalization of the protein contents of micronemes of sarcocystis muris cystozoites (protozoa, apicomplexa). | affinity-purified polyclonal antibodies generated against micronemes obtained by the subcellular fractionation of sarcocystis muris contain two major proteins of 16 and 17 kda. antibodies cross-react with microneme antigen of s. tenella and s. cuniculi but not with the s. sp. of wild boar (sus scrofa). microneme antigens could be traced by immunoelectron microscopy in metrocytes as early as 47 days postinfection and in gamonts of s. muris in cell cultures up to 4 h after inoculation. | 1989 | 2496409 |
| in vitro and in vivo interaction between sporocysts of sarcocystis muris and mouse peritoneal macrophages. | the interaction between the sporocysts of sarcocystis muris and mouse peritoneal macrophages was studied both in vitro and in vivo in an attempt to determine whether or not resident peritoneal macrophages might effect the excystation of s. muris sporozoites from sporocysts injected intraperitoneally. sporocysts of s. muris were phagocytosed by peritoneal macrophages both in vitro and in vivo. the addition of either unheated mouse serum or fetal calf serum did not significantly alter the level of ... | 1989 | 2506690 |
| comparison of enzyme-linked immunosorbent assay and indirect fluorescent antibody test for the detection of igg antibodies to sarcocystis muris. | sarcocystis muris cystozoites were separated from host tissue after mechanical mincing and homogenization by discontinuous density gradient centrifugation using percoll. the isolated antigen, consisting of more than 95% cystozoites, was used in an enzyme-linked immunosorbent assay (elisa) and an indirect fluorescent antibody test (ifat). in the elisa, the antigen was used at a very low protein concentration of 0.44 micrograms/ml. good reproducibility of test results was achieved with different c ... | 1987 | 2965841 |
| immunoelectron microscopic demonstration of the exocytosis of dense granule contents into the secondary parasitophorous vacuole of sarcocystis muris (protozoa, apicomplexa). | merozoites of the parasitic protozoon sarcocystis muris (apicomplexa) possess three types of characteristic organelles with electron dense contents named rhoptries, micronemes, and dense granules, which are supposed to be involved in the parasite-host cell interactions during and after invasion. dense granules were purified from a merozoite homogenate by centrifugation on a sucrose density gradient. it was shown by sds polyacrylamide gel electrophoresis that they contain a major protein of 21 kd ... | 1986 | 3093233 |
| [differentiation of sarcocystis muris and s. dispersa infections of the mouse using isoelectric focusing and immune assays]. | | 1986 | 3096685 |
| [light optical and electron microscopy research on the cyst-forming coccidium sarcocystis muris]. | part of the complicated life cycle of sarcocystis muris, confined to the muscle cyst (sarcocyst), has been studied by light and electron microscopy. the early development of the sarcocyst proceeds strictly intracellularly, whereas the older and larger cysts tend to destroy the harbouring muscle cell, and since then their development seems to be intercellular rather than intracellular. three different cell types are distinguished within the growing sarcocyst of s. muris differing from each other ... | 1986 | 3105146 |
| [electron microscopic study of the process of intermediate cell division in the cyst of the coccidian sarcocystis muris]. | the intermediate cell is a third definitely outlined morpho-functional type of cells within sarcocysts, in addition to the two other well known ones--metrocytes and merozoites (fedoseenko, levit, 1979; beyer et al., 1981). the intermediate cell divides by endodyogeny, the nuclear division being accomplished by semi-closed pleuromitosis. in the dividing nuclei, centrioles and extranuclear bundle of microtubules connecting two pairs of centrioles are seen in addition to centrocones and associated ... | 1987 | 3111042 |
| comparison of dot-elisa, elisa and ifat for the detection of igg antibodies to sarcocystis muris in experimentally infected and immunized mice. | the dot enzyme-linked immunosorbent assay (dot-elisa) was used for the detection of igg antibodies to sarcocystis muris and compared with the enzyme-linked immunosorbent assay (elisa) and the indirect fluorescent antibody test (ifat). in experimentally infected mice, first positive reactions occurred in the dot-elisa between 18 and 32 days after infection (dpi), in the elisa between 18 and 49 dpi, and in the ifat between 11 and 25 dpi. maximum titers were 1:40 960 in the dot-elisa, 1:1280 in the ... | 1988 | 3144082 |
| [the final stage of division of the intermediate cell in a sarcocystis muris muscle cyst]. | at least two functions of the micropore, one of the zoite's apical organelles, have been established on examining s. muris intermediate cells. the micropore is known to function primarily as an ultracytostome for taking up the food. the newly discovered function of the micropore appears to be involved in the final step of daughter cell separation in dividing intermediate cell. the micropores seem to be the sites where daughter cells find their exit from the mother cell. in addition, the pattern ... | 1988 | 3144780 |
| cellular changes in the spleens of mice infected with sarcocystis muris. | cellular changes in the spleens of mice infected with sarcocystis muris have been studied. immunofluorescent staining for b and t cells and alpha-naphthyl acetate esterase (anae) staining for macrophages combined with histological studies revealed marked changes in the populations and distributions of all three cell types. infection was accompanied by a marked splenomegaly, attributable mainly to widespread hyperplasia of the white pulp. following infection there was an increase in the relative ... | 1988 | 3147239 |
| [development of the cyst-forming coccidian sarcocystis muris in the body of its intermediate host]. | on examining the cyst merogony of s. muris, two patterns of development were established. the one occurs in young cysts where metrocytes underwent endopolygeny, giving rise to the intermediate cells. no dividing metrocytes were seen in the older cysts. unlike, metrocyte differentiation was observed in these from 1, 2 and 3 step metrocytes to the intermediate cell state, which is the other pattern of development. an original scheme of development within the cysts of s. muris is proposed. | 1988 | 3149810 |
| structure, isolation, and protein composition of the pellicle of sarcocystis muris cystozoites (protozoa, coccidia). | the molecular organization of the sarcocystis muris cystozoite pellicle has been investigated by freeze-fracture electron microscopy and by electrophoresis of the proteins of isolated pellicles. freeze-fracture revealed a highly ordered organization of the inner membrane complex similar to the one described in other coccidian zoites. purification of pellicles was achieved by french press homogenization followed by sucrose gradient floatation. electron microscopy of the pellicle fraction demonstr ... | 1985 | 3921698 |
| invasion and early development of sarcocystis muris (apicomplexa, sarcocystidae) in tissue cultures. | ultrastructural observations on the invasion and early development of merozoites (bradyzoites) of sarcocystis muris in madin-darby canine kidney (mdck) cells are presented. invading merozoites cause the host cell plasmalemma to invaginate; they form a membrane junction (moving junction) and move into the host cell where they are enclosed in a primary parasitophorous vacuole (pv). within 30-45 min after becoming intracellular, merozoites begin to vacate the newly established primary pv and move, ... | 1985 | 3930705 |
| sarcocystis in mice inoculated with toxoplasma-like oocysts from cat feces. | sarcocysts morphologically similar to sarcocystis muris were observed in mice after inoculation with toxoplasma-like oocysts found in feces of a stray cat. cats that were fed mice infected with the oocysts shed similar oocysts in their feces. sarcocysts were found histologically in about 50 percent of mice inoculated with 40,000 or more oocysts and examined 42 days or longer after exposure. most inoculated mice developed low toxoplasma dye-test antibody titers 3 to 4 weeks after exposure, but to ... | 1973 | 4196874 |
| [immunization of mice against sarcosporidia (sarcocystis muris) with attenuated sporocysts]. | | 1982 | 6287998 |
| detection of sarcocystis antigens in the sera of experimentally-infected pigs and mice by an immunoenzymatic assay. | immunoglobulins raised against sarcocystis miescheriana and sarcocystis muris cystozoite antigens were isolated from rabbit immune sera by affinity chromatography (using cnbr-activated sepharose 4b for antigen immobilization). the specific immunoglobulins were incorporated into double-antibody sandwich immuno-enzymatic assays which were firstly quantitated and then used to detect soluble sarcocystis antigens in the sera of experimentally-infected pigs and mice. assays employing immunoglobulins a ... | 1983 | 6407180 |
| identification of surface antigens of sarcocystis muris (coccidia). | zoites of sarcocystis muris were recovered from the skeletal muscles of infected mice by trypsin digestion. extracts of zoites prepared by freeze-thaw, triton x-100 (0.1%), or a combination of the two treatments contained antigenic components. testing of these antigens by agar gel diffusion and immunoelectrophoresis against sera from infected mice showed one major precipitin band. sds-polyacrylamide-gel electrophoresis (sds-page) of the extracts revealed at least eight detectable polypeptides ra ... | 1983 | 6415269 |
| electron microscope study of host-parasite interactions of sarcocystis muris (protozoa, coccidia) in tissue culture and in vivo. | | 1984 | 6421020 |
| cross-transmission and in vitro excystation experiments with sarcocystis muris. | | 1984 | 6429308 |
| examinations on the serodiagnosis of sarcocystis infections. ii. class-specific immunoglobulin responses in mice, pigs, and sheep. | mice, pigs and sheep were experimentally infected with sarcocystis muris, s. miescheriana (syn. s. suicanis) and s. tenella (syn. s. ovicanis) respectively. enzyme-linked immunosorbent assays employing conjugates specific for the relevant host immunoglobulin classes m and g were then evaluated for use in serodiagnosing these infections. soluble antigenic extracts were prepared from cystozoites harvested from macroscopic s. muris and s. gigantea (syn. s. ovifelis) cysts and from microscopic s. mi ... | 1984 | 6431711 |
| ultrastructural comparison of cysts and zoites of toxoplasma gondii, sarcocystis muris, and hammondia hammondi in skeletal muscle of mice. | | 1980 | 6767834 |
| myofiber damage accompanying intramuscular parasitism by sarcocystis muris. | myofiber degeneration which results from sarcocystis infection exhibits a number of fine structural features suggestive of other myopathies and several well-defined fine structural features not characteristic of other myopathies. some of these fine structural features are similar to those observed in intramuscular infections of trichinella spiralis, another muscle parasite. major alterations of the myofibrillar contractile apparatus occur at the periphery of the membrane bound parasitophorous va ... | 1981 | 6784266 |
| the sarcocystis muris-infection as a model for research on the chemotherapy of acute sarcocystosis of domestic animals. | twelve anticoccidial or antimalarial drugs were tested for their efficacy against various development stages of sarcocystis muris in nmri-mice. schizogonic stages present in the liver from day 11 to 17 p.i. showed to be most sensitive to drug action. sulfaquinoxaline plus pyrimethamine, zoalene and bay g 7183 completely eliminated these stages. a strong though not 100 per cent efficacy was observed in experiments with primaquine. the other drugs tested were less (halofuginone, sulfadoxine plus t ... | 1981 | 6797157 |
| [cellular and humoral reactions in mice following experimental infections with sarcocystis muris and s. dispersa]. | | 1982 | 6808986 |
| sarcocystis and other coccidia in foxes and other wild carnivores from montana. | sarcocystis spp sporocysts were found in feces of 10.1% of 198 red foxes (vulpes vulpes), in 3.2% of 61 bobcats (lynx rufus), in 16.6% of 12 mountain lions (felis concolor), in 16.6% of 6 fisher (martes pennanti), and in none of 20 wolverines (gulo gulo), 4 mink (mustela vison), or 10 raccoons (procyon lotor). sarcocystis muris and toxoplasma gondii were not found in laboratory mice inoculated with feces of bobcats and mountain lions. | 1982 | 6816776 |
| the sag2 antigen of toxoplasma gondii and the 31-kda surface antigen of sarcocystis muris share similar sequence features. | in the search for similar cysteine distribution patterns among surface antigens of members of the phylum of apicomplexa, we found a resemblance between tgsag2, a 22-kda surface antigen of toxoplasma gondii, and the c-terminal half of a 31-kda surface antigen of sarcocystis muris, smsag1. the overall degree of similarity is low, corresponding to 28% of identity in the 146 amino acids aligned. however, the conservative spacing between the cysteine residues suggest that this similarity might have f ... | 1995 | 7501647 |
| sequence analysis and comparison of ribosomal dna from bovine neospora to similar coccidial parasites. | the nuclear small subunit ribosomal rna (nss-rrna) gene sequence of neospora spp. isolated from cattle was analyzed and compared to the sequences from several closely related cyst-forming coccidial parasites. double-stranded dna sequencing of 5 bovine neospora spp. isolates (bpa1-4), 2 neospora caninum isolates (nc-1 and nc-3), and 3 toxoplasma gondii isolates (rh, gt-1, ct-1) were performed and compared to each other, as well as to other sequences available in genbank for the nc-1 isolate, sarc ... | 1995 | 7623193 |
| parasitic apicomplexans harbor a chlorophyll a-d1 complex, the potential target for therapeutic triazines. | ultrastructural evidence is presented for the presence of plastid-like organelles in toxoplasma gondii, sarcocystis muris, babesia ovis, and plasmodium falciparum. in addition, it was shown that merozoites of t. gondii contain protochlorophyllidae a and traces of chlorophyll a bound to the photosynthetic reaction centers i ps i and ps ii. a psba gene was isolated from merozoites of s. muris by the polymerase chain reaction (pcr). partial sequencing of the pcr product revealed that the herbicide- ... | 1995 | 7770426 |
| [the role of the endoplasmic reticulum in the formation of specialized cellular organelles in parasitic sarcosporidia]. | a cytological study was made of participation of the endoplasmic reticulum in the formation of specific cell organelles in sarcocystis muris zoites. the data presented in this paper confirm our earlier assumption (radchenko, 1987) that the golgi adjunct is a structure made of four membranes of the smooth endoplasmic reticulum (er) channels. this organelle appeared to be available in all the three cell types recognized within the sarcocyst: metrocytes, intermediate cells and merozoites. in the di ... | 1994 | 7809974 |
| cdna clones of sarcocystis muris (apicomplexa) encoding a repetitive, arginine-rich region of a putative microneme antigen. | | 1994 | 8073025 |
| characterization of cdna clones encoding a major microneme antigen of sarcocystis muris (apicomplexa) cyst merozoites. | two monoclonal antibodies directed against a microneme antigen of sarcocystis muris cyst merozoites (16/17 kda band doublet) were used to isolate cdna clones from a lambda zap expression library. restriction analysis revealed that the inserts were highly similar, with sizes ranging between 1.8 and 2.3 kb. in addition, a full-length cdna insert of 2.6 kb was obtained by hybridization screening. on northern blots, a single mrna species of 2.7 kb was detected by a cdna-derived probe. southern blot ... | 1993 | 8114823 |
| identification of opossums (didelphis virginiana) as the putative definitive host of sarcocystis neurona. | sarcocystis neurona is an apicomplexan that causes equine protozoal myeloencephalitis (epm) in north and south america. horses appear to be an aberrant host, because the merozoites continually divide in the central nervous system, without encysting. the natural host species has not previously been identified. the small subunit ribosomal rna (ssurna) gene of s. neurona was compared to those of sarcocystis muris, sarcocystis cruzi, toxoplasma gondii, and cryptosporidium parvum to identify a unique ... | 1995 | 8544064 |
| [an electron microscopic study of the natural death of cyst cells in sarcosporidia. i. ultrastructural changes in the cytoplasm of the cyst cells of sarcocystis muris and s. bovifelis]. | a complicated development of sarcocystis muris and s.bovifelis, within tissue cysts (sarcocysts), was examined in terms of a phenomenon of programmed cell death well-known in metazoa. it looks likely that this phenomenon of versatile significance in living organisms has not yet been followed in parasitic protozoa. this is the first attempt to find out a general picture of morphological changes, occurring in the course of natural death in parasitic protozoa. with electron microscope, a study was ... | 1995 | 8658980 |
| [an electron microscopic study of the natural death of cyst cells in sarcosporidia. ii. ultrastructural changes in the nuclei of cyst cells of sarcocystis muris]. | nuclear changes in cyst cells, developing within 6 and 10 month old sarcocysts of sarcocystis muris, were followed in terms of the programmed cell death phenomenon. this communication extends our previous studies in the cytoplasm of s. muris cyst cells (radchenko et al., 1995) to include particular nuclear changes in different involve changes in nuclear configuration: the original spherical from is progressively substituted for irregular or lobulated shapes. this may suggest some corresponding c ... | 1995 | 8658981 |
| in vitro biosynthesis and in vivo processing of the major microneme antigen of sarcocystis muris cyst merozoites. | the cdna clone psm/1.6 encoding the 26.5-kda precursor molecule of the 16/17-kda microneme antigen of sarcocystis muris cyst merozoites was expressed in a cell-free translation/translocation system to study translocation of the protein across membranes. the antigen was found to be translocated across heterologous endoplasmic reticulum membranes. translocation was accompanied by cleavage of a signal peptide to create a 23-kda polypeptide that was completely protected from digestion with proteinas ... | 1996 | 8738288 |
| characterization of genomic clones encoding two microneme antigens of sarcocystis muris (apicomplexa). | subgenomic libraries were constructed from sarcocystis muris total dna. hybridization screening with a microneme-specific cdna probe resulted in two clones that were sequenced. the amino acid sequences deduced showed 87% homology among each other. three different domains were recognized within both polypeptides. domain i includes the putative n-terminal signal sequence. domain ii represents a strongly hydrophilic region, entirely homologous in the two genes. domain iii encodes the mature polypep ... | 1996 | 8801555 |
| development of a polymerase chain reaction assay for the diagnosis of neosporosis using the neospora caninum 14-3-3 gene. | neospora caninum is a recently described apicomplexan parasite which causes neuromuscular disease in dogs, and abortion and neonatal morbidity in cattle, sheep and horses. morphological similarities and serological cross-reactivity between n. caninum and the closely related parasite toxoplasma gondii, have resulted in the frequent misdiagnosis of neosporosis as toxoplasmosis. this report describes the isolation and characterization of an n. caninum cdna clone encoding a 14-3-3 protein homologue. ... | 1996 | 8992315 |
| [the effect of coccidia of the genus sarcocystis on the morphofunctional organization of the skeletal musculature in experimentally infected mice. i. the muscle fiber]. | at the ultrastructural level, the cellular response of skeletal muscles on developing sarcocystis muris sarcocysts has been followed in mice at different times after sporocyst feeding, i.e. in 1, 2.5, 6 and 10 months, resp. the developing cyst creates a progressive degeneration of the infected muscle cell that involves organelle disorganization and formation of numerous vacuoles in the cytoplasm as a consequence of cell edema. products of the host cell degradation, shaped as fibrillar-granular s ... | 1996 | 9027009 |
| [the effect of coccidia of the genus sarcocystis on the morphofunctional organization of the skeletal musculature in experimentally infected mice. ii. the connective tissue elements of the endomysium]. | as an extension of the previous communication (radchenko, et al., 1996), a study was made of the response of connective tissue elements, surrounding the sarcocystis muris infected muscle fibers. as earlier, the s. muris sarcocysts were examined in mice 1, 2.5, 6, and 10 months after sporocyst feeding. within the first 2.5 months after infection, marked accumulations of lymphocyte-like cells and collagen fibres are observed in the endomysium, and simultaneously the activity of capillary endotheli ... | 1996 | 9027010 |
| [one of the possible ways of propagation of the unicellular parasites sarcocystis muris in the musculature of the intermediate host]. | 20 laboratory mice (mus musculus) were fed each a single dose of 20,000 sarcocystis muris sporocysts to be then sacrificed 1, 2.5, 4, 6 and 10 months following infection (p.i.). a visual infection of the murine corps demonstrated that the number of sarcocysts per animal increased regularly as the time of infection was progressing, being eventually higher after 6 and 10 months p.i. than within 1-4 months p.i. this phenomenon was poorly understood from the knowledge that the tissue cysts (sarcocys ... | 1998 | 9541976 |
| expression, purification, and biochemical characterization of a recombinant lectin of sarcocystis muris (apicomplexa) cyst merozoites. | the mature major microneme protein of sarcocystis muris cyst merozoites, which is known as a dimeric lectin with high affinity to galactose and some of its derivatives, was expressed in escherichia colias a histidine-tagged fusion protein. the recombinant polypeptide, which was recognized by a monoclonal antibody directed against the native lectin, was purified from inclusion bodies after solubilization and refolding, using a combination of metal chelate and lactose affinity chromatography. the ... | 1998 | 9557874 |
| amplification of genomic dna fragments of sarcocystis muris (apicomplexa) cyst merozoites encoding a thiol (cysteine) proteinase. | parasites of the phylum apicomplexa (sporozoa) cause diseases such as malaria, toxoplasmosis, or intestinal coccidiosis. invasive stages possess typical apical organelles such as dense granules that harbor a broad range of polypeptides that are believed to take part in the parasite-host cell interaction. in previous studies a 26-kda polypeptide of dense granules from sarcocystis muris cyst merozoites (bradyzoites) was characterized as a thiol (cysteine) proteinase. in this paper a method is demo ... | 1998 | 9694376 |
| isolation and characterization of cdna clones encoding a 32-kda dense-granule antigen of sarcocystis muris (apicomplexa). | a monoclonal antibody (2f4) directed against a 32-kda dense-granule antigen of sarcocystis muris cyst merozoites (bradyzoites) was used to screen a lambda zap cdna expression library. a clone with an insert of 1.4 kb in length (dg 32/1) was isolated. a fusion protein derived from bacteria harbouring the recombinant plasmid dg 32/1 reacted with monoclonal antibody (mab) 2f4. southern blot hybridization suggests that the gene is present as a single copy. on northern blots, a single mrna species of ... | 1998 | 9694377 |
| the severe combined immunodeficient mouse as a definitive host for sarcocystis muris. | peroral and intraperitoneal inoculation of severe combined immunodeficient (scid) mice with cystozoites of three coccidia of the genus sarcocystis (protozoa, apicomplexa; s. dispersa, sarcocystis sp., and s. muris) revealed that after peroral administration, only s. muris could develop in the immunodeficient mouse host. the cystozoites of s. muris transformed into gamonts and, after fertilization, performed sporulation with the production of infectious sporocysts in the small intestine of the sc ... | 1999 | 10431742 |
| isolation and characterization of a cdna clone encoding a thiol proteinase of sarcocystis muris cyst mero' zoites (apicomplexa). | a cdna library of sarcocystis muris cyst merozoites was screened using a digoxigenin-labeled probe. this probe was derived from a 504-bp polymerase-chain-reaction fragment representing part of a thiol proteinase. several cdna clones were isolated, one of which (ph08) consists of a nucleotide sequence of 1694 bp and encodes the complete prepropolypeptide of a cathepsin l-like proteinase. ph08 contains an open reading frame of 394 amino acid (aa) residues with a 46-residue signal sequence, which i ... | 1999 | 10431744 |
| characterization of a genomic region encoding the 32-kda dense granule antigen of sarcocystis muris (apicomplexa). | two subgenomic libraries constructed from sarcocystis muris total dna were screened with a cdna probe, specific for a 32-kda protein associated with the dense granules. two clones reacted positively and were isolated, gdg 32/1 and gdg 32/2. genomic clone gdg32/1 and part of clone gdg 32/2 have been sequenced. the composite nucleotide sequence of these genomic clones comprises 4.34 kb. it contains a 5' region of 2.14 kb, a first coding region (222 bp, exon i), a noncoding region (608 bp, intron), ... | 1999 | 10540954 |
| a microneme protein from eimeria tenella with homology to the apple domains of coagulation factor xi and plasma pre-kallikrein. | microneme organelles are present in all apicomplexan protozoa and contain proteins that are critical for parasite motility and host cell invasion. one apicomplexan-wide family of microneme proteins has been identified with members that are characterised by the possession of thrombospondin type i repeats, conserved adhesive motifs which are implicated in binding to glycosaminoglycan chains. in this paper we describe a micronemal glycoprotein, etmic 5, from eimeria tenella which contains eleven cy ... | 2000 | 10717305 |
| sarcocystis muris--a rare case in laboratory mice. | in a lifespan transgeneration study under standard laboratory conditions using a total of 4682 cba/j mice, unusual intramuscular inclusions were found in the diaphragm, heart and skeletal muscle of one mouse using light microscopy. located within the myocytes, they caused no visible tissue reaction. cross-sections of these spherical and cystic lesions showed numerous banana-shaped structures, identified as permanent parasitic bradyzoites, which permitted these infections to be diagnosed microsco ... | 1999 | 10778789 |
| sarcocystis muris possesses both diheteroxenous and dihomoxenous characters of life cycle. | the cystozoites of sarcocystis muris were infective to other mice after peroral inoculation. they transformed into gamonts and after fertilization underwent sporulation with the production of infectious oocysts/sporocysts in the lamina propria of the small intestine. the present study demonstrated that s. muris possesses both diheteroxenous and dihomoxenous life cycle and can be transmitted by the cannibalism among mice. | 2000 | 10958479 |
| the toxoplasma micronemal protein mic4 is an adhesin composed of six conserved apple domains. | the initial stage of invasion by apicomplexan parasites involves the exocytosis of the micronemes-containing molecules that contribute to host cell attachment and penetration. mic4 was previously described as a protein secreted by toxoplasma gondii tachyzoites upon stimulation of micronemes exocytosis. we have microsequenced the mature protein, purified after discharge from micronemes and cloned the corresponding gene. the deduced amino acid sequence of mic4 predicts a 61-kda protein that contai ... | 2001 | 11053441 |
| effects of sequence alignment and structural domains of ribosomal dna on phylogeny reconstruction for the protozoan family sarcocystidae. | finding correct species relationships using phylogeny reconstruction based on molecular data is dependent on several empirical and technical factors. these include the choice of dna sequence from which phylogeny is to be inferred, the establishment of character homology within a sequence alignment, and the phylogeny algorithm used. nevertheless, sequencing and phylogeny tools provide a way of testing certain hypotheses regarding the relationship among the organisms for which phenotypic character ... | 2000 | 11110900 |
| susceptibility of natural hybrids between house mouse subspecies to sarcocystis muris. | a previous study showed that the susceptibility of hybrids between two mus musculus musculus and mus musculus domesticus did not apply to every parasite. the authors proposed that only parasites which exerted enough constraints would induce the selection of poorly compatible systems of resistance in the subspecies. this study completes the previous work. experimental infections of mice of the two subspecies and their hybrids with the tissue-dwelling protozoan sarcocystis muris show that hybrids ... | 2001 | 11165266 |
| multiple origin of the dihomoxenous life cycle in sarcosporidia. | although their ssrrna gene sequences are closely related, the lizard sarcosporidia (apicomplexa, sarcocystidae) sarcocystis lacertae and sarcocystis gallotiae posses heteroxenous and dihomoxenous life cycles, respectively. when aligned with available sarcosporidian ssrrna genes, both species constitute a monophyletic clade that is only distantly related with sarcosporidia that have a viperid snake as their definitive host (sarcocystis sp., sarcocystis atheridis). to test the phyletic status of t ... | 2001 | 11306120 |
| myosin diversity in apicomplexa. | a polymerase chain reaction (pcr) screen was used to examine the diversity of myosins in 7 apicomplexan parasites: toxoplasma gondii, plasmodium falciparum, neospora caninum, eimeria tenella, sarcocystis muris, babesia bovis, and cryptosporidium parvum. using degenerate pcr primers compatible with the majority of known myosin classes, putative myosin sequences were obtained from all of these species. all of the sequences obtained showed greatest similarity to previously identified apicomplexan m ... | 2001 | 11318578 |
| [the relationship between cytosolic ca2+ and two kinds of granule number in activated platelets]. | the numerical density (nv) of the alpha-granule (alpha g) and dense granule (dg) measured by using electron microscopic morphometry and the cytosolic free calcium concentration ([ca2+]i) by using ca-fluorescent indicator were observed for studying the relationship of [ca2+]i and nv of alpha g and dg in platelets activated by a23187, thrombin or adp. the results showed that the [ca2+]i rose markedly by a23187. thrombin or adp could also induce an increase of [ca2+]i concentration-dependently. und ... | 1998 | 11324533 |
| characterization and crystallization of a novel sarcocystis muris lectin, sml-2. | a novel lectin (sml-2) consisting of 138 amino acids was isolated from cyst merozoites of sarcocystis muris and sequenced by edman degradation and mass spectrometry. all 12 cysteinyl residues are involved in disulfide bridges, four of which are attributed to a characteristic pattern of cysteines as found in the so-called pan-module superfamily. crystals of sml-2 diffracting to 2.1 a resolution at a synchrotron were grown by the hanging-drop vapour-diffusion technique. they belong to the space gr ... | 2001 | 11418777 |
| [effect of developing sarcocystis muris tissue cysts on ultrastructural change of murine muscle fibers]. | a study was made of the influence exerted by developing sarcocysts of sarcocystis muris on the ultrastructural organization of muscle fibres, both harbouring the sarcocysts (hsm) and sarcocyst-free (sfm), from skeletal muscles of experimentally infected mice. muscle fibres of non-infected mice of the same age served as a control. mice were sacrificed 6 months following feeding s. muris oocysts (or sporocysts). the developing sarcocysts seriously destroyed hsm: their myofilaments were no hold in ... | 2001 | 11552655 |
| effects of specific monoclonal antibodies to dense granular proteins on the invasion of toxoplasma gondii in vitro and in vivo. | although some reports have been published on the protective effect of antibodies to toxoplasma gondii surface membrane proteins, few address the inhibitory activity of antibodies to dense granular proteins (gra proteins). therefore, we performed a series of experiments to evaluate the inhibitory effects of monoclonal antibodies (mabs) to gra proteins (gra2, 28 kda; gra6, 32 kda) and surface membrane protein (sag1, 30 kda) on the invasion of t. gondii tachyzoites. passive immunization of mice wit ... | 2001 | 11590913 |
| molecular characterisation of a major 29 kda surface antigen of sarcocystis neurona. | a gene encoding a major 29 kda surface antigen from sarcocystis neurona, the primary causative agent of equine protozoal myeloencephalitis (epm), was cloned, sequenced, and expressed as a recombinant protein. a cdna library was prepared in the expression vector lambda zap from polya+mrna isolated from s. neurona merozoites cultivated in vitro. random sequencing of 96 clones identified a clone of an abundant transcript having a translated amino acid sequence with 30% identity to the 31-kda surfac ... | 2002 | 11812499 |
| notes on coccidian phylogeny, based on the apicoplast small subunit ribosomal dna. | we performed a phylogenetic analysis, based on the partial small subunit rrna gene (ssu rrna) sequences from 13 apicoplasts (including new sequences of sarcocystis muris and hyaloklossia lieberkuehni) and 16 other plastids, with cyanobacteria as an outgroup. the apicoplast sequences formed a highly supported monophyletic clade with two distinct clades, representing coccidia and haemosporidia, with coccidia divided into eimeriidae and sarcocystidae subclades. we confirmed the phylogenetic positio ... | 2002 | 11999025 |
| [the structure of the surface apparatus in sarcocysts of sarcosporidia in the persistence process]. | the structure of the sarcocyst surface apparatus (ssa) was investigated for two sarcosporidian species: sarcocystis muris (non-pathogenic) and s. fusiformis (pathogenic). the surface membrane, being the main ssa subsystem, makes numerous vesicle-like protrusions with different ultrastructural patterns. this made it possible to distinguish between four and three types of these protrusions in s. fusiformis and s. muris, respectively. vesicles of similar structure, pinched off from the fully formed ... | 2002 | 12236105 |
| cloning, sequencing and recombinant expression of the open reading frame encoding a novel member of the sarcocystis muris (apicomplexa) microneme lectin family. | micronemes are characteristic secretory organelles located within the apical cell region of apicomplexan parasites. the protein contents are exocytosed during an early phase of host cell invasion and contribute to parasite motility and the invasion of target cells. we report here on the cloning and heterologous expression of a novel member of the sarcocystis muris microneme lectin family. the deduced amino acid sequence is in total agreement with that obtained after sequencing the native protein ... | 2003 | 12743810 |
| [the role of the sarcocyst surface apparatus in utilizing the host cell muscle structures]. | the participation of the sarcocyst surface apparatus (ssa) of two sarcosporidian species, sarcocystis muris and s. ovifelis (coccidia, sporozoa, apicomplexa), in degradation of disrupted host cell substances was investigated. after degradation, these substances are transported through the membrane of the ssa to the sarcocyst ground substance (gs), but this process cannot be regarded as endocytosis. at first, the transported substances were found in ssa pits in the form of fibrillar structures. l ... | 2003 | 14521051 |
| [incidence of trypanosoma lewisi, sarcocystis muris, species of spirochaeta, and larvae of microfilaria in the rats of panama city and its suburbs]. | | 1951 | 14858447 |
| low-resolution ab initio phasing of sarcocystis muris lectin sml-2. | structural analysis of the lectin sml-2 faced difficulties when applying standard crystallographic phasing methods. the connectivity-based ab initio phasing method allowed the computation of a 16 a resolution fourier synthesis and the derivation of primary structural information. it was found that sml-2 crystals have three dimers in the asymmetric part of the unit cell linked by a noncrystallographic symmetry close to translation by (0, 0, 1/3). a clear identification of the noncrystallographic ... | 2006 | 16627947 |
| [the structural and functional analysis of the surface apparatus in four species of sarcocystis (sporozoa, apicomplexa)]. | the comparable ultrastructural analysis of the sarcocyst surface apparatus (ssa) was made for four species of sarcocystis: sarcocystis muris, s. fusiformis, s. medusiformis, and sarcocystis sp. from buffalo heart muscles. in all these species, ssa contains a surface membrane, overmembrane complex with glycocalyx, and submembrane complex made of two glycoprotein ssa primembrane layers. ssa makes numerous primary vesicle-like protrusions and pits in between. some vesicles containing two layers, pm ... | 2005 | 16706206 |
| experimental sarcosporidiosis in the guinea-pig and its relation to a case of sarcosporidiosis in man. | 1. guinea-pigs, naturally uninfected by sarcosporidia, were infected by feeding them with rat's muscle that was naturally infected by sarcocystis muris, and by ripe mobile sporozoites from the same source. the infection was not visible grossly, but was detected upon very careful search through many sections of muscle. 2. sarcosporidia were not found in the guinea-pigs until after an interval of 164 days from the first feeding, or 152 days after the most favorable feeding, when many teased-out mo ... | 1910 | 19867309 |
| further observations on the transmission of sarcocystis muris by feeding. | | 1905 | 19971680 |
| pan-modular structure of microneme protein sml-2 from the parasite sarcocystis muris at 1.95 å resolution and its complex with 1-thio-β-d-galactose. | the microneme protein sml-2 is a member of a small family of galactose-specific lectins that play a role during host-cell invasion by the apicomplexan parasite sarcocystis muris. the structures of apo sml-2 and the 1-thio-β-d-galactose-sml-2 complex were determined at 1.95 and 2.1 å resolution, respectively, by sulfur-sad phasing. highly elongated dimers are formed by pan-domain tandems in the protomer, bearing the galactose-binding cavities at the distal apple-like domains. the detailed structu ... | 2011 | 22101820 |
| tggra23, a novel toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network. | toxoplasma gondii is an intracellular protozoan parasite, which relies on a specialized compartment, the parasitophorous vacuole (pv), to survive within host cells. dense granules within the parasite release a large variety of proteins to maintain the integrity of the vacuole structure. here, we identified a novel dense granule protein in t. gondii, tggra23, which is a homolog of the sarcocystis muris dense granule protein, smdg32. recombinant tggra23 (rtggra23) expressed in escherichia coli as ... | 2013 | 23583316 |
| experimental transmission of sarcocystis muris (apicomplexa: sarcocystidae) sporocysts from a naturally infected cat (felis catus) to immunocompetent and immunocompromised mice. | cats serve as definitive hosts for zoonotic toxoplasma gondii , a protozoan that threatens human reproductive health, but they also excrete sporocysts of related protozoan that pose no known human health risk. here we provide the first definitive evidence for natural infection with the enzootic parasite sarcocystis muris, one such enzootic parasite. sporulated sarcocystis sp. sporocysts were found in rectal contents of an adult feral cat ( felis catus ) in giza, egypt. after these sporocysts wer ... | 2013 | 23758571 |