| the nucleotide sequence of the entire ribosomal dna operon and the structure of the large subunit rrna of giardia muris. | the total nucleotide sequence of the rdna of giardia muris, an intestinal protozoan parasite of rodents, has been determined. the repeat unit is 7668 basepairs (bp) in size and consists of a spacer of 3314 bp, a small-subunit rrna (ssu-rrna) gene of 1429, and a large-subunit rrna (lsu-rrna) gene of 2698 bp. the spacer contains long direct repeats and is heterogeneous in size. the lsu-rrna of g. muris was compared to that of the human intestinal parasite giardia duodenalis, to the bird parasite g ... | 1992 | 1404417 |
| cloning and restriction enzyme mapping of ribosomal dna of giardia duodenalis, giardia ardeae and giardia muris. | in an attempt to study giardia at the dna sequence level, the rrna genes of three species, giardia duodenalis, giardia ardeae and giardia muris were cloned and restriction enzyme maps were constructed. the rdna repeats of these giardia show completely different restriction enzyme recognition patterns. the size of the rdna repeat ranges from approximately 5.6 kb in g. duodenalis to 7.6 kb in both g. muris and g. ardeae. these size differences are mainly attributable to the variation in length of ... | 1991 | 1922199 |
| nucleotide sequence of the 5.8s and large subunit rrna genes and the internal transcribed spacer and part of the external spacer from giardia ardeae. | | 1991 | 1945890 |
| axenic culture and characterization of giardia ardeae from the great blue heron (ardea herodias). | trophozoites of giardia ardeae were obtained from the great blue heron (ardea herodias) and established in axenic culture using the tyi-s-33 medium. the generation time in culture for g. ardeae was 22-25 hr, which was 3-fold longer than for giardia duodenalis (wb strain). a morphological comparison of trophozoites in the original intestinal isolate to those grown in culture revealed that they were identical for the following characteristics: a pyriform-shaped body, a ventral adhesive disc with a ... | 1990 | 2213415 |
| unique phylogenetic position of diplomonadida based on the complete small subunit ribosomal rna sequence of giardia ardeae, g. muris, g. duodenalis and hexamita sp. | complete small-subunit rrna (ssu-rrna) coding region sequences were determined for two species of the intestinal parasite giardia: g. ardeae and g. muris, both belonging to the order diplomonadida, and a free-living member of this order, hexamita sp. these sequences were compared to published ssu-rdna sequences from a third member of the genus giardia, g. duodenalis (often called g. intestinalis or g. lamblia) and various representative organisms from other taxa. of the three giardia sequences a ... | 1993 | 8422968 |
| morphological and molecular characterization of giardia isolated from the straw-necked ibis (threskiornis spinicollis) in western australia. | following the first report of avian giardia infection in australia, isolates of the parasite recovered from naturally infected straw-necked ibis (theskiornis spinicollis) were characterized using median body morphology, scanning electron microscopy, multilocus enzyme electrophoresis, random amplified polymorphic dna (rapd), and small subunit ribosomal rna (ssu-rrna) analyses. results were compared with giardia from other birds and mammals, and the extent of genetic diversity between a range of i ... | 1996 | 8885877 |
| genotyping of giardia lamblia isolates from humans in china and korea using ribosomal dna sequences. | genetic characterization of a total of 15 giardia lamblia isolates, 8 from anhui province, china (all from purified cysts) and 7 from seoul, korea (2 from axenic cultures and 5 from purified cysts), was performed by polymerase chain reaction amplification and sequencing of a 295-bp region near the 5' end of the small subunit ribosomal dna (eukaryotic 16s rdna). phylogenetic analyses were subsequently conducted using sequence data obtained in this study, as well as sequences published from other ... | 2000 | 10958483 |
| giardiasis in a white stork in the netherlands. | giardia sp. was found in the white stork (ciconia ciconia) in the netherlands for the first time. the giardia sp. trophozoites that were found in the feces of a 6-wk-old white stork, were examined by light microscopy. the parasites closely resembled giardia ardeae that had been isolated by others from several species of wading birds belonging to the order ciconiiformes, sharing a deeply notched adhesive disk, a single caudal flagellum, and a single round median body. serologically, the parasites ... | 2000 | 11085441 |
| improved specificity for giardia lamblia cyst quantification in wastewater by development of a real-time pcr method. | the protozoan parasite giardia lamblia is the most common cause of waterborne disease outbreaks associated with drinking water in the united states. the conventional method used for the enumeration of giardia cysts in water is based on immunofluorescence with monoclonal antibodies. it is tedious and time-consuming and has the major drawback to be non-specific for the only species infecting humans, g. lamblia. we have developed a real-time polymerase chain reaction (pcr) method using fluorescent ... | 2004 | 15003687 |
| development of species-specific rdna probes for giardia by multiple fluorescent in situ hybridization combined with immunocytochemical identification of cyst wall antigens. | in this study, we describe the development of fluorescent oligonucleotide probes to variable regions in the small subunit of 16s rrna in three distinct giardia species. sense and antisense probes (17-22 mer) to variable regions 1, 3, and 8 were labeled with digoxygenin or selected fluorochomes (fluorx, cy3, or cy5). optimal results were obtained with fluorochome-labeled oligonucleotides for detection of rrna in giardia cysts. specificity of fluorescent in situ hybridization (fish) was shown usin ... | 2005 | 15879572 |
| molecular characterization of giardia psittaci by multilocus sequence analysis. | multilocus sequence analyses targeting small subunit ribosomal dna (ssu rdna), elongation factor 1 alpha (ef1α), glutamate dehydrogenase (gdh), and beta giardin (β-giardin) were performed on giardia psittaci isolates from three budgerigars (melopsittacus undulates) and four barred parakeets (bolborhynchus lineola) kept in individual households or imported from overseas. nucleotide differences and phylogenetic analyses at four loci indicate the distinction of g. psittaci from the other known giar ... | 2012 | 22921500 |