| life cycle of tritrichomonas muris. | morphological and physiological studies were carried out on a flagellate, tritrichomonas muris, in golden hamsters. the experiments confirmed that a pseudocyst and an intermediate form exist in addition to the trophozoite in the life cycle; furthermore it was shown that the pseudocyst was the infective stage, and that a true cyst did not occur. | 1987 | 2958972 |
| the use of metronidazole, tinidazole and dimetridazole in eliminating trichomonads from laboratory mice. | metronidazole, tinidazole and dimetridazole were administered in the drinking water for 5 days to mice experimentally infected with tritrichomonas muris and tetratrichomonas microta. mice were successfully infected with t. muris and t. microta recovered from infected gerbils. the trichomonas infection was successfully eliminated in mice given a 1% sucrose solution containing 2.5 mg/ml metronidazole or tinidazole. mice receiving 1.0 mg/ml metronidazole, 1.0 mg/ml tinidazole and 1.2, 5.0 and 10.0 ... | 1988 | 3230872 |
| [the presence of cysts in tritrichomonas muris]. | cysts of tritrichomonas muris are reported. the morphology of this evolution stage is described under light and electron microscopy. the biologic and epidemiologic importance of this finding is discussed. | 1987 | 3444923 |
| fine structure of the mastigont system in tritrichomonas muris (grassi). | | 1971 | 4332235 |
| a new method for the serial cultivation of tritrichomonas muris. | | 1983 | 6632453 |
| minimal infectious doses and prepatent periods in giardia muris, spironucleus muris and tritrichomonas muris. | the minimal infectious doses (mid) of giardia muris cysts, spironucleus muris cysts and tritrichomonas muris pseudocysts for athymic mice were determined. a novel micromanipulator-aided technique was developed for selecting small exact numbers of specified fresh or old cysts/pseudocysts from a faecal homogenate. this technique can also be of value in manipulating protozoan parasites of any particular species. only fresh cysts/pseudocysts, which appeared bright under phase contrast optics, were i ... | 1983 | 6673420 |
| tritrichomonas muris in the hamster: pseudocysts and the infection of newborn. | female golden hamsters, either in the last week of pregnancy or in the first weeks of nursing, excreted in their feces variable numbers of pseudocysts of tritrichomonas muris. pseudocysts examined by electron microscopy had internalization of the 3 anterior flagella and the undulating membrane with its recurrent flagellum. the undulating membrane and the associated marginal lamellae were characteristic of t. muris. pseudocysts gradually become motile after 2 or more hours of incubation in medium ... | 1980 | 7218184 |
| phylogeny of trichomonads based on partial sequences of large subunit rrna and on cladistic analysis of morphological data. | several domains of large subunit rrna from nine trichomonad species have been sequenced. molecular phylogenies obtained with parsimony and distance methods demonstrate the trichomonads are a monophyletic group which branches very early in the eukaryotic tree. the topology of the trees is in general agreement with traditional views on evolutionary and systematic relationships of trichomonads. a clear dichotomy is noted between the subfamily trichomonadinae and the subfamily tritrichomonadinae. in ... | 1993 | 8330027 |
| identification of pseudocysts of tritrichomonas muris in armenian hamsters and their transmission to mice. | | 1999 | 10403448 |
| microwave processing for scanning electron microscopy. | the normal processing of biological samples for scanning electron microscopy, includes treatment with aldehyde (1 to 2 hours), postfixation with osmium (1 hour), followed by dehydration in a ascending grade of ethanol (30 a 100%), 10 to 15 minutes in each step, and finally drying. this procedure takes at least 8 hours. in this work, samples of mosquitoes (aedes), protozoa (tritrichomonas muris), bacteria (clostridium oceanicum), murine liver, and small intestine were processed in the same manner ... | 2000 | 10694907 |
| post-translational glutamylation and tyrosination in tubulin of tritrichomonads and the diplomonad giardia intestinalis. | glutamylated and tyrosinated tubulin were localized in giardia intestinalis and selected trichomonads of the tritrichomonadinae subfamily, using specific monoclonal antibodies directed at each of the post-translational modifications. analysis was carried out using indirect immunofluorescence microscopy. although trichomonad tubulins remained unlabeled by anti-tyrosine tubulin (tub-1a2), the presence of the glutamylation motif (gt 335) was confirmed and found to differ in distribution among tritr ... | 2002 | 11822738 |
| microbiological contamination of laboratory mice and rats in korea from 1999 to 2003. | to survey the microbiological contamination of laboratory mice and rats in korea during a 5-year period, we monitored animals housed in mouse and rat facilities with either barrier or conventional systems. at barrier and conventional mouse facilities, the most important pathogen identified was mouse hepatitis virus (mhv), while mycoplasma pulmonis was the most important pathogen at conventional rat facilities. interestingly, hantavirus was recovered from both barrier and conventional mouse facil ... | 2006 | 16508207 |
| effects of tritrichomonas muris on the mouse intestine: a proteomic analysis. | although tritrichomonas muris is a common parasite often detected in experimental animals including mice, its pathogenesis in host animals remains unclear. proteomics can be used to specifically analyze biochemical host-parasite interaction and immune responses of the host to parasites. however, proteomics have not yet been applied to t. muris studies. here, the effects of t. muris on the host were analyzed by proteomics. we found that 10 different proteins were expressed in t. muris-infected mi ... | 2009 | 19897938 |
| fluorescence in situ hybridization for identification of tritrichomonas foetus in formalin-fixed and paraffin-embedded histological specimens of intestinal trichomoniasis. | in the present study a highly species-specific oligonucleotide sequence of tritrichomonas foetus 18s rrna was used to design an antisense probe for identification of t. foetus in formalin-fixed and paraffin-embedded histological specimens by means of fluorescence in situ hybridization (fish). using archival histological specimens from several species with light microscopic evidence of intestinal trichomoniasis, and under optimized hybridization conditions, the probe positively identified trichom ... | 2010 | 20447769 |
| cytoskeleton in trichomonads: ii. immunological and biochemical characterization of the preaxostylar fibres and undulating membrane in the genus tritrichomonas. | the production of 3 monoclonal antibodies (mabs) and the use of immunocytochemical techniques such as immunofluorescence (if), immunoblotting (ib) and immunogold staining (igs) reveal that the preaxostylar fibres of tritrichomonas foetus are composed of at least 3 polypeptides of 55, 53 and 46 kda. two of these mabs cross-react with tritrichomonas mobilensis and one with tritrichomonas augusta and tritrichomonas muris on polypeptides with very similar molecular weights (m.w.) however, no cross-r ... | 2011 | 23195736 |
| microbiological survey of mice (mus musculus) purchased from commercial pet shops in kanagawa and tokyo, japan. | information regarding the prevalence of infectious agents in mice in pet shops in japan is scarce. this information is particularly useful for minimizing the risk of potential transmission of infections to laboratory mice. therefore, we surveyed infectious agents in mice from pet shops in kanagawa and tokyo, japan. the survey was conducted in 28 mice from 5 pet shops to screen for 47 items (17 viruses, 22 bacteria and fungi, 10 parasites) using culture tests, serology, pcr, and microscopy. the m ... | 2014 | 25502736 |
| influence of rack design and disease prevalence on detection of rodent pathogens in exhaust debris samples from individually ventilated caging systems. | sampling of bedding debris within the exhaust systems of ventilated racks may be a mechanism for detecting murine pathogens in colony animals. this study examined the effectiveness of detecting pathogens by pcr analysis of exhaust debris samples collected from ventilated racks of 2 different rack designs, one with unfiltered air flow from within the cage to the air-exhaust pathway, and the other had a filter between the cage and the air-exhaust pathway. for 12 wk, racks were populated with eithe ... | 2016 | 27931317 |
| determination of internal transcribed spacer regions (its) in trichomonas vaginalis isolates and differentiation among trichomonas species. | the nucleotide sequence of the 5.8s rrna gene and the flanked internal transcribed spacer (its) regions of six trichomonas vaginalis isolates with different metronidazole sensitivity and geographic origin were genotyped. a multiple sequence alignment was performed with different sequences of other isolates available at the genbank/embl/ddbj databases, which revealed 5 different sequence patterns. although a stable mutation in position 66 of the its1 (c66t) was observed in 26% (9/34) of the t. va ... | 2014 | 24412628 |
| fingerprinting and genetic relationship of trichomonas vaginalis, trichomonas muris and trichomonas gallinae. | because of the common pathology, protein expression and drug resistance mechanisms-though in different hosts- of the luminal trichomonads trichomonas vaginalis, t. gallinae and t. muris, doubts were raised concerning their phylogenetic relationship. the random amplified polymorohic (rapd) technique for genetic dna polymorphism studying was used to genetically characterize and assess their interspecies polymorphism. results showed a high statistical support for the close relationship between the ... | 2013 | 24640874 |
| the common mouse protozoa tritrichomonas muris alters mucosal t cell homeostasis and colitis susceptibility. | the mammalian gastrointestinal tract hosts a diverse community of microbes including bacteria, fungi, protozoa, helminths, and viruses. through coevolution, mammals and these microbes have developed a symbiosis that is sustained through the host's continuous sensing of microbial factors and the generation of a tolerant or pro-inflammatory response. while analyzing t cell-driven colitis in nonlittermate mouse strains, we serendipitously identified that a nongenetic transmissible factor dramatical ... | 2016 | 27836928 |
| use of ronidazole and limited culling to eliminate tritrichomonas muris from laboratory mice. | tritrichomonas muris is occasionally identified during routine fecal screening of laboratory mice. frequently, entire racks are affected, and because no effective treatment is available, culling of affected mice and rederivation by embryo transfer have been suggested. the current study evaluated whether treatment with ronidazole, a nitroimidazole efficacious against t. fetus infections in cats, combined with limited culling was effective against t. muris in laboratory mice (mus musculus). a subs ... | 2016 | 27423157 |