| [ultrastructure and life cycle of herpetomonas muscarum, "herpetomonas mirabilis" and crithidia luciliae in chrysomyia chloropyga]. | | 1974 | 4155243 |
| phlebotomus papatasi and leishmania major parasites express alpha-amylase and alpha-glucosidase. | alpha-amylase and alpha-glucosidase activities were found in homogenates of young, unfed male and female phlebotomus papatasi and in gut and salivary gland preparations. a significant increase in both enzyme activities in females and of alpha-amylase in males was recorded for flies that had fed overnight on a plant (capparis spinosa). after plant feeding, alpha-amylase activity was relatively lower in female salivary glands and higher in guts, while in the males the activity in the salivary glan ... | 2001 | 11164750 |
| electrophoretic analysis of endonuclease-generated fragments of k-dna, of esterase isoenzymes, and of surface proteins as aids for species identification of insect trypanosomatids. | in order to verify the applicability of biochemical methods for species identification of trypanosomatidae, 13 species of monoxenic trypanosomatids plus the heteroxenous trypanosoma cruzi were comparatively analyzed by three different biochemical methods. insect trypanosomatids examined were: crithidia acanthocephali, c. fasciculata (three varieties), c. luciliae luciliae, c. luciliae thermophila, c. deanei, c. oncopelti, herpetomonas muscarum muscarum, h. megaseliae, h. samuelpessoai, h. mariad ... | 1982 | 6284925 |
| histological observations on larvae of the eye gnat, hippelates pusio (diptera: chloropidae), infected with the flagellate herpetomonas muscarum. | | 1972 | 5029952 |
| two new subspecies of herpetomonas muscarum (leidy, 1856) kent, 1880. | | 1971 | 4109037 |
| purine phosphoribosyltransferases of leishmania mexicana mexicana and other flagellate protozoa. | amastigotes and cultured promastigotes of leishmania mexicana mexicana and l. m. amazonensis, cultured promastigotes of l. donovani and l. tarentolae, and the culture forms of crithidia fasciculata, herpetomonas muscarum muscarum and h. m. ingenoplastis all possessed four phosphoribosyltransferase (prtase) activities: adenine prtase, hypoxanthine prtase, guanine prtase and xanthine prtase. the enzymes of l. m. mexicana required divalent cations for activity; mn2+ or co2+ produced maximal activit ... | 1985 | 3937660 |
| varying kinetoplast ultrastructure in two subspecies of herpetomonas muscarum (leidy). | | 1973 | 4122736 |
| s-adenosylmethionine and transmethylation reactions in trichomonads. | s-adenosylmethionine (sam) levels in trichomonads, a range of trypanosomatids and mouse liver were measured using hplc techniques. the concentrations were found to be similar in each with the exception of herpetomonas muscarum ingenoplastis, which contained approximately ten-fold more. living trichomonads were found to incorporate exogenous l-methionine into intracellular sam and its methyl carbon was also detected in lipids and nucleic acids, presumably through its involvement in transmethylati ... | 1987 | 3108875 |
| effects of herpetomonas muscarum on development and longevity of the eye gnat, hippelates pusio (diptera: chloropidae). | | 1972 | 5044283 |
| phosphomonoesterases of leishmania mexicana mexicana and other flagellates. | amastigotes and log-phase promastigotes of leishmania mexicana mexicana contained distinct acid phosphatase, 3'-nucleotidase and 5'-nucleotidase activities, distinguishable by their response to ph and inhibitors. both tartrate-sensitive and tartrate-resistant acid phosphatase were present in the two forms, amastigotes possessed less tartrate-resistant acid phosphatase than promastigotes. a tartrate-sensitive acid phosphatase was secreted into the medium in large amounts during the growth in vitr ... | 1987 | 3037369 |
| a novel ecto-phosphatase activity of herpetomonas muscarum muscarum inhibited by platelet-activating factor. | in the present work ecto-phosphatase activity in herpetomonas muscarum muscarum has been characterized using live parasites. this enzyme hydrolyzed p-nitrophenylphosphate at a rate of 4.27 nmol pi/mg of protein.min. a ph curve was generated, in which these intact flagellates showed the highest phosphatase activity at ph 6.5. classical inhibitors for acid phosphatase, such as sodium orthovanadate, sodium tartrate, and ammonium molybdate, were used in the experiments and showed different patterns ... | 1998 | 9875238 |
| changes in cell surface anionogenic groups induced by propranolol in herpetomonas muscarum muscarum. | the effects of propranolol (10(-3) mm) on the surface anionic groups of herpetomonas muscarum muscarum were analysed by cell electrophoresis, by ultrastructural cytochemistry and by identification of sialic acids using paper chromatography. differentiation of h. muscarum muscarum induced by propranolol treatment caused a significant increase in the net negative surface charge. binding of cationized ferritin (cf) and colloidal iron hydroxide particles was observed at the cell surface of both untr ... | 1989 | 2543816 |
| anti-alpha-galactosyl antibodies in chagasic patients. possible biological significance. | 1. antibodies from chagasic patients (ch), bound to a column of immobilized purified glycoprotein 25-kda antigen of trypanosoma cruzi epimastigotes (gp25), were partially lytic (70% lysis at 50 micrograms/ml) to cultured metacyclic trypomastigotes (y strain) in a complement-mediated reaction (alternative pathway). 2. antibodies (ch) eluted with galactose from a melibiose-agarose column reacted in elisa titration plates with gp25, whole cells of metacyclic trypomastigotes (y strain), and less int ... | 1988 | 2471563 |
| domestic and wild mammals infection by trypanosoma evansi in a pristine area of the brazilian pantanal region. | the objective of this study was to estimate the trypanosoma evansi infection rate and epizootical status of wild and domestic animals from the brazilian pantanal region using a standardized polymerase chain reaction (pcr). we used a simple dna extraction method based on chelex resin (biorad, usa) on blood eluted from filter paper confetti. primers directed to repetitive nuclear dna sequences were used in the pcr, and could detect 30 fg of t. evansi dna. the analytical specificity of the assay wa ... | 2005 | 15824901 |
| magnesium-dependent ecto-atp diphosphohydrolase activity in herpetomonas muscarum muscarum. | in the present work we characterized the ecto-atp diphosphohydrolase activity of the trypanosomatid parasite herpetomonas muscarum muscarum. this parasite hydrolyzed atp at a rate of 15.52 nmol pi/mg protein/min and this activity reached a maximum at ph 7.5. classical inhibitors of acid phosphatases, such as sodium orthovanadate (navo3), sodium fluoride (naf), and ammonium molybdate presented no effect on this activity. mgcl2, zncl2, and mncl2 stimulated the atp hydrolysis by h. m. muscarum. the ... | 2003 | 14629005 |
| platelet-activating factor (paf) activates casein kinase 2 in the protozoan parasite herpetomonas muscarum muscarum. | herpetomonas muscarum muscarum is a flagellate parasite of the family trypanosomatidae, whose cell differentiation can be triggered by the lipid mediator, paf. in this study we demonstrate for the first time that paf effect relies on the activation of casein kinase 2 (ck2). the classical antagonist of paf receptor, web 2086, abrogated paf-enhanced ck2 activity. ck2 activation by paf was also inhibited when parasite extracts were assayed in the presence of modulators of pkc, mapk, and both ser/th ... | 2002 | 12054663 |
| platelet-activating factor modulates a secreted phosphatase activity of the trypanosomatid parasite herpetomonas muscarum muscarum. | in the present work we characterized the secreted phosphatase activity of the trypanosomatid parasite herpetomonas muscarum muscarum. this housefly parasite hydrolyzed p-nitrophenylphosphate at a rate of 10.26 nmol pi/mg protein/min. classical inhibitors of acid phosphatases, such as sodium orthovanadate (navo3), sodium fluoride (naf), and ammonium molybdate promoted a decrease in this phosphatase activity. when the parasites were assayed in the presence of sodium tartrate, an inhibitor of leish ... | 2001 | 11683365 |
| biological effects of lithium chloride on herpetomonas muscarum muscarum and blastocrithidia culicis (kinetoplastida: trypanosomatidae). | lithium is widely used in medicine as an antidepressive drug and for myelosuppression attenuation during chemotherapy. in spite of abundant literature, questions on the biological action of lithium ions are far from being answered. we have here examined the effects of lithium (10-200 mm) on culture forms of the trypanosomatid protozoa herpetornonas muscarum muscarum and blastocrithidia culicis. incubation of these parasites with licl inhibited cell growth in a concentration-dependent manner, but ... | 1999 | 10461957 |
| the non-pathogenicity of herpetomonas muscarum to laboratory animals. | | 1947 | 20252033 |
| effect of platelet-activating factor on the process of cellular differentiation of herpetomonas muscarum muscarum. | the effects of platelet-activating factor (paf), at doses ranging from 10(-6) m to 10(-10) m, on cell growth and on cell differentiation of herpetomonas muscarum muscarum were investigated. cell differentiation was evaluated by both light and electron microscopy. at the concentrations used, paf did not interfere with the protozoan growth. however, parasites grown in the presence of paf (10(-6) m) were significantly more differentiated than those grown in the absence of paf, since the first day o ... | 1997 | 9225446 |
| cellulase activity of leishmania major in the sandfly vector and in culture. | the secretion of cellulose-degrading enzymes by leishmania promastigotes in culture and in the sandfly vector was demonstrated. two types of activity of cellulase enzyme-complexes were measured: endoglucanases, which randomly cleave cellulose chains and cellobioydrolases, which remove cellobiose from the nonreducing end of the molecule. the assays demonstrated that enzymes with these activities were secreted into the culture medium by leishmania major, l. donovani, and l. braziliensis. these act ... | 1997 | 9183709 |
| ribosomal and kdna markers distinguish two subgroups of herpetomonas among old species and new trypanosomatids isolated from flies. | we examined files of various families for the presence of trypanosomatids. of 592 insects, 113 (19%) were positive. from these insects, we obtained 42 cultures and selected 14 for further analysis. seven of the cultures had the characteristics of herpetomonas species, they displayed typical opisthomastigotes, lacked arginase, possessed a pvu ii restriction site at 360 bp from the 5' end of the small subunit ribosomal gene, and did not possess a hin diii site at 1,500 bp from the ribosomal alpha- ... | 1997 | 9057697 |
| cross-reactivity between trypanosoma cruzi and insect trypanosomatids as a basis for the diagnosos of chagas' disease. | immunological cross-reactivity between trypanosoma cruzi and insect trypanosomatids was demonstrated by immunofluorescence and confirmed by complement fixation, direct agglutination and cross-absorption experiments. as antigens, the following organisms were surveyed: crithidia deanei, crithidia fasciculata, crithidia luciliae, herpetomonas samuelpessoai, herpetomonas megaseliae, herpetomonas muscarum muscarum, leptomonas seymouri and blastocrithidia culicis. sera from patients with chagas' disea ... | 1981 | 6798889 |
| the effect of propranolol on metabolism and on membrane-associated polysaccharide components of herpetomonas muscarum muscarum. | the effects of propranolol on the metabolism (cell growth, cell differentiation and cell respiration) as well as on membrane-associated polysaccharide components of herpetomonas muscarum muscarum were investigated. at the dose of 10(-2) and 10(-3) mm the drug increased significantly the number of the more differentiated opisthomastigote form of the protozoan, whereas at higher concentrations (10(-1) mm) total inhibition of growth occurred with gross cell lysis. propranolol (10(-3) mm) enhanced t ... | 1983 | 6658189 |
| lineage-specific activities of a multipotent mitochondrion of trypanosomatid flagellates. | trypanosomatids are a very diverse group composed of monoxenous and dixenous parasites belonging to the excavate class kinetoplastea. here we studied the respiration of five monoxenous species (blechomonas ayalai, herpetomonas muscarum, h. samuelpessoai, leptomonas pyrrhocoris and sergeia podlipaevi) introduced into culture, each representing a novel yet globally distributed and/or species-rich clade, and compare them with well-studied flagellates trypanosoma brucei, phytomonas serpens, crithidi ... | 2015 | 25557487 |
| presence of a bent helix in fragments of kinetoplast dna minicircles from several trypanosomatid species. | some restriction fragments of kinetoplast minicircles from several trypanosomatid species (leishmania tarentolae, trypanosoma brucei, t. equiperdum, herpetomonas muscarum, crithidia fasciculata, but not t. cruzi) migrate anomalously on polyacrylamide gels. this behavior is probably due to a natural curvature of the helix. bent helices appear to be a common property of kinetoplast minicircles, and may be important for minicircle function. in the case of t. equiperdum, we present evidence that eac ... | 1984 | 6090900 |
| hypergammaglobulinaemia in leishmania donovani infected hamsters: possible association with a polyclonal activator of b cells and with suppression of t cell function. | studies were carried out on the mechanisms by which b lymphocytes are polyclonally activated to secrete antibodies during visceral leishmaniasis. crude extracts of leishmania donovani, the aetiological agent of this disease, of leishmania mexicana amazonensis, the etiological agent of cutaneous leishmaniasis, and of herpetomonas muscarum, a related non-pathogenic organism, all contain components which cause strong in vitro polyclonal activation of hamster spleen cells leading to the production o ... | 1985 | 3872189 |
| characterization of dolichol monophosphate- and dolichol diphosphate-linked saccharides in trypanosomatid flagellates. | dolichol-p- and dolichol-p-p-linked saccharides were isolated from several trypanosomatid flagellates incubated with [u-14c]glucose. formation of glc-p-dolichol and man-p-dolichol was observed in herpetomonas muscarum and leishmania adleri, whereas only the latter derivative was synthesized in trypanosoma dionisii and leptomonas samueli. the main and largest dolichol-p-p-linked oligosaccharide formed in trypanosoma conorhini, t. dionisii, l. samueli, herpetomonas samuelpessoai and h. muscarum ap ... | 1986 | 3083254 |
| a comparative study of the purine- and pyrimidine-metabolising enzymes of a range of trypanosomatids. | a range of trypanosomatids (amastigotes and cultured promastigotes of leishmania mexicana mexicana, cultured promastigotes of l. m. amazonensis, l. donovani and l. tarentolae, culture forms of crithidia fasciculata, herpetomonas muscarum muscarum and h. m. ingenoplastis and procyclic trypomastigotes of trypanosoma brucei brucei) have been surveyed for the presence of purine- and pyrimidine-metabolising enzymes. several common features were observed, including the presence of nucleosidases, catab ... | 1986 | 3015485 |