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cutaneous leishmaniasis of man due to leishmania (viannia) shawi lainson, de souza, póvoa, ishikawa & silveira, in pará state, brazil.leishmania (viannia) shawi lainson, braga, de souza, póvoa, ishikawa & silveira, 1989, was originally recorded from monkeys (cebus apella and chiropotes satanas), sloths (choloepus didactylus and bradypus tridactylus) and coatis (nasua nasua) and the sandfly, lutzomyia whitmani. with a panel of leishmania specific monoclonal antibodies, it was found that 30.5% of the leishmania strains from patients, who had contracted cutaneous leishmaniasis in pará state, were similar, but not identical to l. ...19911822654
leishmania (viannia) shawi sp. n., a parasite of monkeys, sloths and procyonids in amazonian brazil.leishmania (viannia) shawi sp. n., is described from the monkeys cebus apella and chiropotes satanus, the sloths choloepus didactylus and bradypus tridactylus, the procyonid nasua nasua, and the phlebotomine sandfly lutzomyia whitmani, all from primary forest in the state of pará, north brazil. l. (v.) shawi is variably distinguished from all other known species within the subgenus viannia by a combination of biological, biochemical and serological characters, as revealed by studies on morpholog ...19892504099
variation between geographical populations of lutzomyia (nyssomyia) whitmani (antunes & coutinho, 1939) sensu lato (diptera:psychodidae:phlebotominae) in brazil.phylogenetic analysis of morphometric and biological characters indicated that there are two distinct forms of lutzomyia whitmani in brazil: one is present both north and south of the river amazonas in the state of pará while the other occurs in northeast brazil, in the state of ceará, and further south, including the type locality in state of bahia. the amazonian form is reportedly neither strongly anthropophilic nor synanthropic, and it is the vector of leishmania shawi; whereas the southern f ...19968734947
species diversity of leishmania (viannia) parasites circulating in an endemic area for cutaneous leishmaniasis located in the atlantic rainforest region of northeastern brazil.to identify the aetiological agents of cutaneous leishmaniasis and to investigate the genetic polymorphism of leishmania (viannia) parasites circulating in an area with endemic cutaneous leishmaniasis (cl) in the atlantic rainforest region of northeastern brazil.200919708899
ex vivo and in vivo biological behavior of leishmania (viannia) shawi.since the first description of leishmania (viannia) shawi, few studies were performed with this parasite. in the present work, the in vivo and ex vivo behavior of l. (viannia) shawi infection was studied using murine model. peritoneal macrophages from balb/c and c57bl/6 mice were infected with promastigotes in the stationary phase of growth; after 24 h, the infection index and nitric oxide (no) levels in the supernatant of the cultures were analyzed. balb/c and c57bl/6 mice were infected into th ...200919756748
exacerbation of leishmania (viannia) shawi infection in balb/c mice after immunization with soluble antigen from amastigote forms.the present study aimed to evaluate the effects of immunization with soluble amastigote (amaag) and promastigote (proag) antigens from leishmania (viannia) shawi on the course of infection in balb/c mice. after immunization with amaag, the challenged group showed greater lesion size and parasite load in the skin and lymph nodes, associated with diminished interleukin (il)-2, il-4, il-10, interferon (ifn)-γ and nitrate levels in the supernatant of lymph node cell cultures, together with increases ...201021091779
phenotypic characterization of leishmania spp. causing cutaneous leishmaniasis in the lower amazon region, western pará state, brazil, reveals a putative hybrid parasite, leishmania (viannia) guyanensis × leishmania (viannia) shawi shawi.we phenotypically characterized 43 leishmanial parasites from cutaneous leishmaniasis by isoenzyme electrophoresis and the indirect immunofluorescence antibody test (23 mcabs). identifications revealed 11 (25.6%) strains of leishmania (v.) braziliensis, 4 (9.3%) of l. (v.) shawi shawi, 7 (16.3%) of l. (v.) shawi santarensis, 6 (13.9%) of l. (v.) guyanensis and l. (v.) lainsoni, 2 (4.7%) of l. (l.) amazonensis, and 7 (16.3%) of a putative hybrid parasite, l. (v.) guyanensis/l. (v.) shawi shawi. m ...201425083790
differential modulation of macrophage response elicited by glycoinositolphospholipids and lipophosphoglycan from leishmania (viannia) shawi.in this work, some aspects of the glycobiology of leishmania shawi were examined, as it is a causative agent of cutaneous leishmaniasis in the new world. additionally, the interaction of l. shawi's main glycoconjugates [lipophosphoglycan (lpg) and glycoinositolphospholipids (gipls)] with macrophages was evaluated in vitro. l. shawi lpg was devoid of side-chains in its repeat units, whereas monosaccharide analysis showed that gipls were suggestive of mannose-rich (type i or hybrid). in order to e ...201525619843
proteins of leishmania (viannia) shawi confer protection associated with th1 immune response and memory generation.leishmania (viannia) shawi parasite was first characterized in 1989. recently the protective effects of soluble leishmanial antigen (sla) from l. (v.) shawi promastigotes were demonstrated using balb/c mice, the susceptibility model for this parasite. in order to identify protective fractions, sla was fractionated by reverse phase hplc and five antigenic fractions were obtained.201222463817
characterization in vivo and in vitro of a strain of leishmania (viannia) shawi from the amazon region.the present study analyses complement resistance, cell surface carbohydrates expression, lipidic composition and morphology in vivo and in vitro, of leishmania (viannia) shawi, a parasite identified in the amazon region, pará state, in 1989. we demonstrated that promastigotes in the stationary (stat) growth phase are more resistant to complement lysis than in the logarithmic (log) growth phase. ultrastructural analyses and imidazol technique showed accumulation of lipids in stat growth phase pro ...200919567230
diagnostic efficacy of molecular techniques for detection and identification of leishmania species in human whole blood and skin samples from ecuador.microscopic examination is the standard method for diagnosis of cutaneous and mucocutaneous leishmaniasis despite its low sensitivity. this study compared the diagnosis efficacy of microscopic examination versus polymerase chain reaction (pcr)-based methods and dna sequencing using whole blood and skin lesion samples from patients with suspected leishmaniasis. the presence of leishmania was determined by microscopy and amplification of 18s ribosomal rna gene from blood and skin samples of 22 pat ...201627481055
in vitro cytokines profile and ultrastructural changes of microglia and macrophages following interaction with leishmania.in the present study, we assessed morphological changes and cytokine production after in vitro interaction with causative agents of american cutaneous leishmaniasis and compared the microglia and macrophage immune responses. cultures of microglia and macrophages infected with stationary-phase promastigotes of leishmania (viannia) shawi, leishmania (viannia) braziliensis or leishmania (leishmania) amazonensis were evaluated 24, 48 and 72 h after interaction. macrophages only presented the classic ...201424717447
Leishmania (Viannia) shawi purified antigens confer protection against murine cutaneous leishmaniasis.OBJECTIVE: Leishmania (Viannia) shawi was characterized only recently, and few studies concerning the immunogenic and protective properties of its antigens have been performed. The present study aimed to evaluate the protective potential of the five antigenic fractions isolated from L. (V.) shawi promastigotes in experimental cutaneous leishmaniasis. MATERIALS AND METHODS: Soluble antigen ...201122166919
Analysis of the protective potential of antigens released by Leishmania (Viannia) shawi promastigotes.Leishmania (Viannia) shawi causes cutaneous lesions in humans. Parasite antigens conferring significant protection against American tegumentar leishmaniosis (ATL) might be important for the development of effective vaccine. Therefore, this work evaluates the protective effect of antigenic fractions released by L. shawi. Antigens released by promastigotes to culture medium were concentrated and isolated by SDS-PAGE. The three main fractions LsPass1 (>75 kDa), LsPass2 (75-50 kDa) and LsPass3 (<50  ...201221882046
histopathology, humoral and cellular immune response in the murine model of leishmania (viannia) shawi.leishmania (viannia) shawi was recently characterized and few studies concerning modifications in cellular and humoral immune responses in experimental leishmaniasis have been conducted. in this work, immunopathological changes induced by l. shawi in chronically infected balb/c mice were investigated. infected balb/c mice developed increased lesion size associated with strong inflammatory infiltrate diffusely distributed in the dermis, with highly infected macrophages. the humoral immune respons ...201020060062
a mitochondrial dna phylogeny indicates close relationships between populations of lutzomyia whitmani (diptera: psychodidae, phlebotominae) from the rain-forest regions of amazônia and northeast brazil.phylogenetic analysis of all 31 described mitochondrial (cytochrome b) haplotypes of lutzomyia whitmani demonstrated that new material from the state of rondônia, in southwest amazônia, forms a clade within a lineage found only in the rain-forest regions of brazil. this rain-forest lineage also contains two other clades of haplotypes, one from eastern amazônia and one from the atlantic forest zone of northeast brazil (including the type locality of the species in ilhéus, state of bahia). these f ...199910419383
cutaneous leishmaniasis in northeastern brazil: a critical appraisal of studies conducted in state of pernambuco.american cutaneous leishmaniasis (acl) is a complex disease with clinical and epidemiological features that may vary from region to region. in fact, at least seven different leishmania species, including leishmania (viannia) braziliensis, leishmania (viannia) guyanensis, leishmania (viannia) lainsoni, leishmania (viannia) naiffi, leishmania (viannia) shawi, leishmania (viannia) lindenbergi, and leishmania (leishmania) amazonensis, have been implicated in the etiology of acl in brazil, and numero ...201222836662
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