Publications

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epidemiological and nosological aspects of leishmania naiffi lainson & shaw, 1989.leishmania naiffi was isolated from 10 out of 64 armadillos (dasypus novemcinctus) examined in amazonas, pará and rondônia states in the brazilian amazon region. the isolates were obtained in culture from samples of liver (3), spleen (3), lymph nodes (2), skin (1) and blood (1) from the infected animals. heavy infections with the same parasite were detected for the first time in psychodopygus squamiventris, a common man-biting phlebotomine, in amazonas and pará. a new case of cutaneous leishmani ...19911842423
monoclonal antibodies that react with leishmania (viannia) naiffi.monoclonal antibodies were raised against leishmania (viannia) naiffi, which recently has been characterized as a new species. balb/c mice were immunized with membrane-enriched fractions of a mixture of l. (v.) naiffi isolates. subsequent fusion of immunized splenocytes with ns-1 myeloma cells resulted in the production of 5 mabs (n1-n5). screening by elisa and indirect immunofluorescence against an extensive cross-panel of leishmania strains revealed that n3 was species-specific and could thus ...19911919913
cutaneous leishmaniasis of man due to leishmania (viannia) naiffi lainson and shaw, 1989.until recently mammalian hosts of leishmania (viannia) naiffi lainson & shaw, 1989 have been limited to armadillos, dasypus novemcinctus, in amazonian brazil. past evidence for human cutaneous leishmaniasis due to this parasite is reviewed, and a recent infection in man reported, with identification of the parasite by isoenzyme profiles and a specific monoclonal antibody. the role of some phlebotomine sandfly species as vectors is discussed.19902097934
leishmania (viannia) naiffi sp. n., a parasite of the armadillo, dasypus novemcinctus (l.) in amazonian brazil.a new leishmanial parasite, leishmania (viannia) naiffi sp. n., is described from the nine-banded armadillo, dasypus novemcinctus (edentata: dasypodidae), from para state, north brazil. the parasite grows luxuriantly in diffco blood-agar medium (b47), but poorly in the skin of intradermally inoculated hamsters. a comparison of isoenzyme profiles by starch gel electrophoresis separates the parasite from l. (v) braziliensis and l. (v.) guyanensis by the enzymes asat, alat, pgm, gpi, g6pd, pep, mpi ...19892930120
cutaneous leishmaniasis of humans due to leishmania (viannia) naiffi outside brazil. 19958560512
inhibition of macrophage invasion by monoclonal antibodies specific to leishmania (viannia) braziliensis promastigotes and characterisation of their antigens.monoclonal antibodies that specifically recognise leishmania (viannia) braziliensis promastigotes were produced and termed sst-2, sst-3 and sst-4. sst-2 recognises a conformational epitope present in a 24-28 kda doublet and in a 72 kda component, as verified by western blotting. indirect immunofluorescence showed that the antigen recognised by sst-2 is distributed homogeneously on the parasite surface. sst-3 recognises a flagellar glycoprotein of approximately 180 kda. the reactivity of this mab ...200111595232
human cutaneous leishmaniasis caused by leishmania naiffi is wide-spread in south america.four human cases of localized cutaneous leishmaniasis caused by leishmania naiffi are reported. two of the cases were infected in french guiana, one in french guiana or martinique, and the other in ecuador or peru. the geographical distribution of l. naiffi is clearly larger than that initially reported. three zymodemes were represented by the four isolates, confirming that there is intraspecific polymorphism in l. naiffi.200212625932
recent observations on the sand fly (diptera: psychodidae) fauna of the state of rondônia, western amazônia, brazil: the importance of psychdopygus davisi as a vector of zoonotic cutaneous leishmaniasis.sand flies were collected in the central region of the state of rondônia (w 64 degrees 30' to 63 degrees 00' and s 10 degrees 00'to 11 degrees 00') using shannon and cdc light traps from october 1997 to august 2000. a total of 85,850 specimens representing 78 named species were captured. of these 14 were new records for rondônia. the proportion of males/females was 1/1.131. trypanosomatids, that are presently being identified, were detected in 11 species. leishmania (viannia) naiffi was recorded ...200314595450
species diversity causing human cutaneous leishmaniasis in rio branco, state of acre, brazil.information on leishmania species diversity in western brazilian amazon and the clinical picture of human cutaneous leishmaniasis it causes is scarce. we describe clinical findings, diagnostic procedures and identification of leishmania species in patients from that region.200616930261
natural infection of lutzomyia tortura with leishmania (viannia) naiffi in an amazonian area of ecuador.natural infection of sand flies with leishmania parasites was surveyed in an amazonian area in ecuador where leishmaniasis is endemic. seventy-one female sand flies were dissected and one was positive for leishmania protozoa. the species of this sand fly was identified as lutzomyia (lu.) tortura on the basis of morphologic characteristics. analysis of the cytochrome b gene sequence identified the parasite as l. (viannia) naiffi. we report the distribution of l. (v.) naiffi in ecuador and detecti ...200818784239
[isoenzymatic characterization of human isolates of leishmania sp (kinetoplastida: trypanosomatidae) from the municipalities of rio preto da eva and manaus, state of amazonas].twenty-three isolates of leishmania sp from patients in the municipalities of rio preto da eva and manaus were characterized and identified by means of isoenzyme electrophoresis and the degree of similarity between the organisms was analyzed. the results indicated that leishmania guyanensis and leishmania naiffi were present in these two environments and that the leishmania naiffi samples were heterogenous.200819009197
leishmania spp. identification by polymerase chain reaction-restriction fragment length polymorphism analysis and its applications in french guiana.leishmania (viannia) guyanensis was for many years the only species commonly identified in french guiana, but precise species identifications were quite rare. we describe a new restriction fragment length polymorphism-polymerase chain reaction technique using a 615-bp fragment of the rna polymerase ii gene and 2 restriction enzymes, tspri and hgai. seven reference strains (leishmania (leishmania) amazonensis, leishmania (viannia) lainsoni, leishmania (viannia) braziliensis, l. (v.) guyanensis, l ...201019782495
spontaneous cure of american cutaneous leishmaniasis due to leishmania naiffi in two dutch infantry soldiers.we report two dutch infantry soldiers who acquired american cutaneous leishmaniasis (acl) during military jungle training in surinam. the lesions had existed for 3 and 5 months, respectively, before the soldiers presented for treatment. the lesions occurred on the head and right thigh, and were small, uncomplicated and symptomless. pcr for leishmania revealed leishmania naiffi in both patients. no treatment was given, and the lesions in both men healed spontaneously within 4 and 6 weeks, respect ...200920055858
lutzomyia sand fly diversity and rates of infection by wolbachia and an exotic leishmania species on barro colorado island, panama.sand flies (diptera, psychodidae, phlebotominae) in the genus lutzomyia are the predominant vectors of the protozoan disease leishmaniasis in the new world. within the watershed of the panama canal, the cutaneous form of leishmaniasis is a continuous health threat for residents, tourists and members of an international research community. here we report the results of screening a tropical forest assemblage of sand fly species for infection by both leishmania and a microbe that can potentially se ...201020231892
first cases of cutaneous leishmaniasis caused by leishmania (viannia) naiffi infection in surinam.cutaneous leishmaniasis in surinam is generally caused by infection by leishmania guyanensis. we report three cases of infection with leishmania (viannia) naiffi, a leishmania species not described from surinam before. treatment with pentamidine proved to be effective.201020348504
differences in the antigenic profile and infectivity of murine macrophages of leishmania (viannia) parasites.the antigenic profile and infectivity were compared between 3 recent leishmania (viannia) isolates from the amazonian region (instituto nacional de pesquisas da amazonia [inpa] strains) and 3 world health organization (who) reference species (leishmania guyanensis, leishmania braziliensis, and leishmania naiffi). differences were observed in the peak and extent of promastigote growth. the who reference strains exhibited significantly higher exponential growth as promastigotes than inpa strains. ...201020557195
occupationally acquired american cutaneous leishmaniasis.we report two occupationally acquired cases of american cutaneous leishmaniasis (acl): one accidental laboratory autoinoculation by contaminated needlestick while handling an acl lesion sample, and one acquired during field studies on bird biology. polymerase chain reaction (pcr) assays of patient lesions were positive for leishmania, subgenus viannia. one isolate was obtained by culture (from patient 2 biopsy samples) and characterized as leishmania (viannia) naiffi through an indirect immunofl ...201223227369
new primers for detection of leishmania infantum using polymerase chain reaction.leishmania infantum causes visceral leishmaniasis (vl) in the new world. the diagnosis of vl is confirmed by parasitological and serological tests, which are not always sensitive or specific. our aim was to design new primers to perform a polymerase chain reaction (pcr) for detecting l. infantum. sequences of the minicircle kinetoplast dna (kdna) were obtained from genbank, and the flc2/rlc2 primers were designed. samples of dna from l. infantum, leishmania amazonensis, leishmania braziliensis, ...201726603223
cutaneous leishmaniasis in northeastern brazil: a critical appraisal of studies conducted in state of pernambuco.american cutaneous leishmaniasis (acl) is a complex disease with clinical and epidemiological features that may vary from region to region. in fact, at least seven different leishmania species, including leishmania (viannia) braziliensis, leishmania (viannia) guyanensis, leishmania (viannia) lainsoni, leishmania (viannia) naiffi, leishmania (viannia) shawi, leishmania (viannia) lindenbergi, and leishmania (leishmania) amazonensis, have been implicated in the etiology of acl in brazil, and numero ...201222836662
leishmania (viannia) naiffi: rare enough to be neglected?in the brazilian amazon, american tegumentary leishmaniasis (atl) is endemic and presents a wide spectrum of clinical manifestations due, in part, to the circulation of at least seven leishmania species. few reports of leishmania (viannia) naiffi infection suggest that its occurrence is uncommon and the reported cases present a benign clinical course and a good response to treatment. this study aimed to strengthen the clinical and epidemiological importance of l. (v.) naiffi in the amazon region ...201526517660
ecological aspects of phlebotomine sand flies (diptera, psychodidae, phlebotominae) in areas of american cutaneous leishmaniasis, in the municipality of paraty, rio de janeiro, brazil. i-index of abundance by location and type of capture.the description of the first and autochthonous case of diffuse cutaneous leishmaniasis in the municipality of paraty, state of rio de janeiro, brought the interest of this study. sand flies were captured over a 3-yr period. using manual suction tubes, sand flies were collected from the inner and outer walls of homes, in the living spaces of domestic animals, and in shannon light traps, which were set up outside homes and in the forest. cdc light traps were installed inside homes, around the exte ...201526336245
hindii and sdui digests of heat-shock protein 70 pcr for leishmania typing.restriction fragment length polymorphisms of the heat-shock protein 70 gene have been used for discriminating leishmania species. here, we validated hindii as a much cheaper alternative to ecorii and sdui for discriminating leishmania (viannia) braziliensis from leishmania (viannia) naiffi and an atypical leishmania (v.) braziliensis group, which was previously not possible.201324050933
first human cases of leishmania (viannia) naiffi infection in ecuador and identification of its suspected vector species.epidemiological surveillance of leishmaniasis was conducted in a northern amazonian region of ecuador, in which cutaneous leishmaniasis cases were recently reported. sand flies were captured in the military training camp, and the natural infection of sand flies by leishmania species was examined. out of 334 female sand flies dissected, the natural infection by flagellates was microscopically detected in 3.9% of lutzomyia yuilli yuilli and 3.7% of lutzomyia tortura, and the parasite species were ...201324044975
diagnostic efficacy of molecular techniques for detection and identification of leishmania species in human whole blood and skin samples from ecuador.microscopic examination is the standard method for diagnosis of cutaneous and mucocutaneous leishmaniasis despite its low sensitivity. this study compared the diagnosis efficacy of microscopic examination versus polymerase chain reaction (pcr)-based methods and dna sequencing using whole blood and skin lesion samples from patients with suspected leishmaniasis. the presence of leishmania was determined by microscopy and amplification of 18s ribosomal rna gene from blood and skin samples of 22 pat ...201627481055
detection and identification of leishmania spp.: application of two hsp70-based pcr-rflp protocols to clinical samples from the new world.leishmaniasis is highly prevalent in new world countries, where several methods are available for detection and identification of leishmania spp. two hsp70-based pcr protocols (pcr-n and pcr-f) and their corresponding restriction fragment length polymorphisms (rflp) were applied for detection and identification of leishmania spp. in clinical samples recruited in colombia, guatemala, and honduras. a total of 93 cases were studied. the samples were classified into positive or suspected of leishman ...201728573463
natural leishmania (viannia) infections of phlebotomines (diptera: psychodidae) indicate classical and alternative transmission cycles of american cutaneous leishmaniasis in the guiana shield, brazil.from 1996 to 1999 multi-trapping methods (center of diseases control, cdc) light traps, light-baited shannon traps, and aspiration on tree bases) were used to study the phlebotomine fauna of the "serra do navio" region of the brazilian state of amapá, which is part of the guiana shield. fifty-three species were identified among 8,685 captured individuals. the following species, associated with the transmission of american cutaneous leishmaniasis in amazonian brazil, were captured: nyssomyia umbr ...201728508745
imported leishmaniasis in the netherlands from 2005 to 2012: epidemiology, diagnostic techniques and sequence-based species typing from 195 patients.leishmaniasis is an imported disease in the netherlands. we report data for the period between 2005 and 2012, on clinical presentation, country where leishmaniasis was acquired, and causative species, for 195 civilian and military patients who had travelled abroad. most patients were affected by cutaneous leishmaniasis (cl) (n=185 patients), while visceral leishmaniasis (vl) (n=8 patients) and mucocutaneous leishmaniasis (n=2 patients) were less frequently observed. all vl patients had been infe ...201323929178
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