| further observations on lutzomyia ubiquitalis (psychodidae: phlebotominae), the sandfly vector of leishmania (viannia) lainsoni. | | 1992 | 1343653 |
| cutaneous leishmaniasis in the amazon region: natural infection of the sandfly lutzomyia ubiquitalis (psychodidae: phlebotominae) by leishmania (viannia) lainsoni in pará state, brazil. | | 1991 | 1842393 |
| [cutaneous leishmaniasis in amazonia: isolation of leishmania (viannia) lainsoni from the rodent agouti paca (rodentia: dasyproctidae), in the state of pará, brazil]. | the isolation of leishmania (v.) lainsoni is recorded for the first time from a wild animal, the rodent agouti paca (rodentia: dasyproctidae), from pará state, north brazil. isolates of the parasite were made from apparently normal skin of 3 specimens of this rodent captured on the island of tocantins, in the municipality of tucuruí, an area subsequently flooded in the formation of the lake associated with the tucuruí hydroelectric dam. no isolations were made from the viscera. identification of ... | 1991 | 1843391 |
| [experimental cutaneous leishmaniasis. iii. histopathological aspects of the developmental behavior of the cutaneous lesion induced in cebus apella (primates: cebidae) by leishmania (viannia) lainsoni, l. (v.) braziliensis and l. (leishmania) amazonensis]. | we have studied the histopathological aspects related to the evolution of cutaneous lesions experimentally produced in the monkey cebus apella (primates: cebidae) by leishmania (v.) lainsoni, l. (v.) braziliensis and l. (l.) amazonensis. microscopical examination of a series of biopsies obtained from these animals showed the kinetics of the cutaneous lesions regarding three species of leishmania inoculated, as follows: 1) an initial non-specific chronic inflammatory infiltrate; 2) macrophagic no ... | 1990 | 2135483 |
| [experimental cutaneous leishmaniasis: i--on the susceptibility of the primate cebus apella (cebidae) to the infection caused by leishmania (viannia) lainsoni silveira, shaw and ishikawa, 1987]. | the susceptibility of the monkey cebus apella (cebidae) to experimental infection by leishmania (viannia) lainsoni has been investigated. for this purpose, five young monkeys, 2 males and 3 females, were intradermally, inoculated, in eight different places along the dorsal surface of the tail with 3 x 10(6) promastigotes of the parasite (mhom/br/81/m6426, benevides, pará), from stationary phase culture in difco b45 medium. after inoculations, infection in the monkeys was indicated by the presenc ... | 1989 | 2486523 |
| leishmania lainsoni: a peculiar viannia species. | | 1995 | 7741598 |
| leishmania (viannia) lainsoni occupies a unique niche within the subgenus viannia. | leishmania lainsoni has recently been recognized as a new peripylarian species belonging to the subgenus viannia and the l. braziliensis complex. it has been isolated from its sandfly vector, reservoir host and cutaneous lesions of human patients. microscopical examination has shown characteristics which are different from those of other members of the l. braziliensis complex. nuclear deoxyribonucleic acid (dna) hybridization patterns with a beta tubulin probe and kinetoplast dna buoyant density ... | 1995 | 7778160 |
| leishmania (viannia) lainsoni: first isolation in peru. | surveys were conducted from 1986 through 1992 to define the etiology and geographic distribution of human leishmaniasis in peru. lesion aspirates and skin biopsies were obtained from clinically diagnosed cases of leishmaniasis and tested for promastigotes by standard culture techniques. the isozyme profile of the isolates was determined by the cellulose acetate electrophoresis technique. data indicated that the isozyme profiles for leishmania isolates from six patients were similar to that of re ... | 1994 | 7985744 |
| kinetoplast minicircle dna from leishmania (viannia) lainsoni. | to investigate the phenomenon that pcr of leishmania (v.) lainsoni minicircles using primers b1 and b2 gives anomalous small-sized products, unlike all other members of the leishmania viannia subgenus, cloned kdna minicircles from l. (viannia) lainsoni were sequenced using fluorescent dye terminator reactions. the sequence of l. (v.) lainsoni where the primer b2 would be expected to bind, was different from the other members of the l. viannia subgenus, matching in only 7 out of 19 bases with the ... | 1999 | 10340327 |
| a first case of cutaneous leishmaniasis due to leishmania (viannia) lainsoni in bolivia. | we present the first known case of cutaneous leishmaniasis due to leishmania (viannia) lainsoni detected in bolivia. the parasite was isolated from a young girl living in the subtropical region of carrasco (900-1000 m above sea level, caranavi province, department of la paz, bolivia). the parasite identification was confirmed by multilocus enzyme electrophoresis. | 2001 | 11579876 |
| inhibition of macrophage invasion by monoclonal antibodies specific to leishmania (viannia) braziliensis promastigotes and characterisation of their antigens. | monoclonal antibodies that specifically recognise leishmania (viannia) braziliensis promastigotes were produced and termed sst-2, sst-3 and sst-4. sst-2 recognises a conformational epitope present in a 24-28 kda doublet and in a 72 kda component, as verified by western blotting. indirect immunofluorescence showed that the antigen recognised by sst-2 is distributed homogeneously on the parasite surface. sst-3 recognises a flagellar glycoprotein of approximately 180 kda. the reactivity of this mab ... | 2001 | 11595232 |
| first evidence of transmission of leishmania (viannia) lainsoni in a sub andean region of bolivia. | using ubiquitous primers which amplify the variable parts of kdna minicircle of all leishmania spp, we obtained for leishmania (viannia) lainsoni a major band of 605 bp (band 1) shared with l. v. braziliensis and a minor 524 bp band (band 2) specific of l. v. lainsoni. the specificity of the two bands was examined through southern blot hybridization of kdna pcr obtained from reference strains belonging to l. braziliensis, l. mexicana, l. donovani complexes with l. v. lainsoni species. band 1 was ... | 2002 | 12204398 |
| axenic promastigote forms of leishmania (viannia) lainsoni as an alternative source for leishmania antigen production. | the present study demonstrates that axenic cultures of leishmania (viannia) lainsoni produce larger cell masses in nnn-lit medium, as well as higher amounts of total proteins in cell extracts, than leishmania (leishmania) amazonensis. antigenicity of l. (v.) lainsoni whole promastigotes is similar to that of l. (l.) amazonensis, as demonstrated by an indirect immunofluorescence diagnostic test using sera from human patients and dogs infected with visceral leishmaniasis. infectivity of the l. (v. ... | 2005 | 16108545 |
| leishmania (viannia) lainsoni (kinetoplastida: trypanosomatidae), a divergent leishmania of the viannia subgenus--a mini review. | leishmania (viannia) lainsoni is the leishmania species that presents the most distinct biological (morphology, growth in axenic culture medium), biochemical (enzymatic electrophoresis profile), and molecular biology characteristics, when compared to other species of the viannia subgenus. development of promastigote forms of this parasite attached to the wall of the pyloric and hind gut regions of sand fly vectors is a solid characteristic that allows its positioning in the viannia subgenus. how ... | 2005 | 16302071 |
| species diversity causing human cutaneous leishmaniasis in rio branco, state of acre, brazil. | information on leishmania species diversity in western brazilian amazon and the clinical picture of human cutaneous leishmaniasis it causes is scarce. we describe clinical findings, diagnostic procedures and identification of leishmania species in patients from that region. | 2006 | 16930261 |
| leishmania (viannia) lainsoni (silveira et al. 1987): ultrastructural aspects of the parasite and skin lesion in experimentally infected hamster (mesocricetus auratus). | leishmania (viannia) lainsoni was first described in 1987 in the state of pará, in the brazilian amazon region. the initial characterization of this parasite was performed based only in light microscopy techniques. posterior studies with this leishmania species, which focused on biochemistry and molecular assays, showed its divergent position in the leishmania genus. in this study, we characterize the ultrastructure of culture promastigote forms throughout the growth curve. our results demonstra ... | 2007 | 17206507 |
| leishmania (viannia) lainsoni: occurrence of intracellular promastigote forms in vivo and in vitro. | experimental chronic (45-day-old) skin lesion in hamster hind foot induced by leishmania (viannia) lainsoni infection showed the presence of promastigote forms in the tissue, inside parasitophorous vacuoles, as assessed by transmission electron microscopy. experimental in vitro interaction (24 and 48 h) between leishmania (v.) lainsoni and j774-g8 macrophage cells also demonstrated the same profile. this morphological aspect is unusual, since in this parasite genus only amastigote forms have bee ... | 2006 | 17293989 |
| leishmania spp. identification by polymerase chain reaction-restriction fragment length polymorphism analysis and its applications in french guiana. | leishmania (viannia) guyanensis was for many years the only species commonly identified in french guiana, but precise species identifications were quite rare. we describe a new restriction fragment length polymorphism-polymerase chain reaction technique using a 615-bp fragment of the rna polymerase ii gene and 2 restriction enzymes, tspri and hgai. seven reference strains (leishmania (leishmania) amazonensis, leishmania (viannia) lainsoni, leishmania (viannia) braziliensis, l. (v.) guyanensis, l ... | 2010 | 19782495 |
| ecological aspects and molecular detection of leishmania dna ross (kinetoplastida: trypanosomatidae) in phlebotomine sandflies (diptera: psychodidae) in terra firme and várzea environments in the middle solimões region, amazonas state, brazil. | phlebotomine sand flies (diptera: psychodidae) are insects of medical importance due to the role that some species play in the transmission of leishmaniasis. this work aimed to study some ecological aspects among sand flies fauna inhabiting two different environments: the várzea (lowland amazonian forest) and terra firme (upland amazonian forest), both located in tefé municipality, amazonas state, braziland to detect leishmania infection in those phlebotomine populations. | 2015 | 25889808 |
| calmodulin polymerase chain reaction-restriction fragment length polymorphism for leishmania identification and typing. | a precise identification of leishmania species involved in human infections has epidemiological and clinical importance. herein, we describe a preliminary validation of a restriction fragment length polymorphism assay, based on the calmodulin intergenic spacer region, as a tool for detecting and typing leishmania species. after calmodulin amplification, the enzyme haeiii yielded a clear distinction between reference strains of leishmania mexicana, leishmania amazonensis, leishmania infantum, lei ... | 2016 | 27352873 |
| new primers for detection of leishmania infantum using polymerase chain reaction. | leishmania infantum causes visceral leishmaniasis (vl) in the new world. the diagnosis of vl is confirmed by parasitological and serological tests, which are not always sensitive or specific. our aim was to design new primers to perform a polymerase chain reaction (pcr) for detecting l. infantum. sequences of the minicircle kinetoplast dna (kdna) were obtained from genbank, and the flc2/rlc2 primers were designed. samples of dna from l. infantum, leishmania amazonensis, leishmania braziliensis, ... | 2017 | 26603223 |
| cutaneous leishmaniasis in northeastern brazil: a critical appraisal of studies conducted in state of pernambuco. | american cutaneous leishmaniasis (acl) is a complex disease with clinical and epidemiological features that may vary from region to region. in fact, at least seven different leishmania species, including leishmania (viannia) braziliensis, leishmania (viannia) guyanensis, leishmania (viannia) lainsoni, leishmania (viannia) naiffi, leishmania (viannia) shawi, leishmania (viannia) lindenbergi, and leishmania (leishmania) amazonensis, have been implicated in the etiology of acl in brazil, and numero ... | 2012 | 22836662 |
| first human cases of leishmania (viannia) lainsoni infection and a search for the vector sand flies in ecuador. | an epidemiological study of leishmaniasis was performed in amazonian areas of ecuador since little information on the prevalent leishmania and sand fly species responsible for the transmission is available. of 33 clinical specimens from patients with cutaneous leishmaniasis (cl), causative parasites were identified in 25 samples based on cytochrome b gene analysis. as reported previously, leishmania (viannia) guyanensis and l. (v.) braziliensis were among the causative agents identified. in addi ... | 2016 | 27191391 |