| presence and properties of thymidylate synthase in trypanosomatids. | high speed centrifugal supernatant fractions of homogenates of a number of trypanosomatids were assayed for thymidylate synthase (5,10-methylene-tetrahydrofolate: dump c-methyltransferase, ec 2.1.1.45) activity using the method of lomax and greenberg (1967) j. biol. chem. 242, 109-113). similar activities were detected in crithidia fasciculata, crithidia oncopelti, the blood forms of trypanosoma brucei, trypansoma congolense and trypanosoma lewisi and the blood, intracellular and culture forms o ... | 1977 | 14696 |
| physicochemical properties of two atypical cytochromes c, crithidia cytochrome c-557 and euglena cytochrome c-558. | cytochrome c-557 from crithidia oncopelti and cytochrome c-558 from euglena gracilis are mitochondrial cytochromes c that have an atypical haem-binding site. it was of interest to know whether the loss of one thioether bond affected the physicochemical properties of these cytochromes. the thermodynamic parameters of the redox potential were measured. the reaction with imidazole, the kinetics and thermodynamics of the alkaline isomerization and the effect of heating on the visible spectrum are de ... | 1975 | 242319 |
| the structure of animal mitochondrial dna (base composition, pyrimidine clusters, character of methylation). | base composition, content of pyrimidine isopliths and the degree of methylation of mitochondrial dna (mtdna) and nuclear dna (ndna) from various vertebrates and protozoon crithidia oncopelti have been studied. mtdnas from mammals (ox, rat) do not differ in fact in the gc content from the respective ndna. the gc content in mtdna from fishes (sheat fish) and birds (duck, chicken) is 1.5-2.5 mole % higher than in the respective ndna. kinetoplast dna (kdna) from crithidia oncopelti (gc = 42.9 mole % ... | 1977 | 323687 |
| the genetic system of kinetoplasts in trypanosomatides. | in the present report, the genetic system of crithidia oncopelti kinetoplast is used as a model for investigation of kinetoplast dna (kdna) structure, its transcription, protein synthesizing apparatus of the kinetoplast and the protein synthesis controlled genetically by kdna. it was shown that kdna of c. oncopelti can be isolated from cells or from kinetoplast fraction in the form of a network complex structure consisting of a lot of circular molecules. these minicircles have a contour length o ... | 1977 | 323689 |
| [heliomycin suppression of rna synthesis in a cell-free system]. | heliomycin inhibited in vitro the rna-polymerase reaction catalyzed by the preparation of dna-dependent rna-polymerase from e. coli. the blocking effect increased with a rise in the antibiotic concentration. the inhibitory effect of heliomycin decreased, when the amount of rna-polymerase in the system increased. yet, it did not depend on the content of dna and the nature of the dna preparation. preincubation of rna-polymerase with dna resulting in formation of the enzyme-matrix complex did not p ... | 1978 | 345959 |
| effects of methylglyoxal bis(ganylhydrazone) on trypanosomatid flagellates: inhibition of growth and nucleoside incorporation in trypanosoma brucei. | methyglyoxal bis (guanylhydrazone) (mgbg) at 0.5 mm had little effect in vitro on blastocrithidia culicis, crithidia oncopelti, and leishmania spp., but completely inhibited growth of trypanosoma brucei. inhibition became irreversible after a 3-h exposure of t. brucei culture procyclics. treated organisms remained motile, but failed to divide. polyamines, spermidine, and spermine, did not reverse the anti-trypanosome action of mgbg (preloading of cells or concurrent administration). two intraper ... | 1978 | 660567 |
| isolation of intracellular symbiotes by immune lysis of flagellate protozoa and characterization of their dna. | a new method dependent on immune lysis is described for the isolation of intracellular symbiotes from two species of flagellate protozoa blastocrithidia culicis and crithidia oncopelti. the symbiote-containing flagellates are exposed to complement and antisera prepared in rabbits against symbiote-free organisms. the immune lysis seems to weaken the plasma membranes of the flagellates so that subsequent application of gentle shearing force liberates the intracellular entities in an undamaged cond ... | 1975 | 805151 |
| structure of animal mitochondrial dna (base composition, pyrimidine clusters, character of methylation). | base composition, content of pyrimidine isopliths and methylation degree of mitochondrial dna (mtdna) and nuclear dna (ndna) from various vertebrates and protozoon crithidia oncopelti have been studied. mtdnas from mammals (ox, rat) do not differ in fact in the g + c content from the respective ndna. the g + c content in mtdna from fishes (sheat-fish) and birds (duck, chicken) is 1.5--2.5 mol % higher than in the respective ndna. kinetoplast dna (kdna) from crithidia oncopelti (g + c = 42.9 mol ... | 1977 | 843531 |
| symbiote-free hemoflagellates, blastocrithidia culicis and crithidia oncopelti: their liver factor requirement and serologic identity. | several aposymbiotic strains of blastocrithidia culicis and crithidia oncopelti were cultivated in trager's chemically defined medium as well as in a blood broth, both supplemented with 0.25% (v/v) liver extract concentrate. for all such strains, the liver extract was found to serve as an essential growth factor in the defined medium and as growth promoting additive in the blood broth. the active molecules were found to be water-soluble, heat stable, dialyzable, and probably nonlipid fractions. ... | 1976 | 933081 |
| structure of animal mitochondrial dna: nucleotide composition, pyrimidine clusters, and methylation character. | the nucleotide composition, relative concentration of pyrimidine clusters, and the degree of methylation of the mitochondrial and nuclear dna's of various vertebrates and the protozoan crithidia oncopelti have been studied. with respect to the relative concentration of gc pairs, the mtdna of animals (bull, rat) does not differ from the corresponding ndna. the relative concentration of gc pairs in the mtdna of certain fish and birds is 1.5-2.5 mole% higher than in the respective ndna. the kinetop ... | 1976 | 1023050 |
| purification and properties of nucleic acids from an unusual cytoplasmic organelle in the flagellate protozoan crithidia oncopelti. | a simple, rapid method for preparing bipolar bodies from sonicated (rithidia oncopelti cells, with a yield of 2-5%, is described. apart from 2-4% contamination with unbroken cells the fraction was considered pure with respect to contamination by other nucleic acid-containing organelles, as judged by light and electron microscopy. a light satellite dna, f bouyant density 1.695 g/ml in neutral cscl, and derived from the bipolar body, had a tm of 81.6 degrees c in0.15 m nacl/0.015 m sodium citrate ... | 1975 | 1098692 |
| [the suppression of the chemiluminescence of mammalian cells by the metabolites of crithidia oncopelti]. | effect of water-soluble (polypeptides) fraction (pf) of crithidia oncopelti on the chemiluminescence of human blood cells and murine splenocytes was studied. the study has shown that pf possessed a capacity to inhibit oxygen-dependent killer system of polymorphonuclear leucocytes in dose-dependent manner. this effect was of a complex nature. the inhibitory effect of pf was demonstrated also in respect to murine mononuclear phagocytes and splenocytes. it was suggested that, in this case, the supp ... | 1992 | 1452903 |
| [new biologically active substances from protozoa and an evaluation of their action]. | novel biologically active substances were obtained from nonpathogenic for humans and free-living protozoa: crithidia oncopelti, trypanosoma lewisi, and astasia longa. there were studied conditions of biosynthesis, composition and biological activity of the total lipid fraction from the protozoa species, sucrose ethers and fatty acids, the latter were isolated from a. longa--(astazilide preparation), reserve beta-1,3 glucan from a. longa (astazian preparation), and fraction of surface glycophosph ... | 1991 | 1809783 |
| homologies between paraflagellar rod proteins from trypanosomes and euglenoids revealed by a monoclonal antibody. | a nonidet p 40 insoluble fraction was isolated from trypanosoma brucei and was used to raise a monoclonal antibody (5e9). the antigen was localized by indirect immunofluorescence in the flagellum of t. brucei and of two species of euglenoids, euglena gracilis and distigma proteus. in immunoblot analysis, 5e9 appeared to bind to paraflagellar rod proteins pfr1 and pfr2 of t. brucei (72000 and 75000 mol. wt.) and of e. gracilis (67000 and 76000 mol. wt.). the presence of a common epitope in parafl ... | 1985 | 2581783 |
| kinetoplast dna segments function as promoters in escherichia coli cells. | by means of coupled transcription-translation in escherichia coli cell-free system, an open reading frame was found in the crithidia oncopelti maxi-circle kdna segment cloned in the hybrid plasmid pco1. subfragments from this region were tested for their ability to function as promoters in e. coli cells. for this purpose the vector pve8 was constructed using the aminoglycoside phosphotransferase ii (aptii) gene from the tn5 transposon, and the phc79 cosmid. after cloning of sau3a fragments of pc ... | 1985 | 2984521 |
| repeated sequences with unusual properties from the divergent region of crithidia oncopelti maxicircle kinetoplast dna. | the occurrence of bacterial promoter-like sequences was shown in the divergent region of crithidia oncopelti maxicircular kinetoplast dna. a promoter-cloning vector based on the neomycin phosphotransferase ii (npt ii) gene was used to localize promoters in three homologous blocks of repeated sequences. the elements found displayed greater promoter strength in escherichia coli than the normal promoter of the npt ii gene. sequences of three promoter-containing inserts from different blocks were de ... | 1986 | 3025554 |
| [autonomously replicating sequence activity of kinetoplast dna in yeast cells]. | different fragments of the maxicircle of the kinetoplast dna (kpdna) from crithidia oncopelti were cloned (fig. 1, 2) and tested for ars-activity (ars, autonomously replicating sequences). ars-activity was expressed as number of transformed yeast cells per microgram plasmid dna, as number of transformed cells per number of plasmid molecules (transformation efficiency, te) and as number of transformed cells per kilobase pair of cloned kpdna (specific transformation efficiency, tes) (fig. 3, table ... | 1986 | 3033193 |
| transcripts of the maxicircle kinetoplast dna of crithidia oncopelti. | functional organization of mitochondrial genome (maxicircle kinetoplast dna (kdna)) from a flagellate protozoan crithidia oncopelti was studied by northern hybridization. a set of overlapping transcripts were mapped in the conserved region of the maxicircle. several large (3-4 kb) rnas, overlapping two or more smaller transcripts were found. four regions produce a couple of rnas differing in size 50-100 bases. southern hybridization with several probes from the maxicircle kdna of leishmania tare ... | 1987 | 3431571 |
| recoveries of strigomonas oncopelti, leptospirae and reiter's treponeme from desiccates after storage for five years. | | 1965 | 5845600 |
| [divergent region of the maxicircle molecule of crithidia oncopelti kinetoplast dna contains ars elements]. | | 1984 | 6092023 |
| [isolation of ribonucleic acids possessing template activity from crithidia oncopelti mitochondria]. | three rna fractions (12se, 9se and 8se) were isolated from highly purified mitochondrial preparations of crithidia oncopelti. using inhibitory analysis and competitive hybridization, it was shown that the mitochondrial fractions under study are synthesized in the kinetoplasts and do not belong to ribosomal rna. it was demonstrated that these fractions possess template activity, since they stimulate protein synthesis in a cell-free system of e. coli. each fraction is subjected to translation to f ... | 1981 | 6170348 |
| evaluation of antigens for the serodiagnosis of kala-azar and oriental sores by means of the indirect immunofluorescence antibody test (ifat). | antigens and corresponding sera were collected from travellers with leishmaniasis returning to germany from different endemic areas of the old world. the antigenicity of these leishmania strains, which were maintained in syrian hamsters, was compared by indirect immunofluorescence (ifat). antigenicity was demonstrated by antibody titres in 18 sera from 11 patients. the amastigotic stages of nine strains of leishmania donovani and four strains of leishmania tropica were compared with each other a ... | 1981 | 6174454 |
| conservative and divergent base sequence regions in the maxicircle kinetoplast dna of several trypanosomatid flagellates. | the structure of kinetoplast maxicircle dna from trypanosomatids crithidia oncopelti, c. luciliae, leptomonas pessoai and leishmania gymnodactyli was compared by the blot hybridization method. the sizes of these molecules are 24.5, 34, 31 and 38 kilobase pairs (kbp), respectively. labelled maxicircle fragments from c. oncopelti were used as probes. a general model of the structural organization of maxicircles is proposed according to which this molecule is composed of a 17kbp conservative region ... | 1984 | 6207429 |
| purification and properties of a cross-linked complex between cytochrome c and cytochrome c peroxidase. | cytochrome c (horse heart) was covalently linked to yeast cytochrome c peroxidase by using the cleavable bifunctional reagent dithiobis-succinimidyl propionate in 5 mm-sodium phosphate buffer, ph 7.0. a cross-linked complex of molecular weight 48 000 was purified in approx. 10% yield from the reaction mixture, which contained 1 mol of cytochrome c and 1 mol of cytochrome c peroxidase/mol. of the total 40 lysine residues, four to six were blocked by the cross-linking agent. dithiobis-succinimidyl ... | 1982 | 6282263 |
| a cytochrome c methyltransferase from crithidia oncopelti. | the mitochondrial cytochrome c-557 of crithidia oncopelti contains two lysine residues and an n-terminal proline residue that are methylated in vivo by the methyl group of methionine. the purified cytochrome can act as a methyl acceptor for a methyltransferase activity in the cell extract that uses s-adenosylmethionine as methyl donor. crithidia cytochrome c-557 is by far the best substrate for this methyltransferase of those tested, in spite of the fact that methylation sites are already almost ... | 1982 | 6282265 |
| evidence for the presence of two terminal oxidases in the trypanosomatid crithidia oncopelti. | increasing concentrations of cyanide inhibited the respiration of whole cells of crithidia oncopelti in a biphasic fashion. approximately 80% inhibition was attained with 40 microm-kcn. no further inhibition occurred until the concentration of kcn reached approximately 200 microm. thereafter inhibition rose gradually to 100% at 1500 microm-kcn. difference spectra revealed the presence of two co-reacting haemoproteins. these were shown to be two different functional oxidases by photochemical acti ... | 1982 | 6288841 |
| [expression of the cloned fragments of maxiring kinetoplastic dna from crithidia oncopelti in a cell-free protein-synthesis system from escherichia coli]. | | 1983 | 6349958 |
| [polypeptide synthesis dependent on kinetoplast dna from crithidia oncopelti in a cell-free system of e. coli]. | the ability of a complex associate made up of maxi- and mini-circular molecules of kinetoplast dna from c oncopelti to perform polypeptide synthesis in a cell-free system of conjugate transcription and translation of e. coli was demonstrated. the coding function of maxi-circular molecules and genetic inertness of mini-circular molecules of kinetoplast dna during polypeptide synthesis in vitro was found. total rna from the kinetoplasts was shown to undergo translation in cell-free protein-synthes ... | 1981 | 7032609 |
| [cloning of the flagellate crithidia oncopelti on a solid nutrient medium of indeterminant composition not containing hemin]. | on a simple solid medium of indefinite content without hemin, colonies of crithidia oncopelti were received. the clonal nature of them was proved. the survival in some experiments varied from 10 to 70%. young colonies, 6 days after inoculation, were 0.3- 2.0 mm in diameter. c. oncopelti differed from herpetomonas megaseliae and crithidia harmosa in the morphology of colonies. to further genetical study of c. oncopelti, a possibility of cloning the object on a simple solid medium is an important ... | 1982 | 7041375 |
| [trypanosome sensitivity to polyene antibiotics]. | swelling of the plasma membrane is one of the mechanisms of resistance to damages in pathogenic protozoa. polyenic antibiotics induce reconstruction of the cytoplasma membrane of unicellular eukaryotic organisms, i. e. fungi and protozoa by binding the membrane lipids. the effect of 5 heptaene polyenic antibiotics, such as amphotericin b, mycoheptin, levorin, its sodium salt and levoridone on the growth of trypanosomides of trypanosoma lewisi and crithidia oncopelti was studied. the mic and ic50 ... | 1982 | 7051969 |
| characterization of kinetoplast dna from phytomonas serpens. | the restriction enzyme digestion of kinetoplast dna from four phytomonas serpens isolates shows an overall similar band pattern. one minicircle from isolate 30t was cloned and sequenced, showing low levels of homology but the same general features and organization as described for minicircles of other trypanosomatids. extensive regions of the minicircle are composed by g and t on the h strand. these regions are very repetitive and similar to regions in a minicircle of crithidia oncopelti and to ... | 1993 | 8389640 |
| analysis of ribosomal rna genes suggests that trypanosomes are monophyletic. | to further investigate the phylogeny of protozoa from the order kinetoplastida we have sequenced the small subunit (ssu) and a portion of the large subunit (lsu) nuclear rrna genes. the ssu and lsu sequences were determined from a lizard trypanosome, trypanosoma scelopori and a bodonid, rhynchobodo sp., and the lsu sequences were determined from an insect trypanosomatid, crithidia oncopelti, and a bodonid, dimastigella trypaniformis. contrary to previous results, in which trypanosomes were found ... | 1997 | 9115176 |
| the mitochondrial nd8 gene from crithidia oncopelti is not pan-edited. | rna editing in trypanosomatid mitochondria is a process involving the insertion and deletion of uridine residues within the coding region of maxicircle messenger rna transcripts. twelve of the 17 known genes need editing to produce functional molecules. we have analyzed the predicted editing sites for the crithidia oncopelti mitochondrial nadh-ubiquinone oxidoreductase subunit 8 (nd8) gene based on known mrnas from other trypanosomatid species. all studied nd8 mrnas undergo editing throughout th ... | 1998 | 9714563 |
| a giant phosphoprotein localized at the spongiome region of crithidia luciliae thermophila. | a giant protein with an apparent molecular mass of 2,300-kda was identified in the triton x-100 soluble fraction of crithidia luciliae thermophila. polyclonal antibody raised against this protein reacted by immunoblot analysis with proteins of similar molecular mass in crithidia fasciculata and crithidia oncopelti. in addition, the antibody immunoprecipitates the protein either after in vivo phosphorylation with [32p]orthophosphoric acid or after metabolically labeling with [35s]methionine. indi ... | 2000 | 11128704 |
| use of proteolytic enzymes as an additional tool for trypanosomatid identification. | the expression of proteolytic activities in the trypanosomatidae family was explored as a potential marker to discriminate between the morphologically indistinguishable flagellates isolated from insects and plants. we have comparatively analysed the proteolytic profiles of 19 monoxenous trypanosomatids (herpetomonas anglusteri, h. samuelpessoai, h. mariadeanei, h. roitmani, h. muscarum ingenoplastis, h. muscarum muscarum, h. megaseliae, h. dendoderi, herpetomoas sp., crithidia oncopelti, c. dean ... | 2005 | 15700759 |
| conserved repeats in the kinetoplast maxicircle divergent region of leishmania sp. and leptomonas seymouri. | the maxicircle control region [also termed divergent region (dr)] composed of various repeat elements remains the most poorly studied part of the kinetoplast genome. only three extensive dr sequences demonstrating no significant similarity were available for trypanosomatids (leishmania tarentolae, crithidia oncopelti, trypanosoma brucei). recently, extensive dr sequences have been obtained for leishmania major and trypanosoma cruzi. in this work we have sequenced dr fragments of leishmania turan ... | 2006 | 16909285 |
| From cryptogene to gene? ND8 editing domain reduction in insect trypanosomatids. | Mitochondrial ND8 gene is pan-edited in most known trypanosomatid species and 5'-edited only in a few trypanosomatid species. In this work, the ND8 nucleotide sequences from three species of insect trypanosomatids ("Wallaceina" sp. Wsd, Leptomonas rigidus and Leptomonas collosoma) were obtained and compared with previously known ND8 cryptogene sequences and mature mRNA sequences from other trypanosomatid species. We found a new pattern of editing: only 18 U residues are inserted in the 5' region ... | 2011 | 22014411 |
| purification, properties and amino acid sequence of atypical cytochrome c from two protozoa, euglena gracilis and crithidia oncopelti. | a basic cytochrome was isolated from the phytomastigophorean protozoan euglena gracilis and a similar protein from the zoomastigophorean protozoan crithidia oncopelti. in both cases chromatography on cm-cellulose in first the reduced and then the oxidized form proved to be an efficient means of purification. the two cytochromes can be classed in the cytochrome c family but they have certain atypical features. the alpha peak of the absorption spectrum is shifted towards the red and is asymmetrica ... | 2003 | 170910 |
| individual assignments of the heme resonances in the 360 mhz 1h nmr spectra of cytochrome c-557 from crithidia oncopelti. | with the use of nuclear overhauser effects, spin decoupling and saturation transfer experiments individual assignments for numerous resonances of the heme group in the 360 mhz 1h nmr spectra of reduced and oxidized cytochrome c-557 from crithidia oncopelti were obtained. these data provide direct evidence that the heme substituent in position 2 is a vinyl group. they further show that in spite of the different covalent structures of the heme groups the heme crevice and the electronic heme struct ... | 1997 | 229911 |
| structural studies by proton nmr of cytochrome c-557 from crithidia oncopelti. | | 2015 | 4356784 |
| phylogenetic validation of the genera angomonas and strigomonas of trypanosomatids harboring bacterial endosymbionts with the description of new species of trypanosomatids and of proteobacterial symbionts. | we comparatively examined the nutritional, molecular and optical and electron microscopical characteristics of reference species and new isolates of trypanosomatids harboring bacterial endosymbionts. sequencing of the v7v8 region of the small subunit of the ribosomal rna (ssu rrna) gene distinguished six major genotypes among the 13 isolates examined. the entire sequences of the ssu rrna and glycosomal glyceraldehyde phosphate dehydrogenase (ggapdh) genes were obtained for phylogenetic analyses. ... | 2011 | 21420905 |
| differential expression of proteolytic enzymes in endosymbiont-harboring crithidia species. | crithidia oncopelti, crithidia deanei and crithidia desouzai are flagellates of the trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. gelatin-sds-page analysis was used to characterize cell-associated and extracellular proteinases in these organisms. our survey indicates that the proteolytic profiles of c. deanei and c. desouzai are identical; that c. oncopelti displays a distinct zymogram; and that species naturally lacking endosymbionts have a more complex e ... | 2001 | 11506910 |
| lability of rna from the large cytoplasmic ribosomal subunit of the protozoon crithidia oncopelti. | cytoplasmic ribosomalrna extracted from crithidia oncopelti and analysed by gel electrophoresis at 4 degrees c consisted of two components, with molecular weights (relative to e. coli rrna) of 1-30 x 10(6) and 0-83 x 10(6) daltons, present in equimolar amounts. on heating briefly at 51 degrees c followed by rapid cooling, the 1-30 x 10(6) rna completely dissociated into two components of molecular weights 0-70 x 10(6) and 0-56 x 10(6) (present in equimolar amounts). fifty per cent dissociation o ... | 1976 | 944235 |
| sequences with high propensity to form g-quartet structures in kinetoplast dna from phytomonas serpens. | naturally occurring sequences containing repetitive guanine motifs have the potential to form tetraplex dna. phytomonas serpens minicircle dna shows some regions where one strand is composed mainly of g and t (gt regions). these regions contain several stretches of contiguous guanines. an oligonucleotide was constructed with the sequence corresponding to one of these regions (phyto-gt). it was demonstrated by native gel electrophoresis and methylation protection that phyto-gt forms tetramolecula ... | 1995 | 8538680 |
| reduced growth of blastocrithidia culicis and crithidia oncopelti freed of intracellular symbiotes by chloramphenicol. | the intracellular symbiotes of blastocrithidia culicis and crithidia oncopelti can be eliminated from cultures of the flagellates by a single chloramphenicol (cap) treatment. effective dosages were determined to be 0.01-0.08 per cent (w/v) cap after a treatment for 2 weeks or more for b. culicis and 0.08 per cent (w/v) after 1 month for c. oncopelti in most cases. ineffective dosages only lowered the numbers of symbiote-bearing flagellates. growth of both species of flagellates in the presence o ... | 1975 | 807721 |
| ultrastructure of symbiotic bacteria in normal and antibiotic-treated blastocrithidia culicis and crithidia oncopelti. | | 1974 | 4217371 |
| nutritional significance of symbiotic bacteria in two species of hemoflagellates. | symbiote-free strains of blastocrithidia culicis and crithidia oncopelti, obtained by chloramphenicol treatment, were compared nutritionally with normal, symbiote-containing strains. the symbiotic bacteria spare the flagellates requirements for exogenous hemin and for other nutritional factors present in liver extract. | 1974 | 4203488 |
| monophyly of endosymbiont containing trypanosomatids: phylogeny versus taxonomy. | to obtain additional information on the phylogenetic relationships within the family trypanosomatidae (order kinetoplastida), we have sequenced the small subunit ribosomal rna genes from the endosymbiont containing species herpetomonas roitmani tcc080, herpetomonas sp. tcc263, crithidia oncopelti atcc 12982 and a partial large subunit rrna gene from h. roitmani. the small subunit sequences in the two isolates of herpetomonas are very similar but not identical, and so are their restriction digest ... | 2006 | 9627990 |
| intracellular lectin-binding sites in symbiont-bearing crithidia species. | crithidia oncopelti, c. deanei, and c. desouzai are flagellates of the trypanosomatidae family that present bacterium-like endosymbionts in their cytoplasm. direct and indirect lectin-gold labeling techniques were used at the electron microscopic level in lowicryl k4m-embedded cells to demonstrate the presence of intracellular lectin-binding sites. we used the lectins ulex europaeus i, griffonia simplicifolia ii, ricinus communis i, arachis hypogaea, g. simplicifolia i, wistaria floribunda, limu ... | 1993 | 8278337 |
| [the trypanocidal action of fluorouracil on crithidia oncopelti in the presence of lipid metabolic modifiers]. | effect of fluorouracil in combination with ascorbate and l-valin, modifiers of lipid metabolism was studied in cultured protozoa crithidia oncopelti. the inhibitory effect of preparation in a concentration of 100 mcg/ml gradually decreased in the course of cultivation. its readdition to a 96-hour culture did not increase its effect on protozoa cells. combined addition of fluorouracil and modifiers (100 mcg/ml each) resulted in insignificant decrease of the cell accumulation in the culture as com ... | 2013 | 1795072 |
| [analysis of the sequence of repeats in divergent regions of maxi-circular dna from kinetoplasts of crithidia oncopelti]. | the nucleotide sequence of direct clustered repeats from the divergent region of the maxicircle kinetoplast (mitochondrial) dna from the protozoan crithidia oncopelti was analysed. 10 kbp long divergent region contains 3 clusters composed of 18-23 tandem repeats of 82-83 bp (sau-repeats) and a single cluster of five 417 bp repeats (ecori-repeats). the clusters are interspersed between the regions of nonrepetitive dna. the details of the structural organization of repeats and their clusters were ... | 2006 | 1965604 |
| characterization of kinetoplast minicircle dna in the lower trypanosomatid crithidia oncopelti. | the kinetoplast dna of the lower trypanosomatid crithidia oncopelti contains minicircles of four major size classes. attempts to clone these molecules revealed the unclonability of the full-length minicircles in puc and m13 vectors. a few clones were obtained using a lambda insertion vector lambda xiii. the nucleotide sequence of a cloned, apparently full-length minicircle has been determined and characterized. this 1848-bp molecule contains a single region corresponding to the 'conserved' minic ... | 1990 | 2166912 |
| the nucleotide sequence of the 12s ribosomal rna gene from kinetoplast dna of the protozoan crithidia oncopelti. | | 1990 | 2160071 |
| [composition and immunological activity of membrane-bound surface glycoprotein from crithidia oncopelti]. | a membrane-bound glycoprotein with a molecular weight of 10000-12000 was isolated from crithidia oncopelti and purified. the glycoprotein contained peptide, carbohydrate and lipid fragments and phosphorus. the peptide fragment was represented by 10 amino acids. the carbohydrate fragment was represented by 7 monosaccharides. the lipid part was mainly represented by stearic acid. the glycoprotein showed immunostimulating properties. it had a comitogenic effect on murine spleen cells in vitro and i ... | 1989 | 2633698 |
| [the effect of different antitumor preparations on the growth of a culture of the protozoan crithidia oncopelti]. | | 2008 | 2760329 |
| [the membrane-bound surface glycoprotein of crithidia oncopelti]. | membrane-associated surface glycoprotein from crithidia oncopelti (mag) has been isolated and studied. its molecular weight constitutes 10-12 kda. mag contains 12% protein, 29% carbohydrates, 1.2% phosphate and a lipid fragment. amino acid components of mag include glutamine, asparagine, alanine, proline, valine, leucine, serine, threonine, lysine, glycine. ethanolamine and glycerophosphate have also been found. mag contains stearic and small amounts of palmitic, oleic and myristic fatty acids. ... | 2008 | 2745860 |
| [initiation of transcription of the mitochondrial genome of the trypanosomatid crithidia oncopelti in vitro]. | | 1989 | 2556257 |
| cytoplasmic organelles of trypanosomatids: a cytochemical and stereological study. | the cytoplasmic organelles of different protozoa of the family trypanosomatidae were characterized by ultrastructural cytochemistry and stereology. data were obtained for mitochondria, lipid inclusions, glycosomes (peroxisome-like organelle), empty membrane-bounded vacuoles, reservosomes of trypanosoma spp., multivesicular body of crithidia fasciculata and dense granules of crithidia oncopelti. the stereological analysis (d = mean diameter, vv = volume density and nv = numerical density) was per ... | 1988 | 3135113 |
| flagellar wave reversal in the kinetoplastid flagellate crithidia oncopelti. | living crithidia oncopelti cells swim through their environment by means of tip-to-base waves on their single flagellum. the cells are able to re-orient themselves by using a short burst of asymmetrical base-to-tip waves. all points on a flagellum are capable of initiating waves. placing a population of cells in a medium of high viscosity initially produces a large number of organisms beating in the reverse mode. an individual cell has a random "switching" behavior. viscosity affects the frequen ... | 1988 | 3203154 |
| [presence of genetic exchange in crithidia oncopelti (trypanosomatidae) studied with drug resistance markers]. | strains resistant to 100 mkg/ml of cycloheximide (chxr100) and to 2.5 mg/ml of chloramphenicol (capr2.5) were used in studies for the presence of genetic interchange in c. oncopelti. phenotypes of these strains were stable on the nonselective medium and specific. strains chxr100 and capr2.5 were mixed in equal proportions, cultivated during 7 days and tested. under consecutive effect of inhibitors the culture of strains mixture and clones isolated from it exhibited double resistance. phenotypes ... | 2013 | 3481438 |
| [primary structure of an unusual gene for valine trna from crithidia oncopelti mitochondria]. | | 1987 | 2452063 |
| [effect of levorin and its combination with a modifying factor on lipid peroxidation in crithidia oncopelti]. | it was shown earlier by the authors that in the presence of sodium acetate as a modifier of c. oncopelti reaction to levorin, a polyenic antibiotic sensitivity of this organism to the antibiotic increased. at the same time there were observed changes in the ratio of the lipids and fatty acids of the total lipid fraction of c. oncopelti. the changes in the lipid composition of the biological membranes influenced lipid peroxidation (lpo). the present paper deals with investigation of interrelation ... | 1986 | 3717916 |
| [effect of levorin and its combination with a modifying factor on the fatty acid composition of crithidia oncopelti lipids]. | it was shown by us earlier that the sensitivity of crithidia oncopelti to levorin, a polyene antibiotic, increased in the presence of sodium acetate as a modifying factor. a simultaneous change in the ratio of c. oncopelti structural lipids was also observed. the present paper deals with investigation of the effect of levorin and its combination with the modifying factor on the fatty acid composition of c. oncopelti lipids. the study showed that addition of sodium acetate in a concentration of 4 ... | 1985 | 4026254 |
| [crithidia oncopelti (kinetoplastmonada) strains resistant to cycloheximide and chloramphenicol]. | by gradual (during several months) increase of the concentrations of cycloheximide (c) and chloramphenicol (ch) in cultural medium there were obtained strains of c. oncopelti resistant to the effect of 20, 50, 100 mkg/ml and 2.5 mg/ml ch. during 38, 28, 19 and 12 subculturings (that corresponds to 160, 120, 80 and 50 cell generations, respectively) on media without inhibitors parasites preserve their resistance to corresponding concentrations of c and ch without essential changes. cross resistan ... | 2013 | 4011312 |
| [ultrastructural changes in crithidia oncopelti as affected by levorin and its combination with a modifying factor]. | | 2013 | 3989096 |
| [the divergent region of kinetoplast maxi-ring dna in crithidia oncopelti contains 2 types of repetitive sequences]. | | 1985 | 4006688 |
| [effect of a modifying factor on the sensitivity of crithidia oncopelti to levorin]. | the paper deals with possible discovery of ways for increasing sensitivity of trypanosomides to polyenic antibiotics. the following substances were tested: sodium pyruvate and acetate, calcium salts, ascorbic acid and 1-valine. the total number of the cells and the number of the viable cells in the culture and their morphological characteristics were used as the criteria for estimation of the c. oncopelti sensitivity. it was shown that sodium acetate most actively modified the levorin effect on ... | 1984 | 6486748 |
| [effect of a modifying factor and its combination with levorin on the fractional lipid composition of crithidia oncopelti]. | | 2013 | 6480986 |
| [activation of lipid peroxidation in a crithidia oncopelti culture as a possible pathway for increasing drug sensitivity]. | | 2013 | 6747086 |
| [kinetoplast dna of crithidia oncopelti. recognition of the principles of structural organization of minicircular dna molecules]. | the heterogeneity of the kinetoplast minicircular dnas from crithidia oncopelti was studied by electron microscopy and restriction analysis. the associate of kinetoplast dna comprises five main classes of minicircles sizing 0.42, 0.51, 0.62, 0.78 and 0.83 microns. examination of cleavage patterns revealed that each class of the minicircles is heterogeneous in base sequence, but there are restriction fragments of the same size common for all these classes. a model of structural arrangement of min ... | 1984 | 6326864 |
| [ubiquinone-9 of the flagellates crithidia oncopelti and astasia longa]. | the content of ubiquinones (co q) of the astasia longa and crithidia oncopelti protozoa was studied. the protozoa were grown on an artificial nutrient broth. the cells were separated, washed, freeze-dried, and refluxed with koh and pyrogallol in ethanol media. the hydrolyzate was concentrated. the residue was stored at -20 degrees and filtered. an ubiquinone fraction was isolated from the filtrate by tlc on silica gel. identification of the ubiquinone homologues was carried out by reverse phase ... | 2016 | 6622442 |
| [ultrastructural changes in crithidia oncopelti as affected by olivomycin in the inhibition of intracellular lipid biosynthesis]. | | 2013 | 6619387 |
| [effect of modifying factors and their combination with olivomycin on the fatty acid composition of crithidia oncopelti lipids]. | | 2013 | 7119266 |
| [effect of modifying factors and their combinations with olivomycin on the lipid fractional composition of crithidia oncopelti]. | | 2013 | 7096776 |
| [effect of modifying factors on crithidia oncopelti sensitivity to olivomycin]. | | 1982 | 7076973 |
| motile flagellar axonemes with a 9 + 1 microtubule configuration. | electron microscope (em) studies of the eukaryotic flagellum reveal that the organelle contains a 9 + 2 arrangement of microtubules, the axoneme, with nine doublets surrounding two singlets enveloped by a membrane which is continuous with that of the cell; various linkages and projections are associated with the microtubules. strong experimental evidence supports the idea that the forces required for bend formation on eukaryotic flagella are derived from active relative sliding of the peripheral ... | 1980 | 6448961 |
| identification of n,n-dimethylproline as the n-terminal blocking group of crithidia oncopelti cytochrome c557. | the n-terminal tryptic peptide of crithidia oncopelti cytochrome c557 x-pro-me3lys-ala-arg in which x represents an unknown n-terminal blocking group was characterized by electrophoresis at ph 2 and by 1h and 13c nuclear magnetic resonance. 1h-nmr spectra of the tryptic peptide suggested that the blocking group x was n,n-dimethylproline although the electrophoretic mobility of the peptide suggested a larger molecular weight. the peptides x-pro-me3lys and x-pro were generated by treatment of the ... | 1980 | 6254758 |
| [presence of genes of ribosomal and transfer rnas in kinetoplast dna from two crithidia species]. | the coding properties of kinetoplast dna from two respresentatives of the order kinetoplastidae--crithidia oncopelti and c. fasciculata--were studied. experiments on hybridization of the whole network and fraction of minicircles with labelled 23s and 16s rrna and with trna isolated from kinetoplasts of c. oncopelti clearly demonstrated the presence of the genes for these rnas in the whole network and their absence in the minicircles. it may be thus concluded that the genes of ribosomal and trans ... | 1979 | 546450 |
| the ribosomal rna of the trypanosomatid protozoan crithidia fasciculata: physical characteristics and methylated sequences. | when extracted and analyzed under conditions which maintain noncovalently associated rna-rna complexes, the bulk cellular rna of crithidia fasciculata contains species of apparent molecular weights 1.3, 0.825, 0.08, 0.065, and 0.045 x 10(6) in addition to 5s rrna and trna. heat denaturation results in the disappearance of the 1.3 x 10(6) dalton rna and the appearance of three new species having molecular weights of 0.67, 0.575, and 0.059 x 10(6). in addition, the apparent molecular weight of the ... | 1979 | 476525 |
| [change in olivomycin sensitivity and in the lipid makeup of a crithidia oncopelti culture in the induction of the peroxidation of these compounds]. | | 1979 | 454723 |
| [dna-dependent rna polymerase activity of isolated zooflagellates (crithidia oncopelti) nucleoli]. | a fraction of nucleoli is isolated from zooflagellates (crithidia oncopelti) nuclei, its dna-dependent rna polymerase activity is studied at different temperature, ionic strength and mg2+, mn2+ and antibiotic concentrations. the effect of some factors and alpha-amantine on rna polymerase activity of exonucleolar chromatin was studied as a control. a comparison of heat denaturation of nucleoli and chromatin rna polymerase activities within the temperature range 30--55 degrees c has revealed a hig ... | 1978 | 565656 |
| [structural characteristics (reassociation and melting kinetics) of kinetoplast dna from crithidia oncopelti]. | a highly purified associate of kinetoplast dna is isolated from c. oncopelti, and its physico-chemical properties are studied. both native associate and its ultrasonic fragments are found to have a complex character of melting. 5-6 melting zones (3 of them being the main) are found on the melting curve. analysis of reassociation kinetics of sonicated associate of kinetoplast dna has revealed the presence of at least two components: fast reassociating component (65-70% of complex dna), which reas ... | 1977 | 562195 |
| [lipid makeup of crithidia oncopelti with a varying sensitivity to olivomycin]. | | 2013 | 1026745 |
| [dna-dependent rna-polymerase activity of isolated kinetoplasts of crithidia oncopelti]. | dna-dependent rna-polymerase activity was found in the kinetoplasts of crithidia oncopelti. kinetic patterns of 14c-utp incorporation into the acid-insoluble fraction of isolated kinetoplasts at 25 degrees, 30 degrees and 35 degrees c were estimated. the effects of different antibiotics and intercalating agents on rna synthesis in kinetoplasts were studied. alpha-amanitin, a specific inhibitor of the nuclear enzyme, does not affect the rna-polymerase activity of the kinetoplasts. streptolidigin ... | 1976 | 1035849 |
| effects of calcium on flagellar movement in the trypanosome crithidia oncopelti. | 1. the effects of calcium on the motility of different preparations of flagella from crithidia oncopelti were studied using stroboscopic and high-speed cine photographic techniques. 2. by varying the concentration of calcium in suspensions of chemically treated samples of the organism it was found that changes occurred in bend shape, wave direction and frequency. 3. waves on the flagellum of the organisms in vivo possess the unusual ability to propagate from tip to base, but reverse in direction ... | 1976 | 825606 |
| surface membrane carbohydrate alterations of a flagellated protozoan mediated by bacterial endosymbiotes. | crithidia oncopelti, a parasitic trypanosomatid protozoan of insects, normally contains intracellular symbiotic bacteria. as shown earlier, the protozoa can be rid of their endosymbiotes by chloramphenicol, producing a symbiote-free cell line. here surface-membrane carbohydrate ligands of the symbiote-containing and symbiote-free strains were compared by lectin-mediated agglutination, lectin-ultrastructure localization. [3h] lectin-binding, and fluorescent lectin staining. symbiote-free organism ... | 1976 | 1062797 |
| [action of acriflavine on the structure of the kinetoplast and its ribosomes in crithidia oncopelti]. | | 2013 | 1176703 |
| evidence for the amino-acid sequence of crithidia fasciculata cytochrome c555. | evidence is presented which indicates that the amino acid sequence of cytochrome c555 from crithidia fasciculata differs at sixteen positions from that of cytochrome c557 from crithidia oncopelti. 101 residues were identified by dansyl-edman degradation, carboxypeptidase digestion or considerations of the specificity of trypsin and of these, thirteen were found to be different from c. oncopelti cytochrome c557. the remaining 11 residues found in the amino acid composition of the trypic peptides ... | 1975 | 170111 |
| control of flagellar wave movement in crithidia oncopelti. | | 1975 | 1128679 |
| [some ultrastructural characteristics of crithidia oncopelti with varying sensitivity to olivomycin]. | | 2013 | 1133241 |
| [the relationship between crithidia oncopelti sensitivity to olivomycin and intracellular lipid concentration]. | | 2013 | 1117089 |
| [the lipid composition of the flagellate crithidia oncopelti cultivated on a peptone medium]. | | 2009 | 1117086 |
| [primary structure of dna from crithidia oncopelti kinetoplasts]. | | 1974 | 4426222 |
| [electron microscopic and biochemical study of the bipolar bodies in a crithidia oncopelti cell]. | | 2009 | 4377154 |
| [effect of antibiotics on the incorporation of c-14-leucine into ribosomal proteins of crithidia oncopelti kinetoplasts and cytoplasm]. | | 2013 | 4848569 |
| effects of pressure and temperature changes on the flagellar movement of crithidia oncopelti. | | 1974 | 4847274 |
| [the effect of antibiotics on the rna-polymerase activity of the nuclei of the zooflagellate crithidia oncopelti]. | | 1974 | 4463879 |
| micromanipulation of the flagellum of crithidia oncopelti. i. mechanical effects. | | 1974 | 4832989 |
| [isolation and properties of dna-dependent rna polymerase from nuclei of the flagellate crithidia oncopelti]. | | 1974 | 4442338 |
| [study of the ca2+-atpase of the flagella of strigomonas (crithidia) oncopelti]. | | 2013 | 4273595 |