| glucose-2-oxidase activity and accumulation of d-arabino-2-hexosulose in cultures of the basidiomycete oudemansiella mucida. | submerged cultures of the basidiomycete oudemansiella mucida, strain iii, accumulate d-arabino-2-hexosulose. the maximum yields during cultivations in shaker flasks or in a laboratory fermentor are 6--12 and 15 mg/ml, respectively (20--50% conversion of substrate glucose). the accumulation is transient, the aldoketose being again utilized after glucose exhaustion. its production is stimulated by fluoride ions. the enzyme responsible for the c(2)-specific oxidation of d-glucose acts as an intrace ... | 1978 | 689573 |
| laetiporus sulphureus causing visual hallucinations and ataxia in a child. | | 1988 | 3383041 |
| [stability kinetics of the exocellular cellulases in laetiporus sulphureus (b. ex fr.) bond. et sing., strain b-6]. | | 1980 | 7464575 |
| isolation and characterization of an n-acetyllactosamine-binding lectin from the mushroom laetiporus sulfureus. | a hemagglutinating and hemolytic lectin (psl) has been isolated from carpophores of the parasitic mushroom laetiporus sulfureus by affinity chromatography on sepharose. its molecular weight, as determined both by gel filtration and by electrophoresis in non-denaturing conditions, is about 190,000 and its structure is tetrameric, with two distinct types of subunits (about 60,000 and 36,000). it appeared homogeneous on hplc gel filtration but exhibited microheterogeneity on isoelectric focusing. h ... | 1994 | 7852268 |
| metabolism of pah by fungi and correlation with extracellular enzymatic activities. | the activity to metabolize the polycyclic aromatic hydrocarbons (pah) phenanthrene, anthracene, pyrene, fluorene and fluoranthene by trametes versicolor, pleurotus ostreatus (white rot fungi), laetiporus sulphureus, daedaela quercina, flamulina velutipes (brown rot fungi), marasmiellus sp. (litter decaying fungus) and penicillium sp. m 1 (isolated from a pah contaminated soil sample) were compared. screening methods for the presence of exoenzymes (peroxidases, polyphenoloxidases, "radical genera ... | 1993 | 8229670 |
| comparison of phenanthrene and pyrene degradation by different wood-decaying fungi. | the degradation of phenanthrene and pyrene was investigated by using five different wood-decaying fungi. after 63 days of incubation in liquid culture, 13.8 and 4.3% of the [ring u-14c]phenantherene and 2.4 and 1.4% of the [4,5,9,10-14c]pyrene were mineralized by trametes versicolor and kuehneromyces mutabilis, respectively. no 14co2 evolution was detected in either [14c]phenanthrene or [14c]pyrene liquid cultures of flammulina velutipes, laetiporus sulphureus, and agrocybe aegerita. cultivation ... | 1997 | 9327556 |
| a benzofuran glycoside and an acetylenic acid from the fungus laetiporus sulphureus var. miniatus. | a new benzofuran glycoside, masutakeside i (1) and a new c10 acetylenic acid, masutakic acid a (2) were isolated from the fruiting bodies of the fungus laetiporus sulphureus var. miniatus. their structures were established by spectroscopic and chemical methods. the known compounds 3-6 were also obtained and identified as egonol, demethoxyegonol, egonol glucoside and egonol gentiobioside. some of these compounds exhibited cytotoxicity against kato iii cells. | 2001 | 11253926 |
| [antimicrobial activity of laetiporus sulphureus strains grown in submerged culture]. | cultural conditions for growth and fruit body formation were elaborated to four strains of laetiporus sulphureus isolated from nature. all strains demonstrated antimicrobial activity against a wide spectrum of gram-positive and gram-negative bacteria during agar and submerged cultivation including methicillin-resistant strain of staphylococcus aureus (mrsa) and glycopeptide-resistant strain of leuconostoc mesenteroides. antifungal activity was not found. the level of antimicrobial activity durin ... | 2003 | 12741318 |
| molecular cloning, expression, and characterization of novel hemolytic lectins from the mushroom laetiporus sulphureus, which show homology to bacterial toxins. | we describe herein the cdna cloning, expression, and characterization of a hemolytic lectin and its related species from the parasitic mushroom laetiporus sulphureus. the lectin designated lsl (l. sulphureus lectin), is a tetramer composed of subunits of approximately 35 kda associated by non-covalent bonds. from a cdna library, three similar full-length cdnas, termed lsla, lslb, and lslc, were generated, each of which had an open reading frame of 945 bp encoding 315 amino acid residues. these p ... | 2003 | 12900403 |
| polysaccharides from the fruit bodies of the basidiomycete laetiporus sulphureus (bull.: fr.) murr. | the two main polysaccharides from the basidiomycetous fungus laetiporus sulphureus were isolated, purified and characterized. the structural assignments were carried out using (13)c, (1)h, and (1)h,(13) hsqc nuclear magnetic resonance spectroscopy, methylation analysis, and smith degradation. one was a linear beta-glucan having a (1-->3)-linked main chain, namely laminaran. the other was a fucomannogalactan, which consisted of a main chain of (1-->6)-linked alpha-d-galactopyranosyl residues, a p ... | 2004 | 14734165 |
| biologically active compounds from aphyllophorales (polypore) fungi. | this review describes biologically active natural products isolated from aphyllophorales, many of which are known as polypores. polypores are a large group of terrestrial fungi of the phylum basdiomycota (basidiomycetes), and they along with certain ascomycota are a major source of pharmacologically active substances. there are about 25 000 species of basidiomycetes, of which about 500 are members of the aphyllophorales, a polyphyletic group that contains the polypores. many of these fungi have ... | 2004 | 14987072 |
| crystallization and preliminary crystallographic analysis of a novel haemolytic lectin from the mushroom laetiporus sulphureus. | the novel haemolytic lectin from the parasitic mushroom laetiporus sulphureus (lsl) is a homotetramer (approximately 140 kda) composed of subunits associated by non-covalent bonds. it exhibits haemagglutination and haemolytic activities, both of which are inhibited by n-acetyllactosamine. the structural similarity found between lsl and the bacterial pore-forming toxins mosquitocidal toxin (mtx2) from bacillus sphaericus and alpha-toxin from clostridium septicum points to a mechanism of biologica ... | 2004 | 15159581 |
| screening of wood damaging fungi and macrofungi for inhibitors of pancreatic lipase. | a total of 120 methanol and dichloromethane extracts, from 60 species of wood-damaging fungi and 50 methanol/water extracts from macrofungi were screened for inhibition of pancreatic lipase using the chromogenic substrate p-nitrophenylpalmitate. of the extracts screened, those from laetiporus sulphureus, tylopilus felleus and hygrocybe conica exhibited the highest lipase inhibitory activities of 83% +/- 5%, 96% +/- 3% and 97% +/- 5%, respectively. | 2004 | 15478199 |
| antifungal activities of essential oils and their constituents from indigenous cinnamon (cinnamomum osmophloeum) leaves against wood decay fungi. | cinnamomum osmophloeum kaneh is one of the hardwood species indigenous to taiwan that possesses significant antifungal activity. to examine the antifungal activity of leaf essential oils and dominant constituents from c. osmophloeum, the essential oils of leaves from three clones (a, b, and c) collected from haw-lin experimental forest were extracted and their components analyzed by gas chromatography. results from the antifungal tests demonstrated that the essential oils of both b and c leaves ... | 2005 | 15607195 |
| antitermitic and antifungal activities of essential oil of calocedrus formosana leaf and its composition. | calocedrus formosana florin (cupressaceae) is an endemic tree species in taiwan; its timber is recognized for natural decay resistance. to examine the antitermitic and antifungal activities of leaf essential oil and its main constituents, c. formosana leaves were extracted and the essential oils analyzed by gc-ms. bioactivity tests against the termite coptotermes formosanus demonstrate that the lc50 value of leaf essential oil is 27.6 mg/g. furthermore, exposure to t-muurolol caused 100% mortali ... | 2004 | 15609830 |
| lanostanoid triterpenes from laetiporus sulphureus and apoptosis induction on hl-60 human myeloid leukemia cells. | a new lanostanoid triterpene, 3-oxosulfurenic acid (1), together with three known triterpenes (3, 4, and 7) were isolated from the fruit bodies of laetiporus sulphureus. cytotoxicity of these compounds and their derivatives (2, 5, and 6) was evaluated on hl-60 cells. further studies revealed that acetyl eburicoic acid (5) was the most potent apoptosis inducer. apoptosis was accompanied by both the activation of caspase-3 and the fragmentation of poly(adp-ribose) polymerase-1 and was also associa ... | 2004 | 15620242 |
| chemical composition and antifungal activity of essential oils from different tissues of japanese cedar (cryptomeria japonica). | in this study antifungal activities of essential oils from different tissues of japanese cedar (cryptomeria japonica d. don) against four wood decay fungi and six tree pathogenic fungi were investigated. in addition, the yields of essential oils obtained by water distillation were compared and their constituents determined by gc-ms analyses. the yield of essential oils from four tissues of japanese cedar is in the decreasing order of leaf (27.38 ml/kg) > bark (6.31 ml/kg) > heartwood (3.80 ml/kg ... | 2005 | 15686410 |
| structural analysis of the laetiporus sulphureus hemolytic pore-forming lectin in complex with sugars. | lsl is a lectin produced by the parasitic mushroom laetiporus sulphureus, which exhibits hemolytic and hemagglutinating activities. here, we report the crystal structure of lsl refined to 2.6-a resolution determined by the single isomorphous replacement method with the anomalous scatter (siras) signal of a platinum derivative. the structure reveals that lsl is hexameric, which was also shown by analytical ultracentrifugation. the monomeric protein (35 kda) consists of two distinct modules: an n- ... | 2005 | 15687495 |
| laetiporic acids, a family of non-carotenoid polyene pigments from fruit-bodies and liquid cultures of laetiporus sulphureus (polyporales, fungi). | the non-isoprenoid polyene laetiporic acid a, recently described from fruit-bodies of the wood-rotting fungus laetiporus sulphureus, was found to be the major orange pigment also in mycelium grown in liquid culture. its formation was variable, ranging from 0.1 to 6.7 mg/g dry weight in three strains, all of which were identified as l. sulphureus by its rdna sequence analysis. a second pigment, 2-dehydro-3-deoxylaetiporic acid a, is also described and fully characterized by nmr spectroscopy. two ... | 2005 | 15797608 |
| screening of selected wood-damaging fungi for the hiv-1 reverse transcriptase inhibitors. | extracts obtained using methanol and dichloromethane from 57 species of wood damaging fungi were investigated for their ability to inhibit hiv-1 reverse transcriptase activity in vitro using a non-radioactive assay. sixty-three samples were tested all together; some species were represented more than one isolate. thirteen methanolic extracts exhibited more than 40% inhibition and two among them inhibited the enzyme by more than 80%. all extracts obtained with dichloromethane were inferior to met ... | 2005 | 15907225 |
| n-phenethylhexadecanamide from the edible mushroom laetiporus sulphureus. | n-phenethylhexadecanamide was isolated from the meoh extract of the fruiting bodies of the mushroom laetiporus sulphureus together with eburicoic acid. their structures were elucidated by spectroscopic methods. | 2005 | 15938143 |
| modification of wood with isopropyl glycidyl ether and its effects on decay resistance and light stability. | china fir (cunninghamia lanceolata var. lanceolata) and maple (acer sp.) wood were etherified with isopropyl glycidyl ether and the decay resistance and light stability of the modified wood were assessed. cp/mas (13)c nmr and ft-ir analyses indicated that new ether bonds containing isopropyl groups formed after reacting wood with isopropyl glycidyl ether. modified wood samples were very resistant to decay when exposed to brown-rot fungus laetiporus sulphureus or white-rot fungus lenzites betulin ... | 2006 | 16039118 |
| chemical polymorphism and antifungal activity of essential oils from leaves of different provenances of indigenous cinnamon (cinnamomum osmophloeum). | the essential oils isolated from nine geographical provenances of indigenous cinnamon (cinnamomum osmophloeum kaneh.) leaves were examined by gc-ms and their chemical constituents were compared. according to gc-ms and cluster analyses the leaf essential oils of the nine provenances and their relative contents were classified into six chemotypes-cinnamaldehyde type, cinnamaldehyde/cinnamyl acetate type, cinnamyl acetate type, linalool type, camphor type and mixed type. in addition, the antifungal ... | 2006 | 16171686 |
| development of mushrooms for thrombosis prevention by protoplast fusion. | with thrombosis a major cause of death in japan and the western world, thrombin-inhibitory agents that constrain the formation of fibrin are sought. we screened for basidiomycetes showing anti-thrombin activity and isolated laetiporus sulphureus. however, it was difficult to cultivate and its form was not satisfactory. we therefore used protoplast fusion between l. sulphureus and the commonly cultivated basidiomycete hypsizygus marmoreaus to obtain cultivable basidiomycetes that produced an anti ... | 2000 | 16232780 |
| [bioactivity of laetiporus sulphureus var. sulphureus metabolites in liquid culture]. | the liquid culture of laetiporus sulphureus var. sulphureus was lethal against fruit fly. it was found that extracellular metabolites were primary causation of the lethal effect against fruit fly, which was influenced by ph value. isolation and analysis with ion-exchange resin column chromatography and hplc demonstrated that oxalic acid was present in supernatant of laetiporus sulphureus var. sulphureus, and it was one of the contributing factors to lethal effect against fruit fly and decrease o ... | 2005 | 16342759 |
| evaluation of antifungal properties of octyl gallate and its synergy with cinnamaldehyde. | the objective of this study was to investigate the possibility of using octyl gallate alone or with organic biocides as a preservative against wood decay fungi. antifungal activities of three antioxidants, propyl gallate, octyl gallate and butylated hydroxyltoluene (bht) were tested against four wood decay fungi, lenzites betulina, trametes versicolor, gloeophyllum trabeum and laetiporus sulphureus. octyl gallate was found to be the only active compound with ic50 values of 0.47, 0.16, 0.24 and 0 ... | 2007 | 16750625 |
| synergistic effects of cinnamaldehyde in combination with eugenol against wood decay fungi. | the combined effects of using cinnamaldehyde with catechin, quercetin or eugenol against wood decay fungi were examined by comparing their isoeffective concentrations to that of individual compound. among all combinations, cinnamaldehyde with eugenol revealed the strongest synergy against laetiporus sulphureus. the synergism was due to the interference of fungal cell wall synthesis and cell wall destruction plus radical scavenging effect. results also suggested that antioxidant with fungicidal e ... | 2008 | 17196382 |
| antifungal activity of cinnamaldehyde and eugenol congeners against wood-rot fungi. | in this study, the antifungal activities of cinnamaldehyde and eugenol congeners against white-rot fungus lenzites betulina and brown-rot fungus laetiporus sulphureus were evaluated and the relationships between the antifungal activity and the chemical structures were also examined. results from antifungal tests revealed that cinnamaldehyde, alpha-methyl cinnamaldehyde, (e)-2-methylcinnamic acid, eugenol and isoeugenol exhibited strong antifungal activity against all fungi tested. results derive ... | 2008 | 17945485 |
| production of extracellular polysaccharides by submerged mycelial culture of laetiporus sulphureus var. miniatus and their insulinotropic properties. | in the present study, optimum culture conditions for the production of extracellular polysaccharides (eps) in submerged culture of an edible mushroom, laetiporus sulphureus var. miniatus and their stimulatory effects on insulinoma cell (rinm5f) proliferation and insulin secretion were investigated. the maximum mycelial growth (4.1 g l(-1)) and eps production (0.6 g l(-1)) in submerged flask culture were achieved in a medium containing 30 g l(-1) maltose, 2 g l(-1) soy peptone, and 2 mm mnso(4).5 ... | 2008 | 18188554 |
| mutanase induction in trichoderma harzianum by cell wall of laetiporus sulphureus and its application for mutan removal from oral biofilms. | the cell wall material from fruiting bodies of laetiporus sulphureus has been suggested as a new alternative to mutan for the mutanase induction in trichoderma harzianum. structural analyses revealed that the alkali-soluble wall fraction from this polypore fungus contained 56.3% of (1-->3)-linked alpha-glucans. when the strain t. harzianum f-340 was grown on a cell wall preparation from l. sulphureus, the maximal enzyme productivity obtained after 3 days of cultivation was 0.71 u/ml. this yield ... | 2008 | 18667864 |
| [molecular characteristics of testosterone binding globulin and analysis of their regulation]. | molecular characteristics of testosterone binding globulin (tebg) were investigated in microaliquots of human serum samples using 3h-5-alpha-dihydrotestosterone radioligand assays. under experimental conditions used tebg demonstrated high conformational plasticity and ability to adopt three conformational states (olygo-, di-, and monomer) with different functional activities. using alipidspolyene complex from basidiomycete laetiporus sulphureus we demonstrate a novel effective pharmacological co ... | 2008 | 18712090 |
| genetic variation and relationships in laetiporus sulphureus s. lat., as determined by its rdna sequences and in vitro growth rate. | the aim of this study was to characterise the genetic variation and molecular relationships of the brown rot polypore, laetiporus sulphureus s. lat., from europe, south america, africa, and asia, using its sequences of the nu-rdna and by comparing the growth rate in vitro. in a nj analysis of the sequences of 130 individuals of l. sulphureus s. lat., eight distinct clusters emerged, supported by bs values of 70-100%. within each cluster, the its rdna sequence variation was below 3%. the sequence ... | 2009 | 19073254 |
| purification and characterization of a thermostable xylanase from the brown-rot fungus laetiporus sulphureus. | a thermostable extracellular xylanase was purified and characterized from brown-rot basidiomycete laetiporus sulphureus, cultivated on biologically pretreated pinus densiflora biomass. after three consecutive purification steps using deae, mono q, and superdex 75 columns, the xylanase specific activity was found to be 72.4 u/mg, nine fold higher than that of the crude culture solution, purity was 96%, and the molecular mass determined to be 69.3 kda. the optimal ph and temperature for xylanase a ... | 2009 | 19147106 |
| purification and characterization of a thermostable beta-1,3-1,4-glucanase from laetiporus sulphureus var. miniatus. | a beta-1,3-1,4-glucanase from the fungus laetiporus sulphureus var. miniatus was purified as a single 26 kda band by ammonium sulfate precipitation, hitrap q hp, and uno q ion-exchange chromatography, with a specific activity of 29 u/mg. the molecular mass of the native enzyme was 52 kda as a dimer by gel filtration. beta-1,3-1,4-glucanase showed optimum activity at ph 4.0 and 75 degrees . the half-lives of the enzyme at 70 degrees and 75 degrees were 152 h and 22 h, respectively. the enzyme sho ... | 2009 | 19734720 |
| the genus laetiporus (basidiomycota, polyporales) in east asia. | relationships among east asian, north american and european laetiporus sulphureus s. lat., a cosmopolitan brown rot species complex, were assessed with phylogenetic analyses and incompatibility tests. three east asian taxa, laetiporus cremeiporus sp. nov., laetiporus montanus and laetiporus versisporus, are described and illustrated as well as compared with related taxa from southeast asia, north america and europe. phylogenetic analyses showed that l. cremeiporus and l. versisporus are clearly ... | 2009 | 19769929 |
| laetirobin from the parasitic growth of laetiporus sulphureus on robinia pseudoacacia. | (+/-)-laetirobin (1) was isolated as a cytostatic lead from laetiporus sulphureus growing parasitically on the black locust tree, robinia pseudoacacia, by virtue of a reverse-immunoaffinity system. using an lc/ms procedure, milligram quantities of (+/-)-laetirobin (1) were obtained, and the structure of 1 was elucidated by x-ray crystallography and confirmed by nmr spectroscopy. preliminary cellular studies indicated that (+/-)-laetirobin (1) rapidly enters in tumor cells, blocks cell division a ... | 2009 | 19842686 |
| [water-soluble endopolysaccharides from the fruiting bodies of laetiporus sulphureus (bull.: fr.) murr]. | endopolysaccharides represented by glucans, galactans, and glycoproteins were isolated from the fruiting bodies of the sulfur shelf (laetiporus sulphureus (bull.:fr.) murr), which were obtained by the natural-plantation method. the study of the major 56-kda polysaccharide, laetiporan a, the content of which accounted for 0.28% of the fruiting body weight, showed that it is a beta-1,3-glucan containing mannose, galactose, fucose, xylose, and rhamnose residues at position c-6. an antioxidant effec ... | 2009 | 19845294 |
| [alkali-soluble polysaccharides of laetiporus sulphureus (bull.: fr.) murr fruit bodies]. | alkali-soluble polysaccharides have been extracted from laetiporus sulphureus (bull.: fr.) murr fruit bodies with a yield of 42.7%. the structure of the dominant polymer (16.05% of fruit bodies' mass), named latiglucan i, has been determined. it is linear beta-1,3-glucan (molecular weight 1.8 x 10(5) da, [alpha]d-17 degrees). | 2009 | 20067155 |
| the effect of ionising radiation on testosterone binding globulin characteristics: correction of the protein' parameters by lipid polyene complexes of fungus laetiporus sulfureus. | the aims of this work were: (i) to compare the effects of ionising radiation (ir) on testosterone binding globulin (tebg) characteristics (serum concentration, cooperativity of androgen binding and affinity for hormone) in divergent mammalian species; (ii) to couple radiation effects with probable tebg-parameter changes; and (iii) to investigate the prevention of these changes by fungal preparations (in particular - by lipid polyene complexes of laetiporus sulphureus). | 2010 | 20201652 |
| mutanase from paenibacillus sp. mp-1 produced inductively by fungal α-1,3-glucan and its potential for the degradation of mutan and streptococcus mutans biofilm. | laetiporus sulphureus is a source of α-1,3-glucan that can substitute for the commercially-unavailable streptococcal mutan used to induce microbial mutanases. the water-insoluble fraction of its fruiting bodies from 0.15 to 0.2% (w/v) induced mutanase activity in paenibacillus sp. mp-1 at 0.35 μ ml(-1). the mutanase extensively hydrolyzed streptococcal mutan, giving 23% of saccharification, and 83% of solubilization of glucan after 6 h. it also degraded α-1,3-polymers of biofilms, formed in vitr ... | 2010 | 20623316 |
| laetiporus sulphureus lectin and aerolysin protein family. | the parasitic mushroom laetiporus sulphureus produces a family of lectins (lsl's) sharing 80-90% sequence identity that possesses a low but significant sequence similarity to the bacterial pore-forming toxins mosquitocidal toxin mtx-2 from bacillus sphaericus and a toxin from clostridium septicum. the crystal structure of one member of the l. sulphureus lectins family (lsla) reveals unexpected structural similarities to the 1-pore-forming toxins from the aerolysin family, namely, aerolysin from ... | 2010 | 20687481 |
| medicinal and edible lignicolous fungi as natural sources of antioxidative and antibacterial agents. | the antioxidant activity of organic extracts of eight fungal species, ganoderma lucidum, ganoderma applanatum, meripilus giganteus, laetiporus sulphureus, flammulina velutipes, coriolus versicolor, pleurotus ostreatus and panus tigrinus, was evaluated for free radical (dpph· and oh·) scavenging capacity and an effect on lipid peroxidation, and the antibacterial activity was tested by the agar well diffusion method. the highest dpph· scavenging activity was found in the methanol extract of g. app ... | 2010 | 20878697 |
| α-(1 → 3)-d-glucans from fruiting bodies of selected macromycetes fungi and the biological activity of their carboxymethylated products. | purpose of work: to show biological activity of carboxymethylated α-(1 → 3)-d-glucans isolated from the selected macromycetes fungi on human tumor and normal cells. water-insoluble, alkali-soluble polysaccharides (wip) were isolated from fruiting bodies of four macromycetes fungi: lentinus edodes, pleurotus ostreatus, piptoporus betulinus and laetiporus sulphureus. the structure of the polysaccharides was determined using composition analysis, methylation analysis, fourier transform infrared spe ... | 2010 | 21188616 |
| initial acidic ph is critical for mycelial cultures and functional exopolysaccharide production of an edible mushroom, laetiporus sulphureus var. miniatus jm 27. | we conducted a time course experiment on mycelial cultures of laetiporus sulphureus var. miniatus. the strain showed significant survival in an initial ph range of 2.0 to 7.0 for 24 days, during which time oxalic acid was accumulated. a structural analysis of purified exopolysaccharide suggested that it contained 96.1% glucose, and the mode of linkage was mainly → 4-glcp-(1 → units, with branches at the c-6 position consisting of a glcp-(1 → 4) linked side chain. an exopolysaccharide purified fr ... | 2010 | 21221951 |
| crystal structure of clostridium perfringens enterotoxin displays features of {beta}-pore-forming toxins. | clostridium perfringens enterotoxin (cpe) is a cause of food poisoning and is considered a pore-forming toxin, which damages target cells by disrupting the selective permeability of the plasma membrane. however, the pore-forming mechanism and the structural characteristics of the pores are not well documented. here, we present the structure of cpe determined by x-ray crystallography at 2.0 å. the overall structure of cpe displays an elongated shape, composed of three distinct domains, i, ii, and ... | 2011 | 21489981 |
| molecular basis of toxicity of clostridium perfringens epsilon toxin. | clostridium perfringensε-toxin is produced by toxinotypes b and d strains. the toxin is the aetiological agent of dysentery in newborn lambs but is also associated with enteritis and enterotoxaemia in goats, calves and foals. it is considered to be a potential biowarfare or bioterrorism agent by the us government centers for disease control and prevention. the relatively inactive 32.9 kda prototoxin is converted to active mature toxin by proteolytic cleavage, either by digestive proteases of the ... | 2011 | 21518257 |
| high-resolution structural insights on the sugar-recognition and fusion tag properties of a versatile {beta}-trefoil lectin domain from the mushroom laetiporus sulphureus. | in this work, we analyzed at high-resolution the sugar-binding mode of the recombinant n-terminal ricin-b domain of the haemolytic protein lsla (lsl(150)) from the mushroom laetiporus sulphureus, and also provide functional in vitro evidences suggesting that, together with its putative receptor-binding role, this module may also increase the solubility of its membrane pore-forming partner. we firstly demonstrate that recombinant lsl(150) behaves as an autonomous folding unit and an active lectin ... | 2011 | 21632870 |
| exopolysaccharide of laetiporus sulphureus var. miniatus downregulates lps-induced production of no, pge(2), and tnf-+¦ in bv2 microglia cells via suppression of the nf-+¦b pathway. | our previous study showed that the exopolysaccharide (eps) of laetiporus sulphureus var. miniatus was well characterized and prevented cell damage in streptozotocin-induced apoptosis. however, little is known about the molecular mechanisms underlying its anti-inflammatory effects. therefore, we attempted in this study to determine whether eps induces a significant inhibition of pro-inflammatory mediators in lipopolysaccharide (lps)-stimulated murine bv2 microglia cells. our results showed that e ... | 2011 | 21843581 |
| phytochemicals from cunninghamia konishii hayata act as antifungal agents. | the aims of the present study were to isolate and identify the antifungal compounds from the ethanolic extract of cunninghamia konishii wood and to evaluate their antifungal activities against wood decay fungi. the results showed that the n-hex soluble fraction of the ethanolic extract from c. konishii wood had an excellent inhibitory effect against lenzites betulina, trametes versicolor, laetiporus sulphureus, and gloeophyllum trabeum, with ic(50) values of 33, 46, 62, and 49 μg/ml, respectivel ... | 2011 | 22129092 |
| biochemical characterization of the exopolysaccharide purified from laetiporus sulphureus mycelia. | the extracellular polysaccharide (eps) was isolated from mycelial cultures of laetiporus sulphureus var. miniatus and purified by deae cellulose and sephadex g-50 column chromatography. the purified eps (eps-2-1) was composed of only glucose units and its molecular mass was 6.95 kda. the chemical structure of eps-2-1 consisted of a main chain containing (1-->4)-glcp units with branches at the c-6 position of the chain carrying -glcp-(1-->4)-linked residues. the effect of purified eps on immunomo ... | 2011 | 22210615 |
| enzymatic saccharification of biologically pretreated pinus densiflora using enzymes from brown rot fungi. | enzymatic saccharification of lignocellulosic biomass was performed using culture filtrates of brown rot fungi including gloeophyllum sepiarium, fomitopsis pinicola, and laetiporus sulphureus. biological treatment with white rot fungi was used as pretreatment prior to enzymatic saccharification. endoglucanase, beta-glucosidase, xylanase and cellobiohydrolase activities were determined from concentrated culture filtrates of the brown rot fungi. l. sulphureus has the highest endoglucanase, beta-gl ... | 2008 | 18804059 |
| [antioxidant components of laetiporus sulphureus (bull.: fr.) murr. fruit bodies]. | antioxidant activity of fruit bodies of laetiporus sulphureus (bull.: fr.) murr. (polyporales) obtained by the natural plantation growing method in pribaikal'e (irkutsk region) has been studied. it was determined that the ethyl acetate fraction of l. sulphureus, which was chromatographically separated into seven compounds identified as quercetin, kaempferol, (+)-catechin, p-coumaric, gallic, caffeic, and chlorogenic acids was characterized with more expressed antioxidant activity. all compounds ... | 2011 | 21950122 |
| the nutritional composition of selected wild edible mushrooms from sicily (southern italy). | the wide consumption of wild fungi by the local population is the basis for an evaluation of the as yet unknown nutritional composition of some selected species. wild edible mushrooms (fistulina hepatica, infundibulicybe geotropa, laetiporus sulphureus, macrolepiota procera var. procera and suillus granulatus) were collected in a number of sicilian (southern italian) forest ecosystems and analysed according to standard methodologies of fungal analysis, for their basic composition (ash, water, pr ... | 2011 | 21749273 |
| [melanin of laetiporus sulphureus (bull.: fr.) murr sterile form]. | melanin complex was isolated from mycelium of the basidiomycete laetiporus sulphumreus (bull.: fr.) murr (with a yield of 2.49% of the fresh weight). uv and ir spectroscopies, gel chromatography, and alkaline cleavage assay demonstrated that the isolated melanin was heterogeneous and belonged to the dihydronaphthalene type. 13c-nmr data suggested that aromatic fragments were dominant in the melanin structure. in vitro study of the antioxidant action demonstrated that the l. sulphureus melanin di ... | 2011 | 21790034 |