| degradation of 4-hydroxyphenylacetic acid by trichosporon cutaneum. | trichosporon cutaneum degraded 4-hydroxyphenylacetic acid to acetoacetic and malic acids. 3.4-dihydroxyphenylacetic acid, an intermediate in the reaction sequence, underwent hydroxylation before the benzene ring was cleaved. | 1978 | 30749 |
| metabolism of phenol and resorcinol in trichosporon cutaneum. | trichosporon cutaneum was grown with phenol or resorcinol as the carbon source. the formation of beta-ketoadipate from phenol, catechol, and resorcinol was shown by a manometric method using antipyrine and also by its isolation and crystallization. metabolism of phenol begins with o-hydroxylation. this is followed by ortho-ring fission, lactonization to muconolactone, and delactonization to beta-ketoadipate. no meta-ring fission could be demonstrated. metabolism of resorcinol begins with o-hydro ... | 1979 | 33145 |
| trichosporon sepsis and leukemia. | trichosporon cutaneum is a fungus known to cause superficial nodules over the distal third of hair shafts, mainly scalp hair, and to produce a clinical entity known as piedra. this superficial mycosis occurs mostly in temperate and tropical regions and is rarely seen in north america. trichosporon cutaneum spesis is described here in a 12-year-old boy with acute lymphocytic leukemia in relapse. to our knowledge this is the first case reported in the literature. emphasis is made of the increasing ... | 1975 | 52400 |
| [recent causal organisms of bacteremia and a case of septicemia caused by trichosporon cutaneum (author's transl)]. | | 1978 | 101615 |
| the distribution of pyrophosphatidic acid in nature. | the occurrence of a novel phospholipid, pyrophosphatidic acid, in the lipid extracts of yeasts (23 species), bacteria (e. coli), algae (chlorella), mammalia (human, rabbit, guinea pig, and mouse), insect (cockroach), fish (carp), mollusc (clam), and spermatophyta (spinach) was investigated. pyrophosphatidic acid was found exclusively in the lipid extracts of several kinds of yeast species, but not in other normal living species (animals, plants, and microorganisms) so far investigated. all of th ... | 1975 | 175047 |
| the enzymes of ammonia assimilation in trichosporon cutaneum. | | 1975 | 239097 |
| diagnostic medium containing inositol, urea, and caffeic acid for selective growth of cryptococcus neoformans. | an agar medium containing inositol and urea as sole carbon and nitrogen sources, caffeic acid and ferric citrate as agents for the selective pigmentation of cryptococcus neoformans, gentamicin as a broad-spectrum bacterial antibiotic, and yeast nitrogen base without amino acids and ammonium sulfate (difco) was tested against 137 clinical isolates, 4 survey specimens, and 11 atcc yeast and yeast-like strains. all 28 strains of c. neoformans showed heavy growth and dark brown pigmentation after 36 ... | 1977 | 334795 |
| evaluation of a caffeic acid-ferric citrate test for rapid identification of cryptococcus neoformans. | an evaluation of a rapid caffeic acid-ferric citrate paper disk test for the identification of cryptococcus neoformans, using 474 isolates of yeasts and yeastlike organisms, showed that 96.6, 97.7, and 98.3% of 176 isolates of c. neoformans produced brown to dark-brown pigment on disks incubated for 6 h at room temperature, 30 degrees c, and 37 degrees c, respectively. all c. neoformans produced brown to dark-brown pigment within 24 h. however, nonspecific pigmentation was encountered at all tem ... | 1977 | 336639 |
| in vitro survival of human pathogenic fungi in seawater. | the survival of propagules from 4 pathogenic fungi, trichophyton mentagrophytes, trichosporon cutaneum, candida albicans, and microsporum gypseum was studied in seawater subjected to different temperature (20--35 degrees c) and salinity (6--50%) levels in diurnal rhythm of 12 h cycles. survival was measured by viability of propagules over a period of 52 weeks. all fungi, except t. cutaneum at 35 degrees c survived the experimental conditions for 52 weeks. temperature was the most influential fac ... | 1979 | 375437 |
| in vitro survival of human pathogenic fungi in hawaiian beach sand. | in vitro studies utilizing 4 pathogenic fungi, trichosporon cutaneum, candida albicans, microsporum gypseum and trichophyton mentagrophytes, all known from hawaiian beaches, indicate that they survive in the fluctuating beach habitat where they can serve as potential sources of infection for significant periods of time. survival was measured by testing the viability of propagules at intervals for 6 months. all species survived 6 months under 1 or more experimental conditions. survival patterns s ... | 1979 | 375438 |
| catabolism of l-tyrosine in trichosporon cutaneum. | protocatechuic acid was a catabolite in the degradation of l-tyrosine by trichosporon cutaneum. intact cells oxidized to completion various compounds proposed as intermediates in this conversion, but they did not readily oxidize catabolites of the homogentisate and homoprotocatechuate metabolic pathways, which are known to function in other organisms. cell extracts converted tyrosine first to 4-hydroxycinnamic acid and then to 4-hydroxybenzaldehyde and 4-hydroxybenzoic acid. the proposed hydrati ... | 1979 | 571434 |
| trichosporon cutaneum endocarditis. | a 61-year-old man with trichosporon cutaneum (t. cutaneum) prosthetic valve endocarditis is reported. he had had an aortic valve replacement for rheumatic heart disease 3 years earlier. onset of the valve infection was subacute. a systolic murmur was noted on admission. subsequently, he developed conjunctival hemorrhages, hematuria and transient episodes of confusion, aphasia and cranial nerve palsies. three of 17 blood cultures taken over 3 weeks were positive for t. cutaneum. he was given amph ... | 1978 | 715385 |
| [letter: yeasts isolated from human urinary sample. prevalence and high resistance of trichosporon cutaneum]. | | 1976 | 785383 |
| phenol hydroxylase from yeast. sulfhydryl groups in phenol hydroxylase from trichosporon cutaneum. | thiol groups in phenol hydroxylase were measured using two different -sh reagents and amino acid analysis. stepwise blocking of the -sh groups was correlated with enzyme activity and fad content. the results indicate that the enzyme contains 16 -sh groups per molecule of mr 1.48 x 10(5). at least four -sh groups are not accessible without the use of a denaturing agent. there is seemingly no disulphide bridge. on the whole, the reactivity towards p-hydroxymercuribenzoate is much greater than towa ... | 1975 | 810352 |
| mycoses of the sudan. | mycoses in the sudan are still significant and, as well as mycetoma, there are a number of fungal diseases: paranasal aspergillus granuloma, which came into eminence in 1966; actinomycosis; subcutaneous phycomycosis; systemic phycomycosis; post-operative opportunistic infection of the eye with trichosporon cutaneum and rhinosporidiosis. mycetoma and paranasal aspergillus granuloma are discussed in detail. | 1977 | 888164 |
| yeast-like fungi recovered from normal human skin in nsukka (nigeria). | the incidence of yeast-like fungi on normal skin was investigated in 52 healthy nsukka individuals comprising 21 adults, 24 children, and 7 infants. eighty-six isolates of yeasts and 2 of geotrichum candidum were recovered. the yeasts included candida tropicalis-36 isolates, c. guilliermondii-3, c. parapsilosis-2, candida spp. (unidentified)-16, cryptococcus albidus-6, trichosporon cutaneum-7, trichosporon spp.-8 and rhodotorula spp.-8 isolates. the isolates originated from various sites, predom ... | 1976 | 1085120 |
| [yeast flora of plant rhizosphere and phyllosphere]. | the species belonging to the genera cryptococcus and hansenula with saturnian spores predominate in the rhizosphere of agricultural plants. the sporiferous strains of debaryomyces, hanseniaspora apiculata, metschnikowia pulcherrima, and asporogenic candida krusei and trichosporon cutaneum prevail in the rhizosphere of wild plants. candida krusei and trichosporon cutaneum prevail in the rhizosphere of wild plants. the cultures of rhodotorula, candida krusei and metschnikowia pulcherrima are typic ... | 1975 | 1226148 |
| [effect of the chemical nature of the carbon source in the medium on the makeup of the biomass]. | the yeast candida tropicalis, candida utilis, trichosporon cutaneum were cultivated on the synthetic medium containing additions of group b vitamins (biotin, thiamin) and glucose, glycerol of lactic acid as the sole source. the protein content, amino acid composition, nucleic acids and complex b vitamins were identified in the resultant biomass. the carbon source in the medium affected these indices. the protein and nucleic acid content also depended on the yeast strain. | 1975 | 1237887 |
| [cultivation of the yeast trichosporon cutaneum k-1 on milk serum]. | yeast trichosporon cutaneoum k-1 were cultivated on milk serum supplemented with nitrogen and phosphorus sources. the culture showed a high biosynthetic capacity. the specific growth rate (mu) of yeast on milk serum was 0.40-0.45 hr-1. during lactose assimilation in the synthetic medium mu was 0.43 hr-1 and the economic coefficent of the biomass yield was 54%. it was shown that assimilation first involved lactose and then organic acids. the composition of the resulting biomass was identified. | 1975 | 1239760 |
| mycotic flora in the lower digestive tract of feral pigeons (columba livia) in the el paso, texas area. | fourteen species of fungus were isolated from the lower digestive tract of 39 of 80 pigeons. sixteen pigeons had concurrent isolations while two harbored three species. fungi isolated were allescheria boydii, aspergillus spp., candida krusei, chrysosporium spp., geotrichum candidum, mucor spp., paeciliomyces spp., penicillium spp., rhizopus spp., rhodotorula spp., scopulariopsis spp., streptomyces spp., and trichosporon cutaneum. there was no apparent evidence that these fungi were associated wi ... | 1976 | 1255918 |
| vaginal yeast flora of pregnant women in the cusco region of peru. | a study of the vaginal yeast flora in pregnant women living in cusco and in its region (peru), located approximately 3000 m above sea level, is reported. we observed 300 pregnant, healthy and non-diabetic women who attended a gynaecological clinic in the lorena, regional or ipss (instituto peruano de seguridad social) hospitals in cusco. a comprehensive clinical history was obtained from each patient. it included age, work, parity, time of pregnancy, use of contraceptives or antibiotics, type of ... | 1992 | 1291874 |
| effect of cigarette smoking on prevalence of summer-type hypersensitivity pneumonitis caused by trichosporon cutaneum. | we investigated the effect of cigarette smoking on the prevalence of summer-type hypersensitivity pneumonitis (shp) caused by trichosporon cutaneum. in the adult family members of shp patients, we found that 27 of 41 (65.9%) nonsmokers were shp patients, compared with 3 of 11 (27.3%) smokers (p less than .05). also, the prevalence of anti-t. cutaneum antibody was significantly lower in the smokers (p less than .05). a questionnaire provided to 209 shp patients revealed that the smoking rates of ... | 1992 | 1344064 |
| amperometric determination of ammonium ions with a microbial sensor. | a sensor for nh+4 ions has been developed, which consists of immobilized micro-organisms (bacillus subtilis, pseudomonas aeruginosa, trichosporon cutaneum) in combination with an electrochemical transducer. this sensor is based on the measurement of acceleration of respiration after addition of nh+4 in the presence of glucose. the physiological background of this signal and its connection with nh+4 ion uptake and/or metabolism is discussed. the response time of the sensor is about 5-10 s for nh+ ... | 1990 | 1367422 |
| phenol hydroxylase from trichosporon cutaneum: gene cloning, sequence analysis, and functional expression in escherichia coli. | a cdna clone encoding phenol hydroxylase from the soil yeast trichosporon cutaneum was isolated and characterized. the clone was identified by hybridization screening of a bacteriophage lambda zap-based cdna library with an oligonucleotide probe which corresponded to the n-terminal amino acid sequence of the purified enzyme. the cdna encodes a protein consisting of 664 amino acids. amino acid sequences of a number of peptides obtained by edman degradation of various cleavage products of the puri ... | 1992 | 1429434 |
| [clinical study of bronchial inhalation challenge in summer-type hypersensitivity pneumonitis induced by trichosporon cutaneum]. | to evaluate the methods and criteria of judgement for the bronchial antigen inhalation challenge test, the test was performed with culture filtrate antigen of serotype i and ii of trichosporon cutaneum in 18 patients with summer-type hypersensitivity pneumonitis from 15 families. the quantity of 15 mg of culture filtrate antigen was adequate, and had no side effects. in the tests, 17 of 18 patients showed a positive reaction to both or either, serotype of antigen. in 36 performances of inhalatio ... | 1992 | 1484431 |
| generation of genetic recombinants in trichosporon cutaneum by spontaneous segregation of protoplast fusants. | auxotrophic mutants were isolated in two strains of trichosporon cutaneum. complementing auxotrophs were hybridized by protoplast fusion. some of the fusants were apparently transient diploids and segregated to give recombinant marker combinations. | 1992 | 1512572 |
| orofacial fungal infections. nine pathogens that may invade during chemotherapy. | immunosuppression due to antineoplastic drugs or malignant tumor leaves patients extremely vulnerable to infection. opportunistic fungi that rarely infect healthy persons can have very severe consequences in these patients. candida albicans is the pathogen found most often, but several other candida species may cause infection as well. aspergillosis is the second most frequently seen fungal infection of the face and mouth in patients receiving chemotherapy. less commonly seen but equally dangero ... | 1992 | 1561171 |
| analysis of cases of central nervous system fungal infections reported in japan between january 1979 and june 1989. | data from 129 cases of central nervous systemic fungal infections reported in japan between january 1979 and june 1989 were analyzed. of 129 cases, 116 were cryptococcal meningitis, 6 candidal meningitis (including meningitis due to trichosporon cutaneum) and 7 aspergillus meningoencephalitis. fifty-six of the patients with cryptococcal meningitis had an underlying systemic disease or condition. the overall survival rate was 72.4%, which was a markedly improved rate compared to earlier reports. ... | 1992 | 1600262 |
| [in vitro antifungal activity of itraconazole, a new triazole antifungal agent, against clinical isolates from patients with systemic mycoses]. | the in vitro antifungal activity of itraconazole (itz), a new oral triazole antifungal agent, against clinical isolates from patients with systemic mycoses were compared with those of existing systemic antifungals, viz. ketoconazole (kcz), miconazole or amphotericin b. the studies were performed with 65 isolates of pathogenic yeasts and 13 isolates of aspergillus spp. using the agar dilution method on casitone agar. itz showed the most potent antifungal activities against isolates of pathogenic ... | 1991 | 1652654 |
| lowered responsiveness of bronchoalveolar lavage t lymphocytes in hypersensitivity pneumonitis. | we previously reported that trichosporon cutaneum was the major causative antigen of summer-type hypersensitivity pneumonitis (hp) in japan. in summer-type hp patients, we noticed that the proliferative responses of bronchoalveolar lavage (bal) lymphocytes to phytohemagglutinin (pha) and concanavalin a were significantly lower than those of the peripheral blood lymphocytes of the same patients. it was shown in this study that the low response of bal lymphocytes was due to an intrinsic lowering o ... | 1991 | 1673617 |
| importance of serotype-related antigen in the induction of experimental hypersensitivity pneumonitis by trichosporon cutaneum in rabbits. correlation between granulomatous alveolitis and cellular and humoral immune responses to polysaccharide-rich antigen. | we evaluated the immunopathogenic properties of trichosporon cutaneum, a major etiologic agent of japanese summer-type hypersensitivity pneumonitis (hp). when the culture filtrate antigen of t. cutaneum was chromatographed on deae-cellulose, two peaks of polysaccharide, fractions a and b, were obtained. fraction b was highly reactive to the specific igg and iga antibodies in the serum and bronchoalveolar lavage fluid samples obtained from sensitized rabbits, whereas fraction a was mainly reactiv ... | 1990 | 1689976 |
| [a case of severe summer-type hypersensitivity pneumonitis treated with high-dose administration of steroid]. | a 40-year-old man who lived in a wooden house built 30 years ago presented with complaints of fever, dry cough and dyspnea. chest x-ray findings showed interstitial shadows throughout bilateral lung fields. after admission, high-dose administration of 3000 mg of methylprednisolone was performed because of deterioration of chest x-ray shadows and symptoms. in a week, clinical data and symptoms improved. findings of bal fluid on admission revealed a relative increase of lymphocytes, neutrophils an ... | 1991 | 1770686 |
| genetic transformation of auxotrophic mutants of the filamentous yeast trichosporon cutaneum using homologous and heterologous marker genes. | a transformation system for the filamentous yeast trichosporon cutaneum based on auxotrophic markers is presented and techniques for the induction, isolation and characterization of mutants are described. a number of auxotrophic mutants were isolated and characterized by using biosynthetic precursors and/or inhibitors. a mutant unable to grow in the presence of ornithine could be complemented successfully by spheroplast transformation experiments using the cloned aspergillus nidulans ornithine t ... | 1991 | 1897316 |
| the n-terminal amino acid sequence of phenol hydroxylase contains a dinucleotide-binding sequence motif. | the n-terminal sequence of phenol hydroxylase from trichosporon cutaneum was determined by edman degradation of the integral protein and of fragments obtained by hydroxylamine cleavage and by digestion with staphylococcus v8 protease. a continuous sequence of 80 residues from the n terminus was determined: tkysesycdv10, livgagpagl20 maarvlseyv30 rqkpdlkvri40 idkrstkvyn50 gqadglqcrt60 leslknlrla70 dkixsexndm80. a single n-terminal sequence was detected, suggesting two identical subunits in the di ... | 1991 | 1924266 |
| japanese summer-type hypersensitivity pneumonitis. geographic distribution, home environment, and clinical characteristics of 621 cases. | a survey of hypersensitivity pneumonitis in japan was carried out by nationwide questionnaire. a total of 835 cases of hypersensitivity pneumonitis was recognized during the last decade from 1980 to 1989. of the 835 cases, 621 (74.4%) were summer-type hypersensitivity pneumonitis. the number of cases diagnosed doubled in the latter half of the 10-yr period. the residence of the patients was mostly in the western and southern parts of the country; the northernmost incidence was in akita prefectur ... | 1991 | 1928946 |
| fibroblast proliferation factors in pulmonary granuloma induced by trichosporon cutaneum in rabbits: presence of a lymphocyte-derived fibroblast proliferation factor and its functional specificity. | the fibroblast proliferation activity (fpa) in pulmonary granulomatous lesions in rabbits which were exposed once (primary response) or twice (secondary response) to trichosporon cutaneum was examined using r9ab, a rabbit fibroblast cell line cell, and fibroblasts from the lesions of the primary and secondary responses. the fpa in lung extracts and cell-free culture supernatants of bronchoalveolar lavage cells from the secondary response was more evident than that from the primary response. fpa ... | 1991 | 1959972 |
| purification and properties of two lactose hydrolases from trichosporon cutaneum. | two enzymes that hydrolysed lactose were purified essentially to homogeneity from cell extracts of the oleaginous yeast trichosporon cutaneum. one enzyme of mr 120,000 had properties typical of a beta-galactosidase (ec 3.2.1.23). it hydrolysed lactose, lactulose and nitrophenyl-beta-d-galactosides. the enzyme required k+ or rb+ for activity, and other monovalent cations tested were not effective. enzyme activity was abolished by edta and stimulated by mg2+, mn2+ and ca2+. the beta-galactosidase ... | 1990 | 2124610 |
| growth of opportunistic yeasts on vitamin-free solid medium. | the growth of some opportunistic yeasts (candida albicans, cryptococcus neoformans, torulopsis glabrata, rhodotorula rubra, geotrichum candidum, trichosporon cutaneum, saccharomyces cerevisiae) was essayed in a solid minimal synthetic medium without vitamins. from the preliminary results some species (candida albicans, cryptococcus neoformans, rhodotorula rubra, geotrichum candidum and saccharomyces cerevisiae) seem to be autotrophic regarding the growth factor considered indispensable for their ... | 1990 | 2191196 |
| [lung injuries and serial bronchoalveolar lavage findings in patients with summer-type hypersensitivity pneumonitis]. | to evaluate acute lung injuries and their persistence in patients with summer-type hypersensitivity pneumonitis, repeated bronchoalveolar lavage (bal) and pulmonary function tests were performed. bal was performed on 36 occasions in 17 patients with hypersensitivity pneumonitis. nineteen bal procedures in 16 cases were done during the active phase within the three hospital day and bal was repeated in 8 cases during inactive phase. anti-trichosporon cutaneum antibodies were detected in all of 15 ... | 1990 | 2214414 |
| transfer and expression of heterologous genes in yeasts other than saccharomyces cerevisiae. | in the past few years, yeasts other than those belonging to the genus saccharomyces have become increasingly important for industrial applications. species such as pichia pastoris, hansenula polymorpha, schizosaccharomyces pombe, yarrowia lipolytica and kluyveromyces lactis have been modified genetically and used for the production of heterologous proteins. for a number of additional yeasts such as schwanniomyces occidentalis, zygosaccharomyces rouxii, trichosporon cutaneum, pachysolen tannophil ... | 1990 | 2291442 |
| serotype-related antigen of trichosporon cutaneum in the induction of summer-type hypersensitivity pneumonitis: correlation between serotype of inhalation challenge-positive antigen and that of the isolates from patients' homes. | inhalation challenge with the culture filtrate-antigens prepared from two strains of different serotype of trichosporon cutaneum (timm 1573, serotype i; timm 1318, serotype ii) was performed on patients with summer-type hypersensitivity pneumonitis and asymptomatic seropositive family members. of the 17 patients, 12 were strongly positive, four were mildly so, and one was negative. interestingly, of the 16 inhalation challenge-positive patients, four reacted to both serotypes i and ii, five to s ... | 1990 | 2299104 |
| yeasts and fungi occurring in ensiled whole-crop maize and other ensiled vegetable crops. | the yeast flora of whole-crop maize ensiled for two weeks was predominated by candida holmii, c. lambica, c. milleri, hansenula anomala and saccharomyces dairensis. inoculation with other yeast species reported in the literature to prevail in maize or wheat silages did not alter the yeast flora. at 25 or 30 degrees c the ascomycetous fermentative species found at 20 degrees c were accompanied with ascomycetous non-fermentative fungi, i.c. exophiala jeanselmei and verticillium psalliotae, by the ... | 1990 | 2321936 |
| first report of chronic meningitis caused by trichosporon beigelii. | trichosporon beigelii (trichosporon cutaneum) was identified as the causative agent of chronic meningitis in a 15-year-old boy with acute lymphocytic leukaemia. after a neutropenic episode following cytostatic treatment and itraconazole therapy as prophylaxis, cerebrospinal fluid (csf) samples yielded growth of trichosporon beigelii. treatment with amphotericin b, flucytosine and high doses of fluconazole was followed by clinical improvement, although csf pleocytosis remained. the cross-reactivi ... | 1990 | 2338090 |
| survey of keratinophilic and saprobic fungi in the cloven-hooves and horns of goats and sheep from egypt. | seventy-six species belonging to 29 genera were collected from 320 samples of cloven-hooves and horns of goats and sheep. chrysosporium was the most common genus on the various substrates and comprehended the following species: c. asperatum, chrysosporium anamorph of arthroderma cuniculi, chrysosporium anamorph of arthroderma curreyi, c. georgii, c. indicum, c. keratinophilum, c. lobatum, c. lucknowense, c. pannicola, c. pseudomerdarium, c. queenslandicum, chrysosporium state of thielavia sepedo ... | 1990 | 2338621 |
| [in vitro activity of fluconazole, a novel bistriazole antifungal agent]. | fluconazole is a novel triazole antifungal agent with excellent activities against a broad range of medically important fungi. the in vitro antifungal activities of fluconazole especially against candida albicans were examined and the results summarized as follows: 1. fluconazole was proved to exhibit the highest antifungal activity in synthetic amino-acid medium, fungal (saamf), well buffered in a physiologically neutral range. 2. in a exposed time-killing test performed using saamf (ph 7.4), t ... | 1989 | 2540356 |
| fluconazole therapy for fungal peritonitis in continuous ambulatory peritoneal dialysis (capd): a case report. | fluconazole proved effective in treating fungal peritonitis caused by trichosporon cutaneum. fluconazole seems to offer several advantages over other antifungal drugs in the treatment of fungal peritonitis in continuous ambulatory peritoneal dialysis (capd) patients. | 1989 | 2562175 |
| [deep candida and related yeast infections]. | yeast-like fungi that are commonly found on the skin and in the digestive tract of healthy people may be responsible for deep infections as well as for cutaneous and mucosal diseases. such deep infections have long been known in the case of candida spp.; they are facilitated by immunosuppression or by factors that enable this opportunistic organism to proliferate in the gut, skin or mucosae and to penetrate into deep tissues. in addition to such well-known clinical manifestations as septicaemia ... | 1989 | 2682988 |
| genetic transformation of the filamentous yeast, trichosporon cutaneum, using dominant selection markers. | an efficient transformation system for the filamentous yeast, trichosporon cutaneum, has been developed. transformation was obtained with plasmids carrying either the escherichia coli hygromycin b phosphotransferase-encoding gene (hph) or the streptoalloteichus hindustanus phleomycin-resistance gene (ble), as dominant selection markers. expression of both resistance-conferring genes was controlled by the gpd promoter and the trpc terminator, from aspergillus nidulans. the transformation frequenc ... | 1989 | 2693213 |
| simultaneous utilization of phenol and other carbon sources during continuous culture of trichosporon cutaneum. | | 1989 | 2750313 |
| regulation of oxidation-reduction potentials of anthranilate hydroxylase from trichosporon cutaneum by substrate and effector binding. | the ph dependence of the redox behavior of anthranilate hydroxylase from trichosporon cutaneum in its uncomplexed and anthranilate-complexed forms, as well as the effects on the reduction potential, at ph 7.4, of enzyme in complex with 3-methylanthranilate, salicylate, 3-acetylpyridine adenine dinucleotide phosphates, and azide plus anthranilate, is described. at ph 7.4 the midpoint potential of uncomplexed enzyme (eflox/eflredh-) is -0.229 v vs she, close to that of free flavin. the aromatic su ... | 1989 | 2765477 |
| a rapid reaction study of anthranilate hydroxylase. evidence for a catalytically important conformational change during slow initial turnover with anthranilate. | rapid reaction kinetics of the flavoprotein anthranilate hydroxylase from trichosporon cutaneum were examined for reactions involving anthranilate, the native substrate. as was reported earlier for the nonhydroxylated substrate analogue, salicylate, some reactions in the first turnover with anthranilate occur slower than those in subsequent turnovers (powlowski, j., massey, v., and ballou, d. p. (1989) j. biol. chem. 264, 5606-5612). evidence is presented for slow conformational changes that occ ... | 1989 | 2777775 |
| hla-dqw3 in japanese summer-type hypersensitivity pneumonitis induced by trichosporon cutaneum. | the phenotype of the major histocompatibility complex, loci a, b, c, dr, and dq, was studied in 66 patients with japanese summer-type hypersensitivity pneumonitis induced by trichosporon cutaneum, and the results were compared with those obtained from 472 normal healthy subjects. our results showed an increase in the frequency of hla-dqw3 antigen in the patients (66% compared with 49% in the normal healthy control subjects: chi 2 = 5.48, p = 0.018, rr = 1.92), suggesting the importance of the ho ... | 1989 | 2802380 |
| prevention of summer-type hypersensitivity pneumonitis: effect of elimination of trichosporon cutaneum from the patients' homes. | to prevent summer-type hypersensitivity pneumonitis, the effect of eliminating trichosporon cutaneum from patients' homes was investigated. to determine environmental factors associated with enhanced indoor mold growth, we compared 11 patients' homes in which t. cutaneum was isolated to 32 control homes by inspection and indoor mold survey. statistically significantly higher isolates of t. cutaneum, total yeasts, and total fungi were associated with unsanitary rooms, and especially with damp pla ... | 1989 | 2817962 |
| a proton-translocating adenosine triphosphatase is associated with the peroxisomal membrane of yeasts. | the association of an atpase with the yeast peroxisomal membrane was established by both biochemical and cytochemical procedures. peroxisomes were purified from protoplast homogenates of the methanol-grown yeast hansenula polymorpha by differential and sucrose gradient centrifugation. biochemical analysis revealed that atpase activity was associated with the peroxisomal peak fractions which were identified on the basis of alcohol oxidase and catalase activity. the properties of this atpase close ... | 1987 | 2883951 |
| uptake of phenol by trichosporon cutaneum. | the soil yeast trichosporon cutaneum, which is distinguished by having a strictly oxidative metabolism, can be induced to utilize phenol as a sole carbon source. the present paper shows that such phenol-induced cells contain a specific, energy-dependent uptake system for phenol. phenol uptake is not directly linked to its o-hydroxylation inside the cell, the first step of phenol metabolism. the km for uptake is 235 +/- 30 microm, that for hydroxylation only 4.5 +/- 0.5 microm. further, the pheno ... | 1985 | 2981818 |
| [analysis of trichosporon cutaneum and cryptococcus neoformans antigens by crossed immunoelectrophoresis using antiserum, sera and bronchoalveolar lavage fluid obtained from patients with summer-type hypersensitivity pneumonitis]. | | 1988 | 3074210 |
| different classes of antibody activities to trichosporon cutaneum antigen in summer-type hypersensitivity pneumonitis by enzyme-linked immunosorbent assay. | to assess the diagnostic value of antibody activities to trichosporon cutaneum antigen in patients with summer-type hypersensitivity pneumonitis in japan, we measured the serum igg, iga, and igm antibodies to saline-soluble antigen of homogenized t. cutaneum cells by enzyme-linked immunosorbent assay (elisa). the igg antibody activity was observed in serum samples from 25 (83%) of the 30 patients and in 9 (82%) of the 11 asymptomatic family members. the iga antibody activity was found in 25 (83% ... | 1986 | 3079979 |
| experimental hypersensitivity pneumonitis in rabbits induced by trichosporon cutaneum: role of local cellular and humoral immune responses. | we evaluated the immunopathologic properties of trichosporon cutaneum, an important etiologic agent of summer-type hypersensitivity pneumonitis in japan. rabbits were sensitized and challenged by either a culture-filtrate antigen or a particulate antigen prepared from a timm 1318 strain of the fungus. group 1 animals received repetitive intratracheal injections of a particulate t. cutaneum antigen without use of any adjuvant. group 2 animals were sensitized by foot-pad injections of particulate ... | 1988 | 3172178 |
| activation enthalpies and ph dependence of phenol hydroxylase from trichosporon cutaneum, in vitro and in situ. | the effect of ph and temperature on phenol hydroxylase in vitro was compared to the corresponding effect on the enzyme in situ, in permeabilized cells. activation enthalpies in situ were about 75-80% of those in vitro, in both cases decreasing with increasing ph (6.0-8.5). the order of addition of phenol and nadph affected the km values for phenol at 25 degrees c, but not at 10 degrees c. the results support the idea that the enzyme in situ is in a more favourable position for catalysis than the ... | 1988 | 3203745 |
| enzyme activities in oleaginous yeasts accumulating and utilizing exogenous or endogenous lipids. | the activities of atp: citrate lyase (acl; ec 4. 1. 3. 8), carnitine acetyltransferase (cat; ec 2. 3. 1. 7), nadp+-dependent isocitrate dehydrogenase (icdh; ec 1. 1. 1. 42), isocitrate lyase (icl; ec 4. 1. 3. 1) and malic enzyme (malate dehydrogenase; ec 1. 1. 1. 40) were measured in four oleaginous yeasts, candida curvata d, trichosporon cutaneum and two strains of rhodosporidium toruloides, grown either to accumulate lipid, or to utilize their own lipid reserves or an exogenous supply of lipid ... | 1988 | 3254939 |
| c1q and c3 in bronchoalveolar lavage fluid from patients with summer-type hypersensitivity pneumonitis. | immune complexes have been thought to participate in the pathogenesis of hypersensitivity pneumonitis, but the role of complement components is not defined. in our study of nine patients with summer-type hypersensitivity pneumonitis (summer-type hp), c1q in bronchoalveolar lavage fluid (balf) was strikingly increased (mean 3.7, range 0.4 to 10 micrograms/ml). the value of c1q/albumin was several to 20 times greater in balf than in serum samples from individual patients. in contrast, balf samples ... | 1988 | 3257185 |
| allergenic cross-reactivity of yeasts. | yeast allergen extracts of candida albicans, c. pseudotropicalis, c. krusei, c. parapsilosis, c. tropicalis, c. guilliermondi, c. humicola, c. norwegica, c. utilis, cryptococcus albidus, geotrichum candidum, pityrosporon pachydermatis, p. ovale, rhodotorula minuta, r. rubra, saccharomyces cerevisiae, torulopsis glabrata and trichosporon cutaneum were investigated regarding their common allergenic properties. the enzyme immunoassay (eia) using rabbit anti-candida albicans antiserum showed remarka ... | 1988 | 3287997 |
| invasive trichosporon cutaneum (beigelii) infection in a patient with relapsed acute myeloid leukemia undergoing bone marrow transplantation. | | 1988 | 3293275 |
| surgical treatment of a deep fungal infection of the skin by trichosporon cutaneum. | | 1987 | 3301955 |
| invasive trichosporon cutaneum infection: an increasing problem in immunosuppressed patients. | trichosporon cutaneum (syn. t. beigelii), a saprophytic fungal organism normally found in the soil, is increasingly being recognized as a cause of life-threatening systemic illness in immunosuppressed patients. of 42 cases reported in the literature, 27 (64%) have died, including all four occurring after bone marrow transplantation. we report here a 44-year-old man who developed an invasive pulmonary infection and fungemia with t. cutaneum following bone marrow transplantation. the infection was ... | 1987 | 3332179 |
| induction of high-affinity phenol uptake in glycerol-grown trichosporon cutaneum. | two uptake systems for phenol are identified in trichosporon cutaneum. one is an inducible, high-affinity system, sensitive to protonophores. it is induced coordinately with phenol hydroxylase but can operate independently of phenol metabolism. the other is a constitutive, low-affinity system with different specificity and different ph optimum. it is not sensitive to protonophores. | 1988 | 3360747 |
| [summer-type hypersensitivity pneumonitis induced by trichosporon cutaneum in terms of the role of serotype on the antigen]. | | 1988 | 3404781 |
| trichosporon cutaneum c.a.p.d. peritonitis. | | 1987 | 3451213 |
| systemic infections with trichosporon beigelii (cutaneum). report of three new cases. | three new cases of systemic mycosis due to trichosporon cutaneum are reported and compared with the 23 previous reports. two patients had acute leukemia and one patient had a lymphoblastic lymphoma. blood cultures in two patients and cerebrospinal fluid in the third patient were positive for t. cutaneum. only one patient recovered after antimycotic therapy and concomitant remission of his leukemia. at autopsy, the two other patients showed widespread infection with t. cutaneum. the authors concl ... | 1986 | 3533246 |
| a 31p nmr study of the internal ph of yeast peroxisomes. | the internal ph of peroxisomes in the yeasts hansenula polymorpha, candida utilis and trichosporon cutaneum x4 was estimated by 31p nuclear magnetic resonance (nmr) spectroscopy. 31p nmr spectra of suspensions of intact cells of these yeasts, grown under conditions of extensive peroxisomal proliferation, displayed two prominent pi-peaks at different chemical shift positions. in control cells grown on glucose, which contain very few peroxisomes, only a single peak was observed. this latter peak, ... | 1987 | 3579461 |
| characterization of the amine oxidase involved in the growth of trichosporon cutaneum x4 on ethylamine as source of carbon, nitrogen and energy. | the amine oxidase from trichosporon cutaneum x4 grown on ethylamine as carbon, nitrogen and energy source was purified to near homogeneity. the purified enzyme showed the highest resistance to heat of any amine oxidase hitherto characterized from a yeast (half-life at 62 degrees c, 14 min). measurement of kinetic parameters as a function of carbon chain length showed results typical of a benzylamine oxidase. both non-denaturing- and sodium dodecyl sulphate-polyacrylamide gel electrophoresis show ... | 1987 | 3579462 |
| [anti-trichosporon cutaneum igg, iga and s-iga antibody activities in bronchoalveolar lavage fluids in summer-type hypersensitivity pneumonitis]. | | 1987 | 3599571 |
| specific bronchoalveolar lavage iga antibody in patients with summer-type hypersensitivity pneumonitis induced by trichosporon cutaneum. | patients with summer-type hypersensitivity pneumonitis induced by trichosporon cutaneum were studied with respect to specific igg, iga, and secretory iga (s-iga) antibody activities to t. cutaneum in serum and bronchoalveolar lavage fluid (balf). a strain of t. cutaneum was isolated from 1 patient's pillow. all serum samples from the family members (2 patients and 2 asymptomatic) contained igg antibody activity to t. cutaneum, and the titers showed no difference between the patients and asymptom ... | 1986 | 3729153 |
| facial granuloma associated with trichosporon cutaneum infection. | a 23-year-old woman presented with trichosporon cutaneum, which invaded deep into the dermis and subcutaneous tissue of the face and developed chronic granulomatous reactions. we postulated that the local use of an occlusive dressing with topical corticosteroid ointment in the early stage of the infection played a role in promoting the process of this opportunistic fungal infection. antifungal chemotherapies, selected according to sensitivity testing to the isolated organism, were unsuccessful, ... | 1986 | 3767402 |
| ascorbic acid specific utilization by some yeasts. | one hundred and eighty strains of yeasts belonging to 17 genus and 53 species were screened for their ability to grow on ascorbic acid and iso-ascorbic acid as the sole carbon source. most of the tested strains (157) were unable to grow on either compound. strains of seven species of the genus cryptococcus, of two candida species, of filobasidiella neoformans, trichosporon cutaneum, lipomyces starkeyi, hansenula capsulata, and one strain of aureobasidium pullulans were able to grow on ascorbic a ... | 1986 | 3779527 |
| transport and hydrolysis of disaccharides by trichosporon cutaneum. | trichosporon cutaneum is shown to utilize six disaccharides, cellobiose, maltose, lactose, sucrose, melibiose, and trehalose. t. cutaneum can thus be counted with the rather restricted group of yeasts (11 to 12% of all investigated) which can utilize lactose and melibiose. the half-saturation constants for uptake were 10 +/- 3 mm sucrose or lactose and 5 +/- 1 mm maltose, which is of the same order of magnitude as those reported for saccharomyces cerevisiae. our results indicate that maltose sha ... | 1986 | 3782022 |
| properties of anthranilate hydroxylase (deaminating), a flavoprotein from trichosporon cutaneum. | anthranilate hydroxylase was purified from the yeast trichosporon cutaneum. this enzyme is a simple flavoprotein which apparently does not require any additional cofactor for the conversion of anthranilate to 2,3-dihydroxybenzoate. anthranilate hydroxylase has mr of approximately 95,000, with subunit mr of 50,000; it contains 2 mol of fad/mol of enzyme. a number of compounds in addition to anthranilate serve as substrates, or effectors, for this enzyme. oxygen-labeling experiments show that the ... | 1987 | 3793735 |
| oxidation of amines by yeasts grown on 1-aminoalkanes or putrescine as the sole source of carbon, nitrogen and energy. | the maximum growth rate of trichosporon cutaneum cbs 8111 in chemostat cultures was 0.185 h-1 on ethylamine and 0.21 h-1 on butylamine, that of candida famata cbs 8109 was 0.32 h-1 on putrescine. the amine oxidation pattern of the ascomycetous strains studied, viz. candida famata cbs 8109, stephanoascus ciferrii cbs 4856 and trichosporon adeninovorans cbs 8244 was independent of the amine that had been used as the growth substrate. it resembled that of benzylamine/putrescine oxidase found in oth ... | 1986 | 3813525 |
| [study on the presence of yeasts in the feces of the rock pigeon (columba livia gmelin 1789) from rural areas]. | the diffusion of rock-pigeon (domestic form of columba livia gmelin 1789) is greatly increasing owing to its high reproductivity; for this reason it is present in both countries and cities. this bird is considered an important vector of pathogens to man and domestic animals, and today it represents a serious hygienic-sanitary problem. therefore we checked for the presence of yeasts in feces of rock-pigeons nesting in the regional park "la mandria" (near turin), visited mostly by school-children ... | 1985 | 3870648 |
| evaluation of the new mycotube test-kit for yeast identification. | the performance of a modified mycotube test for the identification of yeasts was evaluated using standard biochemical tests as reference. one hundred and eighty strains belonging to 12 medically important species (15 strains each) were tested. the overall rate of identification was 72%, and rose to 87.8% when macroscopic and microscopic morphologic features were also considered; a 100% rate was obtained with candida albicans, candida krusei, candida parapsilosis, candida pseudotropicalis, candid ... | 1985 | 3886377 |
| biogenesis and metabolic significance of microbodies in urate-utilizing yeasts. | growth of candida famata and trichosporon cutaneum on uric acid as the sole source of carbon and nitrogen was associated with the development of a number of microbodies in the cells. cytochemical staining experiments showed that the organelles contained urate oxidase, a key enzyme of uric acid metabolism, and catalase. transfer of cells, precultured on glucose or glycerol, into uric acid-containing media indicated that these microbodies originated from the organelles, originally present in the i ... | 1985 | 4004201 |
| [biotin transport in the yeast trichosporon cutaneum]. | biotin transport was studied in the yeast trichosporon cutaneum and was shown to be the active transport with km = 1.5 x 10(-7) m. the process depended on the ph and temperature whose optimal values were ph 6.8-7.2 and 35-40 degrees c. t. cutaneum biotin permease was highly specific for biotin and was inhibited by biotin analogs. biotin uptake by the cells was inhibited by glucose. excess biotin in the growth medium caused partial repression of its transport system in the yeast. synthesis of bio ... | 1985 | 4040203 |
| beta-ketoadipate pathway in trichosporon cutaneum modified for methyl-substituted metabolites. | trichosporon cutaneum, when grown with p-cresol, catalyzed intradiol fission of the benzene nucleus of 4-methylcatechol before the complete catabolism of these two substrates. steps in their conversion to pyruvate and acetyl coenzyme a were investigated by using cell extracts, and some properties of various new microbial catabolites are also described. these included (-)-2,5-dihydro-3-methyl-5-oxofuran-2-acetic acid (beta-methylmuconolactone) and (-)-3-keto-4-methyladipic acid and its coenzyme a ... | 1985 | 4040904 |
| enzymology of the beta-ketoadipate pathway in trichosporon cutaneum. | cell extracts were prepared from trichosporon cutaneum grown with phenol or p-cresol, and activities were assayed for enzymes catalyzing conversion of these two carbon sources into 3-ketoadipate (beta-ketoadipate) and 3-keto-4-methyladipate, respectively. when activities of each enzyme were expressed as a ratio, the rate for methyl-substituted substrate being divided by that for the unsubstituted substrate, it was apparent that p-cresol-grown cells elaborated pairs of enzymes for hydroxylation, ... | 1985 | 4040905 |
| association of anurans with pathogenic fungi. | in a study of 450 amazonian anurans, we isolated yeasts and yeast-like fungi from 54 animals (bufo granulosus, b. marinus, dendrophrynyscus sp., hyla geographica, h. lanciformes, ololygon rubra, adenomera hylaedactyla, eleutherodactylus fenestratus, leptodactylus fuscus, l. ocellatus, l. pentadactylus). the internal organs of these animals did not show any macroscopic anomaly nor histopathology. we recovered 105 fungal isolates from the anuran liver, lung, kidney, spleen, heart and gonad. the is ... | 1985 | 4069190 |
| yeast species utilizing uric acid, adenine, n-alkylamines or diamines as sole source of carbon and energy. | yeast strains utilizing uric acid, adenine, monoamines or diamines as sole source of carbon and energy were isolated from several soil samples by the enrichment culture method. the most common species was trichosporon cutaneum. strains of candida catenulata, c. famata, c. parapsilosis, c. rugosa, cryptococcus laurentii, stephanoascus ciferrii and tr. adeninovorans were also isolated. all strains utilizing uric acid as sole carbon source utilized some primary n-alkyl-l-amines, hydroxyamines or di ... | 1985 | 4091535 |
| septal pores in trichosporon cutaneum. | | 1971 | 4101787 |
| phenol hydroxylase from yeast. purification and properties of the enzyme from trichosporon cutaneum. | | 1973 | 4146224 |
| dark-field electron microscopy of thin sections of trichosporon cutaneum. | dark-field electron micrographs of aldehyde-fixed, unstained yeast-phase cells of trichosporon cutaneum exhibit remarkable contrast and good resolution when the sections studied are less than 25 nm thick and conical illumination is used. | 1974 | 4213592 |
| degradation of phenols by intact cells and cell-free preparations of trichosporon cutaneum. | | 1970 | 4392441 |
| postoperative endophthalmitis due to trichosporon cutaneum. | | 1974 | 4425639 |
| [trichosporon cutaneum bd-2 as a producer of fodder protein]. | | 1973 | 4797857 |
| the effect of 1,2-naphthoquinone on catechol 1,2-oxygenase from trichosporon cutaneum. | | 1972 | 5035953 |
| [demonstration of trichosporon cutaneum (de beurm., gougerot et vaucher) ota in the respiratory and digestive tract in miners with silicosis]. | | 1971 | 5106356 |
| [study of candida krusei and trichosporon cutaneum isolated from pozol]. | | 1971 | 5291974 |
| brain abscess due to trichosporon cutaneum. | | 1970 | 5448879 |
| purification and properties of catechol 1,2-oxygenase from trichosporon cutaneum. | | 1970 | 5462291 |
| a note on chlamydospore formation in trichosporon cutaneum. | | 1970 | 5531233 |
| cytology of trichosporon cutaneum and the effect of some antifungal agents on it. | | 1965 | 5839782 |
| prosthetic valve endocarditis caused by trichosporon cutaneum. | we report, to our knowledge, the first case of trichosporon cutaneum endocarditis which developed in a 58-year-old woman 14 months after mitral valve replacement. the patient had not had antibiotic therapy. she was treated with an oral antifungal agent, ketoconazol, and prosthetic valve replacement. the initial outcome was favorable, but she died 5 months later of aortic valve endocarditis. fungal infection was documented by isolation of the fungus from blood cultures, by anatomical and patholog ... | 1984 | 6319306 |