| cellulase formation by soil yeasts of the genus trichosporon and by microscopic fungi. | the production of celulases by a soil yeast belonging to the genus trichosporon and by microscopic fungi of various systematic groups was studied. the yeast synthesized relatively small amounts of cellulases in contrast to microscopic fungi. the active culture producing cellulases was the fungus trichoderma longibrachiatum 7-26. | 1978 | 26853 |
| the 1,4-beta-d-glucan glucanohydrolases from phanerochaete chrysosporium. re-assessment of their significance in cellulose degradation mechanisms. | a physico-chemical, functional and structural characterization, including partial sequence data, of three major 1,4-beta-d-glucan glucanohydrolases (ec. 3.2.1.4) isolated from the culture filtrate of the white-rot fungus phanerochaete chrysosporium, shows that all three enzymes belong to a single family of cellulases. eg44, pi 4.3, (named after its apparent molecular mass in kda), shows a clear homology with schizopyllum commune endoglucanase i (egi); whereas eg38, pi 4.9, (named in the same man ... | 1991 | 1367687 |
| cloning, sequence analysis and yeast expression of the egl1 gene from trichoderma longibrachiatum. | a gene (egl1) encoding an endoglucanase (egl1) from trichoderma longibrachiatum has been cloned and sequenced. this gene, homologous to the t. reesei egl1 gene, differs from it in the length of the introns (particularly the first one) and encoded protein. a cdna fragment obtained by the rapid amplification of cdna ends method, which takes advantage of the polymerase chain reaction, has been expressed in yeast under control of the cyc-gal inducible promoter and yeast clones able to secrete active ... | 1992 | 1369161 |
| antagonistic potential of gliocladium virens and trichoderma longibrachiatum to phytopathogenic fungi. | three isolates of gliocladium virens (g1, g2 and g3) and two of trichoderma longibrachiatum (t1 and t2) were screened against isolates of three soilborne plant pathogens namely rhizoctonia solani, sclerotium rolfsii and pythium aphanidermatum. g. virens exhibited stronger hyperparasitism and wider biological spectrum than t. longibrachiatum. further, similarities as well as variation was observed in the ability of the various isolates to invade the test pathogens in dual culture. for the hyperpa ... | 1990 | 2325746 |
| beta-glucosidases from cellulolytic fungi aspergillus terreus, geotrichum candidum, and trichoderma longibrachiatum as typical glycosidases. | by ethanol precipitation (v/v) and chromatography on sephadex sp, deae (or deae-cellulose), and g-200 beta-glucosidases (ec 3.2.1.21) from the culture filtrates of cellulolytic fungi aspergillus terreus, geotrichum candidum, and trichoderma longibrachiatum grown on the medium with cellulose containing materials were isolated. the enzymes were homogenous as shown by different techniques. the substrate specificities of the obtained enzymes were studied. beta-glucosidases had higher affinity for p- ... | 1987 | 3111495 |
| [simultaneous cultivation of the fungi trichoderma longibrachiatum and endomycopsis fibuligera]. | combined cultivation of the following microorganisms was studied: the fungus trichoderma longibrachiatum producing cellulases and the yeast endomycopsis fibuligera producing glucoamylase. a growth medium was found to maintain the activity of these enzymes at a high level in the both microbial monocultures. the effect of the yeast inoculation time on the enzyme activity was studied during combined cultivation of the two organisms. when the yeast was inoculated during the first two days of the fun ... | 1985 | 3925303 |
| metabolic disposition in rats of regular and enzymatically depolymerized sodium carboxymethylcellulose. | partially enzyme-hydrolysed sodium carboxymethylcellulose (cmc-enz), which holds promise as a new, functional food ingredient, is obtained from sodium carboxymethylcellulose (cmc) by enzymatic hydrolysis with a cellulase preparation from trichoderma longibrachiatum. in the safety evaluation of cmc-enz, a comparative disposition study on 14c-labelled cmc and cmc-enz was conducted in conventionally kept rats. the 14c label was in the two c atoms of the carboxymethyl group. two groups of four male ... | 1995 | 7590536 |
| development of a transformation system for trichoderma longibrachiatum and its use for constructing multicopy transformants for the egl1 gene. | an efficient transformation system for the fungus trichoderma longibrachiatum has been developed. transformation was obtained both by electroporation and polyethyleneglycol treatment, using a plasmid carrying the escherichia coli hygromycin b phosphotransferase gene as a dominant selectable marker. the transformation frequency was 0.5 to 5 transformants/micrograms plasmid dna. transformation normally occurred by tandem integration of the transforming dna. a high percentage of the transformants w ... | 1994 | 7765105 |
| rate of passage of barley diets with chromium oxide: influence of age and poultry strain and effect of beta-glucanase supplementation. | three-week-old broiler chicks and 1-yr-old leghorn cocks were used to determine the effect of beta-glucanase addition (experimental product from trichoderma longibrachiatum) to a 60% barley-based diet on rate of feed passage, transit time, and mean retention time. fecal chromium oxide content was used to calculate cumulated and noncumulated excretion data. a hill equation was chosen to test the goodness of fit of cumulative excretion curves. time of 50% (t50) and 1% (t1) chromium excretion, calc ... | 1994 | 7800645 |
| transcriptional regulation of the trichoderma longibrachiatum egl1 gene. | transcription of the trichoderma longibrachiatum egl1 gene is induced in the presence of lactose and beta-methylglucoside and repressed by glucose. a dna fragment containing 722 bp upstream of the atg codon has been sequenced. the gene has two major transcription start points (20 and 24 nucleotides upstream from the atg codon) and several transcription termination points (located in a region around 130 nt downstream of the stop codon). two 6-mer sequences (5'-ctggag-3') separated by 16 bp are pr ... | 1994 | 7988872 |
| an in vitro assay for prediction of broiler intestinal viscosity and growth when fed rye-based diets in the presence of exogenous enzymes. | a two-stage in vitro assay procedure was developed for predicting the efficacy of microbial enzyme sources in rye-based diets for broiler chicks. the procedure uses the complete diet as substrate to predict the in vivo intestinal viscosity and final weight of birds fed such diets. the optimum conditions for maximizing in vitro viscosity of a rye-based diet consisted of digesting .6 g of diet (ground through a 1-mm screen) in .9 ml of .1 n hcl containing 2,000 u pepsin/ml for 45 min at 40 c. at t ... | 1993 | 8426842 |
| molecular evidence that the asexual industrial fungus trichoderma reesei is a clonal derivative of the ascomycete hypocrea jecorina. | the relationship of the important cellulase producing asexual fungus trichoderma reesei to its putative teleomorphic (sexual) ancestor hypocrea jecorina and other species of the trichoderma sect. longibrachiatum was studied by pcr-fingerprinting and sequence analyses of the nuclear ribosomal dna region containing the internal transcribed spacers (its-1 and its-2) and the 5.8s rrna gene. the differences in the corresponding its sequences allowed a grouping of anamorphic (asexual) species of trich ... | 1996 | 8755548 |
| novel osmotically induced antifungal chitinases and bacterial expression of an active recombinant isoform. | nacl (428 mm)-adapted tobacco (nicotiana tabacum l. var wisconsin 38) cells accumulate and secrete several antifungal chitinases. the predominant protein secreted to the culture medium was a 29-kd peptide that, based on internal amino acid sequence, was determined to be a class ii acidic chitinase with similarity to pr-q. the four predominant chitinases (t1, t2, t3, and t4) that accumulated intracellularly in 428 mm nacl-adapted cells were purified. based on n-terminal sequence analyses, two of ... | 1996 | 8756502 |
| trichoderma longibrachiatum infection in a pediatric patient with aplastic anemia. | trichoderma longibrachiatum infection of the skin in an 11-year-old child with severe aplastic anemia and prolonged neutropenia is reported. the patient received systemic antifungal therapy and underwent bone marrow transplantation. to our knowledge, this is the first description of t. longibrachiatum infection in a pediatric patient. it also is the first case successfully treated with medical therapy. a review of the literature suggests that trichoderma spp. are recognized as human pathogens wi ... | 1997 | 9003627 |
| molecular reidentification of human pathogenic trichoderma isolates as trichoderma longibrachiatum and trichoderma citrinoviride. | several species of the well-known saprophytic genus trichoderma have been identified as the cause of infections in immunosuppressed humans. because the differentiation and identification of trichoderma species based on morphological characters only, is very difficult, two molecular approaches were applied for species identification. six human pathogenic trichoderma isolates were investigated by pcr-fingerprinting and analysis of ribosomal dna internal transcribed spacer (its) sequences and compa ... | 1999 | 10200931 |
| heterologous expression in aspergillus nidulans of a trichoderma longibrachiatum endoglucanase of enological relevance. | an aspergillus nidulans transformant expressing the trichoderma longibrachiatum endoglucanase 1 gene (egl1) has been constructed. the extracellular production of egl1 in different culture media has been studied, and a medium has been found in which egl1 is the predominant extracellular protein produced. the enzymatic properties of the heterologously produced egl1 are very similar to those of the native enzyme. grape maceration in the presence of culture filtrate enriched in egl1 resulted in incr ... | 2000 | 10725180 |
| relationships between activities of xylanases and xylan structures. | structures of five water-soluble xylans have been determined. four purified xylanase enzymes have been studied for the hydrolysis of the xylans. different xylanases have different activities against various xylan structures. the key factors that influence the rate of xylan hydrolysis are chain length and degree of substitution. two family 11 xylanases, orpinomyces pc2 xylanase and trichoderma longibrachiatum xylanase, can rapidly hydrolyze xylans that have a chain length greater than 8 xylose re ... | 2000 | 10862906 |
| synergy between ruminal fibrolytic enzymes and enzymes from trichoderma longibrachiatum. | the mechanism by which enzyme additives improve feed digestion in ruminants is not fully understood. direct hydrolysis of feed in the rumen is a potential mode of action, but the importance of this mode needs to be quantified because of the relatively low exogenous hydrolase activity added compared with the total activity present in the rumen. we examined the interactions between ruminal and exogenous enzymes on fiber degradation using a completely randomized experimental design, with an 11 (enz ... | 2000 | 10877396 |
| enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa. | we evaluated the effects of cellulase (from trichoderma longibrachiatum) application rates on neutral detergent fiber (ndf) concentration and fermentation products of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages harvested with decreasing dry matter (dm) digestibility. additionally, the impacts of inoculant (lactobacillus plantarum and pediococcus cerevisiae), pectinase (from aspergillus niger), or formic acid on silage composition were studied. forages wilted to ... | 2000 | 10908057 |
| intake, digestibility, and composition of orchardgrass and alfalfa silages treated with cellulase, inoculant, and formic acid fed to lambs. | the objectives of this study were to determine the effect of a cellulase (from trichoderma longibrachiatum) alone or combined with a bacterial inoculant (lactobacillus plantarum and pediococcus cerevisiae) or formic acid on composition, intake, and digestibility of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages. orchardgrass and alfalfa were harvested at the early heading stage and at the early bloom stage of maturity and wilted to approximately 22 and 32% dm, resp ... | 2000 | 11063325 |
| in vitro fungicidal activities of voriconazole, itraconazole, and amphotericin b against opportunistic moniliaceous and dematiaceous fungi. | the nccls proposed standard m38-p describes standard parameters for testing the fungistatic antifungal activities (mics) of established agents against filamentous fungi (molds); however, standard conditions are not available for testing their fungicidal activities (minimum fungicidal or lethal concentrations [mfcs]). this study evaluated the in vitro fungistatic and fungicidal activities of voriconazole, itraconazole, and amphotericin b against 260 common and emerging molds (174 aspergillus sp. ... | 2001 | 11230410 |
| comparison of the e-test with the nccls m38-p method for antifungal susceptibility testing of common and emerging pathogenic filamentous fungi. | the national committee for clinical laboratory standards (nccls) m38-p method describes standard parameters for testing the fungistatic antifungal activities (mics) of established agents against filamentous fungi (molds). the present study evaluated the in vitro fungistatic activities of itraconazole and amphotericin b by the e-test and the nccls m38-p microdilution method against 186 common and emerging pathogenic molds (123 isolates of aspergillus spp. [five species], 16 isolates of fusarium s ... | 2001 | 11283057 |
| resistance of feed enzymes to proteolytic inactivation by rumen microorganisms and gastrointestinal proteases. | potential feed enzyme additives for ruminants were tested in vitro for their stability to ruminal microbial and gastrointestinal proteolysis. four commercial preparations from trichoderma longibrachiatum (a, b, c, and d) and one from an undisclosed source (e) were incubated up to 6 h with ruminal fluid taken from four lactating dairy cows before or 2 h after feeding. the stability of preparation b was also tested in the presence of pepsin at ph 3 and pancreatin at ph 7. cellulase (ec 3.2.1.4), c ... | 2001 | 11424701 |
| sequences and antimycoplasmic properties of longibrachins lgb ii and lgb iii, two novel 20-residue peptaibols from trichoderma longibrachiatum. | longibrachins are members of the class of natural aib-containing peptides designated as peptaibols. six longibrachins, lga i-iv and lgb ii and iii, were purified from a trichoderma longibrachiatum strain by a procedure employing several chromatography steps including reversed-phase hplc. the amino acid sequence determination was based on a combination of liquid secondary ion mass spectrometry (lsims) and two-dimensional 1h and 13c nmr spectroscopy. longibrachins are 20-residue peptaibols with a ... | 2001 | 11429993 |
| salicylic acid and ethylene pathways are differentially activated in melon cotyledons by active or heat-denatured cellulase from trichoderma longibrachiatum. | infiltration of cellulase (ec 3.2.1.4) from trichoderma longibrachiatum into melon (cucumis melo) cotyledons induced several key defense mechanisms and hypersensitive reaction-like symptoms. an oxidative burst was observed 3 hours after treatment and was followed by activation of ethylene and salicylic acid (sa) signaling pathways leading to marked induction of peroxidase and chitinase activities. the treatment of cotyledons by heat-denatured cellulase also led to some induction of peroxidase an ... | 2001 | 11553761 |
| identification of trichoderma strains by image analysis of hplc chromatograms. | forty-four trichoderma strains from water-damaged building materials or indoor dust were classified with chromatographic image analysis on full chromatographic matrices obtained by high performance liquid chromatography with uv detection of culture extracts. the classes were compared with morphological identification and rdna sequence data, and for each class all strains were of the same identity. with all three techniques each strain--except one--was identified as the same species. these strain ... | 2001 | 11583856 |
| feed intake, growth, digestibility of dry matter and nitrogen in young pigs as affected by dietary cation-anion difference and supplementation of xylanase. | an experiment was conducted to test the effect of dietary cation-anion difference (cad, na(+) + k(+)-cl(-), meq/kg diet) and xylanase addition on feed consumption, digestibility of nutrients, plasma electrolyte balance and growth performance in young pigs. a 2 x 3 factorial arrangement with three dietary cad levels (-100, 200, and 500 meq/kg) and two levels of xylanase supplementation (0 and 0.1% xylanase derived from trichoderma longibrachiatum) was used. thirty-six individually housed, castrat ... | 2001 | 11686778 |
| effect of macerating enzymes on red wine aroma at laboratory scale: exogenous addition or expression by transgenic wine yeasts. | the effects of a trichoderma longibrachiatum endoglucanase and an aspergillus nidulans endoxylanase on the concentration of free and bound volatiles, color, and phenolics during maceration in red wine vinification has been studied. two different approaches have been considered for the utilization of these enzymes: (i) direct addition of the enzymes to must and (ii) inoculation of must with recombinant wine yeasts overexpressing these activities. an experimental design based on a taguchi orthogon ... | 2001 | 11714353 |
| antimicrobial activity of ergokonin a from trichoderma longibrachiatum. | natural fungal products were screened for antifungal compounds. the mode of action of one of the hits found and the taxonomy of the producing organism were analysed. | 2001 | 11722657 |
| effect of a fibrolytic enzyme preparation from trichoderma longibrachiatum on the rumen microbial population of dairy cows. | the effects of supplementing a dairy cow diet with incremental levels of a fibrolytic enzyme preparation (preparation b) from trichoderma longibrachiatum on the rumen microbial population were investigated. two cows fitted with rumen cannulae were each fed a diet containing barley-based concentrate (52%), maize silage (29%), and chopped alfalfa hay (19%), supplemented with 0, 1, 2, 5, or 10 l of preparation b per tonne of dry matter (dm). preparation b stimulated numbers of total viable bacteria ... | 2002 | 11888159 |
| effect of enzyme supplementation of a rye-based diet on xylanase activity in the small intestine of broilers, on intestinal crypt cell proliferation and on nutrient digestibility and growth performance of the birds. | 1. a study was undertaken to investigate the susceptibility to peptic digestion of exogenous xylanase (ec 3.2.1.8) from trichoderma longibrachiatum, added to a rye-based diet for broiler chickens, in order to elucidate its possible site of action. 2. it was also designed to investigate the effects of the enzyme (plus exogenous protease ec 3.4-24.28) when added to a rye-containing diet (60% rye/kg diet) on crypt cell proliferation in the mucosa of the small intestine, on short chain fatty acid (s ... | 2002 | 12047093 |
| optimal testing conditions for determining mics and minimum fungicidal concentrations of new and established antifungal agents for uncommon molds: nccls collaborative study. | this collaborative three-center study evaluated nccls m38-a document testing conditions and other testing conditions for the antifungal susceptibility testing of 35 isolates of aspergillus nidulans, a. terreus, bipolaris hawaiiensis, b. spicifera, cladophialophora bantiana, dactylaria constricta, fusarium solani, paecilomyces lilacinus, scedosporium prolificans, trichoderma longibrachiatum, and wangiella dermatitidis for itraconazole, three new triazoles (voriconazole, posaconazole, and ravucona ... | 2002 | 12354880 |
| a screening method for endo-beta-1,4-xylanase substrate selectivity. | endoxylanase (ec 3.2.1.8) substrate selectivity, i.e., its relative activity toward water-unextractable arabinoxylan (wu-ax) and water-extractable arabinoxylan (we-ax) substrates, is important for its functionality in biotechnological processes such as bread-making and gluten starch separation. a screening method for rapidly determining said substrate selectivity was developed. endoxylanase activity toward wu-ax was estimated by incubation of insoluble chromogenic substrate with a range of enzym ... | 2003 | 12842109 |
| allergic fungal sinusitis associated with trichoderma longibrachiatum. | we describe allergic fungal sinusitis caused by trichoderma longibrachiatum in a patient with a history of atopy and asthma. a gram stain of a sinus biopsy specimen was initially thought to contain yeast cells, but when trichoderma was recovered in culture, these cells were subsequently recognized as chlamydospores. the patient was successfully managed with a combination of sinus lavage, oral corticosteroids, itraconazole, and allergen immunotherapy. this case also points out that careful scruti ... | 2003 | 14605198 |
| cork workers' occupational asthma: lack of association with allergic sensitisation to fungi of the work environment. | to evaluate allergic sensitisation to chrysonilia sitophila, penicillium glabrum, and trichoderma longibrachiatum in cork workers with asthma. | 2004 | 14740220 |
| [germination of phytopathogenic fungi conidia]. | the autoregulation of conidium germination in phytopathogenic micromycetes of the genera fusarium, botrytis, and bipolaris was studied. it was shown that trichoderma longibrachiatum was less competitive than fusarium oxysporum after their simultaneous inoculation but inhibited the phytopathogen growth in the case of earlier introduction. in the latter case, no autoinhibition of the germination of f. oxysporum conidia occurred; moreover, cooperative effect was observed, i.e., the number of germin ... | 2004 | 15521169 |
| production of extracellular proteases by human pathogenic trichoderma longibrachiatum strains. | species belonging to the filamentous fungal genus trichoderma are well known as potential candidates for the biological control of plant pathogenic fungi and as cellulase producers of biotechnological importance. several data were published in the last decade also about the clinical importance of this genus, indicating that trichoderma strains may be potential opportunistic pathogens in immunocompromised patients. however, there is a lack of information about the potential virulence factors of c ... | 2004 | 15571068 |
| nonfatal pulmonary trichoderma viride infection in an adult patient with acute myeloid leukemia: report of one case and review of the literature. | trichoderma species have been recognized to be pathogenic in immunosuppressed hosts with increasing frequency. trichoderma species are responsible for continuous ambulatory peritoneal dialysis associated peritonitis and infections in immunocompromised patients with a hematologic malignancy or solid organ transplantation. trichoderma longibrachiatum is the most common species involved in these infections. we report the first case of nonfatal pulmonary infection caused by trichoderma viride in leu ... | 2005 | 15994049 |
| use of xylanase and arabinofuranosidase for arabinose removal from unbleached kraft pulp. | preparations of arabinofuranosidase and xylanase, respectively from aureobasidium pullulans and trichoderma longibrachiatum, were used to remove selectively xylose and arabinose from kraft pulp. the equilibrium moisture content of pulps treated with both enzymes, at varying relative humidities, revealed a consistently lower percent moisture content at all humidity set points. shorter fiber lengths indicated some deterioration when pulp was exposed to high concentrations of both enzymes. | 2005 | 16247673 |
| comparative study of potential virulence factors in human pathogenic and saprophytic trichoderma longibrachiatum strains. | potential virulence factors of 9 saprophytic and 12 clinical trichoderma longibrachiatum strains were examined in the present study, in order to compare their capacity to cause infection in humans. all of the strains were able to grow at temperatures up to 40 degrees c and at ph values ranging from 2.0 to 9.0. carbon and nitrogen source utilization experiments revealed that all of the strains were able to utilize a series of basic amino acids both as sole carbon and nitrogen sources. the mic val ... | 2005 | 16400874 |
| thptr2, a di/tri-peptide transporter gene from trichoderma harzianum. | the generation of a wide ests library and database from trichoderma harzianum cect 2413 was the base for identifying the gene thptr2, coding for a ptr family di/tri-peptide transporter. the deduced protein sequence of the thptr2 gene showed the conserved motifs and also the 12 transmembrane domains typical of the ptr transporters. the highest level of thptr2 expression was found when the fungus was grown in chitin as sole carbon source. we also found that thptr2 expression was increased when tri ... | 2006 | 16466953 |
| rapid identification of clinical trichoderma longibrachiatum isolates by cellulose-acetate electrophoresis-mediated isoenzyme analysis. | cellulose-acetate electrophoresis was used to investigate isoenzyme polymorphism among ten clinical and 11 non-clinical isolates of trichoderma. initial testing of 13 enzyme systems for activity and resolution of bands showed that seven were appropriate for identifying the different species. each of the enzyme systems investigated (glucose-6-phosphate dehydrogenase, glucose-6-phosphate isomerase, 6-phosphogluconate dehydrogenase, peptidases a, b and d, and phosphoglucomutase) was diagnostic for ... | 2006 | 16524414 |
| new approaches to the analysis of enzymatically hydrolyzed methyl cellulose. part 2. comparison of various enzyme preparations. | in this part of our studies, dealing with new approaches to the analysis of enzymatically hydrolyzed methyl cellulose, five different enzymes or enzyme preparations containing endoglucanases (from bacillus agaradhaerens cel 5a, trichoderma reesei, trichoderma viride, and two obtained from trichoderma longibrachiatum) were used to hydrolyze six different methyl celluloses (mcs). the main goal was to investigate whether enzymes could be used for determination of the heterogeneity of the substituen ... | 2006 | 16677021 |
| oxygen consumption-based evaluation of fungal activity. | a novel oxygen-based microplate assay for studying fungal activity is described. fungal activity results in a change of oxygen concentration in cvc-96 plates and thus produces a signal that enables continuous monitoring of fungal activity. in this study the oxygen consumption was different for three tested fungi, fusarium oxysporum f. sp. lycopersici, verticillium dahliae and trichoderma longibrachiatum. the assay described is a highly sensitive and reliable method for monitoring fungal activity ... | 2006 | 16876696 |
| in vitro antagonism of thielaviopsis paradoxa by trichoderma longibrachiatum. | seventy-nine trichoderma strains were isolated from soil taken from 28 commercial plantations of agave tequilana cv. 'azul' in the state of jalisco, mexico. nine of these isolates produced nonvolatile metabolites that completely inhibited the growth of thielaviopsis paradoxa on potato dextrose agar plates. these isolates were identified as trichoderma longibrachiatum on the basis of their morphology and dna sequence analysis of two genes (its rdna and translation elongation factor ef-1alpha). my ... | 2007 | 17216331 |
| generation, annotation, and analysis of ests from four different trichoderma strains grown under conditions related to biocontrol. | the functional genomics project "trichoest" was developed focused on different taxonomic groups of trichoderma with biocontrol potential. four cdna libraries were constructed, using similar growth conditions, from four different trichoderma strains: trichoderma longibrachiatum t52, trichoderma asperellum t53, trichoderma virens t59, and trichoderma sp. t78. in this study, we present the analysis of the 8,160 expressed sequence tags (ests) generated. each est library was independently assembled a ... | 2007 | 17333173 |
| engineering molecular recognition of endoxylanase enzymes and their inhibitors through phage display. | specific binding of interacting proteins generally depends on a limited set of amino acid residues located at the contact interface. we have applied a phage-display-based screening method to simultaneously evaluate the role of multiple residues of endo-beta-1,4-xylanase enzymes in conferring binding specificity towards two different endoxylanase inhibitors. seven residues of the two beta-strand 'thumb' region of trichoderma longibrachiatum endo-beta-1,4-xylanase xynii were targeted for randomiza ... | 2007 | 17393541 |
| effects of exogenous cellulase supplementation on microbial growth and ruminal fermentation of a high-forage diet in rusitec fermenters. | two incubation runs were carried out with a rusitec system to investigate the effects of 2 exogenous pure cellulases on ruminal microbial growth and fermentation of a 70:30 grass hay:concentrate (dm basis) substrate. the substrate was sprayed with buffer (control; ph = 6.5), a cellulase from trichoderma longibrachiatum (tri), a cellulase from aspergillus niger (asp), or a 1:1 mixture of both cellulases (mix) 24 h before being placed in the fermenters. enzymes were applied at a rate of 30 endoglu ... | 2007 | 17468414 |
| influence of exogenous fibrolytic enzymes and fumarate on methane production, microbial growth and fermentation in rusitec fermenters. | two incubation runs were conducted with rusitec fermenters to investigate the effects of three additive treatments (mixed fibrolytic enzymes from trichoderma longibrachiatum (fe), disodium fumarate (fum) and both additives (mix)) on rumen microbial growth and fermentation of a grass hay:concentrate (600 : 400 g/kg dm) substrate. each fermenter received daily 20 g substrate dm. application rate (per g substrate dm) was 34.3 endoglucanase, 0.57 exoglucanase, 24.7 xylanase and 5.51 amylase units fo ... | 2007 | 17475087 |
| toxicity assessment of peptaibols and contaminated sediments on crassostrea gigas embryos. | peptaibols are known membrane-modifying peptides that were recently detected in marine sediments and mussels collected from a shellfish farming area (fier d'ars, atlantic coast, france). in this investigation, embryotoxicity bioassays with oysters (crassostrea gigas) were performed to assess acute toxicity of alamethicin and different groups of peptaibols produced by a trichoderma longibrachiatum strain isolated from marine environment. c. gigas embryos appeared very sensitive to all the metabol ... | 2007 | 17582518 |
| responses of non-starch polysaccharide-degrading enzymes on digestibility and performance of growing pigs fed a diet based on corn, soya bean meal and chinese double-low rapeseed meal. | this study was conducted to investigate the effect of two distinct enzyme preparations on nutrients' digestibility and growth performance of growing pigs fed diets based on corn, soya bean meal and chinese double-low rapeseed meal (dlrm). the two enzyme preparations were enzyme r, a preparation extracted from fermentation of a non-gmo fungus penicillum funiculosum, developed for multi-grain and multi-animal species; and enzyme p, a xylanase preparation from trichoderma longibrachiatum, for pigs ... | 2007 | 17615009 |
| cloning, characterization and functional expression of an alkalitolerant type c feruloyl esterase from fusarium oxysporum. | a hypothetical protein fofaec-12213 of fusarium oxysporum was found to have high amino acid sequence identity with known type c feruloyl esterases (faes) containing a 13-amino acid conserved region flanking the characteristic g-x-s-x-g motif of a serine esterase. the putative fae from the genomic dna was successfully cloned in frame with the saccharomyces cerevisiae alpha-factor secretion signal under the transcriptional control of the alcohol oxidase (aox1) promoter and integrated in pichia pas ... | 2008 | 18414848 |
| identification of aspergillus species in oral tissue samples of patients with hematologic malignancies by in situ hybridization: a preliminary report. | a definitive diagnosis of invasive oral aspergillosis can be difficult because the culturing of tissue samples frequently fails to isolate aspergillus species. in addition, the mycelial elements of aspergillus species seen in tissue sections are histopathologically indistinguishable from those of non-aspergillus species. we analyzed the usefulness of a dna probe directed against the alkaline proteinase (alp) gene of aspergillus fumigatus for the identification of aspergillus species by the in si ... | 2008 | 18718399 |
| alternative reproductive strategies of hypocrea orientalis and genetically close but clonal trichoderma longibrachiatum, both capable of causing invasive mycoses of humans. | the common soil fungus trichoderma (teleomorph hypocrea, ascomycota) shows increasing medical importance as an opportunistic human pathogen, particularly in immunocompromised and immunosuppressed patients. regardless of the disease type and the therapy used, the prognosis for trichoderma infection is usually poor. trichoderma longibrachiatum has been identified as the causal agent in the majority of reported trichoderma mycoses. as t. longibrachiatum is very common in environmental samples from ... | 2008 | 18957598 |
| cellulase production by trichoderma longi, aspergillus niger and saccharomyces cerevisae cultured on waste materials from orange. | the wastes materials from the sweet orange (citrus sinensis) were used as substrate for the production of cellulase. the rind, the pericarp or albedo and the pulp were hydrolyzed by cellulolytic enzymes of trichoderma longibrachiatum, aspergillus niger and saccharomyces cerevisiae after they were treated with alkali and steam. the amount of glucose released from the substrates following the secretion of cellulase by the three microorganisms was measured. the orange wastes released amounts of glu ... | 2008 | 19137846 |
| identification and investigation on antagonistic effect of trichoderma spp. on tea seedlings white foot and root rot (sclerotium rolfsii sacc.) in vitro condition. | the aim of this two years research work is to recognize bio control agents and its antagonistic efficiency on sclerotium rolfsii in vitro condition. sclerotium rolfsii sacc. is a causal agent of white foot and root rot disease in many plants. this fungi causing damage in tea nurseries of tea cultivated countries especially in iran. due to importance this disease in tea nurseries of iran and impossibility using of chemical control against this damaging agent in this research, five species of tric ... | 2008 | 19137869 |
| biodiversity of trichoderma strains in tunisia. | trichoderma strains were sampled in 4 different bioclimatic zones of tunisia, a mediterranean north african country with strong climatic and edaphic variability from north to south, to assess the genetic diversity of endemic species of trichoderma and their relationship to the bioclimatic zones. in all, 53 strains were isolated and identified at the species level by analysis of their internal transcribed spacers regions 1 and 2 (its1 and its2) of the rdna cluster and (or) a fragment of the trans ... | 2009 | 19295648 |
| genetic and metabolic biodiversity of trichoderma from colombia and adjacent neotropic regions. | the genus trichoderma has been studied for production of enzymes and other metabolites, as well as for exploitation as effective biological control agents. the biodiversity of trichoderma has seen relatively limited study over much of the neotropical region. in the current study we assess the biodiversity of 183 isolates from mexico, guatemala, panama, ecuador, peru, brazil and colombia, using morphological, metabolic and genetic approaches. a comparatively high diversity of species was found, c ... | 2009 | 19439189 |
| screening pentachlorophenol degradation ability by environmental fungal strains belonging to the phyla ascomycota and zygomycota. | pentachlorophenol (pcp) bioremediation by the fungal strains amongst the cork-colonising community has not yet been analysed. in this paper, the co- and direct metabolism of pcp by each of the 17 fungal species selected from this community were studied. using hierarchical data analysis, the isolates were ranked by their pcp bioremediation potential. fifteen isolates were able to degrade pcp under co-metabolic conditions, and surprisingly chrysonilia sitophila, trichoderma longibrachiatum, mucor ... | 2009 | 19543759 |
| factors affecting ferulic acid release from brewer's spent grain by fusarium oxysporum enzymatic system. | in this study, the factors affecting ferulic acid (fa) release from brewer's spent grain (bsg), by the crude enzyme extract of fusarium oxysporum were investigated. in order to evaluate the importance of the multienzyme preparation on fa release, the synergistic action of feruloyl esterase (fae, fofaec-12213) and xylanase (trichoderma longibrachiatum m3) monoenzymes was studied. more than double amount of fa release (1 mg g(-1) dry bsg) was observed during hydrolytic reactions by the crude enzym ... | 2009 | 19592240 |
| molecular identification of trichoderma species associated with pleurotus ostreatus and natural substrates of the oyster mushroom. | green mold of pleurotus ostreatus, caused by trichoderma species, has recently resulted in crop losses worldwide. therefore, there is an emerging need for rapid means of diagnosing the causal agents. a pcr assay was developed for rapid detection of trichoderma pleurotum and trichoderma pleuroticola, the two pathogens causing green mold of p. ostreatus. three oligonucleotide primers were designed for identifying these species in a multiplex pcr assay based on dna sequences within the fourth and f ... | 2009 | 19735461 |
| enhancement of domoic acid neurotoxicity on diptera larvae bioassay by marine fungal metabolites. | peptaibols are small linear fungal peptides which are produced in the marine environment. they exhibit neurotoxicity by forming pores in neuronal membranes. this work describes their combine effect with domoic acid, a neurotoxic phycotoxin, on diptera larvae. the acute toxicity bioassay on this biological model was tested with a panel of different toxins (microbial, algal or fungal). it allowed the discrimination of neurotoxins and non-neurotoxic toxins, and an evaluation of the toxicity level ( ... | 2010 | 19941880 |
| characterization of a novel 2,4,6-trichlorophenol-inducible gene encoding chlorophenol o-methyltransferase from trichoderma longibrachiatum responsible for the formation of chloroanisoles and detoxification of chlorophenols. | de novo sequencing of eight internal peptides of purified chlorophenol o-methyltransferase, or cmt1 (before named as cpomt), from trichoderma longibrachiatum was performed by maldi-tof/tof and esi-it. a novel gene (cmt1) encoding cmt1 was cloned by using a pcr approach based on the amino acid sequence of two internal peptides. the gene (1637 bp) encoded a protein of 468 amino acids with a deduced molecular mass of 52.4 kda, and a theoretical isoelectric point of 5.93. this gene contains four int ... | 2010 | 20144725 |
| cloning, characterization, and engineering of fungal l-arabinitol dehydrogenases. | l-arabinitol 4-dehydrogenase (lad) catalyzes the conversion of l-arabinitol to l-xylulose with concomitant nad(+) reduction in fungal l-arabinose catabolism. it is an important enzyme in the development of recombinant organisms that convert l: -arabinose to fuels and chemicals. here, we report the cloning, characterization, and engineering of four fungal lads from penicillium chrysogenum, pichia guilliermondii, aspergillus niger, and trichoderma longibrachiatum, respectively. the lad from p. gui ... | 2010 | 20414651 |
| marine isolates of trichoderma spp. as potential halotolerant agents of biological control for arid-zone agriculture. | the scarcity of fresh water in the mediterranean region necessitates the search for halotolerant agents of biological control of plant diseases that can be applied in arid-zone agriculture irrigated with saline water. among 29 trichoderma strains previously isolated from mediterranean psammocinia sp. sponges, the greatest number of isolates belong to the trichoderma longibrachiatum-hypocrea orientalis species pair (9), h. atroviridis/t. atroviride (9), and t. harzianum species complex (7), all o ... | 2011 | 21666030 |
| insecticidal activity and fungitoxicity of plant extracts and components of horseradish (armoracia rusticana) and garlic (allium sativum). | to avoid environmental pollution and health problems caused by the use of traditional synthetic pesticides, there is a trend to search for naturally occurring toxicants from plants. among the compounds discussed for anti-fungal and insecticidal activity, the natural extracts from garlic and horseradish have attracted considerable attention. the objective of this study is to determine the insecticidal and anti-fungal activity of armoracia rusticana and allium sativum l. extracts against larvae of ... | 2011 | 21726146 |
| expression, characterization and structural modelling of a feruloyl esterase from the thermophilic fungus myceliophthora thermophila. | a ferulic acid esterase (fae) from the thermophilic fungus myceliophthora thermophila (synonym sporotrichum thermophile), belonging to the carbohydrate esterase family 1 (ce-1), was functionally expressed in methylotrophic yeast pichia pastoris. the putative fae from the genomic dna was successfully cloned in p. pastoris x-33 to confirm that the enzyme exhibits fae activity. the recombinant fae was purified to its homogeneity (39 kda) and subsequently characterized using a series of model substr ... | 2011 | 22012339 |
| bioremediation of heavy metals in liquid media through fungi isolated from contaminated sources. | wastewater particularly from electroplating, paint, leather, metal and tanning industries contain enormous amount of heavy metals. microorganisms including fungi have been reported to exclude heavy metals from wastewater through bioaccumulation and biosorption at low cost and in eco-friendly way. an attempt was, therefore, made to isolate fungi from sites contaminated with heavy metals for higher tolerance and removal of heavy metals from wastewater. seventy-six fungal isolates tolerant to heavy ... | 2011 | 23024411 |
| novel sources of β-glucanase for the enzymatic degradation of schizophyllan. | schizophyllan is a homoglucan produced by the fungus schizophyllum commune, with a β-1,3-linked backbone and β-1,6-linked side chains of single glucose units at every other residue. schizophyllan is commercially produced for pharmaceutical and cosmetics uses. however, surprisingly little information is available on the biodegradation of schizophyllan. enzymes that attack schizophyllan could be useful for controlled modifications of the polymer for novel applications. enrichment cultures were use ... | 2012 | 23410934 |
| modeling and structural analysis of cellulases using clostridium thermocellum as template. | cellulase is one of the most widely distributed enzymes with wide application. they are involved in conversion of biomass into simpler sugars. cellulase of trichoderma longibrachiatum, a known cellulolytic fungus was compared with clostridium thermocellum [aaa23226.1] cellulase. blastp was performed with aaa23226.1 as query sequence to obtain nine similar sequences from ncbi protein data bank. the physicochemical properties of cellulase were analyzed using expasy's protparam tool namely protpara ... | 2012 | 23251045 |
| evaluation of a parchment document, the 13th century incorporation charter for the city of krakow, poland, for microbial hazards. | the literature of environmental microbiology broadly discusses issues associated with microbial hazards in archives, but these publications are mainly devoted to paper documents. there are few articles on historical parchment documents, which used to be very important for the development of literature and the art of writing. these studies present a broad spectrum of methods for the assessment of biodeterioration hazards of the parchment document in question. they are based on both conventional m ... | 2016 | 26896133 |
| fermented whey as poultry feed additive to prevent fungal contamination. | fungal contamination of poultry feed causes economic losses to industry and represents a potential risk to animal health. the aim of the present study was to analyze the effectiveness of whey fermented with kefir grains as additive to reduce fungal incidence, thus improving feed safety. | 2014 | 24652751 |
| volatile organic compounds emitted by filamentous fungi isolated from flooded homes after hurricane sandy show toxicity in a drosophila bioassay. | superstorm sandy provided an opportunity to study filamentous fungi (molds) associated with winter storm damage. we collected 36 morphologically distinct fungal isolates from flooded buildings. by combining traditional morphological and cultural characters with an analysis of its sequences (the fungal dna barcode), we identified 24 fungal species that belong to eight genera: penicillium (11 species), fusarium (four species), aspergillus (three species), trichoderma (two species), and one species ... | 2016 | 27748984 |
| trichoderma longibrachiatum acetyl xylan esterase 1 enhances hemicellulolytic preparations to degrade corn silage polysaccharides. | supplementation of a trichoderma longibrachiatum preparation to an industrial aspergillus niger/talaromyces emersonii enzyme mixture demonstrated synergy for the saccharification of corn silage water-unextractable solids (wus). sub-fractions of the crude t. longibrachiatum preparation obtained after chromatography were analyzed regarding their hydrolytic activity. an acetyl xylan esterase 1 [axe1, carbohydrate esterase (ce) family 5]-enriched sub-fraction closely mimicked the hydrolytic gain as ... | 2014 | 24787318 |
| the hydrolysis of agro-industrial residues by holocellulose-degrading enzymes. | holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. combinations of crude enzymatic extracts from different fungal species, including aspergillus terreus, aspergillus oryzae, aspergillus niger and trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton residue to investigate the hydrolysis of holocellulose structures. a. terreus and a. oryzae were the best ... | 2012 | 24031857 |
| deep rna sequencing reveals a high frequency of alternative splicing events in the fungus trichoderma longibrachiatum. | alternative splicing is crucial for proteome diversity and functional complexity in higher organisms. however, the alternative splicing landscape in fungi is still elusive. | 2015 | 25652134 |
| efficient hydrolysis of corncob residue through cellulolytic enzymes from trichoderma strain g26 and l-lactic acid preparation with the hydrolysate. | to prepare fermentable hydrolysate from corncob residue (ccr), trichoderma strain g26 was cultured on medium containing ccr for production of cellulolytic enzymes through solid-state fermentation (ssf), resulting in 71.3 iu/g (fpa), 136.2 iu/g (cmcase), 85.1 iu/g (β-glucosidase) and 11,344 iu/g (xylanase), respectively. through a three-stage saccharification strategy, ccr was hydrolyzed by the enzymatic solution (6.5 fpu/ml) into fermentable hydrolysate containing 60.1g/l glucose (81.2% cellulos ... | 2015 | 26143000 |
| antifungal and antiproliferative activities of endophytic fungi isolated from the leaves of markhamia tomentosa. | plants harbor endophytes with potential bioactivity. markhamia tomentosa (benth) k. schum ex. engl. (bignoniaceae) is reported to possess antioxidant, anti-inflammatory and anticancer activities. | 2017 | 27937112 |
| suppression subtractive hybridization analysis provides new insights into the tomato (solanum lycopersicum l.) response to the plant probiotic microorganism trichoderma longibrachiatum mk1. | trichoderma species include widespread rhizosphere-colonising fungi that may establish an opportunistic interaction with the plant, resulting in growth promotion and/or increased tolerance to biotic and abiotic stresses. for this reason, trichoderma-based formulations are largely used in agriculture to improve yield while reducing the application of agro-chemicals. by using the suppression subtractive hybridization method, we identified molecular mechanisms activated during the in vitro interact ... | 2016 | 26705844 |
| [identification and secondary metabolites of endophytic fungal strain pr35 from paeonia delavayi]. | to identify the endophytic fungal strain pr35 separated from paeonia delavayi and study chemical constituents of its secondary metabolites. | 2012 | 22993990 |
| cyclonerol derivatives from trichoderma longibrachiatum ym311505. | a new sesquiterpene, 10,11-dihydrocyclonerotriol (1), together with two known compounds, catenioblin c (2) and sohirnone a (3), were isolated from trichoderma longibrachiatum ym311505, an endophytic fungus of azadirachta indica. their structures were characterized and identified by spectral analysis. compounds 1-3 exhibited antifungal activities against pyricularia oryzae and candida albicans. | 2014 | 24689203 |
| dissection of trichoderma longibrachiatum-induced defense in onion (allium cepa l.) against fusarium oxysporum f. sp. cepa by target metabolite profiling. | trichoderma spp. are versatile opportunistic plant symbionts that can cause substantial changes in the metabolism of host plants, thereby increasing plant growth and activating plant defense to various diseases. target metabolite profiling approach was selected to demonstrate that trichoderma longibrachiatum isolated from desert soil can confer beneficial agronomic traits to onion and induce defense mechanism against fusarium oxysporum f. sp. cepa (foc), through triggering a number of primary an ... | 2016 | 26993243 |
| [lethal effects of trichoderma longibrachiatum on heterodera avenae]. | the lethal effect of the conidia suspension of trichoderma longibrachiatum against second stage juveniles of heterodera avenae was observed under microscopic conditions and studied in vitro to preliminarily determine the potential and mechanism of the conidia suspension of t. longibrachiatum against h. avenae. microscopic observation results showed that the conidia suspension of t. longibrachiatum adhered to or parasitized on the surface of second stage juveniles, even some parasitized nematodes ... | 2014 | 25345063 |
| [parasitic and lethal action of trichoderma longibrachiatum against heterodera avenae]. | to evaluate the potential of trichoderma longibrachiatum spore suspension against heterodera avenae. | 2014 | 25252461 |
| [parasitic and lethal effects of trichoderma longibrachiatum on heterodera avenae: microscopic observation and bioassay]. | a laboratory experiment was conducted to study the parasitic and lethal effects of trichoderma longibrachiatum conidia suspension on heterodera avenae cysts. different concentrations (1.5 x 10(5)-1.5 x 10(7) cfu x ml(-1)) of t. longibrachiatum conidia suspension had strong parasitic and lethal effects on h. avenae cysts, and the effects differed significantly among the different concentrations. when treated with the t. longibrachiatum conidia suspension at a concentration of 1.5 x 10(7) cfu x ml ... | 2013 | 24483093 |
| [efficiency of trichoderma longibrachiatum t6 in the control of meloidogyne incognita and its rhizosphere colonization in cucumber]. | efficiency of different concentrations of trichoderma longibrachiatum t6 against meloidogyne incognita and its rhizosphere colonization in cucumber were determined in greenhouse experiments. the results of rhizosphere colonization experiments showed that the number of colonies in cucumber soil and root increased significantly ten weeks after inoculation with the second stage juveniles of m. incognita and different concentrations of t. longibrachiatum t6, and there was significant difference in d ... | 2016 | 27228616 |
| optimum concentrations of trichoderma longibrachiatum and cadusafos for controlling meloidogyne javanica on zucchini plants. | a factorial experiment was established in a completely randomized design to verify the effect of different inoculum levels of an iranian isolate of trichoderma longibrachiatum separately and in combination with various concentrations of cadusafos against meloidogyne javanica in the greenhouse. zucchini seeds were soaked for 12 hr in five densities (0, 10(5), 10(6), 10(7), and 10(8) spores/ml suspension) of the fungus prior to planting in pots containing four concentrations of cadusafos (0, 0.5, ... | 2016 | 27168653 |
| [endocarditis caused by trichoderma longibrachiatumin a patient receiving home parenteral nutrition]. | home parenteral nutrition (hpn) improves the quality of life of the patients although it has complications. catheter-related infections and mechanical complications are the most frequent ones. we report the case of endocarditis over catheter in a man suffering from short bowel and receiving hpn. the special features of the case are firstly the catheter was a remaining fragment on the right atrial and secondly the infection was caused by trichoderma longibrachiatum, an isolated fact regarding thi ... | 2013 | 23848126 |
| comparative analysis of the secretomes of schizophyllum commune and other wood-decay basidiomycetes during solid-state fermentation reveals its unique lignocellulose-degrading enzyme system. | the genome of schizophyllum commune encodes a diverse repertoire of degradative enzymes for plant cell wall breakdown. recent comparative genomics study suggests that this wood decayer likely has a mode of biodegradation distinct from the well-established white-rot/brown-rot models. however, much about the extracellular enzyme system secreted by s. commune during lignocellulose deconstruction remains unknown and the underlying mechanism is poorly understood. in this study, extracellular proteins ... | 2016 | 26900401 |
| trichoderma species occurring on wood with decay symptoms in mountain forests in central europe: genetic and enzymatic characterization. | the aim of this study was to explore the species diversity of trichoderma obtained from samples of wood collected in the forests of the gorce mountains (location a), karkonosze mountains (location b) and tatra mountains (location c) in central europe and to examine the cellulolytic and xylanolytic activity of these species as an expression of their probable role in wood decay processes. the present study has led to the identification of the following species and species complex: trichoderma atro ... | 2016 | 26586561 |
| phylogeny of the clinically relevant species of the emerging fungus trichoderma and their antifungal susceptibilities. | a set of 73 isolates of the emerging fungus trichoderma isolated from human and animal clinical specimens were characterized morphologically and molecularly using a multilocus sequence analysis that included the internal transcribed spacer (its) regions of the nuclear ribosomal dna and fragments of the translation elongation factor 1 alpha (tef1), endochitinase chi18-5 (chi18-5), and actin 1 (act1) genes. the most frequent species was trichoderma longibrachiatum (26%), followed by trichoderma ci ... | 2014 | 24719448 |
| 20-residue and 11-residue peptaibols from the fungus trichoderma longibrachiatum are synergistic in forming na+/k+ -permeable channels and adverse action towards mammalian cells. | certain species of the filamentous fungal genus trichoderma (e.g. trichoderma longibrachiatum and trichoderma citrinoviride) are among the emerging clinical pathogens and also the most common species in the indoor space of mould-damaged buildings. the molecules involved in its pathology are not known. in the present study, we report that 0.5-2.6 wt% of the t. longibrachiatum mycelial biomass consisted of thermostable secondary metabolites mitochondriotoxic to mammalian cells. these were identifi ... | 2012 | 22994321 |
| in vitro activity and synergism of amphotericin b, azoles and cationic antimicrobials against the emerging pathogen trichoderma spp. | the uncommon fungal pathogen trichoderma shows increasing medical importance particularly in immunocompromised patients. despite systemic antifungal therapy, prognosis of trichoderma infection is poor regardless of the type of infection and the therapy used. the aim of the present study was to evaluate the in vitro activity and synergism of double antifungal combinations including amphotericin b, voriconazole, fluconazole, chlorhexidine digluconate and akacid plus against 15 isolates of trichode ... | 2006 | 16987867 |
| suspected pulmonary infection with trichoderma longibrachiatum after allogeneic stem cell transplantation. | aspergillus and candida species are the main causative agents of invasive fungal infections in immunocompromised human hosts. however, saprophytic fungi are now increasingly being recognized as serious pathogens. trichoderma longibrachiatum has recently been described as an emerging pathogen in immunocompromised patients. we herein report a case of isolated suspected invasive pulmonary infection with t. longibrachiatum in a 29-year-old man with severe aplastic anemia who underwent allogeneic ste ... | 2017 | 28090056 |
| production of a generic microbial feedstock for lignocellulose biorefineries through sequential bioprocessing. | lignocellulosic materials, mostly from agricultural and forestry residues, provide a potential renewable resource for sustainable biorefineries. reducing sugars can be produced only after a pre-treatment stage, which normally involves chemicals but can be biological. in this case, two steps are usually necessary: solid-state cultivation of fungi for deconstruction, followed by enzymatic hydrolysis using cellulolytic enzymes. in this research, the utilisation of solid-state bioprocessing using th ... | 2017 | 28013134 |
| application of plant-growth-promoting fungi trichoderma longibrachiatum t6 enhances tolerance of wheat to salt stress through improvement of antioxidative defense system and gene expression. | soil salinity is a serious problem worldwide that reduces agricultural productivity. trichoderma longibrachiatum t6 (t6) has been shown to promote wheat growth and induce plant resistance to parasitic nematodes, but whether the plant-growth-promoting fungi t6 can enhance plant tolerance to salt stress is unknown. here, we determined the effect of plant-growth-promoting fungi t6 on wheat seedlings' growth and development under salt stress, and investigated the role of t6 in inducing the resistanc ... | 2016 | 27695475 |
| virulence and experimental treatment of trichoderma longibrachiatum, a fungus refractory to treatment. | different inocula of trichoderma longibrachiatum were tested in a murine model, and only the highest one (1 × 10(7) cfu/animal) killed all of the mice at day 15 postinfection, with spleen and liver the most affected organs. the efficacies of amphotericin b deoxycholate, liposomal amphotericin b, voriconazole, and micafungin were evaluated in the same model, with very poor results. our study demonstrated the low virulence but high resistance to antifungal compounds of this fungus. | 2016 | 27216056 |
| identification and biological activities of long-chain peptaibols produced by a marine-derived strain of trichoderma longibrachiatum. | six long-chain peptaibols, 1 - 6, were identified from agar cultures of a marine-derived trichoderma longibrachiatum rifai strain (mms151) isolated from blue mussels. the structure elucidation was carried out using electrospray ionization ion trap mass spectrometry (esi-it-ms) and gc/ei-ms. the long-chain peptaibols exhibited the general building scheme ac-aib-ala-aib-ala-aib-xxx-gln-aib-vxx-aib-gly-xxx-aib-pro-vxx-aib-xxx-gln-gln-pheol and were similar or identical to recurrent 20-residue pepta ... | 2016 | 27009018 |
| concomitant production of xylanases and cellulases from trichoderma longibrachiatum mdu-6 selected for the deinking of paper waste. | sixty fungal cultures were isolated from agricultural soil, industrial soil, forest canopy soil having decomposed leaf litter and compost samples collected from different regions of india. fifteen fungal cultures were selected qualitatively for the production of xylanase and cellulases and were identified employing its, ns and mns primers. the enzyme cocktail consisting of 3811 iu g(-1) of xylanase and 9.9 iu g(-1) of cellulase from trichoderma longibrachiatum mdu-6 was selected quantitatively f ... | 2016 | 26857368 |
| cellular and molecular insight into the inhibition of primary root growth of arabidopsis induced by peptaibols, a class of linear peptide antibiotics mainly produced by trichoderma spp. | trichoderma spp. are well known biocontrol agents that produce a variety of antibiotics. peptaibols are a class of linear peptide antibiotics mainly produced by trichoderma alamethicin, the most studied peptaibol, is reported as toxic to plants at certain concentrations, while the mechanisms involved are unclear. we illustrated the toxic mechanisms of peptaibols by studying the growth-inhibitory effect of trichokonin vi (tk vi), a peptaibol from trichoderma longibrachiatum smf2, on arabidopsis p ... | 2016 | 26850879 |