| pepstatin-insenstive acid proteases from scytalidium lignicolum. kinetic study with synthetic peptides. | a kinetic study was conducted on the acid proteases a-1 and a-2 from scytalidium lignicolum using synthetic peptides as substrates. almost maximum activity was attained with n-acylated tetrapeptides as the molecular size of substrates was increased. suitable amino acid residues were required at the p1-p2 and p1'-p2' positions [notation of schechter and berger (14)]. hydrophobic or bulky residues such as leucine were specifically required at the p1 and p1' positions, with the specificity at the l ... | 1979 | 34596 |
| first case of subcutaneous phaeohyphomycosis caused by scytalidium lignicola in a human. | a case of subcutaneous phaeohyphomycosis in a human, involving the ankle and caused by scytalidium lignicola, is described. the isolate was found to be sensitive to amphotericin b, 5-fluorocytosine, miconazole, and ketoconazole in vitro. | 1983 | 6826699 |
| nucleotide sequence of the gene encoding pepstatin-insensitive acid protease b, scytalidopepsin b, of scytalidium lignicolum. | a chromosomal dna fragment of scytalidium lignicolum that encodes the mature enzyme region of acid protease b (scytalidopepsin b), was cloned and its nucleotides sequenced. the fragment contained a 76-bp intron at the middle of the mature enzyme-coding region. the mature enzyme was composed of 206 amino acid residues with a molecular weight of 21,550. there were some discrepancies between the amino acid sequence deduced from these results and that previously established by protein sequencing. | 1996 | 8782420 |
| nucleotide sequence of the gene encoding the precursor protein of pepstatin insensitive acid protease b, scytalidopepsin b, from scytalidium lignicolum. | a chromosomal dna of scytalidium lignicolum was digested with sau3ai. the digest was self-ligated and amplified by inverse pcr procedure using primers designed based on the nucleotide sequences of up- and down-stream regions of an intron present in the scytalidopepsin b gene. analysis of the nucleotide sequence of pcr product (700 bp) showed that the enzyme is synthesized as a precursor protein consisting of the prepro- and mature enzyme regions. the deduced amino acid sequence was highly simila ... | 1998 | 9757573 |
| expression and secretion of scytalidopepsin b, an acid protease from scytalidium lignicolum, in yeast. | an expression and secretion system for scytalidopepsin b, an acid protease from scytalidium lignicolum, was constructed in yeast. saccharomyces cerevisiae ah22 was transformed with an yeast-e. coli shuttle vector, pam82, in which an yeast invertase signal segment and the cdna encoding the pro- and mature enzyme regions were inserted. the transformant was found to secret a pepstatin-insensitive acid protease, when cultured aerobically in a low phosphate (pi) medium. amino terminal amino acid sequ ... | 2000 | 10945280 |
| exocellular enzyme activity of dermatophytes and other fungi isolated from ruminants in southern iraq. | sixteen fungal species were isolated from 182 specimens collected from four ruminants (buffalo, camel, cattle and sheep) in southern iraq. fungi represented by five species of dermatophytes and eleven species of other fungi were screened for the activity of four enzymes: keratinase. proteinase, lipase and amylase. keratinase was found to be produced by all of the dermatophytes and non-dermatophytes, except for paecillomyces variottii and scytalidium lignicola. however, high keratinase activity w ... | 2001 | 11407490 |
| identification of a glutamine residue essential for catalytic activity of aspergilloglutamic peptidase by site-directed mutagenesis. | aspergilloglutamic peptidase (agp, formerly called aspergillopepsin ii) from aspergillus niger var. macrosporus is a unique acid protease recently classified to the peptidase family g1. our previous study using site-directed mutagenesis on the glutamic and aspartic acid residues of agp conserved among the g1 family suggested that glu219 and asp123 (numbering in the preproform) are important for catalytic activity. however, the asn mutant of asp123 retained weak but significant activity and there ... | 2004 | 15225626 |
| structural evidence that scytalidolisin (formerly scytalidopepsin a) is a serine-carboxyl peptidase of the sedolisin family. | scytalidopepsin a, a pepstatin-insensitive acid endopeptidase from the fungus scytalidium lignicolum was found to be a member of the sedolisin family of serine-carboxyl peptidases through analyses of the amino acid sequences of peptides derived from the reduced, pyridylethylated enzyme by enzymatic digestion. hence it should be renamed scytalidolisin (or scytalidium sedolisin). | 2008 | 18685197 |
| wood decomposing abilities of diverse lignicolous fungi on nondecayed and decayed beech wood. | we tested the decay abilities of 28 isolates from 28 lignicolous fungal species (basidiomycota, ascomycota and zygomycota) with the pure culture test. we used beech wood powder in varying moisture conditions and decay stages (nondecayed, intermediately decayed and well decayed) as substrates. the weight loss in wood powder was -0.2-17.8%. five isolates of basidiomycota (bjerkandera adusta, mycena haematopus, omphalotus guepiniformis, trametes hirsuta, trametes versicolor) caused high weight loss ... | 2011 | 21262989 |
| xylogone ganodermophthora sp. nov., an ascomycetous pathogen causing yellow rot on cultivated mushroom ganoderma lucidum in korea. | yellow rot, caused by an ascomycetous fungus having a distinctive arthroconidial anamorph, is the most destructive disease of cultivated ganoderma lucidum in korea, but the identity of the yellow rot pathogen (yrp) remains uncertain. isolates have been identified as xylogone sphaerospora (with putative anamorph sporendonema purpurascens) or as arthrographis cuboidea. therefore we used morphological features, pathogenicity tests and phylogenetic analyses of dna sequences from the nuclear ribosoma ... | 2010 | 20943517 |
| purification and properties of extracellular beta-glucosidase from scytalidium lignicola. | | 1983 | 18548555 |
| crystal structure of scytalidoglutamic peptidase with its first potent inhibitor provides insights into substrate specificity and catalysis. | scytalidoglutamic peptidase (sgp) from scytalidium lignicolum is the founding member of the newly discovered\ family of peptidases, g1, so far found exclusively in fungi. the crystal structure of sgp revealed a previously undescribed fold for peptidases and a unique catalytic dyad of residues gln53 and glu136. surprisingly, the beta-sandwich structure of sgp is strikingly similar to members of the carbohydrate-binding concanavalin a-like lectins/glucanases superfamily. by analogy with the active ... | 2007 | 17069854 |
| the peptidases from fungi and viruses. | fungi and viruses encode a variety of peptidases having a plethora of functions. many fungal peptidases are extracellular and are likely used to degrade proteins in their environment. viral peptidases are processing enzymes, intimately involved in the virus infectious cycle. the viral rna genome is translated by the host-cell machinery into a large polyprotein that is cleaved by the viral peptidases into mature capsid proteins, non-structural proteins and enzymes. i review the structure and cata ... | 2006 | 16895471 |
| the molecular structure and catalytic mechanism of a novel carboxyl peptidase from scytalidium lignicolum. | the molecular structure of the pepstatin-insensitive carboxyl peptidase from scytalidium lignicolum, formerly known as scytalidopepsin b, was solved by multiple isomorphous replacement phasing methods and refined to an r factor of 0.230 (r(free) = 0.246) at 2.1-a resolution. in addition to the structure of the unbound peptidase, the structure of a product complex of cleaved angiotensin ii bound in the active site of the enzyme was also determined. we propose the name scytalidocarboxyl peptidase ... | 2004 | 14993599 |
| onychomycosis caused by scytalidium dimidiatum. report of two cases. review of the taxonomy of the synanamorph and anamorph forms of this coelomycete. | the authors report two cases of onychomycosis in the dystrophic form, one of them involving an hiv-positive patient, provoked by scytalidium dimidiatum, previously called scytalidium lignicola. the subject is reviewed from the taxonomic viewpoint, considering the anamorph hendersonula toruloidea as a synonym of nattrassia mangiferae, and having scytalidium dimidiatum as the major synanamorph. according to many mycologists, scytalidium hyalinum may be a separate species or a hyaline mutant of scy ... | 1999 | 10602547 |
| the primary structure of pepstatin-insensitive carboxyl proteinase produced by pseudomonas sp. no. 101. | a unique carboxyl proteinase [ec 3.4.23.33] from pseudomonas sp. no. 101 is the first example of a prokaryotic enzyme which is insensitive to the classical inhibitor, pepstatin. the primary structure of the proteinase was determined by conventional methods. pseudomonas carboxyl proteinase consists of 370 amino acid residues with one disulfide bond. this enzyme has no homologous sequence with any other known carboxyl proteinase, including carboxyl proteinase b from scytalidium lignicolum, which i ... | 1995 | 8576087 |
| heat resistance of fungi from soil. | the survival of fungi from soil samples has been investigated after exposure to temperatures of 60, 70, 80 and 90 degrees c in sabouraud agar. the least resistant fungi surviving 60 degrees c for 60 min were the aspergillus (a.) niger group, chaetomium spp, penicillium spp, and scytalidium lignicola. none of these survived 70 degrees c for 10 min. the next group surviving 70 degrees c for 60 min included the a. glaucus group, byssochlamys nivea, dichotomomyces cejpii, gelasinospora spp, rhizocto ... | 1993 | 8217516 |
| dematiaceous fungal pathogens isolated from nature. | this study was conducted to demonstrate the presence of pathogenic dematiaceous fungi in nature. using hamster and mouse inoculation techniques, 43 isolates of dematiaceous fungi were recovered from 39 samples of woody plant material and soil from the virginia environment. thirteen species were identified and included 4 phialophora spp., 3 cladosporium spp., 2 exophiala spp., sporothrix sp., wangiella dermatitidis, bispora betulina, and scytalidium lignicola. evidence is presented for the first ... | 1980 | 7189579 |
| complete amino acid sequence of scytalidium lignicolum acid protease b. | the acid protease b (slb) of scytalidium lignicolum was reduced and carboxymethylated and then subjected to tryptic digestion. five fragments were isolated and some of them were further digested with alpha-chymotrypsin, thermolysin, and dilute acetic acid. the sequence analysis of these fragments and the peptides by conventional methods established the complete amino acid sequence of slb. the enzyme was composed of 204 amino acid residues with threonine and valine as its amino- and carboxyl-term ... | 1984 | 6370989 |
| isolation and amino acid sequence of a peptide containing an epoxide-reactive residue from the thermolysin-digest of scytalidium lignicolum acid protease b. | scytalidium lignicolum acid protease b, a pepstatin-insensitive acid protease, was modified by 1,2-epoxy-3-(p-nitrophenoxy)propane (epnp) with the concomitant loss of its enzyme activity, and an epnp-labeled peptide was isolated from the thermolysin-digest of the modified enzyme by hplc. the amino acid sequence of the peptide was determined to be ile-leu-glu-thr-gly, which corresponds to the sequence of residue nos. 51-55 of the enzyme. the results of treatment of the labeled peptide with hydrox ... | 1986 | 3519605 |
| interdigital, cutaneous phaeohyphomycosis due to scytalidium lignicola pesante 1957. a case report. | | 1988 | 3231233 |
| [phaeohyphomycosis of the skin caused by scytalidium lignicola (the first case of a clinical observation in the ussr)]. | the authors made a short review of literature on phaeohyphomycosis and described the first case of this disease in the ussr. it was diagnosed on the basis of mycological investigation. this type of investigation was recommended for patients at risk of developing opportunistic infections. | 1988 | 3065961 |
| cutaneous phaeohyphomycosis caused by scytalidium lignicola - report of the first three cases in brazil. | | 1989 | 2319947 |
| toenail infection caused by onychocola canadensis gen. et sp. nov. | three cases of great toenail infection are described in which a slow-growing arthroconidial hyphomycete was isolated repeatedly and in pure culture. direct microscopy revealed hyaline, round to barrel-shaped arthroconidia, hyaline hyphae of varying width, and broad thick-walled brownish hyphae. three additional isolates were obtained from clinical specimens, for which the results of direct microscopy were unknown or negative. the fungus was resistant to cycloheximide, sensitive to common antifun ... | 1990 | 2149386 |
| the primary structure of aspergillus niger acid proteinase a. | the complete amino acid sequence of the acid proteinase a, a non-pepsin type acid proteinase from the fungus aspergillus niger var. macrosporus, was determined by protein sequencing. the enzyme was first dissociated at ph 8.5 into a light (l) chain and a heavy (h) chain, and the l chain was sequenced completely. further sequencing was performed with the reduced and pyridylethylated or aminoethylated derivative of the whole protein, using peptides obtained by digestions with staphylococcus aureus ... | 1991 | 1918059 |