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the development of new iron-chelating drugs.2,3-dihydroxybenzoic acid has been identified as a potentially useful iron-chelating drug. accordingly, we have evaluated a series of derivatives of hydroxylated benzoic acids for their ability to induce iron excretion in the iron-overloaded rat. in addition, we have examined a number of hydroxamic acids and some other naturally occurring iron-chelating agents. of the 26 benzoic acid derivatives studied, none appeared to be more effective than 2,3-dihydroxybenzoic acid, for reasons which are dis ...19761255491
[effect of yeast mannan on the activity and kinetic patterns of alpha amylase from rat blood serum].the mannan from rhodotorula rubra activated alpha-amylase of blood serum in vivo. after intravenous administration of the polysaccharide into rats kinetic patterns of the enzyme were altered. the effect of mannan on various molecular forms of alpha-amylase was also studied.19751216753
[effect of yeast mannans on aldolase of rat blood serum].the activity of aldolase in rat blood serum was studied in experiments in vitro and in vivo as affected by extracellular mannan of rhodotorula rubra. it was established that in vitro the polysaccharide has an inhibitory effect in the enzyme and in vivo, vice versa, activates it. the maximal activation is observed against a background of the v increase and km decrease. by the preparative electrophoresis in 7.5% polyakrylamide gel seven isoenzymes of aldolase are obtained. after introduction of th ...19751209756
the atmospheric fungal flora of the athens metropolitan area.in the research programme of the department of microbiology of the athens university the nature of the mycological flora of the athenian air was studied. the research took place during the calendar year 1971. the open air was sampled twice weekly from two observation stations. the open plate technique was used, petri dishes containing sabourand's agar being exposed for 15 minutes. a total of 180 plates were exposed, and 1714 fungal colonies were isolated; these were subcultured and identified as ...19751207717
rhodotorula rubra--cause of eye infection. 19751169685
endocarditis caused by rhodotorula successfully treated with 5-fluorocytosine.a 7-year-old boy suffering from aortic regurgitation and mitral stenosis and regurgitation was admitted with endocarditis caused by rhodotorula pilimanae and was treated successfully with orally administered antifungal agent, namely 5-fluorocytosine (5-fc). a dose of 100 mg per kg body weight, divided into four equal parts, was prescribed. after a prolonged febrile period his temperature dropped to normal on the fourth day of 5-fc therapy. review of the published reports disclosed few cases of e ...19751167783
mycotic flora of the interdigital spaces of the human foot: a preliminary investigation.a qualitative and quantitative study of the mycotic flora of the interdigital spaces of 27 male volunteers yielded 1,291 moulds and 598 yeasts. concurrently, a study of garden soil was conducted in order to obtain data concerning the transient-resident status of the fungi recovered from the feet. of the 120 genera and species of fungi isolated, 51 were recovered from the volunteers, 53 from the soil, and 16 from both categories. the most commonly recovered fungi from the toewebs, in order to occ ...19751143314
aromatic metabolism in the fungi. growth of rhodotorula mucilaginosa in p-hydroxybenzoate-limited chemostats and the effects of growth rate on the synthesis of enzymes of the 3-oxoadipate pathway.rhodotorula mucilaginosa was grown in p-hydroxybenzoate-limited chemostats over the dilution rate (d) range 0.01 to 0.17 h-1 and growth was adequately described by the monod theory when maintenance energy requirements were considered. the p-hydroxy-benzoate affinity constant, k8, had the relatively high value of 270 mg/i. the yield from p-hydroxbenzoate varied with dilution rate but was constant above d equal 0.07 h-1 at 0.56 g yeast/g substrate utilised. the maintenance coefficeint for growth o ...19751090273
[fungemia caused by rhodotorula mucilaginosa in relation to total parenteral nutrition].fungal infections are nowadays more common in clinical practice. the most frequently isolated fungi are candida and cryptococcus. infection due to rhodotorula mucilaginosa is very uncommon. we describe here our experience with r. mucilaginosa fungemia, with emphasis on total parenteral nutrition related episodes.19921489796
meningitis caused by rhodotorula.a case of rhodotorula meningitis in a 21 year old male compromised with acute lymphoblastic leukemia is reported. the yeast was identified as rhodotorula rubra (demme) lodder.19761069381
lipid composition of 30 species of yeast.the detailed composition of cellular lipid of more than 23 species of yeast has been determined quantitatively by thinchrography on quartz rods, a method previously used for estimating cellular lipids of seven species of yeast. that data was fortified by neutral and phospholipid quantitations on 30 species of yeast cells. most of the test organisms contained 7-15% total lipid and 3-6% total phospholipid per dry cell weight, except for the extremely high accumulation of triglycerides in two speci ...19761011938
[study of the rhodotorula rubra cell wall. vii influence of chloramphenicol on its morphology and phosphatase activity (author's transl)].rh. rubra grown in the presence of chloramphenicol (500 mug/ml of medium) displayed morphological and biological changes, including formation of aberrant mitochondria with altered cristae, increase of the ratio fundamental element/mucilaginous sheath and electron-transparent regions in the cell walls. after treatment of the yeast with trypsin or beta-glucanase complex, acid and alkaline phosphatases remained localised within the cell wall, but the effect of the degradative enzymes on the activit ...1976943241
[studies on the cell wall of yeast of the genus rhodotorula. x. isolation and purification of cell-wall glycoproteins from rhodotorula rubra (author's transl)].following ethylene diamine treatment of the cell wall of rhodotorula rubra, a water soluble fraction has been isolated. this fraction can be resolved into three glycoproteins and one protein. the major part is a glycoprotein, purified to homogeneity which has a molecular weight of 64 000. the glyco-part of this protein contains mannose, glucose and one residue of glucosamine. after pronase treatment, the presence of an ""asparaginyl-n-acetylglucosamine" linkage is established by the existence of ...1976943181
[infrared spectrum of yeast cell walls].infrared spectra of whole cells and cell walls were studied with schizosaccharomyces pombe, endomyces magnusii. rhodotorula rubra, and candida lipolytica. qualitative analysis of the spectra and comparison of the spectra recorded for the cells and for their walls led to a conclusion that, contrary to the common viewpoint, an ir spectrum of the whole cell of any yeast species was determined by the components contained in the cell rather wall than by all components of the cell.1977895561
taxonomic study and fermentation of producing organism and antimicrobial activity of mildiomycin.a taxonomic study of strain b-98891, which produced an antibiotic effective against powdery mildew of barley, identified it as streptoverticillium rimofaciens. on agar media the antibiotic, which was named mildiomycin, was only weakly active against most fungi and bacteria tested. however, it inhibited some mycobacterium and rhodotorula, and it showed excellent control of powdery mildew of barley plants in greenhouse tests at concentrations between 31.2 and 62.5 ppm. rhodotorula rubra ifo 0907 w ...1978681232
[lipolytic activity of osmotolerant yeasts].the ability to hydrolyse olive oil, cod-liver oil, caviar oil, and yeast oil was studied in osmotolerant yeasts candida mogii 2, debaryomyces hansenii 8, and rhodotorula rubra 9, isolated from salted caviar. the lipolytic activity was manifested at 5, 15 and 26 degrees c and ph 4.0--9.2, even in the presence of high concentrations (0.5--3 m) of such chlorides as kcl, nacl and cacl2. the lipolytic activity of s variant of d. hansenii 8 was higher than the activity of its r variant.1978661632
the microbial ecology of soybean soaking for tempe production.soybeans soaked in tap water for 24 to 36 h at 20, 30 or 37 degrees c underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/ml (depending on temperature) and a reduction of ph from 6.5 to 4.5. lactobacillus casei, streptococcus faecium, staphylococcus epidermidis and streptococcus dysgalactiae dominated the fermentation but, significant contributions were also made by klebsiella pneumoniae, klebsiella ozaenae, enterobacter cloacae, enterobact ...19892518226
the fine structure of psychrophilic and mesophilic rhodotorula rubra.transmission electron microscopy of thin sections of two mesophilic and a psychrophilic rhodotorula rubra revealed structures normally associated with yeast. the capsule of all three strains was thicker in the cells grown in glucose than those grown in glucose-free media. the cell wall of all three strains showed more lamellae in the cells grown in a medium containing glycerol. budding was of the rhodoturula type. endoplasmic reticula running parallel to the nucleus were commonly observed in the ...1979575628
reversible bioconcentration of monochlorobiphenyls by rhodotorula rubra: correlations with aqueous solubility of substrate. 1979574035
taxonomic implications of rhodotorula rubra isolates from polluted sea water in rio de janeiro, brazil.sixty two strains of rhodotorula rubra which were all isolated from marine and estuarine waters of rio de janeiro were found variable for 19 to 32 carbon assimilation tests used in yeast taxonomy. two r. rubra strains which had latent assimilation of maltose and melizitose appeared to be intermediate with r. pilimanae. strains of r. glutinis var dairenensis which differed from r. rubra only by weak assimilation of nitrate appeared to be intermediated between these two species. our physiological ...1979572568
the distribution of pyrophosphatidic acid in nature.the occurrence of a novel phospholipid, pyrophosphatidic acid, in the lipid extracts of yeasts (23 species), bacteria (e. coli), algae (chlorella), mammalia (human, rabbit, guinea pig, and mouse), insect (cockroach), fish (carp), mollusc (clam), and spermatophyta (spinach) was investigated. pyrophosphatidic acid was found exclusively in the lipid extracts of several kinds of yeast species, but not in other normal living species (animals, plants, and microorganisms) so far investigated. all of th ...1975175047
phosphate-limited continuous culture of rhodotorula rubra: kinetics of transport, leakage, and growth.the phosphate-limited growth kinetics of rhodotorula rubra, a small yeast of marine origin, were examined by analysis of 32p distributions in continuous cultures. isotope relaxation procedures were used to identify unidirectional flows of pi and organic phosphate among compartments modeled during growth. the concentrations of phosphates in these compartments at various growth rates were used, together with attendant flows, to produce a mathematical model of growth. both pi and phosphate-containi ...197937231
coordination chemistry of microbial iron transport compounds: rhodotorulic acid and iron uptake in rhodotorula pilimanae.the mechanism by which iron uptake is facilitated by the siderophore rhodotorulic acid (ra) in the yeast rhodotorula pilimanae was investigated with radioactively labeled fe and ra and kinetically inert, chromic-substituted ra complexes. the weight of the evidence supports a model in which ra mediates iron transport to the cell but does not actually transport iron into the cell. it is proposed that ra exchanges the ferric ion at the cell surface with a membrane-bound chelating agent that complet ...197830750
acid phosphatase of the yeast rhodotorula rubra. purification and properties of the enzyme.1. acid phosphatase from the yeast rhodotorula rubra was purified 44-fold. the purification procedure involved mechanical disruption of cells, precipitation with ethanol, chromatography on deae- and cm-cellulose. 2. the purified enzyme is homogeneous in polyacrylamide gels at ph 4.5, 9.5 and 8.4. carbohydrate content accounts for 57% of the total weight. the optimum ph is at 4.0-4.6, and the enzyme is stable over ph range from 2.6 to 6.0. full activity was retained on 60-min incubation at 50 deg ...197717985
rhodotorula fungaemia: a life-threatening complication of indwelling central venous catheters.a 30-year-old woman receiving total parenteral nutrition via an indwelling central venous catheter for an intestinal motility disorder developed fever, tachycardia, tachypnea, and hypotension. multiple blood cultures drawn through the catheter prior to these events, as well as a peripheral blood culture obtained earlier, grew the red yeast rhodotorula rubra. the patient was critically ill for over one month but eventually recovered with therapy including the systemic antifungal agents amphoteric ...19921302803
identification and characterization of mitochondrial translation products in various yeasts and in prototheca zopfii.mitochondrial genomes of different eucaryotes are not all alike. we have examined mitochondrial translation products in a number of yeasts (candida krusei, hansenula saturnus, rhodotorula glutinis, rhodotorula rubra, torulopsis glabrata and saccharomyces cerevisiae) and in prototheca zopfii, a colorless alga, in order to determine whether certain proteins are invariably synthesized within mitochondria, how different these proteins are, and what additional proteins, if any, might be synthesized b ...19852836096
[biological features and experimental pathogenicity of candida strains isolated by hemoculture at the hospital infantil de méxico "federico gómez"].in a period of 15 months (1990-1991) it was carried out 5781 blood cultures in which 180 strains of yeast-like were isolated. 116 of these strains were selected for biochemical classification, cellular and colonial morphology and experimental determination of virulence in mice. most of the strains were classified as candida albicans (60%) and the others were c. tropicalis (15.5%); c. guillermondii (10.3%); t. glabrata (6.8%); rhodotorula rubra (3.4%); cryptococcus neoformans (2.58%) y c. parapsi ...19921345115
cytochrome p-450-dependent catabolism of triethanolamine in rhodotorula mucilaginosa.the yeast rhodotorula mucilaginosa was able to grow in media containing triethanolamine or diethanolamine as the sole nitrogen source. during growth in the presence of triethanolamine, extracts of yeast cells contained increased levels of cytochrome p-450 dependent monooxygenase which catalyzed the oxidative n-dealkylation of aminoalcohols. formation of diethanolamine, ethanolamine and glyoxylate from triethanolamine was demonstrated, and the identity of the products was verified by thin layer c ...19911368151
[limulus test (factor g) and polysaccharides from fungus].picogram quantifies per ml of endotoxin showed a positive limulus test. the concentration more than ten pg/ml of curdlan ((1-3)-beta-d-glucan) was also positive to a conventional limulus test, which contains a factor g. one nanogram per ml of cm-curdlan was positive, but higher concentration of 10 pg/ml of cm-curdlan decreased its optical density of the conventional limulus test. more than one hundred ng/ml of mannan from saccharomyces cerevisiae did not react with factor g. however, a culture s ...19921402105
rhodotorula rubra contamination in fiberoptic bronchoscopy.rhodotorula rubra was recovered in 18 bronchoscopic specimens from 15 patients from may to november 1987. one hundred and twenty-one bronchoscopies were performed during that period by two bronchoscopists (w. w.; r.d.) at letterman army medical center in san francisco. isolation of r rubra occurred in 11 bronchoalveolar lavage (bal) specimens, four bronchial washes, and three transbronchial biopsies. clinical infection was not present in any of these patients, although five were immunocompromise ...19921424874
[kininases from yeasts (author's transl)].practically no studies are available on the presence of kinin-decomposing enzymes in yeasts. different preparations (intact washed cell suspensions, cell-homogenised blastospores, nutrient media, supernatant of raw fungus suspensions) of candida strains sampled from foci of disease and from the environment were studied qualitatively and quantitatively fro the presence of kinin-inactivating enzymes. the parameter measured is the time-dependent inactivation of bradykinin by the test strain prepara ...19751154909
characterization of trehalase in rhodotorula rubra.trehalase activity in rhodotorula rubra was found to be bound to the particulate fraction of a cell-free extract in contrast with the soluble trehalase activity of saccharomyces cerevisiae. the enzyme was strongly repressed by glucose and derepressed during growth on maltose, trehalose and glycerol. this increase in activity was due to a "de novo" synthesis as seen by inhibition with cycloheximide, a mechanism not described for saccharomyces cerevisiae. catabolite inactivation by addition of glu ...19921482405
causes of conductance change in yeast cultures.the conductance change due to growth of saccharomyces cerevisiae y112, zygosaccharomyces bailii m and rhodotorula rubra ncyc 63 in culture media containing glucose, tartrate ph buffer and ammonium ions as sole nitrogen source was compared with that in a medium containing l-asparagine as sole nitrogen source. decreases in conductance were observed in glucose-ammonium cultures of all three yeasts while little change occurred in cultures with l-asparagine as sole nitrogen source. this supports the ...19921541598
analysis of the gene for phenylalanine ammonia-lyase from rhodosporidium toruloides.we have cloned and sequenced the pal gene encoding phenylalanine ammonia-lyase (pal) from rhodosporidium toruloides strain cbs14. our data imply a different start codon and thus a different amino acid sequence for the n-terminus of pal as compared to the previously published sequence for pal from r. toruloides strain if00559. primer extension analysis shows three transcription initiation sites with non-translated leaders of 24-35 nucleotides. upstream of these initiation sites is a long stretch ...19911773059
rhodotorula rubra ventriculitis.we describe a case of post-operative ventriculitis in an immunocompetent patient caused by an unusual organism, namely rhodotorula rubra. the patient was treated successfully with antifungal agents.19883351318
silver tolerance and accumulation in yeasts.debaryomyces hansenii (ncyc 459 and strain 75-21), candida albicans (3153a), saccharomyces cerevisiae (x2180-1b), rhodotorula rubra (ncyc 797) and aureobasidium pullulans (imi 45533 and atcc 42371) were grown on solid medium supplemented with varying concentrations of agno3. although ag+ is highly toxic towards yeasts, growth on solid media was still possible at ag concentrations of 1-2 mm. further subculture on higher ag concentrations (up to 5 mm) resulted in elevated tolerance. the extent of ...19911878280
pseudoepidemic of rhodotorula rubra in patients undergoing fiberoptic bronchoscopy. 19902115899
[changes in the mutagenic effect of a medium containing aflatoxin b1 as a result of the yeast activity].changes in the mutagenic activity of the nutrient medium containing aflatoxin br as a result of living activity of the yeast rhodotorula mucilaginosa i b has been studied. reduction of mutagenic activity of the medium when tested on the strains salmonella typhimurium ta98 (6.3 times fold), ta100 (2.2 times fold), ag262 (2 times fold) has been revealed. the data obtained leads to the conclusion that the technology, based on the use of the indicated strain of the yeast for the treatment of food an ...19902128746
growth of opportunistic yeasts on vitamin-free solid medium.the growth of some opportunistic yeasts (candida albicans, cryptococcus neoformans, torulopsis glabrata, rhodotorula rubra, geotrichum candidum, trichosporon cutaneum, saccharomyces cerevisiae) was essayed in a solid minimal synthetic medium without vitamins. from the preliminary results some species (candida albicans, cryptococcus neoformans, rhodotorula rubra, geotrichum candidum and saccharomyces cerevisiae) seem to be autotrophic regarding the growth factor considered indispensable for their ...19902191196
oral yeasts in patients with cancer of the mouth, before and during radiotherapy.the yeasts of patients with oral cancer has been studied before and during xr-therapy. gram and pas smears revealed an increase of yeast-like structures, during treatment, from 56% to 66% of the cases. before radiotherapy oral yeasts were isolated from 56% of the patients with cancer represented by candida albicans (30%); c. tropicalis (12%); c. glabrata and c. krusei (4%), besides six other different species (2%). during radiotherapy yeasts were isolated in 72% of the cases, as follow: c. albic ...19902293033
yeasts and fungi occurring in ensiled whole-crop maize and other ensiled vegetable crops.the yeast flora of whole-crop maize ensiled for two weeks was predominated by candida holmii, c. lambica, c. milleri, hansenula anomala and saccharomyces dairensis. inoculation with other yeast species reported in the literature to prevail in maize or wheat silages did not alter the yeast flora. at 25 or 30 degrees c the ascomycetous fermentative species found at 20 degrees c were accompanied with ascomycetous non-fermentative fungi, i.c. exophiala jeanselmei and verticillium psalliotae, by the ...19902321936
pseudoepidemic of rhodotorula rubra in patients undergoing fiberoptic bronchoscopy.between march and june 1988, rhodotorula rubra was isolated from the bronchial washings of 30 of 56 (54%) patients undergoing bronchoscopy at a north carolina community hospital. pulmonary disease consistent with invasive fungal pneumonia was not apparent for any patient. repeat sputum cultures were performed on 11 patients, none of whom were positive for r rubra. investigation revealed fungal contamination of two brushes used to clean the bronchoscope channels and one positive sample of the tub ...19892584679
the role of carotenoids in preventing oxidative damage in the pigmented yeast, rhodotorula mucilaginosa.rhodotorula mucilaginosa is an obligate aerobic yeast which contains a high concentration of carotenoid pigment. to test whether carotenoids are able to protect r. mucilaginosa against oxidative injury, yeast cells in liquid culture were incubated with duroquinone (dq) (100 microm), a redox-cycling quinone known to generate intracellular o2-. or were grown in a hyperoxic atmosphere (80% o2) under conditions where carotenoid concentrations were altered either intracellularly or extracellularly. n ...19892650623
yeast-suspension as soiling matter in disinfectant testing.using the kelsey-sykes capacity-test, it was found that a sterile yeast suspension used to simulate 'dirty' conditions, gave an increased effect of chloramine t against the fungi candida albicans, aspergillus fumigatus, geotrichum candidum and penicillium sp. compared with the effect under 'clean' conditions. this effect was not found with the fungus rhodotorula rubra nor on the various bacteria tested. the enhanced effect was found with respect to both chloramine t and chloramine b, but not wit ...19892661973
biotransformation and detoxification of t-2 toxin by soil and freshwater bacteria.bacterial communities isolated from 17 of 20 samples of soils and waters with widely diverse geographical origins utilized t-2 toxin as a sole source of carbon and energy for growth. these isolates readily detoxified t-2 toxin as assessed by a rhodotorula rubra bioassay. the major degradation pathway of t-2 toxin in the majority of isolates involved side chain cleavage of acetyl moieties to produce ht-2 toxin and t-2 triol. a minor degradation pathway of t-2 toxin that involved conversion to neo ...19892705769
microbiological transformations. xi. the use of immobilized rhodotorula mucilaginosa cells to reduce some ketones.rhodotorula mucilaginosa cells were immobilized by polymerizing acrylamide with the addition of a cross-linked agent, a catalyst and an initiator. this gel was used for the stereospecific reduction of acetophenone and alpha-acetylnaphthalene resulting chromatographically pure alcohols with absolute configuration s. when karen-3-dion-2,5 was used as substrate, four products were obtained. androstenolone was reduced with 30% yield. activity of the immobilized cells could be regenerated by glucose.1979157727
evaluation of the pro-lab id ring system for the identification of medically important yeasts.we evaluated 151 coded isolates of medically important yeast species belonging to the genera candida, cryptococcus, geotrichum, rhodoturula, saccharomyces and torulopsis using the newly developed rapid pro-lab identification ring, pl 960 system (plid-ring). all isolates were concurrently identified by the api 20c and conventional procedures comprising macro- and micromorphology, assimilation and fermentation of various carbon and nitrogen compounds. the plid-ring system identified isolates of ca ...19921406902
31p-nmr evidence for cytoplasmic acidification and phosphate extrusion in syringomycin-treated cells of rhodotorula pilimanae.31p-nmr spectroscopy was used to investigate the effects of the phytotoxin, syringomycin, on phosphate metabolism and intracellular ph changes in the yeast rhodotorula pilimanae. syringomycin, at levels between 20 and 60 units per 10(8) cells, caused a cellular efflux of orthophosphate. at 40 and 60 unit per 10(8) cells, the efflux was accompanied by a decrease in polyphosphate and an acidification of the cytoplasm. at low temperatures (5 degrees c) and with 75 units per 10(8) cells, these effec ...19892920179
[experimental studies relative to the persistence of several facultative pathogenic fungi in river water].under laboratory conditions survival time of 3 fungi species (candida albicans, geotrichum candidum, rhodotorula rubra) in river water of various quality and temperature has been studied. the mean (and the maximum) survival time was as follows: 82 (240) d for c. albicans, 210 (578) d for g. candidum and 333 (606) d for r. rubra. differences concerning survival time were recorded in dependence on temperature and quality of water. all the 3 strains were multiplied periodically. variants of culture ...19883066060
aspects of mitochondriogenesis in yeast. ii. the effect of chloramphenicol on rhodotorula mucilaginosa. 19715105336
nucleotide sequence of gene for phenylalanine ammonia-lyase from rhodotorula rubra. 19883205749
formation of a biotin precursor, pimelic acid, in yeasts from c18 fatty acids.the biosynthesis of biotin-vitamers from c18 fatty acids in the resting cell reaction system of yeasts was investigated. the formation of pimelic acid (a biotin precursor) and biotin-vitamers (a mixture of 7-keto-8-aminopelargonic acid and dethiobiotin) from linolenic, linoleic, and oleic acids was observed in certain yeasts belonging to the genera cryptococcus, candida, rhodotorula, and trichosporon. metabolites from linolenic acid were analyzed by gas-liquid chromatography and mass spectrometr ...19883236079
aspects of the stereochemistry of torularhodin biosynthesis.1. the incorporation of [2-(14)c]acetate, [2-(14)c]mevalonic acid and [2-(14)c,2-(3)h(2)]-mevalonic acid into torulene and torularhodin by rhodotorula rubra and rhodotorula glutinis was studied. 2. a recovery of 14.3% of the label was obtained on decarboxylation of the torularhodin biosynthesized from [2-(14)c]mevalonic acid. 3. an analysis of the (3)h/(14)c ratio in torularhodin gave a value of 9.44:8. 4. these results, obtained by different experimental techniques, show that the reactions in t ...19705465554
[mannan sulfates--inducers of plant resistance to viral infection].mannan sulphates (ms) synthesized on the basis of extracellular linear mannan (lm) of rhodotorula rubra induce resistance of immune-580 tobacco and thornapple (datura stramonium) to tobacco mosaic virus (tmv). the resistance is manifested in a decrease in the number and/or size of viral local lesions (ll) in ms-treated (mg/ml) leaf halves. the reduction in the ll size does not seem to be due to the direct inhibition of tmv multiplication by the polysaccharide as the virus accumulation in the tis ...19883247692
an improved method for protoplast formation and its application in the fusion of rhodotorula rubra with saccharomyces cerevisiae.protoplasts from various strains of red-pigmented yeasts were generated at high frequency using improved procedures. the use of sulphur-containing amino acids and 2-deoxyglucose in growth media led to impaired cell wall synthesis and rendered cells very susceptible to treatment with mercapto-ethanol and various lytic enzymes. use of individual lytic enzymes separately resulted in relatively low frequencies of protoplasts from most of the red yeasts examined, whilst use of beta-glucuronidase, nov ...19873307677
orthogonal-field-alternation gel electrophoresis banding patterns of dna from yeasts.chromosomal dnas from various yeast species were separated by orthogonal-field-alternation gel electrophoresis (ofage). to this end we developed a spheroplasting and lysis method to obtain intact dna from both ascomycetous and basidiomycetous yeasts. the ofage banding patterns of 22 ascomycetous and four basidiomycetous yeast strains were compared. the strains represented species from the genera: brettanomyces, candida, cryptococcus, filobasidiella, geotrichum, hansenula, kluyveromyces, pachysol ...19863333308
[effect of rhodotorula rubra yeast mannan on the molecular heterogeneity of rat blood serum aldolase in the dynamics of tumor growth]. 1976946917
[study of the fractions and fibrinolytic activity of fungal mannan].characteristics of the fraction composition of extracellular mannan produced by rhodotorula rubra are presented. various lots of the polysaccharide mainly contained two fractions similar by their chemical structures and differing in the solution relative viscosity. hplc was used for determining the molecular weight of the samples. in the isolated fractions it differed 3-5 fold. relationship between the fibrinolytic activity of the polysaccharide and its molecular weight was revealed. the samples ...19883415392
incidence of yeasts in pregnant and non-pregnant women in nigeria.the yeast flora of vagina and associated sites (cervix, urethra, perineum and rectum) in 224 pregnant and 106 non-pregnant nigerian women was investigated. a variety of yeasts represented by 12 species were isolated. candida albicans was the most frequently isolated species in both groups, accounting for 63.6% of the isolates from pregnant women and 44.8% of the isolates from non-pregnant women. other species of yeasts infrequently or occasionally encountered were torulopsis glabrata, c. tropica ...19892733724
new spectrum of fungal infections in patients with cancer.we report on 44 cancer patients who had serious infections with unusual fungal pathogens and who were cared for at our cancer center between 1974 and 1986. twelve different fungal species accounted for these infections, including trichosporon beigelii, fusarium species, geotrichum candidum, curvularia species, drechslera species, penicillium species (but not penicillium marneffei), rhodotorula rubra, pseudallescheria boydii, pichia farinosa, torulopsis pintolopesii, saccharomyces cerevisiae, and ...19892749101
yeasts associated with the white shrimp penaeus schmitti, sediment, and water of sepetiba bay, rio de janeiro, brasil.samples were collected in a polluted site with mean fecal coliform counts of the water being 9.0 x 10(2)/100 ml and less polluted areas including commercial fishing areas with fecal coliforms typically below 2.0 x 10(1)/100 ml. yeast populations from the polluted site were 4.6 x 10(2) to 9.2 x 10(3)/g of shrimp intestine, 1.0 x 10(1) to 2.4 x 10(2)/g of sediment and 4.0 x 10(-1) to 4.6 x 10(1)/ml of water, and in the less polluted site 3.0 x 10(1) to 2.3 x 10(2)/g of intestines and 3.0 x 10(-1) ...19892750315
continuous culture of rhodotorula rubra: kinetics of phosphate-arsenate uptake, inhibition, and phosphate-limited growth.the pink yeast rhodotorula rubra of marine origin was found to be capable of extended growth at very low phosphate concentrations (k(0.5) = 10.8 nm). average intracellular phosphate concentrations, based on isotope exchange techniques, were 15 to 200 nm, giving concentration gradients across the cell envelope of about 10(6). sensitivity to metabolic inhibitors occurred at micromolar concentrations. inability of the phosphate transport system, k(s) = 0.5 to 2.8 mum, v(max) = 55 mumoles per g of c ...19734690960
survey for fusaria that elaborate t-2 toxin.of the 136 strains of fusarium examined, t-2 toxin was confirmed by thinlayer chromatography in 13 of the 21 extracts that inhibited either rhodotorula rubra or pencillium digitatum.19725064987
growth hormesis: a by-product of control.data from experiments, in which colonies of a hydroid, laomedea flexuosa, were exposed to a range of cu2+ concentrations and a marine yeast, rhodotorula rubra, was exposed to a range of cd2+ concentrations, not only exhibit hormesis, but also suggest how its occurrence in growth experiments might be explained. when growth data are considered as normalized specific rates against a time base, their oscillatory form indicates the output of a growth regulatory mechanism whose behaviour can be used t ...19872883157
microbial models of mammalian metabolism: microbial reduction and oxidation of pentoxifylline.fourteen microorganisms, including fungi, yeasts, and bacteria, were screened for their ability to metabolize the xanthine drug pentoxifylline. thirteen cultures either reduced the drug to the alcohol metabolite or oxidatively cleaved the ketonic side chain to homologous carboxylic acid metabolites. the alcohol metabolite was the predominant or sole metabolite in all organisms, with conversions ranging from 6 to 91%. preparative-scale production of the alcohol metabolite with rhodotorula rubra ( ...19846486781
preparation of chiral, highly functionalized cyclopentanes and its application to the synthesis of prostaglandin e1.this paper describes the preparation of the polyfunctionalized cyclopentane ((+/-)-5) by silica gel-catalyzed air-oxidation, and its kinetic resolution by means of microbial reduction with rhodotorula rubra to afford optically pure (-)-5 (greater than 99% ee). starting with (-)-5, a new route to prostaglandin e1 was established.19892598306
biological assays for two mycotoxins produced by fusarium tricinctum.a survey was made to detect microorganisms useful for assaying butenolide [4-acetamido-4-hydroxy-2-butenoic acid gamma-lactone] and t-2 toxin [4beta, 15-diacetoxy-8alpha-(3-methylbutyryloxy)-12,13-epoxytricothec -9-en-3alpha-ol]. these mycotoxins produced by strains of fusarium tricinctum have been implicated in mycotoxicosis of livestock. although butenolide proved to be a very weak antibiotic, assay discs containing 100 mug of this toxin inhibited sprillum serpens nrrl b-2052, vibrio tyrogenus ...19705485724
rhodotorula rubra peritonitis in a child undergoing intermittent cycling peritoneal dialysis. 19883335817
rhodotorula mucilaginosa dermatitis on feathered parts of chickens: an epizootic on a poultry farm. 19705464037
microbial transformation. i. microbial transformation of androstenedione and testosterone by rhodotorula mucilaginosa (jörg) harrison. 19695374566
correlation of biological to chromatographic data for two mycotoxins elaborated by fusarium.thirty-seven identified strains of fusarium, most of them isolated from fescue grass, were tested for their ability to elaborate mycotoxins in laboratory culture. the presence of the toxins was determined by infrared light, thin-layer chromatography, mouse toxicity, fungistatic effects, and phytotoxic properties. a good correlation was demonstrated between t-2 toxin detection by thin-layer chromatography and inhibition of rhodotorula rubra by culture extracts. all of the strains producing either ...19715575569
[a study of carotenoid pigments of 2 rhodotorula rubra strains]. 19685753520
[yeasts utilizing the surface-active substance laurox-9].yeasts capable of utilizing the non-ionogenic surfactant laurox-9 were identified as cryptococcus humicolus (daszewska) golubev and rhodotorula mucilaginosa (jöerg) harrison. ir spectrometry used for the quantitative assay of laurox-9 showed that r. mucilaginosa utilized it as a sole carbon source. the first step of laurox-9 metabolism was its hydrolytic cleavage yielding lauric acid and polyethyleneglycol. optimal conditions were found for the electron-cytochemical localization of hydrolase inv ...19883419368
a new type of methylamine oxidase: the sole oxidase produced during growth of sporobolomyces albo-rubescens on primary alkylamines.under conditions known to separate methylamine oxidase from benzylamine oxidase in other yeast strains, only a single oxidase could be detected in sporobolomyces albo-rubescens. this occurred irrespective of whether methylamine or n-butylamine was the nitrogen source for growth. the oxidase did not attack benzylamine. it was concluded that this organism can only produce a methylamine oxidase. the enzyme was purified to 90% homogeneity and found to have properties significantly different from the ...19863505743
[study on the presence of yeasts in the feces of the rock pigeon (columba livia gmelin 1789) from rural areas].the diffusion of rock-pigeon (domestic form of columba livia gmelin 1789) is greatly increasing owing to its high reproductivity; for this reason it is present in both countries and cities. this bird is considered an important vector of pathogens to man and domestic animals, and today it represents a serious hygienic-sanitary problem. therefore we checked for the presence of yeasts in feces of rock-pigeons nesting in the regional park "la mandria" (near turin), visited mostly by school-children ...19853870648
[lytic activity against yeasts in bacillus brevis culture broth].the cultural broth of bacillus brevis, strain 5/4, is capable of lysing the cell walls of rhodotorula rubra, candida utilis and other yeasts. the lytic action of the cultural broth is ascribed to proteolysis.19853900650
microbial models of mammalian metabolism: stereospecificity of ketone reduction with pentoxifylline.the absolute configuration of pentoxifylline alcohol produced by the microbial reduction of pentoxifylline using rhodotorula rubra (atcc 20129) was determined by o.r.d. spectroscopy and p.m.r. studies on the r-(+)-alpha-methoxy-alpha-(trifluoromethyl) phenylacetate (mtpa) ester. the development of a reverse-phase h.p.l.c. method for resolving the diastereomeric r-(+)-mtpa esters of racemic pentoxifylline alcohol and assignment of the elution order, allowed for the determination of the stereochem ...19853911603
the mechanism and specificity of iron transport in rhodotorula pilimanae probed by synthetic analogs of rhodotorulic acid.the yeast rhodotorula pilimanae produces the dihydroxamate siderophore rhodotorulic acid (ra) in prodigious amounts when starved for iron. synthetic dihydroxamate analogs of ra have been prepared in which the diketopiperazine ring of ra is replaced by a simple chain of n methylene groups. it is found that r. pilimanae is able to accumulate iron using these achiral complexes, as well as from simple monohydroxamate analogs, at rates comparable to those of ra. while the fe2ra3 complex does not ente ...19854055765
stereospecificity of siderophore-mediated iron uptake in rhodotorula pilimanae as probed by enantiorhodotorulic acid and isomers of chromic rhodotorulate.rhodotorulic acid (ra), a dihydroxamate siderophore produced by rhodotorula pilimanae, forms 3:2 complexes with ferric and chromic ions (m2ra3) at ph 7. kinetically inert chromic complexes of ra have been separated into geometrical isomers and for the first time partially resolved into optical isomers. the three isomers delta-cis, delta-trans, and lambda-trans were characterized by their visible and circular dichroism spectra. inhibition by both delta-isomers of radiolabeled ferric ra uptake in ...19854055766
regulation of aromatic metabolism in fungi: selection of mutants of the yeast rhodotorula mucilaginosa with nystatin. 19744474355
regulation of aromatic metabolism in the fungi: metabolic control of the 3-oxoadipate pathway in the yeast rhodotorula mucilaginosa. 19744474356
[regulation of urease synthesis in rhodotorula mucilaginosa]. 19724672420
dissemination of yeasts after gastrointestinal inoculation in antibiotic-treated mice.mice pretreated with antibiotics were inoculated intragastrically with different yeast isolates to determine whether the resulting disruption of the normal flora ecology would allow certain fungi to colonize and disseminate from the gastrointestinal tract. antibiotic treatment decreased the total population levels of the indigenous bacterial flora, and predisposed mice to gastrointestinal overgrowth and subsequent dissemination by candida albicans, c. parapsilosis, c. pseudotropicalis, c. tropic ...19836342174
serological differences between the multiple amine oxidases of yeasts and comparison of the specificities of the purified enzymes from candida utilis and pichia pastoris.1. antiserum to purified methylamine oxidase of candida boidinii formed precipitin lines (with spurs) in double-diffusion tests with crude extracts of methylamine-grown cells of the following yeast species: candida nagoyaensis, candida nemodendra, hansenula minuta, hansenula polymorpha and pichia pinus. no cross-reaction was observed with extracts of candida lipolytica, candida steatolytica, candida tropicalis, candida utilis, pichia pastoris, sporobolomyces albo-rubescens, sporopachydermia cere ...19836409096
[effect of the genetically active and carcinogenic agent 1-nitroso-imidazolidone-2 on the fine structure of the yeast rhodotorula rubra]. 19685693445
[the biosynthesis of acyclic carotenoids in a mutant of rhodotorula mucilaginosa]. 19676069106
production of egg yolk coloring material by a fermentation process.rhodotorula mucilaginosa was cultivated in a yeast propagator, for the production of biomass and carotenoids. a spray-dried preparation of the yeast was incorporated into the diets of laying chickens for the promotion of egg yolk color. several aspects of the effect of media on growth and carotenogenesis of pigmented yeast were examined. addition of 1 to 2% of rhodotorula yeast to the feeding mixture considerably improved egg yolk color. the possibility of using carotenogenic yeasts as a feed su ...19655893694
a rapid and sensitive paper electrophoresis assay for the detection of microbial siderophores elicited in solid-plating culture.a rapid and sensitive assay for the detection of microbial siderophores (iron-binding compounds) is described. nine representative fungal and bacterial cultures including ustilago sphaerogena, penicillium sp., fusarium roseum, rhodotorula pilimanae, bacillus subtilis w 23, bacillus subtilis w 168, bacillus megaterium, azotobacter vinelandii op, and escherichia coli b, were nutritionally stressed for iron by sequential transfers on iron-deficient solid-plating media. in response to fe-stress cond ...19836614486
mycoflora of the human dermal surfaces.the mycotic flora of the scalp and interdigital areas of the hand and foot of 1296 apparently healthy human inhabitants of three amazonian communities were surveyed by means of microscopic examination of epidermal scrapings and cultural isolation on mycosel agar. no macroscopic or microscopic evidence of fungal infection was detected in any of our study subjects. from 133 (10%) individuals, 143 fungi representing 13 genera and 39 species were recovered. yeasts constituted 85% of the fungi. seven ...19846509388
ecology of pathogenic yeasts in amazonian soil.in an investigation of amazonian soil as a natural reservoir for pathogenic fungi, 1,949 soil samples collected from diverse geographical and ecological settings of the brazilian amazon basin were analyzed for the presence of non-keratinophilic fungi by the indirect mouse inoculation procedure and for the presence of keratinophilic fungi by the hair bait technique. all soil samples were acidic with low ph values. from 12% of the soil samples, 241 yeast and yeastlike isolates pertaining to six ge ...19846538774
characterization of a novel l-asparaginase produced by rhodotorula rubra. 19807027389
[persistent localized hyperkeratosis caused by rhodotorula rubra (author's transl)]. 19827075845
yeasts in the intertidal sediments of a polluted estuary in rio de janeiro, brazil.yeasts were found concentrated at the sediment surface and with the highest counts at the most polluted site. candida krusei, pichia membranaefaciens and similar species typically forming rugose colonies with radiating ridges were the prevalent yeasts in these sediments, and species like rhodotorula rubra related to basidiomycetous fungi were found in relatively low numbers.19827092202
rhodotorula rubra peritonitis in patients undergoing continuous ambulatory peritoneal dialysis.although still uncommon, fungal peritonitis is being reported with increasing frequency in patients with renal failure who are undergoing long-term peritoneal dialysis. although most cases have involved candida albicans, other opportunistic pathogens have been reported as well. recently, three patients were seen with serious peritoneal infections due to rhodotorula rubra, a "nonpathogenic" fungus. in two patients, peritoneal fibrosis made further peritoneal dialysis impossible. the third patient ...19836881189
dissimilation of aromatic compounds in rhodotorula graminis: biochemical characterization of pleiotropically negative mutants.microorganisms oxidize many aromatic compounds through the dihydroxylated intermediates catechol and protocatechuate and through the beta-ketoadipate pathway. the catabolic sequences used by the yeast rhodotorula graminis for the dissimilation of aromatic compounds were elucidated after biochemical analysis of pleiotropically negative mutant strains. growth properties of one mutant strain revealed that benzoate-4-hydroxylase was required for the utilization of phenylalanine, mandelate, and benzo ...19846542098
ovine pulmonary infection caused by rhodotorula rubra. 19807402216
sensitivity of heat-stressed yeasts to essential oils of plants.eight strains of yeasts (candida lipolytica, debaryomyces hansenii, hansenula anomala, kloeckera apiculata, lodderomyces elongisporus, rhodotorula rubra, saccharomyces cerevisiae, and torulopsis glabrata) were examined for changes in sensitivity to eight essential oils of plants (allspice, cinnamon, clove, garlic, onion, oregano, savory, and thyme) after being sublethally heat stressed. with the exception of garlic oil for all test yeasts, onion oil for s. cerevisiae, and oregano oil for r. rubr ...19846712207
rdna targeted oligonucleotide primers for the identification of pathogenic yeasts in a polymerase chain reaction.species-specific oligonucleotide primers were designed for pcr identification of the basidiomycetous yeasts cryptococcus neoformans, trichosporon cutaneum and rhodotorula mucilaginosa. the procedure uses standard pcr components including dna from the test species and three primers: two universal external (upstream and downstream) limiting primers and a species-specific internal primer. species identification requires the formation of a species-specific rdna nucleotide segment that is significant ...19957662289
rhodotorula rubra peritonitis in an hiv+ patient on capd. 19957705519
response of bacteria and fungi to high-pressure stress as investigated by two-dimensional polyacrylamide gel electrophoresis.in an attempt to generalize previous observations (jaenicke et al., appl. environ. microbiol. 1988, 54, 2375-2380) and to find a convenient model system for studies of the pressure response, we tested the suitability of escherichia coli and thermotoga maritima (bacteria), and of five different eukaryotic species including the filamentous fungi asteromyces cruciatus and dendryphiella salina, and the marine yeasts debaryomyces hansenii, rhodosporidium sphaerocarpum, and rhodotorula rubra. using tw ...19947720693
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