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comparison of fusarium acuminatum and fusarium culmorum isolates by means of tandem-crossed immunoelectrophoresis.f. acuminatum and f. culmorum strains were compared by means of tandem-crossed immunoelectrophoresis in order to estimate the possibilities of serological classification in fusarium sections "gibbosum" and "discolor". on the basis of qualitative similarity the two species could be distinguished well. by the use of anti-f. acuminatum serum a similarity of ssm = 0.52 was found between f. acuminatum and f. culmorum, but the ssm coefficient reached a value of 0.67 when the anti-f. culmorum serum was ...1979582892
comparison of fusarium acuminatum and fusarium culmorum isolates by means of tandem-crossed immunoelectrophoresis.f. acuminatum and f. culmorum strains were compared by means of tandem-crossed immunoelectrophoresis in order to estimate the possibilities of serological classification in fusarium sections "gibbosum" and "discolor". on the basis of qualitative similarity the two species could be distinguished well. by the use of anti-f. acuminatum serum a similarity of ssm = 0.52 was found between f. acuminatum and f. culmorum, but the ssm coefficient reached a value of 0.67 when the anti-f. culmorum serum was ...1979582892
effects of mbc on hyphal tip organization, growth, and mitosis of fusarium acuminatum, and their antagonism by d2o. 1977910080
effects of mbc on hyphal tip organization, growth, and mitosis of fusarium acuminatum, and their antagonism by d2o. 1977910080
t-2 toxin production by fusarium acuminatum isolated from oats and barley.oats grain from south africa was frequently found to be infested by toxic strains of fusarium acuminatum, as was one barley sample. all 11 toxic strains tested produced t-2 toxin (0.8 to 2,600 mg/kg), and 6 of 11 strains produced diacetoxyscirpenol (0.6 to 8.4 mg/kg). this is the first record of t-2 toxin-producing fusarium isolates from africa and of the production of large amounts of t-2 toxin at relatively high (25 degrees c) temperatures.19863767364
t-2 toxin production by fusarium acuminatum isolated from oats and barley.oats grain from south africa was frequently found to be infested by toxic strains of fusarium acuminatum, as was one barley sample. all 11 toxic strains tested produced t-2 toxin (0.8 to 2,600 mg/kg), and 6 of 11 strains produced diacetoxyscirpenol (0.6 to 8.4 mg/kg). this is the first record of t-2 toxin-producing fusarium isolates from africa and of the production of large amounts of t-2 toxin at relatively high (25 degrees c) temperatures.19863767364
stimulation of aflatoxin b1 and t-2 toxin production by sorbic acid.aspergillus flavus grown on yeast extract-sucrose medium produced higher amounts of aflatoxin b1 in the presence of 0.025% sorbic acid than without this chemical with a maximum at 17 days of incubation. addition of 0.05 to 0.0125% sorbic acid stimulated t-2 toxin production of fusarium acuminatum cultures grown on maize meal. the highest amounts of the mycotoxin were detected in 14-day-old cultures containing 0.025% sorbic acid. it is assumed that certain amounts of sorbic acid near the minimal ...19846424567
stimulation of aflatoxin b1 and t-2 toxin production by sorbic acid.aspergillus flavus grown on yeast extract-sucrose medium produced higher amounts of aflatoxin b1 in the presence of 0.025% sorbic acid than without this chemical with a maximum at 17 days of incubation. addition of 0.05 to 0.0125% sorbic acid stimulated t-2 toxin production of fusarium acuminatum cultures grown on maize meal. the highest amounts of the mycotoxin were detected in 14-day-old cultures containing 0.025% sorbic acid. it is assumed that certain amounts of sorbic acid near the minimal ...19846424567
toxicity and trichothecene production by fusarium acuminatum subsp. acuminatum and fusarium acuminatum subsp. armeniacum.the toxicity of cultures of fusarium acuminatum subsp. acuminatum and fusarium acuminatum subsp. armeniacum grown on weet-bix medium was assessed using a chick bioassay. thirty-nine of 45 cultures of f. a. armeniacum tested produced at least 50% mortality in the chick bioassay. in contrast, of the 26 cultures of f. a. acuminatum tested, only nine produced at least 50% mortality. selected extracts of both subspecies were analyzed by gas chromatography after clean-up and hydrolysis for the four ma ...19938167939
toxicity and trichothecene production by fusarium acuminatum subsp. acuminatum and fusarium acuminatum subsp. armeniacum.the toxicity of cultures of fusarium acuminatum subsp. acuminatum and fusarium acuminatum subsp. armeniacum grown on weet-bix medium was assessed using a chick bioassay. thirty-nine of 45 cultures of f. a. armeniacum tested produced at least 50% mortality in the chick bioassay. in contrast, of the 26 cultures of f. a. acuminatum tested, only nine produced at least 50% mortality. selected extracts of both subspecies were analyzed by gas chromatography after clean-up and hydrolysis for the four ma ...19938167939
beauvericin production by fusarium species.beauvericin is a cyclohexadepsipeptide mycotoxin which has insecticidal properties and which can induce apoptosis in mammalian cells. beauvericin is produced by some entomo- and phytopathogenic fusarium species (fusarium proliferatum, f. semitectum, and f. subglutinans) and occurs naturally on corn and corn-based foods and feeds infected by fusarium spp. we tested 94 fusarium isolates belonging to 25 taxa, 21 in 6 of the 12 sections of the fusarium genus and 4 that have been described recently, ...19989687479
beauvericin production by fusarium species.beauvericin is a cyclohexadepsipeptide mycotoxin which has insecticidal properties and which can induce apoptosis in mammalian cells. beauvericin is produced by some entomo- and phytopathogenic fusarium species (fusarium proliferatum, f. semitectum, and f. subglutinans) and occurs naturally on corn and corn-based foods and feeds infected by fusarium spp. we tested 94 fusarium isolates belonging to 25 taxa, 21 in 6 of the 12 sections of the fusarium genus and 4 that have been described recently, ...19989687479
fusarium species from nepalese rice and production of mycotoxins and gibberellic acid by selected species.infection of cereal grains with fusarium species can cause contamination with mycotoxins that affect human and animal health. to determine the potential for mycotoxin contamination, we isolated fusarium species from samples of rice seeds that were collected in 1997 on farms in the foothills of the nepal himalaya. the predominant fusarium species in surface-disinfested seeds with husks were species of the gibberella fujikuroi complex, including g. fujikuroi mating population a (anamorph, fusarium ...200010698766
fusarium species from nepalese rice and production of mycotoxins and gibberellic acid by selected species.infection of cereal grains with fusarium species can cause contamination with mycotoxins that affect human and animal health. to determine the potential for mycotoxin contamination, we isolated fusarium species from samples of rice seeds that were collected in 1997 on farms in the foothills of the nepal himalaya. the predominant fusarium species in surface-disinfested seeds with husks were species of the gibberella fujikuroi complex, including g. fujikuroi mating population a (anamorph, fusarium ...200010698766
sensitivity of partially purified ice nucleation activity of fusarium acuminatum srsf 616.factors that affect bacterial ice nucleation, including growth medium, growth phase, nutrient deprivation, and cold-temperature exposure, were investigated in the ice nucleation active (ina) fungus fusarium acuminatum srsf 616. ice nucleation activity remained relatively constant throughout the growth cycle, and the cell-free culture supernatant consistently displayed higher ice nucleation activity than the hyphal pellet. although nutrient starvation and low-temperature exposure enhance bacteria ...200111400053
sensitivity of partially purified ice nucleation activity of fusarium acuminatum srsf 616.factors that affect bacterial ice nucleation, including growth medium, growth phase, nutrient deprivation, and cold-temperature exposure, were investigated in the ice nucleation active (ina) fungus fusarium acuminatum srsf 616. ice nucleation activity remained relatively constant throughout the growth cycle, and the cell-free culture supernatant consistently displayed higher ice nucleation activity than the hyphal pellet. although nutrient starvation and low-temperature exposure enhance bacteria ...200111400053
intracellular superoxide dismutase, catalase, and glutathione peroxidase activities and membrane lipid peroxide levels in fusarium acuminatum upon environmental changes in a defined medium.the variations of superoxide dismutase (sod), catalase (cat), and glutathione peroxidase (gsh-px) activities and lipid peroxide (lpo) levels in fusarium acuminatum, an aerobic filamentous fungus, were investigated depending on the carbon and nitrogen sources during the incubation period. fungus was cultivated in growing medium containing either maltose or saccharose in 5-25 g/l concentration range as a carbon source and either glycine or peptone in 5-35 g/l concentration range as a nitrogen sour ...200212054437
intracellular superoxide dismutase, catalase, and glutathione peroxidase activities and membrane lipid peroxide levels in fusarium acuminatum upon environmental changes in a defined medium.the variations of superoxide dismutase (sod), catalase (cat), and glutathione peroxidase (gsh-px) activities and lipid peroxide (lpo) levels in fusarium acuminatum, an aerobic filamentous fungus, were investigated depending on the carbon and nitrogen sources during the incubation period. fungus was cultivated in growing medium containing either maltose or saccharose in 5-25 g/l concentration range as a carbon source and either glycine or peptone in 5-35 g/l concentration range as a nitrogen sour ...200212054437
species-specific pcr-based assays for the detection of fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis.species-specific pcr was used for the identification of nine fusarium species in pure mycelial culture. a pcr-based method was compared with the whole seed agar plate method and trichothecene analysis for three toxin-producing fusarium species using 85 grain samples of wheat, barley, oat, corn and rye. a simple sds-based dna extraction system followed by potassium acetate precipitation resulted in consistent pcr amplification of dna fragments from cultures and grain samples. the species-specific ...200516099312
species-specific pcr-based assays for the detection of fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis.species-specific pcr was used for the identification of nine fusarium species in pure mycelial culture. a pcr-based method was compared with the whole seed agar plate method and trichothecene analysis for three toxin-producing fusarium species using 85 grain samples of wheat, barley, oat, corn and rye. a simple sds-based dna extraction system followed by potassium acetate precipitation resulted in consistent pcr amplification of dna fragments from cultures and grain samples. the species-specific ...200516099312
ice nucleation activity in fusarium acuminatum and fusarium avenaceum.twenty fungal genera, including 14 fusarium species, were examined for ice nucleation activity at -5.0 degrees c, and this activity was found only in fusarium acuminatum and fusarium avenaceum. this characteristic is unique to these two species. ice nucleation activity of f. avenaceum was compared with ice nucleation activity of a pseudomonas sp. strain. cumulative nucleus spectra are similar for both microorganisms, while the maximum temperatures of ice nucleation were -2.5 degrees c for f. ave ...199216348770
ice nucleation activity in fusarium acuminatum and fusarium avenaceum.twenty fungal genera, including 14 fusarium species, were examined for ice nucleation activity at -5.0 degrees c, and this activity was found only in fusarium acuminatum and fusarium avenaceum. this characteristic is unique to these two species. ice nucleation activity of f. avenaceum was compared with ice nucleation activity of a pseudomonas sp. strain. cumulative nucleus spectra are similar for both microorganisms, while the maximum temperatures of ice nucleation were -2.5 degrees c for f. ave ...199216348770
stimulation of defense reactions in medicago truncatula by antagonistic lipopeptides from paenibacillus sp. strain b2.with the aim of obtaining new strategies to control plant diseases, we investigated the ability of antagonistic lipopolypeptides (paenimyxin) from paenibacillus sp. strain b2 to elicit hydrogen peroxide (h₂o₂) production and several defense-related genes in the model legume medicago truncatula. for this purpose, m. truncatula cell suspensions were used and a pathosystem between m. truncatula and fusarium acuminatum was established. in m. truncatula cell cultures, the induction of h₂o₂ reached a ...201020870792
stimulation of defense reactions in medicago truncatula by antagonistic lipopeptides from paenibacillus sp. strain b2.with the aim of obtaining new strategies to control plant diseases, we investigated the ability of antagonistic lipopolypeptides (paenimyxin) from paenibacillus sp. strain b2 to elicit hydrogen peroxide (h₂o₂) production and several defense-related genes in the model legume medicago truncatula. for this purpose, m. truncatula cell suspensions were used and a pathosystem between m. truncatula and fusarium acuminatum was established. in m. truncatula cell cultures, the induction of h₂o₂ reached a ...201020870792
identification of new galactose oxidase genes in fusarium spp.galactose oxidase (go) converts galactose to an aldehyde and has several biotechnological applications, including cancer diagnosis. it is mainly produced by fusarium austroamericanum but is also produced by fusarium acuminatum and by isolates of the fusarium graminearum and gibberella fujikuroi complexes. the f. austroamericanum go gaoa gene has been cloned, but the go genes from other secreting species have not been characterized. problems associated with the f. austroamericanum go such as high ...201021077113
identification of new galactose oxidase genes in fusarium spp.galactose oxidase (go) converts galactose to an aldehyde and has several biotechnological applications, including cancer diagnosis. it is mainly produced by fusarium austroamericanum but is also produced by fusarium acuminatum and by isolates of the fusarium graminearum and gibberella fujikuroi complexes. the f. austroamericanum go gaoa gene has been cloned, but the go genes from other secreting species have not been characterized. problems associated with the f. austroamericanum go such as high ...201021077113
identification and characterization of ethanol utilizing fungal flora of oil refinery contaminated soil.the indigenous fungal flora of three oil refinery contaminated sites (bharuch, valsad and vadodara) of india has been documented in the present investigation. a total seventy-five fungal morphotypes were isolated from these sites and out of them, only fifteen isolates were capable of utilizing ethanol (0-8%; v:v) as a sole source of carbon and energy for growth. ten percent ethanol was completely lethal for the growth of all the isolated fungus. biochemical characterization of the potent ethanol ...201424068535
wildly growing asparagus (asparagus officinalis l.) hosts pathogenic fusarium species and accumulates their mycotoxins.asparagus officinalis l. is an important crop in many european countries, likely infected by a number of fusarium species. most of them produce mycotoxins in plant tissues, thus affecting the physiology of the host plant. however, there is lack of information on fusarium communities in wild asparagus, where they would definitely have considerable environmental significance. therefore, the main scientific aim of this study was to identify the fusarium species and quantify their typical mycotoxins ...201626687343
atp citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the fusarium tricinctum species complex in pure cultures and in cereal samples.the combined data set of the acl1 and tef-1α gene sequences of 61 fungal strains assigned to fusarium tricinctum, fusarium avenaceum, fusarium acuminatum, fusarium arthrosporioides, fusarium flocciferum and fusarium torulosum were used to study the phylogenetic relations between taxa. f. tricinctum, f. acuminatum and f. avenaceum formed distinct clades. members of the f. tricinctum/f. acuminatum clade fall into three well supported lineages, of which the largest includes the epitype of f. tricin ...201222867849
[fusarium species associated with basal rot of garlic in north central mexico and its pathogenicity].garlic in mexico is one of the most profitable vegetable crops, grown in almost 5,451ha; out of which more than 83% are located in zacatecas, guanajuato, sonora, puebla, baja california and aguascalientes. blossom-end rot caused by fusarium spp is widely distributed worldwide and has been a limiting factor in onion and garlic production regions, not only in mexico but also in other countries. the presence of fusarium oxysporum has been reported in guanajuato and aguascalientes. fusarium culmorum ...201727590608
phylogenetic analyses and toxigenic profiles of fusarium equiseti and fusarium acuminatum isolated from cereals from southern europe.fusarium equiseti and fusarium acuminatum are toxigenic species that contaminate cereal crops from diverse climatic regions. they are common in spanish cereals. the information available on their phylogenetics and toxigenic profiles is, however, insufficient to assist risk evaluation. in this work, phylogenetic analyses were performed using partial sequences of the translation elongation factor gene (ef-1α) of f. equiseti and f. acuminatum strains isolated from barley and wheat from spain and ot ...201222608228
a comparative study of the metal ion uptake and antioxidant enzyme activities of fusarium equiseti and fusarium acuminatum as a function of external magnesium concentration.the increase of mg2+, from 1.3 to 3 microm, in growth medium of f. equiseti and f. acuminatum increased intracellular magnesium levels from 0.83 and 0.81 microm to 1.75 and 1.42 microm on the 12th day, respectively. intracellular magnesium levels also elevated depending upon the number of incubation days. the maximum manganese levels of f. equiseti and f. acuminatum obtained in 1.6 microm mg2+ culture medium were 0.67 and 1.23 microm, while maximum iron levels were determined to be 1.3 microm mg ...200516109634
a comparative study of the metal ion uptake and antioxidant enzyme activities of fusarium equiseti and fusarium acuminatum as a function of external magnesium concentration.the increase of mg2+, from 1.3 to 3 microm, in growth medium of f. equiseti and f. acuminatum increased intracellular magnesium levels from 0.83 and 0.81 microm to 1.75 and 1.42 microm on the 12th day, respectively. intracellular magnesium levels also elevated depending upon the number of incubation days. the maximum manganese levels of f. equiseti and f. acuminatum obtained in 1.6 microm mg2+ culture medium were 0.67 and 1.23 microm, while maximum iron levels were determined to be 1.3 microm mg ...200516109634
cytoplasmic microtubules and fungal morphogenesis: ultrastructural effects of methyl benzimidazole-2-ylcarbamate determined by freeze-substitution of hyphal tip cells.the effects of methyl benzimidazole-2-ylcarbamate (mbc), one of only a few agents that are active against microtubules of fungi, were analyzed at the ultrastructural level in freeze-substituted hyphal tip cells of fusarium acuminatum. nontreated and control cells had numerous microtubules throughout. after just 10 min of exposure to mbc, almost no cytoplasmic microtubules were present, except near spindle pole bodies. after 45 min of exposure to mbc, no microtubules were present in hyphal tip ce ...19807419600
cytoplasmic microtubules and fungal morphogenesis: ultrastructural effects of methyl benzimidazole-2-ylcarbamate determined by freeze-substitution of hyphal tip cells.the effects of methyl benzimidazole-2-ylcarbamate (mbc), one of only a few agents that are active against microtubules of fungi, were analyzed at the ultrastructural level in freeze-substituted hyphal tip cells of fusarium acuminatum. nontreated and control cells had numerous microtubules throughout. after just 10 min of exposure to mbc, almost no cytoplasmic microtubules were present, except near spindle pole bodies. after 45 min of exposure to mbc, no microtubules were present in hyphal tip ce ...19807419600
ultrastructural analysis of hyphal tip cell growth in fungi: spitzenkörper, cytoskeleton and endomembranes after freeze-substitution.the ultrastructure of freeze-substituted tip cells of fusarium acuminatum was analysed by conventional and high-voltage transmission electron microscopy (hvem). at least 2 morphologically distinct types of golgi-like endomembrane cisternae were observed, each existing as single, fenestrated sheets and tubular elements that were often very closely associated with mitochondria. from hvem observations of thick (0.25 and 0.5 micron) sections, the spitzenkörper appeared to correspond to an apical mas ...19817196918
ultrastructural analysis of hyphal tip cell growth in fungi: spitzenkörper, cytoskeleton and endomembranes after freeze-substitution.the ultrastructure of freeze-substituted tip cells of fusarium acuminatum was analysed by conventional and high-voltage transmission electron microscopy (hvem). at least 2 morphologically distinct types of golgi-like endomembrane cisternae were observed, each existing as single, fenestrated sheets and tubular elements that were often very closely associated with mitochondria. from hvem observations of thick (0.25 and 0.5 micron) sections, the spitzenkörper appeared to correspond to an apical mas ...19817196918
improvement of the biodegradation of some cellulosic wastes by acid pretreatment.acid pretreatment of cellulosic wastes improved their susceptibility to fusarium acuminatum enzymes. the effectiveness of acid pretreatment was demonstrated with an increase in both fungal growth and enzyme activities. a growth yield of 0.15 g/100 ml was achieved on medium containing 5% acid pretreated pods of bean for 60 minutes. avicelase (c1), carboxymethylcellulase (cx) and b-glucosidase (c2) reached their maximal biosynthesis on acid pretreated wheat bran, sugar-cane bagasse and sawdust-con ...19911726620
improvement of the biodegradation of some cellulosic wastes by acid pretreatment.acid pretreatment of cellulosic wastes improved their susceptibility to fusarium acuminatum enzymes. the effectiveness of acid pretreatment was demonstrated with an increase in both fungal growth and enzyme activities. a growth yield of 0.15 g/100 ml was achieved on medium containing 5% acid pretreated pods of bean for 60 minutes. avicelase (c1), carboxymethylcellulase (cx) and b-glucosidase (c2) reached their maximal biosynthesis on acid pretreated wheat bran, sugar-cane bagasse and sawdust-con ...19911726620
swelling of penicillium digitatum conidia by a fusarium acuminatum nrrl 6227 metabolite.fusarium acuminatum nrrl 6227 produces an antifungal metabolite that causes incubating conidia of several penicillium species to swell 5-10 diameters while inhibiting germination. the swollen conidia are spherical, translucent, nonviable and easily shattered with a slight physical pressure; however, they remain resistant to osmotic shock. this antibiotic has been identified as a cyclic peptide composed of alanine, glutamic acid, leucine, threonine and tyrosine.1977593300
swelling of penicillium digitatum conidia by a fusarium acuminatum nrrl 6227 metabolite.fusarium acuminatum nrrl 6227 produces an antifungal metabolite that causes incubating conidia of several penicillium species to swell 5-10 diameters while inhibiting germination. the swollen conidia are spherical, translucent, nonviable and easily shattered with a slight physical pressure; however, they remain resistant to osmotic shock. this antibiotic has been identified as a cyclic peptide composed of alanine, glutamic acid, leucine, threonine and tyrosine.1977593300
toxigenicity of enniatins from western australian fusarium species to brine shrimp (artemia franciscana).the high prevalence (14 of 24 isolates) of enniatin-producing isolates from western australian fusarium species isolated from pasture legumes associated with sheep feed refusal and rat deaths, and the high toxicity of their crude extracts to brine shrimp (artemia franciscana) from a previous study warranted further investigation of this class of mycotoxin. crude extracts from fusarium acuminatum, fusarium avenaceum, fusarium tricinctum and fusarium sambucinum, along with enniatins a, a1, b and b ...201121352844
toxigenicity of enniatins from western australian fusarium species to brine shrimp (artemia franciscana).the high prevalence (14 of 24 isolates) of enniatin-producing isolates from western australian fusarium species isolated from pasture legumes associated with sheep feed refusal and rat deaths, and the high toxicity of their crude extracts to brine shrimp (artemia franciscana) from a previous study warranted further investigation of this class of mycotoxin. crude extracts from fusarium acuminatum, fusarium avenaceum, fusarium tricinctum and fusarium sambucinum, along with enniatins a, a1, b and b ...201121352844
structural studies on minor enniatins from fusarium sp. vi 03441: novel n-methyl-threonine containing enniatins.a strain of a yet unidentified fusarium sp. produced in addition to enniatins a1, b and b1 a number of minor enniatins. the strain formed a well supported clade with strains identified as fusarium acuminatum (gibberella acuminata) in phylogenetic analyses using the tef-1alpha gene sequences. two of the minor enniatins were easily recognised as hydroxylated species on the basis of their fragment ion spectra. the hydroxylation could be traced to one of the amino acid moieties using multiple-stage ...200919249325
structural studies on minor enniatins from fusarium sp. vi 03441: novel n-methyl-threonine containing enniatins.a strain of a yet unidentified fusarium sp. produced in addition to enniatins a1, b and b1 a number of minor enniatins. the strain formed a well supported clade with strains identified as fusarium acuminatum (gibberella acuminata) in phylogenetic analyses using the tef-1alpha gene sequences. two of the minor enniatins were easily recognised as hydroxylated species on the basis of their fragment ion spectra. the hydroxylation could be traced to one of the amino acid moieties using multiple-stage ...200919249325
interactions of neotyphodium gansuense, achnatherum inebrians, and plant-pathogenic fungi.interactions of neotyphodium gansuense, achnatherum inebrians, and nine fungal pathogens were studied by tests of inhibition of four fungal pathogens by neotyphodium endophytes in vitro and by inoculation of nine fungal pathogens on detached leaves of endophyte-infected (e+) and endophyte-free (e-) plants. compared with the controls, most isolates of n. gansuense significantly inhibited the growth in vitro of, in decreasing order of inhibition, bipolaris sorokiniana, curvularia lunata, fusarium ...200717988846
interactions of neotyphodium gansuense, achnatherum inebrians, and plant-pathogenic fungi.interactions of neotyphodium gansuense, achnatherum inebrians, and nine fungal pathogens were studied by tests of inhibition of four fungal pathogens by neotyphodium endophytes in vitro and by inoculation of nine fungal pathogens on detached leaves of endophyte-infected (e+) and endophyte-free (e-) plants. compared with the controls, most isolates of n. gansuense significantly inhibited the growth in vitro of, in decreasing order of inhibition, bipolaris sorokiniana, curvularia lunata, fusarium ...200717988846
production, purification, and characterization of a novel galactose oxidase from fusarium acuminatum.extra-cellular production of a novel galactose oxidase from fusarium acuminatum using submerged fermentation was studied. glucose (1.0% w/v) was used as the sole carbon source. maximum galactose oxidase production (approximately 4.0 u/ml) was obtained when fermentation was carried out at 25 degrees c, with orbital shaking (100 rpm) and an initial medium of ph 7.0, for 96 h, using a 2% (v/v) inoculum made from a homogenized four-day-old liquid culture, in the presence of copper, manganese, and ma ...200717518413
production, purification, and characterization of a novel galactose oxidase from fusarium acuminatum.extra-cellular production of a novel galactose oxidase from fusarium acuminatum using submerged fermentation was studied. glucose (1.0% w/v) was used as the sole carbon source. maximum galactose oxidase production (approximately 4.0 u/ml) was obtained when fermentation was carried out at 25 degrees c, with orbital shaking (100 rpm) and an initial medium of ph 7.0, for 96 h, using a 2% (v/v) inoculum made from a homogenized four-day-old liquid culture, in the presence of copper, manganese, and ma ...200717518413
inoculation of astragalus racemosus and astragalus convallarius with selenium-hyperaccumulator rhizosphere fungi affects growth and selenium accumulation.little is known about how fungi affect plant selenium (se) accumulation. here we investigate the effects of two fungi on se accumulation, translocation, and chemical speciation in the hyperaccumulator astragalus racemosus and the non-accumulator astragalus convallarius. the fungi, alternaria astragali (a3) and fusarium acuminatum (f30), were previously isolated from astragalus hyperaccumulator rhizosphere. a3-inoculation enhanced growth of a. racemosus yet inhibited growth of a. convallarius. se ...201323117393
fungus-mediated synthesis of gold nanoparticles and standardization of parameters for its biosynthesis.we report the extracellular biosynthesis of gold nanoparticles (aunps) using a fungus fusarium acuminatum. mycosynthesis of au-nps was carried out by challenging the fungal cells filtrate with haucl 4 solution (1 mm), as nanoparticles synthesizing enzyme secrete extracellularly by the fungi. the aunps were characterized with the help of uv-visible spectrophotometer, fourier transform infrared spectroscopy, zeta potential, x-ray diffraction (xrd) and transmission electron microscopy (tem). we obs ...201425163069
diversity of toxic and phytopathogenic fusarium species occurring on cereals grown in karnataka state, india.a total of 198 cereal samples (53 maize, 54 sorghum, 37 paddy and 54 wheat) were collected from 11 districts of karnataka to understand the percent infection (pi), relative density (rd) and their frequency (fr) caused by fusarium spp. all samples were screened by agar plating method using mga 2.5 agar media and incubated at 25 ± 2 °c for 3-5 days. the study revealed the association of 10 different fusarium species known trichothecene producers such as fusarium acuminatum, f. avenaceum, f. crookw ...028330127
genome sequences of six wheat-infecting fusarium species isolates.fusarium pathogens represent a major constraint to wheat and barley production worldwide. to facilitate future comparative studies of fusarium species that are pathogenic to wheat, the genome sequences of four fusarium pseudograminearum isolates, a single fusarium acuminatum isolate, and an organism from the fusarium incarnatum-f. equiseti species complex are reported.201324009115
an ice nucleation protein from fusarium acuminatum: cloning, expression, biochemical characterization and computational modeling.ice nucleation proteins (inp) are a major cause of frost damage in plants and crops. here, an inp gene from fusarium acuminatum was optimized, synthesized, expressed in e.coli and subsequently purified and characterized. the protein belongs to the second class of ice nucleation proteins with an optimum ph 5.5, relative activity and stability between ph 5 and 9.5 and up to 45 °c. the protein was fully active and stable in the presence of dimethyl sulfoxide (dmso), dioxane, acetone and ethyl aceta ...201424930104
role of pyruvate and ascorbate production in regulation of antioxidant enzymes and membrane lpo levels in fusarium acuminatum.the role of pyruvate and ascorbate in the regulation of superoxide dismutase (sod); catalase (cat); glutathione peroxidase enzymes; and, therefore, membrane lipid peroxidation (lpo) levels in fusarium acuminatum was investigated in media containing either glycerin or glucose as a carbon source, depending on the incubation period, in the range of 5-25 g/l. increasing sod activity between d 9 and 16 of the incubation period showed a positive correlation with a significant increase in pyruvate prod ...200515640554
role of pyruvate and ascorbate production in regulation of antioxidant enzymes and membrane lpo levels in fusarium acuminatum.the role of pyruvate and ascorbate in the regulation of superoxide dismutase (sod); catalase (cat); glutathione peroxidase enzymes; and, therefore, membrane lipid peroxidation (lpo) levels in fusarium acuminatum was investigated in media containing either glycerin or glucose as a carbon source, depending on the incubation period, in the range of 5-25 g/l. increasing sod activity between d 9 and 16 of the incubation period showed a positive correlation with a significant increase in pyruvate prod ...200515640554
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