| non-competitive inhibition of rb+ and so4(2-) uptake caused by curvularia lunata phytotoxins. | | 1979 | 36995 |
| the post-harvest fruit rots of tomato (lycopersicum esculentum) in nigeria. | a survey of the post-harvest fruit rot diseases of tomato was conducted in five states of nigeria. during severe infections, the diseases could cause 25% loss at harvest and 34% loss of the remaining product in transit, storage and market stalls; thus giving an overall loss of about 50% of the product. two types of rots, soft and dry were recognised. the soft rot was found to account for about 85% and the dry rot about 15% of the overall loss. erwinia carotovora, rhizopus oryzae, r. stolonifer, ... | 1979 | 471028 |
| effect of nutrient variations on growth and brefeldin a formation in curvularia lunata. | the highest level of secreted brefeldin a was present in glucose-grown cultures, intermediate levels in glucose-fructose, and xylose cultures and low levels in fructose- and galactose-grown cultures of curvularia lunata. the biomass decreased from glucose, fructose, xylose, glucose-fructose to galactose cultures. brefeldin a levels and mycelial yields were low in citrate-, gluconate-, and succinate-grown cultures. inorganic phosphate-limited cultures supported a high level of brefeldin a. interm ... | 1977 | 557020 |
| effect of metabolic inhibitors on growth and brefeldin a formation in curvularia lunata. | arsenite as opposed to azide exerted a stronger inhibitory effect on brefeldin a production than on mycelial growth of cultures of curvularia lunata. however, azide was a more potent inhibitor of mycelial growth than arsenite at 0.5 mm and 1.0 mm. the inhibitory effects of iodoacetate on growth and brefeldin a formation were significantly less potent than those of iosoacetamide. increases in the levels of fluoride elicited a variable inhibitory effect on brefeldin a production and a correspondin ... | 1978 | 564318 |
| effect of brefeldin a and actinomycin d on culture growth and brefeldin a yield in curvularia lunata. | cultures incorporated with increasing quantities of brefeldin a in the form of crude extracts of fungal metabolites prior to inoculation demonstrated reduced growth rate and no significant increase in brefeldin a content. on the other hand, cultures incubated with increasing levels of actinomycin d on the 8th day of cultivation showed slight stimulation of brefeldin a formation with insignificant effect on growth. | 1978 | 565737 |
| effect of malonate and maleate on growth and brefeldin a formation in curvularia lunata. | malonate exerted a stronger inhibitory effect on brefeldin a production than on mycelia growth in cultures of curvularia lunata especially at inhibitory levels of 100 to 200 mm. the extent of 200 mm malonate inhibition of growth and brefeldin a production was greater in cultures treated with malonate prior to inoculation than those treated following 5 days after inoculation. maleate at levels of 40 to 220 mm activated brefeldin a formation in cultures though exerting variable effects on mycelia ... | 1978 | 566696 |
| disseminated curvularia lunata infection in a football player. | for ten years, a 25-year-old immune-competent man experienced a progressive disseminated infection with the saprophytic soil fungus, curvularia lunata, following presumptive cutaneous inoculation while playing football. deep, soft tissue abscesses, pulmonary suppuration, paravertebral abscess, and cerebral abscess all followed leg ulcers from neglected abrasions. the patient's delay in obtaining treatment was partially responsible for the paravertebral-mediastinal-pleural-cutaneous fistula that ... | 1979 | 572662 |
| microbial transformations of natural antitumor agents: conversion of lapachol to dehydro-alpha-lapachone by curvularia lunata. | microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by curvularia lunata (nrrl 2178). the fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its structure was verified by chemical synthesis. the metabolite is a naturally occurring chromene possessing antibacterial and antitumor activities. | 1979 | 574750 |
| 11beta-hydroxylation of progesterone with curvularia lunata. | | 1975 | 1205538 |
| elimination of by-products in 11 beta-hydroxylation of substance s using curvularia lunata clones regenerated from ntg-treated protoplasts. | stable mutants showing improved 11-hydroxylation of substance s were isolated, following treatment with n-methyl-n'-nitro-n-nitrosoguanidine (ntg) and regeneration of uninucleate protoplasts of the appropriate fungal strains. this procedure was especially suitable for obtaining more directed 11 beta-hydroxylation of substance s with curvularia lunata im 2901. apart from producing cortisol (11 beta-hydroxy-s), the parent strain formed several by-products that significantly lowered the yield of th ... | 1992 | 1368917 |
| transformation of cochliobolus lunatus with put 720 changes the steroid hydroxylating ability of the fungus. | the filamentous fungus cochliobolus lunatus, a known 11 beta-hydroxylator of steroids, was transformed to bleomycin resistance using the heterologous plasmid put 720. this plasmid contains the sh ble gene expressed under the control of the aspergillus nidulans gpd and trpc expression signals. the bleomycin-resistant colonies appeared with a frequency of six per microgram of dna. all colonies were real transformants and no "abortive" growth was observed. in all transformants tested the plasmid mo ... | 1992 | 1384995 |
| bioconversion of steroids by cochliobolus lunatus--ii. 11 beta-hydroxylation of 17 alpha, 21-dihydroxypregna-1,4-diene-3,20-dione 17-acetate in dependence of the inducer structure. | the 11 beta-hydroxylase of the filamentous fungus cochliobolus lunatus m 118 was induced with the substrate 17 alpha, 21-dihydroxypregna-1,4-diene-3,20-dione 17-acetate (11 beta-deoxyprednisolone 17-acetate) itself, substrate analogues, different pregnane compounds, sterols, intermediates of microbial sterol side-chain degradation or bile acids, together with 24 different steroids in a standardized test system. the resulting 11 beta-hydroxylation rate, leading to prednisolone 17-acetate and pred ... | 1992 | 1419889 |
| paronychia and black discoloration of a thumb nail caused by curvularia lunata. | chronic paronychia associated with black discoloration of the left thumb nail in a 51 year old female caused by curvularia lunata is reported for the first time. the keratolytic activity of the fungus in the nail and its complete clearance by topical clotrimazole are reported. | 1992 | 1435946 |
| cloning and molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase-encoding gene and cdna from the plant pathogenic fungus glomerella cingulata. | the glyceraldehyde-3-phosphate dehydrogenase gene (gpda) has been identified from a genomic dna library prepared from the plant pathogenic fungus glomerella cingulata. nucleotide sequence data revealed that this gene codes for a putative 338-amino-acid protein encoded by two exons of 129 and 885 bp, separated by an intron 216 bp long. the 5' leader sequence is also spliced by an intron of 156 bp. a cdna clone was prepared using the polymerase chain reaction, the sequence of which was used to con ... | 1992 | 1452034 |
| immobilized beta-glucosidase from curvularia lunata. | beta-glucosidase from curvularia lunata was immobilized in pellets of polyacrylamide, sodium alginate and agar. the activity of the enzyme was estimated at different times by measuring the absorbance of a solution into which 2-nitrophenol was released by the enzyme. the effect of ph and temperature was studied to select the optimum conditions. thermostability of the beta-glucosidase in each of the carriers was assessed over a period of 12-26 d. the immobilized enzyme on all the three carriers re ... | 1992 | 1452095 |
| some nutritional requirements of a mixed culture transforming reichstein's compound s into prednisolone. | reichstein's compound s was successfully converted to prednisolone in a single-step fermentation using a mixed culture of curvularia lunata and mycobacterium smegmatis. introducing additional medium at the time of bacterial inoculation and increasing the m. smegmatis inoculum to 8% were necessary for maximal dehydrogenation of cortisol to prednisolone (86%). however, beef extract, corn-steep solids, and malt extract were inhibitory to the dehydrogenase activity and stimulatory to hydroxylase. of ... | 1992 | 1458367 |
| sequence analysis of the gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of podospora anserina: use of homologous regulatory sequences to improve transformation efficiency. | the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of podospora anserina has been isolated from a genomic library by heterologous hybridization with the corresponding gene of curvularia lunata. the coding region consists of 1014 nucleotides and is interrupted by a single intron. the amino-acid sequence encoded by the gpd gene shows a high degree of sequence identity with the corresponding gene products of various fungi. multiple alignments of all fungal gpd sequences so far available result ... | 1992 | 1563046 |
| eumycetoma caused by curvularia lunata in a dog. | curvularia lunata was cultured from black granules found in granulomatous tumefactions excised from the subcutis of a three year old medium schnauzer dog. draining sinuses were present in some of the tumefactions. accordingly the diagnosis of eumycotic mycetoma was made. this diagnosis was confirmed by histopathological examination. during the four years following the first surgical intervention, several more similar tumefactions were excised on three different occasions. the dog died of chronic ... | 1991 | 1664052 |
| unusual aspects of allergic bronchopulmonary fungal disease: report of two cases due to curvularia organisms associated with allergic fungal sinusitis. | we report two cases of allergic bronchopulmonary fungal disease (abpfd) caused by curvularia sp and associated with allergic fungal sinusitis (afs). curvularia lunata was cultured in one case and curvularia senegalensis was cultured in the other. based on these cases and a review of the literature, we discuss unusual clinical and pathologic features that can occur in abpfd. unusual clinical aspects of abpfd include associated afs, absence of asthma, progression to churg-strauss angiitis and gran ... | 1991 | 1748430 |
| altered expression of the steroid bioconverting pathway in pan 7-1 transformants of cochliobolus lunatus. | the filamentous fungus c. lunatus converts progesterone mainly to its 11 beta-hydroxy derivative. c. lunatus transformed with the plasmid pan 7-1, which contains the e. coli hph gene expressed under the control of the a. nidulans gpd and trpc expression signals, lacks this activity, but exhibits acetyl side chain degradation of progesterone through the reaction scheme progesterone----20 beta-hydroxy-progesterone----delta 4-androstene-3,17-dione---- testolactone + testosterone. the main part of t ... | 1991 | 1807829 |
| genome analysis of imperfect fungi: electrophoretic karyotyping and characterization of the nuclear gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of curvularia lunata. | the gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) has been isolated from a genomic library of the filamentous fungus curvularia lunata. the coding region of this gene consists of 1014 nucleotides and is interrupted by four introns. the gpd gene product shows a high degree of sequence identity with the corresponding proteins of various species belonging to both taxonomically related (e.g., aspergillus nidulans), as well as more divergent, taxa. using contour-clamped homogeneous e ... | 1991 | 1934112 |
| induction of steroidal 11 beta-hydroxylase of cochliobolus lunatus. | the induction of steroidal 11 beta-hydroxylase of cochliobolus lunatus was studied and the effect of the structure of steroid inducers and additives on the extent of enzyme induction determined. 21-hydroxyprogesterone was found to be the best inducer and torlak peptone 1 the best additive. a certain parallelism was found between the induction of 11 beta-hydroxylase of cochliobolus lunatus and 11 alpha-hydroxylase of rhizopus nigricans. | 1991 | 1997119 |
| heterologous transformation of cochliobolus lunatus. | a dna mediated transformation system has been developed for the filamentous fungus cochliobolus lunatus. transformants were obtained by using plasmid pan 7-1 carrying the escherichia coli hygromycin b phosphotransferase gene (hph) fused to an aspergillus nidulans promoter. the integration of plasmid pan 7-1 into the fungal genome altered the ability of this microorganism to transform progesterone. | 1991 | 2037226 |
| macroscopic pigmentation in a dematiaceous fungal keratitis. | the diagnosis of fungal keratitis can be difficult and is often delayed. the distinction between moniliaceous and dematiaceous (pigmented) keratomycoses is not commonly possible on clinical examination. we report a case of a curvularia lunata fungal keratitis in a 40-year-old patient who presented with diffuse brown pigmentation throughout the ulcer bed. histologic staining and growth on sabourad's dextrose agar demonstrated the brown pigmentation characteristic of this pigmented fungus. we call ... | 1991 | 2055034 |
| allergic fungal sinusitis secondary to dermatiaceous fungi--curvularia lunata and alternaria. | allergic fungal sinusitis is a newly recognized entity consisting of a pansinusitis with allergic mucinous infiltrates in all involved sinuses. the disease process itself is very different from all other types of rhinosinusitis. pathophysiologically the disease is a combination of both ige-mediated and antibody-antigen reactions to the specific fungal antigens. here we present two cases of allergic fungal sinusitis--one caused by curvularia lunata species (an ubiquitous soil fungi), another caus ... | 1990 | 2117728 |
| 11 beta-hydroxylation of steroids by cochliobolus lunatus. | the substrate specificity of 11 beta-hydroxylase of cochliobolus lunatus was studied and a close parallelism to the results obtained with 11 alpha-hydroxylase of rhizopus nigricans was observed. it was found that the cell wall does not differentiate between the steroid substrates used and the absence of the cell wall increases the bioconversion. | 1990 | 2355739 |
| experimental pheohyphomycosis and mycotoxicosis by curvularia lunata in albino rats. | a rice contaminating fungus 'curvularia lunata' yields 'curvularin', a potent mycotoxin. experimental pheohyphomycosis and mycotoxicosis were observed in albino rats. spore suspension produced localised lesion; with simultaneous steroid therapy produced lesion in distant organs. mycotoxin, curvularin, produced hepatic necrosis. | 1989 | 2592030 |
| fungal peritonitis caused by curvularia lunata in a patient undergoing peritoneal dialysis. | the authors present a case of a patient undergoing continuous ambulatory peritoneal dialysis (capd) who developed peritonitis with curvularia lunata and gram negative enterobacteriaceae. a review of the literature indicates that human infections caused by curvularia are uncommon despite the ubiquity of the organism in the environment. in this case, the organism was present in the tenckhoff catheter, lacking attachment to it, but obstructing the flow. treatment of fungal peritonitis during capd i ... | 1989 | 2683770 |
| biological activity of sulphur ylides. i. antimicrobial properties of some new carbonyl stabilized sulphonium ylides. | antimicrobial properties of some new carbonyl stabilized sulphonium ylides have been tested against two bacteria staphylococcus aureus, escherichia coli and two fungi alternaria alternata and curvularia lunata. the potent antifungal activity of these compounds suggest their practical applications in fungicidal formulations and other pharmacological preparations. | 1989 | 2684160 |
| microbial transformations of steroids--iv. 6,7-dehydrogenation; a new class of fungal steroid transformation product. | microbial steroid dehydrogenation is quite common. the reaction seems to occur mainly in bacteria and usually results in hydrogen abstraction from positions c(1)-c(2) and/or c(4)-c(5) with occasional aromatisation of ring a. we have screened large numbers of fungal cultures for their ability to monohydroxylate steroids at unusual sites and in the course of our investigations we have identified seven fungal strains capable of dehydrogenating ring b of progesterone and androstenedione at positions ... | 1989 | 2770300 |
| [11 beta-hydroxylation of 16 alpha-methyl-reichstein's compound s 21-acetate by curvularia lunata]. | the conversion of 16 alpha-methyl-reichstein's compound s 21-acetate (i) to 16 alpha-methyl-hydrocortisone (ii) by the mycelium curvularia lunata as3.4381 was studied. maximal 11 beta-hydroxylating activity of the mycelium was found during cultivation of the microorganism by 24h. inhibition of the ethanol to the 11 beta-hydroxylating activity was obvious. the yield of 55.4% (ii) (w/w) could be achieved during conversion of 0.15% substrate at 72 h. | 1989 | 2800540 |
| hydroxysteroid dehydrogenase of cochliobolus lunatus. | cochliobolus lunatus is known to be able to hydroxylate steroids at position 11 beta. besides this inducible enzyme, we found a constitutive hydroxysteroid dehydrogenase activity which is strongly regioselective with the highest activity at position 17, and the best substrate was found to be androstenedione. using different substrates, no such activities were observed at positions 3 or 11. the enzyme is membrane bound and nadh or nadph dependent. the protoplasts of cochliobolus lunatus show the ... | 1988 | 2833663 |
| mycotic keratitis caused by curvularia lunata var. aeria. | a mycotic keratitis case which was caused by curvularia lunata var. aeria in a patient with an injury in the eye is described. the diagnosis was based on the mycologic analysis of several samples taken from the ulcer of cornea. in vitro tests of the sensitivity of the isolated species to several antifungal drugs were made. the results were related to the response in vivo to the treatment. | 1986 | 3485770 |
| fungal maxillary sinusitis caused by curvularia lunata. | recently, reports have surfaced in which saprobic fungi, as well as fungi pathogenic for plants, seem to be evolving as human pathogens. while the bulk of infected patients are immunocompromised, many individuals appear to be immunocompetent. to our knowledge, this is the second known published case of maxillary sinusitis caused by curvularia lunata in an immunocompetent patient. an interesting feature of this case is that the patient was treated with only surgery (débridement and irrigation) wi ... | 1987 | 3566951 |
| allergic fungal sinusitis due to curvularia lunata. | the clinical and pathologic features of allergic fungal sinusitis caused by curvularia lunata, as seen in two patients, are described. the findings are identical to those of allergic aspergillus sinusitis. patients have allergies, nasal polyposis, and, occasionally, eosinophilia. radiographs show opacification of multiple sinuses without bone destruction. surgical specimens consist of polyps and inspissated, mucoid material. diagnostic microscopic features, termed "allergic mucin," include eosin ... | 1987 | 3623555 |
| keratinophilic fungi from nigerian soil. | during a survey of keratinophilic fungi in nigerian soil samples, twelve species of fungi were isolated. these included chrysosporium keratinophilum, c. tropicum, curvularia lunata, microsporum audouinii, m. canis, m. fulvum, m. gypseum, m. vanbreuseghemii, trichophyton ajelloi, t. mentagrophytes, t. soudanense and t. yaoundei. these keratinophilic fungi had a higher incidence by occurrence of individual species during the rainy season (may-october) than the dry season (november-april). | 1987 | 3657901 |
| disseminated curvularia infection. additional therapeutic and clinical considerations with evidence of medical cure. | a previously reported case of cerebral infection due to curvularia lunata is more fully described. medical cure was apparently achieved after 30 months' treatment with amphotericin b. success was achieved only when the drug was given in a dose of 40 mg, three times per week, and was continued for six months after enhanced computed tomographic scans no longer showed cerebral lesions. immunologic studies suggested the infection was accompanied by an unexplained defect in cell-mediated immunity. | 1986 | 3767613 |
| human curvularia infections. report of five cases and review of the literature. | curvularia lunata is a saprobic dematiaceous mould that resides primarily in soil (ellis, 1966). reports of human disease caused by this organism are rare but include: endocarditis, brain abscess, skin infections, onychomycosis, keratitis, pneumonia, disseminated disease, mycetoma, allergic bronchopulmonary disease, and one case of sinusitis. since 1983, we have encountered five cases of paranasal sinusitis due to c. lunata. none of the patients suffered from known immunologic disorders or under ... | 1987 | 3802744 |
| disseminated curvularia infection. | curvularia is a saprophyte that rarely causes infection, and then usually occurs in immunocompromised patients. a 41-year-old man, previously in good health, developed pulmonary and cerebral infection with curvularia lunata. with surgery and amphotericin b therapy, he recovered and was free of infection one year later. | 1985 | 3839661 |
| enzyme-controlled reactions giving alkanols of use in the synthesis of biologically active molecules. | (+/-)-bicyclo[3.2.0]hept-2-en-6-one was reduced using a variety of fungi including yeasts. baker's yeast gave 6-exo-(1r,5s,6s)-bicyclo[3.2.0]hept-2-en-6-ol and 6-endo-(1s,5r,6s)-bicyclo[3.2.0]hept-2-en-6-ol while curvularia lunata and mortierella ramanniana gave only the 6-endo-alcohol and optically active bicycloheptenone. the optically active bicycloheptenols were used to prepare prostaglandin f2 alpha in the natural configuration. | 1985 | 3893940 |
| tenckhoff catheter obstruction resulting from invasion by curvularia lunata in the absence of peritonitis. | a 60-year-old black woman on continuous ambulatory peritoneal dialysis (capd) presented with tenckhoff catheter malfunction. she exhibited no signs or symptoms of acute peritonitis. darkly pigmented plaques were adherent to the inner wall of the catheter and exchange tubing focally throughout its length. similar material was adherent to the protein coagulum in the dialysis bag. the tenckhoff catheter and tubing were removed, and hemodialysis was initiated. cultures of the catheter and the dialys ... | 1985 | 4025332 |
| allergic bronchopulmonary curvulariosis. | a patient with clinical and roentgenographic findings suggestive of allergic bronchopulmonary disease who presented with recurrent pulmonary infiltrates with peripheral eosinophilia was identified. sputum cultures were positive for curvularia lunata. total serum ige was elevated, and intracutaneous skin testing with c. lunata was positive. serum precipitins against c. lunata were present with specific ige and igg antibody indexes elevated. these studies provide further immunologic characterizati ... | 1985 | 4040344 |
| microbial hydroxylations. ix. on the dihydroxylation of 19-norsteroids by curvularia lunata. | | 1974 | 4477359 |
| mycetomas caused by curvularia lunata, madurella grisea, aspergillus nidulans, and nocardia brasiliensis in sudan. | | 1973 | 4584205 |
| differences in mode of action of cellulolytic enzymes from curvularia lunata & chaetomium globosum. | | 1973 | 4779294 |
| hydroxylation of reichstein's compound s with cell-free preparations from curvularia lunata. | | 1968 | 4967077 |
| effect of certain vitamins on the growth and sporulation of curvularia lunata (wakker) boedijn. | | 1971 | 5166970 |
| microbial hydroxylations. iv. differential metabolism of 19-nor steroid antipodes by curvularia lunata. | | 1969 | 5388544 |
| microbial hydroxylations. vii. kinetic studies on the hydroxylation of 19-norsteroids by curvularia lunata. | | 1970 | 5499206 |
| microbial hydroxylations. 8. induction of steroid hydroxylases of curvularia lunata by 19-nortestosterone analogs. | | 1970 | 5499207 |
| microbial hydroxylations. vi. hydroxylation of rac-17 beta-hydroxyestra-4,8(14)-dien-3-one by curvularia lunata. | | 1970 | 5529207 |
| keratomycosis due to curvularia lunata. | | 1970 | 5529515 |
| [preparation of 6-alpha-fluoro-11-beta, 21-dihydroxy-16-alpha-methyl-1,4-pregnadiene-3, 20-dione (fluocortolone) via substrate-structure-directed specific 11-beta-hydroxylation by curvularia lunata]. | | 1969 | 5816548 |
| a case of onychomycosis caused by curvularia lunata (wakker) boedijn. | | 1983 | 6225016 |
| biological properties of acmycin, a new polyene antibiotic. | acmycin, a new polyene antibiotic, was fungistatic at low and fungicidal at high concentrations. it produced malformations in conidia of helminthosporium oryzae and curvularia lunata and caused leakage of yeast cells. acmycin was fairly nontoxic to germinating seeds. the intravenous ld50 was 300-400 and intraperitoneal maximum tolerated dose 100-500 mg/kg body mass of mice. | 1983 | 6618350 |
| composition of the fungal flora of four cereal grains in saudi arabia. | using the grain-plate method and on glucose- czapek 's agar at 28 degrees c, fifty-eight species belonging to 26 genera were collected from barley (42 species and 19 genera), maize (29 species and 16 genera), sorghum (32 species and 17 genera) and wheat grains (42 species and 18 genera). the most frequent genera were aspergillus, penicillium, rhizopus, fusarium, and mucor followed by alternaria, drechslera , and curvularia. from the preceding genera aspergillus flavus, a. niger, penicillium nota ... | 1984 | 6727980 |
| clinical and experimental keratitis due to curvularia lunata (wakker) boedijn var. aeria (batista, lima and vasconcelos) ellis. | a case of mycotic keratitis due to curvularia lunata var. aeria is reported for the first time from india. direct microscopic examination of the corneal scrapings in koh (50 g1(-1)), stained with lactophenol cotton blue, revealed brown septate hyphae and the cultures were found positive for fungus. it was identified as c. lunata (wakker) boedijn var. aeria (batista, lima and vasconcelos) ellis (imi 253204) and its growth was better at 28 degrees c than at 37 degrees c. in vitro, the fungus was m ... | 1982 | 6890237 |
| biochemical changes accompanying brefeldin a formation in curvularia lunata. | extracellular brefeldin a was detected in 4% glucose--peptone--mineral salts cultures of curvularia lunata at the start of the exponential growth phase. some fluctuations in brefeldin a levels occurred during the exponential growth phase followed by a significant reduction in level at the stationary growth phase. broth glucose levels decreased according to a sigmoid relationship with time whereas broth ph remained fairly constant during the exponential growth phase followed by a gradual increase ... | 1980 | 7191817 |
| synthesis of a series of new n1-[4-(4-nitrophenylthio)phenyl]-n3-(h/alkyl/acyl/aryl) thioureas and their antifungal, insecticidal and larvicidal activities. | a series of new mono and disubstituted thioureas, derived from 4-(4-nitrophenylthio) aniline, have been prepared and screened for antifungal activity against alternaria alternata and curvularia lunata. their insecticidal and larvicidal activities have also been evaluated against adult male and female cockroaches (periplanata americana) and mosquito larvae (culex pipiens fatigans wied) of iiird and early ivth instar stage, respectively. the tabulated results reveal that most of the thiourea deriv ... | 1981 | 7196731 |
| [degradation of steroids by microorganisms. xvii. 11 beta- and 12 beta-hydroxylation of (20s)-20-carboxy-1,4-pregnadien-3-one by cochliobolus lunatus]. | | 1981 | 7197433 |
| effect of substrates on the malonate inhibitory pattern and brefeldin a formation in curvularia lunata. | the extracellular level of brefeldin a fluctuates with the length of malonate inhibition. following treatment with malonate, mycelial multiplication as opposed to brefeldin a formation, was preferentially increased in the maleate, fumarate, succinate, citrate, methyl palmitate and glucose replacement cultures. competitive maleate--malonate, fumarate--malonate, succinate--malonate and citrate--malonate-inhibited replacement cultures gave significantly higher mycelial and brefeldin a yields than t ... | 1981 | 7203283 |
| curvularia lunata in experimental phaeohyphomycosis. | curvularia lunata strain sp, isolated from a disseminated human infection, infected normal mice, but three other strains of c. lunata and one each of c. pallescens and c. spicifera did not. the sp strain was recovered in cultures from, and hyphal filaments were observed in, abscesses in the liver and spleen of experimentally infected mice. all strains of curvularia infected mice treated with 400 rads x-irradiation and 10.0 mg cortisone, but at 400 rads and 5.0 mg only two strains of c. lunata (s ... | 1981 | 7278939 |
| allergic bronchopulmonary disease caused by curvularia lunata and drechslera hawaiiensis. | three patients who developed bronchoceles caused by fungi other than aspergillus sp are described. the first patient presented for investigation of a lesion at the right hilum on chest radiograph and a raised blood eosinophil count. a bronchogram showed complete block of the apical segmental bronchus which at operation was shown to be caused by inspissated material. the second patient was investigated because of a cough productive of plugs of sputum and irregular opacities in both upper zones on ... | 1981 | 7314001 |
| characterization of soluble rifamycin oxidase from curvularia lunata var. aeria. | curvularia lunata var. aeria was grown on yeast extract, peptone and carboxymethylcellulose (ypc) medium for the production of extracellular rifamycin oxidase. the enzyme was partially purified through a sephadex g-75 column. the half lives of rifamycin oxidase at 30 degrees and 40 degrees c were 9 d and 100 min, respectively. the activation and deactivation energies of the partially purified enzyme, calculated from arrhenius plots, were 5.80 and 35.10 kcal mol-1, respectively. the enzyme exhibi ... | 1993 | 7763786 |
| transformation of rifamycin b with growing and resting cells of curvularia lunata. | growing and resting cell systems of curvularia lunata were used for the transformation of rifamycin b to rifamycin s. in the case of growing cells, rifamycin b was added at the time of inoculation and at the different phases of growth. interestingly, it was found that c. lunata could grow in the presence of rifamycin b and could convert rifamycin b to rifamycin s. growing cells 24 and 48 h of age were capable of transforming rifamycin b. resting cells, cultivated at the exponential or early stat ... | 1993 | 7764294 |
| solvent selection and productivity in multiphase biotransformation systems. | the 11-beta hydroxylation of the steroid precursor reichstein's substance s (cortexolone) to hydrocortisone by curvularia lunata cells is used as a model system for investigating the basis of the effect of solvent choice on multiphase biotransformation reaction kinetics. the effect of solvent choice and phase ratio on cellular toxicity and productivity is examined in batch suspension cultures. in general, the greater the miscibility of the solvent with the aqueous phase, the more cellular toxici ... | 1994 | 7764676 |
| chemical-microbiological synthesis of ent-13-epi-manoyl oxides with biological activities. | the biotransformation of ent-13-epi-3-keto manoyl oxide, which possesses antileishmania activity, with curvularia lunata produced ent-6 beta-hydroxy, ent-1 alpha-hydroxy, ent-11 beta-hydroxy and delta 1-derivatives, as well as a reduction product a c-3 (s-alcohol) with another hydroxyl group at c-6 (ent-6 beta) or c-11 (ent-11 beta). the ent-6 beta-hydroxy and delta 1-derivatives inhibited growth of the pathogenic protozoa, leishmania donovani. the biotransformation of ent-12 alpha-acetoxy-3 bet ... | 1994 | 7765688 |
| fungal sternal wound infection due to curvularia lunata in a neonate with congenital heart disease: case report and review. | we describe a neonate with congenital heart disease in whom a sternal wound infection caused by the filamentous fungus curvularia lunata developed following cardiac surgery. despite their widespread distribution in the environment, curvularia species rarely cause human infection. we also review the 43 cases of curvularia infection previously reported in the english-language literature; only four of these cases occurred in children. a wide spectrum of infections--including keratitis, cutaneous in ... | 1994 | 7803640 |
| isolation and identification of testosterone and androstenedione in the fungus cochliobolus lunatus. | the cytosol of the filamentous fungus cochliobolus lunatus was found to contain binding proteins for testosterone and androst-4-ene-3,17-dione. both of these steroids were also found to be good exogenous substrates for the constitutive 17 beta-hydroxysteroid dehydrogenase, also found in this fungus. we were looking for the possible endogenous substrate. the procedures for isolation and identification of the endogenous steroid molecules in the fungus c. lunatus are described in this paper. the li ... | 1994 | 7940613 |
| curvularia lunata peritonitis complicating peritoneal dialysis. | a case of peritonitis due to curvularia lunata during continuous ambulatory peritoneal dialysis is reported. diagnosis was established by culture of dialysis effluent and peritoneal exudate, and was also confirmed through histological examination. | 1994 | 7984213 |
| progesterone metabolism by the filamentous fungus cochliobolus lunatus. | studies of cochliobolus lunatus m118 steroid metabolism by thin-layer chromatography, mass spectrometry and nmr spectroscopy revealed that the fungus hydroxylates progesterone at positions 7 alpha, 11 beta and 14 alpha, and oxidizes the 11 beta-hydroxy group to the ketone. the 1h nmr spectra of two of the steroid metabolites, 11 beta,14 alpha-dihydroxyprogesterone and 11-oxo-14 alpha-hydroxyprogesterone, are reported for the first time. it is still not known if all the hydroxylation reactions ar ... | 1994 | 8003444 |
| studies on rifamycin oxidase immobilized on kappa-carrageenan gel. | rifamycin oxidase from curvularia lunata var. aeria was immobilized on kappa-carrageenan gel where the enzyme showed excellent catalyzing activity and operational stability. factors affecting the activity of immobilized enzyme preparation such as ph and temperature were investigated. thermostability of the immobilized enzyme preparation was checked at 30 and 40 degrees c and it was found that the thermostability of the immobilized rifamycin oxidase activity has increased compared to free enzyme. ... | 1993 | 8117856 |
| characterization of rifamycin oxidase immobilized on alginate gel. | rifamycin oxidase of curvularia lunata was immobilized on alginate gel. the ph and temperature optima of the immobilized enzyme preparation were 6.5 and 50 degrees c, respectively. transformation reaction was carried out with the immobilized enzyme preparation. it took 8 h for the complete transformation of rifamycin b (10 g/l) to rifamycin s. the immobilized enzyme preparation was found to be mechanically weak even in the presence of cacl2 in the reaction mixture. reusability studies showed tha ... | 1993 | 8117857 |
| [gum-like exudate from laguncularia racemosa (white mangrove) as culture media for fungi]. | morphological studies of eight species of fungus: aspergillus flavus microsporum canis, epidermophyton floccosum, curvularia lunata, cladosporium carrionii, natrassia mangífera (edo. scytalidium), sporotrix schenckii y rhizophus oligosporus, which belong to families mucedinaceae, dematiaceae and mucoraceae have been carried out in support medium based in gum exudate from laguncularia racemosa (mangle blanco). this native polimer contains galactose, arabinose, rhamnose, uronic acid and proteins. ... | 1993 | 8123711 |
| purification and properties of cytochrome p-450 (p-450lun) catalyzing steroid 11 beta-hydroxylation in curvularia lunata. | addition of 11-deoxycortisol to the culture medium of curvularia lunata induced the increase of cytochrome p-450 content and steroid 11 beta-hydroxylase activity. the enzyme in cell-free extract produces cortisol from 11-deoxycortisol in the presence of nadph and o2. the enzyme was partially stabilized by glycerol, 11-deoxycortisol, gsh and pmsf. the hydroxylation activity was strongly inhibited by carbon monooxide and sulfhydryl reagents. cytochrome p-450 located on the microsomal fraction was ... | 1993 | 8268203 |
| invasive sinusitis with intracranial extension caused by curvularia lunata. | curvularia lunata has previously been linked to localized sinus infection in immunocompetent hosts. we treated a case of pansinusitis with extensive bone destruction and intracranial extension caused by c lunata. curvularia lunata was identified when typical fungal macroconidia appeared on culture. numerous surgical procedures, in addition to a 12-month course of antifungal therapy consisting of 4 g of intravenous amphotericin b and an 8-month course of 400 mg of oral ketoconazole daily, were re ... | 1993 | 8323423 |
| curvularia lunata endocarditis treated with terbinafine: case report. | a 44-year-old man developed endocarditis due to curvularia lunata on a carpentier-edwards porcine heterograft with clinical involvement of the ring of the aortic valve and the aortic root. because curative surgery was considered to be extremely high risk, he was treated with antifungal drugs for nearly 7 years. initial treatment with amphotericin b and ketoconazole was followed by long-term treatment with terbinafine, an experimental allyamine derivative. no adverse effects were attributed to te ... | 1993 | 8448316 |
| androgen binding proteins in cochliobolus lunatus. | testosterone and 4-androstene-3,17-dione (androstenedione) binding proteins were found in the cytosol of cochliobolus lunatus, a filamentous fungus. the maximal androgen binding occurred by 120 min at 0 degrees c. androgen binding proteins were degraded by protease treatment and at high temperature. the mean kd determined for testosterone binding protein (tbp) by scatchard analysis was 4.5 nm and the maximum capacity bmax was 120 fmol/mg protein; for androstenedione binding protein (abp) the kd ... | 1993 | 8472920 |
| identification of progesterone binding sites in the plasma membrane of the filamentous fungus cochliobolus lunatus. | plasma membrane associated binding sites for progesterone have been identified in the filamentous fungus cochliobolus lunatus (c. lunatus). the kd for progesterone determined by scatchard analysis was 13.9 +/- 5.7 nm and the bmax was 250-360 fmol/mg protein. a broad ligand specificity of these binding sites is suggested by the observation that all tested steroids, regardless of their capability to act as inducers of the 11 beta-steroid hydroxylase, competed at 250-fold excess with [3h]progestero ... | 1993 | 8499335 |
| case report: phaeohyphomycosis due to curvularia lunata involving skin and subcutaneous tissue after an explosion at a chemical plant. | cases of phaeohyphomycosis due to dematiaceous fungi have been reported in increasing numbers and diversity. the optimal roles of antifungal chemotherapy and surgical debridement in the management of these infections have not been determined. a case of acute cutaneous and subcutaneous phaeohyphomycosis due to curvularia lunata after an explosion at a chemical plant is reported, in which the organisms may have been inoculated into the tissues by the force of the blast. no organisms were found by ... | 1993 | 8506899 |
| steroid hormone signalling system and fungi. | three components of the steroid hormone signalling system, 17 beta-hydroxysteroid dehydrogenase, androgen binding proteins and steroid hormone signalling molecule testosterone were determined in the filamentous fungus cochliobolus lunatus for the first time in a fungus. their possible role in c. lunatus is discussed in comparison with their role in mammalian steroid hormone signalling system. the results are in accordance with the hypothesis, that the elements of primordial signal transduction s ... | 1995 | 8590379 |
| new polyoxygenated ent-manoyl oxides obtained by biotransformation with filamentous fungi. | incubation of methyl (13r)-ent-16-hydroxy-8 alpha,13-epoxylabd-14-en-18-oate [4] with curvularia lunata yielded ent-1 beta-hydroxy [6] and ent-6 beta-hydroxy [7] derivatives, and that of methyl (13s)-ent-16-dihydroxy-8 alpha,13-epoxylabd-14-en-18-oate [5] with the same organism gave ent-11 beta-hydroxy [8], ent-6 beta-hydroxy [9], and ent-6 beta,11 beta-dihydroxy [10] derivatives. the incubation of substrates 4 and 5 with fusarium moniliforme afforded ent-1 beta-hydroxy derivatives (6 and 14, re ... | 1995 | 8594146 |
| studies on shared antigenic/allergenic components among fungi. | fungal allergens have been found to be one of the most prevalent aeroallergens in india. knowledge of shared/unique components among different fungi is necessary for proper diagnosis and treatment of patients allergic to fungi. in the present study, crude extracts (ce) of 11 common fungi (alternaria alternata, aspergillus flavus, asp. fumigatus, asp. niger, asp. tamarii, asp. versicolor, cladosporium herbarum, curvularia lunata, mucor hiemalis, penicillium citrinum, and fusarium solani) were cha ... | 1995 | 8607563 |
| microbial hydroxylation of 13-ethyl-17 beta-hydroxy-18,19-dinor-17 alpha-pregn-4-en-20-yn-3-one. | the microbial transformation of the racemic mixture of 13-ethyl-17 beta-hydroxy-18,19-dinor-17 alpha-pregn-4-en-20-yn-3-one (1) was investigated. rhizopus nigricans (as 3.2050), r. arrhizus (as 3.4523), aspergillus niger (as 3.2744), a. ochraceus (as 3.1408), and curvularia lunata (nrrl 4381) transformed 1 into its 10 beta-hydroxy derivative (2) as a major metabolite. biotransformation of 1 by aspergillus ochraceus as 3.1408 afforded 7 beta-hydroxy derivative (3) as the only product. | 1996 | 8837292 |
| purification and characterization of 17beta-hydroxysteroid dehydrogenase from the filamentous fungus cochliobolus lunatus. | 17beta-hydroxysteroid dehydrogenase (17beta-hsd) from the filamentous fungus cochliobolus lunatus was purified in three steps, yielding a protein of an apparent molecular mass of 28 kda. according to the obtained experimental data, the native form of the enzyme could be a dimer (60 kda) and/or a tetramer (120 kda). the enzyme was found to catalyse preferentially the reduction of steroid substrates using nadph as an electron donor. both androgens and estrogens are substrates for 17beta-hsd. kinet ... | 1996 | 9010336 |
| cochliobolic acid, a novel metabolite produced by cochliobolus lunatus, inhibits binding of tgf-alpha to the egf receptor in a spa assay. | cochliobolic acid (1), a novel biologically active natural product, is produced by submerged fermentation of cochliobolus lunatus. compound 1 was determined to be a novel polyketide possessing a substituted tetrahydrofuran ring, a conjugated polyene chain and a 1,2-diketone moiety, by interpretation of nmr, ms, and uv/vis spectroscopic data. compound 1 inhibits the binding of tgf-alpha to the egf receptor of the human epidermal cell line a431 in a spa assay with an ic50 of 1.6 microm. | 1997 | 9014347 |
| carbonyl reducing 17 beta-hydroxysteroid dehydrogenase from the filamentous fungus cochliobolus lunatus. | | 1997 | 9059664 |
| in vitro antifungal activity of pneumocandin l-743,872 against a variety of clinically important molds. | the in vitro activity of the new antifungal drug pneumocandin l-743,872 against 55 isolates of clinically important molds was examined by an adapted macrobroth dilution method for yeasts. pneumocandin l-743,872 exhibited in vitro antifungal activity against alternaria sp., aspergillus flavus, aspergillus fumigatus, curvularia lunata, exophiala jeanselmei, fonsecaea pedrosoi, paecilomyces variotii, and scedosporium apiospermum. the drug appeared to lack significant in vitro inhibitory activity ag ... | 1997 | 9257774 |
| microbial transformation of sclareolide. | the microbial transformation of sclareolide (1) by curvularia lunata afforded five oxidized metabolites identified as 3-ketosclareolide (2), 1 beta-hydroxysclareolide (3), 3 beta-hydroxysclareolide (4), 1 alpha,3 beta-dihydroxysclareolide (5), and 1 beta, 3 beta-dihydroxysclareolide (6). fermentations of 1 with aspergillus niger also produced metabolites 2-6. metabolites 2-5 were obtained by fermention with gibberella fujikuroii, while fermentation with fusarium lini afforded metabolites 4 and 5 ... | 1997 | 9358647 |
| a succinylanthranilic acid ester and other bioactive constituents of jolyna laminarioides. | methyl 2-[propanamide-2'-methoxycarbonyl] benzoate, fucosterol, trans-phytol and p-formylphenol were isolated for the first time from a methanolic extract of jolyna laminarioides. methyl 2-[propanamide-2'-methoxycarbonyl]-benzoate exhibited chymotrypsin inhibitory activity and also found to be active against escherichia coli and shigella boydii. fucosterol exhibited antifungal activity against curvularia lunata, stachybotrys atra and microsporum canis. | 1997 | 9423291 |
| microbial models of soil metabolism: biotransformations of danofloxacin. | danofloxacin is a new synthetic fluoroquinolone antibacterial agent under development for exclusive use in veterinary medicine. such use could lead to deposition of low levels of danofloxacin residues in the environment in manure from treated livestock. this study was conducted to evaluate the potential for indigenous soil microorganisms to metabolize danofloxacin. cultures of 72 soil microorganisms representing a diverse panel of bacteria, fungi and yeast were incubated with danofloxacin mesyla ... | 1997 | 9451835 |
| 11beta-hydroxysteroid dehydrogenase activity in progesterone biotransformation by the filamentous fungus cochliobolus lunatus. | progesterone biotransformation was examined in relation to hydroxylating and dehydrogenating enzymes of cochliobolus lunatus. 11beta-hydroxysteroid dehydrogenase activity (11beta-hsd) was located in cytosolic fraction and was nadp-dependent, inducible by progesterone and apparently uni-directional. several inhibitors of 11beta-hydroxysteroid dehydrogenase were tested; furosemide, glycyrrhizic-acid and carbenoxolone did not influence the dehydrogenation of 11beta-hydroxy-4-pregnene-3,20-dione to ... | 1997 | 9459201 |
| allergic fungal sinusitis in the southeastern usa: involvement of a new agent epicoccum nigrum ehrenb. ex schlecht. 1824. | alternaria alternata, aspergillus spp., bipolaris spicifera, curvularia lunata, epicoccum nigrum and fusarium solani were isolated repeatedly from groups of patients among 96 diagnosed with allergic fungal sinusitis (afs). epicoccum nigrum was obtained consistently from four patients, one of whom yielded mycelial masses consistent in morphology with e. nigrum. fifteen of the predominant fungi recovered from air samples from selected patients' residences included the same species isolated from th ... | 1997 | 9467107 |
| fungal keratitis caused by curvularia lunata, with successful medical treatment. | | 1997 | 9474332 |
| microbial hydroxylation of acetylaminosteroids. | an investigation of the microbial biotransformation of a range of 3 beta-, 17 beta-, and 20-acetylamino c18 to c21 steroids by microorganisms known to hydroxylate conventional steroids has been undertaken, using aspergillus ochraceus, bacillus megaterium, curvularia lunata, and rhizoputus arrhizus. a. ochraceus and b. megaterium gave products of 11 alpha- and 15 beta-hydroxylation, respectively. in the case of c. lunata, the products were predominantly those of this organism's normal c-11 beta- ... | 1998 | 9727096 |
| preliminary antifungal and cytotoxicity studies of extracts of ritchiea capparoides var. longipedicellata. | crude extracts obtained from the leaves, stem bark and roots of ritchiea capparoides var. longipedicellata were screened for in vitro antifungal activity using the agar tube dilution method. the leaf hexane, leaf methanol, stem bark methanol and root methanol extracts were tested using ten clinical strains of fungi at a concentration of 200 and 400 microg/ml, respectively. at 400 microg/ml, all four extracts inhibited the growth of six of the ten test fungi used in the study. inhibition of the g ... | 1998 | 9849635 |
| fontana-masson--stained tissue from culture-proven mycoses. | the fontana-masson stain can be used to detect melanin of dematiaceous fungi such as curvularia lunata and bipolaris hawaiiensis in tissue. this stain is often used to identify fungi that may not have an evident brown color in hematoxylin-eosin-stained sections. however, it is not clear whether hyphae of nondematiaceous fungi can be stained with the fontana-masson procedure. | 1998 | 9870861 |
| the use of cryopreserved apical protoplasts from curvularia lunata for electrotransformation. | an electroporation method, utilizing cryopreserved protoplasts, has been developed for the steroid 11-hydroxylating fungus curvularia lunata strain im 2901. protoplasts released from the apical parts of 24- and 48-h-old mycelia were suspended in cryopreservation buffer and stored at -75 degrees c for several weeks. the thawed and freshly prepared (control) protoplasts were electroporated with pan 7-1 plasmid carrying the escherichia coli hygromycin b resistance gene (hph) under the control of as ... | 1998 | 9871997 |
| a novel 17beta-hydroxysteroid dehydrogenase in the fungus cochliobolus lunatus: new insights into the evolution of steroid-hormone signalling. | 17beta-hydroxysteroid dehydrogenase (17beta-hsd) from the filamentous fungus cochliobolus lunatus (17beta-hsdcl) catalyses the reduction of steroids and of several o- and p-quinones. after purification of the enzyme, its partial amino acid sequence was determined. a pcr fragment amplified with primers derived from peptide sequences was generated for screening the coch. lunatus cdna library. three independent full-length cdna clones were isolated and sequenced, revealing an 810-bp open reading fr ... | 1999 | 9895285 |
| dermatomycosis of the toe web caused by curvularia lunata. | | 1998 | 10030080 |