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the bactericidal effect of isoascorbic acid combined with mild heat.the thermal inactivation of salmonella thompson, escherichia coli, staphylococcus aureus, clostridium perfringens, candida zeylanoides, enterococcus faecium and e. faecalis was accelerated by the addition of sodium isoascorbate (1 mmol/l) to phosphate-buffer heating medium but not to complex food mixtures. the lethal effect of isoascorbate was nullified by heating under anaerobic conditions or by the addition of catalase. the scavengers of hydroxyl radicals, mannitol and formate were not protect ...19892613595
malassezia and candida colonisation on glans penis of circumcised men.the malassezia yeast are members of the normal human cutaneous flora in adults. they also are reported as part of the microflora of the male genital region in mostly uncircumcised males. it has been reported that malassezia sympodialis and malassezia globosa are the most frequent yeast belonging to the resident microflora of the penis as in other human skin areas. the aim was to evaluate the prevalence of malassezia and candida yeast colonisation on the glans penis of circumcised males. impressi ...200516115108
isolation of a lactic acid bacterium and yeast consortium from a fermented material of ulva spp. (chlorophyta).microbiota in a fermented culture of ulva spp. was examined with the objective to characterize the type of fermentation and to obtain starter microbes for performing seaweed fermentation.200415546421
identification of yeasts isolated from poultry meat.in an assessment of the potential role of yeasts in the spoilage of poultry meat, the population and species diversity of yeasts were determined on 50 commercial raw and processed chicken and turkey product samples. initial populations (log10 cfu/g) ranged from less than 0.1 to 2.9, and increased by the expiration date to 0.4 to 5.1, respectively. 145 of 152 isolates were identified as belonging to 12 species. yarrowia lipolytica and candida zeylanoides were predominant, accounting for 39% and 2 ...200111426853
molecular characterization of yarrowia lipolytica and candida zeylanoides isolated from poultry.yeast isolates from raw and processed poultry products were characterized using pcr amplification of the internally transcribed spacer (its) 5.8s ribosomal dna region (its-pcr), restriction analysis of amplified products, randomly amplified polymorphic dna (rapd) analysis, and pulsed-field gel electrophoresis (pfge). its-pcr resulted in single fragments of 350 and 650 bp, respectively, from eight strains of yarrowia lipolytica and seven strains of candida zeylanoides. digestion of amplicons with ...200011010879
species-specific identification of candida krusei by hybridization with the ckf1,2 dna probe.the species specificity of the candida krusei dna fingerprinting probe ckf1,2 has been investigated. a total of 149 pathogenic and nonpathogenic fungal and bacterial dnas were screened with ckf1,2. the probe was cold labeled with peroxidase, and its specificity was assessed by using southern blot, dot blot, and colony blot hybridization. its sensitivity was determined by dot blot hybridization. the ckf1,2 probe proved to be species specific. it hybridized with dna for the 112 c. krusei strains s ...19968784578
ability of rapid yeast plus system to identify 304 clinically significant yeasts within 5 hours.the rapid yeast plus system (innovative diagnostic systems, norcross, ga.) is a qualitative micromethod that uses conventional and chromogenic substrates for the identification of medically important yeasts. the ability of the rapid yeast plus system to accurately identify 304 clinical yeast isolates within 5 h was evaluated. the rapid yeast plus method correctly identified 286 (94.1%) of strains to the species level without the need for additional tests. a further 12 strains (3.9%) were classif ...19968727877
synthesis and anticandidal activity of some imidazopyridine derivatives.new hydrazide derivatives of imidazo[1,2-a]pyridine have been synthesized and evaluated for anticandidal activity. the reaction of imidazo[1,2-a]pyridine-2-carboxylic acid hydrazides with various benzaldehydes gave n-(benzylidene)imidazo[ 1,2-a]pyridine-2-carboxylic acid hydrazide derivatives. their anticandidal activities against candida albicans and candida glabrata (isolates obtained from osmangazi university, faculty of medicine, eskisehir, turkey), candida albicans (atcc 90028), candida uti ...200818608774
the expression of potential colonization factors of yeasts isolated from fish during different growth conditions.three strains, rhodotorula rubra, rhodotorula glutinis, and candida zeylanoides, isolated from fish, were tested for the expression of putative tissue colonization factors. all strains were able to bind collagen type i, fibronectin, and laminin to various degrees after growing on various solid and broth media, while the binding to collagen type iv was sparse under all conditions tested. for the three strains tested, a very low cell surface hydrophobicity was shown for growth on various solid and ...19938131110
the yeasts of british fresh sausage and minced beef.three hundred and eighty three yeasts isolated from samples of unsulphited or sulphited sausages and skinless sausages and minced beef were characterized in detail. debaryomyces hansenii was the most commonly isolated yeast from most samples followed by candida zeylanoides and pichia membranaefaciens. the presence of sulphite in sausages did not appear to affect the numbers and range of yeasts present but did affect their relative proportions. a survey of one factory showed that meat intended fo ...19846486769
differentiation of yeasts growing on dry-cured iberian ham by mitochondrial dna restriction analysis, rapd-pcr and their volatile compounds production.the efficiency of mitochondrial dna (mtdna) restriction analysis, rapd-pcr and volatile compounds analysis to differentiate yeast biotypes involved in flavour development of dry-cured iberian ham throughout the ripening process is evaluated. for this purpose, 86 yeasts isolated from iberian hams in the main ripening stages at different industries of the four protected designations of origin of this product, were used. the combination of mtdna restriction analysis and rapd-pcr using the primer (g ...200919527832
transient fungemia and candida arthritis due to candida zeylanoides.a 37 year old man developed transient fungemia caused by candida zeylanoides following a kidney and pancreas transplant. for the next seven months the patient had signs and symptoms of right knee arthritis. candida zeylanoides was finally identified as the aetiological agent. treatment with intravenous amphotericin b was successful. to our knowledge this is the first report of candida zeylanoides arthritis.19873326745
candida zeylanoides: another opportunistic yeast.a patient with a long history of scleroderma and gastrointestinal malabsorption requiring total parenteral nutrition was admitted with candida zeylanoides fungemia. the yeast responded to therapy, but on two subsequent admissions for episodes of fever the blood cultures yielded the same yeast. the identity of the candida species was established biochemically by both the api (analytab) and vitek system approaches. c. zeylanoides atcc 20356 and atcc 7351 served as controls for these analyses and f ...19911684799
dna typing methods for differentiation of yeasts related to dry-cured meat products.rflp analysis of the its and 18s rdna, rapd-pcr using mini- and microsatellite primers and rflp analysis of mitochondrial dna were examined to discriminate yeasts related to dry-cured meat products at species and strain level. seven species and 35 strains of yeasts usually found in dry-cured meat products were tested. rflp analysis of the its1-5.8s rdna-its2 and 18s rdna did not allow the separation at species level of all of the species tested. rapd with a m13 primer was found to be useful for ...200616257467
influence of antimicrobial agents on the spoilage of a meat-based entomophage diet.the microbial decomposition of a meat-based entomophage diet presented in parafilm packets was investigated. considerable bacteria but not fungi were associated with components used to prepare the diet (i.e., hens' eggs, liver, and ground beef). at the initial sampling time, there were no differences among diet treatments in the size of bacterial or fungal populations. bacterial populations in diets not containing antibacterial agents rapidly increased and reached an asymptote by 24 h (approxima ...200415154441
occurrence and characterization of yeasts isolated from artisanal fiore sardo cheese.the occurrence of yeast microflora in artisanal fiore sardo cheese during ripening was studied. mean yeast counts ranged from 2.64+/-1 log(10) cfu ml(-1) in milk to 0.65+/-1 log(10) cfu g(-1) in 9 months cheese, with the higher counts observed in 48-h-old cheese. strains belonging to the prevalent species debaryomyces hansenii, kluyveromyces lactis, geotrichum candidum, candida zeylanoides and candida lambica were selected for technological and genotypic characterization. all d. hansenii strains ...200415240074
dynamics and characterization of yeasts during natural fermentation of italian sausages.a multiphasic approach was used to investigate the yeast ecology in italian fermented sausages. culture-dependent and -independent methods were applied to identify the yeast species during the maturation process and to characterize the numerically dominant species. plating analysis and subsequent molecular identification of the isolates highlighted the dominance of debaryomyces hansenii, but at least other three yeast species -candida zeylanoides, pichia triangularis and metschnikowia pulcherrim ...200616879421
identification of yeasts associated with milk products using traditional and molecular techniques.an integrated approach including phenotypic (morphological, biochemical and physiological characterization) and genotypic (rapd-pcr, sequencing of d1/d2 domain of 26s rrna encoding gene) methods was used for the identification of yeasts isolated from different milk products. there were 513 isolates in all, 460 ascomycetous and 53 basidiomycetous yeasts. the yeast isolates were characterized on the basis of their biochemical and physiological properties, and the d1/d2 domain of 26s rdna was seque ...200616943023
presence and changes in populations of yeasts on raw and processed poultry products stored at refrigeration temperature.a study was undertaken to determine populations and profiles of yeast species on fresh and processed poultry products upon purchase from retail supermarkets and after storage at 5 degrees c until shelf life expiration, and to assess the potential role of these yeasts in product spoilage. fifty samples representing 15 commercial raw, marinated, smoked, or roasted chicken and turkey products were analyzed. yeast populations were determined by plating on dichloran rose bengal chloramphenicol (drbc) ...200011139011
characterisation of yeast flora isolated from an artisanal portuguese ewes' cheese.the evolution of the yeast flora was studied for an artisanal semi-hard ewes' cheese made from raw milk. mean log10 yeast counts per gram of cheese body ranged from 2.7 to 6.4, with the higher counts observed after a ripening period of 30 days. the yeast population decreased thereafter and, at the end of curing process, reached values similar to those of the beginning. a total of 344 yeasts strains were randomly isolated from the curd and cheese body during the 60 days long ripening period. este ...200011014522
ce separation of proteins and yeasts dynamically modified by peg pyrenebutanoate with fluorescence detection.the optimized protocols of the bioanalytes separation, proteins and yeasts, dynamically modified by the nonionogenic tenside peg pyrenebutanoate, were applied in cze and cief with the acidic gradient in ph range 2-5.5, both with fluorescence detection. peg pyrenebutanoate was used as a buffer additive for a dynamic modification of proteins and/or yeast samples. the narrow peaks of modified analytes were detected. the values of the pi's of the labeled proteins were calculated using new fluorescen ...200717557360
biodegradation of azo dyes by the yeast candida zeylanoides in batch aerated cultures.a number of simple azo dyes was degraded in liquid aerated batch cultures by a strain of the yeast candida zeylanoides. the standard decolorization medium contained glucose as a carbon and energy source, and its ph was either controlled to 5.0-5.2, or allowed to decrease to 3.2-2.8, in the course of microorganism growth. the extent of colour removal in the culture medium was assessed through the decrease in dye absorbance of the supernatants. the extent of colour removal ranged from 44 to 90%, a ...199910204232
biodiversity of yeasts from illinois maize.microflora in wound sites of preharvest maize (including bacteria, yeasts, and filamentous fungi) may play a role in attracting insects to maize plants and may also interact with growth and mycotoxin production by filamentous fungi. as little data are available about the yeasts occurring on maize from the u.s. corn belt, samples of milled maize from experimental plantings at the university of illinois river valley sand field were analyzed. yeast counts showed slight yearly fluctuation and varied ...19979115092
yeast population during ripening of dry-cured iberian ham.the relationship between the superficial yeast population and the ripening conditions of iberian dry cured hams has been studied for three different locations. tentative identifications were carried out for 836 isolates. candida zeylanoides was the dominating yeast in early stages, whereas more than 99% of isolates from the surface of matured hams were identified as debaryomyces hansenii. a great diversity of strains of c. zeylanoides and d. hansenii was found. the characteristic pattern of isol ...19968796428
intracellular ph homeostasis plays a role in the tolerance of debaryomyces hansenii and candida zeylanoides to acidified nitrite.the effects of acidified-nitrite stress on the growth initiation and intracellular ph (ph(i)) of individual cells of debaryomyces hansenii and candida zeylanoides were investigated. our results show that 200 microg/ml of nitrite caused pronounced growth inhibition and intracellular acidification of d. hansenii at an external ph (ph(ex)) value of 4.5 but did not at ph(ex) 5.5. these results indicate that nitrous acid as such plays an important role in the antifungal effect of acidified nitrite. f ...200818539814
case report: candida zeylanoides infective endocarditis complicating infection with the human immunodeficiency virus.despite the frequent occurrence of mucosal candidiasis in patients infected with hiv, systemic candidiasis is uncommon and usually associated with intravenous catheters, parenteral nutrition, or antibiotics and neutropenia. most of the fungal isolates are usually candida albicans, candida tropicalis or candida parapsilosis. the authors report a case of infective endocarditis due to candida zeylanoides that occurred in a patient infected with hiv in the absence of the usual risk factors for syste ...19968783683
application of sscp-pcr fingerprinting to profile the yeast community in raw milk salers cheeses.bacteria and yeasts are important sensory factors of raw-milk cheeses as they contribute to the sensory richness and diversity of these products. the diversity and succession of yeast populations in three traditional registered designation of origin (r.d.o.) salers cheeses have been determined by using phenotypic diagnoses and single-strand conformation polymorphism (sscp) analysis. isolates were identified by phenotypic tests and the sequencing of the d1-d2 domains of the 26s rrna gene. ninety- ...200616464699
the effect of substrate on the radiation resistance of yeasts isolated from sausage meat.the radiation resistance of a selection of yeasts isolated from sausages was assessed in phosphate-buffered saline and in sausage meat. the yeasts candida zeylanoides, debaryomyces hansenii and trichosporon cutaneum exhibited sigmoidal survival curves in both substrates whilst the more sensitive sporobolomyces roseus exhibited an exponential survival curve in buffer but a sigmoidal curve in meat. irradiating c. zeylanoides, d. hansenii and t. cutaneum in sausage meat changed the shape of their s ...19968588892
candida zeylanoides causing candidiasis as tinea cruris.candida zeylanoides caused candidiasis as tinea cruris in 4 cases. candida zeylanoides were characterized by good growth of the strain in malt medium, potato dextrose agar and sabourard's agar. their colony was milky white and greasy with smooth surface. the diameter of the colony reaches 12 mm within a week. as candida type pseudohyphae but not hyphae, they do not produce ascospore and bolospore. carbon sources fermentation showed positive results. nitrogen sources assimilation agar (kno3) nega ...19938243128
two cases of onychomycosis due to candida zeylanoides.as the range of yeasts and filamentous fungi reported to cause onychomycosis has increased, uncommon genera or species have appeared in the literature. candida zeylanoides is an example of a yeast rarely involved in human pathology. between 1989 and 1991, two cases of onychomycosis due to this yeast were encountered in wollongong, nsw. there were similarities in the cases, as both involved finger- or thumb-nails and both patients were female (one sixty years of age and the other, seventy-two). e ...19938240183
isolation of phosphate-solubilizing fungi from phosphate mines and their effect on wheat seedling growth.three phosphate-solubilizing fungi, identified as penicillium expansum, mucor ramosissimus, and candida krissii, were isolated from phosphate mines (hubei, people's republic of china) and characterized. all the isolates demonstrated diverse levels of phosphate-solubilizing capability in national botanical research institute's phosphate growth medium containing rock phosphate as sole phosphate source. acidification of culture medium seemed to be the main mechanism for rock phosphate solubilizatio ...200919277482
comparison of updated vitek yeast biochemical card and api 20c yeast identification systems.the updated vitek yeast biochemical card (ybc) was compared with the api 20c by using 409 germ tube-negative yeasts and geotrichum spp. that were either clinical or proficiency sample isolates. the api 20c was the reference standard. the 409 isolates represented nine genera and 21 species. morphology agars were inoculated and interpreted for each isolate. the api 20c identified 406 isolates (99.3%), while the vitek ybc identified 367 (89.7%). both systems identified the majority of yeasts after ...19948051243
yeast diversity and dynamics in the production processes of norwegian dry-cured meat products.this study investigate the diversity and dynamics of yeasts in the production processes of one unsmoked and two smoked dry-cured meat products of a norwegian dry-cured meat production facility. a longitudinal observational study was performed to collect 642 samples from the meat, production materials, room installations and indoor and outdoor air of the production facility. nutrient rich agar media were used to isolate the yeasts. morphologically different isolates were re-cultivated in their pu ...200919505738
[conditions and dynamics of citrate, isocitrate and polyol excretion in yeast growth on glucose].the dynamics of metabolite excretions by candida guilliermondii, candida zeylanoides and torulopsis candida was studied during their batch cultivation in media with glucose excess and low concentrations of n, p or s sources; the latter were the growth limiting factors and determined the transition to phase of retardation and the stationary phase of growth. growth limitation with the above components in the presence of a carbon source results in the following: (a) the growth becomes linear and th ...19817198180
yeast infection in a beached southern right whale (eubalaena australis) neonate.a female southern right whale (eubalaena australis) neonate was found stranded on the western cape coast of southern africa. skin samples were taken the same day from three different locations on the animal's body and stored at -20 c. isolation through repetitive culture of these skin sections yielded a single yeast species, candida zeylanoides. total genomic dna also was isolated directly from skin samples. polymerase chain reaction analysis of the internal transcribed spacer region of the fung ...200919617479
[comparative study of candida zeylanoides and candida vinaria].candida zeylanoides (castellani) langeron et guerra 1920 and candida vinaria ohara, nonomura et yunome ex. m. th. smith 1973 were comparatively studied. the species were found to differ in the assimilation of nine carbon sources, the vitamin requirements, the resistance to elevated nacl concentrations, the ability to utilize hydrocarbons. the ultrafine structure of the cell wall and the type of budding in c. vinaria are as in ascomycetous yeasts while the cell wall structure and the formation of ...19807393008
five-year evaluation of bloodstream yeast infections in a tertiary hospital: the predominance of non-c. albicans candida species.this is a retrospective observational study of clinical and epidemiologic data from bloodstream yeast infections over 5 years (2004-2008) in a tertiary-care hospital. during this period, there were 52 such infections, at a rate of 2.4 per 1,000 hospital admissions. non-c. albicans candida species and other genera were responsible for 82% of infections, with c. tropicalis and c. parapsilosis being the most common. in 2008 no c. albicans infections occurred. several uncommon fungal pathogens were ...201020163281
the dynamics of the yeast community of the tagus river estuary: testing the hypothesis of the multiple origins of estuarine yeasts.yeasts are common inhabitants of different types of aquatic habitats, including marine and estuarine waters and rivers. although numerous studies have surveyed yeast occurrence in these habitats, the identification of autochthonous populations has been problematic because several yeast species seem to be very versatile and therefore mere presence is not sufficient to establish an ecological association. in the present study we investigated the dynamics of the yeast community in the tagus river e ...201020422287
combined ph and high hydrostatic pressure effects on lactococcus starter cultures and candida spoilage yeasts in a fermented milk test system during cold storage.the combined effects of high pressure processing (hpp) and ph on the glycolytic and proteolytic activities of lactococcus lactis subsp. lactis, a commonly used cheese starter culture and the outgrowth of spoilage yeasts of candida species were investigated in a fermented milk test system. to prepare the test system, l. lactis subsp. lactis c10 was grown in uht skim milk to a final ph of 4.30 and then additional samples for treatment were prepared by dilution of fermented milk with uht skim milk ...201020832684
dynamics and characterization of yeasts during ripening of typical italian dry-cured ham.the evolution of the yeast population during manufacturing and ripening of dry-cured parma ham was investigated. contamination levels ranged from 10(5) to 10(7) cfu/g on muscle surface, 10(4) to 10(6) cfu/g on covering fat and exceeded 10(7) cfu/g on spreadable fat mince ("sugna"). two hundred and sixty one yeast isolates underwent identification test, showing that the predominant species of yeast population during the whole maturing process were debaryomyces hansenii, candida zeylanoides, debar ...200717418308
yeasts isolation from bovine mammary glands under different mastitis status in the mexican high plateu.the mastitis subclinical and clinical in cows caused by fungi has been increased specially by yeast of the genus candida.201121349344
in vitro antifungal activity of phenothiazines and their combination with amphotericin b against different candida species.candidiosis is a mycosis that is currently increasingly affecting the population in consequence of its frequency and the severity of its complications, especially among immunocompromised hosts. in this work, the in vitro anticandidal activities of two phenothiazines (ptzs), chlorpromazine (cpz) and trifluoperazine (tfp), and their combinations with amphotericin b (amb) were tested against 12 different candida strains representing 12 species (candida albicans, candida glabrata, candida guillermon ...201121605196
anti-candida zeylanoides activity of some iranian plants used in traditional medicine.to assess anti-candida zeylanoides activity of the essential oils (eos) of five iranian medicinal plants and to determine the different components of the eos.201223518076
identification of candida species isolated from bovine mastitic milk and their in vitro hemolytic activity in western turkey.in this study, identification of 207 candida isolates, previously isolated from mastitic bovine quarter milk samples at the level of genus, was made using api 20 c aux system. the most frequently isolated species were candida krusei (34.8%), followed by candida rugosa (16.4%), candida kefyr (12.6%), candida albicans (10.1%), and candida tropicalis (9.2%). less common isolates were candida zeylanoides (5.8%), candida parapsilosis (4.3%), candida guilliermondii (3.4%), candida famata (1.9%), and c ...201019921462
[identification and biodiversity of yeasts from qula in tibet and milk cake in yunnan of china].to analyze the biodiversity and distribution of the yeast species of qula in tibet and milk cake in yunnan, and to provide essential data for the utilization of yeasts in the traditional dairy products of china.201021090253
occurrence and growth of yeasts in processed meat products - implications for potential spoilage.spoilage of meat products is in general attributed to bacteria but new processing and storage techniques inhibiting growth of bacteria may provide opportunities for yeasts to dominate the microflora and cause spoilage of the product. with the aim of obtaining a deeper understanding of the potential role of yeast in spoilage of five different processed meat products (bacon, ham, salami and two different liver patés), yeasts were isolated, enumerated and identified during processing, in the final ...200822063618
study of the counts, species and characteristics of the yeast population during the manufacture of dry-cured "lacón". effect of salt level.the aim of this work was to study the yeast population during the manufacture of dry-cured "lacón" (a spanish traditional meat product) and the effect of the salting time. for this study, six batches of "lacón" were manufactured with three different salting times (ls (3 days of salting), ms (4 days of salting) and hs (5 days of salting)). yeast counts increased significantly (p < 0.001) during the whole process from 2.60 to 6.37 log cfu/g. an increased length of salting time did not affect yeast ...201223498173
characterisation of the yeast and mould biota in traditional white pickled cheeses by culture-dependent and independent molecular techniques.artisanal white pickled cheese of western serbia is a product of complex microbial community which detection by culture-dependent method only is hampered by its limitations. thus, in the present study, we used a culture-independent, semi-quantitative technique based on construction of an internal transcribed spacer (its)-clone library from metagenomic dna. this approach, based on direct dna extraction followed by amplification of fungal internal transcribed regions (its) cloned into plasmid and ...201627027646
purification and characterization of a novel cold-active lipase from the yeast candida zeylanoides.cold-active lipases have attracted attention in recent years due to their potential applications in reactions requiring lower temperatures. both bacterial and fungal lipases have been investigated, each having distinct advantages for particular applications. among yeasts, cold-active lipases from the genera candida, yarrowia, rhodotorula, and pichia have been reported. in this paper, biosynthesis and properties of a novel cold-active lipase from candida zeylanoides isolated from refrigerated pou ...201526820306
a novel series of thiazolyl-pyrazoline derivatives: synthesis and evaluation of antifungal activity, cytotoxicity and genotoxicity.in the current work, new thiazolyl-pyrazoline derivatives (1-22) were synthesized and evaluated for their antifungal effects against pathogenic yeasts and molds using a broth microdilution assay. ames assay was carried out to determine the genotoxicity of the most effective antifungal derivatives. the cytotoxicity of the compounds (1-22) was also investigated against a549 human lung adenocarcinoma and nih/3t3 mouse embryonic fibroblast cells. among these derivatives, 2-[5-(4-fluorophenyl)-3-(5-m ...201525576739
biodiversity of yeast mycobiota in "sucuk," a traditional turkish fermented dry sausage: phenotypic and genotypic identification, functional and technological properties.in this study, yeasts from turkish fermented sucuks were identified and their functional and technological properties were evaluated. two hundred fifty-five yeast isolates were obtained from 35 different sucuk samples from different regions of turkey. the yeast isolates were determined as genotypic using 2 different polymerase chain reaction (pcr) methods (rep-pcr and rapd-pcr). functional and technological properties of including proteolytic, lipolytic, and catalase activities, tolerance to nac ...201425273925
characterization of yeast population and molecular fingerprinting of candida zeylanoides isolated from goat's milk collected in sardinia.the occurrence of yeast microflora in raw goat's milk collected from 62 dairy farms located in different areas of sardinia was evaluated. candida zeylanoides was the most frequently occurring species followed by different basidiomycetous species. in the strains isolated some biochemical characteristics of technological interest were investigated and a predominance of lipolytic yeast species was found. we employed a simple method of dna extraction that in a minimal time and with low-cost provided ...201019879008
case report of invasive, disseminated candidiasis with peripheral nodular cavitary lesions in the lung.we report a case of invasive candidiasis presenting as multiple lung nodules and cavitary lesions with minimal pleural effusion. candida infections of the lung are rare but can occur after hematologic dissemination of the yeast from other body sites, such as the skin and the gastrointestinal and genitourinary tracts. here, we describe the case of a 56-year-old female with a history of end-stage renal disease (esrd) who presented with fever, productive cough, and pulmonary nodules and cavitary le ...201727909682
application of issr-pcr for rapid strain typing of debaryomyces hansenii isolated from dry-cured iberian ham.yeast populations of dry-cured iberian ham isolated from seven industries in the province of badajoz were characterized by issr-pcr using the (cag)4 primer and pcr-rflp of the its1-5.8s rrna-its2 fragment, and identified by dna sequencing. a total of 242 isolates were analyzed, indicating the primary species present was debaryomyces hansenii at 80.9% of the isolates followed by candida zeylanoides at 10.3% of the isolates. the remainders of isolates were identified as yamadazyma triangularis, sp ...201424929738
candida zeylanoides as a new yeast model for lipid metabolism studies: effect of nitrogen sources on fatty acid accumulation.lipid homeostasis is well-known in oleaginous yeasts, but there are few non-oleaginous yeast models apart from saccharomyces cerevisiae. we are proposing the non-oleaginous yeast candida zeylanoides qu 33 as model. the aim of this study was to investigate the influence of the carbon/nitrogen ratio and the type of nitrogen source upon oil accumulation by this yeast grown on shake flask cultures. the maximum biomass was obtained in yeast extract (2.39 ± 0.19 g/l), followed by peptone (2.24 ± 0.05 ...201424879093
genotypic variation and antifungal susceptibly of candida zeylanoides clinical isolates.to investigate the dna fingerprinting of candida zeylanoides (c. zeylanoides) strains and the correlation between genotyping and antifungal susceptibility of c. zeylanoides.201424746719
evaluation of lactic acid bacteria and yeast diversity in traditional white pickled and fresh soft cheeses from the mountain regions of serbia and lowland regions of croatia.the goal of this study was the characterisation of indigenous lactic acid bacteria (lab) and yeasts isolated from nine white pickled (bg) and nine fresh soft (zg) artisanal cheeses collected in serbia and croatia. while lab were present in all of the cheeses collected, yeasts were found in all bg cheeses but only in three zg cheese samples. high lab and yeast species diversity was determined (average h'(l)=0.4 and h'(y)=0.8, respectively). the predominant lab species in white pickled (bg) cheese ...201323973841
the influence of debaryomyces hansenii, candida deformans and candida zeylanoides on the aroma formation of dry-cured "lacón".the volatile profile of dry-cured "lacón" that has been inoculated with three different yeasts were determined and compared with a non-inoculated dry-cured "lacón". yeasts (debaryomyces hansenii, candida deformans and candida zeylanoides) that were used as starter cultures in the present study were selected among yeasts that were isolated from native dry-cured "lacón" at different stages of ripening process. these starters were spread on dry-cured "lacón" surface in order to test their capacity ...201323102730
synthesis and the selective antifungal activity of 5,6,7,8-tetrahydroimidazo[1,2-a]pyridine derivatives.even though there are new classes of compounds now frequently used in treatment of fungal infections, the density of deeply invasive candidiasis has increased at least 10-fold during the past decade. furthermore, many infections due to candida species are actually refractory to antifungal therapy. in this present study, it was aimed to synthesize, new hydrazide derivatives of tetrahydroimidazo[1,2-a]pyridine and evaluate their anticandidal activity and cytotoxicity in vitro. the reaction of tetr ...201020106559
studies on hydrazone derivatives as antifungal agents.the increasing clinical importance of drug-resistant fungal pathogens has urged additional need to fungal research and new antifungal compound development. for this purpose, some n-(1-benzyl-2-phenylethylidene)-n'-[4-(aryl)thiazol-2-yl]hydrazone (1a-e) and n-(1-phenylbutylidene)-n'-[4-(aryl)thiazol-2-yl]hydrazone (2a-e) derivatives were synthesised and evaluated for antifungal activity. their antifungal activities against standard and clinical strands of candida albicans, candida glabrata, candi ...200818665994
the predominance, biodiversity and biotechnological properties of kluyveromyces marxianus in the production of pecorino di farindola cheese.pecorino di farindola is a handicraft cheese made by farmers on small scale using raw ewes' milk and pig rennet. in this study, yeast consortia were evaluated during pecorino di farindola making and ripening. molecular identification of 156 isolates was achieved by a combination of pcr-rflp of the 5.8s its rrna region and sequencing of the d1/d2 domain of the 26s rrna gene. kluyveromyces marxianus was the predominant species, while other species (pichia kudriavzevii, candida parapsilosis, candid ...201425038503
dna typing methods for differentiation of debaryomyces hansenii strains and other yeasts related to surface ripened cheeses.the discriminative power of its-pcr, its-pcr rflp and mitochondrial (mt)-dna rflp were evaluated for differentiation of yeasts of importance for surface ripened cheeses. in total 60 isolates were included. of these, 40 strains of the following species, debaryomyces hansenii var. hansenii, d. hansenii var. fabryi, saccharomyces cerevisiae, candida zeylanoides, kluyveromyces lactis and yarrowia lipolytica, were obtained from culture collections and 20 isolates of d. hansenii representing six diffe ...200111589549
characterisation of yeasts isolated from 'nduja of spilinga.the 'nduja of spilinga protected geographical indication (pgi) is a spreadable italian salami, obtained by using fat (50%), lean of pork (25%), chili pepper (25%) and nacl, stuffed into natural pork casing. its predominant flora is represented by yeasts, reaching at the end of seasoning values of 6 log cfu/g. considering the need to enhance and protect traditional local products, it seemed interesting to carry out a characterisation of yeasts of the 'nduja of spilinga pgi. a total of 127 strains ...201427800341
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