| torulopsis glabrata pneumonia in a malnourished woman. | a middle-aged woman developed malabsorption and severe protein-calorie malnutrition after a near-total gastrectomy for a perforated gastric ulcer. a transbronchial lung biopsy showed pulmonary infection with torulopsis glabrata. improvement in the patient's nutritional status was followed by clearing of the pneumonia without the need for antifungal chemotherapy. | 1979 | 116368 |
| the yeast mitochondrial atpase complex. subunit composition and evidence for a latent protease contaminant. | 1. the subunit compositions of the f1 (oligomycin-insensitive) and f1--f0 (oligomycin-sensitive) mitochondrial atpase complexes from saccharomyces cerevisiae have been examined by the highly resolving technique of sodium dodecyl sulphate-polyacrylamide slab gel electrophoresis using a discontinuous buffer system. when isolated in the presence of protease inhibitors, f1 and f1--f0 contained five and twelve bands, respectively; this contrasts with the four- and ten-band patterns seen previously us ... | 1979 | 153154 |
| membrane-mediated killing of saccharomyces cerevisiae by glycoproteins from torulopsis glabrata. | cell-free supernatants from cultures of torulopsis glabrata contained glycoprotein toxins that killed sensitive and killer strains of saccharomyces cerevisiae with single-hit kinetics. growing s. cerevisiae treated with the toxins showed a leakage of cellular potassium, partial dissipation of the adenosine triphosphate pool, and a coordinate shutdown of macromolecular synthesis. these pool efflux-stimulating toxins have been partially purified and at least three toxic glycoproteins have been sep ... | 1975 | 240809 |
| torulopsis glabrata infections in singapore: a 4-year study. | during the period 1971-4, torulopsis glabrata formed 42% of yeasts isolated from 5677 clinical specimens from patients with cancers, diabetes mellitus, chronic renal failure, pregnacy or major surgical operations complicated by mycotic infections. the significance of t. glabrata in these patients is discussed. | 1977 | 265338 |
| torulopsis glabrata--urinary tract infections in diabetic patients in singapore. | urinary tract infections result mostly from ascending infection by micro-organisms introduced by way of the urethra. bacteria are the usual causative agents. occasionally, yeasts notably candida albicans, are involved. females are more prone to acute infections than males because of shorter urethra and the higher risks of contamination in the females. | 1977 | 266895 |
| suppressive effect of 3-methylcholanthrene on phagocytic activity of mouse peritoneal macrophages for torulopsis glabrata. | the effect of 3-methylcholanthrene (mca) on the phagocytic activity of mouse peritoneal macrophages for torulopsis glabrata was investigated. macrophages were maintained in glass scintillation vials or on cover slips in leighton tubes with the use of hanks' balanced salt solution plus 30% horse serum. graded amounts of mca were incorporated into the medium and the macrophages were parasitized with viable cells of t. glabrata. macrophages from c3h mice, a strain highly susceptible to mca carcinog ... | 1979 | 285299 |
| [mouth infections with torulopsis glabrata]. | | 1979 | 290111 |
| mode of action of yeast toxins: energy requirement for saccharomyces cerevisiae killer toxin. | the role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12k release from cells as a measure of toxin action. the saccharomyces cerevisiae killer toxin bound to sensitive cells in the presence of drugs that interfered with the generation or use of energy, but it was unable to efflux 12k from the cells under these conditions. in direct contrast, the torulopsis glabrata pool efflux-stimulating toxin induced efflux of the yeast 42k poo ... | 1977 | 320190 |
| occurrence of human-associated yeasts in bivalve shellfish from long island sound. | candida parapsilosis, c. tropicalis, and torulopsis glabrata were the human-associated yeasts most frequently isolated from quahogs, oysters, and mussels collected from four estuarine areas along the northern shore of long island sound. some inconsistency and seasonal variation in the occurrence of these and other yeast species were noted. in particular, c. albicans densities were greatest during colder months in the more heavily polluted waters. a total of 347 yeasts were isolated and cultured ... | 1977 | 322610 |
| antifungal activity of 5-fluorocytosine as measured by disk diffusion susceptibility testing. | the susceptibilities of 216 isolates of pathogenic and commensal yeasts to 5-fluorocytosine were tested by a disk diffusion technique with use of 1- and 10-microgram disks. minimal inhibitory concentrations (mics) were determined by an agar dilution procedure with yeast morphology agar, ph 5.0, supplemented with 0.25 mg of thiamine/dl. zones of inhibition produced by the two disks were correlated with paired mic values. an mic of 16 micrograms/ml, the upper limit of probable clinical susceptibil ... | 1977 | 325150 |
| mycotic growth and soft denture lining materials. | mycotic flora was studied from the dentures and denture bearing mucosae of 39 persons who wore soft-lined (molloplast b) mandibular dentures and heat-cured acrylic resin maxillary dentures. fungal growth was detected in 85% of the mandibular dentures and in 44% of the maxillary dentures (p less than 0.001). on the mandibular mucosa fungal growth was revealed in 74% and on the mucosa of the maxilla in 69%. in connection with inflamed mucosae fungal growth was always detected on the mandibular den ... | 1977 | 331850 |
| preprototype of an automated microbial detection and identification system: a developmental investigation. | the automicrobic system is an automated, computerized instrument that uses highly selective media and an optical system for detection, enumeration, and identification of bacteria and some yeasts in 13 h. a preprototype instrument (automicrobic system-1) and its urine culture kit (identi-pak), developed for the detection, enumeration, and identification of eight species or groups of bacteria and of candida species and torulopsis glabrata in urine specimens, was evaluated during its development. a ... | 1977 | 334797 |
| automated microbiological detection/identification system. | an automated, computerized system, the automicrobic system, has been developed for the detection, enumeration, and identification of bacteria and yeasts in clinical specimens. the biological basis for the system resides in lyophilized, highly selective and specific media enclosed in wells of a disposable plastic cuvette; introduction of a suitable specimen rehydrates and inoculates the media in the wells. an automated optical system monitors, and the computer interprets, changes in the media, wi ... | 1977 | 334798 |
| synergistic action of amphotericin b and antioxidants against certain opportunistic yeast pathogens. | results of earlier turbidimetric growth experiments showed that certain antioxidants prolonged the antifungal activity of amphotericin b (ab) against candida albicans, presumably by retarding autoxidative destruction of the drug. viability studies were designed to examine this in more detail. subinhibitory concentrations of either butylated hydroxyanisole, n-propyl gallate, or nordihydroguaiaretic acid in combination with fungistatic levels of ab exerted synergistic fungicidal activity against t ... | 1978 | 348098 |
| torulopsis glabrata endophthalmitis after keratoplasty with an organ-cultured cornea. | fungal endophthalmitis developed in an elderly woman following penetrating keratoplasty with an organ-cultured cornea. an unsuccessful search was made for the source of the infection. modification in our sterility checks prior to surgery were made in hopes of preventing future infections. | 1978 | 350200 |
| comparative susceptibility of four kinds of pathogenic fungi to amphotericin b and amphotericin b methyl ester. | the activity of amphotericin b methyl ester was compared with that of amphotericin b, using candida albicans (34 isolates), torulopsis glabrata (12 isolates), filobasidiella neoformans (stat. conid. cryptococcus neoformans) (14 isolates), and coccidioides immitis (37 isolates) and tube dilution in a totally synthetic, completely defined medium (saamf) with inocula of 10(4) colony-forming units per ml. minimal inhibitory concentrations were read after 24 h at 34 degrees c for c. albicans and t. g ... | 1978 | 354519 |
| [sensitivity in vitro of opportunist yeasts (candida albicans and torulopsis glabrata) to flucytosine and concordance curves (author's transl)]. | the authors report the results of a study with 100 strains of c. albicans and 50 strains of t. glabrata: the objectives of this experiment were to exhibit the primary and secondary resistance of the clinical isolates of fungi against flucytosine (5-fc) :another objective was to establish the concordance curves for 5-fc, by mean of mic measurements and inhibition diameters by disk plates diffusion, determined simultaneously for the same strains. these curves make it possible subsequently to deter ... | 1978 | 358866 |
| characterization of yeast agglutinins in lavage fluid from lungs of rabbits. | rabbits were challenged intratracheally with 10(8) candida albicans or torulopsis glabrata, and their lungs were lavaged 5, 60, and 120 min later. initial lavage samples showed significant agglutination of yeasts, followed by the development of larger aggregates in association with alveolar macrophages. to investigate this early agglutination reaction, lungs of normal rabbits were lavaged with heparinized saline, and after alveolar macrophages were discarded, the cell-free lavage fluid was centr ... | 1979 | 379248 |
| assay for yeast susceptibility to 5-fluorocytosine and amphotericin b in a frozen microtiter system. | a microtiter plate method for determining susceptibility of yeasts to 5-fluorocytosine and amphotericin b, which uses color indicators to detect end points, is presented. the microtiter plates can be made in advance and stored frozen for at least eight weeks. forty-two isolates of candida albicans, 12 of candida tropicalis, and nine of torulopsis glabrata were tested. results showed good correlation with turbidity tube dilution susceptibility testing methods. the microtiter method is stable, eas ... | 1979 | 382827 |
| antifungal activity of tioconazole (uk-20,349), a new imidazole derivative. | tioconazole (uk-20,349), a new antifungal imidazole derivative, was compared with miconazole for activity in vitro against candida spp., torulopsis glabrata, cryptococcus neoformans, aspergillus spp., and dermatophyte fungi (trichophyton spp. and microsporum spp.). tioconazole was more active than miconazole against all the fungal species examined except aspergillus, against which both agents showed similar activity. both tioconazole and miconazole inhibited the growth of all fungi examined at c ... | 1979 | 464592 |
| control of the yeast torulopsis glabrata in the stomachs of glucose fed lambs by oral dosing with nystatin and amphotericin "b". | in the absence of specific dietary lipids, large amounts of ethanol (ethyl alcohol) are produced from glucose in the stomachs of ruminant neonates by the resident yeast torulopsis glabrata. using new born lambs, oral dosing with the antifungal antibiotics nystatin and amphotericin "b" was shown in samples of stomach contents, to control the ethanol production entirely, and to suppress the yeast numbers partially but not permanently. of 11 lambs receiving heavy and continous doses orally of the p ... | 1977 | 558083 |
| torulopsis glabrata fungaemia. a report of two cases. | two cases of torulopsis glabrata fungaemia are presented. the literature on detection of micro-organisms in peripheral blood and on systemic t. glabrata infection is briefly reviewed. microscopical examination of a buffy coat preparation, a simple and rapid procedure for diagnosing this condition, is described. a scheme of criteria which may be helpful in the diagnosis of clinically significant fungaemia is offered. | 1977 | 559352 |
| [the antigenic relationship between torulopsis glabrata and syringospora claussenii]. | the agglutinating serum against torulopsis glabrata antigen agglutinated syringospora claussenii, and the serum against this fungus cross-agglutinated torulopsis glabrata. cross agglutinations and absorption experiments show that there is a common antigenic determinant between torulopsis glabrata and syringospora claussenii. | 1977 | 560619 |
| torulopsis glabrata and vitamin a deficiency in dogs. | | 1977 | 563689 |
| isolation of torulopsis glabrata from a urine specimen of a labrador bitch with urolithiasis. | | 1977 | 564733 |
| [miconazole in the treatment of systemic infection due to torulopsis glabrata]. | | 1978 | 565561 |
| disk agar diffusion susceptibility testing of yeasts. | a disk agar diffusion method was developed for testing the susceptibility of rapidly growing yeasts in vitro. a totally defined, completely synthetic agar culture medium (synthetic amino acid medium, fungal) and clinical isolates of candida spp. and torulopsis glabrata were used. turbidimetric adjustment of cell suspensions resulted in standard, reproducible inocula, which gave sharp, clear zones of inhibition when applied by an agar overlay method. optimal disk loads were determined for amphote ... | 1978 | 568910 |
| ulcerative vaginitis due to torulopsis glabrata: a case report. | a patient with ulcerative vaginitis is presented. the differential diagnosis included malignant ulcer, chancroid, and granuloma venereum. torulopsis glabrata vaginitis, which was subsequently proven, responded successfully to clotrimazole suppositories. predisposing and related factors and isolation and identification procedures are discussed. | 1978 | 569709 |
| [radioimmunological differentiation of yeasts (author's transl)]. | a microtiter solid phase radioimmunoassay is described for the differentiation or microorganisms by using six species of yeasts as a model. rabbits were immunized with homogenized cells of the yeasts. the rabbit sera obtained were absorbed to homologous cells and after elution, the antibodies so obtained were labeled with 125i. cross-reacting antibodies were eliminated by absorption to the corresponding yeast cells (table 1). with this radioimmunoassay it was possible to identify 51 out of 52 st ... | 1978 | 569952 |
| activation of the alternative complement pathway by torulopsis glabrata. | torulopsis glabrata is a yeast-like fungus with increasing importance as an opportunistic human pathogen. the role of serum complement in resistance to this infection was studied by chemotactic and opsonic assays. the results indicate that t. glabrata activates complement via the alternative pathway. this may be an important mechanism in host immunity to this fungus. | 1979 | 570297 |
| torulopsis glabrata fungemia: a case report with a review of literatures. | | 1979 | 571450 |
| esophagitis caused by torulopsis glabrata. case report. | | 1979 | 572135 |
| torulopsis glabrata fungemia--a clinical pathological study. | the clinical findings, pathologic features, and outcome were investigated in 46 patients in whom torulopsis glabrata was isolated in 131 specimens of blood. nineteen of the patients had only a single positive blood culture and no evidence of systemic yeast infection, while 27 patients had a clinically significant fungemia based upon the occurrence of 2 or more positive blood cultures, or the combination of a positive blood culture and isolation of the organism from a closed body cavity or demons ... | 1979 | 574609 |
| effect of bilirubin on the fungicidal capacity of human neutrophils. | the effect of unconjugated bilirubin on the fungicidal capacity of human neutrophils was examined. per cent torulopsis glabrata killed in the presence of 1 x 10(-5) and 5 x 10(-5) m bilirubin was 72.7 +/- 2.5 and 40.9 +/- 7.2 respectively, compared to 81.4 +/- 4.1 in control (p less than 0.01 and p less than 0.001), respectively). this effect was not due to a concomitant inhibition of phagocytosis. the results suggest that jaundiced neonates may be more susceptible to fungal infections. | 1979 | 575578 |
| [the epidemiology of blastomyces infections with special reference to postoperative intensive care wards (author's transl)]. | 230 strains of blastomyces were isolated during a nine month's period in the course of routine laboratory examinations of faecal and other specimens. candida albicans was the most frequently present (151), followed by candida tropicalis (39) and torulopsis glabrata (16). during an environmental study in a postoperative intensive care unit 426 swabs were taken. 88 strains of blastomyces were cultured from 75 swabs. the observations indicate that candida albicans as a free-living organism lacks fa ... | 1977 | 594031 |
| a comparison of the killer character in different yeasts and its classification. | the interactions between 20 killer yeasts of various genera and species were examined. ten distinct groups were recognised with respect to killer activity and 10 distinct groups with respect to resistance to killer action. using both killing and resistance phenotypes, 13 classes of killer yeast were found. with the exception of torulopsis glabrata ncyc 388, non-saccharomyces strains of yeast were not killed by a member of the genus saccharomyes. the killer character of the 3 killing groups of sa ... | 1978 | 655699 |
| comparison of the in vitro antifungal activities of miconazole and a new imidazole, r41,400. | r41,400 is a recently synthesized, water-soluble imidazole. its antifungal properties were compared in vitro with those of miconazole using an agar dilution procedure and 175 isolates of human fungal pathogens. the filamentous forms of histoplasma capsulatum and blastomyces dermatitidis were inhibited by less than or equal to 2 microgram of either drug/ml; coccidioides immitis was inhibited by less than or equal to 0.5 microgram of r41,400/ml. among the yeasts tested, cryptococcus neoformans and ... | 1978 | 681800 |
| combined activity of minocycline and amphotericin b in vitro against medically important yeasts. | the capacity of minocycline to enhance the activity of amphotericin b against candida albicans, torulopsis glabrata, cryptococcus neoformans, and non-albicans candida was examined in vitro utilizing a time-killing curve technique. synergism was apparent at 4 h with 5 of 5 strains of c. albicans, 8 of 8 strains of c. neoformans, and 1 of 12 strains of non-albicans candida. synergism was apparent at 24 h with the remaining 11 strains of non-albicans candida and all 5 strains of t. glabrata. c. neo ... | 1978 | 708025 |
| [blastomycoid flora of the urogenital tract in nonpregnant and pregnant patients]. | vaginal and vulva smears and urine obtained by catheters from 232 pregnant and 251 non-pregnant women were examined for the growth of yeasts. these fungi could be grown from the vulvo-vaginal smears from 26,7% of the pregnant women. yeasts were found in 6,4% of the urine samples. in the case of non-pregnant women, fungi were found in 19,9% of the vulvo-vaginal smears, whereas 3,2% of the urine samples contained yeasts. the growth of yeasts was encouraged by the addition of various urine dilution ... | 1975 | 766498 |
| killing of torulopsis glabrata by saccharomyces cerevisiae killer factor. | the saccharomyces cerevisiae killer factor glycoproteins killed the pathogen torulopsis glabrata by a mechanism involving membrane damage. some other yeast species were unaffected by these glycoprotein toxins. | 1976 | 773297 |
| inhibition of potentially pathogenic yeastlike fungi by clotrimazole in combination with 5-fluorocytosine or amphotericin b. | clotrimazole (ctm) has a doubtful future with respect to use in treatment of the systemic mycoses. to assess the potential of ctm in combined drug regimens, antifungal effects of ctm together with 5-fluorocytosine (5-fc) or amphotericin b (amb) were tested in a synthetic liquid medium against candida albicans, candida tropicalis, and torulopsis glabrata. viable counts were monitored over a 48-h incubation period. weak inhibitory concentrations of ctm were tested in combination with levels of 5-f ... | 1976 | 779631 |
| effect of culture media on the antifungal activity of miconazole and amphotericin b methyl ester. | the capacity of four culture media to obfuscate the antifungal activity of miconazole and amphotericin b methyl ester was evaluated qualitatively by examination of five isolates each of candida albicans, candida tropicalis, candida parapsilosis, torulopsis glabrata, and cryptococcus neoformans, and quantitatively by determination of the absolute minimal inhibitory concentrations for a strain of c. albicans. miconazole, like the predecessor imidazole (clotrimazole), was antagonized by two complex ... | 1976 | 789787 |
| torulopsis glabrata infection in the oral cavity. | the first case report of an oral infection due to torulopsis glabrata is presented. this microorganism is a yeast-like fungus grouped in a similar family to the candida species. it has been isolated with increasing frequency from the oral cavity but, surprisingly, has not been reported as a pathogen in this site. in other anatomical sites, including the urinary tract, lung and gastrointestinal tract, torulopsis glabrata has been reported as a pathogen, at times resulting in a fatal outcome. howe ... | 1976 | 814222 |
| serological diagnosis of infection of the urinary tract by yeasts. | precipitins to mannan and cytoplasmic antigens of candida albicans and torulopsis glabrata were determined in 25 patients with colonization of the bladder urine by yeasts and in 25 control patients with bacteriuria. precipitins were present in 64% of the patients with funguria and in 56% of the patients with bacteriuria. there was no correlation between the reactions obtained and the clinical significance of the yeast colonization. it is suggested that a single precipitin test is unhelpful in th ... | 1976 | 824329 |
| respiratory-deficient mutants of torulopsis glabrata, a yeast with circular mitochondrial deoxyribonucleic acid of 6 mu m. | purified mitochondria from the petite positive yeast torulopsis glabrata contain a circular deoxyribonucleic acid (dna) with a length of 6 mum and a buoyant density of 1.686 g/cm3. this dna is absent from ethidium bromide induced respiratory-deficient mutants. | 1976 | 944184 |
| pneumonia associated with torulopsis glabrata in an aborted bovine fetus. | the second case of bovine abortion caused by torulopsis glabrata observed in examining more than 5000 fetuses is reported. the yeast was cultured from fetal abomasal content and fetal lung; attempts to isolate viruses and bacteria were unsuccessful. the yeast was seen in association with diffuse fetal pneumonia characterized by presence of polymorphonuclear leukocytes and macrophages within bronchioles, alveolar lumina and alveolar septa. macrophages containing many yeast cells were observed wit ... | 1976 | 944464 |
| further studies on the intracellular behavior of torulopsis glabrata. | the growth of torulopsis glabrata was inhibited in glucose-peptone broth containing 10 to 20% normal human serum. addition of iron to the medium diminshed the fungistatic effect. the intracellular growth of t. glabrata was remarkably restricted within mouse macrophages maintained in vitro, but this growth restriction was not caused by the limitation of iron imposed by the serum in the medium. the intracellular growth of t. glabrata within a very small percentage of the macrophages was not obviou ... | 1976 | 987021 |
| ethanolic fermentation of glucose by torulopsis glabrata in the stomachs of neonates of the horse, dog, goat and soay sheep. | | 1976 | 1033019 |
| postoperative acalculous cholecystitis due to torulopsis glabrata. | acute acalculous cholecystitis due to torulopis glabrata, an opportunistic yeast, developed postoperatively in a 70-year-old man who had an extremely complicated course after resection of an abdominal aortic aneurysm. the infection first appeared as an acute surgical abdomen, three days after resumption of solid food intake subsequent to a prolonged ileus and after 31 days of parenteral hyperalimentation. the condition was successfully treated by cholecystostomy; at the time of writing, six mont ... | 1976 | 1033752 |
| torulopsis glabrata endocarditis complicating aortic homograft valve treated with 5-fluorocytosine: case report with discussion of antifungal chemotherapy. | a case of torulopsis glabrata endocarditis occurring in a patient 14 months after aortic homograft valve replacement is reported. the infection was not controlled by amphotericin b which led to progressive renal impairment. re-operation was delayed by the development of multiple infarctions due to coronary emboli. the infection was subsequently eradicated by oral treatment with the newer antifungal agent, 5-fluorocytosine, but death of the patient eventually occurred from an arrhythmia related t ... | 1975 | 1055952 |
| evaluation of a dehydrated test strip for the detection of yeasts. | use of a dehydrated test strip for the detection of yeasts is compared with traditional culture on sabouraud's agar containing 50 mug/ml chloramphenicol. while the selective medium of the strip is satisfactory for the isolation of species of candida, torulopsis glabrata grows only very slowly. the strip has the advantage of a long storage life without deterioration, but a high cost may preclude general usage. the numbers of yeasts collected by a bacteriological swab disadvantages of the selectiv ... | 1975 | 1100682 |
| sensitivity of some human pathogenic yeasts and systemic fungi to myxin. | myxin, a relatively new antibacterial and antifungal antibiotic, produced by a species of sorangium, was used to investigate its effectiveness against some yeasts and dimorphic fungi associated with human diseases. results indicated that the minimal fungicidal concentrations (mfc) of myxin for candida albicans, c. krusei, c. parapsilosis, c. tropicalis, and torulopsis glabrata were 0.39-6.25 mug/ml, and for c. guilliermondii and c. tropicalis 12.5-25 mug/ml. the mfc for blastomyces dermatitidis ... | 1975 | 1171116 |
| detection of fungi in blood cultures. | in a retrospective study covering the period january 1972 to june 1974, recovery rates of bacteria and of fungi were generally equivalent with tryptic soy broth, thiol, thioglycolate, and columbia broth media (all under vacuum with carbon dioxide and sodium polyanetholesulfonate). an additional biphasic medium consisting of brain heart infusion broth and a brain heart infusion agar slant, which was inoculated only where fungal sepsis was suspected clinically, yielded significantly higher recover ... | 1975 | 1176606 |
| therapeutic failure of miconazole in the treatment of torulopsis glabrata infection in the vagina. | | 1975 | 1237791 |
| in vitro activity of amphotericin b, hamycin and their novel water-soluble compounds against pathogenic yeasts. | twenty-eight pathogenic isolates, 4 each of candida albicans, c. lusitaniae, c. parapsilosis, cryptococcus neoformans, torulopsis glabrata and trichosporon beigelii were tested for their in vitro sensitivity to amphotericin b (amb), hamycin (ha) and their novel water-soluble compounds, namely jai-amb (oral and injectable; patents pending) and jai-ha (jaimycin inc., walnut creek, calif., usa), using a standard double-dilution broth (1 ml/tube) procedure. the 2 novel compounds, namely jai-amb and ... | 1992 | 1286573 |
| a non-azole inhibitor of lanosterol 14 alpha-methyl demethylase in candida albicans. | 6-hydroxy-n-methyl-n-(2-[4-phenylphenyl] ethyl)-1,2,3,4-tetrahydro-1- napthalene methanamine (a60586), a new non-azole inhibitor of ergosterol biosynthesis in candida albicans atcc62376 has been identified. in whole cells a60586 produced a dose related reduction of [14c]acetate incorporation into ergosterol and a concurrent increase in the radiolabelling of 4,4-dimethylated sterols. similar observations were made with [14c]mevalonic acid lactone labelled cell free extracts. the ic50s for inhibit ... | 1992 | 1289352 |
| vaginal yeast flora of pregnant women in the cusco region of peru. | a study of the vaginal yeast flora in pregnant women living in cusco and in its region (peru), located approximately 3000 m above sea level, is reported. we observed 300 pregnant, healthy and non-diabetic women who attended a gynaecological clinic in the lorena, regional or ipss (instituto peruano de seguridad social) hospitals in cusco. a comprehensive clinical history was obtained from each patient. it included age, work, parity, time of pregnancy, use of contraceptives or antibiotics, type of ... | 1992 | 1291874 |
| [the first isolation of torulopsis glabrata (anderson) lodder from dental granulomas]. | three torulopsis have been isolated from two hundred or so dental granulomas. torulopsis glabrata has been observed in cases of ophthalmopathy, infections of the oral cavity, lung infections, endocarditis and septicaemia. apical lesions due to torulopsis glabrata are not typical of mycosis but are generic of dental granulomas. the histological granulomatous character prevails. experimental infections have been obtained from this fungus. human infections by torulopsis glabrata have been described ... | 1992 | 1293491 |
| effects of itraconazole on phagocytosis and killing of candida glabrata by polymorphonuclear leucocytes from guinea pigs. | itraconazole, a systemically active antifungal, was tested for its effects on microscopically assessed phagocytosis and killing of candida glabrata 233 in vitro. yeast cells were exposed to itraconazole in culture and guinea-pig peritoneal polymorphonuclear leucocytes were exposed to the drug injected intraperitoneally in vivo. at a concentration of 10(-7) m and with exposure times of 1 h, itraconazole pre-treatment of the leucocytes had no effect on the ability of pmnl to ingest or kill c. glab ... | 1992 | 1328134 |
| in vitro susceptibility of 245 yeast isolates to amphotericin b, 5-fluorocytosine, ketoconazole, fluconazole and itraconazole. | the in vitro activity of amphotericin b, 5-fluorocytosine, ketoconazole, fluconazole and itraconazole was tested against 245 yeast strains isolated from clinical specimens (68 candida albicans, 74 candida tropicalis, 43 candida krusei, 28 candida glabrata, 19 candida parapsilosis, 8 candida lusitaniae and 5 candida guilliermondii). an agar dilution method was employed to carry out testing. minimal inhibitory concentrations to restrain 90% of isolate growth (mic90) ranged from 0.12 to 2 mg/l for ... | 1992 | 1337508 |
| disruption analysis of metallothionein-encoding genes in candida glabrata. | candida glabrata harbors multiple genes encoding metallothionein (mt). we have disrupted mt-iia, an amplified locus, and mt-iib, a single-copy gene, to determine the roles of various mt genes in cuso4 resistance in c. glabrata. the concentration of cuso4 required to inhibit the growth by 50% (ic50) of a c. glabrata strain harboring an amplified mt-iia locus and a single-copy mt-iib and mt-i genes was 7 mm in a synthetic complete medium. the ic50 decreased to approx. 1 mm when the amplified mt-ii ... | 1992 | 1339367 |
| a microbiological study of vaginal discharge in women attending a malaysian gynaecological clinic. | vaginal discharge is a common complaint of women attending gynaecological clinics. the purpose of this study was to compare the occurrence of commonly implicated microorganisms in vaginal discharge amongst women with or without the complaint, attending a gynaecological and family planning clinic. the association of gardnerella vaginalis with bacterial vaginosis was also studied. it was found that there were no significant differences between the cases and controls in the isolation rate of gardne ... | 1992 | 1387449 |
| impact of the changing epidemiology of fungal infections in the 1990s. | the increase in fungal infections over the past decade is striking. this is particularly true for hospitalized patients where the rate of candidal bloodstream infection has increased by as much as 487% over the decade of the 1980s. this increase in fungal infections is accompanied by a significant excess mortality and excess length of stay in hospital. the emergence of "new" fungal pathogens such as candida krusei, torulopsis glabrata, fusarium and trichosporon beigelii is now recognized as a si ... | 1992 | 1396747 |
| colony morphotype on sabouraud-triphenyltetrazolium agar: a simple and inexpensive method for candida subspecies discrimination. | a new method of candida subspecies discrimination on sabouraud-triphenyltetrazolium agar is reported. five hundred sixty-two strains of candida and torulopsis glabrata, previously identified by conventional mycological methods, were studied. each strain received a three-letter code and a number based on its colonial morphology. sixteen morphotypes were found for candida albicans, 6 were found for candida parapsilosis, 4 were found for both candida guilliermondii and candida krusei, and 12 were f ... | 1992 | 1400981 |
| evaluation of the pro-lab id ring system for the identification of medically important yeasts. | we evaluated 151 coded isolates of medically important yeast species belonging to the genera candida, cryptococcus, geotrichum, rhodoturula, saccharomyces and torulopsis using the newly developed rapid pro-lab identification ring, pl 960 system (plid-ring). all isolates were concurrently identified by the api 20c and conventional procedures comprising macro- and micromorphology, assimilation and fermentation of various carbon and nitrogen compounds. the plid-ring system identified isolates of ca ... | 1992 | 1406902 |
| efficacy of fluconazole in the treatment of systemic fungal infections. | the efficacy of fluconazole in the treatment of systemic fungal infections was evaluated in an open non-comparative trial. a total of 48 patients with proven or suspected fungal infections were enrolled in 40 of whom efficacy was evaluable. candida albicans accounted for 90% of the infections. candida parapsilosis, candida glabrata, histoplasma capsulatum and aspergillus fumigatus caused the infection in one case each. fluconazole was administered at a dosage of 200-400 mg daily for a mean durat ... | 1992 | 1425709 |
| conversion in the peptides coating cadmium:sulfide crystallites in candida glabrata. | cultures of candida glabrata treated with cdcl2 form intracellular cd(ii) complexes that evolve with the time of culturing. initially, glutathione (gamma ecg) appears to be the major buffering component. one type of cd(ii)-glutathione complex exists as a cadmium:sulfide (cds) crystallite coated with glutathione. a time dependent change in the coating of the cds particles occurs with a decrease in the (gamma ecg) content and a corresponding increase in the abundance of (gamma ec)ng peptides with ... | 1992 | 1431888 |
| dual systemic mycosis caused by bipolaris spicifera and torulopsis glabrata in a dog. | | 1992 | 1448909 |
| detection of serum candida antigens by enzyme-linked immunosorbent assay and a latex agglutination test with anti-candida albicans and anti-candida krusei antibodies. | serum samples from 197 patients with and without candidiasis were assayed for candida albicans mannan and candida krusei mannan by an enzyme-linked immunosorbent assay (elisa) and a latex agglutination test (la) and for d-arabinitol by the enzymatic fluorometric method. of the 43 patients positive for c. albicans mannan (> or = 0.2 ng/ml), 34 were infected with c. albicans and 9 were infected with candida tropicalis. c. krusei mannan (> or = 0.3 ng/ml) was detected in 10 patients infected with c ... | 1992 | 1452696 |
| collaborative comparison of broth macrodilution and microdilution antifungal susceptibility tests. | a collaborative comparison of macro- and microdilution antifungal susceptibility tests was performed in five laboratories. mics of amphotericin b, fluconazole, flucytosine, and ketoconazole were determined in all five centers against 95 coded isolates of candida spp., cryptococcus neoformans, and torulopsis glabrata. a standard protocol with the following national committee for clinical laboratory standards subcommittee on antifungal susceptibility testing recommendations was used: an inoculum s ... | 1992 | 1452697 |
| case report 762. torulopsis glabrata spondylodiscitis as a late complication of an infected abdominal aortic graft. | | 1992 | 1465652 |
| characterization of an azole-resistant candida glabrata isolate. | a candida (torulopsis) glabrata strain (b57149) became resistant to fluconazole after a patient carrying the organism was treated with the drug at 400 mg once daily for 9 days. growth of the pretreatment isolate (b57148) was inhibited by 50% with 0.67 microm ketoconazole, 1.0 microm itraconazole, and 43 microm fluconazole, whereas growth of b57149 was inhibited slightly by 10 microm ketoconazole but was unaffected by 10 microm itraconazole or 100 microm fluconazole. this indicates cross-resistan ... | 1992 | 1482129 |
| clamped homogeneous electric field gel electrophoresis typing of torulopsis glabrata isolates causing nosocomial infections. | thirty isolates of torulopsis glabrata were examined by pulsed-field gel electrophoresis, which resolved 13 dna pieces, allowing the identification of 12 types. bands at 1,400, 1,200, 1,070, 1,025, 681, and 500 kbp were conserved. when applied to 18 isolates from an outbreak, 10 distinct types were identified by this technique. seven patients had isolates which were identical. | 1992 | 1500538 |
| expression of a yeast metallothionein gene family is activated by a single metalloregulatory transcription factor. | the opportunistic pathogenic yeast candida glabrata elicits at least two major responses in the presence of high environmental metal levels: transcriptional induction of the metallothionein gene family by copper and the appearance of small (gamma-glu-cys)ngly peptides in the presence of cadmium. on the basis of a trans-activation selection scheme in the baker's yeast saccharomyces cerevisiae, we previously isolated a c. glabrata gene which encodes a copper-activated dna-binding protein designate ... | 1992 | 1508182 |
| purification and characterization of secretory proteinase of candida albicans. | proteolytic activity of medically important yeasts was tested in both ycb-bsa agar and medium. all of 134 strains of candida albicans, 13 of 18 strains of candida tropicalis and 11 of 18 strains of candida parapsilosis had this activity, while none of 52 candida glabrata strains or of 11 cryptococcus neoformans strains tested had proteolytic activity. strains of c. albicans fell into five groups based on the level and time-course of in vitro proteinase productivity. five strains randomly selecte ... | 1992 | 1522813 |
| fungal infections in cancer patients: an escalating problem. | major advances in anticancer treatment have contributed to an increased frequency of severe fungal infections in patients with neoplastic diseases. neutropenia remains the most important among the predisposing factors related to the malignancy or its treatment. most fungal infections are caused by the commonly recognized opportunistic fungi candida spp and aspergillus spp, and the pathogenic fungi cryptococcus neoformans, histoplasma capsulatum, coccidiodes immitis, and less often by blastomyces ... | 1992 | 1525339 |
| isolation and sequence analysis of the small subunit ribosomal rna gene from the euryhaline yeast debaryomyces hansenii. | the small subunit ribosomal rna gene (ssu rdna) from the euryhaline yeast debaryomyces hansenii has been isolated and sequenced. after appropriate alignment of this sequence with ssu rdna sequences from 30 other taxa, phylogenetic reconstruction using distance matrix and maximum parsimony methods indicates that d. hansenii is most closely affiliated with candida albicans, and occurs in the cluster of the yeasts saccharomyces cerevisiae, torulaspora delbruekii, candida glabrata, and kluyveromyces ... | 1992 | 1525872 |
| [renal and perirenal abscess and urinary tract obstruction caused by torulopsis glabrata infection]. | we report a case of renal and perinephretic abscesses due to torulopsis glabrata, in a diabetic women. fungal obstruction of upper urinary tract was associated. one must maintain a high suspicion for fungal infection when suppurative renal process occurs in a diabetic patient, especially when obstruction and absence of bacteria are noted. | 1992 | 1527400 |
| susceptibility of clinical isolates of fungi to saperconazole. | the in vitro activity of saperconazole against 228 strains of mycotic agents belonging to 48 species was investigated. susceptibility testing was performed using a microtiter broth dilution method. isolates of candida albicans, c. tropicalis and torulopsis glabrata showed distinct intra-species variation of susceptibility with mic values ranging from 0.045 to 100 mg l-1. the drug was inhibitory for the dermatophytes at a relatively narrow range of concentrations, most isolates being inhibited at ... | 1992 | 1528231 |
| saccharomyces cerevisiae and neurospora crassa contain heavy metal sequestering phytochelatin. | in fungi, cellular resistance to heavy metal cytotoxicity is mediated either by binding of metal ions to proteins of the metallothionein type or by chelation to phytochelatin-peptides of the general formula (gamma-glu-cys)n-gly. hitherto, only one fungus, candida glabrata has been shown to contain both metal inactivating systems. here we show by unambiguous fab-ms analysis that both a metallothionein-free mutant of saccharomyces cerevisiae as well as a wildtype strain synthesize phytochelatin (p ... | 1992 | 1534214 |
| [a case of intracranial germinoma responsive to amphotericin b]. | a 20-year-old man was admitted to our institution complaining of gradually worsening motor weakness in the right extremities. on admission, the radiological examination revealed a mass located in the left parietal region. on april 4, 1988, a left fronto-parietal craniotomy was performed and the tumor was subtotally removed. the pathological findings of the surgical specimen confirmed no specific tumor, but the presence of a small mingled candida glabrata supported the diagnosis of fungal granulo ... | 1992 | 1543364 |
| [shifts in the species spectrum of mycoses in the netherlands from 1970-1990]. | a review is presented of yeast and mould infections occurring in humans in the netherlands. the occurrence of the dermatophytes trichophyton rubrum and t. mentagrophytes tends to increase, while microsporum canis and particularly epidermophyton floccosum have become less common. the yeast candida glabrata is particularly often involved in infections of the urinary tract. candida krusei, c. parapsilosis and c. tropicalis have become less significant. remarkable differences are found between the s ... | 1992 | 1557162 |
| orofacial fungal infections. nine pathogens that may invade during chemotherapy. | immunosuppression due to antineoplastic drugs or malignant tumor leaves patients extremely vulnerable to infection. opportunistic fungi that rarely infect healthy persons can have very severe consequences in these patients. candida albicans is the pathogen found most often, but several other candida species may cause infection as well. aspergillosis is the second most frequently seen fungal infection of the face and mouth in patients receiving chemotherapy. less commonly seen but equally dangero ... | 1992 | 1561171 |
| candidemia in immunocompromised patients. | this study reviews data on patients with fungemia and confirms the high prevalence (50%) of infections caused by non-albicans species of candida. fungemia due to c. albicans or torulopsis glabrata occurred significantly more often in patients with a solid tumor, while fungemia due to candida tropicalis or candida krusei was significantly more common in patients with hematologic malignancy (p = .001). for 31% of patients, only a single blood culture was positive for yeasts, and the prognosis for ... | 1992 | 1562683 |
| cloning system for candida glabrata using elements from the metallothionein-iia-encoding gene that confer autonomous replication. | the yeast candida glabrata harbors two distinct gene families that encode metallothioneins (mts). one of these loci, the mt-iia locus, exhibits selective and tandem amplification in many wild type strains of c. glabrata. the present paper demonstrates that the amplified mt-iia gene contains autonomously replicating sequences (ars). these ars elements have been used to construct vectors capable of replicating in c. glabrata. the ars element(s) in the mt-iia gene were localized to a 457-bp segment ... | 1992 | 1563627 |
| structural study of a cell wall mannan-protein complex of the pathogenic yeast candida glabrata ifo 0622 strain. | we conducted a structural analysis of the cell wall mannan-protein complex (mannan) isolated from a pathogenic yeast, candida glabrata ifo 0622 strain. the chemical structure of mannobiose released from this mannan by treatment with 10 mm hcl at 100 degrees c for 1 h was identified as manp beta 1-2man. the treatment of this mannan with 100 mm naoh at 25 degrees c for 18 h gave a mixture of alpha-1,2- and alpha-1,3-linked oligosaccharides, from tetraose to biose, and mannose. the acid- and alkali ... | 1992 | 1567221 |
| vulvovaginal candidiasis refractory to treatment with fluconazole. | we present the case of an infertile patient, whose first attempt at ivf had to be postponed for 18 months due to a vulvovaginal yeast infection refractory to treatment. the main causative organism was a candida glabrata strain resistant to all the imidazolic agents tested. the organism and the host's humoral status were studied in depth, looking for possible causes of the refractoriness to treatment. | 1992 | 1587371 |
| torulopsis glabrata peritonitis in a chronic hemodialyzed patient. | | 1992 | 1630561 |
| surveillance of nosocomial fungal infections in a burn care unit. | a survey was conducted to trace the source of nosocomial fungal infections in the burn care unit of nehru hospital, chandigarh, india, by collection of samples from wounds of 25 severely burnt patients and their surroundings. the environmental sampling revealed predominant fungal contamination by dematiceous hyphomycetes, aspergilli, penicillium, fusarium and yeasts (candida albicans, candida tropicalis, candida krusei, candida parapsilosis), whereas the colonising or invading fungi from the pat ... | 1992 | 1644487 |
| [in vitro antifungal activity of itraconazole, a new triazole antifungal agent, against clinical isolates from patients with systemic mycoses]. | the in vitro antifungal activity of itraconazole (itz), a new oral triazole antifungal agent, against clinical isolates from patients with systemic mycoses were compared with those of existing systemic antifungals, viz. ketoconazole (kcz), miconazole or amphotericin b. the studies were performed with 65 isolates of pathogenic yeasts and 13 isolates of aspergillus spp. using the agar dilution method on casitone agar. itz showed the most potent antifungal activities against isolates of pathogenic ... | 1991 | 1652654 |
| in vitro comparison of cilofungin alone and in combination with other antifungal agents against clinical isolates of candida species. | cilofungin, a lipopeptide antifungal agent, was tested for in vitro activity alone and in combination with ketoconazole, itraconazole, flucytosine and amphotericin b against 102 clinical isolates of candida species. at 48 hours all isolates of candida albicans, candida tropicalis, candida paratropicalis and candida glabrata were inhibited by less than or equal to 5 meg/ml of cilofungin. in contrast, the mic90 for candida krusei was 10 mcg/ml and for candida parapsilosis greater than 40 mcg/ml. t ... | 1991 | 1655435 |
| safety and efficacy of itraconazole in prevention of fungal infections in neutropenic patients. | in a non-randomized study the efficacy of itraconazole in preventing fungal infections in neutropenic patients was investigated. forty-seven patients with acute leukemia or advanced lymphoblastic lymphoma were enrolled. ninety-two episodes of severe neutropenia after chemotherapy were observed. mean duration of neutropenia was 24 days. norfloxacin was administered as prophylaxis against gram-negative infections and itraconazole 200 mg b.i.d. as antifungal prophylaxis. surveillance cultures of th ... | 1991 | 1660108 |
| hospital outbreaks with yeasts. | five previous outbreaks of disseminated candidosis due to candida albicans are reviewed and a new outbreak on a neonatal unit in belfast presented. this involved four disseminated cases. the control and definition of outbreaks by morpho-, immunoblot- and dna-typing is discussed. an outbreak of torulopsis glabrata infection involving 23 patients is described. this was defined by dna fingerprinting with the enzyme xba. there were five deaths attributable either completely or in part to the yeast i ... | 1991 | 1679789 |
| a gene required for rnase p activity in candida (torulopsis) glabrata mitochondria codes for a 227-nucleotide rna with homology to bacterial rnase p rna. | we have mapped a gene in the mitochondrial dna of candida (torulopsis) glabrata and shown that it is required for 5' end maturation of mitochondrial trnas. it is located between the trnafmet and trnapro genes, the same trna genes that flank the mitochondrial rnase p rna gene in the yeast saccharomyces cerevisiae. the gene is extremely at rich and codes for au-rich rnas that display some sequence homology with the mitochondrial rnase p rna from s. cerevisiae, including two regions of striking seq ... | 1991 | 1705011 |
| reassessment of antigenic determinant of saccharomyces cerevisiae serotype ia. | we examined the immunochemical structure of the antigenic determinant of s. cerevisiae serotype ia. the specific factor serum for s. cerevisiae serotype ia was obtained either from factor 18 serum by adsorption with heat-killed cells of candida glabrata, or from anti-s. cerevisiae ia (m 6001) serum by adsorption with heat-killed cells of s. cerevisiae ib (ifo 0751). we designated this adsorbed serum as factor 18a. acetolysis of s. cerevisiae cell wall d-mannan gave five oligosaccharides. signals ... | 1990 | 1706464 |
| a cell surface/plasma membrane antigen of candida albicans. | antibody from balb/cbyj mice immunized against a membranous fraction of candida albicans agglutinated whole cells as well as the membranous fraction. hybridoma techniques were used to isolate an igm monoclonal antibody (mab) designated 10g which agglutinated whole cells and reacted with the subcellular fraction. yeast cells of 15 additional c. albicans strains and isolates of c. stellatoidea, c. tropicalis, c. intermedia and c. lusitaniae were also agglutinated by mab 10g. the antigen was not de ... | 1991 | 1709679 |
| hsp 90, yeasts and corynebacterium jeikeium. | recovery from disseminated candidosis is associated with seroconversion to a 47 kda breakdown product of the heat shock protein (hsp) 90 of candida albicans. cloning, sequencing and epitope mapping has allowed the delineation of the immunodominant epitopes lkvirknivkkmie and stdepagesa. monoclonal and polyclonal antibodies specific to these epitopes are used to show that all strains of c. albicans tested produce hsp 90 in both the yeast and mycelial phases. homologous proteins are demonstrated i ... | 1991 | 1718769 |
| electrophoretic karyotypes of clinically isolated yeasts of candida albicans and c. glabrata. | one-hundred-and-four isolates of yeast were collected from the vaginas of 97 outpatients. the isolates were identified by their characteristics in a carbohydrate assimilation test, a serological test and from their morphology. candida albicans and candida glabrata were the major isolates (75% and 20%, respectively). the karyotypes of the isolates were analysed by pulsed-field gel electrophoresis and almost all the karyotypes were distinguishable from one another when the band mobilities were car ... | 1991 | 1783899 |
| development of resistance in candida isolates from patients receiving prolonged antifungal therapy. | the impact of prolonged antifungal therapy on the development of resistance was examined in 61 patients with oropharyngeal thrush. fifty-nine patients had symptomatic human immunodeficiency virus infection, one had lung cancer, and one had metastatic prostate cancer. cultures of pharyngeal samples from all patients were positive for yeasts and included 57 (93.4%) candida albicans, 3 (4.9%) candida glabrata, and 1 (1.6%) candida krusii. of 61 patients, 32 (52.5%) were receiving or had recently re ... | 1991 | 1804002 |
| oral yeasts, mucosal health, and drug use in an elderly denture-wearing population. | drug use among the elderly may be a factor in oral mucosal pathology and the prevalence of oral yeasts. this study was designed to evaluate the relationship between oral yeast, denture-bearing mucosal health and drug use, especially drugs with known hyposalivatory side effects. there was an inverse relationship between denture-bearing mucosal health, and hyposalivatory drug use r = -0.41 p less than 0.04. use of drugs with a hyposalivatory side effect was related to increased numbers of candida ... | 1991 | 1813993 |