| gastric ph and microflora of normal and diarrhoeic infants. | the microflora and ph of gastric contents were determined in breast-fed and in bottle-fed normal infants, in well nourished infants with acute diarrhoea and in infants with chronic diarrhoea and protein-calorie malnutrition. the last group of infants was reevaluated after recovery from diarrhoea and protein-calorie malnutrition. a bactericidal ph effect below 2-5 was observed. bottle-fed controls had low ph values and low bacterial concentrations, whereas infants with chronic diarrhoea and prote ... | 1975 | 274 |
| comparative study of virological infections in asthmatic and nonasthmatic children. | the author shows complex analyses: clinical, laboratory, x-rays, bronchoscopical, bronchographical and measuring lung function tests as well as the serological examinations in blood serum of both groups of asthmatic and nonasthmatic children with virological infection. the calculation of statistically significant differences between the various diagnostical results of both groups has confirmed that in asthmatic children virological infection of the respiratory tract, pathological findings in x-r ... | 1975 | 884 |
| microbiol growth in lipid emulsions used in parenteral nutrition. | parenteral nutrition via central venous catheterization is associated with serious risks, especially that of sepsis. lipid emulsion (intralipidsweden), which may be administered peripherally, was evaluated for its potential to support microbial growth. washed cultures of staphylococcus aureus, candida albicans, and three species of gram-negative rods were all capable of multiplying in the emulsion at room temperature. variations in inoculum size did not affect the growth rate. studies comparing ... | 1975 | 982 |
| bacterial and fungal growth in total parenteral nutrition solutions,. | the most serious complication of prolonged intravenous infusion of hypertonic dextrose and amino acids is infection. frequently, the etiology is fungal rather than bacterial. previous authors have suggested that bacterial survival and growth in the solutions is suppressed by (a) high dextrose concentration, (b) high osmolality, or (c) low ph. this paper presents evidence that proposals (a) and (b) are untenable and (c) is only partly responsible. we call attention to the presence of a factor tha ... | 1975 | 2102 |
| significance of microorganisms found in throat swabs (author's transl). | | 1976 | 3776 |
| germination of candida albicans induced by proline. | blastospores of candida albicans germinated in proline-biotin-buffer medium incubated at 37 c. certain other amino acids in the glatamate, asparate, and pyruvate families also fostered germinaton but generally to a lesser extent than did proline. l-cysteine, d-proline, and certain structural analogues of l-proline inhibited proline-stimualted germination. the concentration of phosphate and glucose was crucial to amino acid-stimulated germination of c. albicans. clinical isolates and stock cultur ... | 1976 | 5375 |
| the production and growth characteristics of yeast and mycelial forms of candida albicans in continuous culture. | the growth characteristics of candida albicans cm145,348 have been examined under aerobic conditions in continuous culture. at different steady states the environment was controlled with respect to the concentrations of dissolved oxygen, carbon and nitrogen, the ph, and the temperature. dry matter, substrate concentration, yield, specific oxygen uptake, specific carbon dioxide release and respiration quotient were examined as a function of the dilution rate. the morphology depended on the carbon ... | 1976 | 6622 |
| antifungal properties of alpha,omega-alkanedicarboxylic acids and their dimethyl esters. | thirteen alpha, omega-alkanedicarboxylic acids (c2-c12, c14, and c16) and their dimethyl esters were tested against aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabourauc dextrose agar at ph 4.0 and 5.6. toxicity to canadida albicans, trichophyton mentagrophytes, and mucor mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. the dicarboxylic acids possessed very poor to no antifungal activity against all six fungi. the ... | 1976 | 9194 |
| approach to a practical method for screening and identifying microorganism genera from urine (author's transl). | in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ... | 1976 | 11179 |
| pigment formation by trichophyton rubrum: differentiation between t. rubrum and t. mentagrophytes on serum albumin agar (author's transl). | on serum albumin agar (bovine albumin) with initial ph-values of 5.0-6.0, trichophyton rubrum showed a yellow pigmentation, and on the same medium with an initial ph-value of 7.0, a red pigmentation. strains of trichophyton mentagrophytes have never shown pigmentation until now, the scarce growth (submerged) of t. rubrum on serum albumin agar is associated with a pigment formation that may be used for diagnostic purposes. it could be read within 3-7 days. serum albumin agar with the initial ph-v ... | 1977 | 15390 |
| neutrophil function in bone marrow transplant recipients. | the neutrophil function of seven patients receiving allogeneic bone marrow transplantion was studied. five of the patients had been transplanted for aplastic anaemia and two for acute leukaemia. determinations were made of neutrophil phagocytosis, chemotaxis, random migration, and microbicidal activity for candida albicans and staphylococcus aureus. one patient showed a decreased ability to kill c. albicans at a time when she had active pneumonia due to pneumocystis carinii. the remainder of the ... | 1977 | 15582 |
| reconstitution of t-cell function in severe combined immunodeficiency disease following transplantation of early embryonic liver cells. | in a 51/2-month-old male infant with adenosine deaminase-positive severe combined immunodeficiency disease, who had no suitable bone marrow donor, immunologic reconstitution was attempted with lymphoid cells obtained from the liver of a 4- to 5-week-old-male human embryo. a mild graft-versus-host reaction began three weeks later. t-cells, which were absent prior to infusion of hepatic lymphoid cells, rose to a maximum of 554/mm3 at 16 weeks post transplantation. a normal lymphocyte response to p ... | 1977 | 16104 |
| synthesis and biological activity of some vinyl-substituted 2-nitroimidazoles. | in previous studies 1-methyl-2-nitro-1h-imidazole-5-carboxaldehyde and 1-methyl-2-nitro-5-vinyl-1h-imidazole were found to posses interesting antimicrobial activities. we have now prepared some 2-nitro-1h-imidazoles in which the 5-vinyl chain bears selected functional groups (cho, coch3, no2) as well as nitrogen-condensation derivatives of the carbonyl functions. furthermore, 5-methyl-2-nitro-1-vinyl-1h-imidazole has been synthesized. all the compounds, and some intermediates, have been assayed ... | 1977 | 16132 |
| atopic dermatitis: an immunologic disease complex and its therapy. | | 1977 | 16516 |
| in vitro inhibition of growth of neisseria gonorrhoeae by genital microorganisms. | the ability of microorganisms present in titers of over 10(5)/ml in the vaginal or cervical secretions to inhibit growth of n. gonorrhoeae in vitro was tested. study of a strain of n. gonorrhoeae against 77 microorganisms demonstrated that most strans of s. epidermidis, s. aureus and the one "gaffkya anaerobia" interfered with the growth of the n. gonorrhoeae. a minority of strains of s. viridans, neisseria, candida and bifidobacterium demonstrated interference. no strains of enterococcus, dipth ... | 1977 | 17163 |
| isolation, purification, identification, synthesis, and kinetics of activity of the anticandidal component of allium sativum, and a hypothesis for its mode of action. | | 1977 | 19700 |
| phenothiazine phototoxicity: toxic chlorpromazine photoproducts. | the effect of preirradiated chlorpromazine (cpz) solutions has been studied in vivo in the guinea pig and in vitro in a yeast culture. with intracutaneous administration of cpz solutions preirradiated with long-wave ultraviolet radiation (uva), an inflammatory response was attained equaling the response achieved when nonirradiated solutions were injected and the skin of the animals was subsequently irradiated with uva. preirradiated cpz was not toxic to the yeast culture. during irradiation the ... | 1977 | 20478 |
| factors influencing germ tube production in candida albicans. | the capacity of candida albicans to produce germ tubes in a simple medium is analysed as a function of the ph variation, the bacterial supernatant and the addition of differing concentrations of various species of bacteria. | 1977 | 22042 |
| lysis of intact yeast cells and isolated cell walls by an inducible enzyme system of arthrobacter gjm-1. | bacterium arthrobacter gjm-1 known in the literature as a good producer of alpha-mannanase was found to accumulate in the culture fluid lytic activities against viable yeast cells during growth on isolated cell walls or beta-glucan fractions of yeast. the accumulation of the lytic activities showed an inducible character. the lytic system produced in the medium containing baker's yeast cell walls was capable of complete solubiliaztion of cell wals in vitro. the system lysed viable cells of a num ... | 1977 | 22164 |
| a32390a, a new biologically active metabolite. i. discovery and fermentation studies. | a32390a is an isonitrile-containing derivative of diacyl d-mannitol. the compound is produced in fermentation as the major component of a metabolic complex known as a32390. a32390a inhibits dopamine-beta-hydroxylase reduces heart and adrenal norepinephrine levels, lowers blood pressure in hypertensive rats, and possesses antibiotic activity vs. gram-positive bacteria and fungi, including candida albicans. a32390 is produced in submerged culture by a mold, a species of pyrenochaeta, nrrl-5786. gl ... | 1978 | 24033 |
| subcellular distribution and pyridine nucleotide requirement of hydroxysteroid dehydrogenase activities in candida albicans. | | 1978 | 24137 |
| non-steroid anti-inflammatory drugs: influence of extra-cellular ph on biodistribution and pharmacological effects. | | 1978 | 24453 |
| factors influencing the susceptibility of candida albicans to the polyenoic antibiotics nystatin and amphotericin b. | factors influencing the interaction between candida albicans and the polyenoic antibiotics nystatin and amphotericin b have been investigated using a k+-specific electrode to measure polyene-mediated efflux of cellular k+. in batch cultures, sensitivity was a function of culture age. using continuous (chemostat) cultures, the influence of growth-limiting substrate, specific growth rate, growth temperature and growth ph were examined. carbon-limited cultures showed the highest sensitivity of thos ... | 1978 | 24676 |
| antifungal properties of 2-alkynoic acids and their methyl esters. | thirteen 2-alkynoic acids and their methyl esters (c3--c12, c14, c16, and c18) were tested against aspergillus niger, trichoderma viride, and myrothecium verrucaria in sabouraud dextrose agar at ph 4.0 and 5.6. toxicity to candida albicans, trichophyton mentagrophytes, and mucro mucedo was determined in the same medium at ph 5.6 and 7.0 in the absence and presence of 10% beef serum. the fungitoxicity of the acids was influenced by chain length, ph of the medium, and absence or presence of adsorb ... | 1978 | 26458 |
| mycological profile of crew during 56-day simulated orbital flight. | mycological analyses were made on specimens obtained from the skin, oral rinse, urine, and feces of three subjects participating in a 56 day skylab simulation test. samples were taken over a 175 day period divided into three phases consisting of a prechamber, intrachamber, and postchamber period. a total of 69 species of filamentous fungi and 22 species of yeast and yeast-like fungi were recovered and identified. there was a marked decrease in the isolation incidence of both the filamentous and ... | 1978 | 29227 |
| [dehydrogenase isoenzymatic spectrum of alanine, aspartic and glutamic acids in candida albicans yeasts]. | the isoenzyme spectrum of dehydrogenases of alanine, aspartic and glutamic acids requiring nad and nadp as cofactors was studied in candida albicans. the spectrum of aspartate dehydrogenase was found to depend on a cofactor being used. the isoenzyme systems are related to proteins which posess various physico-chemical and antigenic properties. | 1978 | 30885 |
| proteolytic activity of candida albicans: action on human salivary proteins. | the susceptibility of human salivary proteins to degradation by candida albicans was studied. the organism was cultivated in either whole-salivary supernatant or parotid fluid, both of which were supplemented with glucose (0.1%). the culture ph's were at, or above, neutrality. after growth, the culture supernatant solutions were examined by polyacrylamide gel electrophoresis for alterations in their profiles of salivary proteins. no evidence of proteolysis of whole-saliva or parotid fluid protei ... | 1978 | 32141 |
| commitment to germ tube or bud formation during release from stationary phase in candida albicans. | | 1979 | 35359 |
| the antibacterial properties of sodium dichloroisocyanurate and sodium hypochlorite formulations. | | 1979 | 35510 |
| effect of ph on sporicidal and microbicidal activity of buffered mixtures of alcohol and sodium hypochlorite. | the effect of ph on the activity of buffered sodium hypochlorite solution, and a buffered methanol/sodium hypochlorite mixture, against bacillus subtilis spores was investigated. the best results, considering both sporicidal activity and stability, were achieved in the ph range 7.6--8.1. the sporicidal activity and stability of five alcohol/hypochlorite mixtures, each containing a different alcohol and buffered to ph 7.6 and of hypochlorite alone buffered to ph 7.6, were compared. the mixtures w ... | 1979 | 35554 |
| [management in pollinosis and false pollinosis]. | typical cases of pollen allergen (hayfever, allergic asthma), together with isolated non-respiratory "equivalent" manifestations (urticaria, eye conditions, headache, etc.), are easy to detect on the basis of skin tests and the clinical history. such manifestations may also occur in "false pollen allergy", related in most instances by atmospheric moulds (dematiaceae), sometimes by house dust or dermatophytes (candida albicans, trichophyton sp), by food or by a bacterial infection or allergy. a c ... | 1978 | 36022 |
| [action of levorin on amino acid transport in candida albicans]. | it was shown that suppression by levorin of the leucine transport into the cells of c. albicans was due to replacement of intracellular k+ by na+ induced by the antibiotic. the alanine transport was suppressed by levorin irrespective of the ratio of the monovalent cations concentration in the medium and inside the cell. the levorin effect on the protone escape from the cells was negligible and probably played no significant role in the mechanism of the amino acid transport suppression by the ant ... | 1979 | 36028 |
| peptones and mycological reproducibility. | the ph reactions, ultraviolet spectra and phosphorus content of solutions of a variety of commercially available peptones all indicated, predictably, considerable differences in the chemical composition of the peptones. the effects of these differences on the outcome of experiments with candida albicans grown in different peptone media was investigated. the fungus produced germ tubes equally effectively on all such media provided that the inoculum was kept to 10(6) blastospores/ml or less. howev ... | 1978 | 36674 |
| detection of experimental bacteremia and fungemia by examination of buffy coat prepared by a micromethod. | rabbits received intravenous injections of bacteria or fungi, and a comparison was made of the abilities of broth cultures, plating after dilution either in saline solution or in distilled water containing triton x-100, and buffy coat examinations to detect the organisms in heart blood. the most sensitive method was broth culture. by microscopy or subculture of buffy coat cells prepared by centrifugation of blood in microhematocrit tubes, organisms were rapidly and regularly detected when their ... | 1979 | 38662 |
| antimicrobial activity of amniotic fluid obtained vaginally. | twenty-nine amniotic fluid samples were aspirated from pregnant women at term (38-40 weeks' gestation) by tapping the bulging membrane vaginally, and their effects on escherichia coli, staphylococcus aureus and candida albicans were studied. inhibition rates were 72.41% for e coli, 68.96% for s aureus and 79.31% for c albicans. two samples (6.90%) affected only a single organism, 18 (62.07%) affected two organisms and eight (27.59%) affected all three. only one sample (3.45%) had no inhibitory e ... | 1979 | 42590 |
| acidogenic fermentation of pentose alcohols by human dental plaque microorganisms. | | 1979 | 44186 |
| statistico epidemiological study of changes in the vaginal flora of contraceptive pill users in alexandria. | a stratified random sample of 1000 women with proportionate allocation according to district of residence was taken from normal females living in alexandria, egypt, and attending family planning centers in order to understand social-pathological changes in the vaginal flora of oral contraceptive (oc) users. cases were examined over 18 months, and all cases were given a combined oc. bacteriology and ph changes in vaginal flora were determined after 18 months. results of the bacteriological exa ... | 1979 | 44312 |
| widely disseminated cutaneous candidosis in adults. | of 9 adults, 5 males, 4 females, with wide dissemination of candida albicans skin lesions, the eruption started in the groin, from which it spread to other areas in most cases. in 5 cases the disseminated lesions were papulo-pustular; the rest were erythematous-squamous. hyphae and yeast cells of c. albicans were found on direct microscopy. diabetes was present in 5 patients, lymphoma in 1, and bullous pemphigoid in another. onychia and paronychia were found in 7 patients, intertriginous lesions ... | 1979 | 44577 |
| [lysis of the yeast cell wall by helicase (author's transl)]. | | 1979 | 45817 |
| experimental hematogenous endophthalmitis caused by candida albicans. | for a rabbit model of hematogenous endophthalmitis caused by candida, 80 new zealand white rabbits were given intravenous injections of 10-5 candida albicans. two weeks after infection 88 percent of infected rabbits had ocular lesions with clinical appearance identical to that of human lesions. cultures of the chorioretina and kidneys averaged 10-4.3 and 10-3.9 organisms per g of tissue, respectively, three days after infection and remained positive for 38 days. ninety-five percent of rabbits wi ... | 1975 | 48529 |
| nonoxidative fungicidal mechanisms of mammalian granulocytes: demonstration of components with candidacidal activity in human, rabbit, and guinea pig leukocytes. | granulocytes from the peripheral blood of normal subjects and a patient with hereditary myeloperoxidase deficiency were homogenized in 0.34 m sucrose. a granule-rich fraction, prepared by sedimentation at 27,000 x g for 20 min, contained components that killed c. parapsilosis in vitro. these were extractable with 0.01 m citric acid and were shown by micropreparative polyacrylamide electrophoresis to be multiple. the candidacidal activity of these neutrophil components was heat stable and they we ... | 1975 | 49298 |
| systemic amyloidosis in mice inoculated with lyophilized candida cells. | injection of lyophilized candida albicans or c. parapsilosis cells induced amyloidosis in outbred cfw mice. the disease process was irreversible, and a significant number of animals died of systemic amyloidosis within 400 days after the last treatment. the techniques described may represent a model system for the study of systemic amyloidosis. | 1975 | 49301 |
| cell-mediated immunity in systemic lupus erythematosus: alterations with advancing age. | cell-mediated immunity (cmi) was tested in young patients with systemic lupus erythematosus (sle) (mean age 30.9 years), in elderly patients with sle (mean age 70.8 years), and in young and elderly control subjects. pre-existing cmi as evaluated by skin test response to ppd and mumps antigens and the response of peripheral blood lymphocytes in culture to the mitogen phytohemagglutinin was not significantly different in thefour groups. however, the mean area of induration to candida antigen was s ... | 1975 | 49381 |
| lymphocyte transformation in vitro in patients with immunodeficiency diseases: use in diagnosis, histocompatibility testing and monitoring treatment. | we have used lymphocyte stimulation in vitro to characterize the degree of cell-mediated immunodeficiency in different patients. the effect of treatment of patients with immunodeficiencies is illustrated by lymphocyte transformation in vitro before, during and after therapy (eg bone marrow transplantation of severe combined immunodeficiency and transfer factor treatment in wiskott-aldrich syndrome). the mixed lymphocyte culture test has been used for selection of bone marrow transplant donor for ... | 1975 | 50099 |
| leukocyte inhibitory factor (lif): production by purified human t and b lymphocytes. | | 1975 | 50357 |
| antigenic relationships between candida albicans and various yeasts as reflected by immunoglobulin-class specificity. | a group of guinea pigs was inoculated into the foot pads with a single dose of candida albicans in complete freund's adjuvant, while another group was similarly inoculated once in the foot pads but also several times intramuscularly, with candida alone. all guinea pigs were bled at different intervals after immunization and sera were separated chromatographically into igg and igm fractions. in order to study the antigenic relationships as reflected by immunoglobulin-class specificity, igg and ig ... | 1975 | 50586 |
| intercellular matrix in colonies of candida. | the histochemistry and fine structure of typical colonies of six species of candida were studied, using a total of 31 clinical isolates. the colonies consisted of viable and degenerate cells which lay in an intercellular matrix. this matrix was made up of amorphous, granular, and fibrillar components, the relative proportions and total amount of which varied from species to species. the cells of all species were surrounded by a zone of homogeneous amorphus material, which may be a highly cross-l ... | 1975 | 51019 |
| cytoplasmic antigens unique to the mycelial or yeast phase of candida albicans. | crossed immunoelectrophoresis with absorption in situ was used to distinguish the cytoplasmic antigens unique to the mycelial or yeast phase of candida albicans from cytoplasmic antigens shared by both phases. the soluble cytoplasmic extracts of each growth phase had at least six distinct antigenic constituents not shared by the other phase. this technique is recommended for the analysis of closely related antigenic complexes. | 1975 | 53205 |
| letter: allergy in the gastrointestinal tract. | | 1975 | 53758 |
| practice laboratory bacteriology. | | 1975 | 53936 |
| delayed hypersensitivity to fungal antigens in mice. ii. molecular classes in immunogenic rna extracts that transfer delayed hypersensitivity. | the transfer of delayed hypersensitivity to coccidioides immitis and candida albicans antigens with immunogenic rna extracts was studied in a mouse model. sensitivity was measured by skin tests and footpad swelling responses. immunogenic rna converted normal spleen cells in vitro so that they produced antigen-specific delayed hypersensitivity in mice that were given injections of the cells. rnase reduced the rate of, but did not abolish, in vitro interaction of immunogenic rna extracts with lymp ... | 1976 | 57198 |
| [antigenic relations between c. albicans and t. glabrata]. | | 1975 | 58584 |
| lymphocyte sensitization to aspergillus fumigatus antigens in pulmonary diseases in man. | in vitro studies of t-lymphocyte responses using five different batches of aspergillus fumigatus antigens, were undertaken in twelve patients with a. fumigatus related lung disease and in three normal controls. using a leucocyte migration method, five of the twelve patients showed significant inhibition of leucocyte migration with a migration index of 0-80 or less to a. fumigatus, but in only three was this demonstrated with more than one batch of antigen (one patient with aspergilloma and two w ... | 1976 | 59639 |
| [increase of resistance to pseudomonas aeruginosa infection in mice caused by staphylococcus aureus]. | in our study of opportunistic pathogens, we have some indication that staphylococcus aureus can increase resistance in mice against pseudomonas aeruginosa. intraperitoneal injections of sublethal doses of s. aureus had a protective effect in mice against lethal doses of p. aeruginosa, more so if living and coagulase-positive s. aureus strains were injected. this protective effect was obtained both with laboratory and freshly isolated hospital strains. the interval between these infections can be ... | 1976 | 61060 |
| immunological abnormalities in patients who had the oculomucocutaneous syndrome associated with practolol therapy. | patients with and without adverse reactions on practolol therapy showed altered immune responses. there was cutaneous anergy to candida albicans and streptokinase/streptodornase antigens and depression of lymphocyte function in vitro. anticomplementary activity and a wide range of autoantibodies were found in patients who had received practolol. | 1976 | 62263 |
| [electron microscopic demonstration of immune reactions of candida cells. i. in vitro incubation of candida albicans with anti-candida human serum]. | | 1976 | 62277 |
| [clinical and immunological study of 2 cases of systemic candidiasis]. | two cases of chronic systemic candidiasis of the infancy are presented and their immunity in vivo and in vitro is studied. the clinical course, the negative intradermal tests to candida extract and the absence of lymphoblastic transformation to the same antigen, suggest an immunological deficit of the cell mediated immunity. the positive response to intradermal test of bacterial antigens, the clinical recovery from infections different from candida albicans and the positivity of the patch test t ... | 1976 | 66059 |
| [protein and antigenic spectrum of different species of yeasts of the genus candida]. | the methods of electrophoresis in polyacrylamide gel, immunoelectrophoresis and immunoprecipitation were applied to the study of the protein and antigenic spectra of different species of candida yeasts. it was revealed that for the majority of the yeast species the prevalence of proteins of definite fractions was characteristic. c. albincans displayed a marked (in comparison with the nonpathogenic species) content of proteins of proteins of group b. the most pronounced antigenic properties in c. ... | 1976 | 66829 |
| food factors in urticaria. | | 1976 | 68072 |
| transfer of delayed hypersensitivity in mice to microbial antigens with dialyzable transfer factor. | dialyzable lawrence-type transfer factor was prepared from the spleen cells of cf1 mice inoculated with coccidioides immitis- and candida albicans-killed vaccines and with live mycobacterium tuberculosis vaccine (bcg). these preparations were shown to transfer antigen-specific cell-mediated immunity to naive mice, as measured by the delayed skin test and footpad-swelling methods. reactivity could be demonstrated when the test antigens were given 24 h after the transfer factor, but not when they ... | 1977 | 68930 |
| cellular immune responses of human milk t lymphocytes to certain environmental antigens. | | 1977 | 69346 |
| susceptibility of murine transfer factor to dimerized ribonuclease a. | dialyzable transfer factor was prepared from the spleens of cf1 mice actively sensitized with killed coccidioides immitis antigen. the transfer factor was administered to normal mice either intraperitoneally or into the hind footpads. the recipient mice were tested for reactivity to the coccidioides antigen and to candida albicans antigen by means of the footpad swelling test. the transfer factor conferred antigen-specific reactivity upon normal recipient mice when given by the intraperitoneal a ... | 1977 | 70409 |
| phenothiazine phototoxicity: an experimental study on chlorpromazine and related tricyclic drugs. | a large number of phenothiazines and chemically related tricyclic drugs have been studied with respect to their phototoxic potency. two methods were used, an in vivo technique based on the inflammatory response of the mouse tail after systemic administration of the drug plus uva irradiation, and an in vitro method based on growth inhibition of candida albicans. of 27 commercial tricyclic drugs tested in vivo, the most potent were chlorpromazine and two other chlorinated compounds, prochlorperazi ... | 1977 | 70925 |
| enumerative fluorescent vital staining of live and dead pathogenic yeast cells. | a quantitative technique is presented for differentiating live and dead yeast cells grown in culture through the use of the fluorescent dye acridine orange. the method gives results that correlate well with those of other commonly used vital staining techniques and is free of certain interpretative errors inherent in them. vital staining of yeasts with acridine orange also allows for more precise assessment of the physiological state of individual cells and the culture as a whole. the progressiv ... | 1977 | 71766 |
| [in-vivo immunogenicity of 3m kcl extracts from candida albicans]. | | 1977 | 71909 |
| ultraviolet-mediated antibiotic activity of species of compositae caused by polyacetylenic compounds. | a survey of north, central, and south american composites, and of certain polyacetylenic compounds occurring in them, confirmed that the ultraviolet-mediated antibiotic activity against candida albicans can be ascribed to the presence of particular polyacetylenes and their thiophene derivatives. leaves, stems, roots, and achenes were assayed separately. an attempt was made to relate the phototoxic activity of specific compounds to their chemical structures. most composites tested were not photot ... | 1977 | 73121 |
| [serological relationship between candida and salmonella]. | to investigate the reported relationship among certain candida and salmonella c1 group o antigen, an antigen and antiserum from c. albicans strain, isolated in our institute from patient, and another antigen and antiserum prepared from s. cholera suis strain were used. on the other hand c. melinii, which does not share any antigen with salmonella, and s. newport antigen and antiserum, sharing no antigen with c. albicans were used as negative controls. c. albicans antigen and antiserum available ... | 1977 | 73124 |
| diarrhoea caused by candida. | | 1976 | 73673 |
| an automated test for the detection of significant bacteriuria. | a fully automated technique for the rapid detection of significant bacteriuria is described. the method, which depends on the detection of bacterial adenosine triphosphate (a.t.p.) by bioluminescence, was found to have better sensitivity than a conventional cultural technique which uses a standardised loop. results were available in 30 minutes. in the 957 urine samples tested, specificity was 84.5% relative to a pour-plate technique; results were not affected by the presence of non-bacterial ele ... | 1976 | 73857 |
| miconazole-resistant candida. | | 1978 | 74535 |
| bacterial content and ionic composition of sputum in cystic fibrosis. | | 1978 | 75375 |
| occlusion, carbon dioxide, and fungal skin infections. | occlusion of the skin renders it susceptible to acute fungal skin infections (dermatophytosis and candidiasis). occlusion also raised carbon-dioxide (co2) tensions at the skin's surface. comparable co2 tnesions have a pronounced effect on the morphology and metabolism of dermatophytes in vitro. it is postulated that dermatophyte conida and hyphae produce infective units under conditions of raised co2 tensions, and that occlusion of the skin produces the concentrations of co2 required for the con ... | 1978 | 75398 |
| evaluation of the antigen specific to the mycelial phase of candida albicans in the serodiagnosis of candidiasis. | we demonstrated 2 different antibodies against candida albicans in patients' sera, the detection rates were proportional to the severity of candidiasis. one antibody was directed toward antigen shared by candida in both blastospore and mycelial phases and the other was directed against antigen found only in the mycelial phase of candida. the presence of the latter may reflect the invasive form of candidiasis. | 1977 | 75594 |
| gastritis and cimetidine: a possible explanation. | | 1978 | 77388 |
| comparative immunochemical studies of different antigens of deep mycosis pathogens. | the disintegration of cells of coccidioides immitis, histoplasma capsulatum, blastomyces dermatitidis and candida albicans by ultrasonical treatment, repeated freezing-thawing or mechanical methods permitted to obtain antigens with a high protein content. the treatment of these fungal cells with beta-naphthol, ethanol, glycerine or alkali makes it possible to isolate antigens containing mainly polysaccharides. the studied antigens turned out to be complex compounds. proteins, glycoproteins, nucl ... | 1978 | 79329 |
| [phagocytic granulocyte function in hemoblastoses]. | judging from the spontaneous nbt reduction, the indices of phagocytosis and nbt, there is a moderate, but statistically significant diminution of these parameters in leukaemia and malignant lymphoma including plasmocytoma. moreover, further diminutions could be identified during the acute stage of the disease (first diagnosis or recidive) in acute leukaemia and lymphogranulomatosis, but not for chronic myeloic leukaemia. | 1978 | 79520 |
| [differential spectrophotometric method of analysing levorin in the culture broth and in the mycelium]. | it was shown on model experiments that the microbiological method was not applicable for determination of levorin content in industrial intermediate products containing in addition levoristatin, since the presence of the latter made higher the results of the microbiological assay. because of this till to the present date the quantitative content of levorin in the industrial intermediate products was determined photometrically using alcohol (pure solvent) as the reference solution. still, this me ... | 1978 | 81654 |
| stomach as source of bacteria colonising respiratory tract during artificial ventilation. | 10 adults had intermittent positive-pressure ventilation for 3--34 days. all 10 had paralytic ileus. microbial overgrowth in the stomach was found in 9 patients (bacterial in 7 and fungal in 2); gram-negative bacteria predominated. the trachea invariably became colonised by bacteria, mainly gram-negative organisms. in 3 instances the gram-negative bacteria were found in the stomach before they appeared in the tracheal aspirate, and in 1 case the pathogen originated in the faeces. | 1978 | 81991 |
| cross-reaction between the mycelial galactomannas of three hormodendrum strains and the mannans of two candida albicans strains of different serotypes, a and b. | cross-reactivity between galactomannans of three representative hormodendrum strains, h. pedrosoi ifo 6071, h. compactum ifo 6726, and h. dermatitidis ifo 6421, and the mannans of two candida strains, c. albicans nih a-207 (serotype a) and c. albicans nih b-792 (serotype b), were investigated by means of agar-gel double diffusion, quantitative preciptin reaction, and passive cutaneous anaphylaxis. the following results were obtained. (i) antisera to whole cells of three hormodendrum strains were ... | 1979 | 84792 |
| impaired monocyte function in liver cirrhosis. | | 1979 | 84985 |
| septal ultrastructure in candida albicans. | fine structural studies on the septa of candida albicans in vitro (when leading a saprophytic existence) and the organism in its invasive form (as a pathogen in oral candidosis) have shown that in the former the septum exhibits a unique central perforation resembling an aggregate of fine canaliculi connecting one cell to the other. in the invasive form the septum is non-perforated and appears as a solid structure. septal ultrastructure is well characterised in many pathogenic fungi. our observat ... | 1978 | 86287 |
| antibodies to germinating and yeast cells of candida albicans in human and rabbit sera. | two major antigenic components, i and ii, were detected by double immunodiffusion in sonic extracts of the germinating (g) or yeast (y) cells of the dimorphis organism, candida albicans group a. component i may be a heterogeneous mixture of antigens which are stable to heating and phenol. component ii is more homogeneous but is labile to heat and phenol. rabbit antisera, showing only precipitin to component ii or certain human sera at high dilution, were found to react with g cells to give an im ... | 1979 | 87404 |
| the proliferative response of human lymphocytes to antigen is suppressed preferentially by lymphocytes precultured with the same antigen. | human blood lymphocytes activated in vitro with antigen to which the donor is reactive are capable of suppressing the secondary proliferative response of autochthonous fresh cells to antigen. both antigen-specific and antigen-nonspecific suppression can be detected in each experiment. these suppressor cells act by decreasing the number of lymphocytes entering the proliferative response rather than by slowing or otherwise inhibiting ongoing proliferation. the suppressor cells must be added soon a ... | 1979 | 87445 |
| histamine-releasing activity (hra). i. production by mitogen- or antigen-stimulated human mononuclear cells. | supernatants from 1- to 2-day cultures of human mononuclear cells induced the release of histamine from basophils. generation of this histamine-releasing activity (hra) was stimulated by addition of concanavalin a to the cell cultures. mononuclear cells were also cultured with sksd and candida albicans antigens. stimulation of hra production by these antigens was correlated with positive delayed skin reactions. serial dilutions of supernatants assayed for hra provided a semiquantitative determin ... | 1979 | 88479 |
| diagnostic dilemma of a 13-month-old boy with 'late-onset' combined immunodeficiency. | a 13-month-old boy had a "late-onset" form of combined immunodeficiency and a fulminant pneumocystis carinii pneumonia of one month's duration. there was no evidence of cutaneous-delayed hypersensitivity responses to diphtheria-tetanus toxoids, candida albicans, or streptokinase-streptodornase, or of lymphocyte dna synthesis after in vitro stimulation with the mitogens phytohemagglutinin and concanavalin a, and only 2% to 4% of peripheral blood e-rosetted t lymphocytes. the serum igm level was n ... | 1979 | 88898 |
| autoregulation of germ tube formation by candida albicans. | germ tube formation by candida albicans is at least partially controlled by a product(s) of the yeast phase of the organism which is released from cells upon incubation at 37 degrees c in tissue culture medium or fetal calf serum. this germination regulatory substance is stable under conditions of lyophilization and heating of 70 degrees c, but becomes inactivated at ph values of 4.0 and 9.5. a germination regulatory substance was produced by both strains of c. albicans tested and by a strain of ... | 1979 | 89087 |
| [use of the "ingrowth tubes" diagnostic test in candidiasis]. | | 1979 | 89236 |
| the isolation of an optimal small size immunoferritin conjugate fraction. | a procedure is described for preparing immunoferritin conjugates consisting of one antibody molecule attached to one ferritin molecule. results obtained with such a reagent faithfully reflect the true distribution of antigenic determinants, and allow more accurate quantification. | 1979 | 91648 |
| [microstix-candida test as an attempt at accelerating and facilitating mycological laboratory diagnosis of foot mycoses]. | | 1979 | 92017 |
| viability assessment by dye exclusion. a fluorescent method for fungal cells. | a new fluorescent staining technique for fungi utilizes the differential affinity of dead cells for rhodamine b. a mounting medium has been devised that includes the dye. this medium allows direct processing of cells from a broth and provides optimal conditions for fluorescence intensity. comparison with the standard methylene blue exclusion test as applied to the candida albicans yeast phase indicates similar specificity. various pseudohyphae and several fungi also exhibited selective uptake of ... | 1979 | 92216 |
| a simple method for staining semi-thick tissue sections to demonstrate fungal elements. | a simple method of staining epon embedded semi-thick sections with schiff-reagent is described. this technique allows fungal elements in tissue to be readily observed. the combination of schiff-reagent with a methylene blue counterstain for demonstrating fungal elements and tissue structures is also described. | 1979 | 93702 |
| inhibition of ergosterol synthesis in candida albicans by ketoconazole [proceedings]. | | 1979 | 93943 |
| [specific soluble cell-wall antigens of "candida tropicalis" demonstrated by crossed immunoelectrophoresis (author's transl)]. | in the present study, soluble cytoplasmic extracts of candida albicans and c. tropicalis were prepared and showed respectively 67 and 47 precipitates in crossed immunoelectrophoresis performed with a commerical goat anti-c. albicans immunserum. purified cell walls of c. tropicalis have been prepared, and corresponding antiserum was got in rabbits. although important cross-reacting antigens exist between soluble cytoplasmic extracts of c. albicans and c. tropicalis, the c. tropicalis cell wall an ... | 1979 | 94248 |
| immunodiagnosis of systemic candidiasis: mannan antigenemia detected by radioimmunoassay in experimental and human infections. | a radioimmunoassay (ria) that detects candida mannan was developed so that immunodiagnosis of systemic candidiasis could be improved. the ria was evaluated in an animal model of disseminated disease and in a panel of patient sera. mannan antigenemia was detected with the ria in 52% of 29 rabbits with systemic candidasis, but not in 60 normal rabbits or 31 rabbits with systemic aspergillosis. in an evaluation of human sera, mannan antigenemia was detected in five of 11 patients with systemic cand ... | 1979 | 94341 |
| [cross reactions of eight yeasts and their importance in serological candida diagnostic (author's transl)]. | while the antigen structure of yeast cell walls is well known, the immunological cross reactivity of these is often difficult to interpret. the cross reactions of eight yeasts were tested in rabbit hyperimmune sera by the indirect immunofluorescence test. among the eight species examined were the medically important yeasts c. albicans, c. pseudotropicalis, c. krusei, c. parapsilosis, c. tropicalis, t. glabrata, and c. guilliermondii and the apathogenic yeast s. cerevisiae. it has been shown, tha ... | 1979 | 94650 |
| effects of ascorbate on leucocytes: part ii. effects of ascorbic acid and calcium and sodium ascorbate on neutrophil phagocytosis and post-phagocytic metabolic activity. | the effects of ascorbic acid and calcium and sodium ascorbate at a concentration range of 10(-6)m - 10(-1)m on polymorphonuclear leucocyte (pmn) phagocytosis of candida albicans and post-phagocytic nitroblue tetrazolium (nbt) reduction, hexose monophosphate shunt (hms) activity and myeloperoxidase-mediated iodination of ingested protein were investigated. phagocytosis of c. albicans was unaffected by ascorbate concentrations of 10(-6)m - 10(-2)m; however, progressive inhibition was observed at c ... | 1979 | 95138 |
| cell-mediated immunity to pityrosporum orbiculare in tinea versicolor. | pityrosporum orbiculare, the presumed etiologic agent of tinea versicolor, was cultured in vitro and antigenic extracts prepared from the cultured organisms. studies with lymphocytes from human cord blood and peripheral blood of guinea pigs demonstrated that such extracts were not mitogenic. further studies in guinea pigs indicated that the animals could be sensitized by the injection of p. orbiculare extract in freund's complete adjuvant and that this extract could elicit lymphocyte transformat ... | 1978 | 96143 |
| antimicrobial activity of some fatty acid derivatives. | | 1978 | 96164 |
| methods for storage of antimicrobial effectiveness test inoculum suspensions below freezing. | studies were conducted to determine the suitability of storage below freezing of some antimicrobial effectiveness test inoculum organisms: staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and candida albicans. several solutions used for protecting microorganisms subjected to storage below freezing were compared. comparison of 10% dextrose with other solutions (distilled water; 0.07 m phosphate buffer, h 7.0, with 15% glycerol; and 0.07 m phosphate buffer, ph 7.0, with 7.5% dimeth ... | 1978 | 96247 |
| the occurrence of candida in hospital patients and normal subjects. | the distribution of different candida species and different serogroups of c. albicans have been analysed. c. albicans is by far the most predominant species isolated from all clinical specimens. group a of c. albicans was isolated 10 times more frequently than group b strains from patients who had respiratory infection and 4.4 times more frequently from those who had other clinical conditions. however, both serogroups were isolated at comparable frequencies from the genitals. in the instances wh ... | 1977 | 96537 |