| fluorescent staining of fungal nuclei with a benzimidazol derivative. | a direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. the fluorochrome is a benzimidazol derivative (33258 hoechst) known to bind selectively to deoxyribonucleic acid at neutral ph. the advantages of 33258 hoechst relative to feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, the absence of fading or rapid quenching of the fluorescence, and the omission of acid hydrolysis from th ... | 1978 | 75211 |
| synthesis of gamma-l-glutaminyl-[3,5-3h]4-hydroxybenzene and the study of reactions catalyzed by the tyrosinase of agaricus bisporus. | gamma-l-glutaminyl-[3,5-3h]4-hydroxybenzene was synthesized in order to study the kinetics of its hydroxylation by tyrosinase purified from agaricus bisporus and to explore its role in the induction of the dormant state in the spores of this species. it was found to be unique among the monophenolic substrates for tyrosinase in that the lag period for the hydroxylation reaction decreased with increasing substrate concentration. unlike previously studied compounds, this phenol appeared to function ... | 1979 | 115880 |
| tumorigenic effect of 4-methylphenylhydrazine hydrochloride in swiss mice. | 4-methylphenylhydrazine hydrochloride was administered as 10 weekly subcutaneous injections of 140 microgram/g body weight and as 7 weekly intragastric instillations of 250 microgram/g body weight in physiological saline to randomly bred swiss mice. treatments given subcutaneously resulted in induction of lung tumors in incidences of 36% in females and 44% in males, while intragastric treatment caused a 40% incidence in females. in addition, it gave rise to blood vessel tumors by intragastric ro ... | 1977 | 143810 |
| surface alterations in calf lymphocytes oxidized by sodium periodate. | in order to investigate alterations in surface structure in transformed lymphocytes, calf submandibular lymph node cell suspensions were oxidized with naio4. oxidezed lymphocytes were morphologically transformed and had higher rates of dna synthesis by 2 days after treatment. these results were prevented by reduction of the cell suspension with nabh4, or by neuraminidase treatment of cells prior to oxidation. the amount of 125i-labeled agaricus bisporus lectin bound to cells immediately after ox ... | 1976 | 178647 |
| carbohydrate composition of peripheral, cultured and leukaemic human lymphocyte plasma membranes. | plasma membranes isolated from peripheral blood lymphocytes of normal donors, lymphocytes from patients with chronic lymphatic leukaemia (cll), a t cell and b cell line (molt-3 and rpmi-1788) were analysed and compared for total carbohydrate contents. t cells and peripheral blood lymphocytes contained the highest relative amounts of sialic acid and fucose, whereas chronic lymphatic leukaemic cells possessed the highest amounts of n-acetylgalactosamine and also more total cell surface carbohydrat ... | 1978 | 206031 |
| structure of the oligosaccharides of three glycopeptides from calf thymocyte plasma membranes. | the carbohydrate composition and oligosaccharide structure of three glycopeptides isolated from delipidated calf thymocyte plasma membranes following pronase digestion have been determined. five major glycopeptide fractions were separated using bio-gel p-6 gel filtration and diethylaminoethylcellulose chromatography. the structure of the oligosaccharide chains of three of these glycopeptides was determined by a combination of sequential degradation with glycosidases and methylation analysis. the ... | 1978 | 306261 |
| a seven-day volumetric spore trap for use within buildings. | the burkard volumetric spore trap, designed to operate for seven days continuously in the field, was modified to sample still air within buildings. the efficiency with which spores of lycopodium clavatum and agaricus bisporus were trapped at two rates of suction was determined. spore distribution within traces and deposition on surfaces not beneath the orifice were assessed. in an appendix catches of four spore types by the hirst and burkard (field model) spore traps operating over mown grass we ... | 1978 | 375094 |
| gamma-l-glutaminyl-4-hydroxybenzene, an inducer of cryptobiosis in agaricus bisporus and a source of specific metabolic inhibitors for melanogenic cells. | a stable phenol, gamma-l-glutaminyl-4-hydroxybenzene (ghb), is oxidized by tyrosinase in the gill tissues of the mushroom agaricus bisporus to a quinone and a second oxidation product which together suppress mitochondrial energy production and the synthesis of proteins and nucleic acids in the zygote, thus establishing dormancy in the spores. brief incubation of cultured murine l1210 leukemia and b-16 melanoma cells with mum concentrations of the purified quinone notably prolonged survival times ... | 1977 | 403000 |
| new lectin receptors in carcinoembryonic antigen (cea). | the glycoprotein cea (carcinoembryonic antigen) carries carbohydrate groups, which react with the plant lectins from agaricus bisporus, arachis hypogaea (peanut), with tridacnin from invertebrate clams and with the anti-a lectins from snails. accordingly, it has cryptantigenic structures, which correspond to the t or t-like antigen, the tridacnin receptor and to the so called a-like antigen. | 1978 | 414933 |
| syntheses of o-beta-d-galactopyranosyl-(1 leads to 3)-0-(2-acetamido-2-deoxy-alpha(and -beta)-d-galactopyranosyl)-n-tosyl-l-serine and their interaction with d-galactose-binding lectins. | in order to use, as hapten inhibitors against various galactose-binding lectins, the derivatives of o-beta-d-galactopyranosyl-(1 leads to 3)-o-(2-acetamido-2-deoxy-alpha-d-galactopyranosyl)-l-serine, which is the common core structure of sugar chains of most mucins, the synthesis of these compounds was investigated. koenigs-knorr condensation of the 4,6-o-benzylidene derivative of o-(2-acetamido-2-deoxy-alpha-d-galactopyranosyl)-n-tosyl-l-serine methyl ester with 2,3,4,6-tetra-o-acetyl-alpha-d-g ... | 1979 | 427833 |
| basidiospore numbers in agaricus bisporus (lange) imbach. | the number of spores per basidium in the cultivated mushroom agaricus bisporus can be readily determined using the light microscope. | 1977 | 556614 |
| [cadmium uptake by mushrooms (author's transl)]. | mushrooms of the subgenus rubescentes (for example agaricus bisporus and agaricus campestris) accumulate for less cadmium than most species of the subgenus flavescentes (for example agaricus arvensis and agaricus silvicola). hence it appears there is no danger for human health, from too much cadmium, when eating the often gathered agaricus campestris. the caps of the mushrooms enrich cadmium to twice the amount present in the stems. the authors analysed 32 samples of wild mushrooms of the subgen ... | 1977 | 562042 |
| ethylene production by axenic fruiting cultures of agaricus bisporus. | axenic cultures of agaricus bisporus were used to show that the rise in ethylene production during fruiting derives from its own metabolism. | 1977 | 562129 |
| tumor induction with the n'-acetyl derivative of 4-hydroxymethyl-phenylhydrazine, a metabolite of agaritine of agaricus bisporus. | n'-acetyl-4-(hydroxymethyl)phenylhydrazine was administered as a 0.0625% solution in drinking water continuously for the life span of swiss mice, from 6 weeks of age. compared to that in untreated controls, in treated animals the lung tumor incidence rose from 15 to 34% in females and 22 to 48% in males, whereas the incidence of blood vessel tumors increased from 8 to 32% in females and from 5 to 30% in males. histopathologically, the tumors were classified as adenomas and adenocarcinomas of the ... | 1978 | 563287 |
| the insensitivity of mushroom nuclear rna polymerase activity to inhibition by amatoxins. | nuclear fractions isolated from amanita phalloides, amanita muscaria and agaricus bisporus were subjected to in vitro rna synthesis assays in the presence of various concentrations of amatoxins. the mushroom nuclei were highly insensitive to inhibition by amatoxin when compared to assays of nuclear fractions isolated from the oömycete fungus, achlya ambisexualis and from rabbit brain. | 1978 | 567964 |
| the binding of lectins to components of plasma membranes from porcine submaxillary lymph node lymphocytes. | by sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis the plasma membranes from porcine lymphocytes contain at least 30--35 glycopolypeptides and one or more glycolipids to which one or more of 12 purified lectins bind. the specificities of binding generally followed the same pattern as those of the reaction of the lectin with intact pig lymphocytes. some lectins (e.g., the isolectin pair, agaricus bisporus lectins a and b and a group consisting of the lens culinaris a and b isol ... | 1978 | 568485 |
| [viruses of the cultivated mushroom (agaricus bisporus (j. lge) imbach.)]. | | 1978 | 569245 |
| purification and characterization of rna polymerase ii resistant to alpha-amanitin from the mushroom agaricus bisporus. | the dna-dependent rna polymerases ii or b (ribonucleosidetriphosphate:rna nucleotidyltransferase, ec 2.7.7.6) from the mushroom agaricus bisporus has been purified to apparent homogeneity. the purification procedures involve precipitation with polyethylenimine, selective elution of rna polymerase ii from the polyethylenimine precipitate, ammonium sulfate fractionation, deae-cellulose chromatography, cm-cellulose chromatography, and exclusion chromatography on bio-gel a-1.5m. with this procedure ... | 1979 | 570413 |
| studies of the spore walls of agaricus bisporus and agaricus campestris. | the composition and ultrastructure of spore walls of agaricus bisporus and agaricus campestris were compared by chemical and enzymatic assays and electron microscopy. polymers of n-acetylglucosamine (chitin) and glucosamine (chitosan) were the major carbohydrate wall components of both species. the chitin to chitosan ratio in the spore walls of a. bisporus was about 0.38, while in a. campestris it was about 2.8 these polymers were associated with proteins, lipids (readily extractable and bound), ... | 1979 | 570877 |
| hepatocarcinogenesis by hydrazine mycotoxins of edible mushrooms. | two edible mushrooms are known to contain hydrazine analogs. the wild false morel gyromitra esculenta contains up to 0.3% acetaldehyde methylformylhydrazone and n-methyl-n-formylhydrazine (mfh). the latter chemical, under certain conditions, also yields methylhydrazine. the commonly eaten, cultivated mushroom agaricus bisporus contains up to 0.04% beta-n-[gamma-l(+)-glutamyl]-4-hydroxymethylphenylhydrazine and 4-hydroxymethylphenylhydrazine. mfh was administered in drinking water continuously fo ... | 1979 | 572876 |
| [uptake of lead, cadmium and mercury by cultivated mushrooms]. | it is reported of the uptake of lead, cadmium and mercury by cultivated mushrooms (agaricus bisporus) from lead, cadmium and mercury-containing substrates. cd and hg were observed to accumulate significantly via the mycelium, whereas pb accumulated scarely, even if the pb contents in the substrate were high. high lead concentrations in wild mushrooms, therefore, are likely to be mainly due to emissions; whereas, with cd and hg, uptake from the soil must also be considered. | 1979 | 574616 |
| [use of gas chromatography for determination of amino acid composition of bovine serum albumin and champignons (agaricus bisporus)]. | | 1979 | 575575 |
| immunochemical detection of the thomsen-friedenreich antigen (t-antigen) on platelet plasma membranes. | platelet plasma membranes were found to possess the disaccharide beta-d-galactosyl (1-3)-n-acetyl-d-galactosamine which was measured by gas chromatography after release by alkaline borohyride treatment and desialylation. immunological evidence using the specific lectins from arachis hypogoea and agaricus bisporus and an anti-t serum confirmed the presence of this disaccharide, the immunodominant group of the thomsen-friedenreich antigen (t-antigen). this receptor was only found after prior neura ... | 1978 | 580492 |
| immuno-chemical studies on the alkali-labile carbohydrate chains of human serum glycoproteins. | human serum glycoproteins can be classified into those containing n-acetyl-d-galactosamine and into those lacking this hexosamine. the n-acetyl-d-galactosamine-containing serum glycoproteins have alkali-labile chains containing this hexosamine linked o-glycosidically to hydroxy amino acids. these alkali-labile chains can be demonstrated in neuraminic acid free serum glycoproteins by gas liquid chromatography and by using precipitating lectins from invertebrates and plants. they are represented b ... | 1979 | 762504 |
| histochemical lectin affinity technique by means of fitc-labeled serum protein fractions. | a two-step affinity technique is described for light microscopic demonstration of the concanavalin a, agaricus bisporus lectin and ricinus communis lectin binding sites by means of various fitc-labeled human and rabbit serum protein fractions. experiments for the visualization of the lens culinaris lectin and the pisum sativum lectin binding sites gaves negative results. the technique consist of two reaction steps which involve the incubation of tissue sections in the lectins followed by the vis ... | 1976 | 791903 |
| cytostatic, cytocidal and potential antitumor properties of a class of quinoid compounds, initiators of the dormant state in the spores of agaricus bisporus. | evidence indicates that dormancy is initiated in the spores of agaricus bisporus by two quinoid compounds that appear in the zygote during the prodromal period of sporulation. both are derivatives of a phenol, gamma-l-glutaminyl-4-hydroxybenzene. when purified, these quinoids specifically inhibit mitochondrial respiratory enzymes and protein synthesis in the mushroom and have comparable effects with rat liver mitochondria and ribosomes, with intact bacteria, and with bacterial ribosomes and rna ... | 1975 | 803242 |
| production of ethylene and other volatiles and changes in cellulase and laccase activities during the life cycle of the cultivated mushroom, agaricus bisporus. | nine volatile hydrocarbons, as well as methyl chloride, carbonyl sulphide and carbon disulphide, have been identified by mass spectrometry as products of agaricus bisporus in the compost used in comerical mushroom beds. of these, only ethylene showed a pattern of production that could be correlated with developmental phases of the crop, high levels being produced whenever fruit bodies were rapidly enlarging. in laboratory flask cultures, under controlled conditions, high levels of ethylene occur ... | 1975 | 811760 |
| platelet glycocalicin. ii. purification and characterization. | glycocalicin, a major glycoprotein of the platelet glycocalyx, is obtained in soluble forms following platelet homogenization and has been purified to homogeneity. glycalicin has a molecular weight of 148,000 (+/- 5,000) as determined by gel electrophoresis. it contains 60 grams % carbohydrate (46.6 mol %) comprising galactose, n-acetylgalactosamine, n-acetylglucosamine, and sialic acid as its principal sugars in a ratio of 2:1:1:2, but with a small amount of glucose (2.3 mol %), mannose (1.2 mo ... | 1976 | 823155 |
| inhibition of dna polymerase from l1210 murine leukemia by a sulfhydryl reagent from agaricus bisporus. | the 490 quinone, a natural sulfhydryl-arylating reagent from the mushroom, agaricus bisporus, markedly inhibited l1210 murine leukemia dna polymerase alpha while resulting in little inhibition of dna polymerase beta from this source. this quinone was more strongly inhibitory than p-chloromercuri-benzoate or n-ethylmaleimide and was less readily neutralized by sulfhydryl-containing molecules such as dithioerythritol. preliminary experiments indicate that dna protects dna polymerase alpha from inh ... | 1977 | 832267 |
| uptake of benomyl by the cultivated mushroom, agaricus bisporus. | | 1976 | 943434 |
| study on development of agaricus bisporus by fluorescent microscopy and scanning electron microscopy. | two strains of agaricus bisporus have been investigated by fluorescent microscopy and scanning electron microscopy. somatic nuclei, stained with auramin o and examined by fluorescent microscopy, appear to be randomly distributed, divide asynchronously, and assume a nonclassical or "two-track" configuration during mitotic metaphase. a similar configuration has been observed for nuclei during meiosis, but early meiosis in a. bisporus appears to be classical, usually with nine pairs of chromosomes ... | 1976 | 944181 |
| carbohydrate metabolism in agaricus bisporus (lange) sing: changes in soluble carbohydrates during growth of mycelium and sporophore. | changes in the ethanol-soluble carbohydrate content of agaricus bisporus mycelium and sporophores grown on semi-defined media and commercial compost were studied. the accumulation of mannitol in the sporophore during its growth was not accompanied by an increase in mycelial mannitol. the other major soluble carbohydrate of the sporophore, trehalose, decreased throughout the growth of the sporophore; a parallel decrease was observed in the mycelium. the main accumulation of mannitol was in the pi ... | 1976 | 945325 |
| double-stranded ribonucleic acid in agaricus bisporus. | double-stranded ribonucleic acid present in virus-infected mushrooms of agaricus bisporus has been resolved through polyacrylamide gel electrophoresis into six molecular-weight forms. identification of these six double-stranded ribonucleic acids in mushrooms by this procedure has proven to be a useful and diagnostic method for viral infection in the cultivated mushroom. | 1976 | 945716 |
| studies on mushroom flavours. 3. some flavour compounds in fresh, canned and dried edible mushrooms. | the concentrations of 1-octen-3-ol, 5'-gmp and glutamic acid were compared in 8 fresh, 3 canned and 5 dried mushroom species. the highest amounts of 1-octen-3-ol and 5'-gmp were found in the fresh muschrooms. agaricus bitorquis, pleurotus ostreatus and pholiota squarrosa contained 5-7 times as much 1-octen-3-ol as agaricus bisporus and calvatia gigantea 58 times as much. coprinus comatus and pleurotus ostreatus contained much 5'-gmp. little 1-octen-3-ol and 5'-gmp were found in most dried and ca ... | 1976 | 987665 |
| [studies on mushroom flavours. 1. organoleptic significance of constituents of the cultivated mushroom, agaricus bisporus]. | the flavour of agaricus bisporus was studied by comparing the results obtained in chemical and organoleptic analysis of the same extract. the natural (-)-form of 1-octen-3-ol had a stronger flavour than the (+)-form and was the most important aroma component. nucleotides, amino-acids and carbohydrates also contributed significantly. benzaldehyde, benzyl alcohol, 1-octen-3-one, n-butyric and isovaleric acids as well as low-boiling volatiles contributed less to the flavour. a synergistic effect of ... | 1976 | 988686 |
| effect of lectins on hormone release from isolated rat islets of langerhans. | lectins from agaricus bisporus and agaricus campestris stimulate insulin and glucagon release from isolated rat islets in the presence of 2 mm glucose. in the case of insulin release, maximal stimulation was observed at lectin concentrations above 58 mug. per milliliter (approximately 1 mum).a. bisporus pha-b-stimulated insulin release was independent of a source of metabolic energy but was abolished by deuterium oxide. the lectin did not alter islet glucose oxidation to co2 or incorporation of ... | 1975 | 1098948 |
| growth potential of clostridium botulinum in fresh mushrooms packaged in semipermeable plastic film. | fresh mushrooms (agaricus bisporus) were inoculated in the stem, gill, or cap with clostridium botulinum spores. they were placed with uninoculated mushrooms in paper board trays, which were then covered and sealed in a polyvinyl chloride stretch film to simulate prepackaged mushrooms available at retail stores. when incubated at 20 c, botulinum toxin could be detected as early as day 3, or 4, when the mushrooms still appear edible. mushrooms inoculated in the stem with 1,000 type a spores freq ... | 1975 | 1108793 |
| localization of the binding sites for the ricinus communis, agaricus bisporus and wheat germ lectins on human erythrocyte membranes. | freeze-etch electron microscopy has been utilized to localize the binding sites for the ricinus communis, agaricus bisporus and wheat germ lectins on human erythrocyte membranes and to determine the relation of these different glycoprotein receptors to the intramembranous particles. a. bisporus lectin, which could be visualized directly on the surface of erythrocyte membranes, and ferritin conjugates of wheat germ agglutinin showed a distribution that correlates exactly with the intramembranous ... | 1975 | 1148231 |
| concerning the role of mitochondria in cryptobiosis. | the metabolic characteristics of the mitochondria of agaricus bisporus are altered in the zygote by specific inhibitors that permit them to retain structural integrity in the dormant spore and enable them to initiate energy production, with apparent protein synthesis and replication during the initial phase of germination. the insensitivity of the earliest events of germination to selective cytoplasmic and nuclear inhibitors characterizes this as a transient period of unusual mitochondrial auton ... | 1975 | 1163641 |
| temperature, time, and the influence of volatiles on phialospore germination in verticillium malthosei ware. | phialospores of verticillium malthousei ware were atomized onto acclimatized water agar and incubated for 3, 6, 9, 12, and 24 hr at 12 to 36 degrees c intervals. germination occurred at all temperatures exclusive of 36 degrees c with maximum germination (90-98%) occurring in 24 hr at 12 degrees c, 12 degrees c, 12 hr at 18 degrees c, 9 hr at 24 degrees and 30 degrees c. volatiles from: 1) horse manure compost colonized by agaricus bisporus; 2) mycellium impregnated casing (soil); or 3) casing su ... | 1975 | 1170495 |
| the lipids of agaricus bisporus. | a comparison of the lipid composition of the vegetative and reproductive stages of agaricus bisporus revealed no major qualitative differences, although quantitative divergence exist. the glycolipids consisted of acylglucoses, acylmannitol, acyltrehalose and a glucosyloxyfatty acid. two of the acylglucoses corresponded to a tetra-acylglucose and to either a di- or a triacylglucose. the phospholipids were distinctive in that phosphatidylcholine could not be detected. phosphatidylethanolamine and ... | 1975 | 1176953 |
| synthetic and naturally occurring hydrazines as possible cancer causative agents. | the various synthetic substituted hydrazines, which cause tumors in animals, are briefly enumerated. to date, 19 of them have proved to be tumorigenic in animals. a number of these chemicals are found today in the environment, in industry, in agriculture, and in medicine, and the human population is exposed to a certain degree to some of them. hydrazine also occurs in nature in tobacco and tobacco smoke. the three other naturally occurring hydrazine compounds are n-methyl-n-formylhydrazine, whic ... | 1975 | 1238167 |
| lysosomes in the cultivated mushroom (psalliota bispora quel.). | fungine lysosomes are difficult to identify because some vesicular structures are present in hyphae, such as provacuoles, lomasome precursor, peroxisomes and spherosomes. moreover, many hymenomycetes, as the common cultivated mushroom (psalliota bispora quel.), lack tipical golgi apparatuses, making ontogenesis of lysosomes a matter of difficult interpretation. as shown before (scannerini, 1969) the hyphae of this fungus contain vesicles that reveal acid glycerophosphatase activity, thus we stud ... | 1975 | 1239397 |
| preparation and characterization of monoclonal antibodies against 4-aminobenzoate hydroxylase from agaricus bisporus. | a monoclonal antibody (mab, a) recognizing the fad-binding domain of 4-aminobenzoate hydroxylase (4-aminobenzoate, nad(p)h:oxygen oxidoreductase (1-hydroxylating, decarboxylating), ec 1.14.13.27) from agaricus bisporus, a common edible mushroom, had been produced (tsuji, h., ogawa, t., bando, n., kimoto, m. and sasaoka, k. (1990) j. biol. chem. 265, 16064-16067). in the present study, three other mabs (b1, b2 and b3) against the enzyme have been further prepared in order to facilitate the struct ... | 1992 | 1371228 |
| isolation and characterization of a cellulose-growth-specific gene from agaricus bisporus. | the edible basidiomycete, agaricus bisporus, produces extracellular endoglucanase. endoglucanase production is induced by cellulose and repressed by fructose in a. bisporus grown on minimal medium, and is regulated in activity during fruiting body development. an anti-endoglucanase antibody was used to isolate cellulase-related genes. three main polypeptides of 38, 58, and 60 kda were immunoprecipitated by the antibody from products of in vitro cell-free translation of mrnas isolated from cellul ... | 1992 | 1398098 |
| mushroom worker's lung. detection of antibodies against shii-take (lentinus edodes) spore antigens in shii-take workers. | indoor cultivation of the edible mushroom shii-take (lentinus edodes) regularly leads to symptoms of mushroom worker's lung (mwl) in workers. an immunologic test is described allowing detection of igg type antibodies against shii-take spore antigens. it was found that mwl patients employed in shii-take picking (n = 5) have significantly increased antibody titres against shii-take spore antigens. different control groups, viz, mwl patients employed in the cultivation of the white button mushroom ... | 1992 | 1432300 |
| dna amplification polymorphisms of the cultivated mushroom agaricus bisporus. | single 10-bp primers were used to generate random amplified polymorphic dna (rapd) markers from commercial and wild strains of the cultivated mushroom agaricus bisporus via the polymerase chain reaction. of 20 primers tested, 19 amplified a. bisporus dna, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0 kbp. rapd markers identified seven distinct genotypes among eight heterokaryotic strains; two of the commercial strains were shown to be related to each other through single-spore ... | 1992 | 1444410 |
| siderophore-mediated iron (iii) transport in the mycelia of the cultivated fungus, agaricus bisporus. | three structurally diverse iron (iii) sequestering compounds (siderophores) were isolated from the supernatants of early stationary phase iron-deficient cultures of vegetative mycelia of the cultivated mushroom, agaricus bisporus (atcc 36416). the compounds were purified as their ferric chelates to homogeneity by gel permeation, cation exchange, and low-pressure reversed phase c18 chromatographies, and characterized as trihydroxamic acids. the chelates were identified as ferrichrome, ferric fusa ... | 1992 | 1447567 |
| bacterial mutagenicity of extracts of the baked and raw agaricus bisporus mushroom. | aqueous extracts of baked and raw agaricus bisporus (ab) mushroom were tested for mutagenic activity in salmonella typhimurium strains ta1535 and ta1537. the extracts were studied with and without metabolic activation by aroclor-induced rat liver s9 mix. the extracts of the baked and raw ab exhibited a dose related mutagenic activity with and without activation in strain ta1535. similar findings were obtained in strain ta1537, although the net revertant values were of lower magnitude. because hu ... | 1992 | 1457740 |
| sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase genes from the basidiomycetes schizophyllum commune, phanerochaete chrysosporium and agaricus bisporus. | gpd genes encoding glyceraldehyde-3-phosphate dehydrogenase were isolated from the homobasidiomycetes schizophyllum commune, phanerochaete chrysosporium and agaricus bisporus. all three species contain one transcriptionally active gpd gene, but a. bisporus also contains an inactive gpd gene (tandemly linked to the active gene). these genes contain 5-9 introns located at conserved positions, differing (except in one case) from intron positions in ascomycetous gpd genes. the predicted amino-acid s ... | 1992 | 1473176 |
| differential reactivity of agaricus bisporus lectin with human iga subclasses in gel precipitation. | the interaction between purified agaricus bisporus lectin and several human proteins was studied using the ouchterlony double diffusion and immunoelectrophoresis techniques. only one precipitation line was observed with normal human serum, normal human colostrum, iga1 myeloma serum, both serum monoclonal and secretory iga1 and monoclonal igd. no reaction was observed with monoclonal and secretory iga2, igg, igm, alpha 2 macroglobulin or pregnancy-associated alpha 2 glycoprotein. these results we ... | 1992 | 1474257 |
| the use of immobilized lectins in the separation of staphylococcus aureus, escherichia coli, listeria and salmonella spp. from pure cultures and foods. | lectins from helix pomatia, canavalia ensiformis, agaricus bisporus and triticum vulgaris agglutinated cultures of staphylococcus aureus, escherichia coli, listeria and salmonella spp. this agglutination was specific as it was inhibited (except with a. bisporus lectin) by the competing sugar substrates. the ability of three of these lectins, immobilized on a variety of supports, to separate these micro-organisms from pure cultures was investigated. immobilization of the lectins on magnetic micro ... | 1992 | 1512177 |
| growth-promoting effect of thermophilic fungi on the mycelium of the edible mushroom agaricus bisporus. | the growth-promoting effect of the thermophilic fungus scytalidium thermophilum in mushroom compost on the mycelium of the edible mushroom agaricus bisporus was investigated. results obtained by others were confirmed by showing that s. thermophilum leads to an increased hyphal extension rate of the mushroom mycelium. however, it was demonstrated that hyphal extension rates were not clearly related to mushroom biomass increase rates. a number of experiments pointed strongly towards co2 as the det ... | 1992 | 1514812 |
| agaritine does not mediate the mutagenicity of the edible mushroom agaricus bisporus. | ethanolic extracts of the edible mushroom agaricus bisporus displayed a direct-acting mutagenic response in various salmonella typhimurium strains, ta104 being clearly the most sensitive. incorporation of an activation system derived from the liver of mice, hamsters or aroclor 1254-induced rats failed to increase the mutagenic response. the mutagenic response of ethanolic extracts from various types of mushroom, containing different levels of agaritine (range 0.3-6.5 g/kg fresh weight), was very ... | 1991 | 1679191 |
| synapse formation occurs in association with the induction of long-term potentiation in two-year-old rat hippocampus in vitro. | following induction of long-term potentiation in subfield ca1 of the hippocampal slice from 26-month-old rats, shaft synapse numbers increased by 44% and sessile spine synapses (synapses on stubby, headless spines) by 72%, with the more common mushroom-shaped spine synapses statistically unaltered. these effects are smaller than in prior work with young adults, whereas population spike amplitude changes were comparable to young adults. reasons for the discrepancy are unclear, but the results con ... | 1991 | 1770987 |
| phenotypic variation of pseudomonas putida and p. tolaasii affects the chemotactic response to agaricus bisporus mycelial exudate. | the chemotactic response of wild-type pseudomonas putida and p. tolaasii, and a phenotypic variant of each species, to agaricus bisporus mycelial exudate was examined. both p. putida, the bacterium responsible for initiating basidiome development of a. bisporus, and p. tolaasii, the causal organism of bacterial blotch disease of the mushroom, displayed a positive chemotactic response to casamino acids and to a. bisporus mycelial exudate. the response was both dose- and time-dependent and marked ... | 1991 | 1791431 |
| phenotypic variation of pseudomonas putida and p. tolaasii affects attachment to agaricus bisporus mycelium. | the effect of phenotypic variation on attachment of pseudomonas tolaasii and p. putida to agaricus bisporus mycelium was investigated. quantitative studies demonstrated the ability of each isolate to attach rapidly and firmly to a. bisporus mycelium and significant differences in attachment of wild-type and phenotypic variant strains were observed. this was most pronounced in p. tolaasii, where the percentage attachment of the wild-type form was always greater than that of the phenotypic variant ... | 1991 | 1791432 |
| homology between mitochondrial dna of agaricus bisporus and an internal portion of a linear mitochondrial plasmid of agaricus bitorquis. | agaricus bisporus, the cultivated mushroom, contains a mitochondrial fragment (50h) which was previously demonstrated by southern hybridization to have sequence similarity to an internal region of pem, a linear mitochondrial plasmid of agaricus bitorquis. the nucleotide sequence of 50h was determined and compared to the sequence of the corresponding pem fragment. the region of sequence homology on pem is contained within an open reading frame (orf) that may encode an rna polymerase, but 50h is n ... | 1991 | 1879001 |
| sequences of three dsrnas associated with la france disease of the cultivated mushroom (agaricus bisporus). | la france disease of the cultivated mushroom, agaricus bisporus, is known to be associated with the presence of a number of dsrna segments. the nucleotide sequences of the dsrnas m2 (1.3 kb), m1 (1.55 kb) and l3 (2.8 kb), invariably associated with the disease, were determined. putative coding sequences for proteins with molecular weights of 38, 40 and 87 kda were found for m2, m1 and l3 dsrnas, respectively. the average g + c content of these dsrnas was 43%, close to that of a. bisporus nuclear ... | 1991 | 1934110 |
| left handed alpha-helix formation by a bacterial peptide. | the alpha-helix is a common element of secondary structure in proteins and peptides. in eukaryotic organisms, which exclusively incorporate l-amino acids into such molecules, stereochemical interactions make such alpha-helices, invariably right-handed. pseudomonas tolaasii paine is the causal organism of the economically significant brown blotch disease of the cultivated mushroom agaricus bisporus (lange) imbach. p. tolaasii proceduces an extracellular lipodepsipeptide toxin, tolaasin, which cau ... | 1991 | 1991518 |
| provocation tests in extrinsic allergic alveolitis in mushroom workers. | the clinical diagnosis of extrinsic allergic alveolitis can be supported by a positive provocation test. twenty-eight common mushroom (agaricus bisporus) workers, 4 oyster mushroom (pleurotus ostreatus) workers and 6 shii take mushroom (lentinus edodes) workers, whose medical history indicated a possible extrinsic allergic alveolitis, were examined. the provocation test consisted of a control day, an exposure day, and half a day of follow-up observation. on the control and exposure days, the bod ... | 1991 | 2030812 |
| carcinogenicity examination of agaricus bisporus, edible mushroom, in rats. | carcinogenicity of agaricus bisporus, the edible mushroom, was studied in rats. female charles river sprague - dawley rats (cd rats) were given a diet containing a 30% dry powder of a. bisporus for 500 days until the termination of the experiment. a control group was given a basal diet without a. bisporus. the rats in the experimental group had mammary tumor, thymoma, adrenal adenoma and pituitary adenoma. however, there was no significant difference in the incidence of these tumors between the ... | 1991 | 2049788 |
| characterization of galactose-containing glycoconjugates in the human retina: a lectin histochemical study. | seven specimens of morphologically normal formalin-fixed and paraffin-embedded human retina were studied using a panel of fourteen biotinylated lectins, all of which react with glycoconjugates containing galactose and n-acetylgalactosamine residues. agaricus bisporus (aba), bauhinia purpurea (bpa), phaseolus vulgaris (pha-e), peanut (pna) and ricinus communis (rca-i) agglutinins labeled photoreceptor cells prior to enzymatic predigestion. bpa and pna bound specifically to cones. the plexiform la ... | 1990 | 2091899 |
| benzoic acid inhibition of the alpha, beta, and gamma isozymes of agaricus bisporus tyrosinase. | inhibition of the catecholase and cresolase reactions of the alpha, beta, and gamma isozymes of agaricus bisporus tyrosinase by benzoic acid was investigated at 25.0 and 8.0 degrees c at ph 5.60 in air-saturated solutions. benzoic acid is a simple competitive inhibitor of the cresolase reaction of all three isozymes. in the catecholase reaction, however, benzoic acid is a partial uncompetitive inhibitor of the alpha and beta isozymes and a simple competitive inhibitor of gamma-tyrosinase. equili ... | 1990 | 2112900 |
| purification and characterization of an extracellular prolyl endopeptidase from agaricus bisporus. | prolyl endopeptidase [ec 3.4.21.26] was purified to homogeneity from the culture filtrate of agaricus bisporus by a procedure that comprised ammonium sulfate fractionation, anion-exchange chromatographies on deae-toyopearl and deae-sephadex, hydroxylapatite chromatography, and high-performance liquid chromatography (hplc) on a tskgel g 2000 sw column. the overall activity recovery was 8.6%. the enzyme was most active at or around ph 7.5 and was stable in the range of ph 5-9 when checked with z-g ... | 1990 | 2113924 |
| [quantities of agaritine in mushrooms (agaricus bisporus) and the carcinogenicity of mushroom methanol extracts on the mouse bladder epithelium]. | consumption of the agaricus species mushrooms has increased considerably in japan as the japanese have become accustomed to western cooking. the agaricus species mushroom contains hydrazine derivatives known as agaritine. bladder implantation was performed to test the carcinogenic potential of the agaricus species mushroom which contains large quantities of agaritine. the results are summarized as follows: 1) agaritine was detected in fresh agaricus bisporus mushrooms at the level of 228.2 micro ... | 1990 | 2132000 |
| agaricus bisporus metapotyrosinase: preparation, characterization, and conversion to mixed-metal derivatives of the binuclear site. | the alpha, beta, and gamma isozymes of agaricus bisporus tyrosinase undergo inactivation in the presence of oxalate. the inactivation rate law is first order in enzyme and second order in oxalate. on a more rapid time scale than inactivation, oxalate acts as a competitive inhibitor of the catecholase reaction of tyrosinase. after removal of oxalate by dialysis, the inactivated enzyme is found to contain 50% of the original copper, all of which is present as paramagnetic, mononuclear copper sites ... | 1990 | 2176854 |
| mutagenic and genotoxic activities of extracts derived from the cooked and raw edible mushroom agaricus bisporus. | a. bisporus has been reported to be carcinogenic to mice [toth et al. (1986) cancer res 38:177-180] and mutagenic in salmonella typhimurium [sterner et al. (1982) mutat res 101:269-281]. the effects of different heat treatments on the mutagenicity of raw, cooked (boiled) and fried a. bisporus extracts in the s. typhimurium test is reported. the spectrum of potential mutagenic activity of a. bisporus extracts was tested in vitro in syrian hamster embryo cells for selective dna amplification and i ... | 1990 | 2229137 |
| a study of the mutagenicity of some commercially canned spanish mushrooms. | the mutagenicity of two wild (lactarius deliciosus and boletus luteus) and two cultivated (agaricus bisporus and pleurotus ostreatus) mushrooms, preserved by canning and widely consumed in spain, was studied in the ames salmonella/microsome test and in the cho/hprt assay system using mammalian cells. the mushroom extracts did not show mutagenicity in the microbial ames test nor in the mammalian cho-k1 cells, and this response was not modified by the presence of s-9 mix in the assay mixtures. onl ... | 1990 | 2272557 |
| amino acids and peptides. xxvi. synthesis of agaricus bisporus metallothionein and related peptides and examination of their heavy metal-binding properties. | a pentacosapeptide corresponding to the entire amino acid sequence of agaricus bisporus metallothionein (mt) and related cysteine-containing peptides were prepared by the conventional solution method and their heavy metal-binding properties were examined. the cu2(+)- or cu(+)-binding activities of various peptides were not greatly dependent on the peptide structure, so far as examined, although the pentacosapeptide, a. bisporus mt, exhibited slightly higher binding activity than the other peptid ... | 1990 | 2279278 |
| stimulation of vascular cell proliferation by beta-galactoside specific lectins. | an investigation was conducted to assess the effects of various beta-galactoside specific lectins on the growth of vascular cells in vitro. the plant lectins from peanut (arachis hypogaea), mushroom (agaricus bisporus), and coral tree (erythrina corallodendron) were used in these studies with the ultimate purpose of comparing those findings with data derived with the lectin isolated from rat lung. peanut lectin was added to confluent and subconfluent cultures of smooth muscle cells (smc), pulmon ... | 1990 | 2379767 |
| a monoclonal antibody recognizing the fad-binding site of 4-aminobenzoate hydroxylase from agaricus bisporus. | a monoclonal antibody against 4-aminobenzoate hydroxylase (ec 1.14.13.27) from agaricus bisporus, a common edible mushroom, has been produced by the fusion of balb/c mouse spleen cells immunized with the denatured enzyme and p3x63ag8u1 myeloma cells in order to locate and characterize the catalytic site of the enzyme. the monoclonal antibody immunoblotted the enzyme and immunoprecipitated its apoenzyme. the immunoprecipitation was inhibited in the presence of fad, and the monoclonal antibody com ... | 1990 | 2398045 |
| natural pesticides and bioactive components in foods. | in this review, some common food plants and their toxic or otherwise bioactive components and mycotoxin contaminants have been considered. crucifers contain naturally occurring components that are goitrogenic, resulting from the combined action of allyl isothiocyanate, goitrin, and thiocyanate. although crucifers may provide some protection from cancer when taken prior to a carcinogen, when taken after a carcinogen they act as promoters of carcinogenesis. the acid-condensed mixture of indole-3-c ... | 1990 | 2404325 |
| cancer induction in mice by 4-hydroxybenzenediazonium sulfate of the agaricus xanthodermus mushroom. | 4-hydroxybenzenediazonium sulfate (hbd) was administered to swiss mice by subcutaneous injection at weekly intervals of two or 36 times at 10 or 2 micrograms per gram body weight, respectively. the hbd given 36 times induced tumors of the subcutis in 22% of females and in 22% of males. the corresponding tumor incidences in the untreated controls were 2% in females and 8% in males. histopathologically, the neoplasms were classified as fibromas, fibrosarcomas, myxosarcomas and rhabdomyosarcomas. t ... | 1989 | 2519869 |
| tumor induction by 4-(methyl)benzenediazonium sulfate in mice. | 4-(methyl)benzenediazonium sulfate (mbd) was administered to swiss mice by subcutaneous injection at weekly intervals 19 or 16 times at 50 micrograms/g body weight. the treatment gave rise to tumors of the subcutaneous tissue and skin. the tumor incidences in the groups with 19 injections were 12 and 6% in females and 12 and 4% in males, respectively, while in the group with 16 injections were 24 and 14% in females and 50 and 4% in males, respectively. the corresponding tumor incidences in the u ... | 1989 | 2519976 |
| inheritance of restriction fragment length polymorphisms in agaricus brunnescens. | the cultivated mushroom, agaricus brunnescens, is secondarily homothallic; most basidia produce only two basidiospores, each of which receives two of the four post meiotic nuclei. the segregation of restriction fragment length polymorphisms (rflps) detected by four plasmid probes carrying single-copy nuclear dna of agaricus was followed in seven parental strains including commercial, wild-collected, and artificially synthesized heterokaryons. of a total of 367 single-spore progeny examined, 351 ... | 1989 | 2573557 |
| effect of age on the binding of lectin 125i-pha-b to pancreatic islets of rat in vitro and stimulation of some cellular events. | agaricus bisporus lectin (pha-b) stimulates insulin release in vitro. the stimulation is associated with increased conversion of proinsulin to insulin in the isolated islets of langerhans of rats. both these functions are directly proportional to the binding of i125 pha-b, which is more marked in the islets from younger rats. the lectin binding to islets is not affected by glucose concentration in the medium. | 1989 | 2675521 |
| glycaemic effects of traditional european plant treatments for diabetes. studies in normal and streptozotocin diabetic mice. | twelve plants used for the traditional treatment of diabetes mellitus in northern europe were studied using normal and streptozotocin diabetic mice to evaluate effects on glucose homeostasis. the plants were administered in the diet (6.25% by weight) and/or as decoctions or infusions in place of drinking water, to coincide with the traditional method of preparation. treatment for 28 days with preparations of burdock (arctium lappa), cashew (anacardium occidentale), dandelion (taraxacum officinal ... | 1989 | 2743711 |
| inversion of 5s ribosomal rna genes within the genus coprinus. | in the basidiomycete fungus coprinus cinereus, the 5s ribosomal rna (rrna) genes are found in the rdna repeat which encodes the other three rrnas (18s, 5.8s, and 26s), and all genes are transcribed in the same direction (cassidy et al. 1984). to facilitate examination of the rrna gene organization in additional species, we developed a method to permit determination of the direction of transcription of the rrna genes using crude preparations of genomic dna. we used this method to determine the or ... | 1987 | 2835172 |
| the mutagenic activity of agaritine--a constituent of the cultivated mushroom agaricus bisporus--and its derivatives detected with the salmonella/mammalian microsome assay (ames test). | purified agaritine (n'-(gamma-l(+)-glutamyl)-p-hydroxymethylphenylhydrazine) isolated from agaricus bisporus, p-hydrazinobenzoic acid (its presumptive precursor) and some agaritine-degradation products were tested for mutagenic activity with the salmonella/mammalian microsome assay (ames test). consistent with the literature, agaritine showed a distinct direct-acting mutagenicity with the strain ta1537 (30 revertants/mumol) and with ta97. incubation of agaritine at alkaline ph increased the muta ... | 1986 | 2876563 |
| restriction fragment length polymorphisms in the mushrooms agaricus brunnescens and agaricus bitorquis. | two agaricus species, a. brunnescens (a commercial mushroom) and a. bitorquis (a wild, edible species), were examined for restriction fragment length polymorphisms. ecori-digested nuclear dna from isolates of both species were cloned in plasmid vector puc18. ten random recombinant clones were used in southern dna-dna hybridizations to probe ecori-digested dna from 11 a. brunnescens isolates (7 commercial, 2 wild type, and 2 homokaryotic) and 7 a. bitorquis isolates. most cloned fragments were po ... | 1987 | 2907275 |
| luminescence emission from the cu(i)-thiolate complex in metallothioneins. | the luminescence emission properties of cu-metallothioneins (from neurospora crassa, agaricus bisporus and livers of bedlington terriers affected by copper toxicosis) as well as of (cu,zn)-metallothionein from bovine fetal liver are reported. upon excitation in the u.v., these proteins emit a largely red-shifted luminescence with a maximum at 565 nm attributable to the cu(i)-thiolate chromophores of the proteins. differences in the shapes of the spectra and the emission intensity are observed wi ... | 1987 | 2959510 |
| cancer induction by the sulfate form of 4-(hydroxymethyl) benzenediazonium ion of agaricus bisporus. | the sulfate form of 4-(hydroxymethyl) benzenediazonium ion (hmbd) of agaricus bisporus was given by 26 weekly subcutaneous injections at 50 micrograms/g body weight to swiss mice. as a result of treatment, tumors of subcutis and skin developed in 32% and 14% of the females and 40% and 4% of the males, respectively. the corresponding tumor incidences in the sodium sulfate-injected solvent control groups were 0% and 2% in females and 4% and 0% in males, respectively. histopathologically, the tumor ... | 1987 | 2979762 |
| studies on prolyl endopeptidase from shakashimeji (lyophyllum cinerascens): purification and enzymatic properties. | high prolyl endopeptidase (post-proline cleaving enzyme) [ec 3.4.21.26] activity was detected in fruit bodies of shakashimeji (lyophyllum cinerascens), tsukuritake (mushroom: agaricus bisporus), hirohachichitake (lactarius hygrophoroides), and yaburebenitake (russula lepida) which belong to the genus basidiomycetes. cell-free extract of shakashimeji showed high activities of proline iminopeptidase and arylamidase as well as prolyl endopeptidase. the prolyl endopeptidase was purified from the ext ... | 1988 | 3071534 |
| comparative study of tyrosinases from different sources: relationship between halide inhibition and the enzyme active site. | the inhibition of tyrosinases from frog epidermis (rana esculenta ridibunda), mushroom (agaricus bisporus) and harding-passey mouse melanoma by halides is compared. in all cases, the inhibition is ph dependent, increasing when the ph decreases. the order of inhibition is i- greater than br- greater than cl- much greater than f- for frog epidermis tyrosinase, f- greater than i- greater than cl- greater than br- for mushroom tyrosinase and f- greater than cl- much greater than br- greater than i- ... | 1986 | 3082587 |
| antigenicity, catalytic activity and conformation of agaricus bisporus tyrosinase: interaction of conformation-directed antibodies with the native and irradiated enzyme. | the antiferromagnetically spin-coupled cu2+ pair present in the active center of tyrosinase was found to be indispensable for its catalytic function. however, the metal ion did not contribute to the conformational integrity or antigenicity of the enzyme molecule. irradiation of tyrosinase with 254 nm light resulted in dose dependent, essentially irreversible losses of its catalytic and antigenic functions. the apparent first order rate constants for the two processes were 17.6 x 10(-2) min-1 and ... | 1986 | 3084463 |
| active site and conformation-directed photoinactivation of copper-dependent oxidoreductases: studies of pig kidney diamine oxidase and agaricus bisporus tyrosinase. | | 1987 | 3129555 |
| [cultivation of pleurotus ostreatus and allied species (fungi: pleurotaceae) upon semi-sterile natural media]. | pleurotus ostreatus (jacq. ex fr.) quélet and its allies are edible mushrooms whose cultivation is successful in parts of europe and asia. in costa rica only agaricus bisporus has been commercially cultured, but requires elaborate facilities. the use of waste material (e.g. sawdust, rice straw, sugar cane debris) and non-controlled environmental conditions suitable for easy artisanal cultivation of pleurotus ostreatus is reported here. | 1988 | 3238078 |
| the structure of the mycelial wall of agaricus bisporus. | the chemical composition of fractions extracted from the mycelial walls of agaricus bisporus was studied. attempts were made to locate these materials "in situ" by comparing electron micrographs of shadowed and sectioned cell walls. there are at least two chemically distinct species of carbohydrate polymers: one yielding mainly glucose with lower amounts of mannose, galactose and xylose and amino acids, and the other yielding chiefly n-acetylglucosamine with some glucose and amino acids. it was ... | 1987 | 3269795 |
| a convenient procedure for rapid release of protoplasts from ustilago maydis. | filtrates from cultures of trichoderma harzianum, grown on a liquid mineral salts medium containing vitamin and trace element supplements, 0.5 g/l glucose and 2 g/l of dried residue of ethanol-extracted commercial mushrooms (agaricus brunnescens), are a good source of enzymes for the release of protoplasts from the sporidia of ustilago maydis. when concentrated 50-fold by (nh4)2so4 precipitation, the enzymes liberated 95% of the protoplasts from the sporidia within 40 min. | 1988 | 3273191 |
| carbohydrate-binding specificities of five lectins that bind to o-glycosyl-linked carbohydrate chains. quantitative analysis by frontal-affinity chromatography. | the carbohydrate-binding specificities of lectins purified from agaricus bisporus (aba-i), arachis hypogaea (pna), bauhinia purpurea (bpa), glycine max (sba), and vicia villosa (vva-b4) have been studied by affinity chromatography on columns of the immobilized lectins, and quantitatively analyzed by frontal affinity chromatography. these five lectins could be classified into two groups with respect to their reactivities with typical mucin-type glycopeptides, beta-d-galp-(1----3)-alpha-d-galpnac- ... | 1988 | 3274131 |
| purification and properties of 4-aminobenzoate hydroxylase, a new monooxygenase from agaricus bisporus. | a new fad-dependent monooxygenase, 4-aminobenzoate hydroxylase that catalyzes the decarboxylative hydroxylation of 4-aminobenzoate and forms 4-hydroxyaniline in the presence of nad(p)h and o2 has been purified to homogeneity by ammonium sulfate fractionation, affinity chromatography, chromatofocusing, and sephadex g-100 chromatography from agaricus bisporus, a common edible mushroom. the molecular weight of the enzyme, which consists of a single polypeptide, is 49,000. the enzyme contains 0.91 m ... | 1986 | 3489713 |
| aortic rupture and aortic smooth muscle tumors in mice. induction by p-hydrazinobenzoic acid hydrochloride of the cultivated mushroom agaricus bisporus. | p-hydrazinobenzoic acid (hba), an ingredient of the cultivated mushroom agaricus bisporus, was given in hydrochloride form at a dosage of 0.125% in drinking water for life to randomly bred swiss mice. previous studies had demonstrated that either synthetic or naturally occurring hydrazines are carcinogenic in mice with the main tumors so-induced being peripheral angiomas and angiosarcomas. as a result of hba treatment in the present experiments, smooth muscle cell tumors of the aorta and large a ... | 1987 | 3599914 |
| cancer induction in mice by feeding of the uncooked cultivated mushroom of commerce agaricus bisporus. | the cultivated mushroom of commerce in the western hemisphere, agaricus bisporus, was given p.o. to randomly bred swiss mice for 3 days and was followed by semisynthetic diet for 4 days each week for life. the mice were 6 weeks old at the beginning of the experiment. as a result of treatment, tumors were induced in the bone, forestomach, liver, and lungs in the following incidences: 16, 38, 8, and 40% in females and 16, 28, 12, and 62% in males, respectively. the corresponding tumor incidences i ... | 1986 | 3731070 |
| carcinogenesis by n2-[gamma-l(+)-glutamyl]-4-carboxyphenylhydrazine of agaricus bisporus in mice. | n2-[gamma-l(+)-glutamyl]-4-carboxyphenylhydrazine (gcph), an ingredient of the cultivated mushroom of commerce agaricus bisporus (ab), was administered as 52 weekly intragastric instillations of 1.4 mg/g body weight to randomly bred swiss mice. in comparison with the untreated controls, the subcutaneous tumor incidences rose from 0 to 26% in the males, while in the females the corresponding tumor incidences were 6 and 2%, respectively. histopathologically the tumors were fibrosarcomas, fibromas ... | 1986 | 3800334 |
| incorporation of chorismic acid and 4-aminobenzoic acid into the 4-hydroxyaniline moiety of n-(gamma-l-glutamyl)-4-hydroxyaniline in agaricus bisporus. | agaricus bisporus contains the unique aniline derivative, n-(gamma-l-glutamyl)-4-hydroxyaniline. 14c-labelled chorismic acid was quantitatively incorporated into the 4-hydroxyaniline moiety of this aniline derivative, whereas 14c-labelled prephenic acid and anthranilic acid were not incorporated into 4-hydroxyaniline. these observations indicate the branch point of the biosynthetic route of 4-hydroxyaniline in the shikimic acid pathway to be chorismic acid. moreover, 4-aminobenzoic acid proved t ... | 1985 | 3873258 |
| a unique enzyme catalyzing the formation of 4-hydroxyaniline from 4-amino-benzoic acid in agaricus bisporus. | a unique enzyme that catalyzes the formation of 4-hydroxyaniline from 4-aminobenzoic acid was found in the homogenate of agaricus bisporus. the enzyme was prepared from the homogenate by (nh4)2so4 fractionation, gel filtration and ion-exchange chromatography. the products formed from 4-aminobenzoic acid by the enzyme were shown to be 4-hydroxyaniline and co2. the reaction required fad, nad(p)h and o2. these results indicate that the enzyme is a new fad-dependent monooxygenase. | 1985 | 3875343 |
| jackfruit (artocarpus integrifolia) and the agaricus mushroom lectin fit also to the so-called peanut receptor. | the lectin from jackfruit (artocarpus integrifolia) reacts with the peanut-specific lectin receptor represented by the t-disaccharide, which is also part of the thomsen (= t)-friedenreich antigen, namely o-beta-d-galactosyl-(1----3)-n-acetyl-d-galactosamine. the difference between the jackfruit lectin and peanut lectin has been shown to be mainly due to the further linkage of the t-disaccharide: whereas the peanut lectin reacts with both anomeric forms, jackfruit lectin and the closely - with re ... | 1985 | 3938662 |
| purification and characterization of four isolectins of mushroom (agaricus bisporus). | four lectins were purified from a mushroom (agaricus bisporus) by ammonium sulfate fractionation, anion-exchange chromatography, affinity chromatography on bovine submaxillary mucin-sepharose 4b and preparative isoelectric focusing. they were designated as aba-i (pi 6.70), ii (pi 5.98), iii (pi 5.69) and iv (pi 5.53). polyacrylamide gel electrophoresis of each lectin in the presence of sodium dodecyl sulfate gave a single band with an apparent molecular mass of 16 000 da. sedimentation equilibri ... | 1985 | 4039942 |
| intracellular appearance of mushroom virus in fruiting bodies and basidiospores of agaricus bisporus. | | 1972 | 4110129 |