| a cytolytic protein from the edible mushroom, pleurotus ostreatus. | aqueous extracts of the edible mushroom, pleurotus ostreatus, contain a substance that is lytic in vitro for mammalian erythrocytes. the hemolytic agent, pleurotolysin, was purified to homogeneity and found to be a protein lacking seven of the amino acids commonly found in proteins. in the presence of sodium dodecyl sulfate it exists a monomers of molecular weight 12 050 whereas under non-dissociating conditions it appears to exist as dimers. it is isoelectric at about ph 6.4. the sensitivity of ... | 1979 | 573629 |
| glucose-2-oxidase activity and accumulation of d-arabino-2-hexosulose in cultures of the basidiomycete oudemansiella mucida. | submerged cultures of the basidiomycete oudemansiella mucida, strain iii, accumulate d-arabino-2-hexosulose. the maximum yields during cultivations in shaker flasks or in a laboratory fermentor are 6--12 and 15 mg/ml, respectively (20--50% conversion of substrate glucose). the accumulation is transient, the aldoketose being again utilized after glucose exhaustion. its production is stimulated by fluoride ions. the enzyme responsible for the c(2)-specific oxidation of d-glucose acts as an intrace ... | 1978 | 689573 |
| studies on mushroom flavours. 3. some flavour compounds in fresh, canned and dried edible mushrooms. | the concentrations of 1-octen-3-ol, 5'-gmp and glutamic acid were compared in 8 fresh, 3 canned and 5 dried mushroom species. the highest amounts of 1-octen-3-ol and 5'-gmp were found in the fresh muschrooms. agaricus bitorquis, pleurotus ostreatus and pholiota squarrosa contained 5-7 times as much 1-octen-3-ol as agaricus bisporus and calvatia gigantea 58 times as much. coprinus comatus and pleurotus ostreatus contained much 5'-gmp. little 1-octen-3-ol and 5'-gmp were found in most dried and ca ... | 1976 | 987665 |
| studies on the decomposition of lignosulfonates by the fungi pleurotus ostreatus and trametes pubescens. | the fungi pleurotus ostreatus and trametes pubescens were grown in a mineral medium containing 1% of glucose and 0.9% of lignosulfonates introduced into the culture medium in the form of yeast waste liquor. chromatography of extracts of the medium and determinations of sulphur and lignosulfonates have revealed that the fungi studied utilized the constituents of the yeast waste liquor (lignosulfonates) as carbon source. this was manifested in an increase of dry mass of the mycelium and protein as ... | 1975 | 1172650 |
| recovery of recombinant plasmids from pleurotus ostreatus transformants. | a transformation system employing selectable resistance to hygromycin b has been developed for the mushroom-forming fungus, pleurotus ostreatus. vector pan7-1, a commonly used non-replicative vector for integrative transformation in fungi, yielded 5-46 resistant colonies per micrograms of dna per 10(7) viable protoplasts. southern blot analysis of certain transformants revealed unexpected replicative plasmids containing pan7-1 sequences, but modified for size, methylation and restriction enzyme ... | 1992 | 1611668 |
| basidiospore extracts: evidence for common antigenic/allergenic determinants. | spore extracts, prepared from armillariella tabescens, pleurotus ostreatus, coprinus quadrifidus, amanita muscaria, ganoderma lucidum, psilocybe cubensis, pisolithus tinctorius, scleroderma sp. and calvatia cyathiformis, were examined for antigenic/allergenic relationships by ouchterlony and radioallergosorbent testing (rast) inhibition, respectively. ouchterlony, using hyperimmunized rabbit sera, demonstrated a high degree of cross-antigenicity among the extracts tested; however, some unique an ... | 1988 | 2448249 |
| [levels of volatile fatty acids and cellulase activity in rumen fluid after feeding the substrate remaining after the cultivation of oyster mushrooms (pleurotus ostreatus)]. | | 1988 | 3135652 |
| toxicologic and histopathologic studies of pleurotus ostreatus mushroom in mice. | the edible mushroom pleurotus ostreatus (with locally reported toxic properties) was identified and collected 1-4 days after raining in the city of baghdad. it was freshly extracted with distilled water at room temperature. both oral and intraperitoneal routes were used for acute and subacute toxicity studies on mice. in spite of important toxicologic signs and findings on treated animals, the estimated 24-h ld50 values exceeded 3 g/kg for both routes of administration. however, the 30-day ld50 ... | 1987 | 3441138 |
| characterization of lignin peroxidase-encoding genes from lignin-degrading basidiomycetes. | two closely linked lignin peroxidase (lpo)-encoding genes (lpo) from phanerochaete chrysosporium were isolated. nucleotide sequence studies indicated that the two genes are separated by 1.3 kb of flanking dna and transcribed in opposite directions. cloned p. chrysosporium lpo gene probes have been shown to hybridize to multiple sequences present in the dnas of the white-rot fungi, bjerkandera adusta, coriolus versicolor and fomes lignosus, but no hybridization was detected with dna from pleurotu ... | 1990 | 2373364 |
| [respiratory tract diseases in oyster mushroom cultivators]. | oyster mushrooms are cultivated for human consumption in various subspecies. their spores are a highly potent allergen, causing an exogenous allergic alveolitis. relevant early pointers are the typical anamnesis, reduced vital capacity without any signs of obstruction, slight changes on x-ray film, positive immunofluorescence test. frequent and typical wrong diagnoses during the initial episodes of the disease are: respiratory infection, influenza, pleuritis, type i allergy. so far, antibodies w ... | 1990 | 2367412 |
| a study of the mutagenicity of some commercially canned spanish mushrooms. | the mutagenicity of two wild (lactarius deliciosus and boletus luteus) and two cultivated (agaricus bisporus and pleurotus ostreatus) mushrooms, preserved by canning and widely consumed in spain, was studied in the ames salmonella/microsome test and in the cho/hprt assay system using mammalian cells. the mushroom extracts did not show mutagenicity in the microbial ames test nor in the mammalian cho-k1 cells, and this response was not modified by the presence of s-9 mix in the assay mixtures. onl ... | 1990 | 2272557 |
| evaluation of basidiomycete and deuteromycete (fungi imperfecti) extracts for shared allergenic determinants. | aqueous extracts of select members of the basidiomycetes and deuteromycetes (fungi imperfecti) were evaluated for the presence of shared allergenic determinants using skin prick and radio-allergosorbent test (rast) inhibition. twenty adults with perennial symptoms of rhinitis, with or without asthma, were skin-prick tested with six species of deuteromycetes and seven species of basidomycetes. positive weal-and-flare reactivity to pleurotus ostreatus was associated with alternaria alternata, fusa ... | 1990 | 2253084 |
| response of pleurotus ostreatus to cadmium exposure. | the possibility of utilizing agroindustrial wastes in the production of edible, high-quality products (e.g., mushrooms) implies the risk of bringing toxic substances, such as heavy metals, into the human food chain. thus, growth in the presence of cadmium and cadmium accumulation limits have been studied in the industrially cultivated fungus p. ostreatus. fruit body production is substantially unaffected in the presence of 25, 139, and 285 mg cd/kg of dried substrate. cadmium concentration in fr ... | 1990 | 2226238 |
| evidence for cross-reactive allergens among basidiomycetes: immunoprint-inhibition studies. | allergenic cross-reactivity among six basidiomycete species (calvatia cyathiformis, coprinus quadrifidus, psilocybe cubensis, pleurotus ostreatus, ganoderma meredithae, and pisolithus tinctorius) was determined by immunoprint inhibition. extensive cross-reactivity was demonstrated among coprinus quadrifidus, psilocybe cubensis, and pleurotus ostreatus of the order agaricales, and calvatia cyathiformis of the order lycoperdales. however, g. meredithae (order aphyllophorales) and pisolithus tincto ... | 1990 | 2196301 |
| effects of oyster mushroom pleurotus pulmonarius on preparasitic larvae of bovine trichostrongyles. | | 1990 | 2099631 |
| provocation tests in extrinsic allergic alveolitis in mushroom workers. | the clinical diagnosis of extrinsic allergic alveolitis can be supported by a positive provocation test. twenty-eight common mushroom (agaricus bisporus) workers, 4 oyster mushroom (pleurotus ostreatus) workers and 6 shii take mushroom (lentinus edodes) workers, whose medical history indicated a possible extrinsic allergic alveolitis, were examined. the provocation test consisted of a control day, an exposure day, and half a day of follow-up observation. on the control and exposure days, the bod ... | 1991 | 2030812 |
| concentrations of radiocesium and potassium in basidiomycetes collected in japan. | concentrations of 137cs, 134cs and 40k in about 60 mushroom samples (fruit bodies of basidiomycetes) belonging to 25 species collected in japan have been studied. the levels of 137cs varied very widely, ranging from less than 3 to 1520 bq kg-1 (dry wt), while those of 40k were relatively constant. concentrations of 137cs in common edible mushrooms of japan such as lentinus edodes, flammulina velutipes, pleurotus ostreatus and pholiota nameko were low (normally less than 50 bq kg-1, dry wt). conc ... | 1991 | 1925522 |
| cholesterol-lowering effect of the mushroom pleurotus ostreatus in hereditary hypercholesterolemic rats. | we studied the effect of the edible mushroom pleurotus ostreatus (4% in diet containing 1% of cholesterol) on serum and liver lipids in female rats with hereditary enhanced sensitivity to alimentary cholesterol. we found that the consumption of the mushroom-containing diet prevented serum cholesterol increase which was manifested at the end of the 4th week of the experiment. at the end of the 7th week of the experiment the cholesterolemia was lowered by almost 40% as compared with control animal ... | 1991 | 1897899 |
| effect of mushroom pleurotus ostreatus and isolated fungal polysaccharide on serum and liver lipids in syrian hamsters with hyperlipoproteinemia. | in syrian hamsters, a diet with 44% of the calories being fat and containing 52 mg cholesterol (c)/100 g induced an accumulation of blood plasma and liver c and triacylglycerol (tg). in these animals, we studied the effect of dried whole mushroom (pleurotus ostreatus, 2% in the diet, 6-mo experiment) and ethanol-insoluble residue and structurally defined fungal polysaccharide, both isolated from the mushroom (in both cases, 4% in the diet, 2-mo experiments) on c and tg concentration in serum and ... | 1991 | 1802191 |
| ability of the fungus pleurotus pulmonarius to immobilise preparasitic nematode larvae. | in vitro experiments showed that the oyster mushroom pleurotus pulmonarius exerts a strong immobilising effect on preinfective larvae of the animal parasitic nematodes ostertagia ostertagi, cooperia oncophora, oesophagostomum quadrispinulatum and cyathostoma species. infective stages of the same species seemed less vulnerable to fungal attack, possibly because they are protected by their outer cuticle. | 1991 | 1780575 |
| effect of oyster fungus (pleurotus ostreatus) on serum and liver lipids of syrian hamsters with a chronic alcohol intake. | the authors studied the effect of oyster fungus (pleurotus ostreatus) (2% dried fruiting bodies in a standard diet) on the serum and liver lipids of growing male syrian hamsters with a chronic alcohol intake (a 15% aqueous solution). after eight weeks' alcohol intake there was an increase in their serum cholesterol, triacylglycerol (tg) and phospholipid (pl) concentration, 40 - 60% of which was accounted for by an increase in the very low density lipoprotein (vldl) concentration. the proportion ... | 1991 | 1751479 |
| fungal biodegradation of lignopolystyrene graft copolymers. | white rot basidiomycetes were able to biodegrade styrene (1-phenylethene) graft copolymers of lignin containing different proportions of lignin and polystyrene [poly(1-phenylethylene)]. the biodegradation tests were run on lignin-styrene copolymerization products which contained 10.3, 32.2, and 50.4% (wt/wt) lignin. the polymer samples were incubated with the white rot fungi pleurotus ostreatus, phanerochaete chrysosporium, and trametes versicolor and the brown rot fungus gloeophyllum trabeum. w ... | 1992 | 1444360 |
| mushroom worker's lung. detection of antibodies against shii-take (lentinus edodes) spore antigens in shii-take workers. | indoor cultivation of the edible mushroom shii-take (lentinus edodes) regularly leads to symptoms of mushroom worker's lung (mwl) in workers. an immunologic test is described allowing detection of igg type antibodies against shii-take spore antigens. it was found that mwl patients employed in shii-take picking (n = 5) have significantly increased antibody titres against shii-take spore antigens. different control groups, viz, mwl patients employed in the cultivation of the white button mushroom ... | 1992 | 1432300 |
| the mushroom pleurotus ostreatus accelerates plasma very-low-density lipoprotein clearance in hypercholesterolemic rat. | the administration of a diet containing 5% of dried oyster mushroom to male wistar rats fed a cholesterol diet (0.3%) shortly after weaning for 8 weeks reduced cholesterol levels in the serum and liver by 27 and 33%, respectively and increased the fractional turnover rate of 125i-very-low-density lipoproteins (vldl) by more than 30%. | 1994 | 7993890 |
| [current aspects of fungal spores allergy]. | in industrialized countries the prevalence of allergic inhalant diseases is some 15-20%. more than 10% of these individuals are sensitized to fungal allergens. many fungal spores are less than 10 microns in size, which permits penetration into the smaller airways of the lung. bronchial provocation tests have demonstrated that fungal spores and spore extracts can cause both an early and a late phase reaction in sensitive subjects. over 80 genera of fungi have been associated with symptoms of resp ... | 1994 | 8016603 |
| [the effect of fungal and yeast glucan and levamisole on the level of the cellular immune response in vivo and leukocyte phagocytic activity in mice]. | the level of cell-mediated immune response in vivo was investigated using the test of delayed hypersensitive reaction (dhr) to dnfb, along with the phagocytic activity (pa) of blood leucocytes in mice after subcutaneous implantation of fungal and yeast glucan and levamisol in dependence on the dose and administration schedule. the soluble form of fungal glucan (pleurotus ostreatus) potentiated the dhr significantly at a dose of 10 mg/kg (but not at a dose of 50 mg/kg) while it was administered d ... | 1992 | 1297245 |
| [the effect of administration of non-soluble fungal glucan on the level of delayed skin hypersensitivity and phagocytic activity of leukocytes in the blood of calves]. | the level of delayed skin hypersensitivity (dsh) was evaluated in vivo after dinitrofluorobenzene induction (3rd and 7th week of the experimental) while the level of phagocytic activity (fa) of blood leucocytes (in weekly intervals during six weeks of observation) was evaluated in calves (4.5 to 5.5 months of age) after administration of particular fungal glucane (from oyster mushroom) at a dose of 10 mg/kg live weight. in calves which were administered glucane (abbr. g), a significantly stronge ... | 1992 | 1292166 |
| mechanism of hypocholesterolemic effect of oyster mushroom (pleurotus ostreatus) in rats: reduction of cholesterol absorption and increase of plasma cholesterol removal. | the content of cholesterol in the serum and liver of male wistar rats fed, for the period of 8 weeks shortly after weaning, a diet containing 0.3% of cholesterol was reduced by 33 and 27% by the addition of 5% of dried oyster mushroom powder. although the level of serum triacylglycerols was not affected by oyster mushroom, their content in liver of rats on mushroom diet was reduced by 41%. very-low-density lipoproteins and low-density lipoproteins participated by 55 and 38%, respectively, in the ... | 1994 | 8197787 |
| [mushroom worker's lung caused by inhalation of spores of the edible fungus pleurotus florida ("oyster mushroom") (author's transl)]. | a new edible mushroom, known as oyster mushroom (pleutrotus florida, variety of pleurotus ostreatus) has recently gained commercial importance in europe, having been imported from the u.s.a. in 1963 and cultivated in germany between 1972 and 1973. several persons concerned with the cultivation and industrial production of this mushroom fell ill, after close contact with it over a period of some months, with exhaustion, headache, chills and fever, and cough. these symptoms, similar to those of "f ... | 1976 | 820538 |
| induction of laccase in basidiomycetes: apparent activity of the inducible and constitutive forms of the enzyme with phenolic substrates. | the purified preparations of the inducible and constitutive forms of laccase (ec 1.14.18.1) have been obtained from mycelia of trametes versicolor, pleurotus ostreatus and pholiota mutabilis. the activities of the inducible forms of laccase with ferulic acid and other phenolic hydrogen donors were found to be several-fold higher as compared with the constitutive forms. | 1978 | 108885 |
| induction of laccase in basidiomycetes: the laccase-coding messenger. | formation of the mrna specific for the inducible forms of laccase was evidenced in coriolus versicolor, pleurotus ostreatus and pholiota mutabilis. the half-life time of these mrnas in the fungi species studied were, respectively, 30, 37 and 24 min. molecular weight of the newly synthesized mrna in pleurotus ostreatus was about 4.5x10(5), consistently with the size of the inducible laccase protein. the polysome obtained from the ferulic acid-treated mycelium, synthesized in vitro a polypeptide w ... | 1978 | 103348 |
| immunological study on the wall proteins of different fruiting portions in pleurotus ostreatus (jacq. ex fr.) kummer and agaricus bisporus (lge.) sing. | immunological techniques are useful to clarify the sistematic scheme utilized for mushrooms. by comparing the various fruiting portions, a different composition in protein was observed in the cell wall of spores, pileus and stipe. the differences were present both in agaricus bisporus and in pleurotus ostreatus. the results indicate that a well definite choice of the utilized portions is necessary for comparative studies. in the basidiomycetes the meiospores represent the more useful development ... | 1983 | 6442242 |
| the mushroom pleurotus ostreatus reduces secretion and accelerates the fractional turnover rate of very-low-density lipoproteins in the rat. | in male rats fed a diet containing 1.5% cholesterol and 5% of dried mushroom (pleurotus ostreatus) a significantly reduced accumulation of cholesterol in serum (by 45%) and the liver (by 15%) was observed at the end of the 12th week of the experiment. the decrease in serum cholesterol level by more than 90% is a consequence of the decreased cholesterol concentration of very-low-density lipoproteins (vldl) and of low-density lipoproteins. consumption of p. ostreatus reduces the total vldl entry i ... | 1993 | 8373138 |
| biodegradation of ddt [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] by the white rot fungus phanerochaete chrysosporium. | extensive biodegradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (ddt) by the white rot fungus phanerochaete chrysosporium was demonstrated by disappearance and mineralization of [14c]ddt in nutrient nitrogen-deficient cultures. mass balance studies demonstrated the formation of polar and water-soluble metabolites during degradation. hexane-extractable metabolites identified by gas chromatography-mass spectrometry included 1,1,-dichloro-2,2-bis(4-chlorophenyl)ethane (ddd), 2,2,2-trichlo ... | 1987 | 3674869 |
| basidiospore allergens: determination of optimal extraction methods. | five methods were tested by rast for allergen extraction from seven basidiospore species armillaria tabescens, chlorophyllum molybdites, coprinus quadrifidus, pleurotus ostreatus, calvatia cyathiformis, pisolithus tinctorius, and scleroderma sp. with each basidiospore type, extracted allergen activity varied according to the method employed. in general, defatting of spores with ethyl ether, followed by homogenization in 0.125 m nh4hco3 buffer, resulted in greatest allergen yield. in order to com ... | 1987 | 3608137 |
| first evidence for the occurrence of n delta-acetyl-l-ornithine and quantification of the free amino acids in the cultivated mushroom, pleurotus ostreatus. | the free amino acid profile of the fruiting bodies of pleurotus ostreatus was studied in detail. the first evidence for the occurrence of n delta-acetyl-l-ornithine in the mushroom was presented, and 33 ninhydrin-positive compounds were determined, besides n delta-acetyl-l-ornithine. alanine, glutamic acid, valine, glutamine, glycine and leucine were predominant protein amino acids occurring in the free form. as regards the non-protein amino acids, ornithine, gamma-aminobutyric acid, saccharopin ... | 1984 | 6539816 |
| [basidiomycetes in relation to antibiosis. ii. antibiotic activity of mycelia and culture liquids]. | the author relates the results of the antibiotic activity of mycelia and culture liquids of 34 basidiomycetes, tested against escherichia coli, staphylococcus aureus, bacillus subtilis and candida albicans. the mycelium disc test and the cylinder plate method were used, employing for the latter culture liquids at different ages. the most active against the three bacterial species were psathyra spadiceo-grisea, fistulina hepatica and fomes pinicola; against s. aureus and b. subtilis, lentinus edo ... | 1981 | 6813176 |
| bioaccumulation of hg in the mushroom pleurotus ostreatus. | the possibility of utilizing industrial, urban, and other wastes for the growth of a product which is directly edible by humans is fascinating. however, it is possible that many wastes containing toxic substances, for example, heavy metals, could reach the food chain and produce adverse effects on human health. to this end, we studied the possibility of bioaccumulation of hg by a mushroom, pleurotus ostreatus, grown on an artificial compost containing this element. concentrations of 0.05, 0.1, a ... | 1988 | 3234295 |
| [cultivation of pleurotus ostreatus and allied species (fungi: pleurotaceae) upon semi-sterile natural media]. | pleurotus ostreatus (jacq. ex fr.) quélet and its allies are edible mushrooms whose cultivation is successful in parts of europe and asia. in costa rica only agaricus bisporus has been commercially cultured, but requires elaborate facilities. the use of waste material (e.g. sawdust, rice straw, sugar cane debris) and non-controlled environmental conditions suitable for easy artisanal cultivation of pleurotus ostreatus is reported here. | 1988 | 3238078 |
| basidiomycete allergy: what is the best source of antigen? | skin prick test activity and antigenicity of extracts of in vitro growth of the basidiomycete pleurotus ostreatus (po) were compared to extracts of spores from po growing in the wild. patients demonstrated significant differences in skin test reactivity to the po extracts. some reacted only to in vitro growth extracts, others only to the spore extracts and 1 patient to all extracts. further studies analyzed antigens present in all extracts with rabbit antisera to po. common as well as unique ant ... | 1985 | 4008070 |
| dietary oyster mushroom (pleurotus ostreatus) accelerates plasma cholesterol turnover in hypercholesterolaemic rat. | the effect of adding 5% powdered oyster mushroom (pleurotus ostreatus) during 12 weeks on kinetic parameters of cholesterol metabolism was studied in male rats (wistar, initial body weight 85 g) fed a semisynthetic diet containing 0.3% of cholesterol. the plasma cholesterol decay curve (examined for the final 29 days of the experiment after a single dose of cholesterol-4-14c) was evaluated by mathematical analysis using a two-pool model of plasma cholesterol metabolism. the oyster mushroom in th ... | 1995 | 8869262 |
| the relationship between pleurotus ostreatus and aspergillus flavus and the production of aflatoxin. | the relationship between pleurotus ostreatus and aspergillus flavus in common mixed culture on various substrates was investiaged. it was found that p. ostreatus, similarly to some other higher fungi, can liquidate colonies of a. flavus. this fungus does not produce aflatoxin and chromatographically similar compounds. on straw, corn cobs, millet and wheat a. flavus produced aflatoxin after a 3-week cultivation. a subsequent cultivation of p. ostreatus led to detoxication of straw and corn cobs b ... | 1980 | 7419131 |
| new antifungal bithienylacetylenes from blumea obliqua. | two new bioactive bithienylacetylenes isolated from blumea obliqua have been characterized as 5'-methyl-5-[4-(3-methyl-1-oxobutoxy)-1-butynyl]-2,2'- bithiophene [1] and 5'-hydroxymethyl-5-[butyl-3-en-1-yn]-2,2'-bithiophene isovaleroxy ester [2], respectively. compounds 1 and 2 showed antifungal activity against epidermophyton floccosum and pleurotus ostreatus. | 1995 | 7494149 |
| [mineral and amino acid contents of edible, cultivated shii-take mushrooms (lentinus edodes)]. | world wide about 200,000 tons of shii-take mushrooms (lentinus edodes) are produced per year. different positive biological effects are known (anticarcinogenic, anticholesterol, immunostimulating effects), but the mineral contents and amino acid composition of caps and stipes are still little investigated. the concentrations of minerals are in general lower than those in the cultivated white mushroom (agaricus bisporus) and in the oyster mushroom (pleurotus ostreatus). the greatest differences a ... | 1995 | 7571860 |
| presence of a p1 bacteriophage sequence in transforming plasmids of pleurotus ostreatus. | replicative plasmids pp01 and pp02, recovered from pleurotus ostreatus transformants, contain an insert of bacteriophage origin. these plasmids have been amplified by the polymerase chain reaction (pcr) and have been shown to represent a low-grade component in the initial preparation of the vector pan7-1. the pp01 and pp02 plasmids share an insert (p01a) of virtual identity with a smai-bamhi genomic fragment of p 1 bacteriophage and retain remnants of a polylinker at the 5' end of this fragment. ... | 1995 | 7586033 |
| oyster mushroom (pleurotus ostreatus) reduces the activity of 3-hydroxy-3-methylglutaryl coa reductase in rat liver microsomes. | the effect of dried oyster mushroom (pleurotus ostreatus) on cholesterol (c) content in serum, in lipoproteins and in liver, and on the activity of 3-hydroxy-3-methylglutaryl coa (hmg-coa) reductase in liver microsomes, was studied in male rats (strain wistar, initial body weight 75 g) fed on low-cholesterol (9 mg/100 g) and high-cholesterol (0.3%) diets. addition of 5% oyster mushroom to both diets reduced significantly the c-content in serum (by 30%), in very-low- and low-density lipoproteins ... | 1995 | 7607302 |
| solid-state fermentation of agricultural wastes into food through pleurotus cultivation. | the technical feasibility of using agricultural wastes (mango and date industry wastes) as a substrate for the cultivation of pleurotus ostreatus nrrl-0366 is evaluated. when comparing the biological efficiency of mushroom production, the highest yield of fruiting bodies was obtained using a mixture of date waste and rice straw at a ratio (1:1) (11.96%), followed by a mixture 3:1 (11.16%). the lowest one was the mixture 2:1 (9.19%). fungus pleurotus ostreatus nrrl-0366 can also be cultivated on ... | 1995 | 7626144 |
| antioxidative effect of oyster mushroom (pleurotus ostreatus) in hypercholesterolemic rat. | | 1995 | 7651988 |
| enhancement of the resistance of tilapia and grass carp to experimental aeromonas hydrophila and edwardsiella tarda infections by several polysaccharides. | efficacies of eleven polysaccharides including bar (glycan extracted from barley), curdlan, dex (dextran sulfate), inulin, krestin, laminaran, levan, po (glycan extracted from pleurotus ostreatus), scleroglucan, yg (yeast glucan), and zymosan, in the protection of tilapia, tilapia aureus p., and grass carp, ctenopharyngodon idellus, against bacterial infections in vivo were examined. four glycans. namely, bar, krestin, scleroglucan, and zymosan were observed to significantly increase the surviva ... | 1997 | 9280393 |
| removal of pcbs by various white rot fungi in liquid cultures. | the ability of phanerochaete chrysosporium, trametes versicolor, coriolopsis polyzona, and pleurotus ostreatus growing in a mitogen-limited mineral medium (nmm) to degrade pcbs in a commercial, delor 106 mixture at a concentration of 0.9 ppm was compared. the respective amount of pcbs removed from the fungal cultures within 3 weeks were 25, 50, 41 and 0%. the capacities of the individual fungal species to remove pcbs correlated to some extent with their capabilities of decolorization of nmm agar ... | 1997 | 9340310 |
| [phytotherapeutic aspects of diseases of the circulatory system. 4. chitin and chitosan]. | chitin and chitosan are nitrogenous polysaccharides. the structure of the chitin molecule is similar to that of cellulose but it is composed of the units of 2-acetylamino-2-deoxy-d-glucopyranose bound (1 --> 4) by a glycosidic bond. in contrast to chitin, chitosan amino groups are not mostly acetylated. these polysaccharides occur particularly in the fungal kingdom, in invertebrate animals, in some brown algae, and very negligibly in higher plants. great attention has been paid to chitin and chi ... | 1995 | 7663872 |
| effect of the oyster fungus on glycaemia and cholesterolaemia in rats with insulin-dependent diabetes. | we have investigated the effect of a diet containing of 4% oyster fungus (pleurotus ostreatus) and 0.1% cholesterol on glycaemia and hyperlipoproteinaemia in rats with insulin-dependent diabetes (streptozotocin 45 mg/kg). after two months, the rats with diabetes kept on the oyster fungus diet, had a significantly lower basal and postprandial glycaemia, the insulinaemia remained unchanged. the cholesterol concentration was decreased by more than 40%, the lipoprotein profile was upgraded by the de ... | 1993 | 7710476 |
| the effect of oyster mushroom (pleurotus ostreatus), its ethanolic extract and extraction residues on cholesterol levels in serum, lipoproteins and liver of rat. | | 1995 | 7898579 |
| prevalence of basidiomycete allergy in the usa and europe and its relationship to allergic respiratory symptoms. | a total of 701 adults living in the usa or western europe who had symptoms of allergic respiratory disease were skin prick tested with extracts prepared from eight basidiomycetes species and four fungi imperfecti species. in these subjects, the presence of asthma, rhinitis, or both was determined by questionnaire. overall, 178/701 (25.4%) of the participants reacted to at least one basidiomycete extract. there was no difference in the prevalence of reactivity detected in the usa and europe (p < ... | 1994 | 8074266 |
| influence of water and ethanol extracts of the oyster mushroom (pleurotus ostreatus) on serum and liver lipids of the syrian hamsters. | extracts from the dried and ground fungus were prepared with water and with 30%, 60% and 85% ethanol, and thickened in vacuum. the whole fungus and extracts were added to the hyperlipidemic diet in amounts equivalent to 3% of the whole fungus. after 6 weeks the whole fungus, its water as well as 30% and 60% ethanol extracts have significantly reduced the contents of cholesterol (c) and triacylglycerols (tg) in the serum. the c and tg contents of the liver were reduced by 34-48% (in the case of t ... | 1993 | 8121469 |
| effect of the oyster fungus on glycaemia and cholesterolaemia in rats with insulin-dependent diabetes. | we have investigated the effect of a diet containing of 4% oyster fungus (pleurotus ostreatus) and 0.1% cholesterol on glycaemia and hyperlipoproteinaemia in rats with insulin-dependent diabetes (streptozotocin 45 mg/kg). after two months, the rats with diabetes kept on the oyster fungus diet, had a significantly lower basal and postprandial glycaemia, the insulinaemia remained unchanged. the cholesterol concentration was decreased by more than 40%, the lipoprotein profile was upgraded by the de ... | 1993 | 8218150 |
| [delayed hypersensitivity and the primary antibody reaction after administration of infectious bovine rhinotracheitis inactivated oil vaccine to calves premedicated with glucan]. | the level of delayed skin hypersensitivity (dsh) to dnfb and of the primary immune reaction was evaluated in the calves immunized with an inactivated oil ibr vaccine (v group) and in the calves premedicated with glucan (seven days before vaccine administration) and subsequently immunized with the mentioned vaccine (gv group). the dsh test did not reveal an alteration of cellular immunological reactivity in the calves immunized with an inactivated oil ibr vaccine; after the vaccine administration ... | 1993 | 8379100 |
| bending of dna segments with saccharomyces cerevisiae autonomously replicating sequence activity, isolated from basidiomycete mitochondrial linear plasmids. | previous studies have indicated that dna bending is a general structural feature of sequences (arss) from cellular dnas of yeasts and nuclear and mitochondrial genomic dnas of other eukaryotes that are capable of autonomous replication in saccharomyces cerevisiae. here we showed that bending activity is also tightly associated with s. cerevisiae ars function of segments cloned from mitochondrial linear dna plasmids of the basidiomycetes pleurotus ostreatus and lentinus edodes. two plasmids, desi ... | 1993 | 8455547 |
| purification and characterization of intracellular proteinases in pleurotus ostreatus fruiting bodies. | a serine proteinase (proa, ec 3.4.22.9) and two metalloendopeptidases (prob, ec 3.4.99.32 and proc, 3.4.24.4), have been purified to homogeneity from the fruiting bodies of pleurotus ostreatus. proa is a serine proteinase with a mass of 30 kda, which has amidolytic and esterolytic activities besides proteolysis and catalyzes preferential cleavage of the peptide bonds involving the carboxyl groups of hydrophobic amino acid residues in oxidized bovine insulin b chain. the n-terminal amino acid seq ... | 1995 | 8541645 |
| purification and catalytic properties of two manganese peroxidase isoenzymes from pleurotus eryngii. | the ligninolytic basidiomycetes pleurotus eryngii, pleurotus ostreatus, pleurotus pulmonarius and pleurotus sajor-caju did not exhibit detectable levels of manganese peroxidase (mp) when grown in liquid media with ammonium tartrate as n source. however, after examination of cells grown on different organic n-based media, high mp activity was obtained in peptone medium, up to nearly 3 u/ml in cultures of p. eryngii. moreover, mn2+ supplementation was not used to produce mp, since all mn2+ concent ... | 1996 | 8647081 |
| [chemical composition of three mexican strains of mushrooms (pleurotus ostratus)]. | the chemical composition of three mexican strains of pleurotus ostreatus (inireb-8, cdbb-h-896 and cdbb-h-897), were determined. the mushrooms were cultivated on wheat straw in a greenhouse (22-28 degrees c temperature and 80 +/- 5% of relative humidity). fruits bodies of p. ostreatus contained (all values are expressed in g/100 g dry wt.), protein (n x 6.25): 24.64 +/- 0.21-28.50 +/- 0.26; lipids: 1.10 +/- 0.16-1.85 +/- 0.22; mineral matter: 7.66 +/- 0.23-8.79 +/- 0.25; dietary fibre: 32.14 +/- ... | 1998 | 10347703 |
| mode of action and active site of an extracellular peroxidase from pleurotus ostreatus. | the properties of the haem environment of an extracellular peroxidase from pleurotus ostreatus were studied by electronic absorption spectroscopy. a high-spin ferric form was predominant in the native enzyme and a high-spin ferrous form in the reduced enzyme. cyanide was readily bound to the haem iron in the native form, thereby changing the enzyme to a low-spin cyano adduct. the electronic absorption spectra of the enzyme were similar to those of lignin peroxidase from phanerochaete chrysospori ... | 1996 | 8670051 |
| effect of cultivation conditions on cellulase activity of higher fungi. | production of cellulases was followed in 4 cultures of higher fungi (agrocybe cylindracea, len tinus tigrinus, pleurotus ostreatus, ramaria formosa) cultivated on various substrates under different conditions. stationary cultivation was more suitable than the submerged one. addition of carboxymethy cellulose (cmc) was more suitable than addition of glucose. the cellulase activity in the presence of cmc was higher after a 12-d cultivation than after a 23-d period. pine sawdust was most effective ... | 1981 | 7262712 |
| metabolism of phenanthrene by the white rot fungus pleurotus ostreatus. | the white rot fungus pleurotus ostreatus, grown for 11 days in basidiomycetes rich medium containing [14c] phenanthrene, metabolized 94% of the phenanthrene added. of the total radioactivity, 3% was oxidized to co2. approximately 52% of phenanthrene was metabolized to trans-9,10-dihydroxy-9,10-dihydrophenanthrene (phenanthrene trans-9,10-dihydrodiol) (28%), 2,2'-diphenic acid (17%), and unidentified metabolites (7%). nonextractable metabolites accounted for 35% of the total radioactivity. the me ... | 1996 | 8779594 |
| effect of oyster mushroom (pleurotus ostreatus) and its ethanolic extract in diet on absorption and turnover of cholesterol in hypercholesterolemic rat. | the effect of the diet containing 5% of powdered oyster mushroom (pleurotus ostreatus) or an equivalent amount of mushroom ethanolic extract on cholesterol content in serum and liver, on its distribution in lipoproteins, absorption and turnover was studied in male wistar rats (initial body weight about 70 g) fed a diet with 0.3% cholesterol. 12 weeks of feeding with whole oyster mushroom or mushroom extract reduced cholesterol level in serum by 52 and 33%, respectively. however, cholesterol cont ... | 1996 | 8810086 |
| oyster mushroom (pleurotus ostreatus) reduces the production and secretion of very low density lipoproteins in hypercholesterolemic rats. | oyster mushroom (pleurotus ostreatus) reduced the production and secretion of nascent very low density lipoproteins in hypercholesterolemic rats. in male wistar rats (initial body weight about 70 g) fed a semisynthetic diet with 0.3% of cholesterol, the addition of 5% of powdered oyster mushroom (pleurotus ostreatus) to the diet reduced after 8 weeks the level of serum cholesterol (by 36%) and accumulation of cholesterol and triglycerides in liver (by 51 and 32%, respectively). the decreased lev ... | 1996 | 8896287 |
| the integrative transformation of pleurotus ostreatus using bialaphos resistance as a dominant selectable marker. | a plasmid plc-bar containing the bialaphos resistance gene derived from streptomyces hygroscopicus between the lentinus edodes ras gene promoter and pria gene terminator was constructed. when protoplasts of pleurotus ostreatus were mixed with the plasmid dna in the presence of polyethylene glycol and cacl2, bialaphos-resistant colonies were obtained. this indicated that transformation was successful. southern blot analysis of total dnas from transformants showed that the introduced plasmid dna w ... | 1996 | 8901106 |
| a lectin from an edible mushroom pleurotus ostreatus as a food intake-suppressing substance. | in an experiment in which rats were allowed free access to food and water, the rats did not eat the diet containing a mushroom pleurotus ostreatus even if they were emaciated. a p. ostreatus lectin (pol) was isolated from the mushroom as the food intake-suppression principle. in hemagglutination inhibition assays, me-alphagalnac was the most potent inhibitor among the monosaccharides tested. among all the sugars tested, 2'-fucosyllactose (fucalpha1-->2galbeta1-->4glc) was the strongest inhibitor ... | 2000 | 10779681 |
| the nucleotide sequences of the 5s rrnas of four mushrooms and their use in studying the phylogenetic position of basidiomycetes among the eukaryotes. | the nucleotide sequences of the 5 s ribosomal rnas of the mushrooms russula cyanoxantha, pleurotus ostreatus, agaricus edulis, and auricularia auricula-judae were determined. the sequences fit in a universal five-helix secondary structure model for 5 s rna. as in most other 5 s rnas, some helical areas contain non-standard base pairs. a clustering method was used to reconstruct an evolutionary tree from 82 eukaryotic 5 s rna sequences. it allows to make a choice between alternative systematic cl ... | 1983 | 6856478 |
| the effect of white-rot basidiomycetes on chemical composition and in vitro digestibility of wheat straw. | five white-rot basidiomycetes were evaluated for their potential to improve ruminal degradation of wheat straw. polyorus brumalis, lyophyllum ulmarium iii, trametes gibbosa, pleurotus ostreatus, and a pleurotus ostreatus mutant were incubated on wheat straw for 30 d at 28 degrees c. detergent fiber, crude protein and in vitro dry matter digestibility (ivdmd) were determined. the results showed increasing crude protein and ash contents in fungus-treated straw. ivdmd values were increased in straw ... | 1996 | 9090827 |
| biochemical and molecular characterization of a manganese peroxidase isoenzyme from pleurotus ostreatus. | in this study we purified and investigated the catalytic properties of a manganese peroxidase isoenzyme produced by the fungus pleurotus ostreatus in liquid medium with peptone as nitrogen source. the isoenzyme was purified to homogeneity by chromatography on bio-rad q-cartridge, sephacryl s-200 and mono-q with activity yield of 59% and a purification factor of 36. the p. ostreatus mnp obtained had the same pi (3.75) and n-terminal sequence as mnp-1 of pleurotus eryngii produced in the same medi ... | 1997 | 9165096 |
| role of apoptosis, proliferating cell nuclear antigen and p53 protein in chemically induced colon cancer in rats fed corncob fiber treated with the fungus pleurotus ostreatus. | the role of apoptosis, proliferative cell nuclear antigen (pcna) and p53 protein in the preventive effects of dietary fiber treated with the fungus pleurotus ostreatus on rat-colon tumorigenesis was studied. tumors were induced by five subcutaneous injections of 1,2-dimethylhydrazine (dmh), 20 mg/kg rat, once a week. rats were fed a semi-synthetic fiberfree diet (control) or a high-fiber diet (15%) derived from corncob treated or non-treated with the fungus. the rats we sacrificed 24 weeks after ... | 1997 | 9216672 |
| [regulation of cholesterol metabolism with dietary addition of oyster mushrooms (pleurotus ostreatus) in rats with hypercholesterolemia]. | it is generally accepted that lowering of serum cholesterol levels reduces the risk of atherosclerosis. identification and characterization of natural substances with hypocholesterolemic activity useful in dietetic prevention or treatment of hypercholesterolemia is still relevant in countries with persistent progression of hypercholesterolemia. addition of oyster mushroom (pleurotus ostreatus), an industrially produced wood-rotting fungus, to the diet effectively reduced cholesterol accumulation ... | 1997 | 9221192 |
| role of oyster mushroom (pleurotus florida) as hypocholesterolemic/antiatherogenic agent. | feeding of pleurotus to hypercholesterolemic rabbits resulted in lowering of total lipids, total cholesterol and glyceride levels of plasma and liver whereas heart lipids were not affected. hdl cholesterol/total cholesterol and hdl cholesterol/ldl cholesterol ratios increased in experimental animals proving antiatherogenic potential of this mushroom. the effect was further supported by the severity of tissue damage as evidenced by histopathological studies. an increase in bile acid excretion cou ... | 1997 | 9475042 |
| cloning and characterization of the gene encoding the iron-sulfur protein of succinate dehydrogenase from pleurotus ostreatus. | genomic and cdna fragments encoding the iron-sulfur protein (ip) subunit of dehydrogenase (ec 1.3.99.1) have been cloned from the edible basidiomycetous fungus, pleurotus ostreatus. the gene is interrupted by five introns and is predicted to encode a polypeptide of 268 amino acid residues. sequence comparison with the ip subunit from other species identified three conserved cysteine-rich clusters. one of these contains a critical histidine residue implicated in carboxin sensitivity in the hetero ... | 1998 | 9524211 |
| molecular breeding of the basidiomycete coprinus cinereus strains with high lignin-decolorization and -degradation activities using novel heterologous protein expression vectors. | two chromosome-integrating vectors, plc1 and plc2, were used. the former is the puc19-based vector carrying the lentinus edodes ras gene promoter and pria gene terminator, and the latter is the pbr322-based vector carrying the promoter and terminator of the pria gene. the manganese (ii) peroxidase (mnp) cdna (mnpc) derived from pleurotus ostreatus was fused between the promoter and terminator of plc1 and plc2, yielding the recombinant plasmids plc1-mnp and plc2-mnp. these plasmids were introduce ... | 1998 | 9581292 |
| protease activity in cockroach and basidiomycete allergen extracts. | inherent proteolytic activity was estimated in cockroach and basidiomycete extracts by quantifying acid soluble peptides that were released by incubating extracts with 1% bovine serum albumin as measured by lowry (sigma). reference proteases released 740 (proteinase k, 0.1 u), 248 (trypsin, 1.0 u), and 533 micrograms/ml (pronase, 0.5 u) of soluble peptides. american whole body cockroach extract (0.1 mg dry weight) released 330 micrograms/ml of soluble peptides, representing 13 trypsin equivalent ... | 1993 | 8224832 |
| metabolism of pah by fungi and correlation with extracellular enzymatic activities. | the activity to metabolize the polycyclic aromatic hydrocarbons (pah) phenanthrene, anthracene, pyrene, fluorene and fluoranthene by trametes versicolor, pleurotus ostreatus (white rot fungi), laetiporus sulphureus, daedaela quercina, flamulina velutipes (brown rot fungi), marasmiellus sp. (litter decaying fungus) and penicillium sp. m 1 (isolated from a pah contaminated soil sample) were compared. screening methods for the presence of exoenzymes (peroxidases, polyphenoloxidases, "radical genera ... | 1993 | 8229670 |
| a nucleotide sequence involved in replicative transformation of a filamentous fungus. | replicative plasmids generated through in-vivo recombination have been identified among transformants of the fungus pleurotus ostreatus. in addition to sequences from a standard selection vector (pan7-1), these recombinant plasmids contain recombined sequences of chromosomal origin conferring replicative potential upon the vector. one such recombined sequence, an 1148-bp insert into plasmid pp01, has been characterized. this sequence has been analyzed for secondary structural features as well as ... | 1993 | 8358817 |
| dose-dependent hypocholesterolaemic effect of oyster mushroom (pleurotus ostreatus) in rats. | a highly significant negative correlation (r=-0.981, p < 0.001) between the amount of oyster mushroom (pleurotus ostreatus) in the diet and cholesterol levels in the serum has been found in male wistar rats fed shortly after weaning by a a diet with 0.3% cholesterol. the addition of 1.0, 2.5 and 5.0% of oyster mushroom to the diet reduced the levels of serum cholesterol by 11, 31 and 46%, respectively. the diet containing 5% of oyster mushroom suppressed cholesterol accumulation in the liver and ... | 1997 | 9728501 |
| identification, characterization, and in situ detection of a fruit-body-specific hydrophobin of pleurotus ostreatus. | hydrophobins are small (length, about 100 +/- 25 amino acids), cysteine-rich, hydrophobic proteins that are present in large amounts in fungal cell walls, where they form part of the outermost layer (rodlet layer); sometimes, they can also be secreted into the medium. different hydrophobins are associated with different developmental stages of a fungus, and their biological functions include protection of the hyphae against desiccation and attack by either bacterial or fungal parasites, hyphal a ... | 1998 | 9758836 |
| investigation into pcb biodegradation using uniformly 14c-labelled dichlorobiphenyl. | biodegradation of polychlorinated biphenyls (pcbs) in soil is considered to be very complex due to various physico-chemical factors involved. isotope labelling technique is the best to trace fate of the xenobiotic in the environment. in this work, the uniformly 14c-labelled pcb congener 11 (3,3'-chlorobiphenyl) was chosen as a low chlorinated coplanar biphenyl which was assumed to be readily degraded by microorganisms. pleurotus ostreatus and two pseudomonas species, representing white rot fungi ... | 1998 | 10089593 |
| [protein quality of three strains of mexican mushrooms (pleurotus ostreatus)]. | the protein quality of fruits bodies of three pleurotus ostreatus mexican strains (inireb-8, cdbb-h-896 and cdbb-h-897) was evaluated. the protein concentration (nx4.38) ranged from 17.26 to 19.97 g/100 g dry weight; chemical scores were between 74 and 93% with available lysine as a first limiting amino acid in either inireb-8 and cdbb-h-896 strains or leucine in cdbb-h-897 strain. the nutritional evaluation revealed 67.75 to 68.38% in vitro digestibility. relative protein values were from 100.0 ... | 1999 | 10412512 |
| first chromatographic isolation of an antifungal thaumatin-like protein from french bean legumes and demonstration of its antifungal activity. | a protein, with a molecular weight of 20 kda, and an n-terminal sequence analogous to those of thaumatin-like proteins (tlps) and thaumatins, was first isolated from the legume of the french bean phaseolus vulgaris cv kentucky wonder using a simple procedure involving affinity and ion exchange chromatography. the protein was adsorbed on both cm-sepharose and affi-gel blue gel. it was the first leguminous tlp-like protein demonstrated to exert antifungal activity against fusarium oxysporum, pleur ... | 1999 | 10486265 |
| extracellular oxidative enzyme production and pah removal in soil by exploratory mycelium of white rot fungi. | selected strains of three species of white rot fungi, pleurotus ostreatus, phanerochaete chrysosporium and trametes versicolor, were grown in sterilized soil from straw inocula. the respective colonization rates and mycelium density values decreased in the above mentioned order. three- and four-ringed pahs at 50 ppm inhibited growth of fungi in soil to some extent. the activities of fungal mnp and laccase (units per g dry weight of straw or soil), extracted with 50 mm succinate-lactate buffer (p ... | 1999 | 10492884 |
| hypocholesterolemic and antiatherogenic effect of oyster mushroom (pleurotus ostreatus) in rabbits. | the addition of 10% dried fruiting bodies of oyster mushroom (pleurotus ostreatus) to the diet containing 1% of cholesterol reduced serum cholesterol levels by 65% and cholesterol content in liver, heart, long extensor muscle and aorta of male rabbits (chinchilla) by 60, 47, 25 and 80%, respectively. the decrease in total serum cholesterol was affected primarily (by 70%) by reduced cholesterol content in very-low-density lipoproteins (vldl) while the contribution of high density lipoproteins (hd ... | 1999 | 10555301 |
| [the oyster mushroom (pleurotus ostreatus) effectively prevents the development of atherosclerosis in rabbits]. | the addition of 10% dried fruiting bodies of the oyster mushroom (pleurotus ostreatus) to the diet containing 1% of cholesterol reduced serum cholesterol levels by 65% and cholesterol content in the liver, heart, long extensor muscle and aorta of male rabbits (chinchilla) by 60; 47; 25 and 79%, respectively. oyster mushroom diet reduced the content of conjugated dienes by 60-70% in the plasma, erythrocytes and liver. however, it did not significantly affect the activities of antioxidant enzymes. ... | 1999 | 10566243 |
| isolation and transformation of uracil auxotrophs of the edible basidiomycete pleurotus ostreatus. | uracil auxotrophs of pleurotus ostreatus were isolated using the selectable marker, resistance to 5'-fluoro-orotic acid (5'-foa). two of the nine uracil auxotrophs obtained were transformed to prototrophy using plasmid ptrura 3-2 that contains the orotidine monophosphate decarboxylase (ura3) gene from trichoderma reesei. southern blot analyses of the transformants showed that the transforming dna had integrated into the genome of the protoplasts. using 2 x 10(7) protoplasts, this system gave a t ... | 1999 | 10585542 |
| [influence of culture conditions on ligninolytic activity of stereum hirsutum]. | stereum hirsutum bafc 2234 was tested for growth kinetics and ligninolytic enzyme production. the strain showed weak coincidences with widely studied organisms (phanerochaete chrysosporium, pleurotus ostreatus) when the response to nitrogen sources was assayed. this could be interpreted as a different regulation to nitrogen metabolism for s. hirsutum bafc 2234. alternative carbon sources and the addition of veratryl alcohol and wood extracts were also tested showing partial correspondence with o ... | 2000 | 10785937 |
| the molecular genetics of cultivated mushrooms. | the types, economic significance and methods of production of the principal cultivated mushrooms are described in outline. these organisms are all less than ideal for conventional genetic analysis and breeding, so molecular methods afford a particular opportunity to advance our understanding of their biology and potentially give the prospect of improvement by gene manipulation. the sequences described are limited to those found in genbank by august 1999. the gene sequences isolated from the whit ... | 2000 | 10907549 |
| sativin: a novel antifungal miraculin-like protein isolated from legumes of the sugar snap pisum sativum var. macrocarpon. | an antifungal protein designated sativin was isolated from the legumes of the sugar snap (also known as honey pea) pisum sativum var. macrocarpon. the procedure entailed extraction, affinity chromatography on affi-gel blue gel and ion exchange chromatography on cm-sepharose. the protein exhibited a molecular weight of 38 kda in sds-polyacrylamide gel electrophoresis. it possessed an n-terminal amino acid sequence which showed similarity to those of miraculin (a sweet protein) and pisavin (a ribo ... | 2000 | 10968407 |
| a new lectin with highly potent antihepatoma and antisarcoma activities from the oyster mushroom pleurotus ostreatus. | a dimeric lectin, composed of subunits with a molecular weight of 40 and 41 kda, respectively, and demonstrating similarity in n-terminal sequence to each other and to aleuria aurantia lectin, was isolated from fresh fruiting bodies of the edible mushroom pleurotus ostreatus. the lectin was unadsorbed on deae-cellulose in 10 mmol/l phosphate buffer (ph 7.5), subsequently adsorbed on cm-cellulose in 10 mmol/l nh(4)oac (ph 5.4), and came off in the first peak from a superose 12 column during fast ... | 2000 | 10973803 |
| isolation of a novel ubiquitin-like protein from pleurotus ostreatus mushroom with anti-human immunodeficiency virus, translation-inhibitory, and ribonuclease activities. | a glycoprotein, with a ubiquitin-like n-terminal sequence, has been prepared from an extract of fruiting bodies of the mushroom pleurotus ostreatus, using a procedure which included ion exchange chromatography on deae-cellulose, affinity chromatography on affi-gel blue gel, ion exchange chromatography on sp-sepharose and mono q and gel filtration on superdex 75. it exhibited a molecular weight of 12.5 kda and was unadsorbed on deae-cellulose and mono q, but adsorbed on affi-blue gel and sp-sepha ... | 2000 | 11027517 |
| application of siderotyping for characterization of pseudomonas tolaasii and "pseudomonas reactans" isolates associated with brown blotch disease of cultivated mushrooms. | pyoverdine isoelectric focusing analysis and pyoverdine-mediated iron uptake were used as siderotyping methods to analyze a collection of 57 northern and central european isolates of p. tolaasii and "p. reactans." the bacteria, isolated from cultivated agaricus bisporus or pleurotus ostreatus mushroom sporophores presenting brown blotch disease symptoms, were identified according to the white line test (w. c. wong and t. f. preece, j. appl. bacteriol. 47:401-407, 1979) and their pathogenicity to ... | 2000 | 11055932 |
| structural and kinetic characterization of native laccases from pleurotus ostreatus, rigidoporus lignosus, and trametes trogii. | a comparative study has been performed on five native laccases purified from the three basidiomycete fungi pleurotus ostreatus, rigidoporus lignosus, and trametes trogii to relate their different catalytic capacities to their structural properties. spectroscopic absorption features and epr spectra at various ph values of the five enzymes are very similar and typical of the blue oxidases. the analysis of the dependence of kinetic parameters on ph suggested that a histidine residue is involved in ... | 2001 | 11565899 |
| a new broad-spectrum protease inhibitor from the entomopathogenic bacterium photorhabdus luminescens. | a new protease inhibitor was purified to apparent homogeneity from a culture medium of photorhabdus luminescens by ammonium sulfate precipitation and preparative isoelectric focusing followed by affinity chromatography. ph. luminescens, a bacterium symbiotically associated with the insect-parasitic nematode heterorhabditis bacteriophora, exists in two morphologically distinguishable phases (primary and secondary). it appears that only the secondary-phase bacterium produces this protease inhibito ... | 2000 | 11101672 |
| characterization of fungal 17beta-hydroxysteroid dehydrogenases. | to promote understanding of the evolution of the steroid hormone signalling and hydroxysteroid dehydrogenases (hsds), comparative characterization of fungal 17beta-hsds was performed. constitutive 17beta-hsd activity was determined in cytosols of the fungi: cochliobolus lunatus, pleospora herbarum, fusarium lini, trichoderma viride, mucor spinosus, rhizopus nigricans and pleurotus ostreatus. the reaction equilibrium in all species except p. ostreatus was shifted towards reduction. the preferenti ... | 2000 | 11126752 |
| stable transformation of pleurotus ostreatus to hygromycin b resistance using lentinus edodes gpd expression signals. | it was reported that pleurotus ostreatus was transformed unstably using recombinant plasmids containing a hygromycin b phosphotransferase gene (hph) under the control of aspergillus nidulans expression signals, and that the plasmids were maintained extrachromosomally in the transformants. here we report a stable and integrative transformation of the fungus to hygromycin b resistance, using a recombinant hph fused with lentinus edodes glyceraldehyde-3-phosphate dehydrogenase expression signals. r ... | 2001 | 11601618 |
| effect of oyster mushroom (pleurotus ostreatus) on pathological changes in dimethylhydrazine-induced rat colon cancer. | the effect of 5% of dried oyster mushroom (pleurotus ostreatus) in the diet on the dimethylhydrazine (dmh)-induced colon carcinogenesis was studied in male wistar rats. dmh in a dose of 20 mg/kg of body weight was applied to animals once a week during a period of 12 weeks. mushroom diet was applied either after treatment with dmh for another 21 weeks or during the whole experiment. mushroom diet reduced significantly the incidence of lymphoid hyperplasia foci when mushroom was supplemented durin ... | 1998 | 9538185 |
| biodegradation of polycyclic aromatic hydrocarbons (pahs) by native microflora and combinations of white-rot fungi in a coal-tar contaminated soil. | four white-rot fungi (phanerochaete chrysosporium imi 232175, pleurotus ostreatus from the university of alberta microfungus collection imi 341687, coriolus versicolor imi 210866 and wye isolate #7) and all possible combinations of two or more of these fungi, were incubated in microcosms containing wheat straw and non-sterile coal-tar contaminated soil to determine their potential to degrade polycyclic aromatic hydrocarbons (pahs). biotic and abiotic controls were prepared similarly and pah conc ... | 2001 | 11131793 |