| comparison of 5.8s ribosomal dna sequences among the basidiomycetous yeast genera cystofilobasidium, filobasidium and filobasidiella. | nucleotide sequences obtained from regions of the ribosomal dna repeat were compared by phylogenetic methods and combined with a statistical evaluation to clarify the relationships among the genera cystofilobasidium, filobasidium (f.) and filobasidiella (fl.), to assess the affinity of filobasidiella neoformans and filobasidiella depauperata, and to compare the varieties of fl. neoformans. with appropriate primers, the nuclear 18s, 5.8s and internal transcribed spacer (its) regions of the riboso ... | 1992 | 1355536 |
| on the taxonomy of the entomogenous fungus filobasidiella arachnophila. | the taxonomic status of filobasidiella arachnophila malloch et al. was investigated. the carbohydrate profile of two strains revealed basidiomycetous affinities. however, the vast majority of the mycelial cells are monokaryotic, demonstrating that f. arachnophila is not a typical basidiomycete. the morphological resemblance to the two teleomorph species of filobasidiella is noteworthy and therefore the accommodation in filobasidiella is maintained. f. arachnophila proved to be identical with asp ... | 1983 | 6651289 |
| unique oligonucleotide primers in pcr for identification of cryptococcus neoformans. | on the basis of a comparison of rrna sequences coding the internal transcribed spacer (its) and 5.8s regions of filobasidiella neoformans, the teleomorph of cryptococcus neoformans, and members of its most closely related taxa, unique oligonucleotides were designed for the specific amplification of dna only from c. neoformans. various combinations of these primers, designated cn4, cn5, and cn6, and the previously described fungal primers its1 and its2 were tested in pcr for their ability to ampl ... | 1994 | 8126194 |
| phylogenetic relationships of cryptococcus neoformans and some related basidiomycetous yeasts determined from partial large subunit rrna sequences. | the genus cryptococcus was found to be heterogeneous on the basis of partial rrna sequences. the human-pathogenic species c. neoformans, comprising 4 serotypes and having filobasidiella neoformans and f. bacillispora as teleomorphs, was found at a relatively large distance from filobasidium. serotypes b and c had identical sequences, while in a and d they were different, with d closer to b and c than to a. filobasidiella depauperata, which lacks a yeast-like anamorph, clustered with f. neoforman ... | 1993 | 8259834 |
| detection of cryptococcus neoformans dna in tissue samples by nested and real-time pcr assays. | two pcr protocols targeting the 18s rrna gene of cryptococcus neoformans were established, compared, and evaluated in murine cryptococcal meningitis. one protocol was designed as a nested pcr to be performed in conventional block thermal cyclers. the other protocol was designed as a quantitative single-round pcr adapted to lightcycler technology. one hundred brain homogenates and dilutions originating from 20 icr mice treated with different azoles were examined. a fungal burden of 3 x 10(1) to 2 ... | 2002 | 11874894 |
| phylogeny and phenotypic characterization of pathogenic cryptococcus species and closely related saprobic taxa in the tremellales. | the basidiomycetous yeasts cryptococcus neoformans and cryptococcus gattii are closely related sibling species that cause respiratory and neurological disease in humans and animals. within these two recognized species, phylogenetic analysis reveals at least six cryptic species defined as molecular types (vni/ii/b, vniv, vgi, vgii, vgiii, and vgiv) that comprise the pathogenic cryptococcus species complex. these pathogenic species are clustered in the filobasidiella clade within the order tremell ... | 2009 | 19151324 |
| morphological and genomic characterization of filobasidiella depauperata: a homothallic sibling species of the pathogenic cryptococcus species complex. | the fungal species cryptococcus neoformans and cryptococcus gattii cause respiratory and neurological disease in animals and humans following inhalation of basidiospores or desiccated yeast cells from the environment. sexual reproduction in c. neoformans and c. gattii is controlled by a bipolar system in which a single mating type locus (mat) specifies compatibility. these two species are dimorphic, growing as yeast in the asexual stage, and producing hyphae, basidia, and basidiospores during th ... | 2010 | 20224779 |