| use of a mouse model to evaluate clinical and environmental isolates of sporothrix spp. from the largest u.s. epidemic of sporotrichosis. | five clinical and 69 environmental isolates from the largest u.s. epidemic of sporotrichosis were evaluated in nylar male mice following intravenous injection of 5 x 10(6) to 2 x 10(8) conidia per mouse. the clinical isolates and eight environmental isolates produced 100% mortality in groups of three mice each between 12 and 24 days after injection. these virulent isolates grew at 37 degrees c, were dematiaceous by virtue of melanin (melanized) on permissive media (e.g., potato dextrose agar), p ... | 1992 | 1572983 |
| isolation and characterization of sporothrix schenckii from clinical and environmental sources associated with the largest u.s. epidemic of sporotrichosis. | the largest recorded epidemic of sporotrichosis in the united states occurred in 1988 and involved a total of 84 cases in 15 states. all cases were associated with wisconsin-grown sphagnum moss. twenty-one clinical isolates of sporothrix schenckii and 69 environmental isolates of sporothrix spp. from the epidemic were characterized and compared. the environmental isolates were recovered from 102 samples of sphagnum moss and other material by using direct plating techniques. characteristics exami ... | 1991 | 1864926 |
| novel antigenic determinants from peptidorhamnomannans of sporothrix schenckii. | antisera were raised in rabbits against acetone-dried yeast-like and mycelium forms of sporothrix schenckii. these antisera were tested for immunoprecipitation of peptidorhamnomannans isolated from both cell types. both antisera reacted strongly with s.schenckii peptidorhamnomannans, but the reactions were weak with beta-eliminated peptidopolysaccharides. these antisera did not recognize the saccharomyces cerevisiae mannoprotein, and reacted poorly with ceratocystis (ophiostoma) stenoceras cell ... | 1994 | 7524826 |
| [dermatophytes do not produce sialidase in vitro]. | sialidase (ec 3.2.1.18) is a pathogenicity factor of many microorganisms, and may also play a role in adhesion of dermatophytes to the epithelia of their hosts by the hydrolytical cleavage of terminal, negatively charged sialic acids of glycoconjugates on the cell surfaces, thus allowing fungal lectins to bind to the subterminal sugars. therefore, 116 strains of seven species of dermatophytes were investigated for sialidase production. two highly sensitive, quantitative sialidase assays were app ... | 1997 | 9417509 |
| lack of sialidase activity in candida albicans and candida glabrata. | because several micro-organisms having close contact to animal hosts and man produce sialidase (ec 3.2.1.18) as a tool for adhesion and invasion, we investigated two candida species for the presence of this enzyme. two sensitive assays, a fluorometric test with 4-methylumbelliferyl-alpha-d-n-acetylneuraminic acid and a radiometric test with 3h-labelled sialyllactitol as sialidase substrates, were applied to detect sialidase activity. none of 40 candida albicans and 10 c. glabrata strains grown i ... | 1999 | 10394845 |
| ophiostoma species (ascomycetes: ophiostomatales) associated with bark beetles (coleoptera: scolytinae) colonizing pinus radiata in northern spain. | bark beetles (coleoptera: scolytinae) are known to be associated with fungi, especially species of ophiostoma sensu lato and ceratocystis. however, very little is known about these fungi in spain. in this study, we examined the fungi associated with 13 bark beetle species and one weevil (coleoptera: entiminae) infesting pinus radiata in the basque country of northern spain. this study included an examination of 1323 bark beetles or their galleries in p. radiata. isolations yielded a total of 920 ... | 2007 | 17668036 |
| taxonomy and phylogeny of new wood- and soil-inhabiting sporothrix species in the ophiostoma stenoceras-sporothrix schenckii complex. | sporothrix, one of the anamorph genera of ophiostoma, includes the important human pathogen s. schenckii and various fungi associated with insects and sap stain of wood. a survey of fungi from wood utility poles in south africa yielded two distinct groups of sporothrix isolates from different geographical areas. dna sequence and morphological data derived in this study showed that isolates in these groups represent two novel species in the s. schenckii-o. stenoceras species complex. a new specie ... | 2008 | 18833758 |
| two-stage gas-phase bioreactor for the combined removal of hydrogen sulphide, methanol and alpha-pinene. | biological treatment systems have emerged as cost-effective and eco-friendly techniques for treating waste gases from process industries at moderately high gas flow rates and low pollutant concentrations. in this study, we have assessed the performance of a two-stage bioreactor, namely a biotrickling filter packed with pall rings (btf, 1st stage) and a perlite + pall ring mixed biofilter (bf, 2nd stage) operated in series, for handling a complex mixture of hydrogen sulphide (h2s), methanol (ch3o ... | 2009 | 19950468 |
| sporothrix brunneoviolacea and sporothrix dimorphospora, two new members of the ophiostoma stenoceras-sporothrix schenckii complex. | sporothrix inflata is a saprobic member of the ophiostoma stenoceras-sporothrix schenckii species complex, reported mainly from soil. ophiostoma bragantinum, an ascomycete described from brazil, has been proposed as its possible teleomorph. previous studies revealed that sporothrix inflata is phenotypically and genetically variable, suggesting the existence of cryptic species. during a continued survey on the biodiversity of microfungi from different countries, seven isolates morphologically sim ... | 2010 | 20943519 |
| two new ophiostoma species with sporothrix anamorphs from austria and azerbaijan. | the genus ophiostoma includes numerous species of primarily insect-vectored, wood-staining fungi. several anamorph genera that differ in their micronematous or macronematous conidiogenous cells have been associated with ophiostoma species. among the former group, sporothrix is associated with many species and is characterized by conidiogenous cells that arise laterally or terminally from any place on the hyphae and produce nonseptate conidia on sympodially developing denticles. the purpose of th ... | 2004 | 21148906 |
| phylogeny of the ophiostoma stenoceras-sporothrix schenckii complex. | ophiostoma stenoceras is a well-known sapwood-colonizing fungus occurring on some coniferous and hardwood hosts in the northern hemisphere. in the southern hemisphere, the fungus has been reported only from new zealand. the human pathogen, sporothrix schenckii, has been suggested to be the anamorph of o. stenoceras. the aim of this study was to gain a better understanding of the phylogenetic relationship between these two species. the study also provided the opportunity to confirm the identity o ... | 2003 | 21156632 |
| one-stage biotrickling filter for the removal of a mixture of volatile pollutants from air: performance and microbial community analysis. | the biodegradation of gas-phase mixtures of methanol, α-pinene and h2s was examined in a biotrickling filter (btf), inoculated with a microbial consortium composed of an autotrophic h2s-degrading culture, and pure strains of candida boidinii, rhodococcus erythropolis, and ophiostoma stenoceras. the inlet concentrations of methanol, α-pinene and h2s varied from 0.05 to 3.3 gm(-3), 0.05 to 2.7 gm(-3), and 0.01 to 1.4 gm(-3), respectively, at empty bed residence times (ebrt) of either 38 or 26s. th ... | 2013 | 23612184 |
| wounds on rapanea melanophloeos provide habitat for a large diversity of ophiostomatales including four new species. | rapanea melanophloeos, an important canopy tree in afromontane forests, is commonly utilised for medicinal bark harvesting. wounds created from these activities provide entrance for many fungi, including arthropod-associated members of the ophiostomatales and microascales (ophiostomatoid fungi). in this study we assessed the diversity of wound-associated ophiostomatales on storm-damaged r. melanophloeos trees in the afromontane forests of south africa. five species were identified based on micro ... | 2016 | 27022984 |