| detection of mutagens produced by fungi with the salmonella typhimurium assay. | forty-one fungal isolates (one isolate per species) representing common plant pathogens and food crop contaminants were grown on sterile, polished rice and assayed for mutagenic activity in the salmonella typhimurium-microsome system. initially, single doses of aqueous and chloroform extracts of the moldy rice were assayed against the ta100 tester strain by incorporating extracts into the growth medium and by applying small quantities on disks placed on the agar surface. suspected activity was e ... | 1978 | 354528 |
| procedures for obtaining sectional views of fungal fructifications by scanning electron microscopy. | procedures for sectioning fungal fructifications in host tissues or on artificial media are described, which allow observation of internal structures by scanning electron microscopy. perithecia of ceratocystis fimbriata and phyllachora graminis, and telia of puccinia xanthii showed excellent preservation of exposed structures in sections which were osmium-coated before being dried. while similar preservation was obtained in sectioned acervuli of lecanosticta acicola and marssonia juglandis and i ... | 1976 | 974917 |
| [biochemical aspects of resistance mechamism of mango (mangifera indica l. to ceratocystis fimbriata ell. & hals]. | | 1974 | 4461018 |
| 8-hydroxy-6-methoxy-3-methylisocoumarin and other metabolites of ceratocystis fimbriata. | | 1969 | 5772578 |
| enzymic changes associated with induced and natural resistance of sweetpotato to ceratocystis fimbriata. | | 1967 | 6039500 |
| isolation of furanoterpene-containing particles from ceratocystis fimbriata-infected sweet potato root tissue. | furanoterpene-containing particles were isolated by centrifugation on a discontinuous ficoll density gradient from a homogenate of the non-infected tissue adjacent to the infected region of ceratocystis fimbriata-infected sweet potato root tissue. the particles were recovered at a relatively high ratio in the 2% ficoll fraction, in which there was no contamination by mitochondria and only little by endoplasmic reticulum judging from the distribution of the activities of their marker enzymes and ... | 1984 | 6099359 |
| the fine structure of ascospore shape and development in ceratocystis fimbriata. | ascospore development in ceratocystis fimbriata ell. & halst. commenced in an eight-nucleate ascus. a single vesicle formed along the periphery of the ascus from fragments of ascospore delimiting membranes, surrounded all eight nuclei and eventually invaginated, first forming pouches with open ends, then finally enclosing each of the eight nuclei in a separate sac, thus delimiting ascospores. pairing of the ascospores followed and brim formation occurred at the contact area between two ascospore ... | 1995 | 7574547 |
| relatedness of pseudallescheria, scedosporium and graphium pro parte based on ssu rdna sequences. | genetic relatedness of 10 strains of opportunistic human pathogenic fungi in the genera pseudallescheria and scedosporium and related fungi including petriella setifera, graphium tectonae and ceratocystis fimbriata, were tested by sequencing the entire ssu rdna. from these molecular data, the imperfect genus scedosporium can be divided in two, s. prolificans being separable from the s. apiospermum complex. nearest sexual relatives of s. prolificans seem to be in the microascalean genus petriella ... | 1997 | 9467105 |
| purification, characterization, and amino acid sequence of cerato-platanin, a new phytotoxic protein from ceratocystis fimbriata f. sp. platani. | a new phytotoxic protein (cerato-platanin) of about 12.4 kda has been identified in culture filtrates of the ascomycete ceratocystis fimbriata f. sp. platani, the causal agent of canker stain disease. the toxicity of the pure protein was bioassayed by detecting the inducing necrosis in tobacco leaves. the pure protein also elicited host synthesis of fluorescent substances in tobacco and plane (platanus acerifolia) leaves. we purified the protein from culture medium to homogeneity. its complete a ... | 1999 | 10455173 |
| electron transport system in ceratocystis fimbriata. | | 1964 | 14116613 |
| ceratocystis infection in sweet potato: its effect on proteins, isozymes, and acquired immunity. | changes take place in the protein and isozyme patterns of tissue adjacent to cut surfaces of sweet potato roots infected by the fungus ceratocystis fimbriata. chromatography and gel electrophoresis of extracts from sections cut at known distances from a plane of infection showed that inoculation with a pathogenic or nonpathogenic isolate produced similar changes in several proteins and enzymes. inoculation of a susceptible variety of sweet potato with the nonpathogenic isolate induced in a thin ... | 1964 | 14199715 |
| [study of ceratocystis fimbriata toxins determination from sweet potato by high performance thin-layer chromatography]. | the extract solution from sweet potato was washed with 5% na2co3 and followed with distilled water and dried with dehydrated sodium sulfate (na2so4). the ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a silica gel g thin-layer plate developed with different solvent systems. pure ipomeamarone and ipomeamaronol were used as standards. the resolution was the best in the solvent system of petroleum ether-e ... | 2003 | 14535108 |
| cerato-platanin protein is located in the cell walls of ascospores, conidia and hyphae of ceratocystis fimbriata f. sp. platani. | cerato-platanin (cp), a protein of about 12.4 kda from ceratocystis fimbriata f. sp. platani (cfp), accumulated in the mycelium and was located in the cell walls of cfp ascospores, hyphae and conidia suggesting that this protein had a role in forming the fungal cell wall apart from the already known fact that it is secreted early in culture and elicits phytoalexin synthesis and/or plant cell death. the finding was obtained with three immunological techniques: a quantitative elisa which determine ... | 2004 | 15063505 |
| a pervaporation-bio-hybridreactor (pbhr) for improved aroma biosynthesis with submerged culture of ceratocystis fimbriata. | in the present study a pervaporation-bio-hybridreactor was investigated for the improved biotechnological production of volatile flavour compounds. the mixture of esters and alcohols produced by the fungus ceratocystis fimbriata had a fruity, banana-like odour and can legally be defined as "natural flavour". increasing consumer demand and high market prices make the production of bioflavours an attractive alternative to chemical synthesises. however, the yield of biotechnological flavour product ... | 2003 | 15296172 |
| genetic variation in eastern north american and putatively introduced populations of ceratocystis fimbriata f. platani. | the plant pathogenic fungus ceratocystis fimbriata f. platani attacks platanus species (london plane, oriental plane and american sycamore) and has killed tens of thousands of plantation trees and street trees in the eastern united states, southern europe and modesto, california. nuclear and mitochondrial dna fingerprints and alleles of eight polymorphic microsatellite markers of isolates of c. fimbriata from these regions delineated major differences in gene diversities. the 33 isolates from th ... | 2004 | 15367115 |
| [determination of furanoterpenoid toxins from sweet potato by thin-layer chromatography]. | sweet potato root tissue, when infected with different pathogens, invaded by insects, or irritated by certain chemicals, turns black-brown and produces and accumulates a large amount of furanoterpenoids such as ipomeamarone, ipomeamaronol, ipomeanine and 4-ipomeanol, which are toxic to animals. the furanoterpenoids from sweet potato root tissues infected with ceratocystis fimbriata were extracted with ether. the extract solution was washed with 5% na2co3 and followed with distilled water and dri ... | 1997 | 15739468 |
| spoilage of vegetable crops by bacteria and fungi and related health hazards. | after harvest, vegetables are often spoiled by a wide variety of microorganisms including many bacterial and fungal species. the most common bacterial agents are erwinia carotovora, pseudomonas spp., corynebacterium, xanthomonas campestris, and lactic acid bacteria with e. carotovora being the most common, attacking virtually every vegetable type. fungi commonly causing spoilage of fresh vegetables are botrytis cinerea, various species of the genera alternaria, aspergillus, cladosporium, colleto ... | 2005 | 15839403 |
| post harvest spoilage of sweetpotato in tropics and control measures. | sweetpotato storage roots are subjected to several forms of post harvest spoilage in the tropical climate during transportation from farmers' field to market and in storage. these are due to mechanical injury, weight loss, sprouting, and pests and diseases. sweetpotato weevil is the single most important storage pest in tropical regions for which no control measures or resistant variety are yet available. several microorganisms (mostly fungi) have been found to induce spoilage in stored sweetpot ... | 2005 | 16371331 |
| ceratocystis omanensis, a new species from diseased mango trees in oman. | mango (mangifera indica) sudden decline is an important disease in oman, which is closely associated with infections by ceratocystis fimbriata and lasiodiplodia theobromae. another ceratocystis species has also been found associated with symptoms on diseased trees. in this study, we identify that ceratocystis based on morphology and dna sequences. morphological comparisons showed that the fungus from dying mango trees in oman is similar to c. moniliformis. both fungi have distinct hat-shaped asc ... | 2006 | 16388939 |
| intersterility, morphology and taxonomy of ceratocystis fimbriata on sweet potato, cacao and sycamore. | ceratocystis fimbriata is a large, diverse complex of species that cause wilt-type diseases of many economically important plants. previous studies have shown that isolates in three monophyletic lineages within the latin american clade of c. fimbriata are host-specialized to cacao (theobroma cacao), sweet potato (ipomoea batatas) and sycamore (platanus spp.), respectively. we paired testers of opposite mating type from isolates of these lineages to find intersterility groups. two intersterility ... | 2005 | 16389957 |
| phylogeny and taxonomy of the north american clade of the ceratocystis fimbriata complex. | ceratocystis fimbriata is a widely distributed, plant pathogenic fungus that causes wilts and cankers on many woody hosts. earlier phylogenetic analyses of dna sequences revealed three geographic clades within the c. fimbriata complex that are centered respectively in north america, latin america and asia. this study looked for cryptic species within the north american clade. the internal transcribed spacer regions (its) of the rdna were sequenced, and phylogenetic analysis indicated that most i ... | 2005 | 16596958 |
| analytical study of ipomeamarone & chlorogenic acid alterations in sweet potato roots infected by ceratocystis fimbriata. | | 1961 | 16655482 |
| pattern of carbohydrate breakdown in sweet potato roots infected with ceratocystis fimbriata. | | 1962 | 16655711 |
| analytical study of umbelliferone and scopoletin synthesis in sweet potato roots infected by ceratocystis fimbriata. | | 1963 | 16655821 |
| respiratory activity of the mitochondrial fractions isolated from healthy potato tubers and from tuber tissue incubated after cutting or infection with ceratocystis fimbriata. | | 1965 | 16656185 |
| increase of mitochondrial fraction in sweet potato root tissue after wounding or infection with ceratocystis fimbriata. | the acid-insoluble nitrogen content, lipid content, and cytochrome oxidase activity in the mitochondrial fraction are found to increase during incubation of slices of sweet potato (ipomoea batatas) root tissue. these increases appear to be related to an increase in the number of the mitochondrial particles. the increase in the mitochondrial fraction is not accompanied by an increase in cell number. the nitrogen content in the mitochondrial fraction increases prior to the changes in the activity ... | 1966 | 16656382 |
| increased disease resistance and enzyme activity induced by ethylene and ethylene production of black rot infected sweet potato tissue. | exposure of root tissue from a susceptible variety of sweet potato to low concentrations of ethylene induced a resistance to infection by ceratocystis fimbriata and an increase in the activity of peroxidase and polyphenoloxidase in the tissue. susceptible tissue that was inoculated with a pathogenic strain of c. fimbriata or a nonpathogenic strain that can induce resistance liberated more ethylene into closed chambers than tissue inoculated with strains that did not induce resistance. it is sugg ... | 1966 | 16656431 |
| effect of ethrel and ceratocystis fimbriata on the synthesis of fatty acids and 6-methoxy mellein in carrot root. | the rate of incorporation of (14)c from acetate-1-(14)c into fatty acids by carrot root discs, 18 hours after inoculation with ceratocystis fimbriata, was 9-fold greater than that in freshly cut discs. the rate in discs treated with water or ethrel was 3-fold greater. the rate of incorporation of (14)c from glucose-u-(13)c into fatty acids was 3-fold greater 18 hours after any of the above treatments. the rate of (14)c incorporation from malonate-2-(14)c into fatty acids 24 hours after inoculati ... | 1974 | 16658701 |
| dehydroipomeamarone as an intermediate in the biosynthesis of ipomeamarone, a phytoalexin from sweet potato root infected with ceratocystis fimbriata. | recently, we isolated dehydroipomeamarone, a new sesquiterpenoid from sweet potato (ipomoea batatas lam.) root tissue infected with ceratocystis fimbriata (ell. et halst.). the purpose of this investigation was to determine whether dehydroipomeamarone was a precursor in the biosynthetic pathway of ipomeamarone. the incorporation of acetate-2-(14)c into ipomeamarone was markedly inhibited by the presence of dehydroipomeamarone. radioactive dehydroipomeamarone was efficiently converted into ipomea ... | 1974 | 16658760 |
| induction of furano-terpene production and formation of the enzyme system from mevalonate to isopentenyl pyrophosphate in sweet potato root tissue injured by ceratocystis fimbriata and by toxic chemicals. | when sweet potato (ipomoea batatas) root tissue was infected by ceratocystis fimbriata, activity of the enzyme system from mevalonate to isopentenyl pyrophosphate, especially of pyrophosphomevalonate decarboxylase (ec 4.1.1.33), was increased in the noninfected tissue adjacent to the infected region, preceding the furano-terpene production in the infected region. cutting and incubation of sweet potato slices did not produce furano-terpenes, and only slightly increased the activity of the enzyme ... | 1976 | 16659619 |
| isolation and characterization of factors in sweet potato root which agglutinate germinated spores of ceratocystis fimbriata, black rot fungus. | a factor which agglutinates the germinated spores of ceratocystis fimbriata was isolated from the sweet potato root. the factor is a glycoprotein with a molecular weight of 1.6 x 10(6) daltons and required divalent cations such as ca(2+), mn(2+), ni(2+), and mg(2+) for activity. the activity of the factor was ph-dependent. the factor also agglutinated rabbit erythrocytes and is classified as a phytohemagglutinin or lectin. the factor agglutinated germinated spores of seven strains of c. fimbriat ... | 1978 | 16660599 |
| studies on a factor in sweet potato root which agglutinates spores of ceratocystis fimbriata, black rot fungus. | a factor which agglutinated the spores of ceratocystis fimbriata in the presence of ca(2+) was purified from sweet potato (ipomea batatas lam cv. norin[1]) root. element composition of the purified factor was as follows; analysis found: c (29.8%), h (3.97%), o (65.34%), n (0.81%): calculated for c(43)h(69)o(70)n(1): c (30.02%), h (4.01%), o (65.15%), n (0.81%). the factor was mainly composed of galacturonic acid (53% of dry weight) and contained arabinose, fucose, and unidentified component as m ... | 1982 | 16662232 |
| properties of a mixed function oxygenase catalyzing ipomeamarone 15-hydroxylation in microsomes from cut-injured and ceratocystis fimbriata-infected sweet potato root tissues. | ipomeamarone 15-hydroxylase activity was found in a microsomal fraction from cut-injured and ceratocystis fimbriata-infected sweet potato (ipomoea batatas lam. cv. norin no. 1) root tissues and its optimum ph was 8.0. the enzyme reaction required o(2) and nadph. the k(m) values calculated for ipomeamarone and nadh were approximately 60 and 2 micromolar, respectively. nadph alone had little effect on enzyme activity but activated the reaction in the presence of low concentrations of nadph. ipomea ... | 1982 | 16662536 |
| cerato-platanin, the first member of a new fungal protein family: cloning, expression, and characterization. | the ascomycete ceratocystis fimbriata, the causal agent of "canker stain disease," secretes a protein of 12.4 kda that elicits phytoalexin synthesis and plant cell death. this protein, named cerato-platanin (cp), is also located in the cell walls of ascospores, hyphae, and conidia; it contains four cysteines (s-s bridged) and is moderately hydrophobic. the cp gene consists of a single exon and has 42 bp codifying for a signal peptide of 14 residues. the recombinant protein was obtained by clonin ... | 2006 | 16679539 |
| 1h, 15n and 13c resonance assignments of cerato-platanin, a phytotoxic protein from ceratocystis fimbriata. | | 2006 | 16819587 |
| cerato-platanin, a phytotoxic protein from ceratocystis fimbriata: expression in pichia pastoris, purification and characterization. | cerato-platanin (cp) is a phytotoxic protein secreted by the ascomycete ceratocystis fimbriata f.sp. platani. this ascomycete causes canker stain which is a severe disease with a high incidence in the european platanus acerifolia. cp probably plays a role in the disease, eliciting defence-related responses in the host plants. cp is a 120 amino acid protein, containing 40% hydrophobic residues and two s-s bridges. in the embl data bank cp is the first member of a new fungal protein family known a ... | 2006 | 16931046 |
| epl1, the major secreted protein of hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors. | we used a proteomic approach to identify constitutively formed extracellular proteins of hypocrea atroviridis (trichoderma atroviride), a known biocontrol agent. the fungus was cultivated on glucose and the secretome was examined by two-dimensional gel electrophoresis. the two predominant spots were identified by maldi ms utilizing peptide mass fingerprints and amino acid sequence tags obtained by postsource decay and/or high-energy collision-induced dissociation (ms/ms) experiments, and turned ... | 2006 | 16939625 |
| atomic force microscopy images suggest aggregation mechanism in cerato-platanin. | cerato-platanin (cp), the first member of the "cerato-platanin family", is a moderately hydrophobic protein produced by ceratocystis fimbriata, the causal agent of a severe plant disease called "canker stain". the protein is localized in the cell wall of the fungus and it seems to be involved in the host-plane interaction and induces both cell necrosis and phytoalexin synthesis (one of the first plant defence-related events). recently, it has been determined that cp, like other fungal surface pr ... | 2007 | 17431609 |
| effect of synthetic polylysine on fungi. | the synthetic, basic poly-alpha-amino acid, polylysine, had antifungal activity against plant pathogens (three strains of fusaria, three isolates of verticillia, and ceratocystis fimbriata) and against the human pathogens (trichophyton mentagrophytes, t. rubrum, and candida albicans) in vitro. it inhibited penetration of ceratocystis fimbriata on sweet potato slices. polylysine inhibited the infection of tomato cuttings by flsarium oxysporum f. lycopersici, but it was also toxic to the plants. | 1960 | 17747266 |
| identification of a second family of genes in moniliophthora perniciosa, the causal agent of witches' broom disease in cacao, encoding necrosis-inducing proteins similar to cerato-platanins. | the hemibiotrophic basidiomycete moniliophthora perniciosa is the causal agent of witches' broom disease in cacao. this is a dimorphic species, with monokaryotic hyphae during the biotrophic phase, which is converted to dikaryotic mycelia during the saprophytic phase. the infection in pod is characterized by the formation of hypertrophic and hyperplasic tissues in the biotrophic phase, which is followed by necrosis and complete degradation of the organ. we found at least five sequences in the fu ... | 2009 | 18796332 |
| pathogenicity, internal transcribed spacer-rdna variation, and human dispersal of ceratocystis fimbriata on the family araceae. | abstract ceratocystis fimbriata is a complex of many cryptic, host-specialized species that causes wilt and canker of woody species and rot diseases of storage roots and corms of many economically important plants worldwide. with the exception of the family araceae, all confirmed hosts of c. fimbriata are dicotyledonous plants. we hypothesized that the isolates from members of the family araceae would form a monophyletic lineage specialized to infect these unique hosts. analyses of sequences of ... | 2005 | 18943126 |
| genetic variability and host specialization in the latin american clade of ceratocystis fimbriata. | abstract the ceratocystis fimbriata complex includes many undescribed species that cause wilt and canker diseases of many economically important plants. phylogenetic analyses of dna sequences have delineated three geographic clades within ceratocystis fimbriata. this study examined host specialization in the latin american clade, in which a number of lineages were identified using sequences of the internal transcribed spacer (its) region of the rdna. three host-associated lineages were identifie ... | 2003 | 18944327 |
| new proteins orthologous to cerato-platanin in various ceratocystis species and the purification and characterization of cerato-populin from ceratocystis populicola. | natural variants of cerato-platanin (cp), a pathogen associated molecular pattern (pamp) protein produced by ceratocystis platani (the causal agent of the plane canker stain), have been found to be produced by other four species of the genus ceratocystis, including five clones of ceratocystis fimbriata isolated from different hosts. all these fungal strains were known to be pathogenic to plants with considerable importance in agriculture, forestry, and as ornamental plants. the putative prematur ... | 2009 | 19387635 |
| microsatellite markers reflect intra-specific relationships between isolates of the vascular wilt pathogen ceratocystis fimbriata. | summary ceratocystis fimbriata is a serious wilt and canker stain pathogen with a wide geographical distribution and host range that includes both woody and herbaceous plants. previous studies using hybridization have shown that isolates of c. fimbriata from different hosts and origins differ in colony morphology, pathogenicity and growth rate, as well as conidial state. it has therefore been suggested that distinct strains, linked to host or geographical origin, are encompassed in c. fimbriata. ... | 2001 | 20573021 |
| genetic variation and variation in aggressiveness to native and exotic hosts among brazilian populations of ceratocystis fimbriata. | ceratocystis fimbriata is a complex of many species that cause wilt and cankers on woody plants and rot of storage roots or corms of many economically important crops worldwide. in brazil, c. fimbriata infects different cultivated crop plants that are not native to brazil, including gmelina arborea, eucalyptus spp., mangifera indica (mango), ficus carica (fig), and colocasia esculenta (inhame). phylogenetic analyses and inoculation studies were performed to test the hypothesis that there are hos ... | 2011 | 21190423 |
| movement of genotypes of ceratocystis fimbriata within and among eucalyptus plantations in brazil. | ceratocystis wilt on eucalyptus, caused by ceratocystis fimbriata, was first recognized in 1997 in the state of bahia, brazil, but is now known in five other states and in four other countries. c. fimbriata is a native, soilborne pathogen in some parts of brazil, but we hypothesized that genotypes of the pathogen have been moved among plantations in rooted cuttings collected from diseased trees and within plantations on cutting tools. we used six microsatellite markers to identify 78 genotypes o ... | 2011 | 21486144 |
| transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by ceratocystis fimbriata in leaves and storage roots. | black rot of sweet potato caused by pathogenic fungus ceratocystis fimbriata severely deteriorates both growth of plants and post-harvest storage. antimicrobial peptides from various organisms have broad range activities of killing bacteria, mycobacteria, and fungi. plant thionin peptide exhibited anti-fungal activity against c. fimbriata. a gene for barley α-hordothionin (αht) was placed downstream of a strong constitutive promoter of e12ω or the promoter of a sweet potato gene for β-amylase of ... | 2012 | 22212462 |